AIM: To generate recombinant adenoviral vector con-taining calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion gene for developing a safe, effective and HBsAg-specific therapeutic vaccine.METHODS: CRT and HB...AIM: To generate recombinant adenoviral vector con-taining calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion gene for developing a safe, effective and HBsAg-specific therapeutic vaccine.METHODS: CRT and HBsAg gene were fused using polymerase chain reaction (PCR), endonuclease diges-tion and ligation methods. The fusion gene was cloned into pENTR/D-TOPO transfer vector after the base pairs of DNA (CACC) sequence was added to the 5′ end. Adenoviral expression vector containing CRT-HBsAg fusion gene was constructed by homologous recombinan-tion. The human embryo kidney (HEK) 293A cells were transfected with linearized DNA plasmid of the recombi-nant adenoviral vector to package and amplify recombi-nant adenovirus. The recombinant adenovirus titer was characterized using the end-dilution assay. The expres-sion of the CRT/HBsAg fusion protein in Ad-CRT/HBsAg infected 293A cells was detected by Western blotting.RESULTS: The CRT-HBsAg fusion gene was char-acterized by PCR and sequencing and its length and sequence were confirmed to be accurate. The CRT-HB-sAg fusion gene recombinant pENTR/D-TOPO transfer vector was constructed. The recombinant adenoviral vector, Ad-CRT/HBsAg, was generated successfully. The titer of Ad-CRT/HBsAg was characterized as 3.9 × 1011 pfu/mL. The CRT-HBsAg fusion protein was ex-pressed by HEK 293A cells correctly. CONCLUSION: CRT/HBsAg fusion gene recombinant replication-defective adenovirus expression vector is constructed successfully and this study has provided an experimental basis for further studies of Hepatitis B vi-rus gene therapy.展开更多
In our study, support vector value contourlet transform is constructed by using support vector regression model and directional filter banks. The transform is then used to decompose source images at multi-scale, multi...In our study, support vector value contourlet transform is constructed by using support vector regression model and directional filter banks. The transform is then used to decompose source images at multi-scale, multi-direction and multi-resolution. After that, the super-resolved multi-spectral image is reconstructed by utilizing the strong learning ability of support vector regression and the correlation between multi-spectral image and panchromatic image. Finally, the super-resolved multi- spectral image and the panchromatic image are fused based on regions at different levels. Our experi- ments show that, the learning method based on support vector regression can improve the effect of super-resolution of multi-spectral image. The fused image preserves both high space resolution and spectrum information of multi-spectral image.展开更多
The geological data are constructed in vector format in geographical information system (GIS) while other data such as remote sensing images, geographical data and geochemical data are saved in raster ones. This paper...The geological data are constructed in vector format in geographical information system (GIS) while other data such as remote sensing images, geographical data and geochemical data are saved in raster ones. This paper converts the vector data into 8 bit images according to their importance to mineralization each by programming. We can communicate the geological meaning with the raster images by this method. The paper also fuses geographical data and geochemical data with the programmed strata data. The result shows that image fusion can express different intensities effectively and visualize the structure characters in 2 dimensions. Furthermore, it also can produce optimized information from multi-source data and express them more directly.展开更多
This paper investigates the problem of estimation of the wheelchair position in indoor environments with noisy mea- surements. The measuring system is based on two odometers placed on the axis of the wheels combined w...This paper investigates the problem of estimation of the wheelchair position in indoor environments with noisy mea- surements. The measuring system is based on two odometers placed on the axis of the wheels combined with a magnetic compass to determine the position and orientation. Determination of displacements is implemented by an accelerometer. Data coming from sensors are combined and used as inputs to unscented Kalman filter （UKF）. Two data fusion architectures： measurement fusion （MF） and state vector fusion （SVF） are proposed to merge the available measurements. Comparative studies of these two architectures show that the MF architecture provides states estimation with relatively less uncertainty compared to SVF. However, odometers measurements determine the position with relatively high uncertainty followed by the accelerometer measurements. Therefore, fusion in the navigation system is needed. The obtained simulation results show the effectiveness of proposed architectures.展开更多
基金Supported by Grants from National Natural Science Foundation of China, No. 30901344
文摘AIM: To generate recombinant adenoviral vector con-taining calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion gene for developing a safe, effective and HBsAg-specific therapeutic vaccine.METHODS: CRT and HBsAg gene were fused using polymerase chain reaction (PCR), endonuclease diges-tion and ligation methods. The fusion gene was cloned into pENTR/D-TOPO transfer vector after the base pairs of DNA (CACC) sequence was added to the 5′ end. Adenoviral expression vector containing CRT-HBsAg fusion gene was constructed by homologous recombinan-tion. The human embryo kidney (HEK) 293A cells were transfected with linearized DNA plasmid of the recombi-nant adenoviral vector to package and amplify recombi-nant adenovirus. The recombinant adenovirus titer was characterized using the end-dilution assay. The expres-sion of the CRT/HBsAg fusion protein in Ad-CRT/HBsAg infected 293A cells was detected by Western blotting.RESULTS: The CRT-HBsAg fusion gene was char-acterized by PCR and sequencing and its length and sequence were confirmed to be accurate. The CRT-HB-sAg fusion gene recombinant pENTR/D-TOPO transfer vector was constructed. The recombinant adenoviral vector, Ad-CRT/HBsAg, was generated successfully. The titer of Ad-CRT/HBsAg was characterized as 3.9 × 1011 pfu/mL. The CRT-HBsAg fusion protein was ex-pressed by HEK 293A cells correctly. CONCLUSION: CRT/HBsAg fusion gene recombinant replication-defective adenovirus expression vector is constructed successfully and this study has provided an experimental basis for further studies of Hepatitis B vi-rus gene therapy.
基金Supported by the National Natural Science Foundation of China(61172127)Key Research Project of Education Department of Anhui Province(KJ2010A021)
文摘In our study, support vector value contourlet transform is constructed by using support vector regression model and directional filter banks. The transform is then used to decompose source images at multi-scale, multi-direction and multi-resolution. After that, the super-resolved multi-spectral image is reconstructed by utilizing the strong learning ability of support vector regression and the correlation between multi-spectral image and panchromatic image. Finally, the super-resolved multi- spectral image and the panchromatic image are fused based on regions at different levels. Our experi- ments show that, the learning method based on support vector regression can improve the effect of super-resolution of multi-spectral image. The fused image preserves both high space resolution and spectrum information of multi-spectral image.
文摘The geological data are constructed in vector format in geographical information system (GIS) while other data such as remote sensing images, geographical data and geochemical data are saved in raster ones. This paper converts the vector data into 8 bit images according to their importance to mineralization each by programming. We can communicate the geological meaning with the raster images by this method. The paper also fuses geographical data and geochemical data with the programmed strata data. The result shows that image fusion can express different intensities effectively and visualize the structure characters in 2 dimensions. Furthermore, it also can produce optimized information from multi-source data and express them more directly.
文摘This paper investigates the problem of estimation of the wheelchair position in indoor environments with noisy mea- surements. The measuring system is based on two odometers placed on the axis of the wheels combined with a magnetic compass to determine the position and orientation. Determination of displacements is implemented by an accelerometer. Data coming from sensors are combined and used as inputs to unscented Kalman filter （UKF）. Two data fusion architectures： measurement fusion （MF） and state vector fusion （SVF） are proposed to merge the available measurements. Comparative studies of these two architectures show that the MF architecture provides states estimation with relatively less uncertainty compared to SVF. However, odometers measurements determine the position with relatively high uncertainty followed by the accelerometer measurements. Therefore, fusion in the navigation system is needed. The obtained simulation results show the effectiveness of proposed architectures.
