The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Ni...The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith)) leaf disks. We compared the effect of temperatures ranging from 15°C, 18°C, 20°C, 22°C to 25°C on the stable expression of β-glucuronidase (GUS) activity of 14 days old hygromycin-selected leaf disks, and on the increase in the fresh weight yield of 28 days old kanamycin-selected calli. The highest average of GUS activity was obtained at 20°C among the five temperatures tested although the difference between the 18°C and 20°C treatment was not statistically significant. The GUS activity at 15°C was statistically lower than those at 18°C and 20°C. The GUS activity in 22°C treatment was an intermediate between the highest (18/20°C) and second highest averages (15°C), and was not statistically significantly different. The lowest average of GUS activity was observed at 25°C. The highest increase in the plate average of fresh weight yield was obtained at 20°C among the five temperature tested. The 20°C treatment was statistically significantly better than the 15°C and 18°C treatments. The 20°C co-cultivation treatment resulted in the higher FW yield than 22°C and 25°C even though the differences were not statistically significant. In conclusion, low co-cultivation temperature at 20°C resulted in the reproducible maximum increase in both the fresh weight yield and stable expression of GUS activity after transformation of tobacco leaf disks.展开更多
Büeckner Rice weight function method was used to analyse mixed mode fracture of center cracked circular disk subjected to uniaxial compression. Based on Wu Carlsson procedure semi analytical modes Ⅰ and Ⅱ weigh...Büeckner Rice weight function method was used to analyse mixed mode fracture of center cracked circular disk subjected to uniaxial compression. Based on Wu Carlsson procedure semi analytical modes Ⅰ and Ⅱ weight functions were derived from corresponding reference displacement fields and stress intensity factors calculated by finite element method. Normalized mode Ⅰ and mode Ⅱ stress intensity factors, f Ⅰ, f Ⅱ , were derived from the obtained semi analytical weight functions. The results were then fitted into polynomials, the precision is within 0.5%. It is interesting to note that when the inclined angle θ of a crack is less than 15°, the f Ⅰvalues are positive. when θ =15°, the f Ⅰ values are positive for the crack length a varying from 0.1 to 0.7, but when a =0.8, the f Ⅰ takes the negative value -0.51. When θ >15°, all the f Ⅰ values become negative, which denotes that the compression shear mode is achieved at crack tips. These results are very useful in the investigation of mixed mode fracture of brittle materials.展开更多
A new specimen geometry-the double edge-cracked Brazilian disk and a relevant fracture analysis byweight function method are proposed for the investigation of rock fracture caused by compression-shear loading. Notonly...A new specimen geometry-the double edge-cracked Brazilian disk and a relevant fracture analysis byweight function method are proposed for the investigation of rock fracture caused by compression-shear loading. Notonly can the mixed mode fracture with any ratio of KⅠ/KⅡ be achieved, but also the pure mode Ⅱ crack extensioncan be obtained. The combined mode fracture analysis for this geometry shows that diametral compression in the far-field can induce a compression-shear stress state in the singular stress field ahead of crack tips. Experimental investi-gations conducted on marble specimens show that the pure mode Ⅱ crack extension can be obtained when the dimen-sionless crack length a＞0.7 and the inclined crack angle 5°≤ψ≤40°. Normalized mode Ⅰ and mode Ⅱ stress inten-sity factors decrease from -0.45 and 2.47 at ψ=5° to -1.65 and 1.52 at ψ=40°, respectively. The strains at threepoints of specimen are also measured in order to investigate the influence of stress singularity on initial crack exten-sion. The results show that the principal orientations of strain at three points are very stable in the loading process.The derived formulae are quite explicit, and the specimen geometry is easy to fabricate and convenient to achieve thepure mode Ⅱ crack extension. Therefore, it can hopefully be used to obtain mode Ⅱ fracture toughness of rock.展开更多
A detailed analysis of mode II stress intensity factors(SIFs) for the double edge cracked Brazilian disk subjected to different diametral compression is presented using a weight function method. The mode II SIFs at cr...A detailed analysis of mode II stress intensity factors(SIFs) for the double edge cracked Brazilian disk subjected to different diametral compression is presented using a weight function method. The mode II SIFs at crack tips can be obtained by simply calculating an integral of the product of mode II weight function and the shear stress on the prospective crack faces of uncracked disk loaded by a diametral compression. A semi-analytical formula for the calculation of normalized mode II SIF, f _Ⅱ, is derived for different crack lengths (from 0.1 to 0.7) and inclination angles (from 10° to 75°) with respect to loading direction. Comparison between the obtained results and finite element method solutions shows that the weight function method is of high precision. Combined with the authors previous work on mode I fracture analysis, the new specimen geometry can be used to study fracture through any combination of mode I and mode II loading by a simple alignment of the crack relative to the diameter of compression loading, and to obtain pure mode II crack extension. Another advantage of this specimen geometry is that it is available directly from rock core and is also easy to fabricate.展开更多
A dynamic weight function method is presented for dynamic stress intensity factors of circular disk with a radial edge crack under external impulsive pressure. The dynamic stresses in a circular disk are solved under ...A dynamic weight function method is presented for dynamic stress intensity factors of circular disk with a radial edge crack under external impulsive pressure. The dynamic stresses in a circular disk are solved under abrupt step external pressure using the eigenfunction method. The solution consists of a quasi-static solution satisfying inhomogeneous boundary conditions and a dynamic solution satisfying homogeneous boundary conditions. By making use of Fourier- Bessel series expansion, the history and distribution of dynamic stresses in the circular disk are derived. Furthermore, the equation for stress intensity factors under uniform pressure is used as the reference case, the weight function equation for the circular disk containing an edge crack is worked out, and the dynamic stress intensity factor equation for the circular disk containing a radial edge crack can be given. The results indicate that the stress intensity factors under sudden step external pressure vary periodically with time, and the ratio of the maximum value of dynamic stress intensity factors to the corresponding static value is about 2.0.展开更多
Let D be the open unit disk in the complex plane C. For a〉 -1, let dAa（z）=（1 ＋a） （1 -｜z}^2） ^a da（z）be the weighted Lebesgue measure on ]D. For a positive function ω ∈ L^1（D,dAa）, the generalized weight...Let D be the open unit disk in the complex plane C. For a〉 -1, let dAa（z）=（1 ＋a） （1 -｜z}^2） ^a da（z）be the weighted Lebesgue measure on ]D. For a positive function ω ∈ L^1（D,dAa）, the generalized weighted Bergman-Orlicz spaceA^ψω（D,dAa）is｜｜f｜｜ω^ψ=∫Dψ｜F（z）｜ω（z）dA^（z） 〈 ∞,where q; is a strictly convex Orlicz function that satisfies other technical hypotheses. Let G be a measurable subset of D, we say G satisfies the reverse Carleson condition for A^ψω （D, dAa） if there exists a positive constant C such that ∫Gψ（f（z））ω（z）dAa（z）≥C∫Dψ（｜f（z）dAa（z）.for all f ∈ .A^ψω （D,dAa）. Let μ be a positive Borel measure, we say μ satisfies the direct Carleson condition if there exists a positive constant M such that for all f∈Aψ^ω （D,dAa）,∫Dψ（｜f（z）｜）dμ（z）≤M∫Dψ（｜f（z）｜）ω（z）dAa（a）.In this paper, we study the direct and reverse Carleson condition on the generalized weighted Bergman-Orlicz space Aω^ψ（D,dAa）.We present conditions on the set G such that＇the reverse Carleson condition＇holds. ＂Moreover, we give a sufficient condition for the finite positive Borel measure μ to satisfy the direct carleson condition on the generalized weighted Bergman-Orlicz spaces.展开更多
文摘The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith)) leaf disks. We compared the effect of temperatures ranging from 15°C, 18°C, 20°C, 22°C to 25°C on the stable expression of β-glucuronidase (GUS) activity of 14 days old hygromycin-selected leaf disks, and on the increase in the fresh weight yield of 28 days old kanamycin-selected calli. The highest average of GUS activity was obtained at 20°C among the five temperatures tested although the difference between the 18°C and 20°C treatment was not statistically significant. The GUS activity at 15°C was statistically lower than those at 18°C and 20°C. The GUS activity in 22°C treatment was an intermediate between the highest (18/20°C) and second highest averages (15°C), and was not statistically significantly different. The lowest average of GUS activity was observed at 25°C. The highest increase in the plate average of fresh weight yield was obtained at 20°C among the five temperature tested. The 20°C treatment was statistically significantly better than the 15°C and 18°C treatments. The 20°C co-cultivation treatment resulted in the higher FW yield than 22°C and 25°C even though the differences were not statistically significant. In conclusion, low co-cultivation temperature at 20°C resulted in the reproducible maximum increase in both the fresh weight yield and stable expression of GUS activity after transformation of tobacco leaf disks.
文摘Büeckner Rice weight function method was used to analyse mixed mode fracture of center cracked circular disk subjected to uniaxial compression. Based on Wu Carlsson procedure semi analytical modes Ⅰ and Ⅱ weight functions were derived from corresponding reference displacement fields and stress intensity factors calculated by finite element method. Normalized mode Ⅰ and mode Ⅱ stress intensity factors, f Ⅰ, f Ⅱ , were derived from the obtained semi analytical weight functions. The results were then fitted into polynomials, the precision is within 0.5%. It is interesting to note that when the inclined angle θ of a crack is less than 15°, the f Ⅰvalues are positive. when θ =15°, the f Ⅰ values are positive for the crack length a varying from 0.1 to 0.7, but when a =0.8, the f Ⅰ takes the negative value -0.51. When θ >15°, all the f Ⅰ values become negative, which denotes that the compression shear mode is achieved at crack tips. These results are very useful in the investigation of mixed mode fracture of brittle materials.
基金Project (50274074) supported by the National Natural Science Foundation of China
文摘A new specimen geometry-the double edge-cracked Brazilian disk and a relevant fracture analysis byweight function method are proposed for the investigation of rock fracture caused by compression-shear loading. Notonly can the mixed mode fracture with any ratio of KⅠ/KⅡ be achieved, but also the pure mode Ⅱ crack extensioncan be obtained. The combined mode fracture analysis for this geometry shows that diametral compression in the far-field can induce a compression-shear stress state in the singular stress field ahead of crack tips. Experimental investi-gations conducted on marble specimens show that the pure mode Ⅱ crack extension can be obtained when the dimen-sionless crack length a＞0.7 and the inclined crack angle 5°≤ψ≤40°. Normalized mode Ⅰ and mode Ⅱ stress inten-sity factors decrease from -0.45 and 2.47 at ψ=5° to -1.65 and 1.52 at ψ=40°, respectively. The strains at threepoints of specimen are also measured in order to investigate the influence of stress singularity on initial crack exten-sion. The results show that the principal orientations of strain at three points are very stable in the loading process.The derived formulae are quite explicit, and the specimen geometry is easy to fabricate and convenient to achieve thepure mode Ⅱ crack extension. Therefore, it can hopefully be used to obtain mode Ⅱ fracture toughness of rock.
文摘A detailed analysis of mode II stress intensity factors(SIFs) for the double edge cracked Brazilian disk subjected to different diametral compression is presented using a weight function method. The mode II SIFs at crack tips can be obtained by simply calculating an integral of the product of mode II weight function and the shear stress on the prospective crack faces of uncracked disk loaded by a diametral compression. A semi-analytical formula for the calculation of normalized mode II SIF, f _Ⅱ, is derived for different crack lengths (from 0.1 to 0.7) and inclination angles (from 10° to 75°) with respect to loading direction. Comparison between the obtained results and finite element method solutions shows that the weight function method is of high precision. Combined with the authors previous work on mode I fracture analysis, the new specimen geometry can be used to study fracture through any combination of mode I and mode II loading by a simple alignment of the crack relative to the diameter of compression loading, and to obtain pure mode II crack extension. Another advantage of this specimen geometry is that it is available directly from rock core and is also easy to fabricate.
文摘A dynamic weight function method is presented for dynamic stress intensity factors of circular disk with a radial edge crack under external impulsive pressure. The dynamic stresses in a circular disk are solved under abrupt step external pressure using the eigenfunction method. The solution consists of a quasi-static solution satisfying inhomogeneous boundary conditions and a dynamic solution satisfying homogeneous boundary conditions. By making use of Fourier- Bessel series expansion, the history and distribution of dynamic stresses in the circular disk are derived. Furthermore, the equation for stress intensity factors under uniform pressure is used as the reference case, the weight function equation for the circular disk containing an edge crack is worked out, and the dynamic stress intensity factor equation for the circular disk containing a radial edge crack can be given. The results indicate that the stress intensity factors under sudden step external pressure vary periodically with time, and the ratio of the maximum value of dynamic stress intensity factors to the corresponding static value is about 2.0.
文摘Let D be the open unit disk in the complex plane C. For a〉 -1, let dAa（z）=（1 ＋a） （1 -｜z}^2） ^a da（z）be the weighted Lebesgue measure on ]D. For a positive function ω ∈ L^1（D,dAa）, the generalized weighted Bergman-Orlicz spaceA^ψω（D,dAa）is｜｜f｜｜ω^ψ=∫Dψ｜F（z）｜ω（z）dA^（z） 〈 ∞,where q; is a strictly convex Orlicz function that satisfies other technical hypotheses. Let G be a measurable subset of D, we say G satisfies the reverse Carleson condition for A^ψω （D, dAa） if there exists a positive constant C such that ∫Gψ（f（z））ω（z）dAa（z）≥C∫Dψ（｜f（z）dAa（z）.for all f ∈ .A^ψω （D,dAa）. Let μ be a positive Borel measure, we say μ satisfies the direct Carleson condition if there exists a positive constant M such that for all f∈Aψ^ω （D,dAa）,∫Dψ（｜f（z）｜）dμ（z）≤M∫Dψ（｜f（z）｜）ω（z）dAa（a）.In this paper, we study the direct and reverse Carleson condition on the generalized weighted Bergman-Orlicz space Aω^ψ（D,dAa）.We present conditions on the set G such that＇the reverse Carleson condition＇holds. ＂Moreover, we give a sufficient condition for the finite positive Borel measure μ to satisfy the direct carleson condition on the generalized weighted Bergman-Orlicz spaces.
