A weight is placed on the top of a rectangular rigid ideal table with four legs, each leg is placed at each vertex of the rectangular table. It is assumed that the legs do not bend when the weight is added. The reacti...A weight is placed on the top of a rectangular rigid ideal table with four legs, each leg is placed at each vertex of the rectangular table. It is assumed that the legs do not bend when the weight is added. The reactions are computed by assuming the table is supported on a beam, introducing two new beam parameters and minimizing a deflection function of the new parameters. A physical experiment is performed in the lab and the reactions on each leg are provided. The experimental results match the theoretical ones obtained by the proposed model. Geometrical interpretations of the results are given.展开更多
Emerging contaminants constitute a set of substances that are released into the environment for which regulations are currently not established for their environmental monitoring, being antimony one of them. A new met...Emerging contaminants constitute a set of substances that are released into the environment for which regulations are currently not established for their environmental monitoring, being antimony one of them. A new methodology for Sb(III) traces monitoring by solid surface fluorescence is proposed. The metal was complexed with alizarine (Az) as fluorosphore reagent in alcaline medium in presence of the bile salt sodium cholate. To isolate the analyte of matrix constituents, a preconcentration/separation strategy on filter paper was introduced prior to determination step. The solid surface fluorescence was measured λem = 450 nm and λexc = 363 nm using a solid sampler holder. Under optimal conditions, the limits of detection and quantification of proposed methodology were 0.08 and 0.24 μg·L-1, respectively, showing a linear range from 0.24 to 304.4 μg·L-1 with good sensitivity and adequate selectivity. It was applied to the Sb(III) traces determination present in drinking water and beverages samples packaged in polyethylene terephthalate (PET) bottles widely consumed in Argentina. The combination of a preconcentration step on common filter paper and the inherent sensitivity of photoluminescent methods have permitted to achieve sensitivity similar to atomic spectroscopies using a lower price instrument typical in control laboratories. Precision and accuracy were tested with excellent agreement. Results were truenessed by ETAAS with satisfactory concordance.展开更多
The present study investigated the fauna of metazoan parasites of a Geophagus proximus population from the lower Tapajós River, in the state of Pará, northern Brazil. A total of 137 monogeneans were collecte...The present study investigated the fauna of metazoan parasites of a Geophagus proximus population from the lower Tapajós River, in the state of Pará, northern Brazil. A total of 137 monogeneans were collected from the gills of G. proximus, including Sciadicleithrum kritskyi, Sciadicleithrum paranaensis and Sciadicleithrum geophagi, while 119 Raphidascaris (Sprentascaris) lanfrediae nematodes and 28 metacercariaes of digenea undetermined were collected from the intestine. Hosts harboring four species of parasites were predominant. The parasites had an aggregated dispersion pattern. The present study represents the first record of S. geophagi parasitizing G. proximus, increasing the geographic distribution of these parasite species to the Tapajós River basin.展开更多
<strong>Background:</strong> Ischemia-reperfusion injury of organ transplantation activates several mediators which may link the innate to the adaptive immune response. Down the cascade of TLRs, we selecte...<strong>Background:</strong> Ischemia-reperfusion injury of organ transplantation activates several mediators which may link the innate to the adaptive immune response. Down the cascade of TLRs, we selected to study the expression of Interferon Regulatory Factors (IRF)-3 and -7 inside human Kidney Transplanted (KTx) organs and the synthesis of IFN<i>α</i>, the main growth factor induced by them, in KTx aspiration biopsy cultures. Simultaneously, we tested their robustness in diagnosing Acute Rejection (AR). <strong>Methods:</strong> Fine-needle aspiration biopsies (F-nab) were performed either on day 7 or 14 post-KTx among stable patients or on the day of AR diagnosis. On Fnab cytopreparations, we studied IRF3 and IRF7 by the enzymatic avidin-biotin complex staining, and in a different group of cases we quantified IFN<i>α</i> by ELISA in 48 hours Fnab culture supernatants. <strong>Results:</strong> AR group showed a significantly up-regulated expression for IRF3 and IRF7, reaching Positive Predictive Values (PPV) of 0.824 and 0.8, respectively, as well as Negative Predictive Values (NPV) above 0.9 for both;IFN<i>α</i> presented a PPV = 1.0 and a NPV = 0.9. A variation in the results was noticed according to different immunosuppressive therapies. <strong>Conclusions:</strong> Our findings suggest that IRF3 and IRF7, and IFN<i>α</i> which they promote, may be very important players in the early days post-KTx, linking the innate with an adaptive response and triggering acute rejection. These differences were very clear-cut, lending consistency to our speculation. It would be important to scrutinize for other potential effects derived from these IRFs up-regulation which could be of clinical relevance.展开更多
<b><span style="font-family:Verdana;">Introduction</span></b><span "=""><span style="font-family:Verdana;">: Degeneration of the cervical spine (C...<b><span style="font-family:Verdana;">Introduction</span></b><span "=""><span style="font-family:Verdana;">: Degeneration of the cervical spine (CDSD) prevalence is nearly 90% by the 7th decade. This is the first research that compares the outcomes between the Anterior Approach (AA) and Posterior Approach (PA) to cervical myelopathy (CM) in the elderly. </span><b><span style="font-family:Verdana;">Materials and Methods</span></b><span style="font-family:Verdana;">: A retrospective observational study of electronic health records at the Hospital Central Sur de Alta Especialidad (HCSAE), PEMEX from January 2010 to May 2020 with patients older than 60 years submitted to cervical surgery. For the analysis we elaborated two groups according to the surgical approach: AA vs PA;we analyzed the trans-operative behavior, the immediate outcome, and after 3 months, 6 months, and 1 year. </span><b><span style="font-family:Verdana;">Results</span></b><span style="font-family:Verdana;">: </span></span><span style="font-family:Verdana;">As </span><span style="font-family:Verdana;">a total of 145 patients, the prevalence of CM in elderly was the 63.8% with a median age of 69 (64 - 75) years. We found statistical differences in strength outcome only in the P3m (p = 0.011), for sensitivity</span><span "=""> </span><span style="font-family:Verdana;">we found major prevalence of affection in the PA group. We didn’t report a significant difference in the Neck Disability Index (NDI) at all measures, but the PA presented a major incapacity. The Nurick scale results were significant in all stages (p < 0.05);and presented improvement compared with presurgical period (p < 0.001)</span><span style="font-family:Verdana;">.</span><span "=""> <b><span style="font-family:Verdana;">Discussion</span></b><span style="font-family:Verdana;">: Patients show significant improvements in outcome measures with either anterior or posterior surgery. Both approaches are highly efficacious in preventing neurologic deterioration and in most cases improve neurological function with appropriate postoperative management like rehabilitation, pain management</span></span><span style="font-family:Verdana;">,</span><span "=""><span style="font-family:Verdana;"> and psychological support. </span><b><span style="font-family:Verdana;">Conclusion</span></b><span style="font-family:Verdana;">: The patients submitted to surgical medullary decompression presented a favorable outcome despite the age and the higher prevalence of comorbidities;whereby we favor the surgical treatment in all patients in a case-to-case selection to generate a positive impact on functional outcomes.展开更多
文摘A weight is placed on the top of a rectangular rigid ideal table with four legs, each leg is placed at each vertex of the rectangular table. It is assumed that the legs do not bend when the weight is added. The reactions are computed by assuming the table is supported on a beam, introducing two new beam parameters and minimizing a deflection function of the new parameters. A physical experiment is performed in the lab and the reactions on each leg are provided. The experimental results match the theoretical ones obtained by the proposed model. Geometrical interpretations of the results are given.
文摘Emerging contaminants constitute a set of substances that are released into the environment for which regulations are currently not established for their environmental monitoring, being antimony one of them. A new methodology for Sb(III) traces monitoring by solid surface fluorescence is proposed. The metal was complexed with alizarine (Az) as fluorosphore reagent in alcaline medium in presence of the bile salt sodium cholate. To isolate the analyte of matrix constituents, a preconcentration/separation strategy on filter paper was introduced prior to determination step. The solid surface fluorescence was measured λem = 450 nm and λexc = 363 nm using a solid sampler holder. Under optimal conditions, the limits of detection and quantification of proposed methodology were 0.08 and 0.24 μg·L-1, respectively, showing a linear range from 0.24 to 304.4 μg·L-1 with good sensitivity and adequate selectivity. It was applied to the Sb(III) traces determination present in drinking water and beverages samples packaged in polyethylene terephthalate (PET) bottles widely consumed in Argentina. The combination of a preconcentration step on common filter paper and the inherent sensitivity of photoluminescent methods have permitted to achieve sensitivity similar to atomic spectroscopies using a lower price instrument typical in control laboratories. Precision and accuracy were tested with excellent agreement. Results were truenessed by ETAAS with satisfactory concordance.
文摘The present study investigated the fauna of metazoan parasites of a Geophagus proximus population from the lower Tapajós River, in the state of Pará, northern Brazil. A total of 137 monogeneans were collected from the gills of G. proximus, including Sciadicleithrum kritskyi, Sciadicleithrum paranaensis and Sciadicleithrum geophagi, while 119 Raphidascaris (Sprentascaris) lanfrediae nematodes and 28 metacercariaes of digenea undetermined were collected from the intestine. Hosts harboring four species of parasites were predominant. The parasites had an aggregated dispersion pattern. The present study represents the first record of S. geophagi parasitizing G. proximus, increasing the geographic distribution of these parasite species to the Tapajós River basin.
文摘<strong>Background:</strong> Ischemia-reperfusion injury of organ transplantation activates several mediators which may link the innate to the adaptive immune response. Down the cascade of TLRs, we selected to study the expression of Interferon Regulatory Factors (IRF)-3 and -7 inside human Kidney Transplanted (KTx) organs and the synthesis of IFN<i>α</i>, the main growth factor induced by them, in KTx aspiration biopsy cultures. Simultaneously, we tested their robustness in diagnosing Acute Rejection (AR). <strong>Methods:</strong> Fine-needle aspiration biopsies (F-nab) were performed either on day 7 or 14 post-KTx among stable patients or on the day of AR diagnosis. On Fnab cytopreparations, we studied IRF3 and IRF7 by the enzymatic avidin-biotin complex staining, and in a different group of cases we quantified IFN<i>α</i> by ELISA in 48 hours Fnab culture supernatants. <strong>Results:</strong> AR group showed a significantly up-regulated expression for IRF3 and IRF7, reaching Positive Predictive Values (PPV) of 0.824 and 0.8, respectively, as well as Negative Predictive Values (NPV) above 0.9 for both;IFN<i>α</i> presented a PPV = 1.0 and a NPV = 0.9. A variation in the results was noticed according to different immunosuppressive therapies. <strong>Conclusions:</strong> Our findings suggest that IRF3 and IRF7, and IFN<i>α</i> which they promote, may be very important players in the early days post-KTx, linking the innate with an adaptive response and triggering acute rejection. These differences were very clear-cut, lending consistency to our speculation. It would be important to scrutinize for other potential effects derived from these IRFs up-regulation which could be of clinical relevance.
文摘<b><span style="font-family:Verdana;">Introduction</span></b><span "=""><span style="font-family:Verdana;">: Degeneration of the cervical spine (CDSD) prevalence is nearly 90% by the 7th decade. This is the first research that compares the outcomes between the Anterior Approach (AA) and Posterior Approach (PA) to cervical myelopathy (CM) in the elderly. </span><b><span style="font-family:Verdana;">Materials and Methods</span></b><span style="font-family:Verdana;">: A retrospective observational study of electronic health records at the Hospital Central Sur de Alta Especialidad (HCSAE), PEMEX from January 2010 to May 2020 with patients older than 60 years submitted to cervical surgery. For the analysis we elaborated two groups according to the surgical approach: AA vs PA;we analyzed the trans-operative behavior, the immediate outcome, and after 3 months, 6 months, and 1 year. </span><b><span style="font-family:Verdana;">Results</span></b><span style="font-family:Verdana;">: </span></span><span style="font-family:Verdana;">As </span><span style="font-family:Verdana;">a total of 145 patients, the prevalence of CM in elderly was the 63.8% with a median age of 69 (64 - 75) years. We found statistical differences in strength outcome only in the P3m (p = 0.011), for sensitivity</span><span "=""> </span><span style="font-family:Verdana;">we found major prevalence of affection in the PA group. We didn’t report a significant difference in the Neck Disability Index (NDI) at all measures, but the PA presented a major incapacity. The Nurick scale results were significant in all stages (p < 0.05);and presented improvement compared with presurgical period (p < 0.001)</span><span style="font-family:Verdana;">.</span><span "=""> <b><span style="font-family:Verdana;">Discussion</span></b><span style="font-family:Verdana;">: Patients show significant improvements in outcome measures with either anterior or posterior surgery. Both approaches are highly efficacious in preventing neurologic deterioration and in most cases improve neurological function with appropriate postoperative management like rehabilitation, pain management</span></span><span style="font-family:Verdana;">,</span><span "=""><span style="font-family:Verdana;"> and psychological support. </span><b><span style="font-family:Verdana;">Conclusion</span></b><span style="font-family:Verdana;">: The patients submitted to surgical medullary decompression presented a favorable outcome despite the age and the higher prevalence of comorbidities;whereby we favor the surgical treatment in all patients in a case-to-case selection to generate a positive impact on functional outcomes.
