Since the beginning of the 21st century,modern medical technology has advanced rapidly,and the cryomedicine has also seen significant progress.Notable developments include the application of cryomedicine in assisted r...Since the beginning of the 21st century,modern medical technology has advanced rapidly,and the cryomedicine has also seen significant progress.Notable developments include the application of cryomedicine in assisted reproduction and the cryopreservation of sperm,eggs and embryos,as well as the preservation of skin,fingers,and other isolated tissues.However,cryopreservation of large and complex tissues or organs remains highly challenging.In addition to the damage caused by the freezing and rewarming processes and the inherent complexity of tissues and organs,there is an urgent need to address issues related to damage detection and the investigation of injury mechanisms.It provides a retrospective analysis of existing methods for assessing tissue and organ viability.Although current techniques can detect damage to some extent,they tend to be relatively simple,time-consuming,and limited in their ability to provide timely and comprehensive assessments of viability.By summarizing and evaluating these approaches,our study aims to contribute to the improvement of viability detection methods and to promote further development in this critical area.展开更多
Anastasis is a phenomenon described as a cellular escape from ethanol-induced cell death.Although the relevant mechanism has not yet been fully elucidated,anastasis is thought to play a role in drug resistance in canc...Anastasis is a phenomenon described as a cellular escape from ethanol-induced cell death.Although the relevant mechanism has not yet been fully elucidated,anastasis is thought to play a role in drug resistance in cancer cells.To date,the regulation of anastasis in normal and cancerous cells has not been clarified.展开更多
The arginine-modified and europium-doped hydroxyapatite nanoparticles（HAP-Eu） were synthesized by hydrothermal synthesis.The prepared nanoparticles were characterized by transmission electron microscopy（TEM）,X-ray...The arginine-modified and europium-doped hydroxyapatite nanoparticles（HAP-Eu） were synthesized by hydrothermal synthesis.The prepared nanoparticles were characterized by transmission electron microscopy（TEM）,X-ray diffractometry（XRD）,Fourier transform infrared（FTIR） and zeta potential analyzer.The cell viability of HAP-Eu was tested by image flow cytometry.The results indicated that HAP-Eu is short column shapes and its size is approximately 100 nm,its zeta potential is about 30.10 mV at pH of 7.5,and shows no cytotoxicity in human epithelial cells and endothelial cells.展开更多
53 rice germplasm resources warehoused during 1981-1984 were regarded as materials to monitor the viability at warehouse time and different years after warehoused. The results showed that seed germination rates of dif...53 rice germplasm resources warehoused during 1981-1984 were regarded as materials to monitor the viability at warehouse time and different years after warehoused. The results showed that seed germination rates of different rice germplasm resources assumed descending trend in storage, with annual decreasing rate between 0.12%-3.05% ; the seed germination rates of most cultivars were above 75% after stored for 26 years; forecasting analysis based on the germination rate of 75% as reference showed a huge difference of safe storage life for different rice germplasm resources, ranging from 12 to 50 years, even longer time. The results suggest that track monitoring on viability and regeneration of rice cultivars is of great importance for germplasm resources conservation.展开更多
The pollen of two tomato varieties, Ryau961721 and Ryau9327D, was adopted in our research. The two tomato varieties were bred by College of Land- scape and Horticulture, Yunnan Agricultural University. The collected p...The pollen of two tomato varieties, Ryau961721 and Ryau9327D, was adopted in our research. The two tomato varieties were bred by College of Land- scape and Horticulture, Yunnan Agricultural University. The collected pollen was stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circumstances. Then it was inoculated to the medium and cultured at 28 ℃ in thermostat incubator. The pollen viability was determined by electron microscope. The results showed that compared to that of pollen stored in control （25 ℃） circumstance, the viability of pollen stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circum- stances for 1 -3 d did not change significantly. In addition, pollen viability trended to decrease with the increase of freeze-thaw cycle and storage time. The pollen lost basically the viability by the 7th d in the storage.展开更多
[Objective] The primary aims of this study were to understand the characteristic of floralorgan development in ‘Dongxing＇, and analyze its differences with other apricot varieties. [Method] Floral organ morphology, ...[Objective] The primary aims of this study were to understand the characteristic of floralorgan development in ‘Dongxing＇, and analyze its differences with other apricot varieties. [Method] Floral organ morphology, pollen quantity, pollen viability and stigma receptivityof ‘Dongxing＇ and other apricot varieties were observed and measured. [Result] The ratio between the medium style and long style of‘Dongxing＇ was 82.4%, and the ratio of pistillode was lower than that in the other apricot varieties. Its pollen quantity was 32 183.2 grains per flower, and was fewer than the others. The pollen germination of ‘Dongxing＇ in optimum medium（1% agar ＋15% sucrose ＋ 0.01% boric acid） was 53.0%, i.e. its pollen viability was low. The pollen viability increased at first and then decreased following the period of the bud stage to flowering stage, and pollen viability reached maximum value at the 1st d after flowering. Meanwhile the stored pollen viability continuously decreased with the extension of storage time, and pollen viability of the both vivo or vitro stored was decreased to the half of the maximum value at the 3rdd after flowering or storage.The stigma receptivity of ‘ongxing＇ enhanced at first then weakened, maximum stigma receptivity was found at the period of 2 h to 4 h after flowering. The optimum time for artificial pollination was 1 h to 8 h after flowering and it was shorter than the other apricot varieties was. [Conclusion] The ratio between the medium style and long style of ‘Dongxing＇ was higher than the other apricot varieties, while the pollen viability of ‘Dongxing＇ on the contrary. Its pollen viability reached maximum value at the 1st d after flowering and the optimum time for artificial pollination was1 h to 8h after flowering.展开更多
Objective To evaluate short time effects of primary percutaneous coronary intervention (pPCI) and rtPA thrombolysis+PCI (rtPA+PCI) on myocardial viability and ventricular systolic synchrony in AMI patients.Methods Eig...Objective To evaluate short time effects of primary percutaneous coronary intervention (pPCI) and rtPA thrombolysis+PCI (rtPA+PCI) on myocardial viability and ventricular systolic synchrony in AMI patients.Methods Eighty seven patients with first AMI were divided into two groups: group A ( n =42), pPCI group, the patients underwent PCI within 6h after onset of AMI; group B ( n =45), rtPA+PCI group, the patients underwent PCI after thrombolysis within 6h after onset of AMI; Myocardial viability was measured by 99m Tc MIBI SPECT. While, the parameters of cardiac function LVEF and ventricular systolic synchrony LVPS were measured by 99m Tc gated cardiac blood pool image on the first and the fourth weekend. Results (1) The peak CK MB was significantly lower in group A than that in group B( P <0.01 ). (2) Myocardial infarction area (MIA) was decreased and radioactivity counts in MIA was significantly increased in group A and B on the 4th weekend compared with that on the first weekend ( P <0.01 ), but there were no significant difference between group A and group B. (3) LVEF, LVPS were no significant difference between group A and group B.Conclusions (1)pPCI in acute myocardial infartion can limit infarct area, maintain ventricular systolic synchrony and improve ventricular function; (2) but, in those hospitals that there were no any condition for PCI, they should transfer the patients to central hospital for PCI after thrombolysis at the first time. It is beneficial to improve myocardial viability and ventricular systolic synchrony of AMI patients in short time.展开更多
Recycling spent lithium-ion(Li+)batteries is critical for achieving environmental conservation and the strategic recovery of essential resources.Compared with conventional methods for recovering cathode materials,whic...Recycling spent lithium-ion(Li+)batteries is critical for achieving environmental conservation and the strategic recovery of essential resources.Compared with conventional methods for recovering cathode materials,which are energy-intensive and prone to secondary pollution,the direct regeneration approach has emerged as a rapid and highly efficient method,gaining widespread attention in recent years.However,this approach faces major challenges,including degraded electrochemical performances and limited economic value.This study,therefore,proposes a high-value direct regeneration strategy to convert degraded spent LiFePO_(4)(S-LFP)into a gradient manganese(Mn)-doped regenerated LiFe_(0.7)Mn_(0.3)PO_(4)/C(R-LFMP)composite.This method leverages the inherent microcracks and Li vacancies present in S-LFP,likely acting as diffusion channels for the Mn^(2+)/Li^(+)ions.Through a two-step mechanochemical ball-milling and carbothermal reduction process,this approach achieves simultaneous Li replenishment and surface-localised Mn gradient doping with enhanced structural control.Notably,the R-LFMP exhibits an exceptional electrochemical performance.At 0.1 C,it delivers a discharge capacity of 161.4 mA h g^(−1)and an energy density of 563.5 Wh kg^(−1)(representing a 60.5%improvement over S-LFP).Additionally,it maintains 83%capacity retention after 900 cycles at 0.5C,a considerable enhancement compared to commercial LFMP(62%).Furthermore,the regenerated cathode material generates a net profit of$7.102 kg^(−1),surpassing the profitability of conventional recycling methods by 90%.Overall,this study introduces a transformative and sustainable LFP regeneration technology,achieving breakthroughs in electrochemical restoration and high-value recycling,while paving the way for the closed-loop utilisation of LFP-based energy storage systems.展开更多
In Indonesia, shallot cultivation finds difficulties to produce high viability seed. That low viability is caused by the abnormality of seed＇s structure. Normal shallot seeds have seed structure that consists of： te...In Indonesia, shallot cultivation finds difficulties to produce high viability seed. That low viability is caused by the abnormality of seed＇s structure. Normal shallot seeds have seed structure that consists of： testa, endosperm, and embryo. Normal embryo has curved shape which it has one cotyledon in endosperm. This research purpose is to find shallot viability and structure that is cultivated in tropic area, especially in Indonesia. This research is a deep observation research about water level, seeds viability and seeds structure monitoring by using microscope. To make a clearance about seeds structure composition, seeds are mixed with tetrazolium salt. Monitoring result shows that seed water level at harvest time is 18%, laid away at one month age is 13%, two months age is 12% and three months age is 10%. Seed viability at harvest until twenty nine days, seed that is germinated around 8%. At two months viability seed laid age until twenty two days is 36.6%. Meanwhile three months age laid viability test is done with germination boost compounding shows that IAA is able to fasten germination. At IAA treatment viability seeds are 17.25% that is followed with GA3 treatment around 13%. Another boosting substance that it＇s viability is only below 4%. Low viability is also proved by the seed structure. The result shows that mostly, the seeds has abnormal structure.展开更多
This paper presents an economic viability analysis of two investment alternatives for a pig farming located in the city of Jatai, Goias State, Brazil. The first investment alternative consists of the implementation of...This paper presents an economic viability analysis of two investment alternatives for a pig farming located in the city of Jatai, Goias State, Brazil. The first investment alternative consists of the implementation of a biodigester, being the biogas burned in a flare. The second alternative also consists of a biodigester, nevertheless the produced biogas is used to generate electricity. The methodologies to estimate the methane production in anaerobic reactors are presented, as well as those for the quantification of the baseline and project emissions and of the electricity that can be generated. The results showed that the use of biogas for the electricity generation is the alternative more economically viable.展开更多
文摘Since the beginning of the 21st century,modern medical technology has advanced rapidly,and the cryomedicine has also seen significant progress.Notable developments include the application of cryomedicine in assisted reproduction and the cryopreservation of sperm,eggs and embryos,as well as the preservation of skin,fingers,and other isolated tissues.However,cryopreservation of large and complex tissues or organs remains highly challenging.In addition to the damage caused by the freezing and rewarming processes and the inherent complexity of tissues and organs,there is an urgent need to address issues related to damage detection and the investigation of injury mechanisms.It provides a retrospective analysis of existing methods for assessing tissue and organ viability.Although current techniques can detect damage to some extent,they tend to be relatively simple,time-consuming,and limited in their ability to provide timely and comprehensive assessments of viability.By summarizing and evaluating these approaches,our study aims to contribute to the improvement of viability detection methods and to promote further development in this critical area.
基金supported by the Karadeniz Technical University(Nos.FBA-2018-7951 and FYL-2019-8116)the Scientific and Technological Research Council of Türkiye(TUBITAK)(No.123Z002).
文摘Anastasis is a phenomenon described as a cellular escape from ethanol-induced cell death.Although the relevant mechanism has not yet been fully elucidated,anastasis is thought to play a role in drug resistance in cancer cells.To date,the regulation of anastasis in normal and cancerous cells has not been clarified.
基金Project (81071869) supported by the National Natural Science Foundation of China Project (2009637526) supported by China Scholarship Council (CSC Program)Project (2010QZZD006) supported by the Key Program of Central South University Advancing Front Foundation
文摘The arginine-modified and europium-doped hydroxyapatite nanoparticles（HAP-Eu） were synthesized by hydrothermal synthesis.The prepared nanoparticles were characterized by transmission electron microscopy（TEM）,X-ray diffractometry（XRD）,Fourier transform infrared（FTIR） and zeta potential analyzer.The cell viability of HAP-Eu was tested by image flow cytometry.The results indicated that HAP-Eu is short column shapes and its size is approximately 100 nm,its zeta potential is about 30.10 mV at pH of 7.5,and shows no cytotoxicity in human epithelial cells and endothelial cells.
文摘53 rice germplasm resources warehoused during 1981-1984 were regarded as materials to monitor the viability at warehouse time and different years after warehoused. The results showed that seed germination rates of different rice germplasm resources assumed descending trend in storage, with annual decreasing rate between 0.12%-3.05% ; the seed germination rates of most cultivars were above 75% after stored for 26 years; forecasting analysis based on the germination rate of 75% as reference showed a huge difference of safe storage life for different rice germplasm resources, ranging from 12 to 50 years, even longer time. The results suggest that track monitoring on viability and regeneration of rice cultivars is of great importance for germplasm resources conservation.
基金Supported by College Students’Technology Innovation and Entrepreneurship Action Fund of Yunnan Agricultural University(2014ZKX048)~~
文摘The pollen of two tomato varieties, Ryau961721 and Ryau9327D, was adopted in our research. The two tomato varieties were bred by College of Land- scape and Horticulture, Yunnan Agricultural University. The collected pollen was stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circumstances. Then it was inoculated to the medium and cultured at 28 ℃ in thermostat incubator. The pollen viability was determined by electron microscope. The results showed that compared to that of pollen stored in control （25 ℃） circumstance, the viability of pollen stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circum- stances for 1 -3 d did not change significantly. In addition, pollen viability trended to decrease with the increase of freeze-thaw cycle and storage time. The pollen lost basically the viability by the 7th d in the storage.
基金Supported by Xinjiang Uygur Nationality Autonomous Region Science and Technology Project(201130102-1)Xinjiang Uygur Nationality Autonomous Region Key Subjects of Pomology~~
文摘[Objective] The primary aims of this study were to understand the characteristic of floralorgan development in ‘Dongxing＇, and analyze its differences with other apricot varieties. [Method] Floral organ morphology, pollen quantity, pollen viability and stigma receptivityof ‘Dongxing＇ and other apricot varieties were observed and measured. [Result] The ratio between the medium style and long style of‘Dongxing＇ was 82.4%, and the ratio of pistillode was lower than that in the other apricot varieties. Its pollen quantity was 32 183.2 grains per flower, and was fewer than the others. The pollen germination of ‘Dongxing＇ in optimum medium（1% agar ＋15% sucrose ＋ 0.01% boric acid） was 53.0%, i.e. its pollen viability was low. The pollen viability increased at first and then decreased following the period of the bud stage to flowering stage, and pollen viability reached maximum value at the 1st d after flowering. Meanwhile the stored pollen viability continuously decreased with the extension of storage time, and pollen viability of the both vivo or vitro stored was decreased to the half of the maximum value at the 3rdd after flowering or storage.The stigma receptivity of ‘ongxing＇ enhanced at first then weakened, maximum stigma receptivity was found at the period of 2 h to 4 h after flowering. The optimum time for artificial pollination was 1 h to 8 h after flowering and it was shorter than the other apricot varieties was. [Conclusion] The ratio between the medium style and long style of ‘Dongxing＇ was higher than the other apricot varieties, while the pollen viability of ‘Dongxing＇ on the contrary. Its pollen viability reached maximum value at the 1st d after flowering and the optimum time for artificial pollination was1 h to 8h after flowering.
文摘Objective To evaluate short time effects of primary percutaneous coronary intervention (pPCI) and rtPA thrombolysis+PCI (rtPA+PCI) on myocardial viability and ventricular systolic synchrony in AMI patients.Methods Eighty seven patients with first AMI were divided into two groups: group A ( n =42), pPCI group, the patients underwent PCI within 6h after onset of AMI; group B ( n =45), rtPA+PCI group, the patients underwent PCI after thrombolysis within 6h after onset of AMI; Myocardial viability was measured by 99m Tc MIBI SPECT. While, the parameters of cardiac function LVEF and ventricular systolic synchrony LVPS were measured by 99m Tc gated cardiac blood pool image on the first and the fourth weekend. Results (1) The peak CK MB was significantly lower in group A than that in group B( P <0.01 ). (2) Myocardial infarction area (MIA) was decreased and radioactivity counts in MIA was significantly increased in group A and B on the 4th weekend compared with that on the first weekend ( P <0.01 ), but there were no significant difference between group A and group B. (3) LVEF, LVPS were no significant difference between group A and group B.Conclusions (1)pPCI in acute myocardial infartion can limit infarct area, maintain ventricular systolic synchrony and improve ventricular function; (2) but, in those hospitals that there were no any condition for PCI, they should transfer the patients to central hospital for PCI after thrombolysis at the first time. It is beneficial to improve myocardial viability and ventricular systolic synchrony of AMI patients in short time.
基金supported by the National Key Research and Development Program of China(2023YFB3809300).
文摘Recycling spent lithium-ion(Li+)batteries is critical for achieving environmental conservation and the strategic recovery of essential resources.Compared with conventional methods for recovering cathode materials,which are energy-intensive and prone to secondary pollution,the direct regeneration approach has emerged as a rapid and highly efficient method,gaining widespread attention in recent years.However,this approach faces major challenges,including degraded electrochemical performances and limited economic value.This study,therefore,proposes a high-value direct regeneration strategy to convert degraded spent LiFePO_(4)(S-LFP)into a gradient manganese(Mn)-doped regenerated LiFe_(0.7)Mn_(0.3)PO_(4)/C(R-LFMP)composite.This method leverages the inherent microcracks and Li vacancies present in S-LFP,likely acting as diffusion channels for the Mn^(2+)/Li^(+)ions.Through a two-step mechanochemical ball-milling and carbothermal reduction process,this approach achieves simultaneous Li replenishment and surface-localised Mn gradient doping with enhanced structural control.Notably,the R-LFMP exhibits an exceptional electrochemical performance.At 0.1 C,it delivers a discharge capacity of 161.4 mA h g^(−1)and an energy density of 563.5 Wh kg^(−1)(representing a 60.5%improvement over S-LFP).Additionally,it maintains 83%capacity retention after 900 cycles at 0.5C,a considerable enhancement compared to commercial LFMP(62%).Furthermore,the regenerated cathode material generates a net profit of$7.102 kg^(−1),surpassing the profitability of conventional recycling methods by 90%.Overall,this study introduces a transformative and sustainable LFP regeneration technology,achieving breakthroughs in electrochemical restoration and high-value recycling,while paving the way for the closed-loop utilisation of LFP-based energy storage systems.
文摘In Indonesia, shallot cultivation finds difficulties to produce high viability seed. That low viability is caused by the abnormality of seed＇s structure. Normal shallot seeds have seed structure that consists of： testa, endosperm, and embryo. Normal embryo has curved shape which it has one cotyledon in endosperm. This research purpose is to find shallot viability and structure that is cultivated in tropic area, especially in Indonesia. This research is a deep observation research about water level, seeds viability and seeds structure monitoring by using microscope. To make a clearance about seeds structure composition, seeds are mixed with tetrazolium salt. Monitoring result shows that seed water level at harvest time is 18%, laid away at one month age is 13%, two months age is 12% and three months age is 10%. Seed viability at harvest until twenty nine days, seed that is germinated around 8%. At two months viability seed laid age until twenty two days is 36.6%. Meanwhile three months age laid viability test is done with germination boost compounding shows that IAA is able to fasten germination. At IAA treatment viability seeds are 17.25% that is followed with GA3 treatment around 13%. Another boosting substance that it＇s viability is only below 4%. Low viability is also proved by the seed structure. The result shows that mostly, the seeds has abnormal structure.
文摘This paper presents an economic viability analysis of two investment alternatives for a pig farming located in the city of Jatai, Goias State, Brazil. The first investment alternative consists of the implementation of a biodigester, being the biogas burned in a flare. The second alternative also consists of a biodigester, nevertheless the produced biogas is used to generate electricity. The methodologies to estimate the methane production in anaerobic reactors are presented, as well as those for the quantification of the baseline and project emissions and of the electricity that can be generated. The results showed that the use of biogas for the electricity generation is the alternative more economically viable.
