Background The clinical significance of Y-H2AX in laryngeal squamous cell carcinoma （LSCC） has not yet been established. This study was performed to assess the expression of nuclear y-H2AX in benign and malignant la...Background The clinical significance of Y-H2AX in laryngeal squamous cell carcinoma （LSCC） has not yet been established. This study was performed to assess the expression of nuclear y-H2AX in benign and malignant laryngeal lesions and to assess its clinicopathological significance. Methods A total of 70 LSCC tumor-normal tissue paired samples were evaluated for y-H2AX expression using immunohistochemical staining. Their expression was correlated with different clinicopathological parameters. Results Nuclear Y-H2AX expression was frequently detected in LSCC tissues （P 〈0.001）. High nuclear Y-H2AX levels were not associated with any clinicopathological characteristics of LSCC （P 〉0.05）. Univariate Kaplan-Meier analysis showed that positive nuclear Y-H2AX expression was associated with a decreased overall survival （P=0.017）. Multivariate Cox regression analysis showed that nuclear Y-H2AX expression was an independent risk factor for overall survival. Conclusion The expression of nuclear Y-H2AX might be closely related to the prognosis of LSCC. Chin Med J 2014;127 （14）： 2664-2667展开更多
Objective： To analyze and discuss cell cycle＇s correlation of y-H2AX, so as to accumulate the data for the further studies of y-H2AX. Methods： MOLT-4 cells, and peripheral blood lymphocytes （PBLs）, with or withou...Objective： To analyze and discuss cell cycle＇s correlation of y-H2AX, so as to accumulate the data for the further studies of y-H2AX. Methods： MOLT-4 cells, and peripheral blood lymphocytes （PBLs）, with or without 48 h stimulation of phytohemagglutinin （PHA）, were irradiated by ultraviolet rays （UV rays）. Fluorescence-labeled y-H2AX antibody was used to detect γ-H2AX foci at the DNA double-strand breaks （DSBs） in chromatin, DNA damage was analyzed by flow cytometry, cell cycle and cell apoptosis were detected by sub-G1 peak method, the expression of γ-H2AX was detected by Western blot. Results： With the progression of time, sub-G1 peak emerged apparently in the DNA histograms, and the cells of apoptosis increased gradually; with the progression of time, the increase of γ-H2AX emerged and firstly raised, then decreased; PBLs with 48 h stimulation of PHA entered apparently cell cycle, cells of S and G2/M phase emerged, and PBLs without stimulation of PHA did not enter cell cycle; Western blot showed the increase of the expression of γ-H2AX, and the increase also firstly raised, then decreased. Conclusion： γ-H2AX expressed in the cells of stationary phase and proliferative phase, and with the progression of time, the increase of γ-H2AX firstly raised, and then decreased.展开更多
文摘Background The clinical significance of Y-H2AX in laryngeal squamous cell carcinoma （LSCC） has not yet been established. This study was performed to assess the expression of nuclear y-H2AX in benign and malignant laryngeal lesions and to assess its clinicopathological significance. Methods A total of 70 LSCC tumor-normal tissue paired samples were evaluated for y-H2AX expression using immunohistochemical staining. Their expression was correlated with different clinicopathological parameters. Results Nuclear Y-H2AX expression was frequently detected in LSCC tissues （P 〈0.001）. High nuclear Y-H2AX levels were not associated with any clinicopathological characteristics of LSCC （P 〉0.05）. Univariate Kaplan-Meier analysis showed that positive nuclear Y-H2AX expression was associated with a decreased overall survival （P=0.017）. Multivariate Cox regression analysis showed that nuclear Y-H2AX expression was an independent risk factor for overall survival. Conclusion The expression of nuclear Y-H2AX might be closely related to the prognosis of LSCC. Chin Med J 2014;127 （14）： 2664-2667
基金grants from the National Natural Sciences Foundation of China(No.30570908)Clinical Key Subject Foundation of Health Ministry of China"Cell Cycle Diagnosis and Analysisin ClinicalTumor(Ⅲ)"
文摘Objective： To analyze and discuss cell cycle＇s correlation of y-H2AX, so as to accumulate the data for the further studies of y-H2AX. Methods： MOLT-4 cells, and peripheral blood lymphocytes （PBLs）, with or without 48 h stimulation of phytohemagglutinin （PHA）, were irradiated by ultraviolet rays （UV rays）. Fluorescence-labeled y-H2AX antibody was used to detect γ-H2AX foci at the DNA double-strand breaks （DSBs） in chromatin, DNA damage was analyzed by flow cytometry, cell cycle and cell apoptosis were detected by sub-G1 peak method, the expression of γ-H2AX was detected by Western blot. Results： With the progression of time, sub-G1 peak emerged apparently in the DNA histograms, and the cells of apoptosis increased gradually; with the progression of time, the increase of γ-H2AX emerged and firstly raised, then decreased; PBLs with 48 h stimulation of PHA entered apparently cell cycle, cells of S and G2/M phase emerged, and PBLs without stimulation of PHA did not enter cell cycle; Western blot showed the increase of the expression of γ-H2AX, and the increase also firstly raised, then decreased. Conclusion： γ-H2AX expressed in the cells of stationary phase and proliferative phase, and with the progression of time, the increase of γ-H2AX firstly raised, and then decreased.
