In recent years,the problem of veterinary drug residues in animal-derived foods has attracted worldwide attention.Developing rapid,simple,highly sensitive,and high-throughput veterinary drug residue detection technolo...In recent years,the problem of veterinary drug residues in animal-derived foods has attracted worldwide attention.Developing rapid,simple,highly sensitive,and high-throughput veterinary drug residue detection technologies has become an urgent need.This paper provides a comprehensive review of the pretreatment and analytical techniques for veterinary drug residue analysis,comparing the detection principles,operational procedures,and respective advantages and disadvantages of various detection technologies.It further explores the future development directions of veterinary drug residue detection technologies.展开更多
Scopus recently releasedits 2024CiteScore metrics.The 2024 CiteScore for Animal Diseases is 3.6,representing a 50%increase compared to 2023.The journal's ranking has risen significantly:It ranks 38th out of 200 jo...Scopus recently releasedits 2024CiteScore metrics.The 2024 CiteScore for Animal Diseases is 3.6,representing a 50%increase compared to 2023.The journal's ranking has risen significantly:It ranks 38th out of 200 journals in the Veterinary category,marking the 81%and placing it in the Q1 zone.Simultaneously,it ranks 110th out of 509 journals in the Animal Science and Zoology category,placing it at the 78%and also within the Q1 zone.展开更多
[Objective]To screen sensitive drugs and their combinations against biofilm formation of Escherichia coli isolated from Tibetan pigs and Tibetan chickens.[Method]Semi-quantitative modified crystal violet staining and ...[Objective]To screen sensitive drugs and their combinations against biofilm formation of Escherichia coli isolated from Tibetan pigs and Tibetan chickens.[Method]Semi-quantitative modified crystal violet staining and the micro broth dilution method were employed to assess the biofilm-forming capacities of 152 E.coli,and their susceptibility to 12 commonly used antibiotics.Meanwhile,the checkerboard testing method was employed to evaluate the combined antibacterial activity of the sequential solvent fractions(methanol,dichloromethane,ethyl acetate,and petroleum ether)from four Tibetan veterinary medicinal plants(Spenceria ramalana Trimen,Thalictrum delawayi Franch.,Gentiana sino-ornata Balf.f.,Lonicera rupicola Hook.f.&Thomson),the ethyl acetate extract of two Enterococcus faecium and antibiotics with high resistance rates[chloramphenicol(CHL),oxytetracycline(OTC),ampicillin(AMP),and sulfamethoxazole(SMZ)]against ten selected multi-drug resistant E.coli(designated E1-E10).Lastly,the biofilm eradication of drug combinations on biofilms of E.coli E6 was examined.[Result]Most of the 152 E.coli exhibited moderate and no biofilm-forming capacities.The resistance rates to CHL,sulfamethazine(SM2),OTC,AMP,and SMZ exceeded 90%.In contrast,the resistance rates to difloxacin(DIF),ciprofloxacin(CIP),amikacin(AMK),ceftiofur(EFT),tobramycin(TOB),and ceftriaxone(CTR)were below 50%.Notably,the strains demonstrated a higher susceptibility to amikacin,with a drug resistance rate of only 19.90%.The MIC values for the E.coli E1-E10 were observed to range from 3.93 to 15.63 mg/mL for the methanol extract of S.ramalana,3.93 to 31.52 mg/mL for the ethyl acetate extract of T.delavayi,7.81 to 15.63 mg/mL for the methanol extract of G.sino-ornata,and 7.81 to 31.52 mg/mL for the ethyl acetate extract of L.rupicola.The ethyl acetate extracts from E.aecium S16 and S17 exhibited MIC values ranging from 0.42 to 13.38 mg/mL and from 0.45 to 3.63 mg/mL,respectively.The combinations of sequential solvent fractions derived from four Tibetan veterinary medicinal plants,the ethyl acetate extract of E.faecium,and antibiotics exhibiting high resistance rates demonstrated varying effects.At the MIC,all drug combinations showed a significant biofilm eradication effect compared to their single applications.The ethyl acetate extracts of L.rupicola and T.delavayi,combined with OTC and AMP,demonstrated a stronger inhibitory effect on E.coli E6 biofilm compared to other combinations.Specifically,the ethyl acetate extracts of L.rupicola and T.delavayi inhibited E.coli E6 biofilm by 48.92%and 42.58%in the presence of OTC,and by 48.58%and 47.84%in the presence of AMP,respectively.[Conclusion]The combined application of four selected Tibetan medicinal plants,probiotics,and antibacterial agents may offer a potential solution to address drug resistance and biofilm formation in E.coli isolated from Tibetan pigs and Tibetan chickens.展开更多
[Objective] This study aimed to investigate the treatment efficacy of tradi- tional Chinese veterinary medicine mycoplasma gatlisepticum infection spray against experimental mycoplasma gallisepticum infection. [Method...[Objective] This study aimed to investigate the treatment efficacy of tradi- tional Chinese veterinary medicine mycoplasma gatlisepticum infection spray against experimental mycoplasma gallisepticum infection. [Method] Experimental chickens in- fected with mycoplasma gallisepticum were used as models to investigate the treat- ment efficacy of different doses of traditional Chinese veterinary medicine mycoplas- ma gallisepticum infection spray. [Result] Medication with traditional Chinese veteri- nary medicine mycoplasma gallisepticum infection spray for continuous 5 d showed certain treatment efficacy against mycoplasma gallisepticum infection. The relative weight gain rate of 0.5 and 1.0 g/m3 dose groups was significantly higher than the infection control group （P〈0.01）; the cure rate of mycoplasma gallisepticum infection spray groups was significantly higher than the infection control group （P〈0.01） and showed no significant difference compared with Taimiaoling control group （P〉0.05）; the mortality rate showed no significant difference among various groups （P〉0.05）, indicating that traditional Chinese veterinary medicine mycoplasma gallisepticum in- fection spray has relatively ideal treatment efficacy against mycoplasma gallisepticum infection. [Conclusion] This study provided reference for treatment of chickens infect- ed with mycoplasma gallisepticum.展开更多
With the development of pharmaceutics and other disciplines theories and advanced technologies, the application of many new drug delivery systems has gradually increased in clinical veterinary. Among the many drug del...With the development of pharmaceutics and other disciplines theories and advanced technologies, the application of many new drug delivery systems has gradually increased in clinical veterinary. Among the many drug delivery systems, transdermal patch can maintain stable and effective plasma concentration and therapeutic effect in vivo for a long time after skin dressing delivery, which provides a safe and effective drug-delivery way for the therapy and prevention of some chronic diseases and partial analgesia in a simple and convenient way. Veterinary drug transdermal preparations have been developed both at home and abroad, and satisfactory results have been achieved in the experimental application. Based on the study of veterinary transdermal patches at home and abroad, this paper systematically describes the development and characteristics of transdermal patches, the factors affecting skin permeation and the evaluation this type of preparations in veterinary drugs.展开更多
基金Supported by Tangshan Science and Technology Planning Project(25150202E).
文摘In recent years,the problem of veterinary drug residues in animal-derived foods has attracted worldwide attention.Developing rapid,simple,highly sensitive,and high-throughput veterinary drug residue detection technologies has become an urgent need.This paper provides a comprehensive review of the pretreatment and analytical techniques for veterinary drug residue analysis,comparing the detection principles,operational procedures,and respective advantages and disadvantages of various detection technologies.It further explores the future development directions of veterinary drug residue detection technologies.
文摘Scopus recently releasedits 2024CiteScore metrics.The 2024 CiteScore for Animal Diseases is 3.6,representing a 50%increase compared to 2023.The journal's ranking has risen significantly:It ranks 38th out of 200 journals in the Veterinary category,marking the 81%and placing it in the Q1 zone.Simultaneously,it ranks 110th out of 509 journals in the Animal Science and Zoology category,placing it at the 78%and also within the Q1 zone.
基金Supported by Project of Sichuan Provincial Department of Science and Technology(2016KZ0007)Science and Technology Project of Sichuan Province(2022JDRC0121)+1 种基金Innovative Research Projects for Postgraduate at Southwest Minzu University(CX2020SZ49)Sichuan Provincial Department of Science and Technology(2023NSFSC0179)。
文摘[Objective]To screen sensitive drugs and their combinations against biofilm formation of Escherichia coli isolated from Tibetan pigs and Tibetan chickens.[Method]Semi-quantitative modified crystal violet staining and the micro broth dilution method were employed to assess the biofilm-forming capacities of 152 E.coli,and their susceptibility to 12 commonly used antibiotics.Meanwhile,the checkerboard testing method was employed to evaluate the combined antibacterial activity of the sequential solvent fractions(methanol,dichloromethane,ethyl acetate,and petroleum ether)from four Tibetan veterinary medicinal plants(Spenceria ramalana Trimen,Thalictrum delawayi Franch.,Gentiana sino-ornata Balf.f.,Lonicera rupicola Hook.f.&Thomson),the ethyl acetate extract of two Enterococcus faecium and antibiotics with high resistance rates[chloramphenicol(CHL),oxytetracycline(OTC),ampicillin(AMP),and sulfamethoxazole(SMZ)]against ten selected multi-drug resistant E.coli(designated E1-E10).Lastly,the biofilm eradication of drug combinations on biofilms of E.coli E6 was examined.[Result]Most of the 152 E.coli exhibited moderate and no biofilm-forming capacities.The resistance rates to CHL,sulfamethazine(SM2),OTC,AMP,and SMZ exceeded 90%.In contrast,the resistance rates to difloxacin(DIF),ciprofloxacin(CIP),amikacin(AMK),ceftiofur(EFT),tobramycin(TOB),and ceftriaxone(CTR)were below 50%.Notably,the strains demonstrated a higher susceptibility to amikacin,with a drug resistance rate of only 19.90%.The MIC values for the E.coli E1-E10 were observed to range from 3.93 to 15.63 mg/mL for the methanol extract of S.ramalana,3.93 to 31.52 mg/mL for the ethyl acetate extract of T.delavayi,7.81 to 15.63 mg/mL for the methanol extract of G.sino-ornata,and 7.81 to 31.52 mg/mL for the ethyl acetate extract of L.rupicola.The ethyl acetate extracts from E.aecium S16 and S17 exhibited MIC values ranging from 0.42 to 13.38 mg/mL and from 0.45 to 3.63 mg/mL,respectively.The combinations of sequential solvent fractions derived from four Tibetan veterinary medicinal plants,the ethyl acetate extract of E.faecium,and antibiotics exhibiting high resistance rates demonstrated varying effects.At the MIC,all drug combinations showed a significant biofilm eradication effect compared to their single applications.The ethyl acetate extracts of L.rupicola and T.delavayi,combined with OTC and AMP,demonstrated a stronger inhibitory effect on E.coli E6 biofilm compared to other combinations.Specifically,the ethyl acetate extracts of L.rupicola and T.delavayi inhibited E.coli E6 biofilm by 48.92%and 42.58%in the presence of OTC,and by 48.58%and 47.84%in the presence of AMP,respectively.[Conclusion]The combined application of four selected Tibetan medicinal plants,probiotics,and antibacterial agents may offer a potential solution to address drug resistance and biofilm formation in E.coli isolated from Tibetan pigs and Tibetan chickens.
基金Supported by Project of Hebei Science and Technology Department(08820412D, 12820421D)Project of Shijiazhuang Municipal Science and Technology Bureau (07150193A)PhD Fund of Hebei Normal University of Science and Technology (2007YB002)~~
文摘[Objective] This study aimed to investigate the treatment efficacy of tradi- tional Chinese veterinary medicine mycoplasma gatlisepticum infection spray against experimental mycoplasma gallisepticum infection. [Method] Experimental chickens in- fected with mycoplasma gallisepticum were used as models to investigate the treat- ment efficacy of different doses of traditional Chinese veterinary medicine mycoplas- ma gallisepticum infection spray. [Result] Medication with traditional Chinese veteri- nary medicine mycoplasma gallisepticum infection spray for continuous 5 d showed certain treatment efficacy against mycoplasma gallisepticum infection. The relative weight gain rate of 0.5 and 1.0 g/m3 dose groups was significantly higher than the infection control group （P〈0.01）; the cure rate of mycoplasma gallisepticum infection spray groups was significantly higher than the infection control group （P〈0.01） and showed no significant difference compared with Taimiaoling control group （P〉0.05）; the mortality rate showed no significant difference among various groups （P〉0.05）, indicating that traditional Chinese veterinary medicine mycoplasma gallisepticum in- fection spray has relatively ideal treatment efficacy against mycoplasma gallisepticum infection. [Conclusion] This study provided reference for treatment of chickens infect- ed with mycoplasma gallisepticum.
基金Supported by the Program for Technology Research and Development and Its Demonstration and Popularization of Beijing Vocational College of Agriculture(XY-YF-15-08)~~
文摘With the development of pharmaceutics and other disciplines theories and advanced technologies, the application of many new drug delivery systems has gradually increased in clinical veterinary. Among the many drug delivery systems, transdermal patch can maintain stable and effective plasma concentration and therapeutic effect in vivo for a long time after skin dressing delivery, which provides a safe and effective drug-delivery way for the therapy and prevention of some chronic diseases and partial analgesia in a simple and convenient way. Veterinary drug transdermal preparations have been developed both at home and abroad, and satisfactory results have been achieved in the experimental application. Based on the study of veterinary transdermal patches at home and abroad, this paper systematically describes the development and characteristics of transdermal patches, the factors affecting skin permeation and the evaluation this type of preparations in veterinary drugs.
