One of the ways to improve the quality of Indonesian Durian is by utilizing germplasm diversity. Durio zibethinus is the most cultivated durian in Indonesia, whereas Durio kutejensis is a unique durian cultivar which ...One of the ways to improve the quality of Indonesian Durian is by utilizing germplasm diversity. Durio zibethinus is the most cultivated durian in Indonesia, whereas Durio kutejensis is a unique durian cultivar which has golden yellow fruit flesh without smell. Crossbreeding of those two cultivars, in order to generate superior Durian cultivars has been done. Genetic diversity of durian generates from cross breeding between D. kutejensis and D. zibethinus was identified in molecular level using RAPD technique. Among 20 primers used in this study, 5 primers: OPA-02, OPA-03, OPA-08, OPA-10 and OPA-13 were capable of differentiating both the parents and the hybrids. RAPD analysis resulted in genetic diversity of hybrid Durian with family relationship of 0.59%-0.1%. Hybrids UB1, UB5, UB13, UB19, UB21, UB7 and UB35 have similarity value of 0.81% with their parent DRCK, whereas hybrids UB8, UB10, UB18 and UB17 have similarity value of 0.70% with their parent DRCM1. Hybrids UB2, UB16 and UB22 belong to one group with similarity value of 0.67%. Three hybrids lines UB2, UB16 and UB22 show the highest distance to both parent. The rest of the hybrids lines grouped into similar cluster to the parents D. kutejensis, whereas the other parent (D. zibethinus) belong to different cluster separated from all other hybrid lines and parents.展开更多
The physicochemical analysis was carried out to quantify the total soluble solids, acidity, pH, ascorbic acid contents and pulp colour of eight selected durian hybrid clones. The aim of this study was to select the mo...The physicochemical analysis was carried out to quantify the total soluble solids, acidity, pH, ascorbic acid contents and pulp colour of eight selected durian hybrid clones. The aim of this study was to select the most promising durian hybrids for commercialization. The durian hybrid fruits were collected in an established durian research plot. All tested characteristics with the exception for total soluble solids （TSS） showed significant differences （P 〈 0.05） amongst the eight durian hybrids with four check cultivars. F1 hybrid 15-3 had significantly higher pH （7.25） and lower titratable acidity （0.09%） than other hybrids. While for ascorbic acid content, durian hybrid 7-3 （26.13 rag/100 g） showed significantly higher than other hybrids. As for pulp colour, hybrids 1-15, 1-17 and 7-20 were yellowish orange/deep yellow. Even though there was no significant differences for TSS, three durian hybrids showed value above 39.00 °Brix and there were hybrid 14-3 （39.97 °Brix）, hybrid 8-16 （39.83 °Brix） and hybrid 7-3 （39.50 °Brix）. Based on the results of this study, Fl hybrids durian 1-15, 7-3, 8-16, 14-3 and 15-3 shall become successful candidates for recommendation to farmers for commercial production in Malaysia. However, further evaluations on fruit eating quality and stability of the hybrids should be completed prior to recommendation for commercialization.展开更多
Durian(Durio zibethinus)is a tropical fruit that has a unique flavor and aroma.It occupies a significant phylogenetic position within the Malvaceae family.Extant core-eudicot plants are reported to share seven ancestr...Durian(Durio zibethinus)is a tropical fruit that has a unique flavor and aroma.It occupies a significant phylogenetic position within the Malvaceae family.Extant core-eudicot plants are reported to share seven ancestral karyotypes that have undergone reshuffling,resulting in an abundant genomic diversity.However,the ancestral karyotypes of the Malvaceae family,as well as the evolution trajectory leading to the28 chromosomes in durian,remain poorly understood.Here,we report the high-quality assembly of the durian genome with comprehensive comparative genomic analyses.By analyzing the collinear blocks between cacao and durian,we inferred 11 Malvaceae ancestral karyotypes.These blocks were present in a single-copy form in cacao and mainly in triplicates in durian,possibly resulting from a recent whole genome triplication(WGT)event that led to hexaploidization of the durian genome around 20(17–24)million years ago.A large proportion of the duplicated genes in durian,such as those involved in the lignin biosynthesis module for phenylpropane biosynthesis,are derived directly from whole genome duplication,which makes it an important force in reshaping its genomic architecture.Transcriptome studies have revealed that genes involved in feruloyl-Co A formations were highly preferentially expressed in fruit peels,indicating that the thorns produced on durian fruit may comprise guaiacyl and syringyl lignins.Among all the analyzed transcription factors(TFs),members of the heat shock factor family(HSF)were the most significantly upregulated under heat stress.All subfamilies of genes encoding heat shock proteins(HSPs)in the durian genome appear to have undergone expansion.The potential interactions between HSF Dzi05.397 and HSPs were examined and experimentally verified.Our study provides a high-quality durian genome and reveals the reshuffling mechanism of ancestral Malvaceae chromosomes to produce the durian genome.We also provide insights into the mechanism underlying lignin biosynthesis and heat stress tolerance.展开更多
文摘One of the ways to improve the quality of Indonesian Durian is by utilizing germplasm diversity. Durio zibethinus is the most cultivated durian in Indonesia, whereas Durio kutejensis is a unique durian cultivar which has golden yellow fruit flesh without smell. Crossbreeding of those two cultivars, in order to generate superior Durian cultivars has been done. Genetic diversity of durian generates from cross breeding between D. kutejensis and D. zibethinus was identified in molecular level using RAPD technique. Among 20 primers used in this study, 5 primers: OPA-02, OPA-03, OPA-08, OPA-10 and OPA-13 were capable of differentiating both the parents and the hybrids. RAPD analysis resulted in genetic diversity of hybrid Durian with family relationship of 0.59%-0.1%. Hybrids UB1, UB5, UB13, UB19, UB21, UB7 and UB35 have similarity value of 0.81% with their parent DRCK, whereas hybrids UB8, UB10, UB18 and UB17 have similarity value of 0.70% with their parent DRCM1. Hybrids UB2, UB16 and UB22 belong to one group with similarity value of 0.67%. Three hybrids lines UB2, UB16 and UB22 show the highest distance to both parent. The rest of the hybrids lines grouped into similar cluster to the parents D. kutejensis, whereas the other parent (D. zibethinus) belong to different cluster separated from all other hybrid lines and parents.
文摘The physicochemical analysis was carried out to quantify the total soluble solids, acidity, pH, ascorbic acid contents and pulp colour of eight selected durian hybrid clones. The aim of this study was to select the most promising durian hybrids for commercialization. The durian hybrid fruits were collected in an established durian research plot. All tested characteristics with the exception for total soluble solids （TSS） showed significant differences （P 〈 0.05） amongst the eight durian hybrids with four check cultivars. F1 hybrid 15-3 had significantly higher pH （7.25） and lower titratable acidity （0.09%） than other hybrids. While for ascorbic acid content, durian hybrid 7-3 （26.13 rag/100 g） showed significantly higher than other hybrids. As for pulp colour, hybrids 1-15, 1-17 and 7-20 were yellowish orange/deep yellow. Even though there was no significant differences for TSS, three durian hybrids showed value above 39.00 °Brix and there were hybrid 14-3 （39.97 °Brix）, hybrid 8-16 （39.83 °Brix） and hybrid 7-3 （39.50 °Brix）. Based on the results of this study, Fl hybrids durian 1-15, 7-3, 8-16, 14-3 and 15-3 shall become successful candidates for recommendation to farmers for commercial production in Malaysia. However, further evaluations on fruit eating quality and stability of the hybrids should be completed prior to recommendation for commercialization.
基金supported by Shaanxi Normal University Academician Expert Workstation Foundation(1110010375)the National Key Laboratory of Protein and Plant Gene Research Open Project(ZD2021058)。
文摘Durian(Durio zibethinus)is a tropical fruit that has a unique flavor and aroma.It occupies a significant phylogenetic position within the Malvaceae family.Extant core-eudicot plants are reported to share seven ancestral karyotypes that have undergone reshuffling,resulting in an abundant genomic diversity.However,the ancestral karyotypes of the Malvaceae family,as well as the evolution trajectory leading to the28 chromosomes in durian,remain poorly understood.Here,we report the high-quality assembly of the durian genome with comprehensive comparative genomic analyses.By analyzing the collinear blocks between cacao and durian,we inferred 11 Malvaceae ancestral karyotypes.These blocks were present in a single-copy form in cacao and mainly in triplicates in durian,possibly resulting from a recent whole genome triplication(WGT)event that led to hexaploidization of the durian genome around 20(17–24)million years ago.A large proportion of the duplicated genes in durian,such as those involved in the lignin biosynthesis module for phenylpropane biosynthesis,are derived directly from whole genome duplication,which makes it an important force in reshaping its genomic architecture.Transcriptome studies have revealed that genes involved in feruloyl-Co A formations were highly preferentially expressed in fruit peels,indicating that the thorns produced on durian fruit may comprise guaiacyl and syringyl lignins.Among all the analyzed transcription factors(TFs),members of the heat shock factor family(HSF)were the most significantly upregulated under heat stress.All subfamilies of genes encoding heat shock proteins(HSPs)in the durian genome appear to have undergone expansion.The potential interactions between HSF Dzi05.397 and HSPs were examined and experimentally verified.Our study provides a high-quality durian genome and reveals the reshuffling mechanism of ancestral Malvaceae chromosomes to produce the durian genome.We also provide insights into the mechanism underlying lignin biosynthesis and heat stress tolerance.
