The basidiomycetes Stereum hirsutum and Boreostereum vibrans are two fungi of the same genus.In this study,chemical investigation on the co-cultures of the two congeneric fungi led to the isolation of eleven new vibra...The basidiomycetes Stereum hirsutum and Boreostereum vibrans are two fungi of the same genus.In this study,chemical investigation on the co-cultures of the two congeneric fungi led to the isolation of eleven new vibralactone derivatives,hirsutavibrins A-K(1-11).The structures of 1-11 were elucidated by extensive NMR and HRESIMS spectroscopic analysis,and computational methods.Hirsutavibrins A(1)and B(2)showed weak cytotoxicity against the human lung cancer cell line A549.Hirsutavibrin D(4)showed moderate anti-nitric oxide activity in murine monocytic RAW 264.7 macrophages.This work not only expands the members of vibralactone derivatives with variable configurations but also opens a new avenue for fungal co-culturing study between congeneric fungi.展开更多
Phytochemical reinvestigation on the cultural broth of Boreostereum vibrans led to the isolation of six new vibralactone derivatives,vibralactone N(1),vibralactone O(2),vibralactone P(3),10-lactyl vibralactone G(4),(3...Phytochemical reinvestigation on the cultural broth of Boreostereum vibrans led to the isolation of six new vibralactone derivatives,vibralactone N(1),vibralactone O(2),vibralactone P(3),10-lactyl vibralactone G(4),(3S*,4R*)-6-acetoxymethyl-2,2-dimethyl-3,4-dihydro-2H-chromene-3,4-diol(5),vibralactone Q(6).Their structures were elucidated by extensive spectroscopic methods.展开更多
A scale-up fermentation of the fungus Boreostereum vibrans facilitated the isolation of six new vibralactone biogenesis-associated analogues,namely vibralactamide A(1),vibralactone T(2),13-O-lactyl vibralactone(3),10-...A scale-up fermentation of the fungus Boreostereum vibrans facilitated the isolation of six new vibralactone biogenesis-associated analogues,namely vibralactamide A(1),vibralactone T(2),13-O-lactyl vibralactone(3),10-O-acetyl vibralactone G(4),(11R,12R)-and(11S,12R)-vibradiol(5,6).Their structures were established via extensive spectroscopic analyses,specific optical rotation comparison,and Snatzke’s method.The biosynthetic pathway for vibralactamide A was postulated.The absolute configuration of vibralactone B was revised by single crystal X-ray diffraction analysis.This work puts the divergent vibralactone biosynthesis pathway one step further and expands the structural diversity of vibralactone-associated compounds.展开更多
Seven cadinane sesquiterpenoids,named boreovibrins A-G(1-7),three dihydrobenzofurans(8-10),and two lactones(11 and 12),together with one known compound(13),were isolated from cultures of the basidiomycete Boreostereum...Seven cadinane sesquiterpenoids,named boreovibrins A-G(1-7),three dihydrobenzofurans(8-10),and two lactones(11 and 12),together with one known compound(13),were isolated from cultures of the basidiomycete Boreostereum vibrans.The new structures were elucidated by means of spectroscopic methods.Compounds 5,6,and 11 showed weak inhibitory activities against isozymes of 11β-hydroxysteroid dehydrogenases(11β-HSD).展开更多
基金supported by the National Natural Science Foundation of China(Grant number 82473810)the Fundamental Research Funds for the Central Universities,South-Central Minzu University(Grant No.CPT22033).
文摘The basidiomycetes Stereum hirsutum and Boreostereum vibrans are two fungi of the same genus.In this study,chemical investigation on the co-cultures of the two congeneric fungi led to the isolation of eleven new vibralactone derivatives,hirsutavibrins A-K(1-11).The structures of 1-11 were elucidated by extensive NMR and HRESIMS spectroscopic analysis,and computational methods.Hirsutavibrins A(1)and B(2)showed weak cytotoxicity against the human lung cancer cell line A549.Hirsutavibrin D(4)showed moderate anti-nitric oxide activity in murine monocytic RAW 264.7 macrophages.This work not only expands the members of vibralactone derivatives with variable configurations but also opens a new avenue for fungal co-culturing study between congeneric fungi.
基金This work was financially supported by National Natural Science Foundation of China(81102348).
文摘Phytochemical reinvestigation on the cultural broth of Boreostereum vibrans led to the isolation of six new vibralactone derivatives,vibralactone N(1),vibralactone O(2),vibralactone P(3),10-lactyl vibralactone G(4),(3S*,4R*)-6-acetoxymethyl-2,2-dimethyl-3,4-dihydro-2H-chromene-3,4-diol(5),vibralactone Q(6).Their structures were elucidated by extensive spectroscopic methods.
基金supported by National Natural Science Foundation of China(No.81561148013).
文摘A scale-up fermentation of the fungus Boreostereum vibrans facilitated the isolation of six new vibralactone biogenesis-associated analogues,namely vibralactamide A(1),vibralactone T(2),13-O-lactyl vibralactone(3),10-O-acetyl vibralactone G(4),(11R,12R)-and(11S,12R)-vibradiol(5,6).Their structures were established via extensive spectroscopic analyses,specific optical rotation comparison,and Snatzke’s method.The biosynthetic pathway for vibralactamide A was postulated.The absolute configuration of vibralactone B was revised by single crystal X-ray diffraction analysis.This work puts the divergent vibralactone biosynthesis pathway one step further and expands the structural diversity of vibralactone-associated compounds.
基金This work was financially supported by National Basic Research Program of China(973 Program,2009CB522300)the National Natural Science Foundation of China(30830113,U1132607).
文摘Seven cadinane sesquiterpenoids,named boreovibrins A-G(1-7),three dihydrobenzofurans(8-10),and two lactones(11 and 12),together with one known compound(13),were isolated from cultures of the basidiomycete Boreostereum vibrans.The new structures were elucidated by means of spectroscopic methods.Compounds 5,6,and 11 showed weak inhibitory activities against isozymes of 11β-hydroxysteroid dehydrogenases(11β-HSD).
