Objective To elucidate the mechanism of Fuzheng Gankang Pill in treating combined allergic rhinitis and asthma syndrome(CARAS)with lung-spleen qi deficiency and wind-cold invading the lung syndrome using network pharm...Objective To elucidate the mechanism of Fuzheng Gankang Pill in treating combined allergic rhinitis and asthma syndrome(CARAS)with lung-spleen qi deficiency and wind-cold invading the lung syndrome using network pharmacology and molecular docking.MethodsThe active components and targets of the 13 herbs in Fuzheng Gankang Pill were retrieved from the TraditionalChinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and HERB.A“core herb-active component-target"network was constructed using Cytoscape to screen core components.CARAS disease targets were obtained from Genecards,NationalCenter for Biotechnology Information(NCBI),and Online Mendelian Inheritance in Man(OMIM).Targets related to the clinical phenotypes of CARAS with lung-spleen qi deficiency and wind-cold invading the lung syndrome were retrieved from the Traditional ChineseMedicineSyndrome Ontology and Multidimensional Quantitative Association Calculation Platform.The intersection of CARAS disease targets and syndromerelated targets yielded CARAS disease-syndrome targets.The intersection of Fuzheng Gankang Pill component-related targets and CARAS disease-syndrome targets provided“disease-syndrome-formula”intersection targets.These targets were uploaded to the STRING database for protein-protein interaction(PPI)network analysis,with topological analysis identifying key targets.Metascape was used for Gene Ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis.Molecular docking validation was performed using AutoDock Vina 1.1.2.Results The 13 core herbs of Fuzheng Gankang Pill contain a total of 200 active ingredients and 289 related targets.There are 2,412 disease targets for CARAS and 735 corresponding disease targets for the main and secondary symptoms of lung-spleen qi deficiency and wind-cold invading the lung.Through the Venn diagram,a total of 35 intersecting targets were obtained for Fuzheng Gankang Pill,CARAS,and the combination of lung-spleen qi deficiency and wind-cold invading the lung syndrome.Quercetin,Polygonatum sibiricum flavonoids,β-sitosterol,baicalein,kaempferol,etc.,are core components.PPI network analysis found that tumor necrosis factor(TNF),prostaglandin-endoperoxide synthase 2(PTGS2),interleukin(IL)-1β,IL-6,transforming growth factor beta 1(TGFβ1),BCL2,etc.,are the core targets for the compound to exert therapeutic effects.GO enrichment analysis showed that the 13 core drugs of Fuzheng Gankang Pill mainly participate in key biological processes such as positive regulation of protein modification,response to hormones,and negative regulation of cell population proliferation through protein kinases in areas such as membrane rafts,membrane microregions,plasma membrane protein complexes,and receptor com-plexes.KEGG enriched a total of 30 signaling pathways.Molecular docking shows that active ingredients such as quercetin and kaempferol bind stably toTNF(binding energy≤-9.0 kcal·mol^(-1))and PTGS2(≤-8.5kcal·mol^(-1)).Conclusion Fuzheng Gankang Pill may regulate biological processes such as cell apoptosis,tissue remodeling,inflammatory response,and immune response by acting on core targets such as TNF and PTGS2 through its core components quercetin,baicalein,β-sitosterol,baicalein,and kaempferol,thereby exerting therapeutic effects on CARAS with lung-spleen qi deficiency and wind-cold invading the lung syndrome.展开更多
Objective:To observe the clinical efficacy of modified painless suppurative moxibustion with wheat-grain sized moxa cones plus Western medication in treating cough variant asthma(CVA)due to wind-cold attacking the lun...Objective:To observe the clinical efficacy of modified painless suppurative moxibustion with wheat-grain sized moxa cones plus Western medication in treating cough variant asthma(CVA)due to wind-cold attacking the lung and its effects on pulmonary function,serum immunoglobulin(Ig)-E,hypersensitive C-reactive protein(hs-CRP),and interleukin(IL)-6.Methods:A total of 98 CVA patients were randomly divided into an observation group and a control group using the random number table method,with 49 cases in each group.The control group was treated with salmeterol xinafoate and fluticasone propionate powder for inhalation,and the observation group was treated with additional modified painless suppurative moxibustion with wheat-grain sized moxa cones.The treatment lasted for 8 weeks.The traditional Chinese medicine(TCM)symptom score of the patients in both groups was observed before treatment,after treatment,and at 1-month follow-up after treatment for its changes.The clinical efficacy after treatment and at 1-month follow-up was compared between the two groups.The pulmonary function[forced expiratory volume in the first second(FEV1),FEV1/forced vital capacity(FVC),and peak expiratory flow(PEF)]and serum IgE,hs-CRP,and IL-6 levels were compared between the two groups before and after treatment.The adverse reactions that occurred during the treatment in both groups were observed and recorded.Results:The total effective rate of the observation group after treatment and at follow-up was higher than that of the control group(P<0.05).The TCM symptom scores in both groups after treatment and at follow-up were lower compared to the baseline(P<0.05),and the scores in the observation group were lower than those in the control group(P<0.05).The FEV1,FEV1/FVC,and PEF after treatment in both groups were higher compared to the baseline(P<0.05),and the levels in the observation group were higher than those in the control group(P<0.05).The serum levels of IgE,IL-6,and hs-CRP after treatment in both groups were lower than the baseline(P<0.05),and the levels in the observation group were lower than those in the control group(P<0.05).There were no adverse reactions in either group during the course of the study.Conclusion:Modified painless suppurative moxibustion with wheat-grain sized moxa cones plus salmeterol xinafoate and fluticasone propionate powder for inhalation is effective in treating CVA due to wind-cold attacking the lung;it can relieve the clinical symptoms of the patients,improve their pulmonary function,and also reduce serum IgE,hs-CRP,and IL-6 levels.展开更多
基金supported by Special Project of Traditional Chinese Medicine Scientific Research in Henan Province(2023ZY1024,2022ZY1144)Special COVID-19 Research Project of Traditional Chinese Medicine in Henan Province(2022ZYFY08)+1 种基金Traditional Chinese Medicine Culture and Management Research Project in Henan Province(TCM2023005)Basic Scientific Research Business Fund Project of Henan Integrative Medicine Hospital(2304025,2304015)。
文摘Objective To elucidate the mechanism of Fuzheng Gankang Pill in treating combined allergic rhinitis and asthma syndrome(CARAS)with lung-spleen qi deficiency and wind-cold invading the lung syndrome using network pharmacology and molecular docking.MethodsThe active components and targets of the 13 herbs in Fuzheng Gankang Pill were retrieved from the TraditionalChinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and HERB.A“core herb-active component-target"network was constructed using Cytoscape to screen core components.CARAS disease targets were obtained from Genecards,NationalCenter for Biotechnology Information(NCBI),and Online Mendelian Inheritance in Man(OMIM).Targets related to the clinical phenotypes of CARAS with lung-spleen qi deficiency and wind-cold invading the lung syndrome were retrieved from the Traditional ChineseMedicineSyndrome Ontology and Multidimensional Quantitative Association Calculation Platform.The intersection of CARAS disease targets and syndromerelated targets yielded CARAS disease-syndrome targets.The intersection of Fuzheng Gankang Pill component-related targets and CARAS disease-syndrome targets provided“disease-syndrome-formula”intersection targets.These targets were uploaded to the STRING database for protein-protein interaction(PPI)network analysis,with topological analysis identifying key targets.Metascape was used for Gene Ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis.Molecular docking validation was performed using AutoDock Vina 1.1.2.Results The 13 core herbs of Fuzheng Gankang Pill contain a total of 200 active ingredients and 289 related targets.There are 2,412 disease targets for CARAS and 735 corresponding disease targets for the main and secondary symptoms of lung-spleen qi deficiency and wind-cold invading the lung.Through the Venn diagram,a total of 35 intersecting targets were obtained for Fuzheng Gankang Pill,CARAS,and the combination of lung-spleen qi deficiency and wind-cold invading the lung syndrome.Quercetin,Polygonatum sibiricum flavonoids,β-sitosterol,baicalein,kaempferol,etc.,are core components.PPI network analysis found that tumor necrosis factor(TNF),prostaglandin-endoperoxide synthase 2(PTGS2),interleukin(IL)-1β,IL-6,transforming growth factor beta 1(TGFβ1),BCL2,etc.,are the core targets for the compound to exert therapeutic effects.GO enrichment analysis showed that the 13 core drugs of Fuzheng Gankang Pill mainly participate in key biological processes such as positive regulation of protein modification,response to hormones,and negative regulation of cell population proliferation through protein kinases in areas such as membrane rafts,membrane microregions,plasma membrane protein complexes,and receptor com-plexes.KEGG enriched a total of 30 signaling pathways.Molecular docking shows that active ingredients such as quercetin and kaempferol bind stably toTNF(binding energy≤-9.0 kcal·mol^(-1))and PTGS2(≤-8.5kcal·mol^(-1)).Conclusion Fuzheng Gankang Pill may regulate biological processes such as cell apoptosis,tissue remodeling,inflammatory response,and immune response by acting on core targets such as TNF and PTGS2 through its core components quercetin,baicalein,β-sitosterol,baicalein,and kaempferol,thereby exerting therapeutic effects on CARAS with lung-spleen qi deficiency and wind-cold invading the lung syndrome.
文摘Objective:To observe the clinical efficacy of modified painless suppurative moxibustion with wheat-grain sized moxa cones plus Western medication in treating cough variant asthma(CVA)due to wind-cold attacking the lung and its effects on pulmonary function,serum immunoglobulin(Ig)-E,hypersensitive C-reactive protein(hs-CRP),and interleukin(IL)-6.Methods:A total of 98 CVA patients were randomly divided into an observation group and a control group using the random number table method,with 49 cases in each group.The control group was treated with salmeterol xinafoate and fluticasone propionate powder for inhalation,and the observation group was treated with additional modified painless suppurative moxibustion with wheat-grain sized moxa cones.The treatment lasted for 8 weeks.The traditional Chinese medicine(TCM)symptom score of the patients in both groups was observed before treatment,after treatment,and at 1-month follow-up after treatment for its changes.The clinical efficacy after treatment and at 1-month follow-up was compared between the two groups.The pulmonary function[forced expiratory volume in the first second(FEV1),FEV1/forced vital capacity(FVC),and peak expiratory flow(PEF)]and serum IgE,hs-CRP,and IL-6 levels were compared between the two groups before and after treatment.The adverse reactions that occurred during the treatment in both groups were observed and recorded.Results:The total effective rate of the observation group after treatment and at follow-up was higher than that of the control group(P<0.05).The TCM symptom scores in both groups after treatment and at follow-up were lower compared to the baseline(P<0.05),and the scores in the observation group were lower than those in the control group(P<0.05).The FEV1,FEV1/FVC,and PEF after treatment in both groups were higher compared to the baseline(P<0.05),and the levels in the observation group were higher than those in the control group(P<0.05).The serum levels of IgE,IL-6,and hs-CRP after treatment in both groups were lower than the baseline(P<0.05),and the levels in the observation group were lower than those in the control group(P<0.05).There were no adverse reactions in either group during the course of the study.Conclusion:Modified painless suppurative moxibustion with wheat-grain sized moxa cones plus salmeterol xinafoate and fluticasone propionate powder for inhalation is effective in treating CVA due to wind-cold attacking the lung;it can relieve the clinical symptoms of the patients,improve their pulmonary function,and also reduce serum IgE,hs-CRP,and IL-6 levels.
