目的:为探究外周血Zonulin在评估系统性红斑狼疮(SLE)患者肠道通透性、疾病活动度以及受累脏器的临床价值。方法:共纳入60名SLE女性患者,通过ELISA测量血清连蛋白(Zonulin)及脂肪酸结合蛋白(IFABP)水平,并收集系统性红斑狼疮疾病活动指...目的:为探究外周血Zonulin在评估系统性红斑狼疮(SLE)患者肠道通透性、疾病活动度以及受累脏器的临床价值。方法:共纳入60名SLE女性患者,通过ELISA测量血清连蛋白(Zonulin)及脂肪酸结合蛋白(IFABP)水平,并收集系统性红斑狼疮疾病活动指数(SLEDAI-2k)评分及临床指标评估疾病活动度,通过线性回归和Pearson相关性评估外周血Zonulin、IFABP水平与SLEDAI-2k及临床指标之间的相关性。结果:SLE患者中外周血Zonulin (p p p p 2 = 0.5247, p p p Objective: To explore the clinical value of peripheral blood Zonulin in the evaluation of intestinal permeability, disease activity, and affected organs in patients with systemic lupus erythematosus (SLE). Methods: 60 female patients with SLE were enrolled. Serum levels of Zonulin and intestinal fatty acid-binding protein (IFABP) were measured by ELISA. The SLE Disease Activity Index (SLEDAI-2k) score and clinical indicators were collected to assess disease activity. Linear regression and Pearson correlation were used to evaluate the correlations between Zonulin, IFABP in peripheral blood and SLEDAI-2k, clinical indicators. Results: Compared to healthy controls, the levels of Zonulin (p p p p 2 = 0.5247, p p p < 0.001) was negatively correlated. However, there was no statistically significant difference in Zonulin and IFABP levels in peripheral blood among patients with different organ involvements. Conclusion: Zonulin in peripheral blood may be associated with disease activity and exacerbation, potentially serving as a biomarker for the severity and progression of SLE, but it is unrelated to the involved organs and systems.展开更多
AIM: To investigate the potential association of circulating zonulin with the stage of liver disease in obese children with biopsy-confirmed nonalcoholic fatty liver disease (NAFLD).
BACKGROUND Increased gut permeability and bacterial translocation play an important role in liver cirrhosis.Zonulin is a recently recognized protein involved in the disintegration of the intestinal barrier.AIM To inve...BACKGROUND Increased gut permeability and bacterial translocation play an important role in liver cirrhosis.Zonulin is a recently recognized protein involved in the disintegration of the intestinal barrier.AIM To investigate possible differences in serum zonulin levels among patients with different cirrhosis stages and their potential prognostic implications.METHODS Consecutive cirrhotic patients who attended our liver clinic were included in the study.Serum zonulin levels,clinical,radiological and biochemical data were collected at baseline.Patients who accepted participation in a regular surveillance program were followed-up for at least 12 mo.RESULTS We enrolled 116 cirrhotics[mean Child-Turcotte-Pugh(CTP)score:6.2±1.6;model for end-stage liver disease score:11±3.9].The causes of cirrhosis were viral hepatitis(39%),alcohol(30%),non-alcoholic fatty liver disease(17%),and other(14%).At baseline,53% had decompensated cirrhosis,48% had ascites,and 32% had history of hepatic encephalopathy.Mean zonulin levels were significantly higher in patients with CTP-B class than CTP-A class(4.2±2.4 ng/dL vs 3.5±0.9 ng/dL,P=0.038),with than without ascites(P=0.006),and with than without history of encephalopathy(P=0.011).Baseline serum zonulin levels were independently associated with the probability of decompensation at 1 year(P=0.039),with an area under the receiving operating characteristic of 0.723 for predicting hepatic decompensation.Higher CTP score(P=0.021)and portal vein diameter(P=0.022)were independent predictors of mortality.CONCLUSION Serum zonulin levels are higher in patients with more advanced chronic liver disease and have significant prognostic value in identifying patients who will develop decompensation.展开更多
To evaluate the measurement of zonulin level and antibodies of zonulin and other tight junction proteins in the blood of controls and celiac disease patients.METHODSThis study was conducted to assess the variability o...To evaluate the measurement of zonulin level and antibodies of zonulin and other tight junction proteins in the blood of controls and celiac disease patients.METHODSThis study was conducted to assess the variability or stability of zonulin levels vs IgA and IgG antibodies against zonulin in blood samples from 18 controls at 0,6,24 and 30 h after blood draw.We also measured zonulin level as well as zonulin,occludin,vinculin,aquaporin 4 and glial fibrillary acidic protein antibodies in the sera of 30 patients with celiac disease and 30 controls using enzyme-linked immunosorbent assay methodology.RESULTSThe serum zonulin level in 6 out of 18 subjects was low or<2.8 ng/mL and was very close to the detection limit of the assay.The other 12 subjects had zonulin levels of>2.8 ng/mL and showed significant fluctuation from sample to sample.Comparatively,zonulin antibody measured in all samples was highly stable and reproducible from sample to sample.Celiac disease patients showed zonulin levels with a mean of 8.5 ng/mL compared to 3.7 ng/mL in controls(P<0.0001).Elevation of zonulin level at 2SD above the mean was demonstrated in 37%of celiac disease patients,while antibodies against zonulin,occludin and other tight junction proteins was detected in up to 86%of patients with celiac disease.CONCLUSIONDue to its fluctuation,a single measurement of zonulin level is not recommended for assessment of intestinal barrier integrity.Measurement of IgG and IgA antibodies against zonulin,occludin,and other tight junction proteins is proposed for the evaluation of the loss of intestinal barrier integrity.展开更多
为探讨EB病毒(Epstein-Barr virus,EBV)感染与溃疡性结肠炎(Ulcerative colitis,UC)患者血清连蛋白(zonulin)、白细胞介素-22(Interleukin-22,IL-22)水平的关系及对病情严重程度的影响,本研究选取2020年1月-2024年12月收治的UC患者195例...为探讨EB病毒(Epstein-Barr virus,EBV)感染与溃疡性结肠炎(Ulcerative colitis,UC)患者血清连蛋白(zonulin)、白细胞介素-22(Interleukin-22,IL-22)水平的关系及对病情严重程度的影响,本研究选取2020年1月-2024年12月收治的UC患者195例,经EBV-DNA检测后,根据其是否伴有EBV感染分为EBV感染组(121例)和无EBV感染组(74例)。采用改良Mayo评分评价患者病情严重程度,195例患者中轻中症患者123例,重症患者72例。检测UC患者血清zonulin、IL-22水平。采用Spearman分析EBV感染与UC患者血清zonulin及IL-22水平的相关性,UC患者病情严重程度与EBV感染、血清zonulin、IL-22水平的相关性。采用logistic回归分析UC患者病情的影响因素。采用受试者工作(Receiver operating characteristic,ROC)曲线分析EBV感染、血清zonulin、IL-22水平对UC患者病情的评估价值。结果显示,EBV感染组血清zonulin、IL-22水平高于无EBV感染组(P<0.05)。EBV感染与UC患者血清zonulin、IL-22水平呈正相关(P<0.05)。重症UC患者血清zonulin、IL-22水平及EBV感染比例高于轻中症患者(P<0.05)。UC患者病情严重程度与EBV感染、血清zonulin、IL-22水平呈正相关(P<0.05)。EBV感染、血清zonulin水平、血清IL-22水平为UC患者病情严重程度的独立影响因素(P<0.05)。EBV感染、血清zonulin、IL-22水平、三种指标联合UC患者病情为重症的曲线下面积(Area Under the Curve,AUC)分别为0.724、0.781、0.826、0.914,约登指数分别为0.448、0.475、0.576、0.722。以上结果表明,EBV感染与UC患者血清zonulin、IL-22水平相关,EBV感染、血清zonulin、IL-22水平是UC患者病情严重程度的影响因素,EBV感染、血清zonulin、IL-22水平有作为UC患者病情评估指标的潜力。展开更多
文摘目的:为探究外周血Zonulin在评估系统性红斑狼疮(SLE)患者肠道通透性、疾病活动度以及受累脏器的临床价值。方法:共纳入60名SLE女性患者,通过ELISA测量血清连蛋白(Zonulin)及脂肪酸结合蛋白(IFABP)水平,并收集系统性红斑狼疮疾病活动指数(SLEDAI-2k)评分及临床指标评估疾病活动度,通过线性回归和Pearson相关性评估外周血Zonulin、IFABP水平与SLEDAI-2k及临床指标之间的相关性。结果:SLE患者中外周血Zonulin (p p p p 2 = 0.5247, p p p Objective: To explore the clinical value of peripheral blood Zonulin in the evaluation of intestinal permeability, disease activity, and affected organs in patients with systemic lupus erythematosus (SLE). Methods: 60 female patients with SLE were enrolled. Serum levels of Zonulin and intestinal fatty acid-binding protein (IFABP) were measured by ELISA. The SLE Disease Activity Index (SLEDAI-2k) score and clinical indicators were collected to assess disease activity. Linear regression and Pearson correlation were used to evaluate the correlations between Zonulin, IFABP in peripheral blood and SLEDAI-2k, clinical indicators. Results: Compared to healthy controls, the levels of Zonulin (p p p p 2 = 0.5247, p p p < 0.001) was negatively correlated. However, there was no statistically significant difference in Zonulin and IFABP levels in peripheral blood among patients with different organ involvements. Conclusion: Zonulin in peripheral blood may be associated with disease activity and exacerbation, potentially serving as a biomarker for the severity and progression of SLE, but it is unrelated to the involved organs and systems.
文摘AIM: To investigate the potential association of circulating zonulin with the stage of liver disease in obese children with biopsy-confirmed nonalcoholic fatty liver disease (NAFLD).
文摘BACKGROUND Increased gut permeability and bacterial translocation play an important role in liver cirrhosis.Zonulin is a recently recognized protein involved in the disintegration of the intestinal barrier.AIM To investigate possible differences in serum zonulin levels among patients with different cirrhosis stages and their potential prognostic implications.METHODS Consecutive cirrhotic patients who attended our liver clinic were included in the study.Serum zonulin levels,clinical,radiological and biochemical data were collected at baseline.Patients who accepted participation in a regular surveillance program were followed-up for at least 12 mo.RESULTS We enrolled 116 cirrhotics[mean Child-Turcotte-Pugh(CTP)score:6.2±1.6;model for end-stage liver disease score:11±3.9].The causes of cirrhosis were viral hepatitis(39%),alcohol(30%),non-alcoholic fatty liver disease(17%),and other(14%).At baseline,53% had decompensated cirrhosis,48% had ascites,and 32% had history of hepatic encephalopathy.Mean zonulin levels were significantly higher in patients with CTP-B class than CTP-A class(4.2±2.4 ng/dL vs 3.5±0.9 ng/dL,P=0.038),with than without ascites(P=0.006),and with than without history of encephalopathy(P=0.011).Baseline serum zonulin levels were independently associated with the probability of decompensation at 1 year(P=0.039),with an area under the receiving operating characteristic of 0.723 for predicting hepatic decompensation.Higher CTP score(P=0.021)and portal vein diameter(P=0.022)were independent predictors of mortality.CONCLUSION Serum zonulin levels are higher in patients with more advanced chronic liver disease and have significant prognostic value in identifying patients who will develop decompensation.
文摘To evaluate the measurement of zonulin level and antibodies of zonulin and other tight junction proteins in the blood of controls and celiac disease patients.METHODSThis study was conducted to assess the variability or stability of zonulin levels vs IgA and IgG antibodies against zonulin in blood samples from 18 controls at 0,6,24 and 30 h after blood draw.We also measured zonulin level as well as zonulin,occludin,vinculin,aquaporin 4 and glial fibrillary acidic protein antibodies in the sera of 30 patients with celiac disease and 30 controls using enzyme-linked immunosorbent assay methodology.RESULTSThe serum zonulin level in 6 out of 18 subjects was low or<2.8 ng/mL and was very close to the detection limit of the assay.The other 12 subjects had zonulin levels of>2.8 ng/mL and showed significant fluctuation from sample to sample.Comparatively,zonulin antibody measured in all samples was highly stable and reproducible from sample to sample.Celiac disease patients showed zonulin levels with a mean of 8.5 ng/mL compared to 3.7 ng/mL in controls(P<0.0001).Elevation of zonulin level at 2SD above the mean was demonstrated in 37%of celiac disease patients,while antibodies against zonulin,occludin and other tight junction proteins was detected in up to 86%of patients with celiac disease.CONCLUSIONDue to its fluctuation,a single measurement of zonulin level is not recommended for assessment of intestinal barrier integrity.Measurement of IgG and IgA antibodies against zonulin,occludin,and other tight junction proteins is proposed for the evaluation of the loss of intestinal barrier integrity.
文摘为探讨EB病毒(Epstein-Barr virus,EBV)感染与溃疡性结肠炎(Ulcerative colitis,UC)患者血清连蛋白(zonulin)、白细胞介素-22(Interleukin-22,IL-22)水平的关系及对病情严重程度的影响,本研究选取2020年1月-2024年12月收治的UC患者195例,经EBV-DNA检测后,根据其是否伴有EBV感染分为EBV感染组(121例)和无EBV感染组(74例)。采用改良Mayo评分评价患者病情严重程度,195例患者中轻中症患者123例,重症患者72例。检测UC患者血清zonulin、IL-22水平。采用Spearman分析EBV感染与UC患者血清zonulin及IL-22水平的相关性,UC患者病情严重程度与EBV感染、血清zonulin、IL-22水平的相关性。采用logistic回归分析UC患者病情的影响因素。采用受试者工作(Receiver operating characteristic,ROC)曲线分析EBV感染、血清zonulin、IL-22水平对UC患者病情的评估价值。结果显示,EBV感染组血清zonulin、IL-22水平高于无EBV感染组(P<0.05)。EBV感染与UC患者血清zonulin、IL-22水平呈正相关(P<0.05)。重症UC患者血清zonulin、IL-22水平及EBV感染比例高于轻中症患者(P<0.05)。UC患者病情严重程度与EBV感染、血清zonulin、IL-22水平呈正相关(P<0.05)。EBV感染、血清zonulin水平、血清IL-22水平为UC患者病情严重程度的独立影响因素(P<0.05)。EBV感染、血清zonulin、IL-22水平、三种指标联合UC患者病情为重症的曲线下面积(Area Under the Curve,AUC)分别为0.724、0.781、0.826、0.914,约登指数分别为0.448、0.475、0.576、0.722。以上结果表明,EBV感染与UC患者血清zonulin、IL-22水平相关,EBV感染、血清zonulin、IL-22水平是UC患者病情严重程度的影响因素,EBV感染、血清zonulin、IL-22水平有作为UC患者病情评估指标的潜力。
