The increase in human population has led to imminent pressures to develop new edible proteins with decreased environmental footprints.The most promising approach involves the production of single cell protein(SCP)from...The increase in human population has led to imminent pressures to develop new edible proteins with decreased environmental footprints.The most promising approach involves the production of single cell protein(SCP)from yeasts,which have been utilized in a wide variety of foods for thousands of years.In this study,102 yeast strains isolated from traditional fermented pork(Nanx Wudl)were investigated for their potential as SCP producer for the first time.Based on preliminary screening,Saccharomyces cerevisiae Y70 and Candida parapsilosis H5Y13,both showing high protein content and excellent growth capability,were selected for further analysis via 4D-DIA proteomics technology.Proteomic analysis indicated that the oxidative metabolism pathways,including TCA cycle,oxidative phosphorylation and pentose phosphate pathway,may have a significant impact on global protein synthesis and production.This study provides useful information for selecting SCP-producing yeast from Chinese fermented meat products and contribute to a deeper understanding of the underlying metabolic mechanisms behind global protein synthesis in yeast.Furthermore,these findings also provide potential molecular targets for genetic engineering modifications in yeast,aimed at constructing highly efficient cell factories for protein production.展开更多
Background It is important to promote intestinal development and maturation of chicks for feed digestion and utilization,intestinal health,and disease resistance.This study aimed to investigate the effects of dietary ...Background It is important to promote intestinal development and maturation of chicks for feed digestion and utilization,intestinal health,and disease resistance.This study aimed to investigate the effects of dietary yeast cell wall polysaccharides(YCWP)addition on intestinal development and maturation of chickens and its potential action mechanism.Methods 180 one-day-old male Arbor Acres broilers were randomly assigned to three groups containing control(basal diets without any antibiotics or anticoccidial drug),bacitracin methylene disalicylate(BMD)-treated group(50 mg/kg)and YCWP-supplemented group(100 mg/kg).Results Compared with control group,in-feed antibiotic BMD continuous administration significantly decreased crypt depth(d 21)and villus height(d 42)along with mucosal maltase activity(d 42)in the ileum(P<0.05).Also,BMD markedly downregulated gene expression levels ofβ-catenin,lysozyme,occludin and FABP-2(d 21)and innate immune related genes CD83 and MHC-I mRNA levels(d 42,P<0.05),and decreased goblet cell counts in the ileum of chickens(d 21 and d 42,P<0.05).While,TLR-2,TLR-6 and iNOS mRNA abundances were notably upregulated by BMD treatment(d 42,P<0.05).Nevertheless,dietary YCWP addition significantly increased the ratio of villus height to crypt depth(d 21),villus surface area(d 21 and d 42),ileal alkaline phosphatase and maltase activities as well as goblet cell(d 21 and d 42)and IgA-producing plasma cell numbers as compared to BMD treatment(d 21,P<0.05).YCWP addition also upregulated gene expression levels of Lgr5,Wnt/β-catenin signaling pathway related gene(Wnt3,β-catenin,d 21;β-catenin,d 42),intestinal cells proliferation marker Ki-67 and barrier function related genes(occludin,d 21 and d 42,P<0.05).Moreover,YCWP significantly increased antigen presenting cell marker related genes(MHC-II,d 21;CD83 and MHC-I,d 42),TLR-1,TLR-2 and TLR-6 mRNA levels(d 21,P<0.05).Cecal microbiome analysis showed that YCWP addition obviously improved cecal microbial composition,as indicated by increasing relative abundance of Fournierella,Psychrobacter and Ruminiclostridium on d 21,and Alistipes and Lactobacillus on d 42,which were positively related with gut development and maturation related indexes(P<0.05).Conclusion Collectively, YCWP promoted yet antibiotic BMD delayed intestinal morphological and immunologicaldevelopment linked with modulating gut microbiome in chickens.展开更多
Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation a...Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation and minimal toxicity.Selenium-enriched yeast(SeY)is a well-established source,while selenium-enriched lactobacilli(SeL),a newer alternative,offers the added benefits of probiotics.This study examined the effects of SeY and SeL on egg quality,antioxidant capacity,Se deposition,and gut health in laying hens.After a two-week pre-treatment with a Sedeficient diet(SeD),450 Hy-Line Brown laying hens(30-week-old)were assigned into five dietary groups with six replicates of 15 hens each.The groups included a SeD,SeD supplemented with 1.5 mg Se/kg from SeY(SeY15),or 1.5,3.0,and 6.0 mg Se/kg from SeL(SeL15,SeL30,SeL60).The feeding trial lasted for 12 weeks.Results SeY15 and SeL15 improved the feed-to-egg ratio(P<0.05)in the latter stages.Haugh units were significantly increased(P<0.05)in the SeY15 and SeL30 groups,while darker yolk color(P<0.05)was observed in the SeY15,SeL15,and SeL60 groups.All Se-supplemented diets increased Se content in whole eggs,albumen,and yolk(P<0.05),while SeL groups showed a dose-dependent effect.Antioxidant enzyme activities increased,and MDA content decreased in the serum(P<0.05),with SeY15 showing the highest GSH-Px levels(P<0.05).SeL60 increased serum alkaline phosphatase and aspartate transaminase,and distorted the liver architecture(P<0.05).Se-diets reduced concentrations of reactive oxygen species(ROS)in the ileum and liver(P<0.05).SeL15 improved the ileal villus height-tocrypt depth ratio(P<0.05).SeY15 and/or SeL15 up-regulated TXNRD1 and SEPHS1 mRNA while down-regulating SCLY expression in the liver.SeY15 altered ileal microbiota by increasing both beneficial and pathogenic bacteria,whereas SeL15 predominantly boosted beneficial bacteria.Conclusion SeL integrates the antioxidant properties of organic Se with the probiotic benefits on gut health,resulting in a performance-enhancing effect comparable to that of SeY.However,high SeL level(6.0 mg Se/kg)compromised productivity and metabolic functions while enhancing Se deposition.展开更多
Background Using yeast culture as additives in ruminant feed prevents rumen microbial dysbiosis,enhances performance,and regulates rumen pH.The yeast culture used in this study was developed in-house,and has been show...Background Using yeast culture as additives in ruminant feed prevents rumen microbial dysbiosis,enhances performance,and regulates rumen pH.The yeast culture used in this study was developed in-house,and has been shown to promote rumen epithelial growth in several sheep trials.Changes in protein expression associated with the promotion of rumen epithelial development following the addition of yeast culture,along with the associated molecular mechanisms,remain unknown.We used 2045-day-old weaned lambs to investigate the specific proteins and molecular mechanisms involved in these processes.Half of the lambs were fed yeast culture,and the other half were used as controls.Results Yeast culture enhanced growth performance,facilitated rumen fermentation,and promoted rumen papilla development in weaned lambs.Proteomics data identified 4,831 proteins in the rumen epithelial tissue of lambs,comprising 87 upregulated and 425 downregulated proteins.Administration of yeast culture activated multiple molecular functions within rumen epithelial cells,including oxidative phosphorylation,glutathione metabolism,apoptosis,cell cycle,and vitamin digestion and absorption.The expression of proteins associated with cell cycle regulation increased,whereas those associated with apoptosis decreased.Administration of yeast culture also reduced the duration of the G0/G1 phase of rumen epithelial cells and accelerated the cell cycle.Furthermore,yeast culture showed increased cyclin D1,cyclin-dependent kinase(CDK)2,CDK4,CDK6,and cyclin E1 expressions and decreased cytochrome C(Cyto-c),Bcl-2-related X protein(Bax),cleaved caspase 3(C-caspase 3),caspase 3,and cleaved caspase 7(C-caspase 7)protein expressions.Yeast culture upregulated the insulin-like growth factor-1 receptor(IGF-1R)and insulin-like growth factor-binding protein 5(IGFBP-5)mRNA expressions in rumen epithelial cells.Conclusions Yeast culture facilitates rumen epithelial development by regulating the cell cycle and IGF-1 signaling and reducing the expression of proteins associated with apoptosis in rumen epithelial cells.The findings of this study provide novel insights into the molecular mechanisms through which yeast culture promotes rumen epithelial development in weaned lambs.展开更多
Low-density lipoprotein cholesterol(LDL-C)is the most causal risk factor for atherosclerotic cardiovascular disease(ASCVD).Red yeast rice(RYR)is a nutraceutical widely used as a lipid-lowering dietary supplement.The m...Low-density lipoprotein cholesterol(LDL-C)is the most causal risk factor for atherosclerotic cardiovascular disease(ASCVD).Red yeast rice(RYR)is a nutraceutical widely used as a lipid-lowering dietary supplement.The main cholesterol-lower agents in RYR are monacolins,particularly monacolin K,a weak reversible inhibitor of 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase,whose daily consumption(up to 10 mg/day)reduces LDL-C plasma levels up to 34%within 6-8 weeks when compared to placebo.The reduction in LDL-C is often accompanied by lower levels of plasma apolipoprotein B,total cholesterol,matrix metalloproteinases 2 and 9,high-sensitivity C-reactive protein,non-high-density lipoprotein cholesterol,and blood pressure.RYR has also demonstrated favorable reductions of up to 45%compared to placebo in the risk of ASCVD events in secondary prevention studies.The mechanism of action is similar to statins.When consumed appropriately,RYR is associated with only minimal side effects.Mild myalgia may be seen in patients who cannot tolerate low-dose statins.In individuals with no additional ASCVD risk factors,RYR is a safe and effective supplement in treating mild to moderate hyperlipidemia.展开更多
Alcoholic liver disease(ALD)is one of the major global public health problems.Yeast extract(YE),a product prepared from yeast,has been proven to have antioxidant and anti-inflammatory properties.However,the potential ...Alcoholic liver disease(ALD)is one of the major global public health problems.Yeast extract(YE),a product prepared from yeast,has been proven to have antioxidant and anti-inflammatory properties.However,the potential role of YE in the prevention of ALD remains unclear.The present study aimed to investigate the protective effects of YE on ALD and explore the underlying mechanism based on gut microbiota.The result showed that YE supplementation significantly ameliorated chronic alcohol exposure-induced liver injury in mice.In addition,YE counteracted alcohol-induced gut dysbiosis,intestinal barrier dysfunction,lipopolysaccharide(LPS)leakage-induced inflammatory response in the liver.Moreover,microbiota depletion by a broad-spectrum antibiotic was sufficient to block the protective effect of YE on ALD,indicating the contribution of gut dysbiosis modulation to the hepatoprotective role of YE.Furthermore,we demonstrated the causal relationship between gut microbiota and hepatoprotective effects of YE with the fecal microbiota transplantation(FMT)experiment.Compared with the ALD-FMT mice,gut dysbiosis,intestinal barrier dysfunction,LPS/TLR4 signaling pathway activation,and liver inflammatory response were significantly improved in the YE-FMT mice.Together,our findings highlight that dietary YE protects against ALD through gut dysbiosis correction.展开更多
Yeast-based models have become a powerful platform in pharmaceutical research,offering significant potential for producing complex drugs,vaccines,and therapeutic agents.While many current drugs were discovered before ...Yeast-based models have become a powerful platform in pharmaceutical research,offering significant potential for producing complex drugs,vaccines,and therapeutic agents.While many current drugs were discovered before fully understanding their molecular mechanisms,yeast systems now provide valuable insights for drug discovery and personalized medicine.Recent advancements in genetic engineering,metabolic engineering,and synthetic biology have improved the efficiency and scalability of yeast-based production systems,enabling more sustainable and cost-effective manufacturing processes.This paper reviews the latest developments in yeast-based technologies,focusing on their use as model organisms to study disease mechanisms,identify drug targets,and develop novel therapies.We highlight key platforms such as the yeast two-hybrid system,surface display technologies,and optimized expression systems.Additionally,we explore the future integration of yeast engineering with artificial intelligence(AI),machine learning(ML),and advanced genome editing technologies like CRISPR/Cas9,which are expected to accelerate drug discovery and enable personalized therapies.Furthermore,yeast-based systems are increasingly employed in largescale drug production,vaccine development,and therapeutic protein expression,offering promising applications in clinical and industrial settings.This paper discusses the practical implications of these systems and their potential to revolutionize drug development,paving the way for safer,more effective therapies.展开更多
In this study,we utilized gene knockout and overexpression techniques to generate brewer's yeast strains with either a deletion or overexpression of the fatty acyl-CoA oxidase(POX1)gene.The strains studied include...In this study,we utilized gene knockout and overexpression techniques to generate brewer's yeast strains with either a deletion or overexpression of the fatty acyl-CoA oxidase(POX1)gene.The strains studied included the parental strain,the POX1 deletion strain,and the POX1 overexpression strain.These strains were exposed to iso-αacid from hops at a concentration of 300 mg/L,leading to the induction of a viable but nonculturable(VBNC)state.Our results indicated that the silencing of the POX1 gene rendered brewer's yeast cells unable to withstand the high concentration of iso-αacid stress,ultimately leading to cell death.Conversely,the overexpression of POX1 accelerated the transition of yeast cells into the VBNC state compared to the parental strain.Furthermore,we evaluated the levels of reactive oxygen species(ROS),catalase(CAT)activity,superoxide dismutase(SOD)activity,glutathione reductase(GR)activity,and the m RNA expression of genes that regulate these enzymes(the stress-inducible yeast Mpv17(SYM1)gene,CTA1,SOD1,and glutathione reductase(GLR1)gene)in brewer's yeast cells at three distinct stages:normal,short-term stress,and VBNC states.Based on these findings,it can be inferred that the formation of the VBNC state in brewer's yeast is associated with the response to oxidative stress.展开更多
基金financial support of the National Natural Science Foundation of China(32102016)the Taishan Industrial Experts Program,Beijing Postdoctoral Research Foundation(2323ZZ122).
文摘The increase in human population has led to imminent pressures to develop new edible proteins with decreased environmental footprints.The most promising approach involves the production of single cell protein(SCP)from yeasts,which have been utilized in a wide variety of foods for thousands of years.In this study,102 yeast strains isolated from traditional fermented pork(Nanx Wudl)were investigated for their potential as SCP producer for the first time.Based on preliminary screening,Saccharomyces cerevisiae Y70 and Candida parapsilosis H5Y13,both showing high protein content and excellent growth capability,were selected for further analysis via 4D-DIA proteomics technology.Proteomic analysis indicated that the oxidative metabolism pathways,including TCA cycle,oxidative phosphorylation and pentose phosphate pathway,may have a significant impact on global protein synthesis and production.This study provides useful information for selecting SCP-producing yeast from Chinese fermented meat products and contribute to a deeper understanding of the underlying metabolic mechanisms behind global protein synthesis in yeast.Furthermore,these findings also provide potential molecular targets for genetic engineering modifications in yeast,aimed at constructing highly efficient cell factories for protein production.
基金funded by the National Natural Science Foundation of China(No.32172774)the Key Research and Development and Promotion of Special(Science and Technology)Project of Henan Province(No.242102110018).
文摘Background It is important to promote intestinal development and maturation of chicks for feed digestion and utilization,intestinal health,and disease resistance.This study aimed to investigate the effects of dietary yeast cell wall polysaccharides(YCWP)addition on intestinal development and maturation of chickens and its potential action mechanism.Methods 180 one-day-old male Arbor Acres broilers were randomly assigned to three groups containing control(basal diets without any antibiotics or anticoccidial drug),bacitracin methylene disalicylate(BMD)-treated group(50 mg/kg)and YCWP-supplemented group(100 mg/kg).Results Compared with control group,in-feed antibiotic BMD continuous administration significantly decreased crypt depth(d 21)and villus height(d 42)along with mucosal maltase activity(d 42)in the ileum(P<0.05).Also,BMD markedly downregulated gene expression levels ofβ-catenin,lysozyme,occludin and FABP-2(d 21)and innate immune related genes CD83 and MHC-I mRNA levels(d 42,P<0.05),and decreased goblet cell counts in the ileum of chickens(d 21 and d 42,P<0.05).While,TLR-2,TLR-6 and iNOS mRNA abundances were notably upregulated by BMD treatment(d 42,P<0.05).Nevertheless,dietary YCWP addition significantly increased the ratio of villus height to crypt depth(d 21),villus surface area(d 21 and d 42),ileal alkaline phosphatase and maltase activities as well as goblet cell(d 21 and d 42)and IgA-producing plasma cell numbers as compared to BMD treatment(d 21,P<0.05).YCWP addition also upregulated gene expression levels of Lgr5,Wnt/β-catenin signaling pathway related gene(Wnt3,β-catenin,d 21;β-catenin,d 42),intestinal cells proliferation marker Ki-67 and barrier function related genes(occludin,d 21 and d 42,P<0.05).Moreover,YCWP significantly increased antigen presenting cell marker related genes(MHC-II,d 21;CD83 and MHC-I,d 42),TLR-1,TLR-2 and TLR-6 mRNA levels(d 21,P<0.05).Cecal microbiome analysis showed that YCWP addition obviously improved cecal microbial composition,as indicated by increasing relative abundance of Fournierella,Psychrobacter and Ruminiclostridium on d 21,and Alistipes and Lactobacillus on d 42,which were positively related with gut development and maturation related indexes(P<0.05).Conclusion Collectively, YCWP promoted yet antibiotic BMD delayed intestinal morphological and immunologicaldevelopment linked with modulating gut microbiome in chickens.
基金supported by the National Natural Science Foundation of China(32302774)Beijing Innovation Consortium of Agriculture Research System(BAIC04)+1 种基金China Agriculture Research System(CARS-40)the Agricultural Science and Technology Innovation Program of the Feed Research Institute of the Chinese Academy of Agricultural Sciences(CAASIFR-ZDRW202402).
文摘Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation and minimal toxicity.Selenium-enriched yeast(SeY)is a well-established source,while selenium-enriched lactobacilli(SeL),a newer alternative,offers the added benefits of probiotics.This study examined the effects of SeY and SeL on egg quality,antioxidant capacity,Se deposition,and gut health in laying hens.After a two-week pre-treatment with a Sedeficient diet(SeD),450 Hy-Line Brown laying hens(30-week-old)were assigned into five dietary groups with six replicates of 15 hens each.The groups included a SeD,SeD supplemented with 1.5 mg Se/kg from SeY(SeY15),or 1.5,3.0,and 6.0 mg Se/kg from SeL(SeL15,SeL30,SeL60).The feeding trial lasted for 12 weeks.Results SeY15 and SeL15 improved the feed-to-egg ratio(P<0.05)in the latter stages.Haugh units were significantly increased(P<0.05)in the SeY15 and SeL30 groups,while darker yolk color(P<0.05)was observed in the SeY15,SeL15,and SeL60 groups.All Se-supplemented diets increased Se content in whole eggs,albumen,and yolk(P<0.05),while SeL groups showed a dose-dependent effect.Antioxidant enzyme activities increased,and MDA content decreased in the serum(P<0.05),with SeY15 showing the highest GSH-Px levels(P<0.05).SeL60 increased serum alkaline phosphatase and aspartate transaminase,and distorted the liver architecture(P<0.05).Se-diets reduced concentrations of reactive oxygen species(ROS)in the ileum and liver(P<0.05).SeL15 improved the ileal villus height-tocrypt depth ratio(P<0.05).SeY15 and/or SeL15 up-regulated TXNRD1 and SEPHS1 mRNA while down-regulating SCLY expression in the liver.SeY15 altered ileal microbiota by increasing both beneficial and pathogenic bacteria,whereas SeL15 predominantly boosted beneficial bacteria.Conclusion SeL integrates the antioxidant properties of organic Se with the probiotic benefits on gut health,resulting in a performance-enhancing effect comparable to that of SeY.However,high SeL level(6.0 mg Se/kg)compromised productivity and metabolic functions while enhancing Se deposition.
基金supported by the National Key Research and Development Program of China(2023YFE0100400)Science and Technology Project of Inner Mongolia Autonomous Region(2020GG0036)+2 种基金Basic Scientific Research Business Project of Universities directly under the Inner Mongolia Autonomous Region(BR22-11-17)National Center of Technology Innovation for Dairy(2023-JSGG-5)the Special Project for Improving the Research Ability of Young Teachers of Inner Mongolia Agricultural University(BR220133).
文摘Background Using yeast culture as additives in ruminant feed prevents rumen microbial dysbiosis,enhances performance,and regulates rumen pH.The yeast culture used in this study was developed in-house,and has been shown to promote rumen epithelial growth in several sheep trials.Changes in protein expression associated with the promotion of rumen epithelial development following the addition of yeast culture,along with the associated molecular mechanisms,remain unknown.We used 2045-day-old weaned lambs to investigate the specific proteins and molecular mechanisms involved in these processes.Half of the lambs were fed yeast culture,and the other half were used as controls.Results Yeast culture enhanced growth performance,facilitated rumen fermentation,and promoted rumen papilla development in weaned lambs.Proteomics data identified 4,831 proteins in the rumen epithelial tissue of lambs,comprising 87 upregulated and 425 downregulated proteins.Administration of yeast culture activated multiple molecular functions within rumen epithelial cells,including oxidative phosphorylation,glutathione metabolism,apoptosis,cell cycle,and vitamin digestion and absorption.The expression of proteins associated with cell cycle regulation increased,whereas those associated with apoptosis decreased.Administration of yeast culture also reduced the duration of the G0/G1 phase of rumen epithelial cells and accelerated the cell cycle.Furthermore,yeast culture showed increased cyclin D1,cyclin-dependent kinase(CDK)2,CDK4,CDK6,and cyclin E1 expressions and decreased cytochrome C(Cyto-c),Bcl-2-related X protein(Bax),cleaved caspase 3(C-caspase 3),caspase 3,and cleaved caspase 7(C-caspase 7)protein expressions.Yeast culture upregulated the insulin-like growth factor-1 receptor(IGF-1R)and insulin-like growth factor-binding protein 5(IGFBP-5)mRNA expressions in rumen epithelial cells.Conclusions Yeast culture facilitates rumen epithelial development by regulating the cell cycle and IGF-1 signaling and reducing the expression of proteins associated with apoptosis in rumen epithelial cells.The findings of this study provide novel insights into the molecular mechanisms through which yeast culture promotes rumen epithelial development in weaned lambs.
文摘Low-density lipoprotein cholesterol(LDL-C)is the most causal risk factor for atherosclerotic cardiovascular disease(ASCVD).Red yeast rice(RYR)is a nutraceutical widely used as a lipid-lowering dietary supplement.The main cholesterol-lower agents in RYR are monacolins,particularly monacolin K,a weak reversible inhibitor of 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase,whose daily consumption(up to 10 mg/day)reduces LDL-C plasma levels up to 34%within 6-8 weeks when compared to placebo.The reduction in LDL-C is often accompanied by lower levels of plasma apolipoprotein B,total cholesterol,matrix metalloproteinases 2 and 9,high-sensitivity C-reactive protein,non-high-density lipoprotein cholesterol,and blood pressure.RYR has also demonstrated favorable reductions of up to 45%compared to placebo in the risk of ASCVD events in secondary prevention studies.The mechanism of action is similar to statins.When consumed appropriately,RYR is associated with only minimal side effects.Mild myalgia may be seen in patients who cannot tolerate low-dose statins.In individuals with no additional ASCVD risk factors,RYR is a safe and effective supplement in treating mild to moderate hyperlipidemia.
基金supported by Angel Nutritech Nutrition Fund(AF2021003)。
文摘Alcoholic liver disease(ALD)is one of the major global public health problems.Yeast extract(YE),a product prepared from yeast,has been proven to have antioxidant and anti-inflammatory properties.However,the potential role of YE in the prevention of ALD remains unclear.The present study aimed to investigate the protective effects of YE on ALD and explore the underlying mechanism based on gut microbiota.The result showed that YE supplementation significantly ameliorated chronic alcohol exposure-induced liver injury in mice.In addition,YE counteracted alcohol-induced gut dysbiosis,intestinal barrier dysfunction,lipopolysaccharide(LPS)leakage-induced inflammatory response in the liver.Moreover,microbiota depletion by a broad-spectrum antibiotic was sufficient to block the protective effect of YE on ALD,indicating the contribution of gut dysbiosis modulation to the hepatoprotective role of YE.Furthermore,we demonstrated the causal relationship between gut microbiota and hepatoprotective effects of YE with the fecal microbiota transplantation(FMT)experiment.Compared with the ALD-FMT mice,gut dysbiosis,intestinal barrier dysfunction,LPS/TLR4 signaling pathway activation,and liver inflammatory response were significantly improved in the YE-FMT mice.Together,our findings highlight that dietary YE protects against ALD through gut dysbiosis correction.
基金funded by 2024 Scientific Research Project of Chongqing Medical and Pharmaceutical College(No.ygzrc2024101)Chongqing Education Commission Natural Science Foundation(No.KJQN202402821)+2 种基金Chongqing Shapingba District Science and Technology Bureau Project(No.2024071)2024 Chongqing Medical and Pharmaceutical College Innovation Research Group Project(No.ygz2024401)Chongqing Science and Health Joint Medical Research Project(No.2024SQKWLHMS051),respectively.
文摘Yeast-based models have become a powerful platform in pharmaceutical research,offering significant potential for producing complex drugs,vaccines,and therapeutic agents.While many current drugs were discovered before fully understanding their molecular mechanisms,yeast systems now provide valuable insights for drug discovery and personalized medicine.Recent advancements in genetic engineering,metabolic engineering,and synthetic biology have improved the efficiency and scalability of yeast-based production systems,enabling more sustainable and cost-effective manufacturing processes.This paper reviews the latest developments in yeast-based technologies,focusing on their use as model organisms to study disease mechanisms,identify drug targets,and develop novel therapies.We highlight key platforms such as the yeast two-hybrid system,surface display technologies,and optimized expression systems.Additionally,we explore the future integration of yeast engineering with artificial intelligence(AI),machine learning(ML),and advanced genome editing technologies like CRISPR/Cas9,which are expected to accelerate drug discovery and enable personalized therapies.Furthermore,yeast-based systems are increasingly employed in largescale drug production,vaccine development,and therapeutic protein expression,offering promising applications in clinical and industrial settings.This paper discusses the practical implications of these systems and their potential to revolutionize drug development,paving the way for safer,more effective therapies.
基金funded by National Natural Science Foundation of China(32272279)the Key R&D project of Shandong Province(2023CXPT007)the Key R&D project of Qingdao Science and Technology Plan(22-3-3-hygg-29-hy)。
文摘In this study,we utilized gene knockout and overexpression techniques to generate brewer's yeast strains with either a deletion or overexpression of the fatty acyl-CoA oxidase(POX1)gene.The strains studied included the parental strain,the POX1 deletion strain,and the POX1 overexpression strain.These strains were exposed to iso-αacid from hops at a concentration of 300 mg/L,leading to the induction of a viable but nonculturable(VBNC)state.Our results indicated that the silencing of the POX1 gene rendered brewer's yeast cells unable to withstand the high concentration of iso-αacid stress,ultimately leading to cell death.Conversely,the overexpression of POX1 accelerated the transition of yeast cells into the VBNC state compared to the parental strain.Furthermore,we evaluated the levels of reactive oxygen species(ROS),catalase(CAT)activity,superoxide dismutase(SOD)activity,glutathione reductase(GR)activity,and the m RNA expression of genes that regulate these enzymes(the stress-inducible yeast Mpv17(SYM1)gene,CTA1,SOD1,and glutathione reductase(GLR1)gene)in brewer's yeast cells at three distinct stages:normal,short-term stress,and VBNC states.Based on these findings,it can be inferred that the formation of the VBNC state in brewer's yeast is associated with the response to oxidative stress.
