Bone repair remains an important target in tissue engineering,making the development of bioactive scaffolds for effective bone defect repair a critical objective.In this study,β-tricalcium phosphate(β-TCP)scaffolds ...Bone repair remains an important target in tissue engineering,making the development of bioactive scaffolds for effective bone defect repair a critical objective.In this study,β-tricalcium phosphate(β-TCP)scaffolds incorporated with processed pyritum decoction(PPD)were fabricated using three-dimensional(3D)printing-assisted freeze-casting.The produced composite scaffolds were evaluated for their mechanical strength,physicochemical properties,biocompatibility,in vitro proangiogenic activity,and in vivo efficacy in repairing rabbit femoral defects.They not only demonstrated excellent physicochemical properties,enhanced mechanical strength,and good biosafety but also significantly promoted the proliferation,migration,and aggregation of pro-angiogenic human umbilical vein endothelial cells(HUVECs).In vivo studies revealed that all scaffold groups facilitated osteogenesis at the bone defect site,with theβ-TCP scaffolds loaded with PPD markedly enhancing the expression of neurogenic locus Notch homolog protein 1(Notch1),vascular endothelial growth factor(VEGF),bone morphogenetic protein-2(BMP-2),and osteopontin(OPN).Overall,the scaffolds developed in this study exhibited strong angiogenic and osteogenic capabilities both in vitro and in vivo.The incorporation of PPD notably promoted the angiogenic-osteogenic coupling,thereby accelerating bone repair,which suggests that PPD is a promising material for bone repair and that the PPD/β-TCP scaffolds hold great potential as a bone graft alternative.展开更多
Three-dimensional(3D)single molecule localization microscopy(SMLM)plays an important role in biomedical applications,but its data processing is very complicated.Deep learning is a potential tool to solve this problem....Three-dimensional(3D)single molecule localization microscopy(SMLM)plays an important role in biomedical applications,but its data processing is very complicated.Deep learning is a potential tool to solve this problem.As the state of art 3D super-resolution localization algorithm based on deep learning,FD-DeepLoc algorithm reported recently still has a gap with the expected goal of online image processing,even though it has greatly improved the data processing throughput.In this paper,a new algorithm Lite-FD-DeepLoc is developed on the basis of FD-DeepLoc algorithm to meet the online image processing requirements of 3D SMLM.This new algorithm uses the feature compression method to reduce the parameters of the model,and combines it with pipeline programming to accelerate the inference process of the deep learning model.The simulated data processing results show that the image processing speed of Lite-FD-DeepLoc is about twice as fast as that of FD-DeepLoc with a slight decrease in localization accuracy,which can realize real-time processing of 256×256 pixels size images.The results of biological experimental data processing imply that Lite-FD-DeepLoc can successfully analyze the data based on astigmatism and saddle point engineering,and the global resolution of the reconstructed image is equivalent to or even better than FD-DeepLoc algorithm.展开更多
Convectively unstable processes caused by dense water subsidence are common occurrences in high-latitude oceanic regions,and significantly modulate mass and heat transport and mixing processes in the ocean.An idealize...Convectively unstable processes caused by dense water subsidence are common occurrences in high-latitude oceanic regions,and significantly modulate mass and heat transport and mixing processes in the ocean.An idealized numerical experiment using the large eddy simulation method was conducted to analyze the three-dimensional flow field structure and the mechanism for dense water subsidence.Specifically,a negative salt flux is set at the sea surface,in which salt flux enters the sea surface to simulate the icing and salting-out phenomena that occur at high latitudes.Results show that the mean-state 3D flow field of dense water subsidence exhibits a hollow conical distribution.The horizontal flow field is characterized by a cyclonic vortex that driven primarily by the pressure gradient and influenced by the Coriolis effect.Moreover,the inverse vertical pressure gradient generated by this vortex inhibits the sinking of the plume,leading to its off-axis deflection and the development of an anticyclonic precession.In addition,the impact of rotation on the structure of a sinking plume within a stratified environment is discussed.Both horizontal vortex intensity and cone angle of the hollow cone flow field are increased with increasing rotation rate,resulting in a decrease in the plume’s maximum sinking depth.Variances in rotation direction cause the horizontal vortex and sinking plumes of dense water in the northern and southern hemispheres to rotate in opposite directions.展开更多
A series of Al-Ti-B master alloys were prepared by different preparation routes,and the TiB2 particles in the master alloys were extracted and analyzed.It is found that the forming process has significant influence on...A series of Al-Ti-B master alloys were prepared by different preparation routes,and the TiB2 particles in the master alloys were extracted and analyzed.It is found that the forming process has significant influence on the three-dimensional morphology of TiB2 particles.Different preparation routes result in different reaction forms,which accounts for the morphology variation of TiB2 particles.When the Al-Ti-B master alloy is prepared using ＂halide salt＂ route,TiB2 particles exhibit hexagonal platelet morphology and are independent with each other.In addition,the reaction temperature almost does not have influence on the morphology of TiB2 particles.However,TiB2 particles exhibit different morphologies at different reaction temperatures when the master alloys are prepared with Al-3B and Ti sponge.When the master alloy is prepared at 850 ℃,a kind of TiB2 particle agglomeration forms with a size larger than 5 μm.The TiB2 particles change to layered stacking morphology even dendritic morphology with the reaction temperature reaching up to 1200 ℃.展开更多
To study the influence of roll casting process parameters on temperature and thermal-stress fields for the AZ31 magnesium alloy sheets,three-dimensional geometric and 3D finite element models for roll casting were est...To study the influence of roll casting process parameters on temperature and thermal-stress fields for the AZ31 magnesium alloy sheets,three-dimensional geometric and 3D finite element models for roll casting were established based on the symmetry of roll casting by ANSYS software.Meshing method and smart-sizing algorithm were used to divide finite element mesh in ANSYS software.A series of researches on the temperature and stress distributions during solidification process with different process parameters were done by 3D finite element method.The temperatures of both the liquid-solid two-phase zone and liquid phase zone were elevated with increasing pouring temperature.With the heat transfer coefficient increasing,the two-phase region for liquid-solid becomes smaller.With the pouring temperature increasing and the increase of casting speed,the length of two-phase zone rises.The optimized of process parameters（casting speed 2 m/min,pouring temperature 640 ℃ and heat transfer coefficient 15 kW/（m2·℃） with the water pouring at roller exit was used to produce magnesium alloy AZ31 sheet,and equiaxed grains with the average grain size of 50 μm were achieved after roll casting.The simulation results give better understanding of the temperature variation in phase transformation zone and the formation mechanism of hot cracks in plates during roll casting and help to design the optimized process parameters of roll casting for Mg alloy.展开更多
A self-designed setup of modified sloping cooling/shearing process was made to prepare the semisolid Al-3wt%Mg alloy. A three-dimensional simulation model was established for the analysis of preparing the semisolid Al...A self-designed setup of modified sloping cooling/shearing process was made to prepare the semisolid Al-3wt%Mg alloy. A three-dimensional simulation model was established for the analysis of preparing the semisolid Al-3wt%Mg alloy. Through simulation and experiment, it is shown that the sloping angle of the plate greatly affects temperature and velocity distributions, and the temperature and velocity of the alloy at the exit of the sloping plate increase with the increase of the sloping angle. The alloy temperature decreases linearly from the pouring mouth to the exit. The alloy temperature at the exit increases obviously with the increase of pouring temperature. To prepare the semisolid Al-3wt%Mg alloy with good quality, the sloping angle θ=45° is reasonable, and the pouring temperature is suggested to be designed above 650-660℃ but under 700℃.展开更多
Some techniques such as die surface description, contact judgement algorithm and remeshing are proposed to improve the robustness of the numerical solution. Based on these techniques, a three-dimensional rigid-plastic...Some techniques such as die surface description, contact judgement algorithm and remeshing are proposed to improve the robustness of the numerical solution. Based on these techniques, a three-dimensional rigid-plastic FEM code has been developed. Isothermal forging process of a cylindrical housing has been simulated. The simulation results show that the given techniques and the FEM code are reasonable and feasible for three-dimensional bulk forming processes.展开更多
The fabrication process dependent effects on single event effects （SEEs） are investigated in a commercial silicon- germanium heterojunction bipolar transistor （SiGe HBT） using three-dimensional （3D） TCAD simulat...The fabrication process dependent effects on single event effects （SEEs） are investigated in a commercial silicon- germanium heterojunction bipolar transistor （SiGe HBT） using three-dimensional （3D） TCAD simulations. The influences of device structure and doping concentration on SEEs are discussed via analysis of current transient and charge collection induced by ions strike. The results show that the SEEs representation of current transient is different from representation of the charge collection for the same process parameters. To be specific, the area of C/S junction is the key parameter that affects charge collection of SEE. Both current transient and charge collection are dependent on the doping of collector and substrate. The base doping slightly influences transient currents of base, emitter, and collector terminals. However, the SEEs of SiGe HBT are hardly affected by the doping of epitaxial base and the content of Ge.展开更多
A method of constructing three-dimensional process model for the punching cartridge cases is presented based on DEFORM simulation analysis. Using DEFORM software,the finite element simulation models for the punching a...A method of constructing three-dimensional process model for the punching cartridge cases is presented based on DEFORM simulation analysis. Using DEFORM software,the finite element simulation models for the punching and forming process of cartridge cases are established,and the corresponding simulation result model of each intermediate procedure is obtained by continuously performing the forming process simulation. The simulation model cannot annotate size and process information due to poor interface between DEFORM software and CAD software. Thus,a 3D annotation module is developed with secondary development technology of UG NX software. Consequently,the final process model with dimension and process information is obtained. Then,with the current 3D process management system,the 3D punching and forming process design of cartridge cases can be completed further. An example is also provided to illustrate that the relative error between the simulation process model and the physical model is less than 2%,which proves the validity and reliability of the proposed method in this study.展开更多
The traditional fuzzy logic system （FLS） can only model and control the process in two-dimensional nature. Many of real-world systems are of multidimensional features, such as, thermal and fluid processes with spati...The traditional fuzzy logic system （FLS） can only model and control the process in two-dimensional nature. Many of real-world systems are of multidimensional features, such as, thermal and fluid processes with spatiotemporal dynamics, biological systems, or decision-making processes that contain stochastic and imprecise uncertainties. These types of systems are difficult for the traditional FLS to model and control because they require a third dimension for spatial or probabilistic information. The type-2 fuzzy set provides the possibility to develop a three-dimensional fuzzy logic system for modeling and controlling these processes in three-dimensional nature.展开更多
True-triaxial compression tests were carried out on cubic granite samples with a circular through hole using a true-triaxial testing system to investigate the influence of saturated water content(SWC) on the failure p...True-triaxial compression tests were carried out on cubic granite samples with a circular through hole using a true-triaxial testing system to investigate the influence of saturated water content(SWC) on the failure process and characteristics of a circular tunnel of surrounding rocks. The spalling failure under SWC can be divided into four periods: calm period, buckling deformation period, period of rock fragment gradual buckling and exfoliation, and period of formation of symmetrical V-shaped notches. When the horizontal axial and vertical stresses were constant, the spalling failure severity was reduced with the increase in lateral stress. Under natural water content, a strong rockburst with dynamic failure characteristics occurred on the circular hole sidewall. Under SWC, the failure severity was reduced and the circular hole sidewall experienced spalling failure, exhibiting progressive static failure characteristics.Therefore, water can reduce the failure severity of surrounding rocks in deep underground engineering, which has a certain guiding significance for the prevention and control of rockbursts.展开更多
To shorten the preparation process of semi-solid billets,semi-solid billets of 2A14 aluminum alloy were prepared by wrought aluminum directly semi-solid isothermal treatment(WADSSIT)process.Three-dimension(3D)combined...To shorten the preparation process of semi-solid billets,semi-solid billets of 2A14 aluminum alloy were prepared by wrought aluminum directly semi-solid isothermal treatment(WADSSIT)process.Three-dimension(3D)combined microstructure evolution,namely transverse direction(TD)surface,rolling direction(RD)surface,and normal direction(ND)surface,was studied.Effects of temperature and holding time on average grain size and average shape factor were investigated.The results showed that the optimum conditions for preparation of 2A14 semi-solid billets by this process were 615℃ and 20 min(average grain size of 124μm and shape factor of 0.81).Electron backscatter diffraction(EBSD)observations indicated that the microstructure was completely recrystallized when it was heated to 600℃.Grain size was increased with the increase of temperature and grew up slowly with the holding time prolonging.Roundness was increased with increase of holding time but was not sensitive to temperature.展开更多
The extremely high peak intensity associated with ultrashort pulse width of femtosecond(fs)lasers enabled inducing nonlinear multiphoton absorption in materials that are transparent to the laser wavelength.More import...The extremely high peak intensity associated with ultrashort pulse width of femtosecond(fs)lasers enabled inducing nonlinear multiphoton absorption in materials that are transparent to the laser wavelength.More importantly,focusing the fs laser beam inside the transparent materials confined the nonlinear interaction to within the focal volume only,realizing three-dimensional(3D)micro/nanofabrication.This 3D capability offers three different processing schemes for use in fabrication:undeformative,subtractive,and additive.Furthermore,a hybrid approach of different schemes can create much more complex 3D structures and thereby promises to enhance the functionality of the structures created.Thus,hybrid fs laser 3D microprocessing opens a new door for material processing.This paper comprehensively reviews different types of hybrid fs laser 3D micro/nanoprocessing for diverse applications including fabrication of functional micro/nanodevices.展开更多
To address the problem of multi-missile cooperative interception against maneuvering targets at a prespecified impact time and desired Line-of-Sight(LOS)angles in ThreeDimensional(3D)space,this paper proposes a 3D lea...To address the problem of multi-missile cooperative interception against maneuvering targets at a prespecified impact time and desired Line-of-Sight(LOS)angles in ThreeDimensional(3D)space,this paper proposes a 3D leader-following cooperative interception guidance law.First,in the LOS direction of the leader,an impact time-controlled guidance law is derived based on the fixed-time stability theory,which enables the leader to complete the interception task at a prespecified impact time.Next,in the LOS direction of the followers,by introducing a time consensus tracking error function,a fixed-time consensus tracking guidance law is investigated to guarantee the consensus tracking convergence of the time-to-go.Then,in the direction normal to the LOS,by combining the designed global integral sliding mode surface and the second-order Sliding Mode Control(SMC)theory,an innovative 3D LOS-angle-constrained interception guidance law is developed,which eliminates the reaching phase in the traditional sliding mode guidance laws and effectively saves energy consumption.Moreover,it effectively suppresses the chattering phenomenon while avoiding the singularity issue,and compensates for unknown interference caused by target maneuvering online,making it convenient for practical engineering applications.Finally,theoretical proof analysis and multiple sets of numerical simulation results verify the effectiveness,superiority,and robustness of the investigated guidance law.展开更多
Air separators provide safe, clean, and appropriate air flow to engines and are widely used in vehicles with large engines such as ships and submarines. In this operational study, the separation process in a Ranque-Hi...Air separators provide safe, clean, and appropriate air flow to engines and are widely used in vehicles with large engines such as ships and submarines. In this operational study, the separation process in a Ranque-Hilsch vortex tube cleaning （cooling） system is investigated to analyze the impact of the operating gas type on the vortex tube performance; the operating gases used are air, nitrogen, oxygen, carbon dioxide and nitrogen dioxide. The computational fluid dynamic model used is equipped with a three-dimensional structure, and the steady-state condition is applied during computations. The standard k-c turbulence model is employed to resolve nonlinear flow equations, and various key parameters, such as hot and cold exhaust thermal drops, and power separation rates, are described numerically. The results show that nitrogen dioxide creates the greatest separation power out of all gases tested, and the numerical results are validated by good agreement with available experimental data. In addition, a comparison is made between the use of two different boundary conditions, the pressure-far-field and the pressure-outlet, when analyzing complex turbulent flows in the air separators. Results present a comprehensive and practical solution for use in future numerical studies.展开更多
In this context, recent developments in the coupled three-dimensional(3 D) hydro-mechanical(HM)simulation tool TOUGH-RBSN are presented. This tool is used to model hydraulic fracture in geological media, as observed i...In this context, recent developments in the coupled three-dimensional(3 D) hydro-mechanical(HM)simulation tool TOUGH-RBSN are presented. This tool is used to model hydraulic fracture in geological media, as observed in laboratory-scale tests. The TOUGH-RBSN simulator is based on the effective linking of two numerical methods: TOUGH2, a finite volume method for simulating mass transport within a permeable medium; and a lattice model based on the rigid-body-spring network(RBSN) concept. The method relies on a Voronoi-based discretization technique that can represent fracture development within a permeable rock matrix. The simulator provides two-way coupling of HM processes, including fluid pressure-induced fracture and fracture-assisted flow. We first present the basic capabilities of the modeling approach using two example applications, i.e. permeability evolution under compression deformation, and analyses of a static fracturing simulation. Thereafter, the model is used to simulate laboratory tests of hydraulic fracturing in granite. In most respects, the simulation results meet expectations with respect to permeability evolution and fracturing patterns. It can be seen that the evolution of injection pressure associated with the simulated fracture developments is strongly affected by fluid viscosity.展开更多
A meshless approach, called the rigid-plastic reproducing kernel particle method （RKPM）, is presented for three-dimensional （3D） bulk metal forming simulation. The approach is a combination of RKPM with the flow t...A meshless approach, called the rigid-plastic reproducing kernel particle method （RKPM）, is presented for three-dimensional （3D） bulk metal forming simulation. The approach is a combination of RKPM with the flow theory of 3D rigid-plastic mechanics. For the treatments of essential boundary conditions and incompressibility constraint, the boundary singular kernel method and the modified penalty method are utilized, respectively. The arc-tangential friction model is employed to treat the contact conditions. The compression of rectangular blocks, a typical 3D upsetting operation, is analyzed for different friction conditions and the numerical results are compared with those obtained using commercial rigid-plastic FEM （finite element method） software Deform^3D. As results show, when handling 3D plastic deformations, the proposed approach eliminates the need of expensive meshing and remeshing procedures which are unavoidable in conventional FEM and can provide results that are in good agreement with finite element predictions.展开更多
Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma ...Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma pathobiology.Conventional cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under discussion.In vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture.However,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)characteristics.Therefore,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between them.Given the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development.In this review,we first provide a general overview of 3D cell cultures compared to 2D cell cultures.We then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO methodologies.Finally,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma.展开更多
The three-dimensional particle electrode system exhibits significant potential for application in the treatment of wastewater.Nonetheless,the advancement of effective granular electrodes characterized by elevated cata...The three-dimensional particle electrode system exhibits significant potential for application in the treatment of wastewater.Nonetheless,the advancement of effective granular electrodes characterized by elevated catalytic activity and minimal energy consumption continues to pose a significant challenge.In this research,Fluorine-doped copper-carbon(F/Cu-GAC)particle electrodes were effectively synthesized through an impregnationcalcination technique,utilizing granular activated carbon as the carrier and fluorinedoped modified copper oxides as the catalytic agents.The particle electrodes were subsequently utilized to promote the degradation of 2,4,6-trichlorophenol(2,4,6-TCP)in a threedimensional electrocatalytic reactor(3DER).The F/Cu-GAC particle electrodes were polarized under the action of electric field,which promoted the heterogeneous Fenton-like reaction in which H2O2 generated by two-electron oxygen reduction reaction(2e-ORR)of O_(2) was catalytically decomposed to·OH.The 3DER equipped with F/Cu-GAC particle electrodes showed 100%removal of 2,4,6-TCP and 79.24%removal of TOC with a specific energy consumption(EC)of approximately 0.019 kWh/g·COD after 2 h of operation.The F/Cu-GAC particle electrodes exhibited an overpotential of 0.38 V and an electrochemically active surface area(ECSA)of 715 cm^(2),as determined through linear sweep voltammetry(LSV)and cyclic voltammetry(CV)assessments.These findings suggest a high level of electrocatalytic performance.Furthermore,the catalytic mechanism of the 3DER equipped with F/Cu-GAC particle electrodes was elucidated through the application of X-ray photoelectron spectroscopy(XPS),electron spin resonance(ESR),and active species capture experiments.This investigation offers a novel approach for the effective degradation of 2,4,6-TCP.展开更多
Gas hydrate drilling expeditions in the Pearl River Mouth Basin,South China Sea,have identified concentrated gas hydrates with variable thickness.Moreover,free gas and the coexistence of gas hydrate and free gas have ...Gas hydrate drilling expeditions in the Pearl River Mouth Basin,South China Sea,have identified concentrated gas hydrates with variable thickness.Moreover,free gas and the coexistence of gas hydrate and free gas have been confirmed by logging,coring,and production tests in the foraminifera-rich silty sediments with complex bottom-simulating reflectors(BSRs).The broad-band processing is conducted on conventional three-dimensional(3D)seismic data to improve the image and detection accuracy of gas hydratebearing layers and delineate the saturation and thickness of gas hydrate-and free gas-bearing sediments.Several geophysical attributes extracted along the base of the gas hydrate stability zone are used to demonstrate the variable distribution and the controlling factors for the differential enrichment of gas hydrate.The inverted gas hydrate saturation at the production zone is over 40% with a thickness of 90 m,showing the interbedded distribution with different boundaries between gas hydrate-and free gas-bearing layers.However,the gas hydrate saturation value at the adjacent canyon is 70%,with 30-m-thick patches and linear features.The lithological and fault controls on gas hydrate and free gas distributions are demonstrated by tracing each gas hydrate-bearing layer.Moreover,the BSR depths based on broad-band reprocessed 3D seismic data not only exhibit variations due to small-scale topographic changes caused by seafloor sedimentation and erosion but also show the upward shift of BSR and the blocky distribution of the coexistence of gas hydrate and free gas in the Pearl River Mouth Basin.展开更多
基金supported by the National Science Foundation of China(Nos.81373970,81773902,81973484,and 32171402)the National College Students Innovation and Entrepreneurship Training Program(No.201810315019)+4 种基金the Postgraduate Research and Practice Innovation Program of Jiangsu Province(Nos.SJCX21_0712 and KYCX23_2052)the Scientific Research Project of Jiangsu Provincial Association of Traditional Chinese Medicine(No.XYLD2024013)the Youth Scientific Research Project of Jiangyin Municipal Health Commission(No.Q202402)the Natural Science Foundation Project of Nanjing University of Chinese Medicine(No.XZR2024173)the Jiangyin Science and Technology Innovation Special Fund Project(No.JY0603A011014230032PB),China.
文摘Bone repair remains an important target in tissue engineering,making the development of bioactive scaffolds for effective bone defect repair a critical objective.In this study,β-tricalcium phosphate(β-TCP)scaffolds incorporated with processed pyritum decoction(PPD)were fabricated using three-dimensional(3D)printing-assisted freeze-casting.The produced composite scaffolds were evaluated for their mechanical strength,physicochemical properties,biocompatibility,in vitro proangiogenic activity,and in vivo efficacy in repairing rabbit femoral defects.They not only demonstrated excellent physicochemical properties,enhanced mechanical strength,and good biosafety but also significantly promoted the proliferation,migration,and aggregation of pro-angiogenic human umbilical vein endothelial cells(HUVECs).In vivo studies revealed that all scaffold groups facilitated osteogenesis at the bone defect site,with theβ-TCP scaffolds loaded with PPD markedly enhancing the expression of neurogenic locus Notch homolog protein 1(Notch1),vascular endothelial growth factor(VEGF),bone morphogenetic protein-2(BMP-2),and osteopontin(OPN).Overall,the scaffolds developed in this study exhibited strong angiogenic and osteogenic capabilities both in vitro and in vivo.The incorporation of PPD notably promoted the angiogenic-osteogenic coupling,thereby accelerating bone repair,which suggests that PPD is a promising material for bone repair and that the PPD/β-TCP scaffolds hold great potential as a bone graft alternative.
基金supported by the Start-up Fund from Hainan University(No.KYQD(ZR)-20077)。
文摘Three-dimensional(3D)single molecule localization microscopy(SMLM)plays an important role in biomedical applications,but its data processing is very complicated.Deep learning is a potential tool to solve this problem.As the state of art 3D super-resolution localization algorithm based on deep learning,FD-DeepLoc algorithm reported recently still has a gap with the expected goal of online image processing,even though it has greatly improved the data processing throughput.In this paper,a new algorithm Lite-FD-DeepLoc is developed on the basis of FD-DeepLoc algorithm to meet the online image processing requirements of 3D SMLM.This new algorithm uses the feature compression method to reduce the parameters of the model,and combines it with pipeline programming to accelerate the inference process of the deep learning model.The simulated data processing results show that the image processing speed of Lite-FD-DeepLoc is about twice as fast as that of FD-DeepLoc with a slight decrease in localization accuracy,which can realize real-time processing of 256×256 pixels size images.The results of biological experimental data processing imply that Lite-FD-DeepLoc can successfully analyze the data based on astigmatism and saddle point engineering,and the global resolution of the reconstructed image is equivalent to or even better than FD-DeepLoc algorithm.
基金Supported by the National Natural Science Foundation of China(Nos.42250710152,42192562)the Southern Laboratory of Ocean Science and Engineering(Guangdong Zhuhai)(No.SML 2020 SP 007)。
文摘Convectively unstable processes caused by dense water subsidence are common occurrences in high-latitude oceanic regions,and significantly modulate mass and heat transport and mixing processes in the ocean.An idealized numerical experiment using the large eddy simulation method was conducted to analyze the three-dimensional flow field structure and the mechanism for dense water subsidence.Specifically,a negative salt flux is set at the sea surface,in which salt flux enters the sea surface to simulate the icing and salting-out phenomena that occur at high latitudes.Results show that the mean-state 3D flow field of dense water subsidence exhibits a hollow conical distribution.The horizontal flow field is characterized by a cyclonic vortex that driven primarily by the pressure gradient and influenced by the Coriolis effect.Moreover,the inverse vertical pressure gradient generated by this vortex inhibits the sinking of the plume,leading to its off-axis deflection and the development of an anticyclonic precession.In addition,the impact of rotation on the structure of a sinking plume within a stratified environment is discussed.Both horizontal vortex intensity and cone angle of the hollow cone flow field are increased with increasing rotation rate,resulting in a decrease in the plume’s maximum sinking depth.Variances in rotation direction cause the horizontal vortex and sinking plumes of dense water in the northern and southern hemispheres to rotate in opposite directions.
基金Project(50625101) supported by the National Science Fund for Distinguished Young Scholars of ChinaProject supported by Graduate Independent Innovation Foundation of Shandong University(GIIFSDU),ChinaProject(51071097) supported by the National Natural Science Foundation of China
文摘A series of Al-Ti-B master alloys were prepared by different preparation routes,and the TiB2 particles in the master alloys were extracted and analyzed.It is found that the forming process has significant influence on the three-dimensional morphology of TiB2 particles.Different preparation routes result in different reaction forms,which accounts for the morphology variation of TiB2 particles.When the Al-Ti-B master alloy is prepared using ＂halide salt＂ route,TiB2 particles exhibit hexagonal platelet morphology and are independent with each other.In addition,the reaction temperature almost does not have influence on the morphology of TiB2 particles.However,TiB2 particles exhibit different morphologies at different reaction temperatures when the master alloys are prepared with Al-3B and Ti sponge.When the master alloy is prepared at 850 ℃,a kind of TiB2 particle agglomeration forms with a size larger than 5 μm.The TiB2 particles change to layered stacking morphology even dendritic morphology with the reaction temperature reaching up to 1200 ℃.
基金Project(CSTC 2010BB4301) supported by Natural Science Foundation Project of Chongqing,ChinaProject supported by the Open Fund for Key Laboratory of Manufacture and Test Techniques for Automobile Parts of Ministry of Education Chongqing University of Technology,2003,China
文摘To study the influence of roll casting process parameters on temperature and thermal-stress fields for the AZ31 magnesium alloy sheets,three-dimensional geometric and 3D finite element models for roll casting were established based on the symmetry of roll casting by ANSYS software.Meshing method and smart-sizing algorithm were used to divide finite element mesh in ANSYS software.A series of researches on the temperature and stress distributions during solidification process with different process parameters were done by 3D finite element method.The temperatures of both the liquid-solid two-phase zone and liquid phase zone were elevated with increasing pouring temperature.With the heat transfer coefficient increasing,the two-phase region for liquid-solid becomes smaller.With the pouring temperature increasing and the increase of casting speed,the length of two-phase zone rises.The optimized of process parameters（casting speed 2 m/min,pouring temperature 640 ℃ and heat transfer coefficient 15 kW/（m2·℃） with the water pouring at roller exit was used to produce magnesium alloy AZ31 sheet,and equiaxed grains with the average grain size of 50 μm were achieved after roll casting.The simulation results give better understanding of the temperature variation in phase transformation zone and the formation mechanism of hot cracks in plates during roll casting and help to design the optimized process parameters of roll casting for Mg alloy.
基金This work was financially supported by the National Natural Science Foundation of China (No.50604007)the Natural ScienceFoundation of Liaoning Province, China (No.20062016)
文摘A self-designed setup of modified sloping cooling/shearing process was made to prepare the semisolid Al-3wt%Mg alloy. A three-dimensional simulation model was established for the analysis of preparing the semisolid Al-3wt%Mg alloy. Through simulation and experiment, it is shown that the sloping angle of the plate greatly affects temperature and velocity distributions, and the temperature and velocity of the alloy at the exit of the sloping plate increase with the increase of the sloping angle. The alloy temperature decreases linearly from the pouring mouth to the exit. The alloy temperature at the exit increases obviously with the increase of pouring temperature. To prepare the semisolid Al-3wt%Mg alloy with good quality, the sloping angle θ=45° is reasonable, and the pouring temperature is suggested to be designed above 650-660℃ but under 700℃.
基金This work was supported by the Brain Korea 2lProject and the Grallt of Post-Doc Program, KyungpookNational University (1999).
文摘Some techniques such as die surface description, contact judgement algorithm and remeshing are proposed to improve the robustness of the numerical solution. Based on these techniques, a three-dimensional rigid-plastic FEM code has been developed. Isothermal forging process of a cylindrical housing has been simulated. The simulation results show that the given techniques and the FEM code are reasonable and feasible for three-dimensional bulk forming processes.
基金supported by the National Natural Science Foundation of China(Grant Nos.61274106,11175138,and 61601352)
文摘The fabrication process dependent effects on single event effects （SEEs） are investigated in a commercial silicon- germanium heterojunction bipolar transistor （SiGe HBT） using three-dimensional （3D） TCAD simulations. The influences of device structure and doping concentration on SEEs are discussed via analysis of current transient and charge collection induced by ions strike. The results show that the SEEs representation of current transient is different from representation of the charge collection for the same process parameters. To be specific, the area of C/S junction is the key parameter that affects charge collection of SEE. Both current transient and charge collection are dependent on the doping of collector and substrate. The base doping slightly influences transient currents of base, emitter, and collector terminals. However, the SEEs of SiGe HBT are hardly affected by the doping of epitaxial base and the content of Ge.
基金Supported by the National Defense Basic Scientific Research Project(A1020131011)
文摘A method of constructing three-dimensional process model for the punching cartridge cases is presented based on DEFORM simulation analysis. Using DEFORM software,the finite element simulation models for the punching and forming process of cartridge cases are established,and the corresponding simulation result model of each intermediate procedure is obtained by continuously performing the forming process simulation. The simulation model cannot annotate size and process information due to poor interface between DEFORM software and CAD software. Thus,a 3D annotation module is developed with secondary development technology of UG NX software. Consequently,the final process model with dimension and process information is obtained. Then,with the current 3D process management system,the 3D punching and forming process design of cartridge cases can be completed further. An example is also provided to illustrate that the relative error between the simulation process model and the physical model is less than 2%,which proves the validity and reliability of the proposed method in this study.
基金supported by the National 973 Fundamental Research Program of China (No.2005CB724102,2006CB705404)
文摘The traditional fuzzy logic system （FLS） can only model and control the process in two-dimensional nature. Many of real-world systems are of multidimensional features, such as, thermal and fluid processes with spatiotemporal dynamics, biological systems, or decision-making processes that contain stochastic and imprecise uncertainties. These types of systems are difficult for the traditional FLS to model and control because they require a third dimension for spatial or probabilistic information. The type-2 fuzzy set provides the possibility to develop a three-dimensional fuzzy logic system for modeling and controlling these processes in three-dimensional nature.
基金financial support from the National Natural Science Foundation of China (Nos.51904335,41630642)。
文摘True-triaxial compression tests were carried out on cubic granite samples with a circular through hole using a true-triaxial testing system to investigate the influence of saturated water content(SWC) on the failure process and characteristics of a circular tunnel of surrounding rocks. The spalling failure under SWC can be divided into four periods: calm period, buckling deformation period, period of rock fragment gradual buckling and exfoliation, and period of formation of symmetrical V-shaped notches. When the horizontal axial and vertical stresses were constant, the spalling failure severity was reduced with the increase in lateral stress. Under natural water content, a strong rockburst with dynamic failure characteristics occurred on the circular hole sidewall. Under SWC, the failure severity was reduced and the circular hole sidewall experienced spalling failure, exhibiting progressive static failure characteristics.Therefore, water can reduce the failure severity of surrounding rocks in deep underground engineering, which has a certain guiding significance for the prevention and control of rockbursts.
基金financially supported by the National Natural Science Foundation of China (No.51875124)the National Key Research and Development Project,China (No.2019YFB2006503)。
文摘To shorten the preparation process of semi-solid billets,semi-solid billets of 2A14 aluminum alloy were prepared by wrought aluminum directly semi-solid isothermal treatment(WADSSIT)process.Three-dimension(3D)combined microstructure evolution,namely transverse direction(TD)surface,rolling direction(RD)surface,and normal direction(ND)surface,was studied.Effects of temperature and holding time on average grain size and average shape factor were investigated.The results showed that the optimum conditions for preparation of 2A14 semi-solid billets by this process were 615℃ and 20 min(average grain size of 124μm and shape factor of 0.81).Electron backscatter diffraction(EBSD)observations indicated that the microstructure was completely recrystallized when it was heated to 600℃.Grain size was increased with the increase of temperature and grew up slowly with the holding time prolonging.Roundness was increased with increase of holding time but was not sensitive to temperature.
文摘The extremely high peak intensity associated with ultrashort pulse width of femtosecond(fs)lasers enabled inducing nonlinear multiphoton absorption in materials that are transparent to the laser wavelength.More importantly,focusing the fs laser beam inside the transparent materials confined the nonlinear interaction to within the focal volume only,realizing three-dimensional(3D)micro/nanofabrication.This 3D capability offers three different processing schemes for use in fabrication:undeformative,subtractive,and additive.Furthermore,a hybrid approach of different schemes can create much more complex 3D structures and thereby promises to enhance the functionality of the structures created.Thus,hybrid fs laser 3D microprocessing opens a new door for material processing.This paper comprehensively reviews different types of hybrid fs laser 3D micro/nanoprocessing for diverse applications including fabrication of functional micro/nanodevices.
文摘To address the problem of multi-missile cooperative interception against maneuvering targets at a prespecified impact time and desired Line-of-Sight(LOS)angles in ThreeDimensional(3D)space,this paper proposes a 3D leader-following cooperative interception guidance law.First,in the LOS direction of the leader,an impact time-controlled guidance law is derived based on the fixed-time stability theory,which enables the leader to complete the interception task at a prespecified impact time.Next,in the LOS direction of the followers,by introducing a time consensus tracking error function,a fixed-time consensus tracking guidance law is investigated to guarantee the consensus tracking convergence of the time-to-go.Then,in the direction normal to the LOS,by combining the designed global integral sliding mode surface and the second-order Sliding Mode Control(SMC)theory,an innovative 3D LOS-angle-constrained interception guidance law is developed,which eliminates the reaching phase in the traditional sliding mode guidance laws and effectively saves energy consumption.Moreover,it effectively suppresses the chattering phenomenon while avoiding the singularity issue,and compensates for unknown interference caused by target maneuvering online,making it convenient for practical engineering applications.Finally,theoretical proof analysis and multiple sets of numerical simulation results verify the effectiveness,superiority,and robustness of the investigated guidance law.
文摘Air separators provide safe, clean, and appropriate air flow to engines and are widely used in vehicles with large engines such as ships and submarines. In this operational study, the separation process in a Ranque-Hilsch vortex tube cleaning （cooling） system is investigated to analyze the impact of the operating gas type on the vortex tube performance; the operating gases used are air, nitrogen, oxygen, carbon dioxide and nitrogen dioxide. The computational fluid dynamic model used is equipped with a three-dimensional structure, and the steady-state condition is applied during computations. The standard k-c turbulence model is employed to resolve nonlinear flow equations, and various key parameters, such as hot and cold exhaust thermal drops, and power separation rates, are described numerically. The results show that nitrogen dioxide creates the greatest separation power out of all gases tested, and the numerical results are validated by good agreement with available experimental data. In addition, a comparison is made between the use of two different boundary conditions, the pressure-far-field and the pressure-outlet, when analyzing complex turbulent flows in the air separators. Results present a comprehensive and practical solution for use in future numerical studies.
基金partially supported by the National Key Research&Development Plan of China(Grant No.2017YFC0804203)International Cooperation Project of Chinese Academy of Sciences(Grant No.115242KYSB20160024)the Open Fund of State Key Laboratory of Geomechanics and Geotechnical Engineering,Institute of Rock and Soil Mechanics,Chinese Academy of Sciences(Grant No.Z016003)
文摘In this context, recent developments in the coupled three-dimensional(3 D) hydro-mechanical(HM)simulation tool TOUGH-RBSN are presented. This tool is used to model hydraulic fracture in geological media, as observed in laboratory-scale tests. The TOUGH-RBSN simulator is based on the effective linking of two numerical methods: TOUGH2, a finite volume method for simulating mass transport within a permeable medium; and a lattice model based on the rigid-body-spring network(RBSN) concept. The method relies on a Voronoi-based discretization technique that can represent fracture development within a permeable rock matrix. The simulator provides two-way coupling of HM processes, including fluid pressure-induced fracture and fracture-assisted flow. We first present the basic capabilities of the modeling approach using two example applications, i.e. permeability evolution under compression deformation, and analyses of a static fracturing simulation. Thereafter, the model is used to simulate laboratory tests of hydraulic fracturing in granite. In most respects, the simulation results meet expectations with respect to permeability evolution and fracturing patterns. It can be seen that the evolution of injection pressure associated with the simulated fracture developments is strongly affected by fluid viscosity.
基金This work was supported by the National Natural Science Foundation of China （No. 50275094）.
文摘A meshless approach, called the rigid-plastic reproducing kernel particle method （RKPM）, is presented for three-dimensional （3D） bulk metal forming simulation. The approach is a combination of RKPM with the flow theory of 3D rigid-plastic mechanics. For the treatments of essential boundary conditions and incompressibility constraint, the boundary singular kernel method and the modified penalty method are utilized, respectively. The arc-tangential friction model is employed to treat the contact conditions. The compression of rectangular blocks, a typical 3D upsetting operation, is analyzed for different friction conditions and the numerical results are compared with those obtained using commercial rigid-plastic FEM （finite element method） software Deform^3D. As results show, when handling 3D plastic deformations, the proposed approach eliminates the need of expensive meshing and remeshing procedures which are unavoidable in conventional FEM and can provide results that are in good agreement with finite element predictions.
文摘Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma pathobiology.Conventional cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under discussion.In vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture.However,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)characteristics.Therefore,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between them.Given the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development.In this review,we first provide a general overview of 3D cell cultures compared to 2D cell cultures.We then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO methodologies.Finally,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma.
基金supported by Guangxi Science and Technology Major Program(No.AA23073008)Hubei Key Laboratory of Water System Science for Sponge City Construction(Wuhan University)(No.2023–05)Nanning Innovation and Entrepreneur Leading Talent Project(No.2021001).
文摘The three-dimensional particle electrode system exhibits significant potential for application in the treatment of wastewater.Nonetheless,the advancement of effective granular electrodes characterized by elevated catalytic activity and minimal energy consumption continues to pose a significant challenge.In this research,Fluorine-doped copper-carbon(F/Cu-GAC)particle electrodes were effectively synthesized through an impregnationcalcination technique,utilizing granular activated carbon as the carrier and fluorinedoped modified copper oxides as the catalytic agents.The particle electrodes were subsequently utilized to promote the degradation of 2,4,6-trichlorophenol(2,4,6-TCP)in a threedimensional electrocatalytic reactor(3DER).The F/Cu-GAC particle electrodes were polarized under the action of electric field,which promoted the heterogeneous Fenton-like reaction in which H2O2 generated by two-electron oxygen reduction reaction(2e-ORR)of O_(2) was catalytically decomposed to·OH.The 3DER equipped with F/Cu-GAC particle electrodes showed 100%removal of 2,4,6-TCP and 79.24%removal of TOC with a specific energy consumption(EC)of approximately 0.019 kWh/g·COD after 2 h of operation.The F/Cu-GAC particle electrodes exhibited an overpotential of 0.38 V and an electrochemically active surface area(ECSA)of 715 cm^(2),as determined through linear sweep voltammetry(LSV)and cyclic voltammetry(CV)assessments.These findings suggest a high level of electrocatalytic performance.Furthermore,the catalytic mechanism of the 3DER equipped with F/Cu-GAC particle electrodes was elucidated through the application of X-ray photoelectron spectroscopy(XPS),electron spin resonance(ESR),and active species capture experiments.This investigation offers a novel approach for the effective degradation of 2,4,6-TCP.
基金supported by the State Key Laboratory of Natural Gas Hydrate(No.2022-KFJJ-SHW)the National Natural Science Foundation of China(No.42376058)+2 种基金the International Science&Technology Cooperation Program of China(No.2023YFE0119900)the Hainan Province Key Research and Development Project(No.ZDYF2024GXJS002)the Research Start-Up Funds of Zhufeng Scholars Program.
文摘Gas hydrate drilling expeditions in the Pearl River Mouth Basin,South China Sea,have identified concentrated gas hydrates with variable thickness.Moreover,free gas and the coexistence of gas hydrate and free gas have been confirmed by logging,coring,and production tests in the foraminifera-rich silty sediments with complex bottom-simulating reflectors(BSRs).The broad-band processing is conducted on conventional three-dimensional(3D)seismic data to improve the image and detection accuracy of gas hydratebearing layers and delineate the saturation and thickness of gas hydrate-and free gas-bearing sediments.Several geophysical attributes extracted along the base of the gas hydrate stability zone are used to demonstrate the variable distribution and the controlling factors for the differential enrichment of gas hydrate.The inverted gas hydrate saturation at the production zone is over 40% with a thickness of 90 m,showing the interbedded distribution with different boundaries between gas hydrate-and free gas-bearing layers.However,the gas hydrate saturation value at the adjacent canyon is 70%,with 30-m-thick patches and linear features.The lithological and fault controls on gas hydrate and free gas distributions are demonstrated by tracing each gas hydrate-bearing layer.Moreover,the BSR depths based on broad-band reprocessed 3D seismic data not only exhibit variations due to small-scale topographic changes caused by seafloor sedimentation and erosion but also show the upward shift of BSR and the blocky distribution of the coexistence of gas hydrate and free gas in the Pearl River Mouth Basin.
