AIM: To generate recombinant adenoviral vector con-taining calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion gene for developing a safe, effective and HBsAg-specific therapeutic vaccine.METHODS: CRT and HB...AIM: To generate recombinant adenoviral vector con-taining calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion gene for developing a safe, effective and HBsAg-specific therapeutic vaccine.METHODS: CRT and HBsAg gene were fused using polymerase chain reaction (PCR), endonuclease diges-tion and ligation methods. The fusion gene was cloned into pENTR/D-TOPO transfer vector after the base pairs of DNA (CACC) sequence was added to the 5′ end. Adenoviral expression vector containing CRT-HBsAg fusion gene was constructed by homologous recombinan-tion. The human embryo kidney (HEK) 293A cells were transfected with linearized DNA plasmid of the recombi-nant adenoviral vector to package and amplify recombi-nant adenovirus. The recombinant adenovirus titer was characterized using the end-dilution assay. The expres-sion of the CRT/HBsAg fusion protein in Ad-CRT/HBsAg infected 293A cells was detected by Western blotting.RESULTS: The CRT-HBsAg fusion gene was char-acterized by PCR and sequencing and its length and sequence were confirmed to be accurate. The CRT-HB-sAg fusion gene recombinant pENTR/D-TOPO transfer vector was constructed. The recombinant adenoviral vector, Ad-CRT/HBsAg, was generated successfully. The titer of Ad-CRT/HBsAg was characterized as 3.9 × 1011 pfu/mL. The CRT-HBsAg fusion protein was ex-pressed by HEK 293A cells correctly. CONCLUSION: CRT/HBsAg fusion gene recombinant replication-defective adenovirus expression vector is constructed successfully and this study has provided an experimental basis for further studies of Hepatitis B vi-rus gene therapy.展开更多
In our study, support vector value contourlet transform is constructed by using support vector regression model and directional filter banks. The transform is then used to decompose source images at multi-scale, multi...In our study, support vector value contourlet transform is constructed by using support vector regression model and directional filter banks. The transform is then used to decompose source images at multi-scale, multi-direction and multi-resolution. After that, the super-resolved multi-spectral image is reconstructed by utilizing the strong learning ability of support vector regression and the correlation between multi-spectral image and panchromatic image. Finally, the super-resolved multi- spectral image and the panchromatic image are fused based on regions at different levels. Our experi- ments show that, the learning method based on support vector regression can improve the effect of super-resolution of multi-spectral image. The fused image preserves both high space resolution and spectrum information of multi-spectral image.展开更多
The geological data are constructed in vector format in geographical information system (GIS) while other data such as remote sensing images, geographical data and geochemical data are saved in raster ones. This paper...The geological data are constructed in vector format in geographical information system (GIS) while other data such as remote sensing images, geographical data and geochemical data are saved in raster ones. This paper converts the vector data into 8 bit images according to their importance to mineralization each by programming. We can communicate the geological meaning with the raster images by this method. The paper also fuses geographical data and geochemical data with the programmed strata data. The result shows that image fusion can express different intensities effectively and visualize the structure characters in 2 dimensions. Furthermore, it also can produce optimized information from multi-source data and express them more directly.展开更多
This paper investigates the problem of estimation of the wheelchair position in indoor environments with noisy mea- surements. The measuring system is based on two odometers placed on the axis of the wheels combined w...This paper investigates the problem of estimation of the wheelchair position in indoor environments with noisy mea- surements. The measuring system is based on two odometers placed on the axis of the wheels combined with a magnetic compass to determine the position and orientation. Determination of displacements is implemented by an accelerometer. Data coming from sensors are combined and used as inputs to unscented Kalman filter （UKF）. Two data fusion architectures： measurement fusion （MF） and state vector fusion （SVF） are proposed to merge the available measurements. Comparative studies of these two architectures show that the MF architecture provides states estimation with relatively less uncertainty compared to SVF. However, odometers measurements determine the position with relatively high uncertainty followed by the accelerometer measurements. Therefore, fusion in the navigation system is needed. The obtained simulation results show the effectiveness of proposed architectures.展开更多
This paper studies urban waterlog_draining decision support system based on the 4D data fusion technique.4D data includes DEM,DOQ,DLG and DRG.It supplies entire databases for waterlog forecast and analysis together wi...This paper studies urban waterlog_draining decision support system based on the 4D data fusion technique.4D data includes DEM,DOQ,DLG and DRG.It supplies entire databases for waterlog forecast and analysis together with non_spatial fundamental database.Data composition and reasoning are two key steps of 4D data fusion.Finally,this paper gives a real case: Ezhou Waterlog_Draining Decision Support System (EWDSS) with two application models,i.e.,DEM application model,water generating and draining model.展开更多
A novel detection method of support vector machine （SVM） based on fractal dimension of signals is presented. And models of SVM are made based on nugget size defects of spot welding. Classification using these traine...A novel detection method of support vector machine （SVM） based on fractal dimension of signals is presented. And models of SVM are made based on nugget size defects of spot welding. Classification using these trained SVM models is done to signals of spot welding. It is shown from effect of different SVM models that these models with different inputs. In detection of defects, these models with inputs including sound signal have a high percentage of accuracy, the detection accuracy of these models with inputs including voltage signal will reduce. So the SVM models based on fractal dimensions of sound are some optimal nondestructive detection ones. At last a comparison between SVM detection model and ANNS detection model is researched which indicates that SVM is a more effective measure than Artificial neural networks in detection of nugget size defects during spot welding.展开更多
In this work, we developed a method to efficiently optimize the kernel function for combined data of various different sources with their corresponding kernels being already available. The vectorization of the combine...In this work, we developed a method to efficiently optimize the kernel function for combined data of various different sources with their corresponding kernels being already available. The vectorization of the combined data is achieved by a weighted concatenation of the existing data vectors. This induces a kernel matrix composed of the existing kernels as blocks along the main diagonal, weighted according to the corresponding the subspaces span by the data. The induced block kernel matrix is optimized in the platform of least-squares support vector machines simultaneously as the LS-SVM is being trained, by solving an extended set of linear equations, other than a quadratically constrained quadratic programming as in a previous method. The method is tested on a benchmark dataset, and the performance is significantly improved from the highest ROC score 0.84 using individual data source to ROC score 0.92 with data fusion.展开更多
目的提升健康食品包装技术前瞻研判的精度和深度,厘清该领域创新演进路径。方法融合论文、专利与社交媒体多数据源,以双向编码器表示的主题建模(Bidirectional encoder representations from transformers for topic modeling,BERTopic...目的提升健康食品包装技术前瞻研判的精度和深度,厘清该领域创新演进路径。方法融合论文、专利与社交媒体多数据源,以双向编码器表示的主题建模(Bidirectional encoder representations from transformers for topic modeling,BERTopic)挖掘技术主题,通过设计“影响力、增长性、连贯性、创新性、不确定性/模糊性”5项指标体系筛选前沿主题,再结合社交媒体情感计算检验社会接受度,最后利用支持向量回归(Support vector regression,SVR)预测新兴技术未来发展趋势。结果生物基可降解材料、植物源抗菌涂层、RFID营养追踪、多光谱新鲜度传感和微胶囊营养靶向递送等5项技术被识别为新兴技术方向,表现出高成长性与市场渗透潜力。结论研究基于多源数据融合,提出将市场需求纳入技术识别研究,并预测新兴技术发展,为健康食品包装领域研发布局与战略决策提供了新的研究视角。展开更多
Facing the very high-resolution( VHR) image classification problem,a feature extraction and fusion framework is presented for VHR panchromatic and multispectral image classification based on deep learning techniques. ...Facing the very high-resolution( VHR) image classification problem,a feature extraction and fusion framework is presented for VHR panchromatic and multispectral image classification based on deep learning techniques. The proposed approach combines spectral and spatial information based on the fusion of features extracted from panchromatic( PAN) and multispectral( MS) images using sparse autoencoder and its deep version. There are three steps in the proposed method,the first one is to extract spatial information of PAN image,and the second one is to describe spectral information of MS image. Finally,in the third step,the features obtained from PAN and MS images are concatenated directly as a simple fusion feature. The classification is performed using the support vector machine( SVM) and the experiments carried out on two datasets with very high spatial resolution. MS and PAN images from WorldView-2 satellite indicate that the classifier provides an efficient solution and demonstrate that the fusion of the features extracted by deep learning techniques from PAN and MS images performs better than that when these techniques are used separately. In addition,this framework shows that deep learning models can extract and fuse spatial and spectral information greatly,and have huge potential to achieve higher accuracy for classification of multispectral and panchromatic images.展开更多
基金Supported by Grants from National Natural Science Foundation of China, No. 30901344
文摘AIM: To generate recombinant adenoviral vector con-taining calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion gene for developing a safe, effective and HBsAg-specific therapeutic vaccine.METHODS: CRT and HBsAg gene were fused using polymerase chain reaction (PCR), endonuclease diges-tion and ligation methods. The fusion gene was cloned into pENTR/D-TOPO transfer vector after the base pairs of DNA (CACC) sequence was added to the 5′ end. Adenoviral expression vector containing CRT-HBsAg fusion gene was constructed by homologous recombinan-tion. The human embryo kidney (HEK) 293A cells were transfected with linearized DNA plasmid of the recombi-nant adenoviral vector to package and amplify recombi-nant adenovirus. The recombinant adenovirus titer was characterized using the end-dilution assay. The expres-sion of the CRT/HBsAg fusion protein in Ad-CRT/HBsAg infected 293A cells was detected by Western blotting.RESULTS: The CRT-HBsAg fusion gene was char-acterized by PCR and sequencing and its length and sequence were confirmed to be accurate. The CRT-HB-sAg fusion gene recombinant pENTR/D-TOPO transfer vector was constructed. The recombinant adenoviral vector, Ad-CRT/HBsAg, was generated successfully. The titer of Ad-CRT/HBsAg was characterized as 3.9 × 1011 pfu/mL. The CRT-HBsAg fusion protein was ex-pressed by HEK 293A cells correctly. CONCLUSION: CRT/HBsAg fusion gene recombinant replication-defective adenovirus expression vector is constructed successfully and this study has provided an experimental basis for further studies of Hepatitis B vi-rus gene therapy.
基金Supported by the National Natural Science Foundation of China(61172127)Key Research Project of Education Department of Anhui Province(KJ2010A021)
文摘In our study, support vector value contourlet transform is constructed by using support vector regression model and directional filter banks. The transform is then used to decompose source images at multi-scale, multi-direction and multi-resolution. After that, the super-resolved multi-spectral image is reconstructed by utilizing the strong learning ability of support vector regression and the correlation between multi-spectral image and panchromatic image. Finally, the super-resolved multi- spectral image and the panchromatic image are fused based on regions at different levels. Our experi- ments show that, the learning method based on support vector regression can improve the effect of super-resolution of multi-spectral image. The fused image preserves both high space resolution and spectrum information of multi-spectral image.
文摘The geological data are constructed in vector format in geographical information system (GIS) while other data such as remote sensing images, geographical data and geochemical data are saved in raster ones. This paper converts the vector data into 8 bit images according to their importance to mineralization each by programming. We can communicate the geological meaning with the raster images by this method. The paper also fuses geographical data and geochemical data with the programmed strata data. The result shows that image fusion can express different intensities effectively and visualize the structure characters in 2 dimensions. Furthermore, it also can produce optimized information from multi-source data and express them more directly.
文摘This paper investigates the problem of estimation of the wheelchair position in indoor environments with noisy mea- surements. The measuring system is based on two odometers placed on the axis of the wheels combined with a magnetic compass to determine the position and orientation. Determination of displacements is implemented by an accelerometer. Data coming from sensors are combined and used as inputs to unscented Kalman filter （UKF）. Two data fusion architectures： measurement fusion （MF） and state vector fusion （SVF） are proposed to merge the available measurements. Comparative studies of these two architectures show that the MF architecture provides states estimation with relatively less uncertainty compared to SVF. However, odometers measurements determine the position with relatively high uncertainty followed by the accelerometer measurements. Therefore, fusion in the navigation system is needed. The obtained simulation results show the effectiveness of proposed architectures.
文摘This paper studies urban waterlog_draining decision support system based on the 4D data fusion technique.4D data includes DEM,DOQ,DLG and DRG.It supplies entire databases for waterlog forecast and analysis together with non_spatial fundamental database.Data composition and reasoning are two key steps of 4D data fusion.Finally,this paper gives a real case: Ezhou Waterlog_Draining Decision Support System (EWDSS) with two application models,i.e.,DEM application model,water generating and draining model.
基金supported by National Natural Science Foundation of China (No.50575159)Science Foundation of Ministry of Education of China (No.106049)+1 种基金Doctoral Foundation of Ministry of Education of China (No.20060056058)and Tianjin Municipal Natural Science Foundation of China (No.06YFJMJC03400).
文摘A novel detection method of support vector machine （SVM） based on fractal dimension of signals is presented. And models of SVM are made based on nugget size defects of spot welding. Classification using these trained SVM models is done to signals of spot welding. It is shown from effect of different SVM models that these models with different inputs. In detection of defects, these models with inputs including sound signal have a high percentage of accuracy, the detection accuracy of these models with inputs including voltage signal will reduce. So the SVM models based on fractal dimensions of sound are some optimal nondestructive detection ones. At last a comparison between SVM detection model and ANNS detection model is researched which indicates that SVM is a more effective measure than Artificial neural networks in detection of nugget size defects during spot welding.
文摘In this work, we developed a method to efficiently optimize the kernel function for combined data of various different sources with their corresponding kernels being already available. The vectorization of the combined data is achieved by a weighted concatenation of the existing data vectors. This induces a kernel matrix composed of the existing kernels as blocks along the main diagonal, weighted according to the corresponding the subspaces span by the data. The induced block kernel matrix is optimized in the platform of least-squares support vector machines simultaneously as the LS-SVM is being trained, by solving an extended set of linear equations, other than a quadratically constrained quadratic programming as in a previous method. The method is tested on a benchmark dataset, and the performance is significantly improved from the highest ROC score 0.84 using individual data source to ROC score 0.92 with data fusion.
文摘目的提升健康食品包装技术前瞻研判的精度和深度,厘清该领域创新演进路径。方法融合论文、专利与社交媒体多数据源,以双向编码器表示的主题建模(Bidirectional encoder representations from transformers for topic modeling,BERTopic)挖掘技术主题,通过设计“影响力、增长性、连贯性、创新性、不确定性/模糊性”5项指标体系筛选前沿主题,再结合社交媒体情感计算检验社会接受度,最后利用支持向量回归(Support vector regression,SVR)预测新兴技术未来发展趋势。结果生物基可降解材料、植物源抗菌涂层、RFID营养追踪、多光谱新鲜度传感和微胶囊营养靶向递送等5项技术被识别为新兴技术方向,表现出高成长性与市场渗透潜力。结论研究基于多源数据融合,提出将市场需求纳入技术识别研究,并预测新兴技术发展,为健康食品包装领域研发布局与战略决策提供了新的研究视角。
基金Supported by the National Natural Science Foundation of China(No.61472103,61772158,U.1711265)
文摘Facing the very high-resolution( VHR) image classification problem,a feature extraction and fusion framework is presented for VHR panchromatic and multispectral image classification based on deep learning techniques. The proposed approach combines spectral and spatial information based on the fusion of features extracted from panchromatic( PAN) and multispectral( MS) images using sparse autoencoder and its deep version. There are three steps in the proposed method,the first one is to extract spatial information of PAN image,and the second one is to describe spectral information of MS image. Finally,in the third step,the features obtained from PAN and MS images are concatenated directly as a simple fusion feature. The classification is performed using the support vector machine( SVM) and the experiments carried out on two datasets with very high spatial resolution. MS and PAN images from WorldView-2 satellite indicate that the classifier provides an efficient solution and demonstrate that the fusion of the features extracted by deep learning techniques from PAN and MS images performs better than that when these techniques are used separately. In addition,this framework shows that deep learning models can extract and fuse spatial and spectral information greatly,and have huge potential to achieve higher accuracy for classification of multispectral and panchromatic images.
