This study optimized bioconversion process of mangosteen pericarp extract juice(MPEj)with a single-culture of Komagataeibacter xylinus TISTR 1061,a bacterial cellulose-producing strain and a co-culture with the probio...This study optimized bioconversion process of mangosteen pericarp extract juice(MPEj)with a single-culture of Komagataeibacter xylinus TISTR 1061,a bacterial cellulose-producing strain and a co-culture with the probiotic Lactiplantibacillus plantarum.K.xylinus TISTR 1061 was identified as the optimal bacterial cellulose-producing strain for fermenting MPEj.Optimal conditions were determined to be 20%(v/v)MPEj supplemented with 7%(w/v)sucrose and 0.2%(w/v)ammonium dihydrogen phosphate as carbon and nitrogen sources,respec-tively.The growth profile of single K.xylinus was not significantly different from those of the co-culture.They showed good adaptability and cohabited during fermentation without antagonistic effects.Dynamic changes in total titratable acidity(TTA),total soluble solids(TSS),and pH during fermentation were relatively similar for both single-and co-cultures.Post-fermented MPEj with co-culture exhibited significantly higher bioactive ac-tivities,particularly antioxidant properties.Significant variations in metabolite concentrations,including 2-ami-nobutyrate,valine,xylitol,propionate,isoleucine,glucose,and glycerol,were observed.These compounds exhibited significantly higher average concentrations after fermentation,highlighting their potential as bio-markers distinguishing pre-and post-fermentation MPEj.Bacterial cellulose(BC)from single culture exhibited greater thickness and weight,with a more complex and branched structure,while that from co-culture was infused with probiotic cells,showing less complexity allowing the BC from co-culture demonstrated low hardness and chewiness values.The properties of both bioconverted MPEj and BC obtained as observed offer potential for innovative functional drinks and/or dietary supplements with enhanced health benefits.展开更多
文摘This study optimized bioconversion process of mangosteen pericarp extract juice(MPEj)with a single-culture of Komagataeibacter xylinus TISTR 1061,a bacterial cellulose-producing strain and a co-culture with the probiotic Lactiplantibacillus plantarum.K.xylinus TISTR 1061 was identified as the optimal bacterial cellulose-producing strain for fermenting MPEj.Optimal conditions were determined to be 20%(v/v)MPEj supplemented with 7%(w/v)sucrose and 0.2%(w/v)ammonium dihydrogen phosphate as carbon and nitrogen sources,respec-tively.The growth profile of single K.xylinus was not significantly different from those of the co-culture.They showed good adaptability and cohabited during fermentation without antagonistic effects.Dynamic changes in total titratable acidity(TTA),total soluble solids(TSS),and pH during fermentation were relatively similar for both single-and co-cultures.Post-fermented MPEj with co-culture exhibited significantly higher bioactive ac-tivities,particularly antioxidant properties.Significant variations in metabolite concentrations,including 2-ami-nobutyrate,valine,xylitol,propionate,isoleucine,glucose,and glycerol,were observed.These compounds exhibited significantly higher average concentrations after fermentation,highlighting their potential as bio-markers distinguishing pre-and post-fermentation MPEj.Bacterial cellulose(BC)from single culture exhibited greater thickness and weight,with a more complex and branched structure,while that from co-culture was infused with probiotic cells,showing less complexity allowing the BC from co-culture demonstrated low hardness and chewiness values.The properties of both bioconverted MPEj and BC obtained as observed offer potential for innovative functional drinks and/or dietary supplements with enhanced health benefits.
