Non-steroidal anti-inflammatory drugs（NSAIDs） were able to produce tissue damage and oxidative stress in animal models of small intestinal damage. In this study, the putative protective effect of wheat peptides was ...Non-steroidal anti-inflammatory drugs（NSAIDs） were able to produce tissue damage and oxidative stress in animal models of small intestinal damage. In this study, the putative protective effect of wheat peptides was evaluated in a NSAID-induced small intestinal damage model in rats, different doses of wheat peptides or distilled water were administered daily by intragastric administration for 30 d until small intestinal damage was caused. Before sacrificing, NSAIDs（aspirin and indomethacin） or physiological saline were infused into the digestive tract twice. Wheat peptides administration reduced edema and small intestinal damage, and significantly decreased the level of tumor necrosis factor（TNF）-α in mucous membrane of small intestine. Oxidative stress was significantly increased after NSAID infusion and was reduced by wheat peptides. Wheat peptides increased glutathione peroxidase（GSH-Px） activity in mucous membrane of small intestine. μ-Opioid receptor mRNA expression decreased more significantly in wheat peptides treated rats than in the model control group. Overall, the results suggest that non-steroidal anti-inflammatory drugs induced small intestinal damage in rats and wheat peptides administration may be an effective tool for protecting small intestinal tissue against NSAID-induced small intestinal damage and oxidative stress.展开更多
Non-steroidal anti-inflammatory drugs(NSAIDs) induce tissue damage and oxidative stress in animal models of stomach damage. In the present study, the protective effects of wheat peptides were evaluated in a NSAID-indu...Non-steroidal anti-inflammatory drugs(NSAIDs) induce tissue damage and oxidative stress in animal models of stomach damage. In the present study, the protective effects of wheat peptides were evaluated in a NSAID-induced stomach damage model in rats. Different doses of wheat peptides or distilled water were administered daily by gavage for 30 days before the rat stomach damage model was established by administration of NSAIDs(aspirin and indomethacin) into the digestive tract twice. The treatment of wheat peptides decreased the NSAID-induced gastric epithelial cell degeneration and oxidative stress and NO levels in the rats. Wheat peptides significantly increased the superoxide dismutase(SOD) and glutathione peroxidase(GPx) activities and decreased i NOS activity in stomach. The m RNA expression level of μ-opioid receptor was significantly decreased in wheat peptides-treated rats than that in in the control rats. The results suggest that NSAID drugs induced stomach damage in rats, wchih can be prevented by wheat peptides. The mechanisms for the protective effects were most likely through reducing NSAID-induced oxidative stress.展开更多
Constipation has become more common in recent decades,affecting people’s quality of life.Natural bioactive substances could be effective in alleviating constipation.The goal of this study was to assess and compare th...Constipation has become more common in recent decades,affecting people’s quality of life.Natural bioactive substances could be effective in alleviating constipation.The goal of this study was to assess and compare the efficacy of low and high hydrolysis degree wheat peptides(WP-L and WP-H)at a dose of 1 mg/g.bw on relieving loperamide-induced constipation in mice and the underlying mechanisms.Here,we proved that both WP-L and WP-H treatment showed constipation relieving effect by improving defecation function and small intestinal propulsion rate while the different hydrolysis degree did not affect the efficacy of WP.Moreover,WP-L and WP-H could balance the secretion of excitatory and inhibitory factors,and enhance the activity of antioxidant enzyme(SOD and GSH-Px).Importantly,both of them could accelerate intestinal motility,improve water-salt metabolism and intestinal barrier,and ameliorate oxidative stress.In summary,our findings indicated that 1 mg/g.bw WP-L and WP-H effectively relieved loperamide-induced constipation by affecting multiple targets in mice.展开更多
基金sponsored by grants from Postgraduates Scientific Research and Innovation Projects in JiangsuProvince,China(CXZZ12_0124)
文摘Non-steroidal anti-inflammatory drugs（NSAIDs） were able to produce tissue damage and oxidative stress in animal models of small intestinal damage. In this study, the putative protective effect of wheat peptides was evaluated in a NSAID-induced small intestinal damage model in rats, different doses of wheat peptides or distilled water were administered daily by intragastric administration for 30 d until small intestinal damage was caused. Before sacrificing, NSAIDs（aspirin and indomethacin） or physiological saline were infused into the digestive tract twice. Wheat peptides administration reduced edema and small intestinal damage, and significantly decreased the level of tumor necrosis factor（TNF）-α in mucous membrane of small intestine. Oxidative stress was significantly increased after NSAID infusion and was reduced by wheat peptides. Wheat peptides increased glutathione peroxidase（GSH-Px） activity in mucous membrane of small intestine. μ-Opioid receptor mRNA expression decreased more significantly in wheat peptides treated rats than in the model control group. Overall, the results suggest that non-steroidal anti-inflammatory drugs induced small intestinal damage in rats and wheat peptides administration may be an effective tool for protecting small intestinal tissue against NSAID-induced small intestinal damage and oxidative stress.
基金supported by the grants from Postgraduates scientific research and innovation projects in Jiangsu Province(No:CXZZ12_0124)the Fundamental Research Funds for the Central Universities
文摘Non-steroidal anti-inflammatory drugs(NSAIDs) induce tissue damage and oxidative stress in animal models of stomach damage. In the present study, the protective effects of wheat peptides were evaluated in a NSAID-induced stomach damage model in rats. Different doses of wheat peptides or distilled water were administered daily by gavage for 30 days before the rat stomach damage model was established by administration of NSAIDs(aspirin and indomethacin) into the digestive tract twice. The treatment of wheat peptides decreased the NSAID-induced gastric epithelial cell degeneration and oxidative stress and NO levels in the rats. Wheat peptides significantly increased the superoxide dismutase(SOD) and glutathione peroxidase(GPx) activities and decreased i NOS activity in stomach. The m RNA expression level of μ-opioid receptor was significantly decreased in wheat peptides-treated rats than that in in the control rats. The results suggest that NSAID drugs induced stomach damage in rats, wchih can be prevented by wheat peptides. The mechanisms for the protective effects were most likely through reducing NSAID-induced oxidative stress.
基金This work was supported by the Ningbo Science and Technology Innovation 2025 Major Special Project[grant numbers 2019B10060].
文摘Constipation has become more common in recent decades,affecting people’s quality of life.Natural bioactive substances could be effective in alleviating constipation.The goal of this study was to assess and compare the efficacy of low and high hydrolysis degree wheat peptides(WP-L and WP-H)at a dose of 1 mg/g.bw on relieving loperamide-induced constipation in mice and the underlying mechanisms.Here,we proved that both WP-L and WP-H treatment showed constipation relieving effect by improving defecation function and small intestinal propulsion rate while the different hydrolysis degree did not affect the efficacy of WP.Moreover,WP-L and WP-H could balance the secretion of excitatory and inhibitory factors,and enhance the activity of antioxidant enzyme(SOD and GSH-Px).Importantly,both of them could accelerate intestinal motility,improve water-salt metabolism and intestinal barrier,and ameliorate oxidative stress.In summary,our findings indicated that 1 mg/g.bw WP-L and WP-H effectively relieved loperamide-induced constipation by affecting multiple targets in mice.
