Heteroconchia,a widespread and abundant aquatic invertebrate,is an important clade of bivalve mollusks.The relationship between the three branches of Heteroconchia,Palaeoheterodonta,Archiheterodonta,and Euheterodonta ...Heteroconchia,a widespread and abundant aquatic invertebrate,is an important clade of bivalve mollusks.The relationship between the three branches of Heteroconchia,Palaeoheterodonta,Archiheterodonta,and Euheterodonta has become a main controversy in molecular studies of the relationships between bivalves.In the present study,we assembled the complete mitochondrial genomes of Tapes dorsatus(Veneridae)and Cardita variegata(Carditidae)using high-throughput sequencing.C.variegata is the first mitochondrial genome belonging to the family Carditidae to be reported.We used 12 protein coding genes(excluding atp8)from the complete mitochondrial genomes of 146 species to recover the internal relationships of Heteroconchia.Our results support the traditional view of early branching of Palaeoheterodonta and the recovery of the monophyly of Palaeoheterodonta,Anomalodesmata,Imparidentia.Rearrangement analysis show that gene arrangement within Venerida was highly variable.Time-calibrated phylogenetic studies based on a relaxed molecular clock model suggested that Veneridae originated approximately 337.62 million years ago(Ma)and split into two major clades,whereas Carditidae originated approximately 510.09 Ma.Our results provide evidence of the internal relationships of Heteroconchia.展开更多
In the present study,17 flavonoids,including 9 prenylated isoflavones,4 isoflavones,3 flavanones and 1 flavone,were isolated from the twigs and leaves of Erythrina variegata.By NMR spectroscopic means and comparision ...In the present study,17 flavonoids,including 9 prenylated isoflavones,4 isoflavones,3 flavanones and 1 flavone,were isolated from the twigs and leaves of Erythrina variegata.By NMR spectroscopic means and comparision with the data in the leteratures,their structures were elucidated to be euchrenone b10(1),senegalensin(2),erythrinin C(3),scandenone(4),osajin(5),alpinum isoflavone(6),isoerysenegalensein E(7),erysenegalensein E(8),diprenylgenistein(9),abyssinone V(10),abyssinone V 4′-methyl ether(11),4′,7-dihydroxyflavanone(12),3′,5,7-trihydroxy-4′-methoxyisoflavone(13),2′,4′,5,7-tetrahydroxyisoflavone(14),genistein(15),daidzein(16),and apigenin(17).Except for compounds 1,4-7,and 9-10,other compounds were isolated from E.variegata for the first time.展开更多
Fourteen unprecedented artificial Erythrina alkaloids were isolated from the Erythrina variegata,E.crista-galli and E.arborescens(Fabaceae).The structures of these alkaloids were determined by spectroscopic analyses.T...Fourteen unprecedented artificial Erythrina alkaloids were isolated from the Erythrina variegata,E.crista-galli and E.arborescens(Fabaceae).The structures of these alkaloids were determined by spectroscopic analyses.Their possible forma-tions were proposed.All isolated compounds showed no cytotoxicity and hypoglycemic activity at cell screening bioassay.展开更多
Background:Ceropegia variegata Decne.is a plant traditionally used in medicine,but its full chemical composition and biological potential remain largely unexplored.Objective:This study aimed to identify the bioactive ...Background:Ceropegia variegata Decne.is a plant traditionally used in medicine,but its full chemical composition and biological potential remain largely unexplored.Objective:This study aimed to identify the bioactive compounds in C.variegata stem extracts and evaluate their antioxidant and antimicrobial activities.Methods:Plant stems were sequentially extracted with solvents of increasing polarity.The extracts were analyzed for total phenolic and flavonoid content.Their antioxidant activity was tested by DPPH,FRAPand phosphomolyb-denum assays.Antimicrobial activity was tested against various bacteria and fungi.Liquid chromatography-mass spectrometry(LC-MS)analysis was used to identify compounds in the methanolic extract.Results:The extracts contained a variety of secondary metabolites,with the ethanol extract being richest in phenolics and the methanol extract in flavonoids.The methanolic extract showed the highest antioxidant activity among the tested extracts,while the chloroform extract showed the highest antimicrobial potency.LC-MS analysis revealed 35 compounds,including phenols,flavonoids,alkaloids,terpenes,glycosides,and fatty acids,which likely contribute to the plant’s biological activities.Conclusion:C.variegata stems are a valuable source of bioactive compounds with significant antioxidant and antimicrobial properties.These findings support the plant’s traditional use and suggest its potential for developing natural health products.Further research is needed to isolate and characterize specific bioactive compounds and their mechanisms of action.展开更多
Field insect population survey indicated that the principal natural predation enemies of cotton bollworm ( Helicoverpa armigera Hubner ) were Eringonidium graminicola Sundevall, Adonia variegata Goeze, Nabis sinoferus...Field insect population survey indicated that the principal natural predation enemies of cotton bollworm ( Helicoverpa armigera Hubner ) were Eringonidium graminicola Sundevall, Adonia variegata Goeze, Nabis sinoferus Hsiao.The results from experiments showed that the amount of prey consumed of these principal natural enemies increased when the density of prey rised,and decreased when the density of natural enemies increased. The predation function response fitted Holling’s predation response equationⅡ,and could be fit with the Disk展开更多
基金Supported by the Research and Development Program of Shandong Province,China(Major Science and Technology Innovation Project)(No.2021CXGC011306)the MNR Key Laboratory of Eco-Environmental Science and Technology,China(No.MEEST-2021-05)+2 种基金the Natural Science Foundation of Shandong Province(No.ZR2020MD002)the Doctoral Science Research Foundation of Yantai University(Nos.SM15B01,SM19B70,SM19B28)the“Double-Hundred Action”of Yantai City(No.2320004-SM20RC02)。
文摘Heteroconchia,a widespread and abundant aquatic invertebrate,is an important clade of bivalve mollusks.The relationship between the three branches of Heteroconchia,Palaeoheterodonta,Archiheterodonta,and Euheterodonta has become a main controversy in molecular studies of the relationships between bivalves.In the present study,we assembled the complete mitochondrial genomes of Tapes dorsatus(Veneridae)and Cardita variegata(Carditidae)using high-throughput sequencing.C.variegata is the first mitochondrial genome belonging to the family Carditidae to be reported.We used 12 protein coding genes(excluding atp8)from the complete mitochondrial genomes of 146 species to recover the internal relationships of Heteroconchia.Our results support the traditional view of early branching of Palaeoheterodonta and the recovery of the monophyly of Palaeoheterodonta,Anomalodesmata,Imparidentia.Rearrangement analysis show that gene arrangement within Venerida was highly variable.Time-calibrated phylogenetic studies based on a relaxed molecular clock model suggested that Veneridae originated approximately 337.62 million years ago(Ma)and split into two major clades,whereas Carditidae originated approximately 510.09 Ma.Our results provide evidence of the internal relationships of Heteroconchia.
基金National Natural Science Foundation of China(Grant No.32060099).
文摘In the present study,17 flavonoids,including 9 prenylated isoflavones,4 isoflavones,3 flavanones and 1 flavone,were isolated from the twigs and leaves of Erythrina variegata.By NMR spectroscopic means and comparision with the data in the leteratures,their structures were elucidated to be euchrenone b10(1),senegalensin(2),erythrinin C(3),scandenone(4),osajin(5),alpinum isoflavone(6),isoerysenegalensein E(7),erysenegalensein E(8),diprenylgenistein(9),abyssinone V(10),abyssinone V 4′-methyl ether(11),4′,7-dihydroxyflavanone(12),3′,5,7-trihydroxy-4′-methoxyisoflavone(13),2′,4′,5,7-tetrahydroxyisoflavone(14),genistein(15),daidzein(16),and apigenin(17).Except for compounds 1,4-7,and 9-10,other compounds were isolated from E.variegata for the first time.
基金supported in part by the National Natural Science Foundation of China(31872677).
文摘Fourteen unprecedented artificial Erythrina alkaloids were isolated from the Erythrina variegata,E.crista-galli and E.arborescens(Fabaceae).The structures of these alkaloids were determined by spectroscopic analyses.Their possible forma-tions were proposed.All isolated compounds showed no cytotoxicity and hypoglycemic activity at cell screening bioassay.
文摘Background:Ceropegia variegata Decne.is a plant traditionally used in medicine,but its full chemical composition and biological potential remain largely unexplored.Objective:This study aimed to identify the bioactive compounds in C.variegata stem extracts and evaluate their antioxidant and antimicrobial activities.Methods:Plant stems were sequentially extracted with solvents of increasing polarity.The extracts were analyzed for total phenolic and flavonoid content.Their antioxidant activity was tested by DPPH,FRAPand phosphomolyb-denum assays.Antimicrobial activity was tested against various bacteria and fungi.Liquid chromatography-mass spectrometry(LC-MS)analysis was used to identify compounds in the methanolic extract.Results:The extracts contained a variety of secondary metabolites,with the ethanol extract being richest in phenolics and the methanol extract in flavonoids.The methanolic extract showed the highest antioxidant activity among the tested extracts,while the chloroform extract showed the highest antimicrobial potency.LC-MS analysis revealed 35 compounds,including phenols,flavonoids,alkaloids,terpenes,glycosides,and fatty acids,which likely contribute to the plant’s biological activities.Conclusion:C.variegata stems are a valuable source of bioactive compounds with significant antioxidant and antimicrobial properties.These findings support the plant’s traditional use and suggest its potential for developing natural health products.Further research is needed to isolate and characterize specific bioactive compounds and their mechanisms of action.
文摘Field insect population survey indicated that the principal natural predation enemies of cotton bollworm ( Helicoverpa armigera Hubner ) were Eringonidium graminicola Sundevall, Adonia variegata Goeze, Nabis sinoferus Hsiao.The results from experiments showed that the amount of prey consumed of these principal natural enemies increased when the density of prey rised,and decreased when the density of natural enemies increased. The predation function response fitted Holling’s predation response equationⅡ,and could be fit with the Disk
