Coccidiosis is a protozoan disease that seriously threatens the poultry in-dustry. At present, the prevention and treatment of chicken coccidiosis mainly rely on chemical drugs and coccidia live vaccine. However, drug...Coccidiosis is a protozoan disease that seriously threatens the poultry in-dustry. At present, the prevention and treatment of chicken coccidiosis mainly rely on chemical drugs and coccidia live vaccine. However, drug control has problems of drug resistance and drug residue, and the coccidia live vaccine has drawbacks of pathogenicity, high production costs and virulence enhancement. Instead, genetic en-gineering vaccine is easy to operate, stable, safe and efficient, so it has become a hot spot in the prevention of chicken coccidiosis. In this paper, the research status, immune mechanism, immune effect＇s influencing factors and cytokine adjuvant appli-cation of genetic engineering vaccine against chicken coccidiosis were reviewed to provide some reference for the research and application of chicken coccidiosis gene vaccine.展开更多
[Objective] This study aimed to investigate the effects of traditional Chinese herbal medicine on the structure of duodenal mucosa of chickens under heat stress. [Method] One hundred and twenty 88-day-old Isa Brown ch...[Objective] This study aimed to investigate the effects of traditional Chinese herbal medicine on the structure of duodenal mucosa of chickens under heat stress. [Method] One hundred and twenty 88-day-old Isa Brown chickens were randomly divided into six groups, including three control groups （normal temperature control group, high temperature control group, high temperature Vc control group） and three high-temperature administration groups （high-dose administration group, moderate-dose administration group, low-dose administration group）. Chickens in normal temperature control group were reared at 14-25 ℃, and those in other five groups were reared at 28-39 ℃. The experiment lasted 10 d. Five chickens in each group were euthanized at 1, 4, 8 and 10 d post-treatment, respectively. The duodenal mucosa was collected and prepared into tissue slices with the conventional method for hematoxylin-eosin （HE） staining. Mucosal thickness, villus length and crypt depth of duodenal mucosa were measured. [Result] Under heat stress, duodenal mucosal thickness and villus length were both significantly lower than those in normal temperature control group, and the duodenal villi were scarce and thin. However, under high temperature conditions, various indicators in high-dose and moderate-dose administration groups were higher than those in high temperature control group and high temperature Vc control group, which exhibited extremely significant differences at 8 and 10 d post-treatment, especially. Moreover, various indicators in high-dose administration group were significantly higher than those in moderate-dose and low-dose administration groups. The traditional Chinese herbal medicine prescription exhibited higher protective effects on duodenal mucosa of chickens under heat stress compared with high temperature Vc control group. [Conclusion] Under heat stress, traditional Chinese herbal medicine reduced effectively the duodenal mucosa damage in chickens.展开更多
[Objective] This study was conducted to investigate the effects of tradi- tional Chinese herbal medicines on blood biochemical indices of egg-laying hens under heat stress and to provide theoretical basis for clinical...[Objective] This study was conducted to investigate the effects of tradi- tional Chinese herbal medicines on blood biochemical indices of egg-laying hens under heat stress and to provide theoretical basis for clinical application of the medicines. [Method] One hundred of 88-day-old ISA-Brown hens were randomly di- vided into five groups. The hens in four groups were administrated with Prescription I herbal medicines, Prescription II herbal medicines, Vc and blank control under ar- tificially simulated heat stress, respectively. The last group was raised under normal temperature condition. Blood samples of the hens were collected on 1, 4, 8 and 10 d later and detected for their biochemical indices. [Result] The blood sodium （Na）, phosphorus （P）, potassium （K） and chlorine （CI） concentrations in hens under heat stress were decreased, while the activities of alanine aminotransferase （ALT）, lactate dehydrogenase （LDH） and creatine kinase （CK） were increased. These indices grad- ually returned to normal levels after the use of traditional Chinese herbal medicines. [Conclusion] The herbal medicines can relieve the adverse effects caused by heat stress to blood biochemical indices via different metabolic pathways of ions and en- zymes.展开更多
Salt tolerance and freeze preservation of crucian are always important concerns of people. There are rare reports on effect of different salinity on fresh- ness and quality. However, water salinity will directly affec...Salt tolerance and freeze preservation of crucian are always important concerns of people. There are rare reports on effect of different salinity on fresh- ness and quality. However, water salinity will directly affect osmotic pressure in fish, thereby affecting meat quality. In this study, the tolerance of crucian to different salinities （1‰, 2‰, 5‰, 8‰, 10‰ and 15‰） was investigated. In addition, the ef- fect of different salinity on freezing freshness and quality of crucian was studied. The results showed that the tolerance threshold of crucian to salinity was about 8‰, and the freezing quality of crucian in water with salinity of ‰ was significantly better than that in fresh water.展开更多
Tilapia has wide-range tolerance to salinity, and most Tilapia species can survive in both fresh water and seawater. In this study, the survival rates, absolute growth rates and instantaneous growth rates of Tilapia n...Tilapia has wide-range tolerance to salinity, and most Tilapia species can survive in both fresh water and seawater. In this study, the survival rates, absolute growth rates and instantaneous growth rates of Tilapia nilotica and Tilapia mossambica in waters with four different salinities（0‰, 10‰, 20‰, 30‰） were investigated.The results showed that the growth of T. mossambica was trended to be increased with the increased salinity, and it is suitable to be farmed in seawater; however, the growth of T. nilotica was trended to be decreased with the increased salinity, and it is suitable to be farmed in fresh water.展开更多
The histopathological changes in various organs of Cynoglossus semilaevis Günther infected with red abdominal shell disease were observed by dissection and HE staining. In the ill C. semilaevis, the body surface ...The histopathological changes in various organs of Cynoglossus semilaevis Günther infected with red abdominal shell disease were observed by dissection and HE staining. In the ill C. semilaevis, the body surface was congestive and bleeding;the skin in the blind side ulcerated with extravasated blood; the gill filaments ulcerated with a lot of mucus; the liver, pancreas and spleen were swelling and bleeding with extravasated blood; the small intestine was congestive and bleeding. Degeneration and necrosis, and submucosal bleeding occurred in the skin of C. semilaevis infected with red abdominal shell disease. There was red blood cell infiltration and deposition in the space among muscle fibers. Epithelial degeneration and necrosis occurred in the parotid gland. The efferent branchial artery was congestive. The myocardial fibers had a loose structure, and a large area of fracture appeared. The liver cells were swelling with degeneration and necrosis and concentrated nucleus. A large area of the renal interstitial was broken down and necrotic. The submucosa of the small intestine had necrosis and hemorrhage.展开更多
基金Supported by Natural Science Foundation of Hebei Province(C2014407043)Educational Commission of Hebei Province(ZD2014071)+1 种基金Modern Agricultural IndustrialTechnology System of Hebei Province(HBCT2013090203)~~
文摘Coccidiosis is a protozoan disease that seriously threatens the poultry in-dustry. At present, the prevention and treatment of chicken coccidiosis mainly rely on chemical drugs and coccidia live vaccine. However, drug control has problems of drug resistance and drug residue, and the coccidia live vaccine has drawbacks of pathogenicity, high production costs and virulence enhancement. Instead, genetic en-gineering vaccine is easy to operate, stable, safe and efficient, so it has become a hot spot in the prevention of chicken coccidiosis. In this paper, the research status, immune mechanism, immune effect＇s influencing factors and cytokine adjuvant appli-cation of genetic engineering vaccine against chicken coccidiosis were reviewed to provide some reference for the research and application of chicken coccidiosis gene vaccine.
基金Supported by Agricultural Science and Technology Achievement Transformation Project of the Ministry of Science and Technology(2012GB2A200044)Project of Science and Technology Department of Hebei Province(13826615D)~~
文摘[Objective] This study aimed to investigate the effects of traditional Chinese herbal medicine on the structure of duodenal mucosa of chickens under heat stress. [Method] One hundred and twenty 88-day-old Isa Brown chickens were randomly divided into six groups, including three control groups （normal temperature control group, high temperature control group, high temperature Vc control group） and three high-temperature administration groups （high-dose administration group, moderate-dose administration group, low-dose administration group）. Chickens in normal temperature control group were reared at 14-25 ℃, and those in other five groups were reared at 28-39 ℃. The experiment lasted 10 d. Five chickens in each group were euthanized at 1, 4, 8 and 10 d post-treatment, respectively. The duodenal mucosa was collected and prepared into tissue slices with the conventional method for hematoxylin-eosin （HE） staining. Mucosal thickness, villus length and crypt depth of duodenal mucosa were measured. [Result] Under heat stress, duodenal mucosal thickness and villus length were both significantly lower than those in normal temperature control group, and the duodenal villi were scarce and thin. However, under high temperature conditions, various indicators in high-dose and moderate-dose administration groups were higher than those in high temperature control group and high temperature Vc control group, which exhibited extremely significant differences at 8 and 10 d post-treatment, especially. Moreover, various indicators in high-dose administration group were significantly higher than those in moderate-dose and low-dose administration groups. The traditional Chinese herbal medicine prescription exhibited higher protective effects on duodenal mucosa of chickens under heat stress compared with high temperature Vc control group. [Conclusion] Under heat stress, traditional Chinese herbal medicine reduced effectively the duodenal mucosa damage in chickens.
基金Supported by Agricultural Science and Technology Achievement Transformation Fund of Ministry of Science and Technology(2012GB2A200044)Fund from Department of Science and Technology of Hebei Province(13826615D)~~
文摘[Objective] This study was conducted to investigate the effects of tradi- tional Chinese herbal medicines on blood biochemical indices of egg-laying hens under heat stress and to provide theoretical basis for clinical application of the medicines. [Method] One hundred of 88-day-old ISA-Brown hens were randomly di- vided into five groups. The hens in four groups were administrated with Prescription I herbal medicines, Prescription II herbal medicines, Vc and blank control under ar- tificially simulated heat stress, respectively. The last group was raised under normal temperature condition. Blood samples of the hens were collected on 1, 4, 8 and 10 d later and detected for their biochemical indices. [Result] The blood sodium （Na）, phosphorus （P）, potassium （K） and chlorine （CI） concentrations in hens under heat stress were decreased, while the activities of alanine aminotransferase （ALT）, lactate dehydrogenase （LDH） and creatine kinase （CK） were increased. These indices grad- ually returned to normal levels after the use of traditional Chinese herbal medicines. [Conclusion] The herbal medicines can relieve the adverse effects caused by heat stress to blood biochemical indices via different metabolic pathways of ions and en- zymes.
基金Supported by Incentive Program Subsidized by Department of Science and Technology of Hebei Province(15926620H)Key Technology Research and Development Program of Science and Technology Bureau of Qinhuangdao City(201401A067201402B043)~~
文摘Salt tolerance and freeze preservation of crucian are always important concerns of people. There are rare reports on effect of different salinity on fresh- ness and quality. However, water salinity will directly affect osmotic pressure in fish, thereby affecting meat quality. In this study, the tolerance of crucian to different salinities （1‰, 2‰, 5‰, 8‰, 10‰ and 15‰） was investigated. In addition, the ef- fect of different salinity on freezing freshness and quality of crucian was studied. The results showed that the tolerance threshold of crucian to salinity was about 8‰, and the freezing quality of crucian in water with salinity of ‰ was significantly better than that in fresh water.
基金Supported by Incentive Program Subsidized by Department of Science and Technology of Hebei Province(15926620H)Key Technology Research and Development Program of Science and Technology Bureau of Qinhuangdao City(201401A067,201402B043)~~
文摘Tilapia has wide-range tolerance to salinity, and most Tilapia species can survive in both fresh water and seawater. In this study, the survival rates, absolute growth rates and instantaneous growth rates of Tilapia nilotica and Tilapia mossambica in waters with four different salinities（0‰, 10‰, 20‰, 30‰） were investigated.The results showed that the growth of T. mossambica was trended to be increased with the increased salinity, and it is suitable to be farmed in seawater; however, the growth of T. nilotica was trended to be decreased with the increased salinity, and it is suitable to be farmed in fresh water.
基金Supported by Incentive Program Subsidized by Department of Science and Technology of Hebei Province(15926620H)Key Technology Research and Development Program of Science and Technology Bureau of Qinhuangdao City(201401A067,201402B043)~~
文摘The histopathological changes in various organs of Cynoglossus semilaevis Günther infected with red abdominal shell disease were observed by dissection and HE staining. In the ill C. semilaevis, the body surface was congestive and bleeding;the skin in the blind side ulcerated with extravasated blood; the gill filaments ulcerated with a lot of mucus; the liver, pancreas and spleen were swelling and bleeding with extravasated blood; the small intestine was congestive and bleeding. Degeneration and necrosis, and submucosal bleeding occurred in the skin of C. semilaevis infected with red abdominal shell disease. There was red blood cell infiltration and deposition in the space among muscle fibers. Epithelial degeneration and necrosis occurred in the parotid gland. The efferent branchial artery was congestive. The myocardial fibers had a loose structure, and a large area of fracture appeared. The liver cells were swelling with degeneration and necrosis and concentrated nucleus. A large area of the renal interstitial was broken down and necrotic. The submucosa of the small intestine had necrosis and hemorrhage.
