为鉴定我国黑龙江省分离的狍子源鞭虫的种类及确定其在鞭虫中的分类地位,本研究首先对鞭虫进行形态学特征观察,并利用PCR方法扩增该鞭虫r DNA ITS序列,经测序分析结果表明,r DNA ITS序列全长为1 371 bp,其中ITS1、5.8S及ITS2大小分别为8...为鉴定我国黑龙江省分离的狍子源鞭虫的种类及确定其在鞭虫中的分类地位,本研究首先对鞭虫进行形态学特征观察,并利用PCR方法扩增该鞭虫r DNA ITS序列,经测序分析结果表明,r DNA ITS序列全长为1 371 bp,其中ITS1、5.8S及ITS2大小分别为800 bp、163 bp及408 bp,与之前报道的狍子源鞭虫同源性达99.8%。将其与NCBI数据库中相应鞭虫ITS序列进行比对分析,并将r DNA ITS序列作为基因标记构建鞭虫属系统发生树,结果显示本研究所分离的狍子源鞭虫与其他不同宿主的无色鞭虫均处于同一个分支,因此可鉴定本研究分离的我国狍子源鞭虫为无色鞭虫。本研究为国内首次对狍子源鞭虫进行分子鉴定及系统进化分析,为草食动物的鞭虫病分子鉴别诊断和鞭虫系统进化分析研究提供依据。展开更多
Soil harbours enormous biodiversity,essential for maintaining environmental and human health.However,soil can also be a reservoir of various parasitic pathogens,such as soil-transmitted helminths(STH).We evaluated the...Soil harbours enormous biodiversity,essential for maintaining environmental and human health.However,soil can also be a reservoir of various parasitic pathogens,such as soil-transmitted helminths(STH).We evaluated the presence of STH(e.g.,hookworms,roundworms and whipworms)in soil samples collected at twenty points within the perimeter of Campus do Vale(a university campus belonging to the Federal University of Rio Grande do Sul-UFRGS),during 2022 winter season.Considering the One Health perspective,human,animal and environmentrelated data from each sampling point were collected.All soil samples showed nematode larvae,representing natural components of soil biodiversity.Considering STH eggs,35%(n=7)of soil samples showed hookworm eggs(e.g.,from Necator americanus or Ancylostoma duodenale),10%(n=2)showed roundworm(Ascaris lumbricoides)eggs,and 5%(n=1)showed whipworm(Trichuris trichiura-like)eggs.Of note,10%of the sampling points showed the presence of rhabditiform hookworm larvae,5%showed Strongyloides stercoralis rhabditiform larvae and 5%had the presence of filariform hookworm larvae,indicating a risk of human percutaneous infection.The significant people circulation in Campus do Vale,in association with other environment-related factors,help to explain the prevalence of STH observed in this study.展开更多
文摘为鉴定我国黑龙江省分离的狍子源鞭虫的种类及确定其在鞭虫中的分类地位,本研究首先对鞭虫进行形态学特征观察,并利用PCR方法扩增该鞭虫r DNA ITS序列,经测序分析结果表明,r DNA ITS序列全长为1 371 bp,其中ITS1、5.8S及ITS2大小分别为800 bp、163 bp及408 bp,与之前报道的狍子源鞭虫同源性达99.8%。将其与NCBI数据库中相应鞭虫ITS序列进行比对分析,并将r DNA ITS序列作为基因标记构建鞭虫属系统发生树,结果显示本研究所分离的狍子源鞭虫与其他不同宿主的无色鞭虫均处于同一个分支,因此可鉴定本研究分离的我国狍子源鞭虫为无色鞭虫。本研究为国内首次对狍子源鞭虫进行分子鉴定及系统进化分析,为草食动物的鞭虫病分子鉴别诊断和鞭虫系统进化分析研究提供依据。
基金Marina Ziliotto received a fellowship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-CAPES(Brazil).Joel Henrique Ellwanger receives a postdoctoral fellowship from CAPES(Programa Nacional de Pós-Doutorado-PNPD/CAPES,Brazil).JoséArtur Bogo Chies receives a research fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq(Bolsa de Produtividade em Pesquisa-Nível 1A,CNPq,Brazil)and has research project funded by CAPES(CAPES AUXPE 686/2020,Brazil).
文摘Soil harbours enormous biodiversity,essential for maintaining environmental and human health.However,soil can also be a reservoir of various parasitic pathogens,such as soil-transmitted helminths(STH).We evaluated the presence of STH(e.g.,hookworms,roundworms and whipworms)in soil samples collected at twenty points within the perimeter of Campus do Vale(a university campus belonging to the Federal University of Rio Grande do Sul-UFRGS),during 2022 winter season.Considering the One Health perspective,human,animal and environmentrelated data from each sampling point were collected.All soil samples showed nematode larvae,representing natural components of soil biodiversity.Considering STH eggs,35%(n=7)of soil samples showed hookworm eggs(e.g.,from Necator americanus or Ancylostoma duodenale),10%(n=2)showed roundworm(Ascaris lumbricoides)eggs,and 5%(n=1)showed whipworm(Trichuris trichiura-like)eggs.Of note,10%of the sampling points showed the presence of rhabditiform hookworm larvae,5%showed Strongyloides stercoralis rhabditiform larvae and 5%had the presence of filariform hookworm larvae,indicating a risk of human percutaneous infection.The significant people circulation in Campus do Vale,in association with other environment-related factors,help to explain the prevalence of STH observed in this study.
