AIM:To study the production and secretion of corticotropin-releasing factor (CRF) by dendritic cells and the influence of commensal bacteria.METHODS:JAWSⅡ cells (ATCC CRL-11904),a mouse dendritic cell line,were seede...AIM:To study the production and secretion of corticotropin-releasing factor (CRF) by dendritic cells and the influence of commensal bacteria.METHODS:JAWSⅡ cells (ATCC CRL-11904),a mouse dendritic cell line,were seeded into 24-well culture plates and grown for 3 d.Commensal bacterial strains of Clostridium clostrodiiforme (JCM1291),Bacteroides vulgatus (B.vulgatus) (JCM5856),Escherichia coli (JCM1649),or Fusobacterium varium (F.varium) (ATCC8501) were added to the cells except for the control well,and incubated for 2 h.After incubation,we performed enzyme-linked immunosorbent assay for the cultured medium and reverse transcription polymerase chain reaction for the dendritic cells,and compared these values with controls.RESULTS:The level of CRF secretion by control dendritic cells was 40.4±6.2 pg/mL.The CRF levels for cells incubated with F.varium and B.vulgatus were significantly higher than that of the control (P<0.0001).CRF mRNA was present in the control sample without bacteria,and CRF mRNA levels in all samples treated with bacteria were above that of the control sample.F.varium caused the greatest increase in CRF mRNA expression.CONCLUSION:Our results suggest that dendritic cells produce CRF,a process augmented by commensal bacteria.展开更多
对金针菇葡枝霉病的病原物进行了分离培养、鉴定和生物学特性研究。结果认为:引起该病的病原菌为Cladobotryum varium Nees ex Steud。病原菌菌丝生长的适宜温度为10～30℃,最适温度为15～20℃。适宜的pH 值为4.5～8.0,最适pH 值为5.5...对金针菇葡枝霉病的病原物进行了分离培养、鉴定和生物学特性研究。结果认为:引起该病的病原菌为Cladobotryum varium Nees ex Steud。病原菌菌丝生长的适宜温度为10～30℃,最适温度为15～20℃。适宜的pH 值为4.5～8.0,最适pH 值为5.5。温度高、湿度大有利于病害的发生。室内药剂抑菌试验结果表明:质量浓度为0.01 g/L的百菌清和0.5 g/L～1.0 g/L的多菌灵对病菌的菌丝生长有较好的抑菌作用。展开更多
基金Supported by Grants in Aid for Scientific Research (C) from the Japanese Ministry of Education,Culture,Sports,Science and Technology,No. 17590679
文摘AIM:To study the production and secretion of corticotropin-releasing factor (CRF) by dendritic cells and the influence of commensal bacteria.METHODS:JAWSⅡ cells (ATCC CRL-11904),a mouse dendritic cell line,were seeded into 24-well culture plates and grown for 3 d.Commensal bacterial strains of Clostridium clostrodiiforme (JCM1291),Bacteroides vulgatus (B.vulgatus) (JCM5856),Escherichia coli (JCM1649),or Fusobacterium varium (F.varium) (ATCC8501) were added to the cells except for the control well,and incubated for 2 h.After incubation,we performed enzyme-linked immunosorbent assay for the cultured medium and reverse transcription polymerase chain reaction for the dendritic cells,and compared these values with controls.RESULTS:The level of CRF secretion by control dendritic cells was 40.4±6.2 pg/mL.The CRF levels for cells incubated with F.varium and B.vulgatus were significantly higher than that of the control (P<0.0001).CRF mRNA was present in the control sample without bacteria,and CRF mRNA levels in all samples treated with bacteria were above that of the control sample.F.varium caused the greatest increase in CRF mRNA expression.CONCLUSION:Our results suggest that dendritic cells produce CRF,a process augmented by commensal bacteria.
文摘对金针菇葡枝霉病的病原物进行了分离培养、鉴定和生物学特性研究。结果认为:引起该病的病原菌为Cladobotryum varium Nees ex Steud。病原菌菌丝生长的适宜温度为10～30℃,最适温度为15～20℃。适宜的pH 值为4.5～8.0,最适pH 值为5.5。温度高、湿度大有利于病害的发生。室内药剂抑菌试验结果表明:质量浓度为0.01 g/L的百菌清和0.5 g/L～1.0 g/L的多菌灵对病菌的菌丝生长有较好的抑菌作用。
