Tongluojiunao （TLJN） is an herbal medicine consisting of two main components, geniposide and ginsenoside Rg1. TLJN has been shown to protect primary cultured hippocampal neurons. How-ever, its mechanism of action re...Tongluojiunao （TLJN） is an herbal medicine consisting of two main components, geniposide and ginsenoside Rg1. TLJN has been shown to protect primary cultured hippocampal neurons. How-ever, its mechanism of action remains unclear. In the present study, primary cultured hippocampal neurons treated with Aβ1-42 （10 μmol/L） signiifcantly increased the release of lactate dehydroge-nase, which was markedly reduced by TLJN （2 μL/mL）, speciifcally by the component geniposide （26 μmol/L）, but not ginsenoside Rg1 （2.5 μmol/L）. hTe estrogen receptor inhibitor, ICI182780 （1 μmol/L）, did not block TLJN-or geniposide-mediated decrease of lactate dehydrogenase under Aβ1-42-exposed conditions. However, the phosphatidyl inositol 3-kinase or mitogen-activated protein kinase pathway inhibitor, LY294002 （50 μmol/L） or U0126 （10 μmol/L）, respectively blo cked the decrease of lactate dehydrogenase mediated by TLJN or geniposide. hTerefore, these results suggest that the non-classical estrogen pathway （i.e., phosphatidyl inositol 3-kinase or mitogen-activated protein kinase） is involved in the neuroprotective effect of TLJN, speciifcally its component, geniposide, against Aβ1-42-mediated cell death in primary cultured hippocampal neurons.展开更多
Activated carbon-supported bimetallic cerium-man- ganese （Ce-Mn/AC） materials were prepared by impregnation method to study the effect of calcination temperature on the structure and adsorption performance of absorb...Activated carbon-supported bimetallic cerium-man- ganese （Ce-Mn/AC） materials were prepared by impregnation method to study the effect of calcination temperature on the structure and adsorption performance of absorbents. The obtained materials were characterized by using X-ray diffraction （XRD）, scanning electron microscope （SEM）, N2 adsorption-desorption iso- therm, Fourier transformed infrared （FT-IR）, and X-ray photoelectron spectroscopy （XPS）. As the results showed, the diffraction peaks of CeO2 decreased and even disappeared; Mn species were transformed from Mn3O4 to Mn2O3 on the surface of Ce-Mn/AC; the BET specific surface area increased first and then decreased on the elevating calcination temperature; the number of acid functions of AC was reduced after being modified by cerium and manganese All these changes were directly attributed to the synergistic effects between MnOx and CeO2. AC800 exhibited the best phenol adsorption capacity. The adsorption mechanism of phenol on Ce-Mn/AC was discussed with hydrophilic （hydrophobic） interaction and hard and soft acid-base theory.展开更多
Using acetylcholinesterase (AChE) histochemical technique, Shute and Lewis (1960) traced nervus intermedius, which innervated submandibular gland and sublingual gland, to two strongly AChE positive nuclei situated res...Using acetylcholinesterase (AChE) histochemical technique, Shute and Lewis (1960) traced nervus intermedius, which innervated submandibular gland and sublingual gland, to two strongly AChE positive nuclei situated respectively at the medial and lateral sides of the genu of the facial nerve and regarded these two nuclei as the salivatory nuclei. However, recently, using retrograde transportation of horseradish pe-展开更多
基金supported by the National Natural Science Foundation of China No.81072901the New Teacher Fund for Doctor Station,Ministry of Education,No.20120013110013+1 种基金grants from the Nautical Traditional Chinese Medicine Discipline,No.522/0100604054grants from the Nautical Traditional Chinese Medicine Collaborative Innovation Center,No.522/0100604299
文摘Tongluojiunao （TLJN） is an herbal medicine consisting of two main components, geniposide and ginsenoside Rg1. TLJN has been shown to protect primary cultured hippocampal neurons. How-ever, its mechanism of action remains unclear. In the present study, primary cultured hippocampal neurons treated with Aβ1-42 （10 μmol/L） signiifcantly increased the release of lactate dehydroge-nase, which was markedly reduced by TLJN （2 μL/mL）, speciifcally by the component geniposide （26 μmol/L）, but not ginsenoside Rg1 （2.5 μmol/L）. hTe estrogen receptor inhibitor, ICI182780 （1 μmol/L）, did not block TLJN-or geniposide-mediated decrease of lactate dehydrogenase under Aβ1-42-exposed conditions. However, the phosphatidyl inositol 3-kinase or mitogen-activated protein kinase pathway inhibitor, LY294002 （50 μmol/L） or U0126 （10 μmol/L）, respectively blo cked the decrease of lactate dehydrogenase mediated by TLJN or geniposide. hTerefore, these results suggest that the non-classical estrogen pathway （i.e., phosphatidyl inositol 3-kinase or mitogen-activated protein kinase） is involved in the neuroprotective effect of TLJN, speciifcally its component, geniposide, against Aβ1-42-mediated cell death in primary cultured hippocampal neurons.
基金Supported by the National Natural Science Foundation of China(21543016)the Science and Technology Program of Shaanxi Province(2015 GY114)the Industrialization Cultivation Program of Shaanxi Province Education Department(15JF035)
文摘Activated carbon-supported bimetallic cerium-man- ganese （Ce-Mn/AC） materials were prepared by impregnation method to study the effect of calcination temperature on the structure and adsorption performance of absorbents. The obtained materials were characterized by using X-ray diffraction （XRD）, scanning electron microscope （SEM）, N2 adsorption-desorption iso- therm, Fourier transformed infrared （FT-IR）, and X-ray photoelectron spectroscopy （XPS）. As the results showed, the diffraction peaks of CeO2 decreased and even disappeared; Mn species were transformed from Mn3O4 to Mn2O3 on the surface of Ce-Mn/AC; the BET specific surface area increased first and then decreased on the elevating calcination temperature; the number of acid functions of AC was reduced after being modified by cerium and manganese All these changes were directly attributed to the synergistic effects between MnOx and CeO2. AC800 exhibited the best phenol adsorption capacity. The adsorption mechanism of phenol on Ce-Mn/AC was discussed with hydrophilic （hydrophobic） interaction and hard and soft acid-base theory.
文摘Using acetylcholinesterase (AChE) histochemical technique, Shute and Lewis (1960) traced nervus intermedius, which innervated submandibular gland and sublingual gland, to two strongly AChE positive nuclei situated respectively at the medial and lateral sides of the genu of the facial nerve and regarded these two nuclei as the salivatory nuclei. However, recently, using retrograde transportation of horseradish pe-
