Background The co-existence of Type 2 diabetes mellitus(T2DM)and hypertension presents a significant clinical challenge due to their synergistic detrimental effects on cardiovascular outcomes.Conventional combination ...Background The co-existence of Type 2 diabetes mellitus(T2DM)and hypertension presents a significant clinical challenge due to their synergistic detrimental effects on cardiovascular outcomes.Conventional combination therapies often fall short in comprehensively addressing the intertwined pathophysiologies,particularly vascular endothelial dysfunction.This study aimed to evaluate the efficacy of a novel regimen combining sitagliptin-metformin with nifedipine in managing blood pressure,blood glucose,and vascular function in this high-risk population.Methods 150 patients with T2DM and hypertension were randomly assigned to either the control group(n=75)treated with metformin and nifedipine,or the experimental group(n=75)treated with sitagliptin-metformin compound preparation combined with nifedipine.Changes in blood pressure,blood glucose levels,flow-mediated dilation(FMD)and nitroglycerin-mediated dilation(NMD)of the brachial artery,and serum levels of biomarkers[nitric oxide(NO),endothelin-1(ET-1)]were compared before and after treatment.The incidence of adverse reactions during treatment was also monitored.Results After treatment,both groups showed significant reductions in blood pressure,blood glucose parameters,and ET-1 levels compared to baseline.The experimental group demonstrated significantly lower values in these assessments at the same time points than control group(P<0.05).Conversely,FMD,NMD,and NO levels increased significantly in both groups after treatment,with experimental group showing significantly higher values than control group(P<0.05).There was no statistically significant difference in the incidence of adverse reactions between the two groups(P>0.05).Conclusions The combination of sitagliptinmetformin compound preparation and nifedipine significantly enhanced blood pressure control and blood glucose control and exerted a positive regulatory effect on vascular endothelial function in patients with T2DM and hypertension,demonstrating high clinical value for widespread application.展开更多
Background Coronary heart disease with myocardial ischemia is closely associated with vascular endothelial dysfunction,dyslipidemia,and chronic inflammatory response.Atorvastatin,a classic lipid-lowering agent,exhibit...Background Coronary heart disease with myocardial ischemia is closely associated with vascular endothelial dysfunction,dyslipidemia,and chronic inflammatory response.Atorvastatin,a classic lipid-lowering agent,exhibits pleiotropic effects including plaque stabilization,endothelial function improvement,and anti-inflammatory actions.Nicorandil,as a potassium channel opener with additional nitrate-like effects,directly dilates coronary arteries and improves myocardial microcirculation.Theoretically,the two drugs may synergistically enhance myocardial perfusion and endothelial function through distinct mechanisms.However,clinical evidence regarding the comprehensive impact of their combination on vascular endothelial function,blood lipid profiles,and systemic inflammatory status in patients with coronary heart disease and myocardial ischemia remains insufficient.Further studies are warranted to clarify their synergistic effects and clinical value.Methods A total of 60 patients with coronary heart disease and myocardial ischemia admitted to our hospital from January 2023 to October 2024 were enrolled and randomly divided into combination group and control group using a random number table,with 30 patients in each group.The control group received atorvastatin treatment,while the combination group received nicorandil combined with atorvastatin.After 12 weeks of continuous treatment,the clinical efficacy,vascular endothelial function,blood lipid profiles,inflammatory factors,and adverse reactions were compared between the two groups.Results The total effective rate in the combination group(93.33%)was significantly higher than that in the control group(70.00%)(P<0.05).After treatment,serum endothelin(ET)level was lower in the combination group than in the control group(P<0.05),while nitric oxide(NO)level was higher(P<0.05).In the combination group,the levels of total cholesterol(TC),triglyceride(TG),and low-density lipoprotein cholesterol(LDL-C)were all lower than those in the control group(P<0.05),while high-density lipoprotein cholesterol(HDL-C)level was higher(P<0.05).Levels of C-reactive protein(CRP),interleukin(IL)-8,and IL-6 in the combination group were also lower compared with the control group(P<0.05).No significant difference was observed in the incidence of adverse reactions between the two groups(P>0.05).Conclusions The combination of nicorandil and atorvastatin demonstrated certain short-term therapeutic effects in patients with coronary heart disease and myocardial ischemia.It not only effectively improves vascular endothelial function and regulates lipid metabolism but also suppresses the levels of inflammatory factors,with a favorable safety profile.展开更多
OBJECTIVE:To evaluate the extent of vascular endothelial dysfunction and preliminary identify serum protein biomarkers associated with obese individuals at risk for cardiovascular disease(CVD).METHODS:Fifteen obese vo...OBJECTIVE:To evaluate the extent of vascular endothelial dysfunction and preliminary identify serum protein biomarkers associated with obese individuals at risk for cardiovascular disease(CVD).METHODS:Fifteen obese volunteers with the phlegmdampness constitution or balanced constitution were recruited for this study respectively.The clinical baseline data was collected,and the vascular endothelial function was evaluated using the EndoPAT?.Blood samples were collected for the serum proteome analysis.The differences in the serum protein expression levels between the two groups were detected and the protein interaction network analysis,correlation analysis,receiver operating characteristic(ROC)curve analysis,and random forest model investigation were conducted.RESULTS:There were no statistical differences found in the baseline data.For vascular endothelial function,the reactive hyperemia index(RHI)of the phlegm-dampness constitution obese group was significantly lower than that of the balanced constitution obese group(1.46±0.30 vs 2.82±0.78,P<0.0001),indicating vascular endothelial dysfunction.There are 66 differentially expressed serum proteins between the two groups.apolipoprotein A2(Apo A2),angiotensin-converting enzyme 2(ACE-2),interleukin-33(IL-33),and forkhead box P3(FoxP3)showed significant differences and area under curve values of their ROC curves were greater than 0.7 and correlated significantly with RHI.CONCLUSION:Vascular endothelial dysfunction was present in the phlegm-dampness constitution obese group.Thus,alterations in the expression levels of key serum proteins,including Apo A2,ACE-2,IL-33,and Fox P3 could serve as potential biomarkers in the obese population at risk of CVD.展开更多
Objective Cardiopulmonary bypass (CPB) and its related ischemia reperfusion injury may cause endothelial cell injury. To study the protective effects of sodium ferulate in vascular endothelial function during CPB by t...Objective Cardiopulmonary bypass (CPB) and its related ischemia reperfusion injury may cause endothelial cell injury. To study the protective effects of sodium ferulate in vascular endothelial function during CPB by testing the changes of vascular endothelial cell(CEC) ,nitric oxide(NO) and endothelin-1 (ET-1) in children with congenital heart disease. Methods Sixty patients展开更多
Objectives To understand the effect of carvedilol on the coronary vascular endothelial function of the patients with coronary heart disease after percutaneous transluminal coronary angioplasty (PTCA). Methods 51cases,...Objectives To understand the effect of carvedilol on the coronary vascular endothelial function of the patients with coronary heart disease after percutaneous transluminal coronary angioplasty (PTCA). Methods 51cases, having one or more than two branches narrow ( ≥ 70% ) , were diagnosed by coronary angiography. These patients were divided randomly into carvedilol group (n = 28) and control group ( n = 23) who did not take carvedilol. Endothelin (ET) and nitro dioxide (NO) levels of peripheral blood were measured before and after PTCA, before and after two weeks by taking earvedilol. Results Compared with the ET and NO levels before PTCA, ET were markedly increased and NO were decreased after PTCA (p <0. 05) ; compared with the ET and NO levels before taking carvedilol, ET were decreased and NO were increased after two week (p <0.05 ) , but the ET and NO levels of the control group did not change in the period of two weeks observation (p > 0.05). Conclusions Carvedilol may improve the coronary vascular endothelial function after PTCA.展开更多
Atherosclerosis occurs as a result of organized processes that include vascular endothelial dysfunction, lipid accumulation, abnormal inflammatory reaction, excessive reactive oxygen species production, and vascular c...Atherosclerosis occurs as a result of organized processes that include vascular endothelial dysfunction, lipid accumulation, abnormal inflammatory reaction, excessive reactive oxygen species production, and vascular cell proliferation and migration. In patients with atherosclerosis, vascular endothelial dysfunction is commonly observed with the damage of vascular endothelial glycocalyx, which is an extracellular matrix bound to and encapsulating the endothelial cells that line the blood vessel wall. Unhealthy lifestyle choices such as smoking and physical inactivity also induce glycocalyx degradation. Additionally, vascular endothelial glycocalyx can be damaged by various pathological conditions including dehydration, acute infectious disease, trauma, sepsis, acute respiratory distress syndrome, Kawasaki disease, preeclampsia, gestational diabetes mellitus, hypertension, diabetes mellitus, chronic kidney disease, atherosclerosis, stroke, dementia, microvascular angina, acute coronary syndrome, and heart failure. Vascular endothelial glycocalyx has been shown to be important as a physical cytoprotective barrier for vascular endothelial cells and as a regulatory mechanism for intracellular cell signaling. Therefore, vascular endothelial glycocalyx has immense potential in the exploration of novel strategies for the evaluation of beneficial conditions of healthy vasculature.展开更多
Objective： To study the effect of Shengmai injection （生脉注射液, SMI） on vascular endothelial and heart functions in coronary heart disease patients complicated with diabetes mellitus （CHD-DM）. Methods： One hun...Objective： To study the effect of Shengmai injection （生脉注射液, SMI） on vascular endothelial and heart functions in coronary heart disease patients complicated with diabetes mellitus （CHD-DM）. Methods： One hundred and twenty patients with CHD-DM, their diagnosis confirmed by coronary arteriography, were equally randomized into a control group treated with conventional treatment and a treated group treated with conventional treatment plus SMI. The changes in blood levels of nitric oxide （NO）, endothelin-1 （ET-1） and angiotensin Ⅱ （Ang Ⅱ）, as well as endothelium-dependent vascular dilating function and heart function in the patients were observed before treatment and after the 3-week treatment. Results： After being treated with SMI for 3 weeks, in the treated group, blood level of NO was raised significantly from 69.8±33.1 μmol/L to 120.1±50.8μmol/L, and ET-1 was lowered from 70.1±32.1 ng/L to 46.2±21.3 ng/L, respectively （P〈0.01）; that of Ang Ⅱ was lowered from 81.3±24.3 ng/L to 50.2±27.3 ng/L （P〈0.01）; brachial arterial post-congestion blood flow increasing rate was raised from 389.4±26.3% to 459.3±27.8% （P〈0.01）; and the improvement in heart function as seen through the ejection fraction （EF） was increased from 44±5% to 68±6% （P〈0.01）, all the changes being more significant than those in the control group （all P〈0.01）. Conclusion： SMI can improve not only the endothelial function in CHD-DM patients, but also heart contraction significantly.展开更多
Objective： To observe the changes of vascular endothelial functions and general neuroendocrine-immunity （NEI） network under the state of qi-deficiency syndrome induced by excessive idleness and to approach their in...Objective： To observe the changes of vascular endothelial functions and general neuroendocrine-immunity （NEI） network under the state of qi-deficiency syndrome induced by excessive idleness and to approach their internal relevance and illuminate initially the pathophysiological mechanism of vascular lesion induced by excessive idleness. Methods： A total of 100 male Wistar rats were randomly divided into the control group and the qi-deficiency syndrome model group, 50 rats in each group. The qi-deficiency syndrome model was established by feeding the animals with hyper-alimentation diet in combination with restricting movement for 10 weeks. Changes of common chemical signal molecules related to NEI and vascular endothelial functions were measured by the end of the experiment. Furthermore, their internal relevance was analyzed by the method of canonical correlation analysis. Results： The vascular endothelial structure and function were obviously injured in the model group. Compared with the control group, the chemical signal molecules, such as 5-hydroxytryptamine （5-HT）, corticosterone （CORT）, triiodothyronine （T3）, tetraiodothyronine （T4）, angiotensin Ⅱ （Ang Ⅱ）, interleukin-1 （IL-1）, and tumor necrosis factor-α （TNF-α） in peripheral blood of the model group （n=43） were changed significantly （P〈0.05 or P〈0.01）. Canonical correlation analysis showed that vascular endothelial dysfunction was correlated to the changes of these signal molecules in the NEI network. Conclusions： Comfortbased lifestyle induced not only vascular endothelial dysfunction but also an imbalance of the NEI network. Vascular endothelial dysfunction and the imbalanced NEI network interacted with each other, and an imbalance of the NEI network may be the pathophysiologic basis for the genesis and development of vascular endothelial dysfunction, even diseases of the blood vessel.展开更多
Objective To explore the main components and potential mechanisms of Sangqi Qingxuan Liquid in the treatment of arterial vascular endothelial cells(AVECs)injury in hypertension through network pharmacology.Methods Tra...Objective To explore the main components and potential mechanisms of Sangqi Qingxuan Liquid in the treatment of arterial vascular endothelial cells(AVECs)injury in hypertension through network pharmacology.Methods Traditional Chinese Medicine Systems Pharmacology and Analysis Platform(TCMSP)and Traditional Chinese Medicine Integrated Database(TCMID)were used to screen the active components of Sangqi Qingxuan Liquid(SQQX),which met the oral utilization rate and drug similarity criteria.An active component-target network was constructed using Cytoscape 3.6 software.A protein-protein interaction(PPI)network of targets associated with SQQX treatment for hypertension was constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins(STRING)database.The Metascape database was used to perform enrichment analysis of gene ontology biological functions and MSigDB pathway enrichment analysis of proteins in the PPI network.Further analysis of the main components of SQQX was performed using UPLC-MS.Based on the results of network pharmacology,the mechanism of SQQX to improve the injury of AVECs in hypertension was verified through lentiviral transfection by Wnt/β-catenin signaling pathway.AVECs induced by angiotensin II(Ang II)was used to establish a model of endothelial function injury in hypertension.Cell viability,intracellular nitric oxide content,malonaldehyde content,and superoxide dismutase activity were measured to determine the optimal induction conditions.The optimal intervention conditions for SQQX were determined based on cell viability,cellular DNA activity,and the gradient method.The cells were further divided into blank,model,overexpression lentivirus negative control,overexpression lentivirus,overexpression lentivirus+SQQX intervention(2.47 mg/mL,12 h),inhibition lentivirus negative control,inhibition lentivirus,and inhibition lentivirus+SQQX intervention(2.47 mg/mL,12 h)groups.Finally,quantitative real-time PCR and Western blotting were performed to analyze the molecular mechanisms of SQQX in the Wnt/β-catenin signaling pathway.展开更多
OBJECTIVE:To describe a protocol to assess the effects of Traditional Chinese Medicine(TCM)on patients with coronary heart disease(CHD)showing symptoms of phlegm-heat-stasis symptom pattern.METHODS:This is a single-bl...OBJECTIVE:To describe a protocol to assess the effects of Traditional Chinese Medicine(TCM)on patients with coronary heart disease(CHD)showing symptoms of phlegm-heat-stasis symptom pattern.METHODS:This is a single-blind randomized controlled trial that will be conducted in the First Teaching Hospital of Tianjin University of TCM and 60 patients with CHD showing phlegm-heat-stasis symptom pattern will be included.Patients will be randomly divided into either a treatment group(Qingre Huatan formulae+Western Medicine)or to acontrol group(conventional Western Medicine only)for 7-14 d.Primary patient outcomes will be vascular endothelial function and quality of life.Measurement data will be expressed as mean±standard deviation using t-test analysis or repeated-measure variance analysis.Enumeration data will be expressed by cases and percentages,usingχ^(2) analysis,and rank sum test will be used for ranked data.RESULTS:This study further verified the effectiveness and safety of Qingre Huatan formulae for the phlegm-heat-stasis syndrome pattern of CHD on the basis of previous studies on the characteristics of syndromes and medication rules.DISCUSSION:Phlegm-heat-stasis symptom pattern has become a common manifestation in CHD.Standardized Western medications together with TCM have been extensively used in China and have developed into a comprehensive treatment model.Our trial will help formulate recommendations for symptom maintenance and provide clinical evidence for the application of TCM for patients with CHD showing phlegm-heat-stasis symptom pattern.展开更多
Cardiovascular disease is a major cause of mortality in diabetic patients due to the heightened oxidative stress and pro-inflammatory state in vascular tissues.Effective approaches targeting cardiovascular health for ...Cardiovascular disease is a major cause of mortality in diabetic patients due to the heightened oxidative stress and pro-inflammatory state in vascular tissues.Effective approaches targeting cardiovascular health for diabetic patients are urgently needed.Crocodile blood,an emerging dietary supplement,was suggested to have anti-oxidative and anti-inflammatory effects in vitro,which have yet to be proven in animal models.This study thereby aimed to evaluate whether crocodile blood can protect vascular function in diabetic mice against oxidation and inflammation.Diabetic db/db mice and their counterparts db/m+mice were treated daily with crocodile blood soluble fraction(CBSF)or vehicle via oral gavage for 4 weeks before their aortae were harvested for endothelium-dependent relaxation(EDR)quantification using wire myograph,which is a well-established functional study for vascular function indication.Organ culture experiments culturing mouse aortae from C57BL/6 J mice with or without IL-1βand CBSF were done to evaluate the direct effect of CBSF on endothelial function.Reactive oxygen species(ROS)levels in mouse aortae were assessed by dihydroethidium(DHE)staining with inflammatory markers in endothelial cells quantified by quantitative polymerase chain reaction(qPCR).CBSF significantly improved deteriorated EDR in db/db diabetic mice through both diet supplementation and direct culture,with suppression of ROS level in mouse aortae.CBSF also maintained EDR and reduced ROS levels in mouse aortae against the presence of pro-inflammatory IL-1β.Under the pro-inflammatory state induced by IL-1β,gene expressions of inflammatory cytokines were downregulated,while the protective transcripts UCP2 and SIRT6 were upregulated in endothelial cells.Our study suggests a novel beneficial effect of crocodile blood on vascular function in diabetic mice and that supplementation of diet with crocodile blood may act as a complementary approach to protect against vascular diseases through anti-oxidation and anti-inflammation in diabetic patients.展开更多
文摘Background The co-existence of Type 2 diabetes mellitus(T2DM)and hypertension presents a significant clinical challenge due to their synergistic detrimental effects on cardiovascular outcomes.Conventional combination therapies often fall short in comprehensively addressing the intertwined pathophysiologies,particularly vascular endothelial dysfunction.This study aimed to evaluate the efficacy of a novel regimen combining sitagliptin-metformin with nifedipine in managing blood pressure,blood glucose,and vascular function in this high-risk population.Methods 150 patients with T2DM and hypertension were randomly assigned to either the control group(n=75)treated with metformin and nifedipine,or the experimental group(n=75)treated with sitagliptin-metformin compound preparation combined with nifedipine.Changes in blood pressure,blood glucose levels,flow-mediated dilation(FMD)and nitroglycerin-mediated dilation(NMD)of the brachial artery,and serum levels of biomarkers[nitric oxide(NO),endothelin-1(ET-1)]were compared before and after treatment.The incidence of adverse reactions during treatment was also monitored.Results After treatment,both groups showed significant reductions in blood pressure,blood glucose parameters,and ET-1 levels compared to baseline.The experimental group demonstrated significantly lower values in these assessments at the same time points than control group(P<0.05).Conversely,FMD,NMD,and NO levels increased significantly in both groups after treatment,with experimental group showing significantly higher values than control group(P<0.05).There was no statistically significant difference in the incidence of adverse reactions between the two groups(P>0.05).Conclusions The combination of sitagliptinmetformin compound preparation and nifedipine significantly enhanced blood pressure control and blood glucose control and exerted a positive regulatory effect on vascular endothelial function in patients with T2DM and hypertension,demonstrating high clinical value for widespread application.
文摘Background Coronary heart disease with myocardial ischemia is closely associated with vascular endothelial dysfunction,dyslipidemia,and chronic inflammatory response.Atorvastatin,a classic lipid-lowering agent,exhibits pleiotropic effects including plaque stabilization,endothelial function improvement,and anti-inflammatory actions.Nicorandil,as a potassium channel opener with additional nitrate-like effects,directly dilates coronary arteries and improves myocardial microcirculation.Theoretically,the two drugs may synergistically enhance myocardial perfusion and endothelial function through distinct mechanisms.However,clinical evidence regarding the comprehensive impact of their combination on vascular endothelial function,blood lipid profiles,and systemic inflammatory status in patients with coronary heart disease and myocardial ischemia remains insufficient.Further studies are warranted to clarify their synergistic effects and clinical value.Methods A total of 60 patients with coronary heart disease and myocardial ischemia admitted to our hospital from January 2023 to October 2024 were enrolled and randomly divided into combination group and control group using a random number table,with 30 patients in each group.The control group received atorvastatin treatment,while the combination group received nicorandil combined with atorvastatin.After 12 weeks of continuous treatment,the clinical efficacy,vascular endothelial function,blood lipid profiles,inflammatory factors,and adverse reactions were compared between the two groups.Results The total effective rate in the combination group(93.33%)was significantly higher than that in the control group(70.00%)(P<0.05).After treatment,serum endothelin(ET)level was lower in the combination group than in the control group(P<0.05),while nitric oxide(NO)level was higher(P<0.05).In the combination group,the levels of total cholesterol(TC),triglyceride(TG),and low-density lipoprotein cholesterol(LDL-C)were all lower than those in the control group(P<0.05),while high-density lipoprotein cholesterol(HDL-C)level was higher(P<0.05).Levels of C-reactive protein(CRP),interleukin(IL)-8,and IL-6 in the combination group were also lower compared with the control group(P<0.05).No significant difference was observed in the incidence of adverse reactions between the two groups(P>0.05).Conclusions The combination of nicorandil and atorvastatin demonstrated certain short-term therapeutic effects in patients with coronary heart disease and myocardial ischemia.It not only effectively improves vascular endothelial function and regulates lipid metabolism but also suppresses the levels of inflammatory factors,with a favorable safety profile.
基金Supported by General Program of National Natural Science of China:Qigui Slimming Prescription Upgrades the Activating Ability of the Phlegm-Dampness Constitution to Improve Obesity by Regulating the Thermogenesis Axis of Clostridium Enterica-PhytosphingosineSph K/S1P-Ca^(2+) Cycle(No.82374308)National Key Research and Development Program:Development of Dietary Intervention Series for the Elderly with Decreased Functionality(No.2022YFC2010104)National Nonprofit Institute Research Grant for Institute of Basic Theory for Chinese Medicine,CACMS:Methylation of Gut Microbiota-Host DNA Reveals the Mechanism of Promoting PhlegmDampness Constitution to Prevent Metabolic Diseases(No.YZ-202151)。
文摘OBJECTIVE:To evaluate the extent of vascular endothelial dysfunction and preliminary identify serum protein biomarkers associated with obese individuals at risk for cardiovascular disease(CVD).METHODS:Fifteen obese volunteers with the phlegmdampness constitution or balanced constitution were recruited for this study respectively.The clinical baseline data was collected,and the vascular endothelial function was evaluated using the EndoPAT?.Blood samples were collected for the serum proteome analysis.The differences in the serum protein expression levels between the two groups were detected and the protein interaction network analysis,correlation analysis,receiver operating characteristic(ROC)curve analysis,and random forest model investigation were conducted.RESULTS:There were no statistical differences found in the baseline data.For vascular endothelial function,the reactive hyperemia index(RHI)of the phlegm-dampness constitution obese group was significantly lower than that of the balanced constitution obese group(1.46±0.30 vs 2.82±0.78,P<0.0001),indicating vascular endothelial dysfunction.There are 66 differentially expressed serum proteins between the two groups.apolipoprotein A2(Apo A2),angiotensin-converting enzyme 2(ACE-2),interleukin-33(IL-33),and forkhead box P3(FoxP3)showed significant differences and area under curve values of their ROC curves were greater than 0.7 and correlated significantly with RHI.CONCLUSION:Vascular endothelial dysfunction was present in the phlegm-dampness constitution obese group.Thus,alterations in the expression levels of key serum proteins,including Apo A2,ACE-2,IL-33,and Fox P3 could serve as potential biomarkers in the obese population at risk of CVD.
文摘Objective Cardiopulmonary bypass (CPB) and its related ischemia reperfusion injury may cause endothelial cell injury. To study the protective effects of sodium ferulate in vascular endothelial function during CPB by testing the changes of vascular endothelial cell(CEC) ,nitric oxide(NO) and endothelin-1 (ET-1) in children with congenital heart disease. Methods Sixty patients
文摘Objectives To understand the effect of carvedilol on the coronary vascular endothelial function of the patients with coronary heart disease after percutaneous transluminal coronary angioplasty (PTCA). Methods 51cases, having one or more than two branches narrow ( ≥ 70% ) , were diagnosed by coronary angiography. These patients were divided randomly into carvedilol group (n = 28) and control group ( n = 23) who did not take carvedilol. Endothelin (ET) and nitro dioxide (NO) levels of peripheral blood were measured before and after PTCA, before and after two weeks by taking earvedilol. Results Compared with the ET and NO levels before PTCA, ET were markedly increased and NO were decreased after PTCA (p <0. 05) ; compared with the ET and NO levels before taking carvedilol, ET were decreased and NO were increased after two week (p <0.05 ) , but the ET and NO levels of the control group did not change in the period of two weeks observation (p > 0.05). Conclusions Carvedilol may improve the coronary vascular endothelial function after PTCA.
文摘Atherosclerosis occurs as a result of organized processes that include vascular endothelial dysfunction, lipid accumulation, abnormal inflammatory reaction, excessive reactive oxygen species production, and vascular cell proliferation and migration. In patients with atherosclerosis, vascular endothelial dysfunction is commonly observed with the damage of vascular endothelial glycocalyx, which is an extracellular matrix bound to and encapsulating the endothelial cells that line the blood vessel wall. Unhealthy lifestyle choices such as smoking and physical inactivity also induce glycocalyx degradation. Additionally, vascular endothelial glycocalyx can be damaged by various pathological conditions including dehydration, acute infectious disease, trauma, sepsis, acute respiratory distress syndrome, Kawasaki disease, preeclampsia, gestational diabetes mellitus, hypertension, diabetes mellitus, chronic kidney disease, atherosclerosis, stroke, dementia, microvascular angina, acute coronary syndrome, and heart failure. Vascular endothelial glycocalyx has been shown to be important as a physical cytoprotective barrier for vascular endothelial cells and as a regulatory mechanism for intracellular cell signaling. Therefore, vascular endothelial glycocalyx has immense potential in the exploration of novel strategies for the evaluation of beneficial conditions of healthy vasculature.
文摘Objective： To study the effect of Shengmai injection （生脉注射液, SMI） on vascular endothelial and heart functions in coronary heart disease patients complicated with diabetes mellitus （CHD-DM）. Methods： One hundred and twenty patients with CHD-DM, their diagnosis confirmed by coronary arteriography, were equally randomized into a control group treated with conventional treatment and a treated group treated with conventional treatment plus SMI. The changes in blood levels of nitric oxide （NO）, endothelin-1 （ET-1） and angiotensin Ⅱ （Ang Ⅱ）, as well as endothelium-dependent vascular dilating function and heart function in the patients were observed before treatment and after the 3-week treatment. Results： After being treated with SMI for 3 weeks, in the treated group, blood level of NO was raised significantly from 69.8±33.1 μmol/L to 120.1±50.8μmol/L, and ET-1 was lowered from 70.1±32.1 ng/L to 46.2±21.3 ng/L, respectively （P〈0.01）; that of Ang Ⅱ was lowered from 81.3±24.3 ng/L to 50.2±27.3 ng/L （P〈0.01）; brachial arterial post-congestion blood flow increasing rate was raised from 389.4±26.3% to 459.3±27.8% （P〈0.01）; and the improvement in heart function as seen through the ejection fraction （EF） was increased from 44±5% to 68±6% （P〈0.01）, all the changes being more significant than those in the control group （all P〈0.01）. Conclusion： SMI can improve not only the endothelial function in CHD-DM patients, but also heart contraction significantly.
基金Supported by the National Basic Research Program of China (973 Program,No.2005CB523301)the International Science and Technology Cooperation Program(No.2006DFB32460)
文摘Objective： To observe the changes of vascular endothelial functions and general neuroendocrine-immunity （NEI） network under the state of qi-deficiency syndrome induced by excessive idleness and to approach their internal relevance and illuminate initially the pathophysiological mechanism of vascular lesion induced by excessive idleness. Methods： A total of 100 male Wistar rats were randomly divided into the control group and the qi-deficiency syndrome model group, 50 rats in each group. The qi-deficiency syndrome model was established by feeding the animals with hyper-alimentation diet in combination with restricting movement for 10 weeks. Changes of common chemical signal molecules related to NEI and vascular endothelial functions were measured by the end of the experiment. Furthermore, their internal relevance was analyzed by the method of canonical correlation analysis. Results： The vascular endothelial structure and function were obviously injured in the model group. Compared with the control group, the chemical signal molecules, such as 5-hydroxytryptamine （5-HT）, corticosterone （CORT）, triiodothyronine （T3）, tetraiodothyronine （T4）, angiotensin Ⅱ （Ang Ⅱ）, interleukin-1 （IL-1）, and tumor necrosis factor-α （TNF-α） in peripheral blood of the model group （n=43） were changed significantly （P〈0.05 or P〈0.01）. Canonical correlation analysis showed that vascular endothelial dysfunction was correlated to the changes of these signal molecules in the NEI network. Conclusions： Comfortbased lifestyle induced not only vascular endothelial dysfunction but also an imbalance of the NEI network. Vascular endothelial dysfunction and the imbalanced NEI network interacted with each other, and an imbalance of the NEI network may be the pathophysiologic basis for the genesis and development of vascular endothelial dysfunction, even diseases of the blood vessel.
基金Supported by the National Natural Science Foundation of China(No.81774105)Elite Medical Professionals Project of China-Japan Friendship Hospital(No.ZRJY2021-TD03)。
文摘Objective To explore the main components and potential mechanisms of Sangqi Qingxuan Liquid in the treatment of arterial vascular endothelial cells(AVECs)injury in hypertension through network pharmacology.Methods Traditional Chinese Medicine Systems Pharmacology and Analysis Platform(TCMSP)and Traditional Chinese Medicine Integrated Database(TCMID)were used to screen the active components of Sangqi Qingxuan Liquid(SQQX),which met the oral utilization rate and drug similarity criteria.An active component-target network was constructed using Cytoscape 3.6 software.A protein-protein interaction(PPI)network of targets associated with SQQX treatment for hypertension was constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins(STRING)database.The Metascape database was used to perform enrichment analysis of gene ontology biological functions and MSigDB pathway enrichment analysis of proteins in the PPI network.Further analysis of the main components of SQQX was performed using UPLC-MS.Based on the results of network pharmacology,the mechanism of SQQX to improve the injury of AVECs in hypertension was verified through lentiviral transfection by Wnt/β-catenin signaling pathway.AVECs induced by angiotensin II(Ang II)was used to establish a model of endothelial function injury in hypertension.Cell viability,intracellular nitric oxide content,malonaldehyde content,and superoxide dismutase activity were measured to determine the optimal induction conditions.The optimal intervention conditions for SQQX were determined based on cell viability,cellular DNA activity,and the gradient method.The cells were further divided into blank,model,overexpression lentivirus negative control,overexpression lentivirus,overexpression lentivirus+SQQX intervention(2.47 mg/mL,12 h),inhibition lentivirus negative control,inhibition lentivirus,and inhibition lentivirus+SQQX intervention(2.47 mg/mL,12 h)groups.Finally,quantitative real-time PCR and Western blotting were performed to analyze the molecular mechanisms of SQQX in the Wnt/β-catenin signaling pathway.
基金Supported by the National Administration of Traditional Chinese Medicine,Research Project for Practice Development of National Traditional Chinese Medicine Clinical Research Bases:the effects of Traditional Chinese Medicine on Vascular Endothelial Function and Quality of Life in coronary heart disease showing phlegm-heat-stasis symptom pattern(No.JDZX2015003)。
文摘OBJECTIVE:To describe a protocol to assess the effects of Traditional Chinese Medicine(TCM)on patients with coronary heart disease(CHD)showing symptoms of phlegm-heat-stasis symptom pattern.METHODS:This is a single-blind randomized controlled trial that will be conducted in the First Teaching Hospital of Tianjin University of TCM and 60 patients with CHD showing phlegm-heat-stasis symptom pattern will be included.Patients will be randomly divided into either a treatment group(Qingre Huatan formulae+Western Medicine)or to acontrol group(conventional Western Medicine only)for 7-14 d.Primary patient outcomes will be vascular endothelial function and quality of life.Measurement data will be expressed as mean±standard deviation using t-test analysis or repeated-measure variance analysis.Enumeration data will be expressed by cases and percentages,usingχ^(2) analysis,and rank sum test will be used for ranked data.RESULTS:This study further verified the effectiveness and safety of Qingre Huatan formulae for the phlegm-heat-stasis syndrome pattern of CHD on the basis of previous studies on the characteristics of syndromes and medication rules.DISCUSSION:Phlegm-heat-stasis symptom pattern has become a common manifestation in CHD.Standardized Western medications together with TCM have been extensively used in China and have developed into a comprehensive treatment model.Our trial will help formulate recommendations for symptom maintenance and provide clinical evidence for the application of TCM for patients with CHD showing phlegm-heat-stasis symptom pattern.
基金supported by the Hong Kong Research Grants Council Grant ECS[24163117]GRF[14101119]+1 种基金National Natural Science Foundation of China[81970423]SKLA(CUHK).
文摘Cardiovascular disease is a major cause of mortality in diabetic patients due to the heightened oxidative stress and pro-inflammatory state in vascular tissues.Effective approaches targeting cardiovascular health for diabetic patients are urgently needed.Crocodile blood,an emerging dietary supplement,was suggested to have anti-oxidative and anti-inflammatory effects in vitro,which have yet to be proven in animal models.This study thereby aimed to evaluate whether crocodile blood can protect vascular function in diabetic mice against oxidation and inflammation.Diabetic db/db mice and their counterparts db/m+mice were treated daily with crocodile blood soluble fraction(CBSF)or vehicle via oral gavage for 4 weeks before their aortae were harvested for endothelium-dependent relaxation(EDR)quantification using wire myograph,which is a well-established functional study for vascular function indication.Organ culture experiments culturing mouse aortae from C57BL/6 J mice with or without IL-1βand CBSF were done to evaluate the direct effect of CBSF on endothelial function.Reactive oxygen species(ROS)levels in mouse aortae were assessed by dihydroethidium(DHE)staining with inflammatory markers in endothelial cells quantified by quantitative polymerase chain reaction(qPCR).CBSF significantly improved deteriorated EDR in db/db diabetic mice through both diet supplementation and direct culture,with suppression of ROS level in mouse aortae.CBSF also maintained EDR and reduced ROS levels in mouse aortae against the presence of pro-inflammatory IL-1β.Under the pro-inflammatory state induced by IL-1β,gene expressions of inflammatory cytokines were downregulated,while the protective transcripts UCP2 and SIRT6 were upregulated in endothelial cells.Our study suggests a novel beneficial effect of crocodile blood on vascular function in diabetic mice and that supplementation of diet with crocodile blood may act as a complementary approach to protect against vascular diseases through anti-oxidation and anti-inflammation in diabetic patients.
