Gradient vector flow （GVF） is an effective external force for active contours, but its iso- tropic nature handicaps its performance. The recently proposed gradient vector flow in the normal direction （NGVF） is ani...Gradient vector flow （GVF） is an effective external force for active contours, but its iso- tropic nature handicaps its performance. The recently proposed gradient vector flow in the normal direction （NGVF） is anisotropic since it only keeps the diffusion along the normal direction of the isophotes; however, it has difficulties forcing a snake into long, thin boundary indentations. In this paper, a novel external force for active contours called normally generalized gradient vector flow （NGGVF） is proposed, which generalizes the NGVF formulation to include two spatially varying weighting functions. Consequently, the proposed NGGVF snake is anisotropic and would improve ac- tive contour convergence into long, thin boundary indentations while maintaining other desirable properties of the NGVF snake, such as enlarged capture range, initialization insensitivity and good convergence at concavities. The advantages on synthetic and real images are demonstrated.展开更多
Summary： Intraventricular hydrodynamics is considered an important component of cardiac function assessment. Vector flow mapping （VFM） is a novel flow visualization method to describe cardiac pathophysiological con...Summary： Intraventricular hydrodynamics is considered an important component of cardiac function assessment. Vector flow mapping （VFM） is a novel flow visualization method to describe cardiac pathophysiological condition. This study examined use of new VFM and flow field for assessment of left ventricular （LV） systolic hemodynamics in patients with simple hyperthyroidism （HT）. Thirty-seven simple HT patients were enrolled as HT group, and 38 gender- and age-matched healthy volunteers as control group. VFM model was used to analyze LV flow field at LV apical long-axis view. The follow- ing flow parameters were measured, including peak systolic velocity （Vs）, peak systolic flow （Fs）, total systolic negative flow （SQ） in LV basal, middle and apical level, velocity gradient from the apex to the aortic valve （AV）, and velocity according to half distance （V1/2）. The velocity vector in the LV cavity, stream line and vortex distribution in the two groups were observed. The results showed that there were no significant differences in the conventional parameters such as left ventricular ejection fraction （LVEF）, left ventricular end-diastolic diameter （LVEDD） and left atrium diameter （LAD） between HT group and control group （P〉0.05）. Compared with the control group, a brighter flow and more vortexes were detected in HT group. Non-uniform distribution occurred in the LV flow field, and the stream lines were discontinuous in HT group. The values of Vs and Fs in three levels, SQ in middle and basal levels, AV and V1/2 were higher in HT group than in control group （P〈0.01）. AV was positively correlated with serum free thyroxin （FT4） （r=0.48, P〈0.01）. Stepwise multiple regression analysis showed that LVEDD, FT4, and body surface area （BSA） were the influence factors of △V. The unstable left ventricular sys- tolic hydrodynamics increased in a compensatory manner in simple PIT patients. The present study in- dicated that VFM may be used for early detection of abnormal ventricle contraction in clinical settings.展开更多
In this paper, we propose a fast centerline extraction method to be used for gradient and direction vector flow of active contours. The gradient and direction vector flow is a recently reported active contour model ca...In this paper, we propose a fast centerline extraction method to be used for gradient and direction vector flow of active contours. The gradient and direction vector flow is a recently reported active contour model capable of significantly improving the image segmentation performance especially for complex object shape, by seamlessly integrating gradient vector flow and prior directional information. Since the prior directional information is provided by manual line drawing, it can be inconvenient for inexperienced users who might have difficulty in finding the best place to draw the directional lines to achieve the best segmentation performance. This paper describes a method to overcome this problem by automatically extracting centerlines to guide the users for providing the right directional information. Experimental results on synthetic and real images demonstrate the feasibility of the proposed method.展开更多
The objective of maintaining the cleanliness of the multi-segment disk amplifier in Shenguang-II(SG-II) is to reduce laser-induced damage for optics. The flow field of clean gas, which is used for the transportation o...The objective of maintaining the cleanliness of the multi-segment disk amplifier in Shenguang-II(SG-II) is to reduce laser-induced damage for optics. The flow field of clean gas, which is used for the transportation of contaminant particles,is a key factor affecting the cleanliness level in the multi-segment disk amplifier. We developed a gas–solid coupling and three-dimensional flow numerical simulation model. The three-dimensional and two-phase flow model is verified by the flow-field smog experiment and the particle concentration measurement experiment with the 130-disk amplifier in SG-II. By optimizing the boundary conditions with the same flow rate, the multi-inlet vector flow scheme can not only effectively reduce the purging time, but also prevent the reverse diffusion of contaminant particles in the multi-segment disk amplifier and the deposition of contaminant particles on the surface of the Nd:glass.展开更多
In this paper,we propose a new gradient vector flow model with advection enhancement,called advection-enhanced gradient vector flow,for calculating the external force employed in the active-contour image segmentation....In this paper,we propose a new gradient vector flow model with advection enhancement,called advection-enhanced gradient vector flow,for calculating the external force employed in the active-contour image segmentation.The proposed model is mainly inspired by the functional derivative of an adaptive total variation regularizer whose minimizer is expected to be able to effectively preserve the desired object boundary.More specifically,by incorporating an additional advection term into the usual gradient vector flow model,the resulting external force can much better help the active contour to recover missing edges,to converge to a narrow and deep concavity,and to preserve weak edges.Numerical experiments are performed to demonstrate the high performance of the newly proposed model.展开更多
Based on K ε two equation turbulence model,the air distribution and contamination field under different conditions was numerically simulated in a vector flow cleanroom.Special mesh system was introduced to deal wit...Based on K ε two equation turbulence model,the air distribution and contamination field under different conditions was numerically simulated in a vector flow cleanroom.Special mesh system was introduced to deal with the quarter circle shaped inlets.Model experiments were also made.By analysis of numerical as well as experimental results,we made some predictions about flow characteristics,contaminant control effect and ventilation performance of this energy saving cleanroom.It has been proved that the vector flow can meet class 100 of Fed. St.with 13 to 12 conventional air change per hour,and reduce layer height and simplify air clean system.展开更多
In this paper the transient two-phase flow equations and their eigenvalues are first introduced. The flux vector is then split into subvectors which just contain a specially signed eigenvalue. Using one-sided spatial ...In this paper the transient two-phase flow equations and their eigenvalues are first introduced. The flux vector is then split into subvectors which just contain a specially signed eigenvalue. Using one-sided spatial difference operators finite difference equations and their solutions are obtained. Finally comparison with experiment shows the predicted results produce good agreement with experimental data.展开更多
The gas-liquid-solid three-phase mixed flow is the most general in multiphase mixed transportation. It is significant to exactly solve the coupling hydraulic transient problems of this type of multiphase mixed flow in...The gas-liquid-solid three-phase mixed flow is the most general in multiphase mixed transportation. It is significant to exactly solve the coupling hydraulic transient problems of this type of multiphase mixed flow in pipelines. Presently, the method of characteristics is widely used to solve classical hydraulic transient problems. However, when it is used to solve coupling hydraulic transient problems, excessive interpolation errors may be introduced into the results due to unavoidable multiwave interpolated calculations. To deal with the problem, a finite difference scheme based on the Steger- Warming flux vector splitting is proposed. A flux vector splitting scheme is established for the coupling hydraulic transient model of gas-liquid-solid three-phase mixed flow in the pipelines. The flux subvectors are then discretized by the Lax-Wendroff central difference scheme and the Warming-Beam upwind difference scheme with second-order precision in both time and space. Under the Rankine-Hugoniot conditions and the corresponding boundary conditions, an effective solution to those points located at the boundaries is developed, which can avoid the problem beyond the calculation region directly induced by the second-order discrete technique. Numerical and experimental verifications indicate that the proposed scheme has several desirable advantages including high calculation precision, excellent shock wave capture capability without false numerical oscillation, low sensitivity to the Courant number, and good stability.展开更多
In Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, the fuel debris formed in the Reactor Pressure Vessel (RPV) and Primary Containment Vessel (PCV) at Unit 1</span><span style="font-family:Verdan...In Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, the fuel debris formed in the Reactor Pressure Vessel (RPV) and Primary Containment Vessel (PCV) at Unit 1</span><span style="font-family:Verdana;"> </span><span style="font-family:Verdana;">-</span><span style="font-family:Verdana;"> </span><span style="font-family:Verdana;">3. To accelerate and decide further decommissioning steps of the FDNPP, it is crucial to obtain realistic information of the debris and localize contaminated water leakage from PCV. Due to high radiation and dark environment inside the PCV, investigating instruments and techniques should necessarily to meet specification of radiation resistance, waterproofness, dust resistance and so on. This study focuses on development of ultrasonic measurement system using a couple of sectorial array sensors to localize contaminated water leakage and visualize shape of object that repre</span><span style="font-family:Verdana;">- </span><span style="font-family:Verdana;">senting fuel debris, simultaneously. In this study, Total Focusing Method</span><span style="font-family:Verdana;"> (TFM) and Ultrasonic Velocity Profiler (UVP) methods are considered to visualize object shape and flow pattern around it, respectively. To demonstrate applicability and reliability of developed measurement system with sectorial array sensors, a mock-up experiment result</span><span style="font-family:Verdana;"> </span><span style="font-family:Verdana;">of simulated water leakage and fuel debris shape were discussed in this paper.展开更多
A primary air jet vectoring control system with a novel synthetic jet actuator （SJA） is presented and simulated numerically. The results show that, in comparison with an existing traditional synthetic jet actuator, ...A primary air jet vectoring control system with a novel synthetic jet actuator （SJA） is presented and simulated numerically. The results show that, in comparison with an existing traditional synthetic jet actuator, which is able to perform the duty of either ＂push＂ or ＂pull＂, one novel synthetic jet actuator can fulfill both ＂push＂ and ＂pull＂ functions to vector the primary jet by shifting a slide block inside it. Therefore, because the new actuator possesses greater efficiency, it has potentiality to replace the existing one in various appli- cations, such as thrust vectoring and the reduction of thermal signature. Moreover, as the novel actuator can fulfill those functions that the existing one can not, it may well be expected to popularize it into more flow control systems.展开更多
Based on the theory of EHD （electronhydrodynamic）, a simplified volume force model is applied to simulation to analyze the traits of plasma flow control in flow field, in which the cold plasma is generated by a DBD ...Based on the theory of EHD （electronhydrodynamic）, a simplified volume force model is applied to simulation to analyze the traits of plasma flow control in flow field, in which the cold plasma is generated by a DBD （dielectric-barrier-discharge） actuator. With the para- electric action of volume force in electric field, acceleration characteristics of the plasma flow are investigated for different excitation intensities of RF （radio frequency） power for the actuator. Furthermore, the plasma acceleration leads to an asymmetric distribution of flow field, and hence induces the deflection of jet plume, then results in a significant deflection angle of 6.26° thrustvectoring effect. It appears that the plasma flow control technology is a new tentative method for the thrust-vectoring control of a space vehicle.展开更多
A primary jet vectoring using synthetic jet actuators with different exit configurations was investigated, and the main physical factors influencing jet vectoring were analyzed and summarized. The physical factors of ...A primary jet vectoring using synthetic jet actuators with different exit configurations was investigated, and the main physical factors influencing jet vectoring were analyzed and summarized. The physical factors of the pressure difference, the location and area of the lower pressure region, the component of the synthetic jet momentum and the entrainment ratio of the synthetic jet flow to primary jet flow directly control the vectoring force and the vectoring angle. Three characteristic parameters of the synthetic jet contribute to the pressure difference and the area of the lower pressure region Both the extension step and slope angle of the actuator exit have functions of regulating the location of the lower pressure region, the area of the lower pressure region, and the entrainment ratio of the synthetic jet flow to primary jet flow. The slope angle of the actuator exit has additional functions of regulating the component of the synthetic jet momentum. Based upon analyzing the physical factors of jet vectoring control with synthetic jets, the source variables of the physical factors were established. A preparatory control model of jet vectoring using synthetic jet actuator was presented, and it has the benefit of explaining the efficiency of jet vectoring using synthetic jet actuator with source variables at different values, and it indicates the optimal actuator is taking full advantage of the regulating function.展开更多
Myostatin （MSTN） is a negative regulator of skeletal muscle growth, in order to study the effect of inhibition MSTN expression on the proliferation of bovine skeletal muscle satellite cells, we constructed co-expres...Myostatin （MSTN） is a negative regulator of skeletal muscle growth, in order to study the effect of inhibition MSTN expression on the proliferation of bovine skeletal muscle satellite cells, we constructed co-expression vector pcDNA3.1-Pro- MSTNshRNA, transfected it into muscle satellite cells by Liposome 2000, and detected cell proliferation changes by CCK-8 method and flow cytometry after 48 h. The expressions of P21 and CDK2 were detected by Western blot and real-time PCR. The results showed that the cell vitality of experimental groups significantly increased than that of the negative control, and cells in S phase also increased significantly （P〈0.05）. After knocked down MSTN gene, P21 expression decreased （P〈0.05）, but CDK2 gene expression increased （P〈0.05）. These results indicated that MSTN gene expression was associated with P21 and CDK2, the proliferation of skeletal muscle satellite cells could be promoted while MSTN was inhibited, which provided a theoretical basis for the study on transgenic cattle.展开更多
ABSTRACT Current computerized pulse diagnosis is mainly based on pressure and photoelectric signal. Considering the richness and complication of pulse diagnosis information, it is valuable to explore the feasibility o...ABSTRACT Current computerized pulse diagnosis is mainly based on pressure and photoelectric signal. Considering the richness and complication of pulse diagnosis information, it is valuable to explore the feasibility of novel types of signal and to develop appropriate feature representation for diagnosis. In this paper, we present a study on computerized pulse diagnosis based on blood flow velocity signal. First, the blood flow velocity signal is collected using Doppler ultrasound device and preprocessed. Then, by locating the fiducial points, we extract the spatial features of blood flow velocity signal, and further present a Hilbert-Huang transform-based method for spectrum feature extraction. Finally, support vector machine is applied for computerized pulse diagnosis. Experiment results show that the proposed method is effective and promising in distinguishing healthy people from patients with cho- lecystitis or nephritis.展开更多
基金Supported by the National Natural Science Foundation of China(60805004)the State Key Lab of Space Medicine Fundamen-tals and Application(SMFA09A16)
文摘Gradient vector flow （GVF） is an effective external force for active contours, but its iso- tropic nature handicaps its performance. The recently proposed gradient vector flow in the normal direction （NGVF） is anisotropic since it only keeps the diffusion along the normal direction of the isophotes; however, it has difficulties forcing a snake into long, thin boundary indentations. In this paper, a novel external force for active contours called normally generalized gradient vector flow （NGGVF） is proposed, which generalizes the NGVF formulation to include two spatially varying weighting functions. Consequently, the proposed NGGVF snake is anisotropic and would improve ac- tive contour convergence into long, thin boundary indentations while maintaining other desirable properties of the NGVF snake, such as enlarged capture range, initialization insensitivity and good convergence at concavities. The advantages on synthetic and real images are demonstrated.
基金supported by Independent Innovation Fund of Huazhong University of Science and Technology for Clinical Skills,China(No.2015-01-18-53028)
文摘Summary： Intraventricular hydrodynamics is considered an important component of cardiac function assessment. Vector flow mapping （VFM） is a novel flow visualization method to describe cardiac pathophysiological condition. This study examined use of new VFM and flow field for assessment of left ventricular （LV） systolic hemodynamics in patients with simple hyperthyroidism （HT）. Thirty-seven simple HT patients were enrolled as HT group, and 38 gender- and age-matched healthy volunteers as control group. VFM model was used to analyze LV flow field at LV apical long-axis view. The follow- ing flow parameters were measured, including peak systolic velocity （Vs）, peak systolic flow （Fs）, total systolic negative flow （SQ） in LV basal, middle and apical level, velocity gradient from the apex to the aortic valve （AV）, and velocity according to half distance （V1/2）. The velocity vector in the LV cavity, stream line and vortex distribution in the two groups were observed. The results showed that there were no significant differences in the conventional parameters such as left ventricular ejection fraction （LVEF）, left ventricular end-diastolic diameter （LVEDD） and left atrium diameter （LAD） between HT group and control group （P〉0.05）. Compared with the control group, a brighter flow and more vortexes were detected in HT group. Non-uniform distribution occurred in the LV flow field, and the stream lines were discontinuous in HT group. The values of Vs and Fs in three levels, SQ in middle and basal levels, AV and V1/2 were higher in HT group than in control group （P〈0.01）. AV was positively correlated with serum free thyroxin （FT4） （r=0.48, P〈0.01）. Stepwise multiple regression analysis showed that LVEDD, FT4, and body surface area （BSA） were the influence factors of △V. The unstable left ventricular sys- tolic hydrodynamics increased in a compensatory manner in simple PIT patients. The present study in- dicated that VFM may be used for early detection of abnormal ventricle contraction in clinical settings.
文摘In this paper, we propose a fast centerline extraction method to be used for gradient and direction vector flow of active contours. The gradient and direction vector flow is a recently reported active contour model capable of significantly improving the image segmentation performance especially for complex object shape, by seamlessly integrating gradient vector flow and prior directional information. Since the prior directional information is provided by manual line drawing, it can be inconvenient for inexperienced users who might have difficulty in finding the best place to draw the directional lines to achieve the best segmentation performance. This paper describes a method to overcome this problem by automatically extracting centerlines to guide the users for providing the right directional information. Experimental results on synthetic and real images demonstrate the feasibility of the proposed method.
基金supported by the National Natural Science Foundation of China (Grant No. 61505228)
文摘The objective of maintaining the cleanliness of the multi-segment disk amplifier in Shenguang-II(SG-II) is to reduce laser-induced damage for optics. The flow field of clean gas, which is used for the transportation of contaminant particles,is a key factor affecting the cleanliness level in the multi-segment disk amplifier. We developed a gas–solid coupling and three-dimensional flow numerical simulation model. The three-dimensional and two-phase flow model is verified by the flow-field smog experiment and the particle concentration measurement experiment with the 130-disk amplifier in SG-II. By optimizing the boundary conditions with the same flow rate, the multi-inlet vector flow scheme can not only effectively reduce the purging time, but also prevent the reverse diffusion of contaminant particles in the multi-segment disk amplifier and the deposition of contaminant particles on the surface of the Nd:glass.
基金supported by the Ministry of Science and Technology of Taiwan under grants MOST 106-2115-M-005-005-MY2(Po-Wen Hsieh),MOST 107-2811-M-008-007(Pei-Chiang Shao)MOST 106-2115-M-008-014-MY2(Suh-Yuh Yang).
文摘In this paper,we propose a new gradient vector flow model with advection enhancement,called advection-enhanced gradient vector flow,for calculating the external force employed in the active-contour image segmentation.The proposed model is mainly inspired by the functional derivative of an adaptive total variation regularizer whose minimizer is expected to be able to effectively preserve the desired object boundary.More specifically,by incorporating an additional advection term into the usual gradient vector flow model,the resulting external force can much better help the active contour to recover missing edges,to converge to a narrow and deep concavity,and to preserve weak edges.Numerical experiments are performed to demonstrate the high performance of the newly proposed model.
文摘Based on K ε two equation turbulence model,the air distribution and contamination field under different conditions was numerically simulated in a vector flow cleanroom.Special mesh system was introduced to deal with the quarter circle shaped inlets.Model experiments were also made.By analysis of numerical as well as experimental results,we made some predictions about flow characteristics,contaminant control effect and ventilation performance of this energy saving cleanroom.It has been proved that the vector flow can meet class 100 of Fed. St.with 13 to 12 conventional air change per hour,and reduce layer height and simplify air clean system.
文摘In this paper the transient two-phase flow equations and their eigenvalues are first introduced. The flux vector is then split into subvectors which just contain a specially signed eigenvalue. Using one-sided spatial difference operators finite difference equations and their solutions are obtained. Finally comparison with experiment shows the predicted results produce good agreement with experimental data.
基金supported by the Natural Science Foundation Project of CQ CSTC (No. 2010BB7421)
文摘The gas-liquid-solid three-phase mixed flow is the most general in multiphase mixed transportation. It is significant to exactly solve the coupling hydraulic transient problems of this type of multiphase mixed flow in pipelines. Presently, the method of characteristics is widely used to solve classical hydraulic transient problems. However, when it is used to solve coupling hydraulic transient problems, excessive interpolation errors may be introduced into the results due to unavoidable multiwave interpolated calculations. To deal with the problem, a finite difference scheme based on the Steger- Warming flux vector splitting is proposed. A flux vector splitting scheme is established for the coupling hydraulic transient model of gas-liquid-solid three-phase mixed flow in the pipelines. The flux subvectors are then discretized by the Lax-Wendroff central difference scheme and the Warming-Beam upwind difference scheme with second-order precision in both time and space. Under the Rankine-Hugoniot conditions and the corresponding boundary conditions, an effective solution to those points located at the boundaries is developed, which can avoid the problem beyond the calculation region directly induced by the second-order discrete technique. Numerical and experimental verifications indicate that the proposed scheme has several desirable advantages including high calculation precision, excellent shock wave capture capability without false numerical oscillation, low sensitivity to the Courant number, and good stability.
文摘In Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, the fuel debris formed in the Reactor Pressure Vessel (RPV) and Primary Containment Vessel (PCV) at Unit 1</span><span style="font-family:Verdana;"> </span><span style="font-family:Verdana;">-</span><span style="font-family:Verdana;"> </span><span style="font-family:Verdana;">3. To accelerate and decide further decommissioning steps of the FDNPP, it is crucial to obtain realistic information of the debris and localize contaminated water leakage from PCV. Due to high radiation and dark environment inside the PCV, investigating instruments and techniques should necessarily to meet specification of radiation resistance, waterproofness, dust resistance and so on. This study focuses on development of ultrasonic measurement system using a couple of sectorial array sensors to localize contaminated water leakage and visualize shape of object that repre</span><span style="font-family:Verdana;">- </span><span style="font-family:Verdana;">senting fuel debris, simultaneously. In this study, Total Focusing Method</span><span style="font-family:Verdana;"> (TFM) and Ultrasonic Velocity Profiler (UVP) methods are considered to visualize object shape and flow pattern around it, respectively. To demonstrate applicability and reliability of developed measurement system with sectorial array sensors, a mock-up experiment result</span><span style="font-family:Verdana;"> </span><span style="font-family:Verdana;">of simulated water leakage and fuel debris shape were discussed in this paper.
基金National Natural Science Foundation of China (90205016, 50176055)
文摘A primary air jet vectoring control system with a novel synthetic jet actuator （SJA） is presented and simulated numerically. The results show that, in comparison with an existing traditional synthetic jet actuator, which is able to perform the duty of either ＂push＂ or ＂pull＂, one novel synthetic jet actuator can fulfill both ＂push＂ and ＂pull＂ functions to vector the primary jet by shifting a slide block inside it. Therefore, because the new actuator possesses greater efficiency, it has potentiality to replace the existing one in various appli- cations, such as thrust vectoring and the reduction of thermal signature. Moreover, as the novel actuator can fulfill those functions that the existing one can not, it may well be expected to popularize it into more flow control systems.
基金supported by National Natural Science Foundation of China (No.90716025)
文摘Based on the theory of EHD （electronhydrodynamic）, a simplified volume force model is applied to simulation to analyze the traits of plasma flow control in flow field, in which the cold plasma is generated by a DBD （dielectric-barrier-discharge） actuator. With the para- electric action of volume force in electric field, acceleration characteristics of the plasma flow are investigated for different excitation intensities of RF （radio frequency） power for the actuator. Furthermore, the plasma acceleration leads to an asymmetric distribution of flow field, and hence induces the deflection of jet plume, then results in a significant deflection angle of 6.26° thrustvectoring effect. It appears that the plasma flow control technology is a new tentative method for the thrust-vectoring control of a space vehicle.
基金Project supported by the National Natural Science Foundation of China(Nos.90205016 and 50176055)
文摘A primary jet vectoring using synthetic jet actuators with different exit configurations was investigated, and the main physical factors influencing jet vectoring were analyzed and summarized. The physical factors of the pressure difference, the location and area of the lower pressure region, the component of the synthetic jet momentum and the entrainment ratio of the synthetic jet flow to primary jet flow directly control the vectoring force and the vectoring angle. Three characteristic parameters of the synthetic jet contribute to the pressure difference and the area of the lower pressure region Both the extension step and slope angle of the actuator exit have functions of regulating the location of the lower pressure region, the area of the lower pressure region, and the entrainment ratio of the synthetic jet flow to primary jet flow. The slope angle of the actuator exit has additional functions of regulating the component of the synthetic jet momentum. Based upon analyzing the physical factors of jet vectoring control with synthetic jets, the source variables of the physical factors were established. A preparatory control model of jet vectoring using synthetic jet actuator was presented, and it has the benefit of explaining the efficiency of jet vectoring using synthetic jet actuator with source variables at different values, and it indicates the optimal actuator is taking full advantage of the regulating function.
基金Supported by the Major Special Projects of New Product Training of Transgenic Organisms(zx080072008-2008)
文摘Myostatin （MSTN） is a negative regulator of skeletal muscle growth, in order to study the effect of inhibition MSTN expression on the proliferation of bovine skeletal muscle satellite cells, we constructed co-expression vector pcDNA3.1-Pro- MSTNshRNA, transfected it into muscle satellite cells by Liposome 2000, and detected cell proliferation changes by CCK-8 method and flow cytometry after 48 h. The expressions of P21 and CDK2 were detected by Western blot and real-time PCR. The results showed that the cell vitality of experimental groups significantly increased than that of the negative control, and cells in S phase also increased significantly （P〈0.05）. After knocked down MSTN gene, P21 expression decreased （P〈0.05）, but CDK2 gene expression increased （P〈0.05）. These results indicated that MSTN gene expression was associated with P21 and CDK2, the proliferation of skeletal muscle satellite cells could be promoted while MSTN was inhibited, which provided a theoretical basis for the study on transgenic cattle.
文摘ABSTRACT Current computerized pulse diagnosis is mainly based on pressure and photoelectric signal. Considering the richness and complication of pulse diagnosis information, it is valuable to explore the feasibility of novel types of signal and to develop appropriate feature representation for diagnosis. In this paper, we present a study on computerized pulse diagnosis based on blood flow velocity signal. First, the blood flow velocity signal is collected using Doppler ultrasound device and preprocessed. Then, by locating the fiducial points, we extract the spatial features of blood flow velocity signal, and further present a Hilbert-Huang transform-based method for spectrum feature extraction. Finally, support vector machine is applied for computerized pulse diagnosis. Experiment results show that the proposed method is effective and promising in distinguishing healthy people from patients with cho- lecystitis or nephritis.
