Background:Forested landscapes are valuable sources of ecosystem services especially in areas dedicated to intense agricultural activities.Distance from forest margin is known to influence the wild bee community in th...Background:Forested landscapes are valuable sources of ecosystem services especially in areas dedicated to intense agricultural activities.Distance from forest margin is known to influence the wild bee community in the landscape surrounding forested patches.Yet little is known regarding how bee communities distribute themselves in landscapes that exhibit different forest successional states.Methods:We examined how land use type and distance from the forest edge affect the abundance and richness of the wild bee community across four forest successional states.Bees were collected in sites representing four stages of forest succession and analyzed using generalized linear mixed models with negative binomial distributions.Results:Wild bee diversity is reduced in forested environments that maintain dense stands of trees and high canopy cover.Additionally,distance from the forest edge was an important factor determining wild bee distribution in successional stages adjacent to forest edges.Furthermore,we found that bees maintain high specificity for distinct successional states and distances.Conclusions:Our results demonstrate how different successional stages can harbor different bee species and highlight the ability of microhabitats to act as reservoirs of bee diversity in and around forest margins.Furthermore,we found that maximizing successional patchiness across forested landscapes increases the amount of available habitat that can support a diverse suite of bees with different nesting biologies and behaviors.展开更多
Wild bees are ecologically vital but increasingly threatened by anthropogenic activities,leading to uncertain survival and health outcomes in urban environments.The gut microbiome contains features indicating host hea...Wild bees are ecologically vital but increasingly threatened by anthropogenic activities,leading to uncertain survival and health outcomes in urban environments.The gut microbiome contains features indicating host health and reflecting long-term evolutionary adaptation and acute reactions to real-time stressors.Moving beyond bacteria,we propose a comprehensive analysis integrating diet,bacteriome,virome,resistome,and their association to understand the survival status of urban lives better.We conducted a study on mason bees(Osmia excavata)across 10 urban agricultural sites in Suzhou,China,using shotgun gut metagenome sequencing for data derived from total gut DNA.Our findings revealed that most ingested pollen originated from Brassica crops and the unexpected garden tree Plantanus,indicating that floral resources at the 10 sites supported Osmia but with limited plant diversity.Varied city landscapes revealed site-specific flowers that all contributed to Osmia sustenance.The gut bacterial community,dominated by Gammaproteobacteria,showed remarkable structural stability across 8 sites but suggested perturbations at 2 sites.Antibiotic resistance gene profiles highly varied across 10 sites with prevalent unclassified drug classes,highlighting environmental threats to both bees and humans.The virome analysis identified honeybee pathogens,suggesting potential virus spillover.Many unknown bacteriophages were detected,some of which targeted the core gut bacteria,underscoring their role in maintaining gut homeostasis.These multifaceted metagenomic insights hold the potential to predict bee health and identify environmental threats,thereby guiding probiotic development and city management for effective bee conservation.展开更多
基金USDA National Institute of Food and Agriculture Hatch Project 1004515Foundation for Food and Agriculture Research Pollinator Health fund 549038.
文摘Background:Forested landscapes are valuable sources of ecosystem services especially in areas dedicated to intense agricultural activities.Distance from forest margin is known to influence the wild bee community in the landscape surrounding forested patches.Yet little is known regarding how bee communities distribute themselves in landscapes that exhibit different forest successional states.Methods:We examined how land use type and distance from the forest edge affect the abundance and richness of the wild bee community across four forest successional states.Bees were collected in sites representing four stages of forest succession and analyzed using generalized linear mixed models with negative binomial distributions.Results:Wild bee diversity is reduced in forested environments that maintain dense stands of trees and high canopy cover.Additionally,distance from the forest edge was an important factor determining wild bee distribution in successional stages adjacent to forest edges.Furthermore,we found that bees maintain high specificity for distinct successional states and distances.Conclusions:Our results demonstrate how different successional stages can harbor different bee species and highlight the ability of microhabitats to act as reservoirs of bee diversity in and around forest margins.Furthermore,we found that maximizing successional patchiness across forested landscapes increases the amount of available habitat that can support a diverse suite of bees with different nesting biologies and behaviors.
基金supported by the Research Development Fund(RDF-23-01-067)the Summer Undergraduate Research Fellowship(SURF)Program(SURF-2023-0079)from Xi'an Jiaotong-Liverpool University to M.T.
文摘Wild bees are ecologically vital but increasingly threatened by anthropogenic activities,leading to uncertain survival and health outcomes in urban environments.The gut microbiome contains features indicating host health and reflecting long-term evolutionary adaptation and acute reactions to real-time stressors.Moving beyond bacteria,we propose a comprehensive analysis integrating diet,bacteriome,virome,resistome,and their association to understand the survival status of urban lives better.We conducted a study on mason bees(Osmia excavata)across 10 urban agricultural sites in Suzhou,China,using shotgun gut metagenome sequencing for data derived from total gut DNA.Our findings revealed that most ingested pollen originated from Brassica crops and the unexpected garden tree Plantanus,indicating that floral resources at the 10 sites supported Osmia but with limited plant diversity.Varied city landscapes revealed site-specific flowers that all contributed to Osmia sustenance.The gut bacterial community,dominated by Gammaproteobacteria,showed remarkable structural stability across 8 sites but suggested perturbations at 2 sites.Antibiotic resistance gene profiles highly varied across 10 sites with prevalent unclassified drug classes,highlighting environmental threats to both bees and humans.The virome analysis identified honeybee pathogens,suggesting potential virus spillover.Many unknown bacteriophages were detected,some of which targeted the core gut bacteria,underscoring their role in maintaining gut homeostasis.These multifaceted metagenomic insights hold the potential to predict bee health and identify environmental threats,thereby guiding probiotic development and city management for effective bee conservation.
