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Joint Estimation of SOH and RUL for Lithium-Ion Batteries Based on Improved Twin Support Vector Machineh 被引量:1
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作者 Liyao Yang Hongyan Ma +1 位作者 Yingda Zhang Wei He 《Energy Engineering》 EI 2025年第1期243-264,共22页
Accurately estimating the State of Health(SOH)and Remaining Useful Life(RUL)of lithium-ion batteries(LIBs)is crucial for the continuous and stable operation of battery management systems.However,due to the complex int... Accurately estimating the State of Health(SOH)and Remaining Useful Life(RUL)of lithium-ion batteries(LIBs)is crucial for the continuous and stable operation of battery management systems.However,due to the complex internal chemical systems of LIBs and the nonlinear degradation of their performance,direct measurement of SOH and RUL is challenging.To address these issues,the Twin Support Vector Machine(TWSVM)method is proposed to predict SOH and RUL.Initially,the constant current charging time of the lithium battery is extracted as a health indicator(HI),decomposed using Variational Modal Decomposition(VMD),and feature correlations are computed using Importance of Random Forest Features(RF)to maximize the extraction of critical factors influencing battery performance degradation.Furthermore,to enhance the global search capability of the Convolution Optimization Algorithm(COA),improvements are made using Good Point Set theory and the Differential Evolution method.The Improved Convolution Optimization Algorithm(ICOA)is employed to optimize TWSVM parameters for constructing SOH and RUL prediction models.Finally,the proposed models are validated using NASA and CALCE lithium-ion battery datasets.Experimental results demonstrate that the proposed models achieve an RMSE not exceeding 0.007 and an MAPE not exceeding 0.0082 for SOH and RUL prediction,with a relative error in RUL prediction within the range of[-1.8%,2%].Compared to other models,the proposed model not only exhibits superior fitting capability but also demonstrates robust performance. 展开更多
关键词 State of health remaining useful life variational modal decomposition random forest twin support vector machine convolutional optimization algorithm
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Tip-enhanced Raman scattering of glucose molecules 被引量:3
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作者 Zhonglin Xie Chao Meng +3 位作者 Donghua Yue Lei Xu Ting Mei Wending Zhang 《Opto-Electronic Science》 2025年第5期2-9,共8页
Glucose molecules are of great significance being one of the most important molecules in metabolic chain.However,due to the small Raman scattering cross-section and weak/non-adsorption on bare metals,accurately obtain... Glucose molecules are of great significance being one of the most important molecules in metabolic chain.However,due to the small Raman scattering cross-section and weak/non-adsorption on bare metals,accurately obtaining their"fingerprint information"remains a huge obstacle.Herein,we developed a tip-enhanced Raman scattering(TERS)technique to address this challenge.Adopting an optical fiber radial vector mode internally illuminates the plasmonic fiber tip to effectively suppress the background noise while generating a strong electric-field enhanced tip hotspot.Furthermore,the tip hotspot approaching the glucose molecules was manipulated via the shear-force feedback to provide more freedom for selecting substrates.Consequently,our TERS technique achieves the visualization of all Raman modes of glucose molecules within spectral window of 400-3200 cm^(-1),which is not achievable through the far-field/surface-enhanced Raman,or the existing TERS techniques.Our TERS technique offers a powerful tool for accurately identifying Raman scattering of molecules,paving the way for biomolecular analysis. 展开更多
关键词 tip-enhanced Raman scattering scanning near-field optical microscope fiber vector light field tip nanofocusing light source
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Machine learning of pyrite geochemistry reconstructs the multi-stage history of mineral deposits 被引量:1
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作者 Pengpeng Yu Yuan Liu +5 位作者 Hanyu Wang Xi Chen Yi Zheng Wei Cao Yiqu Xiong Hongxiang Shan 《Geoscience Frontiers》 2025年第3期81-93,共13页
The application of machine learning for pyrite discrimination establishes a robust foundation for constructing the ore-forming history of multi-stage deposits;however,published models face challenges related to limite... The application of machine learning for pyrite discrimination establishes a robust foundation for constructing the ore-forming history of multi-stage deposits;however,published models face challenges related to limited,imbalanced datasets and oversampling.In this study,the dataset was expanded to approximately 500 samples for each type,including 508 sedimentary,573 orogenic gold,548 sedimentary exhalative(SEDEX)deposits,and 364 volcanogenic massive sulfides(VMS)pyrites,utilizing random forest(RF)and support vector machine(SVM)methodologies to enhance the reliability of the classifier models.The RF classifier achieved an overall accuracy of 99.8%,and the SVM classifier attained an overall accuracy of 100%.The model was evaluated by a five-fold cross-validation approach with 93.8%accuracy for the RF and 94.9%for the SVM classifier.These results demonstrate the strong feasibility of pyrite classification,supported by a relatively large,balanced dataset and high accuracy rates.The classifier was employed to reveal the genesis of the controversial Keketale Pb-Zn deposit in NW China,which has been inconclusive among SEDEX,VMS,or a SEDEX-VMS transition.Petrographic investigations indicated that the deposit comprises early fine-grained layered pyrite(Py1)and late recrystallized pyrite(Py2).The majority voting classified Py1 as the VMS type,with an accuracy of RF and SVM being 72.2%and 75%,respectively,and confirmed Py2 as an orogenic type with 74.3% and 77.1%accuracy,respectively.The new findings indicated that the Keketale deposit originated from a submarine VMS mineralization system,followed by late orogenic-type overprinting of metamorphism and deformation,which is consistent with the geological and geochemical observations.This study further emphasizes the advantages of Machine learning(ML)methods in accurately and directly discriminating the deposit types and reconstructing the formation history of multi-stage deposits. 展开更多
关键词 Machine learning Random forest Support vector machine PYRITE Multi-stage genesis Keketale deposit
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Comprehensive analysis of noise in Macao Science Satellite-1 vector magnetometer data 被引量:1
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作者 SiShan Song Fan Yin +4 位作者 Qin Yan Hermann Lühr Chao Xiong Yi Jiang PengFei Liu 《Earth and Planetary Physics》 2025年第3期532-540,共9页
The Macao Science Satellite-1(known as MSS-1)is the first scientific exploration satellite that was designed to measure the Earth's low latitude magnetic field at high resolution and with high precision by collect... The Macao Science Satellite-1(known as MSS-1)is the first scientific exploration satellite that was designed to measure the Earth's low latitude magnetic field at high resolution and with high precision by collecting data in a near-equatorial orbit.Magnetic field data from MSS-1's onboard Vector Fluxgate Magnetometer(VFM),collected at a sample rate of 50 Hz,allows us to detect and investigate sources of magnetic data contamination,from DC to relevant Nyquist frequency.Here we report two types of artificial disturbances in the VFM data.One is V-shaped events concentrated at night,with frequencies sweeping from the Nyquist frequency down to zero and back up.The other is 5-Hz events(ones that exhibit a distinct 5 Hz spectrum peak);these events are always accompanied by intervals of spiky signals,and are clearly related to the attitude control of the satellite.Our analyses show that VFM noise levels in daytime are systematically lower than in nighttime.The daily average noise levels exhibit a period of about 52 days.The V-shaped events are strongly correlated with higher VFM noise levels. 展开更多
关键词 Macao Science Satellite-1 Vector Fluxgate Magnetometer artificial disturbances noise features
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Optimization method of conditioning factors selection and combination for landslide susceptibility prediction 被引量:1
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作者 Faming Huang Keji Liu +4 位作者 Shuihua Jiang Filippo Catani Weiping Liu Xuanmei Fan Jinsong Huang 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第2期722-746,共25页
Landslide susceptibility prediction(LSP)is significantly affected by the uncertainty issue of landslide related conditioning factor selection.However,most of literature only performs comparative studies on a certain c... Landslide susceptibility prediction(LSP)is significantly affected by the uncertainty issue of landslide related conditioning factor selection.However,most of literature only performs comparative studies on a certain conditioning factor selection method rather than systematically study this uncertainty issue.Targeted,this study aims to systematically explore the influence rules of various commonly used conditioning factor selection methods on LSP,and on this basis to innovatively propose a principle with universal application for optimal selection of conditioning factors.An'yuan County in southern China is taken as example considering 431 landslides and 29 types of conditioning factors.Five commonly used factor selection methods,namely,the correlation analysis(CA),linear regression(LR),principal component analysis(PCA),rough set(RS)and artificial neural network(ANN),are applied to select the optimal factor combinations from the original 29 conditioning factors.The factor selection results are then used as inputs of four types of common machine learning models to construct 20 types of combined models,such as CA-multilayer perceptron,CA-random forest.Additionally,multifactor-based multilayer perceptron random forest models that selecting conditioning factors based on the proposed principle of“accurate data,rich types,clear significance,feasible operation and avoiding duplication”are constructed for comparisons.Finally,the LSP uncertainties are evaluated by the accuracy,susceptibility index distribution,etc.Results show that:(1)multifactor-based models have generally higher LSP performance and lower uncertainties than those of factors selection-based models;(2)Influence degree of different machine learning on LSP accuracy is greater than that of different factor selection methods.Conclusively,the above commonly used conditioning factor selection methods are not ideal for improving LSP performance and may complicate the LSP processes.In contrast,a satisfied combination of conditioning factors can be constructed according to the proposed principle. 展开更多
关键词 Landslide susceptibility prediction Conditioning factors selection Support vector machine Random forest Rough set Artificial neural network
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A Support Vector Machine(SVM)Model for Privacy Recommending Data Processing Model(PRDPM)in Internet of Vehicles
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作者 Ali Alqarni 《Computers, Materials & Continua》 SCIE EI 2025年第1期389-406,共18页
Open networks and heterogeneous services in the Internet of Vehicles(IoV)can lead to security and privacy challenges.One key requirement for such systems is the preservation of user privacy,ensuring a seamless experie... Open networks and heterogeneous services in the Internet of Vehicles(IoV)can lead to security and privacy challenges.One key requirement for such systems is the preservation of user privacy,ensuring a seamless experience in driving,navigation,and communication.These privacy needs are influenced by various factors,such as data collected at different intervals,trip durations,and user interactions.To address this,the paper proposes a Support Vector Machine(SVM)model designed to process large amounts of aggregated data and recommend privacy preserving measures.The model analyzes data based on user demands and interactions with service providers or neighboring infrastructure.It aims to minimize privacy risks while ensuring service continuity and sustainability.The SVMmodel helps validate the system’s reliability by creating a hyperplane that distinguishes between maximum and minimum privacy recommendations.The results demonstrate the effectiveness of the proposed SVM model in enhancing both privacy and service performance. 展开更多
关键词 Support vector machine big data IoV PRIVACY-PRESERVING
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Machine Learning Techniques in Predicting Hot Deformation Behavior of Metallic Materials
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作者 Petr Opela Josef Walek Jaromír Kopecek 《Computer Modeling in Engineering & Sciences》 SCIE EI 2025年第1期713-732,共20页
In engineering practice,it is often necessary to determine functional relationships between dependent and independent variables.These relationships can be highly nonlinear,and classical regression approaches cannot al... In engineering practice,it is often necessary to determine functional relationships between dependent and independent variables.These relationships can be highly nonlinear,and classical regression approaches cannot always provide sufficiently reliable solutions.Nevertheless,Machine Learning(ML)techniques,which offer advanced regression tools to address complicated engineering issues,have been developed and widely explored.This study investigates the selected ML techniques to evaluate their suitability for application in the hot deformation behavior of metallic materials.The ML-based regression methods of Artificial Neural Networks(ANNs),Support Vector Machine(SVM),Decision Tree Regression(DTR),and Gaussian Process Regression(GPR)are applied to mathematically describe hot flow stress curve datasets acquired experimentally for a medium-carbon steel.Although the GPR method has not been used for such a regression task before,the results showed that its performance is the most favorable and practically unrivaled;neither the ANN method nor the other studied ML techniques provide such precise results of the solved regression analysis. 展开更多
关键词 Machine learning Gaussian process regression artificial neural networks support vector machine hot deformation behavior
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A Survey on the Existence of Harmonic Metrics on Vector Bundles
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作者 SHEN Zhenghan ZHANG Xi 《数学进展》 北大核心 2025年第2期390-404,共15页
In this paper,we give a survey on the existence of Hermitian-Einstein metrics and harmonic metrics.
关键词 Hermitian-Einstein metric harmonic metric holomorphic vector bundle non-Hermitian Yang-Mills bundle
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Thrust-vectoring schemes for electric propulsion systems:A review
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作者 Andrei SHUMEIKO Victor TELEKH Sergei RYZHKOV 《Chinese Journal of Aeronautics》 2025年第6期179-203,共25页
Thrust-vectoring capability has become a critical feature for propulsion systems as space missions move from static to dynamic.Thrust-vectoring is a well-developed area of rocket engine science.For electric propulsion... Thrust-vectoring capability has become a critical feature for propulsion systems as space missions move from static to dynamic.Thrust-vectoring is a well-developed area of rocket engine science.For electric propulsion,however,it is an evolving field that has taken a new leap forward in recent years.A review and analysis of thrust-vectoring schemes for electric propulsion systems have been conducted.The scope of this review includes thrust-vectoring schemes that can be implemented for electrostatic,electromagnetic,and beam-driven thrusters.A classification of electric propulsion schemes that provide thrust-vectoring capability is developed.More attention is given to schemes implemented in laboratory prototypes and flight models.The final part is devoted to a discussion on the suitability of different electric propulsion systems with thrust-vectoring capability for modern space mission operations.The thrust-vectoring capability of electric propulsion is necessary for inner and outer space satellites,which are at a disadvantage with conventional unidirectional propulsion systems due to their limited maneuverability. 展开更多
关键词 Electric propulsion Spacecraft propulsion Plasma sources Flight control systems Thrust vectoring Thrust vector control
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A Binary Vulnerability Similarity Detection Model Based on Deep Graph Matching
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作者 Yangzhi Zhang 《Journal of Electronic Research and Application》 2025年第5期291-298,共8页
To enhance network security,this study employs a deep graph matching model for vulnerability similarity detection.The model utilizes a Word Embedding layer to vectorize data words,an Image Embedding layer to vectorize... To enhance network security,this study employs a deep graph matching model for vulnerability similarity detection.The model utilizes a Word Embedding layer to vectorize data words,an Image Embedding layer to vectorize data graphs,and an LSTM layer to extract the associations between word and graph vectors.A Dropout layer is applied to randomly deactivate neurons in the LSTM layer,while a Softmax layer maps the LSTM analysis results.Finally,a fully connected layer outputs the detection results with a dimension of 1.Experimental results demonstrate that the AUC of the deep graph matching vulnerability similarity detection model is 0.9721,indicating good stability.The similarity scores for vulnerabilities such as memory leaks,buffer overflows,and targeted attacks are close to 1,showing significant similarity.In contrast,the similarity scores for vulnerabilities like out-of-bounds memory access and logical design flaws are less than 0.4,indicating good similarity detection performance.The model’s evaluation metrics are all above 97%,with high detection accuracy,which is beneficial for improving network security. 展开更多
关键词 Network security Word vectors Graph vector matrix Deep graph matching Vulnerability similarity
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Efficient rock joint detection from large-scale 3D point clouds using vectorization and parallel computing approaches
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作者 Yunfeng Ge Zihao Li +2 位作者 Huiming Tang Qian Chen Zhongxu Wen 《Geoscience Frontiers》 2025年第5期1-15,共15页
The application of three-dimensional(3D)point cloud parametric analyses on exposed rock surfaces,enabled by Light Detection and Ranging(LiDAR)technology,has gained significant popularity due to its efficiency and the ... The application of three-dimensional(3D)point cloud parametric analyses on exposed rock surfaces,enabled by Light Detection and Ranging(LiDAR)technology,has gained significant popularity due to its efficiency and the high quality of data it provides.However,as research extends to address more regional and complex geological challenges,the demand for algorithms that are both robust and highly efficient in processing large datasets continues to grow.This study proposes an advanced rock joint identification algorithm leveraging artificial neural networks(ANNs),incorporating parallel computing and vectorization of high-performance computing.The algorithm utilizes point cloud attributes—specifically point normal and point curvatures-as input parameters for ANNs,which classify data into rock joints and non-rock joints.Subsequently,individual rock joints are extracted using the density-based spatial clustering of applications with noise(DBSCAN)technique.Principal component analysis(PCA)is subsequently employed to calculate their orientations.By fully utilizing the computational power of parallel computing and vectorization,the algorithm increases the running speed by 3–4 times,enabling the processing of large-scale datasets within seconds.This breakthrough maximizes computational efficiency while maintaining high accuracy(compared with manual measurement,the deviation of the automatic measurement is within 2°),making it an effective solution for large-scale rock joint detection challenges.©2025 China University of Geosciences(Beijing)and Peking University. 展开更多
关键词 Rock joints Pointclouds Artificialneuralnetwork High-performance computing Parallel computing VECTORIZATION
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Evaluating vector winds over eastern China in 2022 predicted by the CMA-MESO model and ECMWF forecast
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作者 Fang Huang Mingjian Zeng +4 位作者 Zhongfeng Xu Boni Wang Ming Sun Hangcheng Ge Shoukang Wu 《Atmospheric and Oceanic Science Letters》 2025年第4期41-47,共7页
Vector winds play a crucial role in weather and climate,as well as the effective utilization of wind energy resources.However,limited research has been conducted on treating the wind field as a vector field in the eva... Vector winds play a crucial role in weather and climate,as well as the effective utilization of wind energy resources.However,limited research has been conducted on treating the wind field as a vector field in the evaluation of numerical weather prediction models.In this study,the authors treat vector winds as a whole by employing a vector field evaluation method,and evaluate the mesoscale model of the China Meteorological Administration(CMA-MESO)and ECMWF forecast,with reference to ERA5 reanalysis,in terms of multiple aspects of vector winds over eastern China in 2022.The results show that the ECMWF forecast is superior to CMA-MESO in predicting the spatial distribution and intensity of 10-m vector winds.Both models overestimate the wind speed in East China,and CMA-MESO overestimates the wind speed to a greater extent.The forecasting skill of the vector wind field in both models decreases with increasing lead time.The forecasting skill of CMA-MESO fluctuates more and decreases faster than that of the ECMWF forecast.There is a significant negative correlation between the model vector wind forecasting skill and terrain height.This study provides a scientific evaluation of the local application of vector wind forecasts of the CMA-MESO model and ECMWF forecast. 展开更多
关键词 Model evaluation Vector winds CMA-MESO ECMWF Forecasting skill
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Far-field calibration of automotive millimeter wave radar via near-field implementation
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作者 SUN Jinghu LIU Jiahuan +3 位作者 WEI Wenqiang YU Xianxiang CUI Guolong ZHANG Xiuyin 《Journal of Systems Engineering and Electronics》 2025年第3期694-700,共7页
To enhance direction of arrival(DOA)estimation accuracy,this paper proposes a low-cost method for calibrating farfield steering vectors of large aperture millimeter wave radar(mmWR).To this end,we first derive the ste... To enhance direction of arrival(DOA)estimation accuracy,this paper proposes a low-cost method for calibrating farfield steering vectors of large aperture millimeter wave radar(mmWR).To this end,we first derive the steering vectors with amplitude and phase errors,assuming that mmWR works in the time-sharing mode.Then,approximate relationship between the near-field calibration steering vector and the far-field calibration steering vector is analyzed,which is used to accomplish the mapping between the two of them.Finally,simulation results verify that the proposed method can effectively improve the angle measurement accuracy of mmWR with existing amplitude and phase errors. 展开更多
关键词 automotive millimeter wave radar far-field steering vector calibration near-field steering vector calibration direction of arrival(DOA)estimation low cost
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A VECTOR BUNDLE VALUED MIXED HARD LEFSCHETZ THEOREM
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作者 Zeng CHEN Guanxiang WANG 《Acta Mathematica Scientia》 2025年第2期514-524,共11页
In this paper,we obtain a vector bundle valued mixed hard Lefschetz theorem.The argument is mainly based on the works of Tien-Cuong Dinh and Viet-Anh Nguyen.
关键词 hard Lefschetz theorem holomorphic vector bundle Hermitian fat vector bundle
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Adeno-associated viral vectors for modeling Parkinson's disease in non-human primates
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作者 Julia Chocarro José L.Lanciego 《Neural Regeneration Research》 2026年第1期224-232,共9页
The development of clinical candidates that modify the natural progression of sporadic Parkinson's disease and related synucleinopathies is a praiseworthy endeavor,but extremely challenging.Therapeutic candidates ... The development of clinical candidates that modify the natural progression of sporadic Parkinson's disease and related synucleinopathies is a praiseworthy endeavor,but extremely challenging.Therapeutic candidates that were successful in preclinical Parkinson's disease animal models have repeatedly failed when tested in clinical trials.While these failures have many possible explanations,it is perhaps time to recognize that the problem lies with the animal models rather than the putative candidate.In other words,the lack of adequate animal models of Parkinson's disease currently represents the main barrier to preclinical identification of potential disease-modifying therapies likely to succeed in clinical trials.However,this barrier may be overcome by the recent introduction of novel generations of viral vectors coding for different forms of alpha-synuclein species and related genes.Although still facing several limitations,these models have managed to mimic the known neuropathological hallmarks of Parkinson's disease with unprecedented accuracy,delineating a more optimistic scenario for the near future. 展开更多
关键词 adeno-associated viral vectors ALPHA-SYNUCLEIN DOPAMINE Lewy bodies NEURODEGENERATION NEUROMELANIN NEUROPATHOLOGY substantia nigra
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Essential oil and furanosesquiterpenes from myrrh oleo-gum resin:a breakthrough in mosquito vector management
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作者 Eleonora Spinozzi Marta Ferrati +13 位作者 Cecilia Baldassarri Paolo Rossi Guido Favia Giorgio Cameli Giovanni Benelli Angelo Canale Livia De Fazi Roman Pavela Luana Quassinti Cristiano Giordani Fabrizio Araniti Loredana Cappellacci Riccardo Petrelli Filippo Maggi 《Natural Products and Bioprospecting》 2025年第2期51-67,共17页
Mosquitoes(Diptera:Culicidae)are vectors of various pathogens of public health concern,but replacing conventional insecticides remains a challenge.In this regard,natural products represent valuable sources of potentia... Mosquitoes(Diptera:Culicidae)are vectors of various pathogens of public health concern,but replacing conventional insecticides remains a challenge.In this regard,natural products represent valuable sources of potential insecticidal compounds,thus increasingly attracting research interest.Commiphora myrrha(T.Nees)Engl.(Burseraceae)is a medicinal plant whose oleo-gum resin is used in food,cosmetics,fragrances,and pharmaceuticals.Herein,the larvicidal potential of its essential oil(EO)was assessed on four mosquito species(Aedes albopictus Skuse,Ae.aegypti L.,Anopheles gambiae Giles and An.stephensi Liston),with LC_(50) values ranging from 4.42 to 16.80 μg/mL.The bio-guided EO fractionation identified furanosesquiterpenes as the main larvicidal compounds.A GC-MS-driven untargeted metabolomic analysis revealed 32 affected metabolic pathways in treated larvae.The EO non-target toxicity on Daphnia magna Straus(LC_(50)=4.51 μL/L)and its cytotoxicity on a human kidney cell line(HEK293)(IC50 of 14.38 μg/mL)were also assessed.This study shows the potential of plant products as innovative insecticidal agents and lays the ground-work for the possible exploitation of C.myrrha EO in the sustainable approaches for mosquito management. 展开更多
关键词 Arbovirus vector Commiphora myrrha Aedes aegypti Anopheles spp. BIOINSECTICIDE
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Development of an improved reverse genetics system for avian metapneumovirus(aMPV):A novel vaccine vector protects against aMPV and infectious bursal disease virus
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作者 Lingzhai Meng Yuntong Chen +14 位作者 Mengmeng Yu Peng Liu Xiaole Qi Xiaoxiao Xue Ru Guo Tao Zhang Mingxue Hu Wenrui Fan Ying Wang Suyan Wang Yanping Zhang Yongzhen Liu Yulu Duan Hongyu Cui Yulong Gao 《Journal of Integrative Agriculture》 2025年第5期1972-1986,共15页
Avian metapneumovirus(aMPV),a paramyxovirus,causes acute respiratory diseases in turkeys and swollen head syndrome in chickens.This study established a reverse genetics system for aMPV subtype B LN16-A strain based on... Avian metapneumovirus(aMPV),a paramyxovirus,causes acute respiratory diseases in turkeys and swollen head syndrome in chickens.This study established a reverse genetics system for aMPV subtype B LN16-A strain based on T7 RNA polymerase.Full-length cDNA of the LN16-A strain was constructed by assembling 5 cDNA fragments between the T7 promoter and hepatitis delta virus ribozyme.Transfection of this plasmid,along with the supporting plasmids encoding the N,P,M2-1,and L proteins of LN16-A into BSR-T7/5 cells,resulted in the recovery of aMPV subtype B.To identify an effective insertion site,the enhanced green fluorescent protein(EGFP)gene was inserted into different sites of the LN16-A genome to generate recombinant LN16-As.The results showed that the expression levels of EGFP at the site between the G and L genes of LN16-A were significantly higher than those at the other two sites(between the leader and N genes or replacing the SH gene).To verify the availability of the site between G and L for foreign gene expression,the VP2 gene of very virulent infectious bursal disease virus(vvIBDV)was inserted into this site,and recombinant LN16-A(rLN16A-vvVP2)was successfully rescued.Single immunization of specificpathogen-free chickens with rLN16A-vvVP2 induced high levels of neutralizing antibodies and provided 100%protection against the virulent aMPV subtype B and vvIBDV.Establishing a reverse genetics system here provides an important foundation for understanding aMPV pathogenesis and developing novel vector vaccines. 展开更多
关键词 avian metapneumovirus reverse genetics system insertion site PROTECTION vector vaccines
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A novel BaEVRless-LV packaging system for the production of lentiviral vectors for clinical-grade CAR-NK cell manufacturing
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作者 Yinyin Zhang Minghuan Zhang +5 位作者 Mengyuan Li Lihong Zong Qian Ye Wen Lei Wenhai Deng Wenbin Qian 《Cancer Biology & Medicine》 2025年第2期137-143,共7页
Chimeric antigen receptor natural killer(CAR-NK)cell therapy is an alternative immunotherapy that provides robust tumor-eliminating effects without inducing life-threatening toxicities and graft-versus-host disease.CA... Chimeric antigen receptor natural killer(CAR-NK)cell therapy is an alternative immunotherapy that provides robust tumor-eliminating effects without inducing life-threatening toxicities and graft-versus-host disease.CAR-NK cell therapy has enabled the development of“off-the-shelf”products that bypass the lengthy and expensive cell manufacturing process1. 展开更多
关键词 LENTIVIRAL packaging VECTORS alternative immunotherapy chimeric antigen receptor natural system cell manufacturing process NOVEL
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Seismic Prediction Methods for Tidal Flat Sand Bodies in the Shunbei Area of the Tarim Basin
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作者 Zhi-peng Sun Rui-zhao Yang +4 位作者 Jing-rui Chen Hao Zhang Shi-jie Zhang Peng-hui Yang Feng Geng 《Applied Geophysics》 2025年第1期176-196,235,共22页
The Tarim Basin has revealed numerous tight sandstone oil and gas reservoirs.The tidal fl at zone in the Shunbei area is currently in the detailed exploration stage,requiring a comprehensive description of the sand bo... The Tarim Basin has revealed numerous tight sandstone oil and gas reservoirs.The tidal fl at zone in the Shunbei area is currently in the detailed exploration stage,requiring a comprehensive description of the sand body distribution characteristics for rational exploration well deployment.However,using a single method for sand body prediction has yielded poor results.Seismic facies analysis can eff ectively predict the macro-development characteristics of sedimentary sand bodies but lacks the resolution to capture fine details.In contrast,single-well sedimentary facies analysis can describe detailed sand body development but struggles to reveal broader trends.Therefore,this study proposes a method that combines seismic facies analysis with single-well sedimentary microfacies analysis,using the lower section of the Kepingtage Formation in the Shunbei area as a case study.First,seismic facies were obtained through unsupervised vector quantization to control the macro-distribution characteristics of sand bodies,while principal component analysis(PCA)was applied to improve the depiction of fine sand body details from seismic attributes.Based on 3D seismic data,well-logging data,and geological interpretation results,a detailed structural interpretation was performed to establish a high-precision stratigraphic framework,thereby enhancing the accuracy of sand body prediction.Seismic facies analysis was then conducted to obtain the macro-distribution characteristics of the sand bodies.Subsequently,core data and logging curves from individual wells were used to clarify the vertical development characteristics of tidal channels and sandbars.Next,PCA was employed to select the seismic attributes most sensitive to sand bodies in diff erent sedimentary facies.Results indicate that RMS amplitude in the subtidal zone and instantaneous phase in the intertidal zone are the most sensitive to sand bodies.A comparative analysis of individual seismic attributes for sand body characterization revealed that facies-based delineation improved the accuracy of sand body identification,eff ectively capturing their contours and shapes.This method,which integrates seismic facies,single-well sedimentary microfacies,and machine learning techniques,enhances the precision of sand body characterization and off ers a novel approach to sand body prediction. 展开更多
关键词 Shunbei Area Seismic Facies Vector Quantization PCA Sandstone prediction
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Identification of Commercial Forest Tree Species Using Sentinel 2 and Planet Scope Imageries in the Usutu Forest, Eswatini
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作者 Thokozani Maxwell Ginindza 《Journal of Geographic Information System》 2025年第1期1-22,共22页
Making the distinction between different plantation tree species is crucial for creating reliable and trustworthy information, which is critical in forestry administration and upkeep. Over the years, forest delineatio... Making the distinction between different plantation tree species is crucial for creating reliable and trustworthy information, which is critical in forestry administration and upkeep. Over the years, forest delineation and mapping have been done using the conventional techniques, such as the utilization of ground truth facts together with orthophotos. These techniques have been proven to be very precise, but they are expensive, cumbersome, and challenging to employ in remote regions. To resolve this shortfall, this research investigates the potential of data from the commercial, PlanetScope CubeSat and the freely available, Sentinel 2 data from Copernicus to discriminate commercial forest tree species in the Usutu Forest, Eswatini. Two approaches for image classification, Random Forest (RF) and the Support Vector Machine (SVM) were investigated at different levels of the forest database classification which is the genus (family of tree species) and species levels. The result of the study indicates that, the Sentinel 2 images had the highest species classification accuracy compared to the PlanetScope image. Both classification methods achieved a 94% maximum OA and 0.90 kappa value at the genus level with the Sentinel 2 imagery. At the species level, the Sentinel 2 imagery again showed highly acceptable results with the SVM method, with an OA of 82%. The PlanetScope images performed badly with less than 64% OA for both RF and SVM at the genus level and poorer at the species level with a low OA figure, 47% and 53% for the SVM and RF respectively. Our results suggest that the freely available Sentinel 2 data together with the SVM method has a high potential for identifying differences between commercial tree species than the PlanetScope. The study uncovered that both classification methods are highly capable of classifying species under the gum genus group (esmi, egxu, and egxn) using both imageries. However, it was difficult to separate species types under the pine genus group, particularly discriminating the hybrid species such as pech and pell since pech is a hybrid species for pell. 展开更多
关键词 Sentinel-2 PlanetScope Random Forest Support Vector Machine SUGARCANE GENUS Species Remote Sensing
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