Elaeagnaceae Juss. belongs to the family Elaeagnus L. and Shepherdia Nutt. This study aims at the significance and vegetative reproduction of species of the family (Elaeagnus macrophylla Thunb. and Shepherdia canadens...Elaeagnaceae Juss. belongs to the family Elaeagnus L. and Shepherdia Nutt. This study aims at the significance and vegetative reproduction of species of the family (Elaeagnus macrophylla Thunb. and Shepherdia canadensis (L.) Nutt.) in the national economy. The trials were carried out in the experimental site of SamSU’s Botanic Garden, which is named after Sharof Rashidov. The study’s findings are based on the results obtained by planting cuttings of S. canadensis and E. macrophylla species of varying sizes and durations, as well as the cuttings’ roots and the impacts of growth chemicals.展开更多
Leaves are the main organ for photosynthesis and organic synthesis in cotton.Leaf shape has important effects on photosynthetic efficiency and canopy formation,thereby affecting cotton yield.Previous studies have show...Leaves are the main organ for photosynthesis and organic synthesis in cotton.Leaf shape has important effects on photosynthetic efficiency and canopy formation,thereby affecting cotton yield.Previous studies have shown that LMI1(LATE MERISTEM IDENTITY1)is the main gene regulating leaf shape.In this study,the LMI1 gene was inserted into the 35S promoter expression vector,and cotton plants overexpressing LMI1(OE)were obtained through genetic transformation.Statistical analysis of the biological traits of the T_(1) and T_(2) populations showed that compared to the wild type(WT),OE plants had significantly larger leaves,thicker stems and significantly greater dry weight.Furthermore,plant sections of the main vein and petiole showed that the numbers of cells in those tissues of OE plants were significantly greater.In addition,RNA-seq analysis revealed the differential expression of genes related to gibberellin synthesis and NAC gene family(genes containing the NAC domain)between the OE and WT plants,suggesting that LMI1 is involved in secondary wall formation and cell proliferation,which promotes stem thickening.Moreover,Gene Ontology(GO)analysis revealed enrichment in the terms of calcium ion binding,and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis showed enrichment in the terms of fatty acid degradation,phosphatidylinositol signal transduction system,and c AMP(cyclic adenosine monophosphate)signal pathway.These results suggested that LMI1 OE plants are responsive to gibberellin hormone signals,and have altered messenger signals(c AMP,Ca^(2+))which amplify this function,to promote stronger aboveground vegetative growth.This study found the LMI1 greatly increased the vegetative growth in cotton,which is the basic requirement for higher yield.展开更多
The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. ...The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. Endogenous IAA, DHZ+DHZR, iPA increased significantly during the floral bud formation, while Z+ZR remained changed. The levels of cytokinins, DHZ+DHZR, iPA, and Z+ZR all increased significantly during the vegetative bud formation, however IAA level was reduced during the first 7 days of culture and increased to two thirds of initial values on the day when the bud primordia were formed. The results suggested that the initiation of floral buds was associated with a high IAA/CTK ratio, whereas the induction of vegetative bud differentiation was related to a low IAA/CTK ratio.展开更多
The ultrastructure of the vegetative cells of Nostoc flagelliforme Born. et Flah. was investigated with high pressure freezing and freeze substitution technique and compared with the results obtained by using conv...The ultrastructure of the vegetative cells of Nostoc flagelliforme Born. et Flah. was investigated with high pressure freezing and freeze substitution technique and compared with the results obtained by using conventional preparation methods. During the processes of chemical fixation, dehydration and embedding, the cell structures might be more artificially modified than that obtained from high pressure freezing and freeze substitution. With the present method, the sheath of N. flagelliforme could be well penetrated and no extra big space could exist between the cell and the sheath. The cell protoplasm rarely shrinked. Some fine structures of cell inclusions and unit membranes became visualized. Many bacteria were harbored in the sheath. In addition, the presence of big vacuoles in the cell of N. flagelliforme as well as the presence of bacteria in the sheath shown in the present preparation for cyanobacteria has not been described so far in the literature.展开更多
[Objective] This study aimed to identify possible quantitative trait loci (QTL) for vegetative drought tolerance traits of rice (Oryza sativa L.). [Method] A total of 184 rice genotypes were field screened and 156 SSR...[Objective] This study aimed to identify possible quantitative trait loci (QTL) for vegetative drought tolerance traits of rice (Oryza sativa L.). [Method] A total of 184 rice genotypes were field screened and 156 SSR markers randomly distributed at each 3 Mb bin were used through genome-wide scanning method and association analysis to detect QTLs for vegetative drought tolerance traits leaf rolling (LER), leaf drying (LED) and drought recovery rate (DRR). [Result] The experimental rice genotypes showed varied response under vegetative drought, LER, LED and DRR were highly correlated. Population structure was detected at K=3 and K=7, certain extent of admixture existed in the experimental rice genotypes, relative kinship of the rice genotypes ranged from 0 to 0.924 5. Significant linkage disequilibrium among SSR markers was detected. Sixteen SSR markers have been detected to be associated with vegetative drought tolerance traits, four for LER, eight for LED and four for DDR. Most of the markers were associated with more than one trait, indicating a single mechanism might involve in expression of several related traits, i.e. osmotic adjustment. RM107 (Chr.9) was associated with all the three traits and fell exactly within or closely nearby to previous reported regions, was a major QTL for vegetative drought tolerance, RM477 (Chr.8) was significantly associated with DRR and extremely significantly associated with LER was probably another major QTL for vegetative drought tolerance. [Conclusion] Association mapping is a very effective method for describing complex traits like drought tolerance.展开更多
Structural and organizational changes of the vegetative cell in Amaryllis vittata Ait. during such dynamic processes of pollen as hydration, activation and germination have been examined with electron microscopy. The ...Structural and organizational changes of the vegetative cell in Amaryllis vittata Ait. during such dynamic processes of pollen as hydration, activation and germination have been examined with electron microscopy. The mature pollen grain is composed of such organelles as plastids, mitochondria,endoplasmic reticulum, dictyosomes and lipid bodies which are in their resting state. Microfilaments appear as aggregates. After pollen activation, however,the organelles undergo great changes in number and shape: the lamellae of plastids and the cristae of mitochondria increase conspicuously in number, the cisternae of the endoplasmic reticulum become narrower; the dictyosomes produce vesicles actively the lipid bodies become degraded and the microfilament aggregates disperse. Cortical microtubules and spiny vesicles appear in the cytoplasm after germination of the pollen tube. No apparent structural changes of the organelles were noticed any longer during this period and microfilaments are distributed throughout the entire pollen tube as a three-dimensional network.展开更多
Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid （IAA） @ 2000, 4000 （ppm）, indolebutyric acid （IBA） @ 2000, 4000 （ppm） and naphthlcetic acid...Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid （IAA） @ 2000, 4000 （ppm）, indolebutyric acid （IBA） @ 2000, 4000 （ppm） and naphthlcetic acid （NAA） @ 2000, 4000 （ppm） in dry formulation in the Forest Nursery, Faculty of Forestry, SKUAST-K, Shalimar. The cuttings treated with IBA @ 4000 ppm and IBA @ 2000 ppm had a sprouting rate of 75% and 50%, respectively, which was significantly higher than that of control and other treatments. The highest rooting rate （50%） was recorded in the cuttings with the application of IBA @ 4000 ppm. The cuttings treated with IBA @ 2000 ppm had 25% rooting rate. All other treatments along with control （talc powder） failed to induce rooting. It was concluded that IBA @ 4000 ppm was a better-applied concentration for vegetative propagation ofA. indica under Kashmir conditions.展开更多
Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid(IAA) @ 2000, 4000(ppm), indolebutyric acid(IBA) @ 2000, 4000(ppm) and naphthlcetic acid(NAA) @ 2000...Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid(IAA) @ 2000, 4000(ppm), indolebutyric acid(IBA) @ 2000, 4000(ppm) and naphthlcetic acid(NAA) @ 2000, 4000(ppm) in dry formulation in the Forest Nursery, Faculty of Forestry, SKUAST-K, Shalimar.The cuttings treated with IBA @ 4000 ppm and IBA @ 2000 ppm had a sprouting rate of 75% and 50%, respectively, which was significantly higher than that of control and other treatments.The highest rooting rate(50%) was recorded in the cuttings with the application of IBA @ 4000 ppm.The cuttings treated with IBA @ 2000 ppm had 25% rooting rate.All other treatments along with control(talc powder) failed to induce rooting.It was concluded that IBA @ 4000 ppm was a better-applied concentration for vegetative propagation of A.indica under Kashmir conditions.展开更多
Vegetative filter strip (VFS) is a main kind of Best Management Practices for the control of non-point source pollution. The goal of this paper is to evaluate the effectiveness of VFS in Chinese northwest regions. Thr...Vegetative filter strip (VFS) is a main kind of Best Management Practices for the control of non-point source pollution. The goal of this paper is to evaluate the effectiveness of VFS in Chinese northwest regions. Three VFSs with natural grass and Hippophae rhamnoides/grass patterns have been constructed in the bank slope of Xiaohuashan reservoir, Huaxian County, Shannxi Province. The removal effects of VFS and influencing factors have been analyzed based on field experiment data. The result reveals a positive effect on reducing the transportation of suspended solids, phosphorus and nitrogen in surface runoff, and it is more efficient on suspended solids removal. The experiment also shows that most of the suspended particles and pollutants bound to them were entrapped in the first 10 m of VFS. The main factors influencing effectiveness of VFS include vegetation patterns and inflow rate. In addition, inflow pollutant concentration has a larger impact on reducing total nitrogen and total phosphorus by VFS, but the reduction effect on SS has no significant difference.展开更多
The ultrastructural characteristics and the subcellular localization of vegetative storage proteins(VSPs)of Ginkgo biloba L.were systematically studied under the electron microscope.Results indicated that the VSPs of ...The ultrastructural characteristics and the subcellular localization of vegetative storage proteins(VSPs)of Ginkgo biloba L.were systematically studied under the electron microscope.Results indicated that the VSPs of G.biloba were mostly distributed in the small vacuoles of the phloem parenchyma cells.The VSPs of phloem parenchyma cells were produced in cytoplasm,then separated by the inflated cisternae of endoplasmic reticulum(ER),plasmalemma invagination or Golgi body vesicles,resulting in the formation of vacuole filled with proteins.Three kinds of VSPs were detected:granular,floccular and massive VSPs,which were distributed in different tissue cells and different cells of the same tissue or vacuoles of the same cells.VSPs accumulated in autumn and kept at high level throughout the winter.In the following spring,the bud started growing and VSPs were completely mobilized.With new shoots growth,VSPs resumed accumulating in late summer and early autumn.展开更多
For the first time, the anatomical structure of the vegetative organs of the species J. hippolyti and J. narbuti was studied in the conditions of Kyzylkum (Uzbekistan, Province Bukhara) and revealed the diagnostic fea...For the first time, the anatomical structure of the vegetative organs of the species J. hippolyti and J. narbuti was studied in the conditions of Kyzylkum (Uzbekistan, Province Bukhara) and revealed the diagnostic features of these species. A different combination of xeromorphic and mesomorphic features was determined, which ensures adaptation to habitat conditions. In J. narbuti xeromorphic features predominate, which indicates a more adaptable and wide distribution in the natural habitat of this species. In J. hippolyti—mesomorphic signs that show the rarity of the species in their natural habitat, compared to J. narbuti.展开更多
The vegetative state is a complex condition with unclear mechanisms and limited diagnostic, prognostic, and therapeutic methods. In this study, we aimed to explore the proteomic profile of tears from patients in a tra...The vegetative state is a complex condition with unclear mechanisms and limited diagnostic, prognostic, and therapeutic methods. In this study, we aimed to explore the proteomic profile of tears from patients in a traumatic vegetative state and identify potential diagnostic markers using tears-a body fluid that can be collected noninvasively. Using iTRAQ quantitative proteomic technology, in the discovery phase, tear samples collected from 16 patients in a traumatic vegetative state and 16 normal individuals were analyzed. Among 1080 identified tear proteins, 57 were upregulated and 15 were downregulated in the patients compared to the controls. Bioinformatics analysis revealed that the differentially-expressed proteins were mainly involved in the wound response and immune response signaling pathways. Furthermore, we verified the levels of 7 differentially-expressed proteins in tears from 50 traumatic vegetative state patients and 50 normal controls （including the samples used in the discovery phase） using ELISA. The results showed that this 7-protein panel had a high discrimination ability for traumatic vegetative state （area under the curve = 0.999）. In sum- mary, the altered tear proteomic profile identified in this study provides a basis for potential tear protein markers for diagnosis and prognosis of the traumatic vegetative state and also provides novel insights into the mechanisms of traumatic vegetative state.展开更多
The anatomical structures of roots, stems and leaves are very important indicators to＇evaluate drought resistance. The paper reports the relationship between anatomical structures of vegetative organs and drought res...The anatomical structures of roots, stems and leaves are very important indicators to＇evaluate drought resistance. The paper reports the relationship between anatomical structures of vegetative organs and drought resistance in forages.展开更多
High temperature stress （HT） is efficient in breaking endo-dormancy of perennial trees. The effects of HT （50°C） on the respiration of dormant nectarine （Prunus persica var. nectariana cv. Shuguang） vegetat...High temperature stress （HT） is efficient in breaking endo-dormancy of perennial trees. The effects of HT （50°C） on the respiration of dormant nectarine （Prunus persica var. nectariana cv. Shuguang） vegetative buds were evaluated in the research. We found that bud respiration was transiently inhibited by HT and the pentose phosphate pathway （PPP） and the cytochrome C pathway （CYT） were significantly affected. On the substrate level, PPP was activated in the HT-treated buds compared with the control group. However, the activation did mot occur until hours after HT treatment. The tricarboxylic acid cycle （TCA） in both the HT-treated buds and in the control group proceeded at a low level most of the time compared with total respiration. On the electron transfer level, CYT was transiently inhibited by HT but became significantly active in the later stage. CYT operation in the control group exhibited an attenuation process. The alternative pathway （ALT） fluctuated both in the HT-treated samples and in the control. The results suggest that the temporary CYT inhibition and the following PPP activation may be involved in HT-induced bud dormancy release and budburst mechanisms.展开更多
In this paper, the traits of immature embryo culture from 45 genotypes of bread wheat were investigated in detail, and trait correlation analysis in combination with histomorphological examination was used to study th...In this paper, the traits of immature embryo culture from 45 genotypes of bread wheat were investigated in detail, and trait correlation analysis in combination with histomorphological examination was used to study the cytological origin of flower as well as the correlation between it and vegetative shoot development from the calli. The rate of floral development in the subculture was highly significantly and positively related to that of the differentiation culture, which reveals that the genetic system of floral development from immature embryo-derived calli in wheat was highly correspondent and stable. The rate of floral development was related neither to the traits in the dedifferentiation culture, nor to the vegetative shoot formation from the total induced calli, but significantly and positively related to that of the calli without leaf-like green spots in the differentiation culture. These findings indicate the partial correlation as well as differentiation between flower and vegetative shoot development. The floral development might only be associated with the genetic system controlling organ regeneration, while vegetative shoots formed from the two ways, including somatic embryogenesis and organ regeneration.展开更多
Vegetative growth of young apple trees trained in vertical axis were studied with ' Red Fuji', 'Jonagold', 'Orin' and ' Starkrimson' on M7, MM106, M26 interstocks in northern China. Abo...Vegetative growth of young apple trees trained in vertical axis were studied with ' Red Fuji', 'Jonagold', 'Orin' and ' Starkrimson' on M7, MM106, M26 interstocks in northern China. About 30 branches sprouted from the central leader of the trees during the 4 years after planting for ' Red Fuji' and 'Jonagold', and 26.7 and 20 branches respectively for 'Orin' and 'Starkrimson'. Moreover the 2-year-old section of the central leader had the strongest capacity to sprout new branches (and sometimes the 1-year-old section too), and sprouted more new shoots than the other section. The total new shoots including spurs on the 4-year-old trees reached 631 per tree for 'Jonagold', about 480 for 'Red Fuji' and 'Orin', and 312 for 'Starkrimson'. Percentage of spurs was about 61% for 'Red Fuji', 73% for 'Jonagold' and 'Orin', and 81% for 'Starkrimson'. Growth vigor of the central leader and limbs of the young apple trees could quickly decline: the growth of the central leader decreased markedly in the fourth year after planting, and branches from the central leader grew vigorously only in the current growth season or in the first two years after branching.展开更多
The WRKY transcription factors play important roles in plant growth and resistance,but only a few members have been identified in strawberry.Here we identified a WRKY transcription factor,FvWRKY50,in diploid strawberr...The WRKY transcription factors play important roles in plant growth and resistance,but only a few members have been identified in strawberry.Here we identified a WRKY transcription factor,FvWRKY50,in diploid strawberry which played essential roles in strawberry vegetative growth,and reproductive growth.Knocking out FvWRKY50 by genome editing accelerated flowering time and leaf senescence but delayed anthocyanin accumulation in fruit.Further analysis showed that FvWRKY50 acted as a transcriptional repressor to negatively regulate the expression of flowering-and leaf senescence-related genes,including FvFT2,FvCO,FvFT3,and FvSAUR36.Notably,FvWRKY50 directly upregulated the expression of FvCHI and FvDFR by binding their promoter under normal conditions,but at low temperature FvWRKY50 was phosphorylated by FvMAPK3 and then induced protein degradation by ubiquitination,delaying anthocyanin accumulation.In addition,the homozygous mutant of FvWRKY50 was smaller while the biallelic mutant showed normal size.These new findings provide important clues for us to further reveal the regulatory mechanisms of strawberry growth and fruit ripening.展开更多
The study was conducted in Zhifanggou Watershed,Shaanxi Province,China,to evaluate the effect of different vegetation types on hot-water extractable C,N and P fractions,with the aim to determine whether hot-water extr...The study was conducted in Zhifanggou Watershed,Shaanxi Province,China,to evaluate the effect of different vegetation types on hot-water extractable C,N and P fractions,with the aim to determine whether hot-water extractable fractions could be used as indicators of soil quality change in Loess Plateau.The six vegetation types established in 1975 were（i） Robinia pseudoacacia L.,（ii） Caragana korshinkii Kom.,（iii） Pinus tabulaeformis Carr.,（iv） P.tabulaeformis-Amorpha fruticosa L.,（v） R.pseudoacacia-A.fruticosa,and（vi） grassland.A cropped hillslope plot and a Platycladus orientalis L.native forest plot were used as references.The results indicated that the conversion of native forest to cropland resulted in a significant decline in the hot-water extractable C,N and P fractions.Hot-water extractable C,N,and P increased when cultivated land was revegetated,but after 30 years the amount of hot-water extractable C,N,and P in revegetated fields was still much lower compared to native forest.Hot-water extractable fractions increased more under mixed-forest than under pure-forest stands.Furthermore,there was a significant correlation between the hot-water extractable fractions and soil chemical and microbiological properties.The results showed that hot-water extractable fractions could be used as indicators of soil quality change on the Loess Plateau.展开更多
BACKGROUND Persistent vegetative state(PVS)is a devastating and long-lasting clinical condition with high morbidity and mortality;currently,there are no available effective interventions.CASE SUMMARY We report the cas...BACKGROUND Persistent vegetative state(PVS)is a devastating and long-lasting clinical condition with high morbidity and mortality;currently,there are no available effective interventions.CASE SUMMARY We report the case of an 11-year-old boy with PVS caused by severe intracerebral bleeding in the left hemisphere following anticoagulation treatment.The patient’s PVS severity showed no notable improvement after 2-mo neuroprotective treatment and rehabilitation,including nerve growth factor and baclofen,hyperbaric oxygen,and comprehensive bedside rehabilitation therapies.Daily inhalation treatment(4-6 h)of high-concentration hydrogen(H2)gas(66.6%H2+33.3%O2)was provided.Surprisingly,the patient’s orientation,consciousness,ability to speak,facial expressions,and locomotor function were significantly restored,along with improvements in essential general health status,after H2 gas inhalation treatment,which was consistent with stabilized neuropathology in the left hemisphere and increased Hounsfield unit values of computed tomography in the right hemisphere.The patient finally recovered to a near normal conscious state with a Coma Recovery Scale-Revised Score of 22 from his previous score of 3.CONCLUSION Phase 1 clinical trials are needed to explore the safety and efficacy of H2 gas inhalation in patients with PVS.展开更多
文摘Elaeagnaceae Juss. belongs to the family Elaeagnus L. and Shepherdia Nutt. This study aims at the significance and vegetative reproduction of species of the family (Elaeagnus macrophylla Thunb. and Shepherdia canadensis (L.) Nutt.) in the national economy. The trials were carried out in the experimental site of SamSU’s Botanic Garden, which is named after Sharof Rashidov. The study’s findings are based on the results obtained by planting cuttings of S. canadensis and E. macrophylla species of varying sizes and durations, as well as the cuttings’ roots and the impacts of growth chemicals.
基金supported by the National Natural Science Foundation of China(5201101621)。
文摘Leaves are the main organ for photosynthesis and organic synthesis in cotton.Leaf shape has important effects on photosynthetic efficiency and canopy formation,thereby affecting cotton yield.Previous studies have shown that LMI1(LATE MERISTEM IDENTITY1)is the main gene regulating leaf shape.In this study,the LMI1 gene was inserted into the 35S promoter expression vector,and cotton plants overexpressing LMI1(OE)were obtained through genetic transformation.Statistical analysis of the biological traits of the T_(1) and T_(2) populations showed that compared to the wild type(WT),OE plants had significantly larger leaves,thicker stems and significantly greater dry weight.Furthermore,plant sections of the main vein and petiole showed that the numbers of cells in those tissues of OE plants were significantly greater.In addition,RNA-seq analysis revealed the differential expression of genes related to gibberellin synthesis and NAC gene family(genes containing the NAC domain)between the OE and WT plants,suggesting that LMI1 is involved in secondary wall formation and cell proliferation,which promotes stem thickening.Moreover,Gene Ontology(GO)analysis revealed enrichment in the terms of calcium ion binding,and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis showed enrichment in the terms of fatty acid degradation,phosphatidylinositol signal transduction system,and c AMP(cyclic adenosine monophosphate)signal pathway.These results suggested that LMI1 OE plants are responsive to gibberellin hormone signals,and have altered messenger signals(c AMP,Ca^(2+))which amplify this function,to promote stronger aboveground vegetative growth.This study found the LMI1 greatly increased the vegetative growth in cotton,which is the basic requirement for higher yield.
文摘The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. Endogenous IAA, DHZ+DHZR, iPA increased significantly during the floral bud formation, while Z+ZR remained changed. The levels of cytokinins, DHZ+DHZR, iPA, and Z+ZR all increased significantly during the vegetative bud formation, however IAA level was reduced during the first 7 days of culture and increased to two thirds of initial values on the day when the bud primordia were formed. The results suggested that the initiation of floral buds was associated with a high IAA/CTK ratio, whereas the induction of vegetative bud differentiation was related to a low IAA/CTK ratio.
文摘The ultrastructure of the vegetative cells of Nostoc flagelliforme Born. et Flah. was investigated with high pressure freezing and freeze substitution technique and compared with the results obtained by using conventional preparation methods. During the processes of chemical fixation, dehydration and embedding, the cell structures might be more artificially modified than that obtained from high pressure freezing and freeze substitution. With the present method, the sheath of N. flagelliforme could be well penetrated and no extra big space could exist between the cell and the sheath. The cell protoplasm rarely shrinked. Some fine structures of cell inclusions and unit membranes became visualized. Many bacteria were harbored in the sheath. In addition, the presence of big vacuoles in the cell of N. flagelliforme as well as the presence of bacteria in the sheath shown in the present preparation for cyanobacteria has not been described so far in the literature.
文摘[Objective] This study aimed to identify possible quantitative trait loci (QTL) for vegetative drought tolerance traits of rice (Oryza sativa L.). [Method] A total of 184 rice genotypes were field screened and 156 SSR markers randomly distributed at each 3 Mb bin were used through genome-wide scanning method and association analysis to detect QTLs for vegetative drought tolerance traits leaf rolling (LER), leaf drying (LED) and drought recovery rate (DRR). [Result] The experimental rice genotypes showed varied response under vegetative drought, LER, LED and DRR were highly correlated. Population structure was detected at K=3 and K=7, certain extent of admixture existed in the experimental rice genotypes, relative kinship of the rice genotypes ranged from 0 to 0.924 5. Significant linkage disequilibrium among SSR markers was detected. Sixteen SSR markers have been detected to be associated with vegetative drought tolerance traits, four for LER, eight for LED and four for DDR. Most of the markers were associated with more than one trait, indicating a single mechanism might involve in expression of several related traits, i.e. osmotic adjustment. RM107 (Chr.9) was associated with all the three traits and fell exactly within or closely nearby to previous reported regions, was a major QTL for vegetative drought tolerance, RM477 (Chr.8) was significantly associated with DRR and extremely significantly associated with LER was probably another major QTL for vegetative drought tolerance. [Conclusion] Association mapping is a very effective method for describing complex traits like drought tolerance.
文摘Structural and organizational changes of the vegetative cell in Amaryllis vittata Ait. during such dynamic processes of pollen as hydration, activation and germination have been examined with electron microscopy. The mature pollen grain is composed of such organelles as plastids, mitochondria,endoplasmic reticulum, dictyosomes and lipid bodies which are in their resting state. Microfilaments appear as aggregates. After pollen activation, however,the organelles undergo great changes in number and shape: the lamellae of plastids and the cristae of mitochondria increase conspicuously in number, the cisternae of the endoplasmic reticulum become narrower; the dictyosomes produce vesicles actively the lipid bodies become degraded and the microfilament aggregates disperse. Cortical microtubules and spiny vesicles appear in the cytoplasm after germination of the pollen tube. No apparent structural changes of the organelles were noticed any longer during this period and microfilaments are distributed throughout the entire pollen tube as a three-dimensional network.
文摘Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid （IAA） @ 2000, 4000 （ppm）, indolebutyric acid （IBA） @ 2000, 4000 （ppm） and naphthlcetic acid （NAA） @ 2000, 4000 （ppm） in dry formulation in the Forest Nursery, Faculty of Forestry, SKUAST-K, Shalimar. The cuttings treated with IBA @ 4000 ppm and IBA @ 2000 ppm had a sprouting rate of 75% and 50%, respectively, which was significantly higher than that of control and other treatments. The highest rooting rate （50%） was recorded in the cuttings with the application of IBA @ 4000 ppm. The cuttings treated with IBA @ 2000 ppm had 25% rooting rate. All other treatments along with control （talc powder） failed to induce rooting. It was concluded that IBA @ 4000 ppm was a better-applied concentration for vegetative propagation ofA. indica under Kashmir conditions.
文摘Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid(IAA) @ 2000, 4000(ppm), indolebutyric acid(IBA) @ 2000, 4000(ppm) and naphthlcetic acid(NAA) @ 2000, 4000(ppm) in dry formulation in the Forest Nursery, Faculty of Forestry, SKUAST-K, Shalimar.The cuttings treated with IBA @ 4000 ppm and IBA @ 2000 ppm had a sprouting rate of 75% and 50%, respectively, which was significantly higher than that of control and other treatments.The highest rooting rate(50%) was recorded in the cuttings with the application of IBA @ 4000 ppm.The cuttings treated with IBA @ 2000 ppm had 25% rooting rate.All other treatments along with control(talc powder) failed to induce rooting.It was concluded that IBA @ 4000 ppm was a better-applied concentration for vegetative propagation of A.indica under Kashmir conditions.
文摘Vegetative filter strip (VFS) is a main kind of Best Management Practices for the control of non-point source pollution. The goal of this paper is to evaluate the effectiveness of VFS in Chinese northwest regions. Three VFSs with natural grass and Hippophae rhamnoides/grass patterns have been constructed in the bank slope of Xiaohuashan reservoir, Huaxian County, Shannxi Province. The removal effects of VFS and influencing factors have been analyzed based on field experiment data. The result reveals a positive effect on reducing the transportation of suspended solids, phosphorus and nitrogen in surface runoff, and it is more efficient on suspended solids removal. The experiment also shows that most of the suspended particles and pollutants bound to them were entrapped in the first 10 m of VFS. The main factors influencing effectiveness of VFS include vegetation patterns and inflow rate. In addition, inflow pollutant concentration has a larger impact on reducing total nitrogen and total phosphorus by VFS, but the reduction effect on SS has no significant difference.
文摘The ultrastructural characteristics and the subcellular localization of vegetative storage proteins(VSPs)of Ginkgo biloba L.were systematically studied under the electron microscope.Results indicated that the VSPs of G.biloba were mostly distributed in the small vacuoles of the phloem parenchyma cells.The VSPs of phloem parenchyma cells were produced in cytoplasm,then separated by the inflated cisternae of endoplasmic reticulum(ER),plasmalemma invagination or Golgi body vesicles,resulting in the formation of vacuole filled with proteins.Three kinds of VSPs were detected:granular,floccular and massive VSPs,which were distributed in different tissue cells and different cells of the same tissue or vacuoles of the same cells.VSPs accumulated in autumn and kept at high level throughout the winter.In the following spring,the bud started growing and VSPs were completely mobilized.With new shoots growth,VSPs resumed accumulating in late summer and early autumn.
文摘For the first time, the anatomical structure of the vegetative organs of the species J. hippolyti and J. narbuti was studied in the conditions of Kyzylkum (Uzbekistan, Province Bukhara) and revealed the diagnostic features of these species. A different combination of xeromorphic and mesomorphic features was determined, which ensures adaptation to habitat conditions. In J. narbuti xeromorphic features predominate, which indicates a more adaptable and wide distribution in the natural habitat of this species. In J. hippolyti—mesomorphic signs that show the rarity of the species in their natural habitat, compared to J. narbuti.
基金supported by the National Natural Science Foundation of China(81671198)the Grant of Shanghai Municipal Education Commission-Gaofeng Clinical Medicine(20152212)the Shanghai Shenkang Clinical Research Plan of the Shanghai Hospital Development Center(16CR3011A)
文摘The vegetative state is a complex condition with unclear mechanisms and limited diagnostic, prognostic, and therapeutic methods. In this study, we aimed to explore the proteomic profile of tears from patients in a traumatic vegetative state and identify potential diagnostic markers using tears-a body fluid that can be collected noninvasively. Using iTRAQ quantitative proteomic technology, in the discovery phase, tear samples collected from 16 patients in a traumatic vegetative state and 16 normal individuals were analyzed. Among 1080 identified tear proteins, 57 were upregulated and 15 were downregulated in the patients compared to the controls. Bioinformatics analysis revealed that the differentially-expressed proteins were mainly involved in the wound response and immune response signaling pathways. Furthermore, we verified the levels of 7 differentially-expressed proteins in tears from 50 traumatic vegetative state patients and 50 normal controls （including the samples used in the discovery phase） using ELISA. The results showed that this 7-protein panel had a high discrimination ability for traumatic vegetative state （area under the curve = 0.999）. In sum- mary, the altered tear proteomic profile identified in this study provides a basis for potential tear protein markers for diagnosis and prognosis of the traumatic vegetative state and also provides novel insights into the mechanisms of traumatic vegetative state.
基金funded by Nation Key Technology R&D Program during the 11th Five-Year Plan Period(2008BADB3- B01)Forage Germplasm Resources Protection Project(Maintenance,Reproduction and Storage of Temperate Backup Library of Forage,2008-11)Crop Germplasm Conservation and Utilization Project of the Ministry of Agriculture (NB08-2130135-43)
文摘The anatomical structures of roots, stems and leaves are very important indicators to＇evaluate drought resistance. The paper reports the relationship between anatomical structures of vegetative organs and drought resistance in forages.
基金supported by the the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD12B02)
文摘High temperature stress （HT） is efficient in breaking endo-dormancy of perennial trees. The effects of HT （50°C） on the respiration of dormant nectarine （Prunus persica var. nectariana cv. Shuguang） vegetative buds were evaluated in the research. We found that bud respiration was transiently inhibited by HT and the pentose phosphate pathway （PPP） and the cytochrome C pathway （CYT） were significantly affected. On the substrate level, PPP was activated in the HT-treated buds compared with the control group. However, the activation did mot occur until hours after HT treatment. The tricarboxylic acid cycle （TCA） in both the HT-treated buds and in the control group proceeded at a low level most of the time compared with total respiration. On the electron transfer level, CYT was transiently inhibited by HT but became significantly active in the later stage. CYT operation in the control group exhibited an attenuation process. The alternative pathway （ALT） fluctuated both in the HT-treated samples and in the control. The results suggest that the temporary CYT inhibition and the following PPP activation may be involved in HT-induced bud dormancy release and budburst mechanisms.
基金the National Natural Science Foundation of China(39970445)Program for Changjiang Scholars and Innovativc Rescarch Team in University(PCSIRT,IRT0453)+1 种基金 the Natural Science Foundation of Education Committee Scienceand Technology Committee in Sichuan Province of China.
文摘In this paper, the traits of immature embryo culture from 45 genotypes of bread wheat were investigated in detail, and trait correlation analysis in combination with histomorphological examination was used to study the cytological origin of flower as well as the correlation between it and vegetative shoot development from the calli. The rate of floral development in the subculture was highly significantly and positively related to that of the differentiation culture, which reveals that the genetic system of floral development from immature embryo-derived calli in wheat was highly correspondent and stable. The rate of floral development was related neither to the traits in the dedifferentiation culture, nor to the vegetative shoot formation from the total induced calli, but significantly and positively related to that of the calli without leaf-like green spots in the differentiation culture. These findings indicate the partial correlation as well as differentiation between flower and vegetative shoot development. The floral development might only be associated with the genetic system controlling organ regeneration, while vegetative shoots formed from the two ways, including somatic embryogenesis and organ regeneration.
文摘Vegetative growth of young apple trees trained in vertical axis were studied with ' Red Fuji', 'Jonagold', 'Orin' and ' Starkrimson' on M7, MM106, M26 interstocks in northern China. About 30 branches sprouted from the central leader of the trees during the 4 years after planting for ' Red Fuji' and 'Jonagold', and 26.7 and 20 branches respectively for 'Orin' and 'Starkrimson'. Moreover the 2-year-old section of the central leader had the strongest capacity to sprout new branches (and sometimes the 1-year-old section too), and sprouted more new shoots than the other section. The total new shoots including spurs on the 4-year-old trees reached 631 per tree for 'Jonagold', about 480 for 'Red Fuji' and 'Orin', and 312 for 'Starkrimson'. Percentage of spurs was about 61% for 'Red Fuji', 73% for 'Jonagold' and 'Orin', and 81% for 'Starkrimson'. Growth vigor of the central leader and limbs of the young apple trees could quickly decline: the growth of the central leader decreased markedly in the fourth year after planting, and branches from the central leader grew vigorously only in the current growth season or in the first two years after branching.
基金Thisworkwas supported by the National Natural Science Foundation of China(32222074,32072551)the National Key Research and Development Program of China(2022YFD2100102-3)+2 种基金111 Project(B17043)Beijing Innovation Consortium of Agriculture Research System(BAIC04-2022)the 2115 Talent Development Program of China Agricultural University.
文摘The WRKY transcription factors play important roles in plant growth and resistance,but only a few members have been identified in strawberry.Here we identified a WRKY transcription factor,FvWRKY50,in diploid strawberry which played essential roles in strawberry vegetative growth,and reproductive growth.Knocking out FvWRKY50 by genome editing accelerated flowering time and leaf senescence but delayed anthocyanin accumulation in fruit.Further analysis showed that FvWRKY50 acted as a transcriptional repressor to negatively regulate the expression of flowering-and leaf senescence-related genes,including FvFT2,FvCO,FvFT3,and FvSAUR36.Notably,FvWRKY50 directly upregulated the expression of FvCHI and FvDFR by binding their promoter under normal conditions,but at low temperature FvWRKY50 was phosphorylated by FvMAPK3 and then induced protein degradation by ubiquitination,delaying anthocyanin accumulation.In addition,the homozygous mutant of FvWRKY50 was smaller while the biallelic mutant showed normal size.These new findings provide important clues for us to further reveal the regulatory mechanisms of strawberry growth and fruit ripening.
基金financially supported by the Strategic Technology Project of Chinese Academy of Sciences,China(XDA05060300)the Science and Technology Research and Development Program of Shaanxi Province,China(2011KJXX63)the Fundamental Research Funds for the Central Universities,China(ZD2013021)
文摘The study was conducted in Zhifanggou Watershed,Shaanxi Province,China,to evaluate the effect of different vegetation types on hot-water extractable C,N and P fractions,with the aim to determine whether hot-water extractable fractions could be used as indicators of soil quality change in Loess Plateau.The six vegetation types established in 1975 were（i） Robinia pseudoacacia L.,（ii） Caragana korshinkii Kom.,（iii） Pinus tabulaeformis Carr.,（iv） P.tabulaeformis-Amorpha fruticosa L.,（v） R.pseudoacacia-A.fruticosa,and（vi） grassland.A cropped hillslope plot and a Platycladus orientalis L.native forest plot were used as references.The results indicated that the conversion of native forest to cropland resulted in a significant decline in the hot-water extractable C,N and P fractions.Hot-water extractable C,N,and P increased when cultivated land was revegetated,but after 30 years the amount of hot-water extractable C,N,and P in revegetated fields was still much lower compared to native forest.Hot-water extractable fractions increased more under mixed-forest than under pure-forest stands.Furthermore,there was a significant correlation between the hot-water extractable fractions and soil chemical and microbiological properties.The results showed that hot-water extractable fractions could be used as indicators of soil quality change on the Loess Plateau.
文摘BACKGROUND Persistent vegetative state(PVS)is a devastating and long-lasting clinical condition with high morbidity and mortality;currently,there are no available effective interventions.CASE SUMMARY We report the case of an 11-year-old boy with PVS caused by severe intracerebral bleeding in the left hemisphere following anticoagulation treatment.The patient’s PVS severity showed no notable improvement after 2-mo neuroprotective treatment and rehabilitation,including nerve growth factor and baclofen,hyperbaric oxygen,and comprehensive bedside rehabilitation therapies.Daily inhalation treatment(4-6 h)of high-concentration hydrogen(H2)gas(66.6%H2+33.3%O2)was provided.Surprisingly,the patient’s orientation,consciousness,ability to speak,facial expressions,and locomotor function were significantly restored,along with improvements in essential general health status,after H2 gas inhalation treatment,which was consistent with stabilized neuropathology in the left hemisphere and increased Hounsfield unit values of computed tomography in the right hemisphere.The patient finally recovered to a near normal conscious state with a Coma Recovery Scale-Revised Score of 22 from his previous score of 3.CONCLUSION Phase 1 clinical trials are needed to explore the safety and efficacy of H2 gas inhalation in patients with PVS.
