Natural hybridization is known to play a vital role in speciation;however,the mechanisms underlying the early stages of natural hybridization remain unclear.Where two plant species come into contact,two driving forces...Natural hybridization is known to play a vital role in speciation;however,the mechanisms underlying the early stages of natural hybridization remain unclear.Where two plant species come into contact,two driving forces may balance the dynamic consequences of hybridization:fusion by hybridization-mediated gene flow,and separation by reproductive isolation(RI)(Ma et al.,2010a,b;Chang et al.,2022).展开更多
Background The decline in reproductive performance of aged hens is mainly attributed to oxidative damage in reproductive organs,hepatic lipid metabolism disorders,and intestinal microbiota dysbiosis.Glycyrrhizin(GL)ha...Background The decline in reproductive performance of aged hens is mainly attributed to oxidative damage in reproductive organs,hepatic lipid metabolism disorders,and intestinal microbiota dysbiosis.Glycyrrhizin(GL)has been proven to enhance antioxidant capacity,regulate lipid metabolism and gut microbiota in mammals,but its efficacy in hens remains unclear.Hence,this study aimed to investigate whether dietary GL supplementation improves reproductive performance in hens during the late laying stage by modulating intestinal microbiota composition,hepatic lipid metabolism and ovarian antioxidant status.Results Dietary supplementation with 100 mg/kg GL significantly improved the egg production rate,egg quality,and hatching rate in aged breeder hens(P<0.05).GL supplementation also increased the serum levels of HDLC,TP and ALB,and enhanced the antioxidant capacity in both serum and ovary(P<0.05).In addition,dietary GL elevated the serum progesterone(P4)levels by enhancing the transcription level of steroid synthesis key enzymes(CYP11A1 and 3β-HSD)in the ovary(P<0.05).Dietary GL also promoted the synthesis and transport of vitellogenin(VTG)by upregulating the VTG-Ⅱ(P<0.05)and APOV1(P=0.077)expression levels in the liver,thereby increasing the number of grade follicles and small yellow follicles.Moreover,dietary GL enhanced hepatic fatty acidβ-oxidation by upregulating PPARαand CPT-I(P<0.05),and downregulating ACC expression levels(P<0.05).In agreement,liver metabolomics analysis revealed that dietary GL supplementation significantly altered hepatic metabolism,with 389 differentially identified metabolites(P<0.05).The key metabolites(e.g.,taurocholic acid,tauroursodeoxycholic acid,nicotinuric acid,glycodeoxycholic acid(hydrate))were identified,and they were mainly functionally enriched in betaalanine metabolism nicotinate,taurine and hypotaurine metabolism(P<0.05).Finally,16S rRNA gene sequencing revealed that dietary GL reversed age-induced changes in gut microbiota composition,characterized by a significant increase in Lactobacillus abundance and a decrease in Bacteroides(P<0.05).Conclusions These results collectively demonstrate that dietary supplementation with 100 mg/kg GL improved reproductive performance by reversing age-induced changes in gut microbiota,enhancing hepatic vitellogenin synthesis,and ameliorating ovarian function in aged breeder hens.This study suggests that dietary GL is a potential strategy to improve reproductive performance in broiler breeder hens during the late laying period.展开更多
Background:Epidemiological studies have confirmed that longer exposure to insecticides like cypermethrin(CYP)significantly increases the risk of male reproductive toxicity.Crocus sativus L.has been recognized due to i...Background:Epidemiological studies have confirmed that longer exposure to insecticides like cypermethrin(CYP)significantly increases the risk of male reproductive toxicity.Crocus sativus L.has been recognized due to its therapeutic properties,but its exact role and molecular mechanisms in treatment of reproductive dysfunction remain unclear.Methods:During this study,36 rats were randomly divided into six groups(n=6):control,CYP-induced(60 mg/kg),standard(leuprolide 3 mg/kg)and three treatment groups receiving aqueous,ethanolic,and oil extracts(50 mg/kg or 20 mL/kg)for post-toxicity induction.Results:The finding represented that exposure of CYP significantly increased oxidative stress,disrupted testicular architecture,and markedly reduced testosterone levels(P<0.05).Importantly,Crocus sativus L.treatment alleviated these changes by increasing the expression of Nrf2(nuclear factor erythroid 2-related factor 2),restoring the activity of antioxidant enzymes,and enhancing testicular histomorphology.Surprisingly,molecular docking established a high binding affinity of Crocus sativus L.phytoconstituents such as gallic acid,cinnamic acid and quercetin to the Nrf2-Keap1 complex.It is worth noting that,Crocus sativus L.exhibited a high level of protection against reproductive toxicity caused by CYP in male rats,which was mediated by the activation of Nrf2 pathway,reduction of oxidative damage,and favorable ADMET characteristics.Conclusion:Notably,this research provides a more valid,safe,and effective method of developing new drugs for reproductive disorders,however,further investigation is needed to support the research findings and implement it in clinical practice.展开更多
Elaeagnaceae Juss. belongs to the family Elaeagnus L. and Shepherdia Nutt. This study aims at the significance and vegetative reproduction of species of the family (Elaeagnus macrophylla Thunb. and Shepherdia canadens...Elaeagnaceae Juss. belongs to the family Elaeagnus L. and Shepherdia Nutt. This study aims at the significance and vegetative reproduction of species of the family (Elaeagnus macrophylla Thunb. and Shepherdia canadensis (L.) Nutt.) in the national economy. The trials were carried out in the experimental site of SamSU’s Botanic Garden, which is named after Sharof Rashidov. The study’s findings are based on the results obtained by planting cuttings of S. canadensis and E. macrophylla species of varying sizes and durations, as well as the cuttings’ roots and the impacts of growth chemicals.展开更多
Paris polyphylla Smith is an important medicinal plant that is considered vulnerable in many parts of the world. The species exhibits poor adaptability to changed environment and susceptible to climatic conditions for...Paris polyphylla Smith is an important medicinal plant that is considered vulnerable in many parts of the world. The species exhibits poor adaptability to changed environment and susceptible to climatic conditions for reproductive vigor. During the present study the species was studied in four different natural niches as well as synthetic habitats to check the adaptability to artificial conditions, its morphological and physiological characters and its interaction in its community so that conservation strategies could be worked out. The present study indicates that P. polyphylla exhibits healthy growth as well as reproduction in undisturbed area with canopy covers over 80%. The plant grows in well-drained rich humus soil and grows well with certain key associated species such as Quercus species, Taxus baccata, Aconitum species, Eupatorium adenophorum, Smilax species etc. Many plants were non-flowering and more than 60% were found non-flowering in its habitat during its flowering season in any particular year. There are clear morphological changes as the flowers stages from pre-fertilization to post-fertilization. Arrangement of anthers in two whorls shows a distinct and unique floral display. Light is an important factor during seed setting and less than 50% shade lowers the seed productivity very significantly. Predators such as slugs and snails are found on the plant. Urgent conservation and new innovative ways are necessary to propagate this economically important plant to ensure its continuity.展开更多
The WRKY transcription factors play important roles in plant growth and resistance,but only a few members have been identified in strawberry.Here we identified a WRKY transcription factor,FvWRKY50,in diploid strawberr...The WRKY transcription factors play important roles in plant growth and resistance,but only a few members have been identified in strawberry.Here we identified a WRKY transcription factor,FvWRKY50,in diploid strawberry which played essential roles in strawberry vegetative growth,and reproductive growth.Knocking out FvWRKY50 by genome editing accelerated flowering time and leaf senescence but delayed anthocyanin accumulation in fruit.Further analysis showed that FvWRKY50 acted as a transcriptional repressor to negatively regulate the expression of flowering-and leaf senescence-related genes,including FvFT2,FvCO,FvFT3,and FvSAUR36.Notably,FvWRKY50 directly upregulated the expression of FvCHI and FvDFR by binding their promoter under normal conditions,but at low temperature FvWRKY50 was phosphorylated by FvMAPK3 and then induced protein degradation by ubiquitination,delaying anthocyanin accumulation.In addition,the homozygous mutant of FvWRKY50 was smaller while the biallelic mutant showed normal size.These new findings provide important clues for us to further reveal the regulatory mechanisms of strawberry growth and fruit ripening.展开更多
The efficacy of crude extracts of Senna alata in the improvement of vegetative and reproductive growth in Celosia aregentea was investigated. Fresh leaves of S. alata were blended with a homogenizer in 1 litre of dist...The efficacy of crude extracts of Senna alata in the improvement of vegetative and reproductive growth in Celosia aregentea was investigated. Fresh leaves of S. alata were blended with a homogenizer in 1 litre of distilled water. The resultant green paste was filtered under suction. Different concentrations (75%, 50%, 40%, 30%, 25%, 12%, 10%, and 5%) were prepared from the 100% crude extract. Seeds of C. argentea were presoaked in these different concentrations including a control (0%) and planted out after 24 hours. Results obtained showed that seedling height, leaf area, dry weight and leaf area ratio were promoted and enhanced by presoaking seeds in the extract. At the end of the experimental period (six weeks), seedling height in 75% and 100% treatments were 109 ± 16.12 cm and 117 ± 19.32 cm, leaf area 128 ± 17.91 cm2 and 125 ± 18.12 cm2, dry weight 7.48 kg and 7.0 kg respectively. Seedlings raised from seeds presoaked in water (control) however, flowered earlier (8 weeks) than the treatments (10 weeks in 75% and 100%). Presoaking seeds of C. argentea in crude extracts of S. alata before planting is recommended for optimum production of the leafy vegetable. The procedure is cheap and easily implementable by resource-poor farmers who are the main growers of C. argentea.展开更多
Compounds isolated from Epimedium include the total flavonoids of Epimedium,icariin,and its metabolites(icaritin,icariside Ⅰ,and icariside Ⅱ),which have similar molecular structures.Modern pharmacological research a...Compounds isolated from Epimedium include the total flavonoids of Epimedium,icariin,and its metabolites(icaritin,icariside Ⅰ,and icariside Ⅱ),which have similar molecular structures.Modern pharmacological research and clinical practice have proved that Epimedium and its active components have a wide range of pharmacological effects,especially in improving sexual function,hormone regulation,anti-osteoporosis,immune function regulation,anti-oxidation,and anti-tumor activity.To date,we still need a comprehensive source of knowledge about the pharmacological effects of Epimedium and its bioactive compounds on the male reproductive system.However,their actions in other tissues have been reviewed in recent years.This review critically focuses on the Epimedium,its bioactive compounds,and the biochemical and molecular mechanisms that modulate vital pathways associated with the male reproductive system.Such intrinsic knowledge will significantly further studies on the Epimedium and its bioactive compounds that protect the male reproductive system and provide some guidances for clinical treatment of related male reproductive disorders.展开更多
BACKGROUND Breast cancer is one of the most prevalent causes of morbidity and mortality worldwide,presenting an increasing public health challenge,particularly in lowincome and middle-income countries.However,data on ...BACKGROUND Breast cancer is one of the most prevalent causes of morbidity and mortality worldwide,presenting an increasing public health challenge,particularly in lowincome and middle-income countries.However,data on the knowledge,attitudes,and preventive practices regarding breast cancer and the associated factors among females in Wollo,Ethiopia,remain limited.AIM To assess the impact of family history(FH)of breast disease on knowledge,attitudes,and breast cancer preventive practices among reproductive-age females.METHODS A community-based cross-sectional study was conducted in May and June 2022 in Northeast Ethiopia and involved 143 reproductive-age females with FH of breast diseases and 209 without such a history.We selected participants using the systematic random sampling technique.We analyzed the data using Statistical Package for Social Science version 25 software,and logistic regression analysis was employed to determine odds ratios for variable associations,with statistical significance set at P<0.05.RESULTS Among participants with FH of breast diseases,the levels of knowledge,attitudes,and preventive practices were found to be 83.9%[95%confidence interval(CI):77.9-89.9],49.0%(95%CI:40.8-57.1),and 74.1%(95%CI:66.9-81.3),respectively.In contrast,among those without FH of breast diseases,these levels were significantly decreased to 10.5%(95%CI:6.4-14.7),32.1%(95%CI:25.7-38.4),and 16.7%(95%CI:11.7-21.8),respectively.This study also indicated that knowledge,attitudes,and preventive practices related to breast cancer are significantly higher among participants with FH of breast diseases compared to those without HF breast diseases.CONCLUSION Educational status,monthly income,and community health insurance were identified as significant factors associated with the levels of knowledge,attitudes,and preventive practices regarding breast cancer among reproductive-age females.展开更多
Background Sow longevity and reproductivity are essential in the modern swine industry.Although many studies have focused on the genetic and genomic factors for selection,little is known about the associations between...Background Sow longevity and reproductivity are essential in the modern swine industry.Although many studies have focused on the genetic and genomic factors for selection,little is known about the associations between the microbiome and sows with longevity in reproduction.Results In this study,we collected and sequenced rectal and vaginal swabs from 48 sows,nine of which completed up to four parities(U4P group),exhibiting reproductive longevity.We first identified predictors of sow longevity in the rectum(e.g.,Akkermansia)and vagina(e.g.,Lactobacillus)of the U4P group using RandomForest in the early breeding stage of the first parity.Interestingly,these bacteria in the U4P group showed decreased predicted KEGG gene abundance involved in the biosynthesis of amino acids.Then,we tracked the longitudinal changes of the micro-biome over four parities in the U4P sows.LEfSe analysis revealed parity-associated bacteria that existed in both the rectum and vagina(e.g.,Streptococcus in Parity 1,Lactobacillus in Parity 2,Veillonella in Parity 4).We also identi-fied patterns of bacterial change between the early breeding stage(d 0)and d 110,such as Streptococcus,which was decreased in all four parties.Furthermore,sows in the U4P group with longevity potential also showed better reproductive performance.Finally,we discovered bacterial predictors(e.g.,Prevotellaceae NK3B31 group)for the total number of piglets born throughout the four parities in both the rectum and vagina.Conclusions This study highlights how the rectal and vaginal microbiome in sows with longevity in reproduc-tion changes within four parities.The identification of parity-associated,pregnancy-related,and reproductive performance-correlated bacteria provides the foundation for targeted microbiome modulation to improve animal production.展开更多
Stromal vascular fraction(SVF)is a complex mixture derived from adipose tissue,consisting of a variety of cells.Due to its potential for tissue repair,immunomod-ulation,and support of angiogenesis,SVF represents a pro...Stromal vascular fraction(SVF)is a complex mixture derived from adipose tissue,consisting of a variety of cells.Due to its potential for tissue repair,immunomod-ulation,and support of angiogenesis,SVF represents a promising frontier in regenerative medicine and offers potential therapy for a range of disease condi-tions.In this article,we delve into the mechanisms through which SVF exerts its effects and explore its potential applications in treating both male and female reproductive disorders,including erectile dysfunction,testicular injury,stress urinary incontinence and intrauterine adhesion.展开更多
Background Hexafluoropropylene oxide dimer acid(GenX),a substitute for per-and polyfluoroalkyl substances,has been widely detected in various environmental matrices and foods recently,attracting great attention.Howeve...Background Hexafluoropropylene oxide dimer acid(GenX),a substitute for per-and polyfluoroalkyl substances,has been widely detected in various environmental matrices and foods recently,attracting great attention.However,a systematic characterization of its reproductive toxicity is still missing.This study aims to explore the male reproductive toxicity caused by GenX exposure and the potential cellular and molecular regulatory mechanisms behind it.Results Normally developing mice were exposed to GenX,and testicular tissue was subsequently analyzed and validated using single-cell RNA sequencing.Our results revealed that GenX induced severe testicular damage,disrupted the balance between undifferentiated and differentiated spermatogonial stem cells,and led to strong variation in the cellular dynamics of spermatogenesis.Furthermore,GenX exposure caused global upregulation of testicular somatic cellular inflammatory responses,increased abnormal macrophage differentiation,and attenuated fibroblast adhesion,disorganizing the somatic-germline interactions.Conclusions In conclusion,this study revealed complex cellular dynamics and transcriptome changes in mouse testis after GenX exposure,providing a valuable resource for understanding its reproductive toxicity.展开更多
Variation in the vocal behavior of nonhuman vertebrates includes graded transitions and more dramatic changes.Wapiti males produce a reproductive bugle that has a fundamental frequency that surpasses 2,000 Hz with evi...Variation in the vocal behavior of nonhuman vertebrates includes graded transitions and more dramatic changes.Wapiti males produce a reproductive bugle that has a fundamental frequency that surpasses 2,000 Hz with evidence of biphonation and other nonlinear phenomena.Here,we analyze the acoustic structure of captive wapiti vocalizations to compare the male bugle with 3 categories of distress vocalizations:neonate distress(capture)calls,calf isolation calls,and adult female isolation calls.These 4 high-arousal call categories serve a common general function in recruiting conspecifics but occur in different behavioral contexts(capture,isolation,reproduction).Our goal was to distinguish characteristics that vary in graded steps that may correspond to an animal’s age or size from characteristics that are unique to the bugle.Characteristics of the high and loud fundamental(G0)varied in an age/size-graded manner with a decrease in minimum G0,an increase in the maximum and range of G0,with no evidence of sex differences.The nonlinear phenomena of deterministic chaos,biphonation,and frequency jumps were present in all 4 call categories and became more common from the distress vocalizations of neonates to calves to adult females to the male bugle.Two temporal characteristics sharply distinguished the bugle from the 3 categories of distress vocalizations:these included a prolonged call duration and a maximum G0 that occurred much later in the call for the bugle than for distress vocalizations.Our results suggest that distress vocalizations of different age groups and the reproductive bugle of wapiti share a high G0,with age/size-graded changes in G0 and nonlinear phenomena,but differ sharply in temporal characteristics.展开更多
Background: Diabetes is a set of metabolic abnormalities typified by hyperglycemia believed to be associated with aberrant insulin secretion, insulin action, or both. Erectile dysfunction and infertility are globally ...Background: Diabetes is a set of metabolic abnormalities typified by hyperglycemia believed to be associated with aberrant insulin secretion, insulin action, or both. Erectile dysfunction and infertility are globally prevalent complications among male diabetic patients. There is a need to understand the role of a male reproductive hormone in the etiology of infertility in diabetic patients. Objective: This study aims to determine the effect of diabetes mellitus on the Male reproductive hormone (testosterone) on diabetic patients attending the Urology unit of Federal University Teaching Hospital, Abakaliki, Ebonyi State, Nigeria and the effect of industrial sugar consumption in rats model. Materials and Methods: 30 samples (including repeats, n = 3) were collected from known diabetic patients and 30 (including repeats, n = 3) control-non diabetic healthy individuals. Five millilitres (5 ml) of blood was collected from the antecubital vein of each participant and placed into a plain container for serum testosterone measurement using the enzyme-linked immunoassay technique. Blood was also collected from the same subjects in a fluoride oxalate container for plasma enzymatic glucose estimation using a Randox Glucose oxidase kit. For in vivo rat model, the albino rats were grouped into 2 of five (5) rats. The control group (1) was only given normal rat food and water while the test group (2) was treated with 0.4 g of industrial sugar/mL/body weight in addition to rat food and water via oral intubation for 21 days. Blood samples were collected via ocular puncture into plain tubes for testosterone measurement as described above. Results: There is a significant (p Conclusion: In conclusion, data available from this study suggests that increased plasma glucose levels and excessive industrial sugar consumption are associated with decreased testosterone levels in both human and animal models respectively. The mechanism is yet unclear but it could be that DM alters conventional sperm parameters, spermatogenesis, and biosynthesis of testosterone and induces degenerative changes in the testis and epididymis and ejaculatory dysfunction. These mechanisms need to be investigated.展开更多
In this comprehensive review,we delve into the evolution of drug delivery systems in reproductive medicine with a focus on the emerging role of exosomes,a class of extracellular vesicles.Exosomes offer unique advantag...In this comprehensive review,we delve into the evolution of drug delivery systems in reproductive medicine with a focus on the emerging role of exosomes,a class of extracellular vesicles.Exosomes offer unique advantages in overcoming these challenges due to their inherent biocompatibility,stability,and ability to facilitate targeted delivery.This review provides a detailed examination of exosome biogenesis and their function in cellular communication,setting the stage for understanding their potential as drug delivery vehicles.We explore the mechanisms through which exosomes can be loaded with small molecule drugs and the benefits they offer over synthetic nanoparticles.The review highlights groundbreaking case studies that illustrate the successful application of exosome-mediated drug delivery in reproductive health,including enhancing fertility treatments,supporting gamete and embryo development,and facilitating maternal-fetal communication.This study aims to provide a precise un-derstanding of how exosomal drug delivery can revolutionize treatments for reproductive health dis-orders,paving the way for future therapeutic applications.Lastly,we touch upon the promising therapeutic implications of exosomal delivery for proteins and genes,offering a window into future treatments for reproductive health disorders.展开更多
The biological species concept defines species as groups of actually or potentially interbreeding natural populations that are reproductively isolated from other such groups(Mayr,1942).Reproductive isolation,whether p...The biological species concept defines species as groups of actually or potentially interbreeding natural populations that are reproductively isolated from other such groups(Mayr,1942).Reproductive isolation,whether prezygotic or postzygotic,plays a central role in maintaining species boundaries.However,hybridization between closely related taxa can challenge these boundaries and provide insight into speciation,gene flow,and evolutionary processes(Coyne and Orr,2004).展开更多
This is a correction to:Isabela R R Moraes,Mariana Antunes,Laura S Lopez-Greco,Fernando Jose Zara,Antonio Leao Castilho,Functional reproductive morphology of the snapping shrimp genus Synalpheus Spence Bate,1888(Decap...This is a correction to:Isabela R R Moraes,Mariana Antunes,Laura S Lopez-Greco,Fernando Jose Zara,Antonio Leao Castilho,Functional reproductive morphology of the snapping shrimp genus Synalpheus Spence Bate,1888(Decapoda,Alpheidae),Current Zoology,2024,71,338-352;https:/doi.org/10.1093/cz/zoae053.展开更多
Studying the reproductive system of the pine wood nematode,Bursaphelenchus xylophilus(Steiner&Buhrer)Nickle,will identify its characteristics and life cycle.This is crucial for developing more targeted control str...Studying the reproductive system of the pine wood nematode,Bursaphelenchus xylophilus(Steiner&Buhrer)Nickle,will identify its characteristics and life cycle.This is crucial for developing more targeted control strategies.In this study,the development of the gonads and reproductive organs were observed using microscopy,gonad dissection,and DAPI staining techniques.Second-stage juveniles(J2)had gonads composed of four primordial germ cells(Z1,Z2,Z3,and Z4)that form the adult gonads by proliferation within 72 h at 25℃.There were subtle differences in somatic gonad cell morphology between males and females,which developed from Z1 and Z4 in third-stage juveniles(J3).These differences became more pronounced at fourth-stage juveniles(J4)and adult stages.Z2 and Z3 germ cells underwent mitosis and two rounds of meiosis,ultimately developing into male and female gametes.Female vulval precursor cells and male cloacal cells developed rapidly during the J4 stage.These results provide a basis for identifying the expression sites and biological functions of key genes regulating reproductive system development.Based on this,in situ hybridization and RNA interference(RNAi)were used to determine the function of the Bxy-glp-1 gene to show that it is involved in vulval formation and spermatogenesis.The results of this study will lay the foundation for disrupting critical stages in the reproduction of B.xylophilus.展开更多
Antarctic krill(Euphausia superba)is a keystone species in the Southern Ocean;however,seasonal variations in reproductive development for krill are complex and remains unknown.A histological investigation with observa...Antarctic krill(Euphausia superba)is a keystone species in the Southern Ocean;however,seasonal variations in reproductive development for krill are complex and remains unknown.A histological investigation with observations of external secondary sex features of krill in the south Scotia Sea region was carried out using a multi-seasonal dataset for detail reproductive descriptions for this commercially important species.The monthly development of secondary sexual characteristics,the thelycum,as well as of oocytes over a period of two years were described.It was observed that krill have diverse reproductive development characteristics within the ovary,and that this function differently between the juvenile,sub-adult,and adult stages.During the summer,adult krill ovaries are large and ripe with oocytes rich in yolk ready for release in late summer.Post spawning,the ovaries resorb,fragment,and regress throughout the autumn and winter.During reproductive diapause period,krill focus on absorbing nutrients.Un-released eggs are reabsorbed by the ovary,the permanent germinal zone is active,and early oocytes begin to develop in preparation for the egg production phase.Krill that are about to spawn have an ovary that fills the space between the digestive gland and muscle.The ovarian development of krill is divided into 10 sexual developmental stages.As a part of this study,data on the carapace thickness with similar development patterns in krill size and carapace width,was investigated for the first time to help understand krill growth and development.展开更多
Animal coloration has a wide range of biological functions and may be subject to different,sometimes conficting,selective pressures.In crustaceans,the evolution of coloration is relatively unstudied,despite the broad ...Animal coloration has a wide range of biological functions and may be subject to different,sometimes conficting,selective pressures.In crustaceans,the evolution of coloration is relatively unstudied,despite the broad range of colors and color patterns,which includes variability at multiple levels.Freshwater crayfsh are known to show color variability within species and populations,as well as intra-individual variability,but thefunction,if any,of crayfsh coloration is largely unknown.Here,I report on an experiment to understand patterns of color variability in the crayfsh Faxonius virilis and show that variation is strongly correlated to ontogenetic changes from a summer non-reproductive form to a fall reproductiveform.Crayfsh showed comparatively little inter-and intra-individual color variation in their non-reproductive form,but substantial variation at bothlevels in the reproductive form.Transition to the reproductive form was associated with the development of greener or bluer coloration localizedto the chelae on a subset of individuals,but these changes showed no clear correlation with sex or body size.Future investigations should focuson determining whether differences in color between individuals in the mating season are associated with any physiological or behavioral differences,or with differential susceptibility to predation.展开更多
基金supported by the National Natural Science Foundation of China(U23A20160,32360336)Guizhou Provincial Key Technology R&D Program(Qian KeHe ZhiCheng[2023]YiBan035).
文摘Natural hybridization is known to play a vital role in speciation;however,the mechanisms underlying the early stages of natural hybridization remain unclear.Where two plant species come into contact,two driving forces may balance the dynamic consequences of hybridization:fusion by hybridization-mediated gene flow,and separation by reproductive isolation(RI)(Ma et al.,2010a,b;Chang et al.,2022).
基金supported and funded by the National Key Research and Development Program of China(2023YFD1300801)the Agricultural Science and Technology Innovation Program in Chinese Academy of Agricultural Sciences(ASTIP-IAS-08)。
文摘Background The decline in reproductive performance of aged hens is mainly attributed to oxidative damage in reproductive organs,hepatic lipid metabolism disorders,and intestinal microbiota dysbiosis.Glycyrrhizin(GL)has been proven to enhance antioxidant capacity,regulate lipid metabolism and gut microbiota in mammals,but its efficacy in hens remains unclear.Hence,this study aimed to investigate whether dietary GL supplementation improves reproductive performance in hens during the late laying stage by modulating intestinal microbiota composition,hepatic lipid metabolism and ovarian antioxidant status.Results Dietary supplementation with 100 mg/kg GL significantly improved the egg production rate,egg quality,and hatching rate in aged breeder hens(P<0.05).GL supplementation also increased the serum levels of HDLC,TP and ALB,and enhanced the antioxidant capacity in both serum and ovary(P<0.05).In addition,dietary GL elevated the serum progesterone(P4)levels by enhancing the transcription level of steroid synthesis key enzymes(CYP11A1 and 3β-HSD)in the ovary(P<0.05).Dietary GL also promoted the synthesis and transport of vitellogenin(VTG)by upregulating the VTG-Ⅱ(P<0.05)and APOV1(P=0.077)expression levels in the liver,thereby increasing the number of grade follicles and small yellow follicles.Moreover,dietary GL enhanced hepatic fatty acidβ-oxidation by upregulating PPARαand CPT-I(P<0.05),and downregulating ACC expression levels(P<0.05).In agreement,liver metabolomics analysis revealed that dietary GL supplementation significantly altered hepatic metabolism,with 389 differentially identified metabolites(P<0.05).The key metabolites(e.g.,taurocholic acid,tauroursodeoxycholic acid,nicotinuric acid,glycodeoxycholic acid(hydrate))were identified,and they were mainly functionally enriched in betaalanine metabolism nicotinate,taurine and hypotaurine metabolism(P<0.05).Finally,16S rRNA gene sequencing revealed that dietary GL reversed age-induced changes in gut microbiota composition,characterized by a significant increase in Lactobacillus abundance and a decrease in Bacteroides(P<0.05).Conclusions These results collectively demonstrate that dietary supplementation with 100 mg/kg GL improved reproductive performance by reversing age-induced changes in gut microbiota,enhancing hepatic vitellogenin synthesis,and ameliorating ovarian function in aged breeder hens.This study suggests that dietary GL is a potential strategy to improve reproductive performance in broiler breeder hens during the late laying period.
文摘Background:Epidemiological studies have confirmed that longer exposure to insecticides like cypermethrin(CYP)significantly increases the risk of male reproductive toxicity.Crocus sativus L.has been recognized due to its therapeutic properties,but its exact role and molecular mechanisms in treatment of reproductive dysfunction remain unclear.Methods:During this study,36 rats were randomly divided into six groups(n=6):control,CYP-induced(60 mg/kg),standard(leuprolide 3 mg/kg)and three treatment groups receiving aqueous,ethanolic,and oil extracts(50 mg/kg or 20 mL/kg)for post-toxicity induction.Results:The finding represented that exposure of CYP significantly increased oxidative stress,disrupted testicular architecture,and markedly reduced testosterone levels(P<0.05).Importantly,Crocus sativus L.treatment alleviated these changes by increasing the expression of Nrf2(nuclear factor erythroid 2-related factor 2),restoring the activity of antioxidant enzymes,and enhancing testicular histomorphology.Surprisingly,molecular docking established a high binding affinity of Crocus sativus L.phytoconstituents such as gallic acid,cinnamic acid and quercetin to the Nrf2-Keap1 complex.It is worth noting that,Crocus sativus L.exhibited a high level of protection against reproductive toxicity caused by CYP in male rats,which was mediated by the activation of Nrf2 pathway,reduction of oxidative damage,and favorable ADMET characteristics.Conclusion:Notably,this research provides a more valid,safe,and effective method of developing new drugs for reproductive disorders,however,further investigation is needed to support the research findings and implement it in clinical practice.
文摘Elaeagnaceae Juss. belongs to the family Elaeagnus L. and Shepherdia Nutt. This study aims at the significance and vegetative reproduction of species of the family (Elaeagnus macrophylla Thunb. and Shepherdia canadensis (L.) Nutt.) in the national economy. The trials were carried out in the experimental site of SamSU’s Botanic Garden, which is named after Sharof Rashidov. The study’s findings are based on the results obtained by planting cuttings of S. canadensis and E. macrophylla species of varying sizes and durations, as well as the cuttings’ roots and the impacts of growth chemicals.
文摘Paris polyphylla Smith is an important medicinal plant that is considered vulnerable in many parts of the world. The species exhibits poor adaptability to changed environment and susceptible to climatic conditions for reproductive vigor. During the present study the species was studied in four different natural niches as well as synthetic habitats to check the adaptability to artificial conditions, its morphological and physiological characters and its interaction in its community so that conservation strategies could be worked out. The present study indicates that P. polyphylla exhibits healthy growth as well as reproduction in undisturbed area with canopy covers over 80%. The plant grows in well-drained rich humus soil and grows well with certain key associated species such as Quercus species, Taxus baccata, Aconitum species, Eupatorium adenophorum, Smilax species etc. Many plants were non-flowering and more than 60% were found non-flowering in its habitat during its flowering season in any particular year. There are clear morphological changes as the flowers stages from pre-fertilization to post-fertilization. Arrangement of anthers in two whorls shows a distinct and unique floral display. Light is an important factor during seed setting and less than 50% shade lowers the seed productivity very significantly. Predators such as slugs and snails are found on the plant. Urgent conservation and new innovative ways are necessary to propagate this economically important plant to ensure its continuity.
基金Thisworkwas supported by the National Natural Science Foundation of China(32222074,32072551)the National Key Research and Development Program of China(2022YFD2100102-3)+2 种基金111 Project(B17043)Beijing Innovation Consortium of Agriculture Research System(BAIC04-2022)the 2115 Talent Development Program of China Agricultural University.
文摘The WRKY transcription factors play important roles in plant growth and resistance,but only a few members have been identified in strawberry.Here we identified a WRKY transcription factor,FvWRKY50,in diploid strawberry which played essential roles in strawberry vegetative growth,and reproductive growth.Knocking out FvWRKY50 by genome editing accelerated flowering time and leaf senescence but delayed anthocyanin accumulation in fruit.Further analysis showed that FvWRKY50 acted as a transcriptional repressor to negatively regulate the expression of flowering-and leaf senescence-related genes,including FvFT2,FvCO,FvFT3,and FvSAUR36.Notably,FvWRKY50 directly upregulated the expression of FvCHI and FvDFR by binding their promoter under normal conditions,but at low temperature FvWRKY50 was phosphorylated by FvMAPK3 and then induced protein degradation by ubiquitination,delaying anthocyanin accumulation.In addition,the homozygous mutant of FvWRKY50 was smaller while the biallelic mutant showed normal size.These new findings provide important clues for us to further reveal the regulatory mechanisms of strawberry growth and fruit ripening.
文摘The efficacy of crude extracts of Senna alata in the improvement of vegetative and reproductive growth in Celosia aregentea was investigated. Fresh leaves of S. alata were blended with a homogenizer in 1 litre of distilled water. The resultant green paste was filtered under suction. Different concentrations (75%, 50%, 40%, 30%, 25%, 12%, 10%, and 5%) were prepared from the 100% crude extract. Seeds of C. argentea were presoaked in these different concentrations including a control (0%) and planted out after 24 hours. Results obtained showed that seedling height, leaf area, dry weight and leaf area ratio were promoted and enhanced by presoaking seeds in the extract. At the end of the experimental period (six weeks), seedling height in 75% and 100% treatments were 109 ± 16.12 cm and 117 ± 19.32 cm, leaf area 128 ± 17.91 cm2 and 125 ± 18.12 cm2, dry weight 7.48 kg and 7.0 kg respectively. Seedlings raised from seeds presoaked in water (control) however, flowered earlier (8 weeks) than the treatments (10 weeks in 75% and 100%). Presoaking seeds of C. argentea in crude extracts of S. alata before planting is recommended for optimum production of the leafy vegetable. The procedure is cheap and easily implementable by resource-poor farmers who are the main growers of C. argentea.
基金supported by the National Nature Science Foundation of China(NSFC,No.31960156,No.31660338,No.31760627,and No.32270848)Collaborative Innovation Center of Chinese Ministry of Education(2020-39),Science and Technology Support Program of Guizhou Province(QKH[2021]111,QKH[2020]4Y192,QKH[2018]5772-006,and QKH[2019]5406)Science and Technology Fund of Guizhou Provincial Health Commission(gzwkj2022-019).
文摘Compounds isolated from Epimedium include the total flavonoids of Epimedium,icariin,and its metabolites(icaritin,icariside Ⅰ,and icariside Ⅱ),which have similar molecular structures.Modern pharmacological research and clinical practice have proved that Epimedium and its active components have a wide range of pharmacological effects,especially in improving sexual function,hormone regulation,anti-osteoporosis,immune function regulation,anti-oxidation,and anti-tumor activity.To date,we still need a comprehensive source of knowledge about the pharmacological effects of Epimedium and its bioactive compounds on the male reproductive system.However,their actions in other tissues have been reviewed in recent years.This review critically focuses on the Epimedium,its bioactive compounds,and the biochemical and molecular mechanisms that modulate vital pathways associated with the male reproductive system.Such intrinsic knowledge will significantly further studies on the Epimedium and its bioactive compounds that protect the male reproductive system and provide some guidances for clinical treatment of related male reproductive disorders.
文摘BACKGROUND Breast cancer is one of the most prevalent causes of morbidity and mortality worldwide,presenting an increasing public health challenge,particularly in lowincome and middle-income countries.However,data on the knowledge,attitudes,and preventive practices regarding breast cancer and the associated factors among females in Wollo,Ethiopia,remain limited.AIM To assess the impact of family history(FH)of breast disease on knowledge,attitudes,and breast cancer preventive practices among reproductive-age females.METHODS A community-based cross-sectional study was conducted in May and June 2022 in Northeast Ethiopia and involved 143 reproductive-age females with FH of breast diseases and 209 without such a history.We selected participants using the systematic random sampling technique.We analyzed the data using Statistical Package for Social Science version 25 software,and logistic regression analysis was employed to determine odds ratios for variable associations,with statistical significance set at P<0.05.RESULTS Among participants with FH of breast diseases,the levels of knowledge,attitudes,and preventive practices were found to be 83.9%[95%confidence interval(CI):77.9-89.9],49.0%(95%CI:40.8-57.1),and 74.1%(95%CI:66.9-81.3),respectively.In contrast,among those without FH of breast diseases,these levels were significantly decreased to 10.5%(95%CI:6.4-14.7),32.1%(95%CI:25.7-38.4),and 16.7%(95%CI:11.7-21.8),respectively.This study also indicated that knowledge,attitudes,and preventive practices related to breast cancer are significantly higher among participants with FH of breast diseases compared to those without HF breast diseases.CONCLUSION Educational status,monthly income,and community health insurance were identified as significant factors associated with the levels of knowledge,attitudes,and preventive practices regarding breast cancer among reproductive-age females.
基金funded by the National Key Research and Development Program of China (2023YFE0124400)the Specific university discipline construction project (2023B10564001)+1 种基金grants administered by the Arkansas Biosciences Institute and the USDAa core grant (P20GM121293, proteogenomics core)。
文摘Background Sow longevity and reproductivity are essential in the modern swine industry.Although many studies have focused on the genetic and genomic factors for selection,little is known about the associations between the microbiome and sows with longevity in reproduction.Results In this study,we collected and sequenced rectal and vaginal swabs from 48 sows,nine of which completed up to four parities(U4P group),exhibiting reproductive longevity.We first identified predictors of sow longevity in the rectum(e.g.,Akkermansia)and vagina(e.g.,Lactobacillus)of the U4P group using RandomForest in the early breeding stage of the first parity.Interestingly,these bacteria in the U4P group showed decreased predicted KEGG gene abundance involved in the biosynthesis of amino acids.Then,we tracked the longitudinal changes of the micro-biome over four parities in the U4P sows.LEfSe analysis revealed parity-associated bacteria that existed in both the rectum and vagina(e.g.,Streptococcus in Parity 1,Lactobacillus in Parity 2,Veillonella in Parity 4).We also identi-fied patterns of bacterial change between the early breeding stage(d 0)and d 110,such as Streptococcus,which was decreased in all four parties.Furthermore,sows in the U4P group with longevity potential also showed better reproductive performance.Finally,we discovered bacterial predictors(e.g.,Prevotellaceae NK3B31 group)for the total number of piglets born throughout the four parities in both the rectum and vagina.Conclusions This study highlights how the rectal and vaginal microbiome in sows with longevity in reproduc-tion changes within four parities.The identification of parity-associated,pregnancy-related,and reproductive performance-correlated bacteria provides the foundation for targeted microbiome modulation to improve animal production.
文摘Stromal vascular fraction(SVF)is a complex mixture derived from adipose tissue,consisting of a variety of cells.Due to its potential for tissue repair,immunomod-ulation,and support of angiogenesis,SVF represents a promising frontier in regenerative medicine and offers potential therapy for a range of disease condi-tions.In this article,we delve into the mechanisms through which SVF exerts its effects and explore its potential applications in treating both male and female reproductive disorders,including erectile dysfunction,testicular injury,stress urinary incontinence and intrauterine adhesion.
基金supported by the Guangdong Provincial Key Area Research and Development Program[grant number 2022B0202090002]China Postdoctoral Science Foundation[grant number 2024M760977].
文摘Background Hexafluoropropylene oxide dimer acid(GenX),a substitute for per-and polyfluoroalkyl substances,has been widely detected in various environmental matrices and foods recently,attracting great attention.However,a systematic characterization of its reproductive toxicity is still missing.This study aims to explore the male reproductive toxicity caused by GenX exposure and the potential cellular and molecular regulatory mechanisms behind it.Results Normally developing mice were exposed to GenX,and testicular tissue was subsequently analyzed and validated using single-cell RNA sequencing.Our results revealed that GenX induced severe testicular damage,disrupted the balance between undifferentiated and differentiated spermatogonial stem cells,and led to strong variation in the cellular dynamics of spermatogenesis.Furthermore,GenX exposure caused global upregulation of testicular somatic cellular inflammatory responses,increased abnormal macrophage differentiation,and attenuated fibroblast adhesion,disorganizing the somatic-germline interactions.Conclusions In conclusion,this study revealed complex cellular dynamics and transcriptome changes in mouse testis after GenX exposure,providing a valuable resource for understanding its reproductive toxicity.
基金The University of Winnipeg and The University of Winnipeg Foundation contributed funding to this research.
文摘Variation in the vocal behavior of nonhuman vertebrates includes graded transitions and more dramatic changes.Wapiti males produce a reproductive bugle that has a fundamental frequency that surpasses 2,000 Hz with evidence of biphonation and other nonlinear phenomena.Here,we analyze the acoustic structure of captive wapiti vocalizations to compare the male bugle with 3 categories of distress vocalizations:neonate distress(capture)calls,calf isolation calls,and adult female isolation calls.These 4 high-arousal call categories serve a common general function in recruiting conspecifics but occur in different behavioral contexts(capture,isolation,reproduction).Our goal was to distinguish characteristics that vary in graded steps that may correspond to an animal’s age or size from characteristics that are unique to the bugle.Characteristics of the high and loud fundamental(G0)varied in an age/size-graded manner with a decrease in minimum G0,an increase in the maximum and range of G0,with no evidence of sex differences.The nonlinear phenomena of deterministic chaos,biphonation,and frequency jumps were present in all 4 call categories and became more common from the distress vocalizations of neonates to calves to adult females to the male bugle.Two temporal characteristics sharply distinguished the bugle from the 3 categories of distress vocalizations:these included a prolonged call duration and a maximum G0 that occurred much later in the call for the bugle than for distress vocalizations.Our results suggest that distress vocalizations of different age groups and the reproductive bugle of wapiti share a high G0,with age/size-graded changes in G0 and nonlinear phenomena,but differ sharply in temporal characteristics.
文摘Background: Diabetes is a set of metabolic abnormalities typified by hyperglycemia believed to be associated with aberrant insulin secretion, insulin action, or both. Erectile dysfunction and infertility are globally prevalent complications among male diabetic patients. There is a need to understand the role of a male reproductive hormone in the etiology of infertility in diabetic patients. Objective: This study aims to determine the effect of diabetes mellitus on the Male reproductive hormone (testosterone) on diabetic patients attending the Urology unit of Federal University Teaching Hospital, Abakaliki, Ebonyi State, Nigeria and the effect of industrial sugar consumption in rats model. Materials and Methods: 30 samples (including repeats, n = 3) were collected from known diabetic patients and 30 (including repeats, n = 3) control-non diabetic healthy individuals. Five millilitres (5 ml) of blood was collected from the antecubital vein of each participant and placed into a plain container for serum testosterone measurement using the enzyme-linked immunoassay technique. Blood was also collected from the same subjects in a fluoride oxalate container for plasma enzymatic glucose estimation using a Randox Glucose oxidase kit. For in vivo rat model, the albino rats were grouped into 2 of five (5) rats. The control group (1) was only given normal rat food and water while the test group (2) was treated with 0.4 g of industrial sugar/mL/body weight in addition to rat food and water via oral intubation for 21 days. Blood samples were collected via ocular puncture into plain tubes for testosterone measurement as described above. Results: There is a significant (p Conclusion: In conclusion, data available from this study suggests that increased plasma glucose levels and excessive industrial sugar consumption are associated with decreased testosterone levels in both human and animal models respectively. The mechanism is yet unclear but it could be that DM alters conventional sperm parameters, spermatogenesis, and biosynthesis of testosterone and induces degenerative changes in the testis and epididymis and ejaculatory dysfunction. These mechanisms need to be investigated.
基金NGS assistant and Beijing Chipstone co.for their copy editing service.
文摘In this comprehensive review,we delve into the evolution of drug delivery systems in reproductive medicine with a focus on the emerging role of exosomes,a class of extracellular vesicles.Exosomes offer unique advantages in overcoming these challenges due to their inherent biocompatibility,stability,and ability to facilitate targeted delivery.This review provides a detailed examination of exosome biogenesis and their function in cellular communication,setting the stage for understanding their potential as drug delivery vehicles.We explore the mechanisms through which exosomes can be loaded with small molecule drugs and the benefits they offer over synthetic nanoparticles.The review highlights groundbreaking case studies that illustrate the successful application of exosome-mediated drug delivery in reproductive health,including enhancing fertility treatments,supporting gamete and embryo development,and facilitating maternal-fetal communication.This study aims to provide a precise un-derstanding of how exosomal drug delivery can revolutionize treatments for reproductive health dis-orders,paving the way for future therapeutic applications.Lastly,we touch upon the promising therapeutic implications of exosomal delivery for proteins and genes,offering a window into future treatments for reproductive health disorders.
基金supported by the National Natural Science Foundation of China(No.32161143024,31970405)Iran National Science Foundation,Iran-China(INSF-NSFC)joint project(No.4002006).
文摘The biological species concept defines species as groups of actually or potentially interbreeding natural populations that are reproductively isolated from other such groups(Mayr,1942).Reproductive isolation,whether prezygotic or postzygotic,plays a central role in maintaining species boundaries.However,hybridization between closely related taxa can challenge these boundaries and provide insight into speciation,gene flow,and evolutionary processes(Coyne and Orr,2004).
文摘This is a correction to:Isabela R R Moraes,Mariana Antunes,Laura S Lopez-Greco,Fernando Jose Zara,Antonio Leao Castilho,Functional reproductive morphology of the snapping shrimp genus Synalpheus Spence Bate,1888(Decapoda,Alpheidae),Current Zoology,2024,71,338-352;https:/doi.org/10.1093/cz/zoae053.
基金supported by grants from the National Natural Science Foundation of China(NSFC)to Jiafu Hu(grant no.32371892,31670652,and 31870633)Hudie Shao(grant no.32401591).
文摘Studying the reproductive system of the pine wood nematode,Bursaphelenchus xylophilus(Steiner&Buhrer)Nickle,will identify its characteristics and life cycle.This is crucial for developing more targeted control strategies.In this study,the development of the gonads and reproductive organs were observed using microscopy,gonad dissection,and DAPI staining techniques.Second-stage juveniles(J2)had gonads composed of four primordial germ cells(Z1,Z2,Z3,and Z4)that form the adult gonads by proliferation within 72 h at 25℃.There were subtle differences in somatic gonad cell morphology between males and females,which developed from Z1 and Z4 in third-stage juveniles(J3).These differences became more pronounced at fourth-stage juveniles(J4)and adult stages.Z2 and Z3 germ cells underwent mitosis and two rounds of meiosis,ultimately developing into male and female gametes.Female vulval precursor cells and male cloacal cells developed rapidly during the J4 stage.These results provide a basis for identifying the expression sites and biological functions of key genes regulating reproductive system development.Based on this,in situ hybridization and RNA interference(RNAi)were used to determine the function of the Bxy-glp-1 gene to show that it is involved in vulval formation and spermatogenesis.The results of this study will lay the foundation for disrupting critical stages in the reproduction of B.xylophilus.
基金Supported by the Inter-governmental Science and Technology Innovation(STI)Cooperation Special Program of the National Key Research and Development Program of China(No.2023YFE0104500)the NIWA-SHOU-Otago Joint Research Center on Antarctic Marine Science。
文摘Antarctic krill(Euphausia superba)is a keystone species in the Southern Ocean;however,seasonal variations in reproductive development for krill are complex and remains unknown.A histological investigation with observations of external secondary sex features of krill in the south Scotia Sea region was carried out using a multi-seasonal dataset for detail reproductive descriptions for this commercially important species.The monthly development of secondary sexual characteristics,the thelycum,as well as of oocytes over a period of two years were described.It was observed that krill have diverse reproductive development characteristics within the ovary,and that this function differently between the juvenile,sub-adult,and adult stages.During the summer,adult krill ovaries are large and ripe with oocytes rich in yolk ready for release in late summer.Post spawning,the ovaries resorb,fragment,and regress throughout the autumn and winter.During reproductive diapause period,krill focus on absorbing nutrients.Un-released eggs are reabsorbed by the ovary,the permanent germinal zone is active,and early oocytes begin to develop in preparation for the egg production phase.Krill that are about to spawn have an ovary that fills the space between the digestive gland and muscle.The ovarian development of krill is divided into 10 sexual developmental stages.As a part of this study,data on the carapace thickness with similar development patterns in krill size and carapace width,was investigated for the first time to help understand krill growth and development.
文摘Animal coloration has a wide range of biological functions and may be subject to different,sometimes conficting,selective pressures.In crustaceans,the evolution of coloration is relatively unstudied,despite the broad range of colors and color patterns,which includes variability at multiple levels.Freshwater crayfsh are known to show color variability within species and populations,as well as intra-individual variability,but thefunction,if any,of crayfsh coloration is largely unknown.Here,I report on an experiment to understand patterns of color variability in the crayfsh Faxonius virilis and show that variation is strongly correlated to ontogenetic changes from a summer non-reproductive form to a fall reproductiveform.Crayfsh showed comparatively little inter-and intra-individual color variation in their non-reproductive form,but substantial variation at bothlevels in the reproductive form.Transition to the reproductive form was associated with the development of greener or bluer coloration localizedto the chelae on a subset of individuals,but these changes showed no clear correlation with sex or body size.Future investigations should focuson determining whether differences in color between individuals in the mating season are associated with any physiological or behavioral differences,or with differential susceptibility to predation.
