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Endoscopic trans-esophageal submucosal tunneling surgery:A new therapeutic approach for diseases located around the aorta ventralis 被引量:3
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作者 Ying Xiong Qian-Qian Chen +3 位作者 Ning-Li Chai Shun-Chang Jiao En-Qiang Ling Hu 《World Journal of Gastroenterology》 SCIE CAS 2019年第1期85-94,共10页
AIM To assess the efficiency of endoscopic trans-esophageal submucosal tunneling surgery(EESTS) technique for diseases located around the aorta ventralis.METHODS Nine pigs were assigned to EESTs. The procedures were a... AIM To assess the efficiency of endoscopic trans-esophageal submucosal tunneling surgery(EESTS) technique for diseases located around the aorta ventralis.METHODS Nine pigs were assigned to EESTs. The procedures were as follows: First, a long esophageal submucosal tunnel was established. Second, full-thickness myotomy was created. Third, an endoscope was entered into the abdominal cavity through a muscle incision and the endoscope was around the aorta ventralis. Eventually,celiac trunk ganglion neurolysis, partial hepatectomy and splenectomy, partial tissue resection in the area of the posterior peritoneum, and endoscopic submucosal dissection(ESD) combined with lymph node dissection were performed. The animals were given antibiotics for 5 d and necropsied 7 d after surgery.RESULTS In all surgeries, one pig died from intraperitoneal hemorrhage after doing partial splenectomy, while the other pigs were alive after successfully operating other surgeries. For surgery of celiac trunk ganglion damage, at necropsy, there was no exudation in the abdominal cavity. Regarding surgery of partial hepatectomy, the wound with part healing was observed in the left hepatic lobe, and no bleeding or obvious exudation was seen. In surgery of partial splenectomy, massive hemorrhage was observed on the splenic wound surface, and the metal clips could not stop bleeding. After surgery of retroperitoneal tissue resection, mild tissue adhesion was observed in the abdominal cavity of one animal, and another one suffered from severe infection. For surgery of ESD and lymph node dissection, a moderate tissue adhesion was observed.CONCLUSION EESTS is a feasible and safe technique for diseases located around the aorta ventralis. 展开更多
关键词 ENDOSCOPIC trans-esophageal SUBMUCOSAL TUNNELING SURGERY Diseases around the AORTA ventralis ENDOSCOPIC SUBMUCOSAL TUNNELING technique Abdominal SURGERY Animal model
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Unilateral rupture of the serratus ventralis muscle in a foal
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作者 Mohamed Mostafa Shokry 《Journal of Coastal Life Medicine》 2016年第4期335-336,共2页
A 3-year-old Arabian filly with a history of abnormal instability of the scapula of the left foreleg following an accident during training.Unilateral partial rupture of serratus ventralis was diagnosed after ultrasono... A 3-year-old Arabian filly with a history of abnormal instability of the scapula of the left foreleg following an accident during training.Unilateral partial rupture of serratus ventralis was diagnosed after ultrasonographic examination of the affected leg in comparison with the other sound leg. 展开更多
关键词 Muscle rupture Serratus ventralis SCAPULA ULTRASONOGRAPHY
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长鳍吻鮈性腺发育的初步观察 被引量:10
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作者 姚建伟 杨德国 +2 位作者 刘阳 吴兴兵 高宇鹏 《淡水渔业》 CSCD 北大核心 2016年第2期107-112,共6页
采用常规石蜡切片技术对野生长鳍吻鮈(Rhinogobio ventralis(Sauvage et Dabry))性腺进行了组织学观察,描述了其各类型生殖细胞形态及卵巢、精巢的分期特征。结果显示:长鳍吻鮈卵巢为细线状或扁条状,精巢为细线状或直棍状;卵细胞发生经... 采用常规石蜡切片技术对野生长鳍吻鮈(Rhinogobio ventralis(Sauvage et Dabry))性腺进行了组织学观察,描述了其各类型生殖细胞形态及卵巢、精巢的分期特征。结果显示:长鳍吻鮈卵巢为细线状或扁条状,精巢为细线状或直棍状;卵细胞发生经历了卵原细胞、初级卵母细胞和次级卵母细胞3个阶段,精细胞发生经历了精原细胞、初级精母细胞、次级精母细胞、精子细胞及精子5个阶段;性腺发育分为6个时期,卵巢中第Ⅱ时相晚期至Ⅲ时相早期的初级卵母细胞中出现核仁外排现象;同一时期卵巢中存在多种时相的卵母细胞,故推测其卵巢发育类型为部分同步型,精巢为小叶型结构。 展开更多
关键词 长鳍吻鮈(Rhinogobio ventralis(Sauvage ET Dabry)) 性腺发育 组织切片 卵巢 精巢
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基于循环水养殖系统的长鳍吻鮈亲鱼培育、催产和孵化技术初探 被引量:3
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作者 朱永久 吴兴兵 +1 位作者 何勇凤 杨德国 《淡水渔业》 CSCD 北大核心 2018年第3期101-106,共6页
2012—2015年,采用室内循环水养殖系统开展了长鳍吻鮈(Rhinogobio ventralis)亲鱼培育、人工催产和孵化技术研究。结果显示:3年间长鳍吻鮈均能在循环水养殖系统中培育成熟,2014年5月—2015年4月间培育存活率和成熟率最高,分别为85.3%~1... 2012—2015年,采用室内循环水养殖系统开展了长鳍吻鮈(Rhinogobio ventralis)亲鱼培育、人工催产和孵化技术研究。结果显示:3年间长鳍吻鮈均能在循环水养殖系统中培育成熟,2014年5月—2015年4月间培育存活率和成熟率最高,分别为85.3%~100%和77.3%~100%;对培育成熟的亲鱼进行了4种不同外源激素(PG、HCG、LRH-A2、LRH-A2+DOM)催产实验,结果为LRH-A26μg/kg+DOM 5 mg/kg合剂催产率最高,达88.3%;14、16、18、20和22℃5个温度组的受精卵孵化实验结果显示,孵化时间(H)与温度(T)呈显著负相关关系(H=0.573T2-30.393T+418.178,R2=0.982,P<0.01),18℃和20℃组的孵化率分别为54.27%和56.07%,显著高于其他各组,表明长鳍吻受精卵适宜孵化温度范围为18~20℃。 展开更多
关键词 长鳍吻鮈(Rhinogobio ventralis) 封闭循环水 亲鱼培育 人工催产 孵化
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长鳍吻免疫因子基因cDNA部分序列克隆及其组织表达差异
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作者 李学梅 吴兴兵 +2 位作者 王旭歌 朱永久 杨德国 《淡水渔业》 CSCD 北大核心 2018年第1期15-19,33,共6页
根据鲤科鱼类同源序列设计并合成了长鳍吻(Rhinogobio ventralis)应激因子HSP70、抗体IgM、抑炎性因子IL-10和促炎性因子IL-1b基因特异引物,以长鳍吻皮肤组织总RNA为模板,RT-PCR扩增获得长度分别为412、463、520和217 bp的上述4种免疫... 根据鲤科鱼类同源序列设计并合成了长鳍吻(Rhinogobio ventralis)应激因子HSP70、抗体IgM、抑炎性因子IL-10和促炎性因子IL-1b基因特异引物,以长鳍吻皮肤组织总RNA为模板,RT-PCR扩增获得长度分别为412、463、520和217 bp的上述4种免疫因子基因cDNA部分序列。同时通过RT-PCR比较患小瓜虫病长鳍吻和对照组长鳍吻皮肤和肠道组织中各免疫因子的表达差异,结果显示:在皮肤组织中,IgM和IL-10在感染组鱼体中表达量显著高于对照鱼(P<0.05),HSP 70和IL-1b的表达则没有差异;在肠道组织中,IgM、IL-10和IL-1b在感染组鱼体中表达量显著增高,HSP 70的表达量没有差异。本研究首次对长鳍吻的免疫因子进行了分析,指出免疫因子IgM和IL-10在鱼体中免疫应答反应中较为灵敏,为养殖过程中长鳍吻应激监测方面奠定了理论基础。 展开更多
关键词 免疫因子 克隆 组织差异 长鳍吻(Rhinogobio ventralis)
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