Objective:Herbal medicine is an important therapeutic option for benign prostatic hyperplasia(BPH),a common disease in older men that can seriously affect their quality of life.Currently,it is crucial to develop agent...Objective:Herbal medicine is an important therapeutic option for benign prostatic hyperplasia(BPH),a common disease in older men that can seriously affect their quality of life.Currently,it is crucial to develop agents with strong efficacy and few side effects.Herein we investigated the effects of the extract of Rauwolfia vomitoria,a shrub grown in West Africa,on BPH.Methods:Rats with testosterone-induced BPH were treated with R.vomitoria.Prostates were histologically analyzed by Hematoxylin and eosin staining.Proliferation index and the expression levels of androgen receptor and its associated proteins were quantified through immunohistochemistry and immunoblotting.Androgen receptor target genes were examined by quantitative real-time polymerase chain reaction.The sperm count and body weight of rats were also measured.Results:The oral administration of R.vomitoria extract significantly reduced the prostate weight and prostate weight index in BPH rats,supported by the decreased thickness of the prostate epithelial layer and increased lumen size.Similar effects were observed in the BPH rats treated with the reference drug,finasteride.R.vomitoria extract significantly reduced the testosterone-induced proliferation markers,including proliferating cell nuclear antigen and cyclin D1,in the prostate glands of BPH rats;it also reduced levels of androgen receptor,its associated protein steroid 5 a-reductase 1 and its downstream target genes(FK506-binding protein 5 and matrix metalloproteinase 2).Notably,compared with the finasteride group,R.vomitoria extract did not significantly reduce sperm count.Conclusion:R.vomitoria suppresses testosterone-induced BPH development.Due to its milder side effects,R.vomitoria could be a promising therapeutic agent for BPH.展开更多
从催吐萝芙木中分离到1株对香蕉炭疽菌拮抗作用较强的菌株LYM3,皿内拮抗活性达到64%,通过常规鉴定结合16S r DNA序列分析,将该菌株鉴定为枯草芽孢杆菌(Bacillus subtilis)。通过单因素法对其产抗菌活性物质的发酵条件进行了优化,获得其...从催吐萝芙木中分离到1株对香蕉炭疽菌拮抗作用较强的菌株LYM3,皿内拮抗活性达到64%,通过常规鉴定结合16S r DNA序列分析,将该菌株鉴定为枯草芽孢杆菌(Bacillus subtilis)。通过单因素法对其产抗菌活性物质的发酵条件进行了优化,获得其最适培养基组成为:可溶性淀粉2%,胰蛋白胨1%,酵母粉0.8%,Na Cl 1%,初始p H 6.0、28℃、180 r/min发酵培养60 h,抗菌活性物质产量达到最高,抑菌圈直径为3.42cm。展开更多
基金supported by The Beljanski Foundation(to JY)and Military Laboratory Animal Fund(Grant No.SYDW[2017]15to TF)。
文摘Objective:Herbal medicine is an important therapeutic option for benign prostatic hyperplasia(BPH),a common disease in older men that can seriously affect their quality of life.Currently,it is crucial to develop agents with strong efficacy and few side effects.Herein we investigated the effects of the extract of Rauwolfia vomitoria,a shrub grown in West Africa,on BPH.Methods:Rats with testosterone-induced BPH were treated with R.vomitoria.Prostates were histologically analyzed by Hematoxylin and eosin staining.Proliferation index and the expression levels of androgen receptor and its associated proteins were quantified through immunohistochemistry and immunoblotting.Androgen receptor target genes were examined by quantitative real-time polymerase chain reaction.The sperm count and body weight of rats were also measured.Results:The oral administration of R.vomitoria extract significantly reduced the prostate weight and prostate weight index in BPH rats,supported by the decreased thickness of the prostate epithelial layer and increased lumen size.Similar effects were observed in the BPH rats treated with the reference drug,finasteride.R.vomitoria extract significantly reduced the testosterone-induced proliferation markers,including proliferating cell nuclear antigen and cyclin D1,in the prostate glands of BPH rats;it also reduced levels of androgen receptor,its associated protein steroid 5 a-reductase 1 and its downstream target genes(FK506-binding protein 5 and matrix metalloproteinase 2).Notably,compared with the finasteride group,R.vomitoria extract did not significantly reduce sperm count.Conclusion:R.vomitoria suppresses testosterone-induced BPH development.Due to its milder side effects,R.vomitoria could be a promising therapeutic agent for BPH.
文摘从催吐萝芙木中分离到1株对香蕉炭疽菌拮抗作用较强的菌株LYM3,皿内拮抗活性达到64%,通过常规鉴定结合16S r DNA序列分析,将该菌株鉴定为枯草芽孢杆菌(Bacillus subtilis)。通过单因素法对其产抗菌活性物质的发酵条件进行了优化,获得其最适培养基组成为:可溶性淀粉2%,胰蛋白胨1%,酵母粉0.8%,Na Cl 1%,初始p H 6.0、28℃、180 r/min发酵培养60 h,抗菌活性物质产量达到最高,抑菌圈直径为3.42cm。
