Wolfberry(Lycium barbarum L.)is a medicinal herb with anti-aging and neuroprotective effects.The purpose of this study was to explore the enhanced neuroprotective effects of red wolfberry water extract(RW),and brown w...Wolfberry(Lycium barbarum L.)is a medicinal herb with anti-aging and neuroprotective effects.The purpose of this study was to explore the enhanced neuroprotective effects of red wolfberry water extract(RW),and brown wolfberry water extract(BBW).The reduced percentage of reducing sugar in BBW was shown to be 62.8%,64.3%,39.8%,and 11.6%for glucose,fructose,maltose,and lactose compared to those of RW,as well as free amino acids.And the contents of total phenols and total flavonoids were increased by 62.0%and 51.0%.Melanoidins(1.00%)were first isolated from BBW.RW and BBW increased anti-stress abilities(oxidative stress:24.2%vs.35.7%,and heat stress:17.78%vs.57.57%)while decreasing the reactive oxygen species(ROS)levels in vivo.RW and BBW reduced the rate of paralysis and odor cognitive deficits.At the same time,RW and BBW reduced the number of fluorescent spots of Aβ::GFP.The RW and BBW activated autophagy by upregulating the gene levels(bec-1,lgg-1,lgg-2,unc-51,vps-34,atg-5,atg-18,and sqst-1).Additionally,BBW promoted the nuclear-cytoplasmic ratio of DAF-16::GFP and fluorescence intensity of SOD-3::GFP by 39.4 and 1.16 fold.This study laid a new insight for exploring the Maillard reaction in improving the anti-Alzheimer’s disease(AD)activity of wolfberry.展开更多
[Objective] The aim was to seek the appropriate utilization methods for imidacloprid pesticide in the production of Chinese wolfberry,as well as to provide scientific basis for the rational use of imidacloprid pestici...[Objective] The aim was to seek the appropriate utilization methods for imidacloprid pesticide in the production of Chinese wolfberry,as well as to provide scientific basis for the rational use of imidacloprid pesticide. [Method] The effect of different concentrations and spraying times of 10% WP of imidacloprid on the main secondary metabolites such as carotenoid and flavone,as well as the polysaccharide and total sugar content was studied in field. [Result] The effect of different concentrations of imidacloprid on the secondary metabolites and the content of polysaccharide of Chinese wolfberry was significant. After imidacloprid spraying,the contents of flavone,polysaccharide and total sugar on the 1st,7th and 14th d showed increasing trend,while the content of carotenoid showed decreasing trend. With the dilution times increasing,the content of the flavone,polysaccharide and total sugar showed increasing trend,while the content of carotenoid decreased,in which the highest content of the secondary metabolites and polysaccharide appeared in 1 800 times. [Conclusion] The dilution ratio of 1 800 times should be selected as the appropriate dosage of 10% WP of imidacloprid.展开更多
[Objective] The aim was to study the preservation of fresh Chinese wolfberry fruit via chitosan coating. [Method] Using Chinese wolfberry "Ningqi No. 1" as experimental material, the fresh fruits were coated...[Objective] The aim was to study the preservation of fresh Chinese wolfberry fruit via chitosan coating. [Method] Using Chinese wolfberry "Ningqi No. 1" as experimental material, the fresh fruits were coated by different concentrations of chitosan(0.75%, 1.00%, 1.25%, 1.50% and 1.75%) at room temperature to study the changes in weight loss rate, rate of rotten fruits rot, soluble solid content and vitamin C(Vc) content. [Result] Of all the treatments, the fruits coated with 1.25% chitosan showed lowest rate of rotten fruit and weight loss rate, and highest Vc and soluble solid content, presenting the optimal fresh-keeping effect. [Conclusion] Chitosan coating is helpful for the preservation of fresh Chinese wolfberry fruit at room temperature.展开更多
[Objective] This study aimed to conduct correspondence cluster analysis of the trace elements in Chinese wolfberry from Qinghai and Ningxia regions, and to investigate the relationship among the quality of the wolfber...[Objective] This study aimed to conduct correspondence cluster analysis of the trace elements in Chinese wolfberry from Qinghai and Ningxia regions, and to investigate the relationship among the quality of the wolfberry samples, the composition of trace elements and the sample sources. [Method] The determined contents of trace elements and ratios of zinc to copper （Zn/Cu） of wolfberry from 11 different producing areas of Qinghai and Ningxia regions were adopted to construct the raw measurement data matrix, to analyze the distribution characteristics of the trace ele- ments in wolfberry from Qinghai and Ningxia by using the corresponding cluster analysis method. [Result] The quality of wolfberry samples in 7hongning County, Zhongwei City, Pingluo County, Shizuishan City, Heicheng Town of Ningxia Hui Au-tonomous Region and Hehuang Valley, Golmud City of Qinghai Province is mainly related to the contents of Zn and Mn; Zn/Cu greatly affects the quality of Chinese wolfberry in Dulan County of Qinghai Province; Fe has great effect on the quality of Chinese wolfberry in Yinchuan City of Ningxia Hui Autonomous Region; Cu greatly affects the quality of Chinese wolfberry in Nuomuhong Village of Qinghai Province and a wolfberry research institute in Ningxia. [Conclusion] The relationship between the quality of wolfberry from different producing areas and the trace elements was investigated, which provides theoretical and practical basis for the cultivation, har- vesting, processing, and further development and utilization of Chinese wolfberry resources from different producing areas.展开更多
本研究拟利用LC-QTOF-MS非靶向代谢组学分析青海枸杞中黄酮代谢路径,阐明青藏高原独特气候因子的调控机制。采集青海3个产地的36份枸杞样本,并记录枸杞转色期(黄变前至黄变后)当地的气候参数(平均光照强度、平均温度、昼夜温差、积温、...本研究拟利用LC-QTOF-MS非靶向代谢组学分析青海枸杞中黄酮代谢路径,阐明青藏高原独特气候因子的调控机制。采集青海3个产地的36份枸杞样本,并记录枸杞转色期(黄变前至黄变后)当地的气候参数(平均光照强度、平均温度、昼夜温差、积温、空气湿度等)。非靶向代谢组学识别出青海枸杞中40种黄酮类和异黄酮类差异代谢物。利用火山图筛选出11个产地特异性差异代谢物,偏最小二乘判别分析枸杞产地的判别准确率为94.44%。京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析揭示黄酮类生物合成代谢途径中差异代谢物的代谢路径。Mental分析揭示平均光照强度和昼夜温差是青海枸杞产地特异性黄酮类物质生物合成的主要调控因子。极端梯度提升模型(eXtreme Gradient Boosting,XGBoost)对青海省枸杞产地的判别准确率达100%。本研究为了解青海枸杞的品质特性提供理论依据。展开更多
[Objective] The present study was undertaken to understand the salt tolerance of Chinese wolfberry seedling and lay the foundation for the cultivation of Chinese wolfberry. [Method] Using the potted seedlings of Lyciu...[Objective] The present study was undertaken to understand the salt tolerance of Chinese wolfberry seedling and lay the foundation for the cultivation of Chinese wolfberry. [Method] Using the potted seedlings of Lycium ruthenicum and Lycium barbarum as test materials, water treatment in NaCI solution, The leaf cell membrane permeability, malondialdehyde （MDA）, proline （Pro） and soluble sugar content of two varieties of Chinese wolfberry were measured by NaCI solution treatment, and their physiological responses were analyzed. [Result] NaCI had a significant effect on the physiological index of Chinese wolfberry, and the physiological indexes of the two varieties were consistent, but the degree of change was different. The cell membrane permeability, malondialdehyde （MDA）, and proline （pro） content of Lycium chincnse cv. Heiqi 1 were first increasing and then decreasing , the cell membrane permeability and proline （pro） content in the 45^th day reached the highest, and the malondialdehyde （MDA） content in the 30th day reached the highest value, the soluble sugar content showed a downward trend. The cell membrane permeability of Lycium chincnse cv. Ningqi 7 increased, the malondialdehyde （MDA） content was first decreasing and then increasing, and at the 30^th day reached the highest value; proline （pro） and soluble sugar content were first increasing and then decreasing, and at the 45th day reached the highest value. [Conclusion] The Lycium chincnse Heiqi 1 and Lycium chincnse Ningqi 7 both had a positive physiological response model to different NaCI concentration treatment, but there were differences on the physiological response model to different NaCI treatment between the two cultivars, in which Lycium chincnse Heiqi 1 had better adaptability than Lycium chincnse Ningqi 7 had.展开更多
This study is to investigate the potential of wolfberry water extracts for the treatment of Alzheimer’s disease(AD)and the relationship between their anti-AD property and the geographic locations in China.Total extra...This study is to investigate the potential of wolfberry water extracts for the treatment of Alzheimer’s disease(AD)and the relationship between their anti-AD property and the geographic locations in China.Total extracts of all wolfberry samples showed the potent to decrease self-induced Aβ1-42 aggregation and Cu2+-induced Aβ1-42 aggregation and the antioxidant capacity as well as the bioactivity for binding biometal.A transgenic drosophila model assay revealed that TE of S1 and S6(S1-TE and S6-TE)at 100μg/mL demonstrated the potent rescue effect on memory loss compared to the positive control memantine at 20μg/mL.Further study also revealed the polysaccharide extracts could contribute to their anti-AD property,and the anti-AD activity conspicuously depends on growing environment.Wolfberry samples S1 and S6 showed much more potent anti-AD property.Meanwhile this study demonstrated that the Chinese commonly consumed wolfberry in boiling water might have the beneficial function to prevent AD pathology.展开更多
Carbon（C） storage has received significant attention for its relevance to agricultural security and climate change. Afforestation can increase C storage in terrestrial ecosystems, and has been recognized as an impor...Carbon（C） storage has received significant attention for its relevance to agricultural security and climate change. Afforestation can increase C storage in terrestrial ecosystems, and has been recognized as an important measure to offset CO_2 emissions. In order to analyze the C benefits of planting wolfberry（Lycium barbarum L.） on the secondary saline lands in arid areas, we conducted a case study on the dynamics of biomass carbon（BC） storage and soil organic carbon（SOC） storage in different-aged wolfberry plantations（4-, 7-and 11-year-old） established on a secondary saline land as well as on the influence of wolfberry plantations on C storage in the plant-soil system in an arid irrigated area（Jingtai County） of Gansu Province, China. The C sequestration and its potential in the wolfberry plantations of Gansu Province were also evaluated. An intact secondary saline land was selected as control. Results show that wolfberry planting could decrease soil salinity, and increase BC, SOC and litter C storage of the secondary saline land significantly, especially in the first 4 years after planting. The aboveground and belowground BC storage values in the intact secondary saline land（control） accounted for only 1.0% and 1.2% of those in the wolfberry plantations, respectively. Compared to the intact secondary saline land, the SOC storage values in the 4-, 7-and 11-year-old wolfberry plantations increased by 36.4%, 37.3% and 43.3%, respectively, and the SOC storage in the wolfberry plantations occupied more than 92% of the ecosystem C storage. The average BC and SOC sequestration rates of the wolfberry plantations for the age group of 0–11 years were 0.73 and 3.30 Mg C/（hm^2·a）, respectively. There were no significant difference in BC and SOC storage between the 7-year-old and 11-year-old wolfberry plantations, which may be due in part to the large amounts of C offtakes in new branches and fruits. In Gansu Province, the C storage in the wolfberry plantations has reached up to 3.574 Tg in 2013, and the C sequestration potential of the existing wolfberry plantations was 0.134 Tg C/a. These results indicate that wolfberry planting is an ideal agricultural model to restore the degraded saline lands and increase the C sequestration capacity of agricultural lands in arid areas.展开更多
[Objective] The contact toxicity of the extracts from Hibiscus trionum L with different solvents against wolfberry aphis was studied.[Method] The drop method was used for the determination of the insecticidal activiti...[Objective] The contact toxicity of the extracts from Hibiscus trionum L with different solvents against wolfberry aphis was studied.[Method] The drop method was used for the determination of the insecticidal activities.[Result] 95% ethanol extract displayed the higher contact toxicity on wolfberry aphis and the corrected mortality rate of wolfberry aphids increased with the increasing of the concentration and the prolongation of treating time.The corrected mortality rate in 48 h reached 97.04% under the concentration of 50.00 g/L.The regression equation of virulence was y=1.791 0x＋3.750 2 and LC50 =4.986 7 g/L.The insecticidal activities of five extracts against wolfberry aphids were as follows：chloroformpetroleum etherethyl acetaten-butanol water.The corrected mortality rate of chloroform extract in 48 h was 97.04%.[Conclusion] The study provided a certain reference for further research and its development of H.trionum and its development of new types of botanical pesticide.展开更多
AIM: To investigate the anti-inflammatory properties of Lacto-Wolfberry (LWB), bothin vitro and using a mouse model of experimental colitis. METHODS: The effects of LWB on lipopolysaccharide (LPS)-induced reactive oxy...AIM: To investigate the anti-inflammatory properties of Lacto-Wolfberry (LWB), bothin vitro and using a mouse model of experimental colitis. METHODS: The effects of LWB on lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) and interleukin (IL)-6 secretion were assessed in a murine macrophage cell line. in vitro assessment also included characterizing the effects of LWB on the activation of NF-E2 related 2 pathway and inhibition of tumor necrosis factor-α (TNF-α)-induced nuclear factor-κB (NFκB) activation, utilizing reporter cell lines. Following the in vitro assessment, the anti-inflammatory efficacy of an oral intervention with LWB was tested in vivo using a preclinical model of intestinal inflammation. Multiple outcomes including body weight, intestinal histology, colonic cytokine levels and anti-oxidative measures were investigated.RESULTS: LWB reduced the LPS-mediated inductionof ROS production [+LPS vs 1% LWB + LPS, 1590 ± 188.5 relative luminescence units (RLU) vs 389 ± 5.9 RLU, P < 0.001]. LWB was more effective than wolfberry alone in reducing LPS-induced IL-6 secretion in vitro (wolfberry vs 0.5% LWB, 15% ± 7.8% vs 64% ± 5%, P < 0.001). In addition, LWB increased reporter gene expression via the anti-oxidant response element activation (wolfberry vs LWB, 73% ± 6.9% vs 148% ± 28.3%, P < 0.001) and inhibited the TNF-α-induced activation of the NF-κB pathway (milk vs LWB, 10% ± 6.7% vs 35% ± 3.3%, P < 0.05). Furthermore, oral supplementation with LWB resulted in a reduction of macroscopic (-LWB vs +LWB, 5.39 ± 0.61 vs 3.66 ± 0.59, P = 0.0445) and histological scores (-LWB vs +LWB, 5.44 ± 0.32 vs 3.66 ± 0.59, P = 0.0087) in colitic mice. These effects were associated with a significant decrease in levels of inflammatory cytokines such as IL-1β (-LWB vs +LWB, 570 ± 245 μg/L vs 89 ± 38 μg/L, P = 0.0106), keratinocyte-derived chemokine/growth regulated protein-α (-LWB vs +LWB, 184 ± 49 μg/Lvs 75 ± 20 μg/L,P = 0.0244), IL-6 (-LWBvs +LWB, 318 ± 99 μg/L vs 117 ± 18 μg/L, P = 0.0315) and other pro-inflammatory proteins such as cyclooxygenase-2 (-LWB vs +LWB, 0.95 ± 0.12 AU vs 0.36 ± 0.11 AU, P = 0.0036) and phosphorylated signal transducer and activator of transcription-3 (-LWB vs +LWB, 0.51 ± 0.15 AU vs 0.1 ± 0.04 AU, P = 0.057). Moreover, antioxidant biomarkers, including expression of gene encoding for the glutathione peroxidase, in the colon and the plasma anti-oxidant capacity were significantly increased by supplementation with LWB (-LWB vs +LWB, 1.2 ± 0.21 mmol/L vs 2.1 ± 0.19 mmol/L, P = 0.0095).CONCLUSION: These results demonstrate the antiinflammatory properties of LWB and suggest that the underlying mechanism is at least in part due to NF-κB inhibition and improved anti-oxidative capacity.展开更多
[Objectives] This study was conducted to use the feeding value of wolfberry branches and to provide a basis for the rational use of its active ingredients and nutrients and the development of new feed resources. [Meth...[Objectives] This study was conducted to use the feeding value of wolfberry branches and to provide a basis for the rational use of its active ingredients and nutrients and the development of new feed resources. [Methods] Wolfberry branches and whole-plant corn were used to make mixed silage of wolfberry branches, which replaced different proportions of whole-plant corn silage for feeding hybrid mutton sheep, and the feeding effect of the mixed silage of wolfberry branches on hybrid mutton sheep was analyzed, which provides a technical basis for rational utilization of mulberry resources to ruminants. A single factor feeding comparison experiment was carried out. 32 hybrid mutton sheep of 8 months old with conform gender, age, body weight, physiological state and feeding management level were selected and divided into two groups, 16 in each group. [Results] The gross output value of weight gain of the experimental group was 18.06 yuan, which was higher than the CK(12.97 yuan) by 5.09 yuan. [Conclusions] The addition of the mixed silage of wolfberry branches to the diet had a positive effect on the increase of the weight gain of the hybrid mutton sheep and the reduction of the feed conversion ratio compared with the single whole-plant corn silage.展开更多
基金supported by grants from Tianjin Science and Technology Plan Project(22ZYQYSY00030)Tianjin Municipal Health Technology Project(TJWJ2022XK04)+1 种基金Tianjin Key Medical Discipline(Specialty)Construction Project(TJYXZDXK-062B)Shandong Provincial Natural Science Foundation,China(ZR2023QC145).
文摘Wolfberry(Lycium barbarum L.)is a medicinal herb with anti-aging and neuroprotective effects.The purpose of this study was to explore the enhanced neuroprotective effects of red wolfberry water extract(RW),and brown wolfberry water extract(BBW).The reduced percentage of reducing sugar in BBW was shown to be 62.8%,64.3%,39.8%,and 11.6%for glucose,fructose,maltose,and lactose compared to those of RW,as well as free amino acids.And the contents of total phenols and total flavonoids were increased by 62.0%and 51.0%.Melanoidins(1.00%)were first isolated from BBW.RW and BBW increased anti-stress abilities(oxidative stress:24.2%vs.35.7%,and heat stress:17.78%vs.57.57%)while decreasing the reactive oxygen species(ROS)levels in vivo.RW and BBW reduced the rate of paralysis and odor cognitive deficits.At the same time,RW and BBW reduced the number of fluorescent spots of Aβ::GFP.The RW and BBW activated autophagy by upregulating the gene levels(bec-1,lgg-1,lgg-2,unc-51,vps-34,atg-5,atg-18,and sqst-1).Additionally,BBW promoted the nuclear-cytoplasmic ratio of DAF-16::GFP and fluorescence intensity of SOD-3::GFP by 39.4 and 1.16 fold.This study laid a new insight for exploring the Maillard reaction in improving the anti-Alzheimer’s disease(AD)activity of wolfberry.
基金Supported by National Natural Science Foundation of China (30860227)Ningxia Natural Science Foundation(NZ0603,NZ0639)~~
文摘[Objective] The aim was to seek the appropriate utilization methods for imidacloprid pesticide in the production of Chinese wolfberry,as well as to provide scientific basis for the rational use of imidacloprid pesticide. [Method] The effect of different concentrations and spraying times of 10% WP of imidacloprid on the main secondary metabolites such as carotenoid and flavone,as well as the polysaccharide and total sugar content was studied in field. [Result] The effect of different concentrations of imidacloprid on the secondary metabolites and the content of polysaccharide of Chinese wolfberry was significant. After imidacloprid spraying,the contents of flavone,polysaccharide and total sugar on the 1st,7th and 14th d showed increasing trend,while the content of carotenoid showed decreasing trend. With the dilution times increasing,the content of the flavone,polysaccharide and total sugar showed increasing trend,while the content of carotenoid decreased,in which the highest content of the secondary metabolites and polysaccharide appeared in 1 800 times. [Conclusion] The dilution ratio of 1 800 times should be selected as the appropriate dosage of 10% WP of imidacloprid.
基金Supported by the Scientific and Technological Project of Yinchuan City, Ningxia Province~~
文摘[Objective] The aim was to study the preservation of fresh Chinese wolfberry fruit via chitosan coating. [Method] Using Chinese wolfberry "Ningqi No. 1" as experimental material, the fresh fruits were coated by different concentrations of chitosan(0.75%, 1.00%, 1.25%, 1.50% and 1.75%) at room temperature to study the changes in weight loss rate, rate of rotten fruits rot, soluble solid content and vitamin C(Vc) content. [Result] Of all the treatments, the fruits coated with 1.25% chitosan showed lowest rate of rotten fruit and weight loss rate, and highest Vc and soluble solid content, presenting the optimal fresh-keeping effect. [Conclusion] Chitosan coating is helpful for the preservation of fresh Chinese wolfberry fruit at room temperature.
文摘[Objective] This study aimed to conduct correspondence cluster analysis of the trace elements in Chinese wolfberry from Qinghai and Ningxia regions, and to investigate the relationship among the quality of the wolfberry samples, the composition of trace elements and the sample sources. [Method] The determined contents of trace elements and ratios of zinc to copper （Zn/Cu） of wolfberry from 11 different producing areas of Qinghai and Ningxia regions were adopted to construct the raw measurement data matrix, to analyze the distribution characteristics of the trace ele- ments in wolfberry from Qinghai and Ningxia by using the corresponding cluster analysis method. [Result] The quality of wolfberry samples in 7hongning County, Zhongwei City, Pingluo County, Shizuishan City, Heicheng Town of Ningxia Hui Au-tonomous Region and Hehuang Valley, Golmud City of Qinghai Province is mainly related to the contents of Zn and Mn; Zn/Cu greatly affects the quality of Chinese wolfberry in Dulan County of Qinghai Province; Fe has great effect on the quality of Chinese wolfberry in Yinchuan City of Ningxia Hui Autonomous Region; Cu greatly affects the quality of Chinese wolfberry in Nuomuhong Village of Qinghai Province and a wolfberry research institute in Ningxia. [Conclusion] The relationship between the quality of wolfberry from different producing areas and the trace elements was investigated, which provides theoretical and practical basis for the cultivation, har- vesting, processing, and further development and utilization of Chinese wolfberry resources from different producing areas.
文摘本研究拟利用LC-QTOF-MS非靶向代谢组学分析青海枸杞中黄酮代谢路径,阐明青藏高原独特气候因子的调控机制。采集青海3个产地的36份枸杞样本,并记录枸杞转色期(黄变前至黄变后)当地的气候参数(平均光照强度、平均温度、昼夜温差、积温、空气湿度等)。非靶向代谢组学识别出青海枸杞中40种黄酮类和异黄酮类差异代谢物。利用火山图筛选出11个产地特异性差异代谢物,偏最小二乘判别分析枸杞产地的判别准确率为94.44%。京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析揭示黄酮类生物合成代谢途径中差异代谢物的代谢路径。Mental分析揭示平均光照强度和昼夜温差是青海枸杞产地特异性黄酮类物质生物合成的主要调控因子。极端梯度提升模型(eXtreme Gradient Boosting,XGBoost)对青海省枸杞产地的判别准确率达100%。本研究为了解青海枸杞的品质特性提供理论依据。
基金Demonstration and Promotion of Medlar Highly-efficient Cultivation Technology of Central Finance Forestry Science and Technology Promotion Project~~
文摘[Objective] The present study was undertaken to understand the salt tolerance of Chinese wolfberry seedling and lay the foundation for the cultivation of Chinese wolfberry. [Method] Using the potted seedlings of Lycium ruthenicum and Lycium barbarum as test materials, water treatment in NaCI solution, The leaf cell membrane permeability, malondialdehyde （MDA）, proline （Pro） and soluble sugar content of two varieties of Chinese wolfberry were measured by NaCI solution treatment, and their physiological responses were analyzed. [Result] NaCI had a significant effect on the physiological index of Chinese wolfberry, and the physiological indexes of the two varieties were consistent, but the degree of change was different. The cell membrane permeability, malondialdehyde （MDA）, and proline （pro） content of Lycium chincnse cv. Heiqi 1 were first increasing and then decreasing , the cell membrane permeability and proline （pro） content in the 45^th day reached the highest, and the malondialdehyde （MDA） content in the 30th day reached the highest value, the soluble sugar content showed a downward trend. The cell membrane permeability of Lycium chincnse cv. Ningqi 7 increased, the malondialdehyde （MDA） content was first decreasing and then increasing, and at the 30^th day reached the highest value; proline （pro） and soluble sugar content were first increasing and then decreasing, and at the 45th day reached the highest value. [Conclusion] The Lycium chincnse Heiqi 1 and Lycium chincnse Ningqi 7 both had a positive physiological response model to different NaCI concentration treatment, but there were differences on the physiological response model to different NaCI treatment between the two cultivars, in which Lycium chincnse Heiqi 1 had better adaptability than Lycium chincnse Ningqi 7 had.
基金National Natural Science Foundation of China(81673325 and 81711540311)。
文摘This study is to investigate the potential of wolfberry water extracts for the treatment of Alzheimer’s disease(AD)and the relationship between their anti-AD property and the geographic locations in China.Total extracts of all wolfberry samples showed the potent to decrease self-induced Aβ1-42 aggregation and Cu2+-induced Aβ1-42 aggregation and the antioxidant capacity as well as the bioactivity for binding biometal.A transgenic drosophila model assay revealed that TE of S1 and S6(S1-TE and S6-TE)at 100μg/mL demonstrated the potent rescue effect on memory loss compared to the positive control memantine at 20μg/mL.Further study also revealed the polysaccharide extracts could contribute to their anti-AD property,and the anti-AD activity conspicuously depends on growing environment.Wolfberry samples S1 and S6 showed much more potent anti-AD property.Meanwhile this study demonstrated that the Chinese commonly consumed wolfberry in boiling water might have the beneficial function to prevent AD pathology.
基金supported by the National Natural Science Foundation of China(31660232,41061030)the Carbon Benefits Project(G-4280-3)+1 种基金the Global Environmental Facility(GEF)Co-financed Project,the Foundation for Innovative Research Groups of Gansu Province(145RJIA335)the National Science and Technology Program for People's Livelihood(2013GS620202)
文摘Carbon（C） storage has received significant attention for its relevance to agricultural security and climate change. Afforestation can increase C storage in terrestrial ecosystems, and has been recognized as an important measure to offset CO_2 emissions. In order to analyze the C benefits of planting wolfberry（Lycium barbarum L.） on the secondary saline lands in arid areas, we conducted a case study on the dynamics of biomass carbon（BC） storage and soil organic carbon（SOC） storage in different-aged wolfberry plantations（4-, 7-and 11-year-old） established on a secondary saline land as well as on the influence of wolfberry plantations on C storage in the plant-soil system in an arid irrigated area（Jingtai County） of Gansu Province, China. The C sequestration and its potential in the wolfberry plantations of Gansu Province were also evaluated. An intact secondary saline land was selected as control. Results show that wolfberry planting could decrease soil salinity, and increase BC, SOC and litter C storage of the secondary saline land significantly, especially in the first 4 years after planting. The aboveground and belowground BC storage values in the intact secondary saline land（control） accounted for only 1.0% and 1.2% of those in the wolfberry plantations, respectively. Compared to the intact secondary saline land, the SOC storage values in the 4-, 7-and 11-year-old wolfberry plantations increased by 36.4%, 37.3% and 43.3%, respectively, and the SOC storage in the wolfberry plantations occupied more than 92% of the ecosystem C storage. The average BC and SOC sequestration rates of the wolfberry plantations for the age group of 0–11 years were 0.73 and 3.30 Mg C/（hm^2·a）, respectively. There were no significant difference in BC and SOC storage between the 7-year-old and 11-year-old wolfberry plantations, which may be due in part to the large amounts of C offtakes in new branches and fruits. In Gansu Province, the C storage in the wolfberry plantations has reached up to 3.574 Tg in 2013, and the C sequestration potential of the existing wolfberry plantations was 0.134 Tg C/a. These results indicate that wolfberry planting is an ideal agricultural model to restore the degraded saline lands and increase the C sequestration capacity of agricultural lands in arid areas.
基金Supported by National Natural Science Foundation of China (20565003)Natural Science Foundation of Ninxia Province (NZ0719)~~
文摘[Objective] The contact toxicity of the extracts from Hibiscus trionum L with different solvents against wolfberry aphis was studied.[Method] The drop method was used for the determination of the insecticidal activities.[Result] 95% ethanol extract displayed the higher contact toxicity on wolfberry aphis and the corrected mortality rate of wolfberry aphids increased with the increasing of the concentration and the prolongation of treating time.The corrected mortality rate in 48 h reached 97.04% under the concentration of 50.00 g/L.The regression equation of virulence was y=1.791 0x＋3.750 2 and LC50 =4.986 7 g/L.The insecticidal activities of five extracts against wolfberry aphids were as follows：chloroformpetroleum etherethyl acetaten-butanol water.The corrected mortality rate of chloroform extract in 48 h was 97.04%.[Conclusion] The study provided a certain reference for further research and its development of H.trionum and its development of new types of botanical pesticide.
文摘AIM: To investigate the anti-inflammatory properties of Lacto-Wolfberry (LWB), bothin vitro and using a mouse model of experimental colitis. METHODS: The effects of LWB on lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) and interleukin (IL)-6 secretion were assessed in a murine macrophage cell line. in vitro assessment also included characterizing the effects of LWB on the activation of NF-E2 related 2 pathway and inhibition of tumor necrosis factor-α (TNF-α)-induced nuclear factor-κB (NFκB) activation, utilizing reporter cell lines. Following the in vitro assessment, the anti-inflammatory efficacy of an oral intervention with LWB was tested in vivo using a preclinical model of intestinal inflammation. Multiple outcomes including body weight, intestinal histology, colonic cytokine levels and anti-oxidative measures were investigated.RESULTS: LWB reduced the LPS-mediated inductionof ROS production [+LPS vs 1% LWB + LPS, 1590 ± 188.5 relative luminescence units (RLU) vs 389 ± 5.9 RLU, P < 0.001]. LWB was more effective than wolfberry alone in reducing LPS-induced IL-6 secretion in vitro (wolfberry vs 0.5% LWB, 15% ± 7.8% vs 64% ± 5%, P < 0.001). In addition, LWB increased reporter gene expression via the anti-oxidant response element activation (wolfberry vs LWB, 73% ± 6.9% vs 148% ± 28.3%, P < 0.001) and inhibited the TNF-α-induced activation of the NF-κB pathway (milk vs LWB, 10% ± 6.7% vs 35% ± 3.3%, P < 0.05). Furthermore, oral supplementation with LWB resulted in a reduction of macroscopic (-LWB vs +LWB, 5.39 ± 0.61 vs 3.66 ± 0.59, P = 0.0445) and histological scores (-LWB vs +LWB, 5.44 ± 0.32 vs 3.66 ± 0.59, P = 0.0087) in colitic mice. These effects were associated with a significant decrease in levels of inflammatory cytokines such as IL-1β (-LWB vs +LWB, 570 ± 245 μg/L vs 89 ± 38 μg/L, P = 0.0106), keratinocyte-derived chemokine/growth regulated protein-α (-LWB vs +LWB, 184 ± 49 μg/Lvs 75 ± 20 μg/L,P = 0.0244), IL-6 (-LWBvs +LWB, 318 ± 99 μg/L vs 117 ± 18 μg/L, P = 0.0315) and other pro-inflammatory proteins such as cyclooxygenase-2 (-LWB vs +LWB, 0.95 ± 0.12 AU vs 0.36 ± 0.11 AU, P = 0.0036) and phosphorylated signal transducer and activator of transcription-3 (-LWB vs +LWB, 0.51 ± 0.15 AU vs 0.1 ± 0.04 AU, P = 0.057). Moreover, antioxidant biomarkers, including expression of gene encoding for the glutathione peroxidase, in the colon and the plasma anti-oxidant capacity were significantly increased by supplementation with LWB (-LWB vs +LWB, 1.2 ± 0.21 mmol/L vs 2.1 ± 0.19 mmol/L, P = 0.0095).CONCLUSION: These results demonstrate the antiinflammatory properties of LWB and suggest that the underlying mechanism is at least in part due to NF-κB inhibition and improved anti-oxidative capacity.
基金Supported by Ningxia Key R&D Program(2019BBF02016)Special Fund for Agricultural Science and Technology Independent Innovation of Ningxia Academy of Agriculture and Forestry Sciences
文摘[Objectives] This study was conducted to use the feeding value of wolfberry branches and to provide a basis for the rational use of its active ingredients and nutrients and the development of new feed resources. [Methods] Wolfberry branches and whole-plant corn were used to make mixed silage of wolfberry branches, which replaced different proportions of whole-plant corn silage for feeding hybrid mutton sheep, and the feeding effect of the mixed silage of wolfberry branches on hybrid mutton sheep was analyzed, which provides a technical basis for rational utilization of mulberry resources to ruminants. A single factor feeding comparison experiment was carried out. 32 hybrid mutton sheep of 8 months old with conform gender, age, body weight, physiological state and feeding management level were selected and divided into two groups, 16 in each group. [Results] The gross output value of weight gain of the experimental group was 18.06 yuan, which was higher than the CK(12.97 yuan) by 5.09 yuan. [Conclusions] The addition of the mixed silage of wolfberry branches to the diet had a positive effect on the increase of the weight gain of the hybrid mutton sheep and the reduction of the feed conversion ratio compared with the single whole-plant corn silage.
