The present study reports a successful attempt to produce single cell oil(SCO),heterogeneous base catalyst and yeast-based biodiesel from durian peel as a promising carbon feedstock by means of the waste-to-energy con...The present study reports a successful attempt to produce single cell oil(SCO),heterogeneous base catalyst and yeast-based biodiesel from durian peel as a promising carbon feedstock by means of the waste-to-energy concept.For this purpose,first,durian peel(DP)was hydrolyzed by dilute sulfuric acid to obtain xylose-rich DP hydrolysate(XDPH)and post-hydrolysis DP solid residue(DPS).Candida viswanathii PSY8,a newly isolated oleaginous yeast,showed high SCO accumulation(5.1±0.1 g/L)and SCO content(35.3±0.13%)on undetoxified XDPH medium.A novel heterogeneous base catalyst(DPS-K)prepared from DPS by wet impregnation technique with KOH,exhibited considerable catalytic activity to convert SCO-rich wet yeast of C.viswanathii PSY8 into yeast-based biodiesel(FAME)via direct transesterification with a maximum FAME yield of 94.3%under optimal conditions(6 wt%catalyst,10:1 methanol to wet yeast ratio,75℃,and 2 h).Moreover,most of the yeast-based biodiesel properties obtained from the FAME profiles were correlated well with the biodiesel standards limit of Thai,ASTM D6751 and EN 14214.Additionally,the energy output of FAME produced about 37.5 MJ/kg was estimated.Thus,this present finding demonstrated the favorable strategy for sustainable and eco-friendly production of new generation biodiesel.展开更多
The baker's yeast Saccharomyces(S.)cerevisiae is a single-celled eukaryotic model organism widely used in research on life sciences.Being a unicellular organism,S.cerevisiae has some evident limitations in applica...The baker's yeast Saccharomyces(S.)cerevisiae is a single-celled eukaryotic model organism widely used in research on life sciences.Being a unicellular organism,S.cerevisiae has some evident limitations in application to neuroscience.However,yeast prions are extensively studied and they are known to share some hallmarks with mammalian prion protein or other amyloidogenic proteins found in the pathogenesis of Alzheimer's,Parkinson's,or Huntington's diseases.Therefore,the yeast S.cerevisiae has been widely used for basic research on aggregation properties of proteins in cellulo and on their propagation.Recently,a yeast-based study revealed that some regions of mammalian prion protein and amyloidβ1–42 are capable of induction and propagation of yeast prions.It is one of the examples showing that evolutionarily distant organisms share common mechanisms underlying the structural conversion of prion proteins making yeast cells a useful system for studying mammalian prion protein.S.cerevisiae has also been used to design novel screening systems for anti-prion compounds from chemical libraries.Yeastbased assays are cheap in maintenance and safe for the researcher,making them a very good choice to perform preliminary screening before further characterization in systems engaging mammalian cells infected with prions.In this review,not only classical red/white colony assay but also yeast-based screening assays developed during last year are discussed.Computational analysis and research carried out using yeast prions force us to expect that prions are widely present in nature.Indeed,the last few years brought us several examples indicating that the mammalian prion protein is no more peculiar protein–it seems that a better understanding of prion proteins nature-wide may aid us with the treatment of prion diseases and other amyloid-related medical conditions.展开更多
This study evaluates the encapsulation of phytoactives from acerola(Malpighia emarginata)pomace extract(APE)into Saccharomyces cerevisiae cells via sonoprocessing coupled to spray drying(SPSD process).The effect of ac...This study evaluates the encapsulation of phytoactives from acerola(Malpighia emarginata)pomace extract(APE)into Saccharomyces cerevisiae cells via sonoprocessing coupled to spray drying(SPSD process).The effect of acoustic energy density(AED;0-333.3 W/L),APE pH(3-11),and spray drying inlet temperature(T;150-190℃)on the encapsulation efficiency(EE)and yield(EY)of phenolic compounds and anthocyanins were investigated through a Box-Behnken experi-mental design(BBD).Results demonstrated that maximized EEs(>63%)and EYs(>126.7 mg/100 g)were obtained using the highest AED(333.3 W/L)and alkaline APE(pH 11),while the optimal T was set at 170℃.Likewise,these process conditions also enhanced the antioxidant activity of biocapsules which positively correlated with the concentration of yeast encapsulated phytoactives by SPSD.Overall,our results demonstrate the versatility of SPSD protocol for manufacturing bioactive-enriched yeast biocapsules using complex mixtures of phytoactives.Further,it presents an innovative and sustainable roadmap for the management and repurposing of agri-food byproducts such as acerola pomace and other secondary streams of the food industry.展开更多
基金supported by the Program Management Unit for Human Resources&Institutional Development,Research and Innovation(Grant no.630000050102(15))The Genetic Conservation Project under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn,Khon Kaen University(Grant no.62000120010)Thailand.Additional support from the Research and Graduate Studies,Khon Kaen University,under the Research Program(Grant no.RP66-3-001)is appreciated.
文摘The present study reports a successful attempt to produce single cell oil(SCO),heterogeneous base catalyst and yeast-based biodiesel from durian peel as a promising carbon feedstock by means of the waste-to-energy concept.For this purpose,first,durian peel(DP)was hydrolyzed by dilute sulfuric acid to obtain xylose-rich DP hydrolysate(XDPH)and post-hydrolysis DP solid residue(DPS).Candida viswanathii PSY8,a newly isolated oleaginous yeast,showed high SCO accumulation(5.1±0.1 g/L)and SCO content(35.3±0.13%)on undetoxified XDPH medium.A novel heterogeneous base catalyst(DPS-K)prepared from DPS by wet impregnation technique with KOH,exhibited considerable catalytic activity to convert SCO-rich wet yeast of C.viswanathii PSY8 into yeast-based biodiesel(FAME)via direct transesterification with a maximum FAME yield of 94.3%under optimal conditions(6 wt%catalyst,10:1 methanol to wet yeast ratio,75℃,and 2 h).Moreover,most of the yeast-based biodiesel properties obtained from the FAME profiles were correlated well with the biodiesel standards limit of Thai,ASTM D6751 and EN 14214.Additionally,the energy output of FAME produced about 37.5 MJ/kg was estimated.Thus,this present finding demonstrated the favorable strategy for sustainable and eco-friendly production of new generation biodiesel.
基金funded by the Polish National Science Centre MINIATURA3,grant No.501/66 GR-6220(to TI)。
文摘The baker's yeast Saccharomyces(S.)cerevisiae is a single-celled eukaryotic model organism widely used in research on life sciences.Being a unicellular organism,S.cerevisiae has some evident limitations in application to neuroscience.However,yeast prions are extensively studied and they are known to share some hallmarks with mammalian prion protein or other amyloidogenic proteins found in the pathogenesis of Alzheimer's,Parkinson's,or Huntington's diseases.Therefore,the yeast S.cerevisiae has been widely used for basic research on aggregation properties of proteins in cellulo and on their propagation.Recently,a yeast-based study revealed that some regions of mammalian prion protein and amyloidβ1–42 are capable of induction and propagation of yeast prions.It is one of the examples showing that evolutionarily distant organisms share common mechanisms underlying the structural conversion of prion proteins making yeast cells a useful system for studying mammalian prion protein.S.cerevisiae has also been used to design novel screening systems for anti-prion compounds from chemical libraries.Yeastbased assays are cheap in maintenance and safe for the researcher,making them a very good choice to perform preliminary screening before further characterization in systems engaging mammalian cells infected with prions.In this review,not only classical red/white colony assay but also yeast-based screening assays developed during last year are discussed.Computational analysis and research carried out using yeast prions force us to expect that prions are widely present in nature.Indeed,the last few years brought us several examples indicating that the mammalian prion protein is no more peculiar protein–it seems that a better understanding of prion proteins nature-wide may aid us with the treatment of prion diseases and other amyloid-related medical conditions.
基金supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq),Grant number 140208/2022-4.
文摘This study evaluates the encapsulation of phytoactives from acerola(Malpighia emarginata)pomace extract(APE)into Saccharomyces cerevisiae cells via sonoprocessing coupled to spray drying(SPSD process).The effect of acoustic energy density(AED;0-333.3 W/L),APE pH(3-11),and spray drying inlet temperature(T;150-190℃)on the encapsulation efficiency(EE)and yield(EY)of phenolic compounds and anthocyanins were investigated through a Box-Behnken experi-mental design(BBD).Results demonstrated that maximized EEs(>63%)and EYs(>126.7 mg/100 g)were obtained using the highest AED(333.3 W/L)and alkaline APE(pH 11),while the optimal T was set at 170℃.Likewise,these process conditions also enhanced the antioxidant activity of biocapsules which positively correlated with the concentration of yeast encapsulated phytoactives by SPSD.Overall,our results demonstrate the versatility of SPSD protocol for manufacturing bioactive-enriched yeast biocapsules using complex mixtures of phytoactives.Further,it presents an innovative and sustainable roadmap for the management and repurposing of agri-food byproducts such as acerola pomace and other secondary streams of the food industry.
