BACKGROUND Polycythemia vera(PV)is a myeloproliferative neoplasm characterized by excessive blood cell production,which increases the risk of thrombosis.Ropeginterferon alfa-2b(RI)offers potential advantages over stan...BACKGROUND Polycythemia vera(PV)is a myeloproliferative neoplasm characterized by excessive blood cell production,which increases the risk of thrombosis.Ropeginterferon alfa-2b(RI)offers potential advantages over standard therapy(ST;including phlebotomy,hydroxyurea,and aspirin)by achieving hematologic and molecular responses.However,its comparative efficacy and safety remain understudied.We hypothesized that RI would improve hematologic and molecular outcomes but may differ in safety profiles compared to ST.AIM To evaluate the efficacy and safety of RI vs ST in patients with PV,focusing on hematologic response,molecular response,adverse events(AEs),and thrombotic risk.METHODS This Preferred Reporting Items for Systematic Reviews and Meta-Analyses-compliant meta-analysis included randomized controlled trials comparing RI to ST in adult PV patients.PubMed,EMBASE,ClinicalTrials.gov,and ScienceDirect were searched from inception to July 2025.Outcomes included complete hematological response(CHR),molecular response,AEs leading to discontinuation,JAK2V617F allele burden,thrombotic events,and phlebotomy frequency.Pooled odds ratios(ORs)and MD with 95%confidence intervals(95%CIs)were calculated using random-effects models.Risk of bias was assessed with Cochrane RoB 2;evidence certainty was evaluated via GRADE.RESULTS Five studies involving 477 RI and 456 ST patients were included.RI significantly improved CHR(OR=2.14,95%CI:1.18-3.88,P=0.002)and molecular response(OR=4.37,95%CI:0.99-19.38,P=0.05),with substantial heterogeneity(I²=76%and I²=93%,respectively).AEs leading to discontinuation were higher with RI(OR=3.89,95%CI:1.90-7.97,P=0.0002;I²=0%).No significant differences were observed in JAK2V617F allele burden(MD=-7.46,95%CI:-21.12 to 6.20,P=0.28;I²=90%)or thrombotic events(OR=0.93,95%CI:0.45-1.90,P=0.83;I²=0%).RI reduced phlebotomy frequency(MD=-1.52,95%CI:-2.37 to-0.67,P=0.0005;I²=0%).Most studies had low to moderate risk of bias;evidence certainty was moderate for CHR and AEs,low for molecular response and thrombotic events,and very low for allele burden.CONCLUSION RI offers superior hematologic and molecular responses compared to ST in PV but is associated with higher discontinuation rates due to AEs.Comparable thrombotic risk and reduced phlebotomy needs highlight its potential,though tolerability requires careful management.The high heterogeneity in certain outcomes and potential for publication bias warrant cautious interpretation of these findings.Further long-term studies are needed to optimize dosing and patient selection.展开更多
Thrombosis is a leading cause of mortality and morbidity in patients suffering from polycythemia vera(PV).Drug pair Salviae Miltiorrhizae Radix et Rhizoma(Danshen,DS)and Notoginseng Radix et Rhizoma(Sanqi,SQ)is common...Thrombosis is a leading cause of mortality and morbidity in patients suffering from polycythemia vera(PV).Drug pair Salviae Miltiorrhizae Radix et Rhizoma(Danshen,DS)and Notoginseng Radix et Rhizoma(Sanqi,SQ)is common traditional Chinese medicine(TCM)used in clinical practice to promote blood circulation and eliminate blood stasis.In this study,network pharmacology and molecular docking were used to analyze the potentially active ingredients and underlying mechanisms of drug pair DS-SQ against thrombosis after PV.These results show that 54 targets are related to both disease and the drug pair.Nineteen core targets,including IL-6 and AKT1,were screened.Luteolin and tanshinone IIa from DS as well as quercetin from SQ might be the major substances in the treatment of thrombosis after PV.KEGG enrichment analysis demonstrated that the lipid and atherosclerosis signaling pathway might play a significant role.These results provide valuable insights and a reference for the use of drug pair DS-SQ in management of thrombosis after PV and lay a foundation for further exploration of pharmacological effects.展开更多
Corneal diseases,the second leading cause of global vision loss affecting over 10.5 million people,underscores the unmet demand for corneal tissue replacements.Given the scarcity of fresh donor corneas and the associa...Corneal diseases,the second leading cause of global vision loss affecting over 10.5 million people,underscores the unmet demand for corneal tissue replacements.Given the scarcity of fresh donor corneas and the associated risks of immune rejection,corneal tissue engineering becomes imperative.Developing nanofibrous scaffolds that mimic the natural corneal structure is crucial for creating transparent and mechanically robust corneal equivalents in tissue engineering.Herein,Aloe Vera Extract(AVE)/Polycaprolactone(PCL)nanofibrous scaffolds were primed using electrospinning.The electrospun AVE/PCL fibers exhibit a smooth,bead-free morphology with a mean diameter of approximately 340±95 nm and appropriate light transparency.Mechanical measurements reveal Young’s modulus and ultimate tensile strength values of around 3.34 MPa and 4.58 MPa,respectively,within the range of stromal tissue.In addition,cell viability of AVE/PCL fibers was measured against Human Stromal Keratocyte Cells(HSKCs),and improved cell viability was observed.The cell-fiber interactions were investigated using scanning electron microscopy.In conclusion,the incorporation of Aloe Vera Extract enhances the mechanical,optical,hydrophilic,and biological properties of PCL fibers,positioning PCL/AVE fiber scaffolds as promising candidates for corneal stromal regeneration.展开更多
BACKGROUND The combination of polycythemia vera(PV)with pathological cardiac hypertro-phy is uncommon.In this study,we describe a case of PV accompanied by pa-thological cardiac hypertrophy.It is hypothesized that the...BACKGROUND The combination of polycythemia vera(PV)with pathological cardiac hypertro-phy is uncommon.In this study,we describe a case of PV accompanied by pa-thological cardiac hypertrophy.It is hypothesized that the pronounced cardiac hypertrophy in this patient has a strong connection with PV.CASE SUMMARY In 2021,a 34-year-old Chinese man experienced chest constriction,shortness of breath,and palpitations during vigorous activity.Each episode lasted several minutes and resolved spontaneously following cessation of vigorous activity.He occasionally experienced syncope and vertigo without a headache.He underwent cardiac magnetic resonance imaging and was diagnosed with“hypertrophic cardiomyopathy(HCM)”.He was discharged after receiving symptomatic treat-ment,which resulted in an improvement.He presented to our department with chest constriction,shortness of breath,and respiratory distress for one month while climbing to the second floor in 2023.His blood pressure was 180/100 mmHg at the time of admittance,and he was receiving antihypertensive treat-ment.He had a history of PV for 2 years without treatment.Symptomatic treat-ment was implemented concurrently with the administration of hydroxyurea upon admission.Good blood pressure control was observed during the long-term follow-up,and echocardiography did not reveal any progression of myocardial hypertrophy.CONCLUSION Clinicians managing PV patients should remain highly vigilant regarding the risks of thrombosis and cardio-vascular complications,particularly in those with refractory hypertension.展开更多
Objective: This study compares hemoglobin and erythropoietin levels in patients with polycythemia vera and secondary polycythemia. Study Design: A retrospective cross-sectional study evaluating the serum erythropoieti...Objective: This study compares hemoglobin and erythropoietin levels in patients with polycythemia vera and secondary polycythemia. Study Design: A retrospective cross-sectional study evaluating the serum erythropoietin and hemoglobin levels in patients with polycythemia vera and secondary polycythemia. This study was performed simultaneously in Texas state of the U.S. and Fars Province in Iran. Methods: Hemoglobin, hematocrit and erythropoietin test results were collected from patients aged 19 to 75 years who were diagnosed with polycythemia vera and secondary polycythemia. Patients records with history of thrombocythemia, congestive heart failure, dyspnea, anemia and pregnant woman were excluded from study. Patients in each decade of life were examined in separate groups, so that changes in hemoglobin related to aging did not affect the research results. Results: 75% of the patients were men, and 25% were women. A total of 1580 patients were analyzed in this study. 57.3% of patients in UTMB and 38.8 patients in Iran have hemoglobin level above 17 mg/dl. 74% of patients in UTMB and 88% of patients in Iran have erythropoietin below 10 IU/mL. Polycythemia in UTMB was more common in people over 50 and in Iran in patients under 50 years old. The serum hemoglobin and erythropoietin levels in patients with polycythemia vera were not significantly different in compare to secondary polycythemia patients. Data showed that there were 84 polycythemia patients per 100,000 people. The results of this study in UTMB and Iran showed that 4.5% and 7%, respectively, of patients with polycythemia had a positive JAK2 test. Conclusion: Low erythropoietin levels may not be helpful in differentiating polycythemia vera from secondary polycythemia. .展开更多
Polycythemia vera manifests as a myeloproliferative neoplasm associated with diverse symptoms, including aquagenic pruritis. This systematic review addresses the pressing need to enhance the understanding of the dise...Polycythemia vera manifests as a myeloproliferative neoplasm associated with diverse symptoms, including aquagenic pruritis. This systematic review addresses the pressing need to enhance the understanding of the disease’s symptomatology and optimize treatment strategies for improved patient outcomes. The rarity and low prevalence of polycythemia vera underscore the importance of this investigation, as existing standard of care involves a multifaceted approach and significant healthcare costs. Despite advancement in therapeutic options, persistent symptoms and resistance to first-line treatments pose challenges. Ruxolitinib has emerged as a promising intervention, demonstrating clinically significant improvement for patients. This systematic review appraises three randomized controlled trials, shedding light on the efficacy of ruxolitinib and its potential to ameliorate pruritis symptoms in symptomatic patients.展开更多
The International Union of Geological Sciences(IUGS)is celebrating women and girls in geoscience and in celebrating the 60^(th)anniversary of the IUGS,we must applaud those women who have pioneered the work of the Int...The International Union of Geological Sciences(IUGS)is celebrating women and girls in geoscience and in celebrating the 60^(th)anniversary of the IUGS,we must applaud those women who have pioneered the work of the International Union of Geosciences.Between 1976 to 1980,Prof.Rudolf Trümpy(Switzerland)was President of the IUGS and William Watt Hutchison(Canada),its Secretary General and later President(1984–1987).Hutchison appointed Canadian Vera Lafferty to his Secretariat under the auspices of the Geological Survey of Canada in Ottawa.In this post,she created not only the flagship Episodes magazine but forged a new style across the IUGS platform.Vera Lafferty’s major contributions to the foundation and style of Episodes and the promotion of the IUGS are recognized.展开更多
[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia...[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ＇Kerman＇,tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result]Mediums of 1/2DKW and 1/2DKW＋3.00 mg/L of 6-BA ＋0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio; Proliferation coefficient was 3.6 on the medium of 1/2DKW ＋4.00 mg/L of 6-BA ＋ 0.05 mg/L of IBA; Rooting rate was up to 75% on the medium of 1/2DKW ＋ 5.00 mg/L of IBA ＋ 1.00 mg/L of NAA; The suitable transplantation matrix was 3/4 sand ＋ 1/4 vermiculite,and survival rate of plant with root was above 79%. [Conclusion]The pistachio （Kerman） tissue culture technology system was established.展开更多
A multidisciplinary approach-anatomy, histochemistry and phytochemistry-was used to investigate the leaf structure, the content and the storage location of barbaloin in the leaves of Aloe vera L. var. chinensis (Haw.)...A multidisciplinary approach-anatomy, histochemistry and phytochemistry-was used to investigate the leaf structure, the content and the storage location of barbaloin in the leaves of Aloe vera L. var. chinensis (Haw.) Berg. Xeromorphic characteristics including secondary thickened epidermal cell walls, thicker cuticle, ambiguous differentiation of spongy and palisade tissues in the chlorenchyma, and well-developed aquiferous tissue could be seen in the leaves. Several large parenchymatous cells were observed at the phloem pole of the first ring of vascular bundles. The secondary ring of vascular bundles in the leaf base and the stomata, which are surrounded by five cells, have some classification significance in this species. The density of vascular bundles, the content of barbaloin and the intensity of histochemical reaction differed among leaf numbers Ll (annual leaf), L2 (biennial leaf), L3 (triennial leaf) and L4 (quadrennial leaf), and in different parts of the leaf. These three factors were highest in the youngest leaf, Ll, and top parts of all the leaves and lowest in the basal parts and the oldest leaf, L4. The density of vascular bundles had a positive correlation to the content of barbaloin. The histochemical results revealed that the small sheath cells that surrounded the bundles might be the location of barbaloin synthesis and the large parenchymatous cells beneath the sheath might be the storage places of this metabolite.展开更多
[Objective] The paper was to study the occurrence rule of Pistacia vera leaf brown spot and the biological characteristics of its pathogen.[Method] The occurrence rule of P.vera leaf brown spot was investigated in fie...[Objective] The paper was to study the occurrence rule of Pistacia vera leaf brown spot and the biological characteristics of its pathogen.[Method] The occurrence rule of P.vera leaf brown spot was investigated in field.A fungus was isolated and purified from the diseased tissue of P.vera,its cultural trait and morphological characteristics were observed in indoor experiment,and the classification status was identified according to its morphology.The effects of different temperatures on mycelial growth of the pathogen,as well as the effects of different temperatures and humidity on spore germination of the pathogen were also studied.[Result] P.vera leaf brown spot was a fungal disease,which mainly infected the leaves and fruits of P.vera,causing leaf spot and blight.The disease had serious incidence in introduction place of P.vera in China.The fungal disease was identified to be Alternaria alternaria,the appropriate temperature for mycelial growth was 20-25 ℃,the appropriate temperature for spore germination was 25-30 ℃,and the appropriate relative humidity was over 95%.[Conclusion] Late spring and early summer,as well as late summer and early autumn are two peak periods for incidence of the disease,continuous rain in summer is the decisive condition for the infection and outbreak of P.vera leaf brown spot.展开更多
A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents o...A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents of barbaloin and their environments in which they were growing was decided. A Hypersil ODS column (4.6 mm×200 mm, 5 μm)was used with a mobile phase of methanol-water (40:60, containing 0.1% acetic acid), the flow rate being 1.0 mL·min -1, detection wavelength at 359 nm, and the column temperature being 30℃. The linear range of barbaloin was between 0.0726 and 0.726 μg with a correlation coefficient of 0.9998 and the regression equation being Y=1.9202×10 6X-1801.9. Barbaloin was stable in methanol in 48 h and the instrument precision was 1.2% while the method precision was 4.9%. The contents of barbaloin of 12 samples ranged from 6.160 to 319.1 μg·g -1. The method developed was fast and simple with good reproducibility. There was high correlation between the contents of barbaloin and their growing environments.展开更多
利用自主开发的确定论数值反应堆物理计算程序NECP-X对VERA(virtual environment for reactor application)9#基准题进行全堆芯精细建模计算,显式描述了堆芯格架、管座、端塞、压紧弹簧及气腔等结构,计算得到了整个寿期中的临界硼质量分...利用自主开发的确定论数值反应堆物理计算程序NECP-X对VERA(virtual environment for reactor application)9#基准题进行全堆芯精细建模计算,显式描述了堆芯格架、管座、端塞、压紧弹簧及气腔等结构,计算得到了整个寿期中的临界硼质量分数,给出了3维全堆芯棒功率分布、燃料有效温度分布及慢化剂温度分布,并将计算结果与实测值进行了比较。结果表明,整个寿期中临界硼质量分数的计算值与实测值的偏差小于2.5×10^(-5),计算精度较好;在整个寿期内,随着燃耗的加深,堆芯功率分布逐渐展平,燃料有效温度及慢化剂温度的分布逐渐变得均匀,符合物理规律及预期。展开更多
AIM： To compare the effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing. METHODS： Male Spraque-Dawley rats （n=48） were divided into four groups. Group...AIM： To compare the effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing. METHODS： Male Spraque-Dawley rats （n=48） were divided into four groups. Group 1 served as control group, group 2 as gastric ulcer group without treatment, groups 3 and 4 as gastric ulcer treatment groups with sucralfate and Aloe vera. The rats from each group were divided into 2 subgroups for study of leukocyte adherence, TNF-α and IL-10 levels and gastric ulcer healing on days 1 and 8 after induction of gastric ulcer by 20% acetic acid. RESULTS： On day 1 after induction of gastric ulcer, the leukocyte adherence in postcapillary venule was significantly （P〈 0.05） increased in the ulcer groups when compared to the control group. The level of TNF-α was elevated and the level of IL-10 was reduced. In the ulcer groups treated with sucralfate and Aloe vera, leukocyte adherence was reduced in postcapillary venule. The level of IL-10 was elevated, but the level of TNF-a had no significant difference. On day 8, the leukocyte adherence in postcapillary venule and the level of TNF-α were still increased and the level of IL-10 was reduced in the ulcer group without treatment. The ulcer treated with sucralfate and Aloe vera had lower leukocyte adherence in postcapillary venule and TNF-α level. The level of IL-10 was still elevated compared to the ulcer group without treatment. Furthermore, histopathological examination of stomach on days 1 and 8 after induction of gastric ulcer showed that gastric tissue was damaged with inflammation. In the ulcer groups treated with sucralfate and Aloe vera on days 1 and 8, gastric inflammation was reduced, epithelial cell proliferation was enhanced and gastric glands became elongated. The ulcer sizes were also reduced compared to the ulcer group without treatment. CONCLUSION： Administration of 20% acetic acid can induce gastric inflammation, increase leukocyte adherence in postcapillary venule and TNF-α level and reduce IL-10 level. Aloe vera treatment can reduce leukocyte adherence and TNF-αlevel, elevate IL-10 level and promote gastric ulcer healing.展开更多
OBJECTIVE: To investigate the use of Aloe vera(A.vera) for the treatment of gastroesophageal reflux disease(GERD) symptoms and compare its effects with those of omeprazole and ranitidine.METHODS: In this pilot, random...OBJECTIVE: To investigate the use of Aloe vera(A.vera) for the treatment of gastroesophageal reflux disease(GERD) symptoms and compare its effects with those of omeprazole and ranitidine.METHODS: In this pilot, randomized controlled trial, 79 subjects were allocated to A. vera syrup(standardized to 5.0 mg polysaccharide per m L of syrup)at a dose of 10 m L/d, omeprazole capsule(20 g/d)or ranitidine tablet(150 mg in a fasted state in the morning and 150 mg 30 min before sleep at night)for a period of 4 weeks. The frequencies of eight main symptoms of GERD(heartburn, food regurgitation, flatulence, belching, dysphagia, nausea,vomiting and acid regurgitation) were assessed at weeks 2 and 4 of the trial.RESULTS: A. vera was safe and well tolerated and reduced the frequencies of all the assessed GERD symptoms, with no adverse events requiring withdrawal.CONCLUSION: A. vera may provide a safe and effective treatment for reducing the symptoms of GERD.展开更多
文摘BACKGROUND Polycythemia vera(PV)is a myeloproliferative neoplasm characterized by excessive blood cell production,which increases the risk of thrombosis.Ropeginterferon alfa-2b(RI)offers potential advantages over standard therapy(ST;including phlebotomy,hydroxyurea,and aspirin)by achieving hematologic and molecular responses.However,its comparative efficacy and safety remain understudied.We hypothesized that RI would improve hematologic and molecular outcomes but may differ in safety profiles compared to ST.AIM To evaluate the efficacy and safety of RI vs ST in patients with PV,focusing on hematologic response,molecular response,adverse events(AEs),and thrombotic risk.METHODS This Preferred Reporting Items for Systematic Reviews and Meta-Analyses-compliant meta-analysis included randomized controlled trials comparing RI to ST in adult PV patients.PubMed,EMBASE,ClinicalTrials.gov,and ScienceDirect were searched from inception to July 2025.Outcomes included complete hematological response(CHR),molecular response,AEs leading to discontinuation,JAK2V617F allele burden,thrombotic events,and phlebotomy frequency.Pooled odds ratios(ORs)and MD with 95%confidence intervals(95%CIs)were calculated using random-effects models.Risk of bias was assessed with Cochrane RoB 2;evidence certainty was evaluated via GRADE.RESULTS Five studies involving 477 RI and 456 ST patients were included.RI significantly improved CHR(OR=2.14,95%CI:1.18-3.88,P=0.002)and molecular response(OR=4.37,95%CI:0.99-19.38,P=0.05),with substantial heterogeneity(I²=76%and I²=93%,respectively).AEs leading to discontinuation were higher with RI(OR=3.89,95%CI:1.90-7.97,P=0.0002;I²=0%).No significant differences were observed in JAK2V617F allele burden(MD=-7.46,95%CI:-21.12 to 6.20,P=0.28;I²=90%)or thrombotic events(OR=0.93,95%CI:0.45-1.90,P=0.83;I²=0%).RI reduced phlebotomy frequency(MD=-1.52,95%CI:-2.37 to-0.67,P=0.0005;I²=0%).Most studies had low to moderate risk of bias;evidence certainty was moderate for CHR and AEs,low for molecular response and thrombotic events,and very low for allele burden.CONCLUSION RI offers superior hematologic and molecular responses compared to ST in PV but is associated with higher discontinuation rates due to AEs.Comparable thrombotic risk and reduced phlebotomy needs highlight its potential,though tolerability requires careful management.The high heterogeneity in certain outcomes and potential for publication bias warrant cautious interpretation of these findings.Further long-term studies are needed to optimize dosing and patient selection.
基金supported by grant from the Project of the National Natural Science Foundation of China(Grant No.82060028)funded by the Yunnan Blood Disease Clinical Medical Center(Grant No.2023YJZX-XY05)+2 种基金the Yunnan Health Commission(L2019003)to TSthe Yunnan Provincial Key Laboratory of Clinical Virology(Grant No.202205AG070005)the Yunnan Provincial Key Laboratory of Innovative Application of Traditional Chinese Medicine.
文摘Thrombosis is a leading cause of mortality and morbidity in patients suffering from polycythemia vera(PV).Drug pair Salviae Miltiorrhizae Radix et Rhizoma(Danshen,DS)and Notoginseng Radix et Rhizoma(Sanqi,SQ)is common traditional Chinese medicine(TCM)used in clinical practice to promote blood circulation and eliminate blood stasis.In this study,network pharmacology and molecular docking were used to analyze the potentially active ingredients and underlying mechanisms of drug pair DS-SQ against thrombosis after PV.These results show that 54 targets are related to both disease and the drug pair.Nineteen core targets,including IL-6 and AKT1,were screened.Luteolin and tanshinone IIa from DS as well as quercetin from SQ might be the major substances in the treatment of thrombosis after PV.KEGG enrichment analysis demonstrated that the lipid and atherosclerosis signaling pathway might play a significant role.These results provide valuable insights and a reference for the use of drug pair DS-SQ in management of thrombosis after PV and lay a foundation for further exploration of pharmacological effects.
基金supported by the Consejo Nacional de Ciencia y Tecnología(CONACyT)and Tecnológico de Monterreyreceived by CONACYT in the form of a Graduate Studies Scholarship。
文摘Corneal diseases,the second leading cause of global vision loss affecting over 10.5 million people,underscores the unmet demand for corneal tissue replacements.Given the scarcity of fresh donor corneas and the associated risks of immune rejection,corneal tissue engineering becomes imperative.Developing nanofibrous scaffolds that mimic the natural corneal structure is crucial for creating transparent and mechanically robust corneal equivalents in tissue engineering.Herein,Aloe Vera Extract(AVE)/Polycaprolactone(PCL)nanofibrous scaffolds were primed using electrospinning.The electrospun AVE/PCL fibers exhibit a smooth,bead-free morphology with a mean diameter of approximately 340±95 nm and appropriate light transparency.Mechanical measurements reveal Young’s modulus and ultimate tensile strength values of around 3.34 MPa and 4.58 MPa,respectively,within the range of stromal tissue.In addition,cell viability of AVE/PCL fibers was measured against Human Stromal Keratocyte Cells(HSKCs),and improved cell viability was observed.The cell-fiber interactions were investigated using scanning electron microscopy.In conclusion,the incorporation of Aloe Vera Extract enhances the mechanical,optical,hydrophilic,and biological properties of PCL fibers,positioning PCL/AVE fiber scaffolds as promising candidates for corneal stromal regeneration.
基金Supported by Jilin Province Health Research Talent Special Project,No.2020SCZ27.
文摘BACKGROUND The combination of polycythemia vera(PV)with pathological cardiac hypertro-phy is uncommon.In this study,we describe a case of PV accompanied by pa-thological cardiac hypertrophy.It is hypothesized that the pronounced cardiac hypertrophy in this patient has a strong connection with PV.CASE SUMMARY In 2021,a 34-year-old Chinese man experienced chest constriction,shortness of breath,and palpitations during vigorous activity.Each episode lasted several minutes and resolved spontaneously following cessation of vigorous activity.He occasionally experienced syncope and vertigo without a headache.He underwent cardiac magnetic resonance imaging and was diagnosed with“hypertrophic cardiomyopathy(HCM)”.He was discharged after receiving symptomatic treat-ment,which resulted in an improvement.He presented to our department with chest constriction,shortness of breath,and respiratory distress for one month while climbing to the second floor in 2023.His blood pressure was 180/100 mmHg at the time of admittance,and he was receiving antihypertensive treat-ment.He had a history of PV for 2 years without treatment.Symptomatic treat-ment was implemented concurrently with the administration of hydroxyurea upon admission.Good blood pressure control was observed during the long-term follow-up,and echocardiography did not reveal any progression of myocardial hypertrophy.CONCLUSION Clinicians managing PV patients should remain highly vigilant regarding the risks of thrombosis and cardio-vascular complications,particularly in those with refractory hypertension.
文摘Objective: This study compares hemoglobin and erythropoietin levels in patients with polycythemia vera and secondary polycythemia. Study Design: A retrospective cross-sectional study evaluating the serum erythropoietin and hemoglobin levels in patients with polycythemia vera and secondary polycythemia. This study was performed simultaneously in Texas state of the U.S. and Fars Province in Iran. Methods: Hemoglobin, hematocrit and erythropoietin test results were collected from patients aged 19 to 75 years who were diagnosed with polycythemia vera and secondary polycythemia. Patients records with history of thrombocythemia, congestive heart failure, dyspnea, anemia and pregnant woman were excluded from study. Patients in each decade of life were examined in separate groups, so that changes in hemoglobin related to aging did not affect the research results. Results: 75% of the patients were men, and 25% were women. A total of 1580 patients were analyzed in this study. 57.3% of patients in UTMB and 38.8 patients in Iran have hemoglobin level above 17 mg/dl. 74% of patients in UTMB and 88% of patients in Iran have erythropoietin below 10 IU/mL. Polycythemia in UTMB was more common in people over 50 and in Iran in patients under 50 years old. The serum hemoglobin and erythropoietin levels in patients with polycythemia vera were not significantly different in compare to secondary polycythemia patients. Data showed that there were 84 polycythemia patients per 100,000 people. The results of this study in UTMB and Iran showed that 4.5% and 7%, respectively, of patients with polycythemia had a positive JAK2 test. Conclusion: Low erythropoietin levels may not be helpful in differentiating polycythemia vera from secondary polycythemia. .
文摘Polycythemia vera manifests as a myeloproliferative neoplasm associated with diverse symptoms, including aquagenic pruritis. This systematic review addresses the pressing need to enhance the understanding of the disease’s symptomatology and optimize treatment strategies for improved patient outcomes. The rarity and low prevalence of polycythemia vera underscore the importance of this investigation, as existing standard of care involves a multifaceted approach and significant healthcare costs. Despite advancement in therapeutic options, persistent symptoms and resistance to first-line treatments pose challenges. Ruxolitinib has emerged as a promising intervention, demonstrating clinically significant improvement for patients. This systematic review appraises three randomized controlled trials, shedding light on the efficacy of ruxolitinib and its potential to ameliorate pruritis symptoms in symptomatic patients.
文摘The International Union of Geological Sciences(IUGS)is celebrating women and girls in geoscience and in celebrating the 60^(th)anniversary of the IUGS,we must applaud those women who have pioneered the work of the International Union of Geosciences.Between 1976 to 1980,Prof.Rudolf Trümpy(Switzerland)was President of the IUGS and William Watt Hutchison(Canada),its Secretary General and later President(1984–1987).Hutchison appointed Canadian Vera Lafferty to his Secretariat under the auspices of the Geological Survey of Canada in Ottawa.In this post,she created not only the flagship Episodes magazine but forged a new style across the IUGS platform.Vera Lafferty’s major contributions to the foundation and style of Episodes and the promotion of the IUGS are recognized.
基金Supported by Beijing Natural Science Foundation (6072005)Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality(PXM2009-014207-076874)~~
文摘[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ＇Kerman＇,tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result]Mediums of 1/2DKW and 1/2DKW＋3.00 mg/L of 6-BA ＋0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio; Proliferation coefficient was 3.6 on the medium of 1/2DKW ＋4.00 mg/L of 6-BA ＋ 0.05 mg/L of IBA; Rooting rate was up to 75% on the medium of 1/2DKW ＋ 5.00 mg/L of IBA ＋ 1.00 mg/L of NAA; The suitable transplantation matrix was 3/4 sand ＋ 1/4 vermiculite,and survival rate of plant with root was above 79%. [Conclusion]The pistachio （Kerman） tissue culture technology system was established.
文摘A multidisciplinary approach-anatomy, histochemistry and phytochemistry-was used to investigate the leaf structure, the content and the storage location of barbaloin in the leaves of Aloe vera L. var. chinensis (Haw.) Berg. Xeromorphic characteristics including secondary thickened epidermal cell walls, thicker cuticle, ambiguous differentiation of spongy and palisade tissues in the chlorenchyma, and well-developed aquiferous tissue could be seen in the leaves. Several large parenchymatous cells were observed at the phloem pole of the first ring of vascular bundles. The secondary ring of vascular bundles in the leaf base and the stomata, which are surrounded by five cells, have some classification significance in this species. The density of vascular bundles, the content of barbaloin and the intensity of histochemical reaction differed among leaf numbers Ll (annual leaf), L2 (biennial leaf), L3 (triennial leaf) and L4 (quadrennial leaf), and in different parts of the leaf. These three factors were highest in the youngest leaf, Ll, and top parts of all the leaves and lowest in the basal parts and the oldest leaf, L4. The density of vascular bundles had a positive correlation to the content of barbaloin. The histochemical results revealed that the small sheath cells that surrounded the bundles might be the location of barbaloin synthesis and the large parenchymatous cells beneath the sheath might be the storage places of this metabolite.
基金Supported by "948" Projects of State Forestry Administration "Introduction of Fine Varieties and Cultivation and Management Techniques of Pistacia vera" (96-4-71-02)~~
文摘[Objective] The paper was to study the occurrence rule of Pistacia vera leaf brown spot and the biological characteristics of its pathogen.[Method] The occurrence rule of P.vera leaf brown spot was investigated in field.A fungus was isolated and purified from the diseased tissue of P.vera,its cultural trait and morphological characteristics were observed in indoor experiment,and the classification status was identified according to its morphology.The effects of different temperatures on mycelial growth of the pathogen,as well as the effects of different temperatures and humidity on spore germination of the pathogen were also studied.[Result] P.vera leaf brown spot was a fungal disease,which mainly infected the leaves and fruits of P.vera,causing leaf spot and blight.The disease had serious incidence in introduction place of P.vera in China.The fungal disease was identified to be Alternaria alternaria,the appropriate temperature for mycelial growth was 20-25 ℃,the appropriate temperature for spore germination was 25-30 ℃,and the appropriate relative humidity was over 95%.[Conclusion] Late spring and early summer,as well as late summer and early autumn are two peak periods for incidence of the disease,continuous rain in summer is the decisive condition for the infection and outbreak of P.vera leaf brown spot.
文摘A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents of barbaloin and their environments in which they were growing was decided. A Hypersil ODS column (4.6 mm×200 mm, 5 μm)was used with a mobile phase of methanol-water (40:60, containing 0.1% acetic acid), the flow rate being 1.0 mL·min -1, detection wavelength at 359 nm, and the column temperature being 30℃. The linear range of barbaloin was between 0.0726 and 0.726 μg with a correlation coefficient of 0.9998 and the regression equation being Y=1.9202×10 6X-1801.9. Barbaloin was stable in methanol in 48 h and the instrument precision was 1.2% while the method precision was 4.9%. The contents of barbaloin of 12 samples ranged from 6.160 to 319.1 μg·g -1. The method developed was fast and simple with good reproducibility. There was high correlation between the contents of barbaloin and their growing environments.
文摘利用自主开发的确定论数值反应堆物理计算程序NECP-X对VERA(virtual environment for reactor application)9#基准题进行全堆芯精细建模计算,显式描述了堆芯格架、管座、端塞、压紧弹簧及气腔等结构,计算得到了整个寿期中的临界硼质量分数,给出了3维全堆芯棒功率分布、燃料有效温度分布及慢化剂温度分布,并将计算结果与实测值进行了比较。结果表明,整个寿期中临界硼质量分数的计算值与实测值的偏差小于2.5×10^(-5),计算精度较好;在整个寿期内,随着燃耗的加深,堆芯功率分布逐渐展平,燃料有效温度及慢化剂温度的分布逐渐变得均匀,符合物理规律及预期。
基金Supported by Rajadapiseksompoj Research Fund, Faculty of Medicine and Research Fund by Graduate School, Chulalongkom University
文摘AIM： To compare the effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing. METHODS： Male Spraque-Dawley rats （n=48） were divided into four groups. Group 1 served as control group, group 2 as gastric ulcer group without treatment, groups 3 and 4 as gastric ulcer treatment groups with sucralfate and Aloe vera. The rats from each group were divided into 2 subgroups for study of leukocyte adherence, TNF-α and IL-10 levels and gastric ulcer healing on days 1 and 8 after induction of gastric ulcer by 20% acetic acid. RESULTS： On day 1 after induction of gastric ulcer, the leukocyte adherence in postcapillary venule was significantly （P〈 0.05） increased in the ulcer groups when compared to the control group. The level of TNF-α was elevated and the level of IL-10 was reduced. In the ulcer groups treated with sucralfate and Aloe vera, leukocyte adherence was reduced in postcapillary venule. The level of IL-10 was elevated, but the level of TNF-a had no significant difference. On day 8, the leukocyte adherence in postcapillary venule and the level of TNF-α were still increased and the level of IL-10 was reduced in the ulcer group without treatment. The ulcer treated with sucralfate and Aloe vera had lower leukocyte adherence in postcapillary venule and TNF-α level. The level of IL-10 was still elevated compared to the ulcer group without treatment. Furthermore, histopathological examination of stomach on days 1 and 8 after induction of gastric ulcer showed that gastric tissue was damaged with inflammation. In the ulcer groups treated with sucralfate and Aloe vera on days 1 and 8, gastric inflammation was reduced, epithelial cell proliferation was enhanced and gastric glands became elongated. The ulcer sizes were also reduced compared to the ulcer group without treatment. CONCLUSION： Administration of 20% acetic acid can induce gastric inflammation, increase leukocyte adherence in postcapillary venule and TNF-α level and reduce IL-10 level. Aloe vera treatment can reduce leukocyte adherence and TNF-αlevel, elevate IL-10 level and promote gastric ulcer healing.
基金Supported by the Clinical Trial Research CenterTehranIran
文摘OBJECTIVE: To investigate the use of Aloe vera(A.vera) for the treatment of gastroesophageal reflux disease(GERD) symptoms and compare its effects with those of omeprazole and ranitidine.METHODS: In this pilot, randomized controlled trial, 79 subjects were allocated to A. vera syrup(standardized to 5.0 mg polysaccharide per m L of syrup)at a dose of 10 m L/d, omeprazole capsule(20 g/d)or ranitidine tablet(150 mg in a fasted state in the morning and 150 mg 30 min before sleep at night)for a period of 4 weeks. The frequencies of eight main symptoms of GERD(heartburn, food regurgitation, flatulence, belching, dysphagia, nausea,vomiting and acid regurgitation) were assessed at weeks 2 and 4 of the trial.RESULTS: A. vera was safe and well tolerated and reduced the frequencies of all the assessed GERD symptoms, with no adverse events requiring withdrawal.CONCLUSION: A. vera may provide a safe and effective treatment for reducing the symptoms of GERD.
