【目的】GRAM(Glucosyltransferases,Rab-like GTPase activators and Myotubularins)是普遍存在于动、植物蛋白中的结构域,在植物生长发育及响应逆境胁迫等过程中发挥着重要功能。在马铃薯全基因组中鉴定GRAM基因家族成员,分析马铃薯G...【目的】GRAM(Glucosyltransferases,Rab-like GTPase activators and Myotubularins)是普遍存在于动、植物蛋白中的结构域,在植物生长发育及响应逆境胁迫等过程中发挥着重要功能。在马铃薯全基因组中鉴定GRAM基因家族成员,分析马铃薯GRAM家族基因在盐胁迫下的表达模式,探究GRAM家族在马铃薯盐胁迫过程中的作用。【方法】采用生物信息学方法鉴定马铃薯中GRAM家族成员,并对蛋白理化性质、染色体定位、亚细胞定位、基因结构、motif及共线性等方面进行分析。利用转录组测序和荧光定量PCR(RT-qPCR)对该家族成员在盐胁迫下的表达模式进行研究。【结果】在马铃薯中共鉴定到26个GRAM家族基因,不均匀地分布于7条染色体上;理化性质分析显示StGRAM全部为亲水性蛋白,大部分为碱性蛋白;亚细胞定位预测StGRAM蛋白大部分存在于叶绿体和细胞核;根据系统进化分析可将StGRAM家族分为3个亚族,同一亚族成员具有相似的基因结构及motif分布;通过马铃薯物种内共线性分析发现StGRAM仅有一对同源基因,物种间共线性显示StGRAM在水稻和拟南芥中分别存在5对和3对同源基因;在StGRAM基因启动子区发现大量的激素响应元件和逆境胁迫响应元件;转录组测序分析和RT-qPCR分析显示,StGRAM基因受盐胁迫的诱导表达,可能参与了马铃薯对盐胁迫的响应过程,StGRAM25基因可能对中性盐和碱性盐有不同的响应模式。【结论】StGRAM基因家族在马铃薯盐胁迫响应和信号转导过程中发挥着重要作用。展开更多
随着能源行业的快速发展和技术革新,大量的专业术语和表达方式不断更新,新词不断涌现。然而,传统的新词发现方法通常依赖于词典或规则,且难以高效率地处理和更新大量的专业术语,特别是在快速变化的能源领域。因此,结合能源领域文本数据...随着能源行业的快速发展和技术革新,大量的专业术语和表达方式不断更新,新词不断涌现。然而,传统的新词发现方法通常依赖于词典或规则,且难以高效率地处理和更新大量的专业术语,特别是在快速变化的能源领域。因此,结合能源领域文本数据特性,提出了一种融合N-Gram和多重注意力机制的能源领域新词发现方法(new word discovery method in the energy field combining N-Gram and multiple attention mechanism, ENFM)。该方法首先利用N-Gram模型对能源领域的文本数据进行初步处理,通过统计和分析词频来生成新词候选列表。随后,引入融合多重注意力机制的ERNIE-BiLSTM-CRF模型,以进一步提升新词发现的准确性和效率。与传统的新词发现技术相比,在新词的准确识别和整体效率上均有显著提升,将其于能源领域政策文本数据集,准确率、召回率和F1分别为95.71%、95.56%、95.63%。实验结果表明,该方法能够准确地在能源领域的大量文本数据中识别新词,有效识别出能源领域特有的词汇和表达方式,显著提高了中文分词任务中对能源领域专业术语的识别能力。展开更多
The integrated systems of unmanned surface vehicles(USVs) and remotely operated vehicles(ROVs) have been extensively applied in marine exploration and seabed coverage. However, the simultaneous navigation of USV-ROV s...The integrated systems of unmanned surface vehicles(USVs) and remotely operated vehicles(ROVs) have been extensively applied in marine exploration and seabed coverage. However, the simultaneous navigation of USV-ROV systems is frequently limited by strong disturbances induced by waves or currents. This paper develops a novel rigidflexible coupling multibody dynamic model that incorporates disturbances of variable-length marine cables with geometrically nonlinear motion. A hybrid Lagrangian-Eulerian absolute nodal coordinate formulation(ANCF) element is developed to accurately model subsea cables which undergo significant overall motion, substantial deformation,and mass flow during the deployment of underwater equipment. Furthermore, the governing equations of the coupled USV-umbilical-ROV system are derived, considering wave-induced forces and current disturbances. A numerical solver based on the Newmark-beta method is proposed, along with an adaptive meshing technique near the release point. After validating three experimental cases, the cable disturbances at both the USV and ROV ends—caused by ocean currents, heave motion, and simultaneous navigation—are comprehensively compared and evaluated. Finally,it is demonstrated that a PD controller with disturbance compensation can enhance the simultaneous navigation performance of USV-ROV systems.展开更多
Introduction: Bacterial skin and soft tissue infections (SSTIs) are a cause of frequent inpatient and outpatient care visits whose causative agents are associated with a high antimicrobial resistance burden. For insig...Introduction: Bacterial skin and soft tissue infections (SSTIs) are a cause of frequent inpatient and outpatient care visits whose causative agents are associated with a high antimicrobial resistance burden. For insights on antimicrobial susceptibilities in a rural setting, we examined specimens from suspected SSTIs from two public health facilities in Kenya. We additionally assessed antibiotic use, appropriateness of empiric therapy and risk factors for SSTI. Methodology: Between 2021 and 2023, 265 patients at Kisii and Nyamira County Referral hospitals were enrolled. Wound swabs/aspirates were collected and processed following standard microbiological procedures. Identification and antimicrobial susceptibility were performed using the VITEK 2 Compact platform. Demographic, clinical, and microbiological data were analyzed with R Statistical software. Results: S. aureus was isolated in 16.2% (43/265) of patients with a methicillin resistance (MRSA) proportion of 14% (6/43). While 13/15 drugs elicited susceptibilities ranging from 84% - 100%, penicillin (16%) and trimethoprim-sulfamethoxazole [TMP-SXT] (23%) yielded the lowest susceptibilities. Escherichia coli (n = 33), Klebsiella pneumoniae (n = 8), Pseudomonas aeruginosa (n = 8), and Citrobacter species (n = 4) were the most commonly isolated gram-negative species. Gram-negative strains showed high susceptibilities to most of the tested drugs (71% - 100%) with the exception of ampicillin (18%), TMP-SXT (33%), and first and second generation cephalosporins. Conclusions: The low MRSA prevalence and generally high antibiotic susceptibilities for S. aureus and gram-negative bacteria present opportunities for antibiotic stewardship in the study setting. Diminished susceptibilities against penicillin/ampicillin and TMP-SXT accord with prevailing local data and add a layer of evidence for their cautious empiric use.展开更多
文摘【目的】GRAM(Glucosyltransferases,Rab-like GTPase activators and Myotubularins)是普遍存在于动、植物蛋白中的结构域,在植物生长发育及响应逆境胁迫等过程中发挥着重要功能。在马铃薯全基因组中鉴定GRAM基因家族成员,分析马铃薯GRAM家族基因在盐胁迫下的表达模式,探究GRAM家族在马铃薯盐胁迫过程中的作用。【方法】采用生物信息学方法鉴定马铃薯中GRAM家族成员,并对蛋白理化性质、染色体定位、亚细胞定位、基因结构、motif及共线性等方面进行分析。利用转录组测序和荧光定量PCR(RT-qPCR)对该家族成员在盐胁迫下的表达模式进行研究。【结果】在马铃薯中共鉴定到26个GRAM家族基因,不均匀地分布于7条染色体上;理化性质分析显示StGRAM全部为亲水性蛋白,大部分为碱性蛋白;亚细胞定位预测StGRAM蛋白大部分存在于叶绿体和细胞核;根据系统进化分析可将StGRAM家族分为3个亚族,同一亚族成员具有相似的基因结构及motif分布;通过马铃薯物种内共线性分析发现StGRAM仅有一对同源基因,物种间共线性显示StGRAM在水稻和拟南芥中分别存在5对和3对同源基因;在StGRAM基因启动子区发现大量的激素响应元件和逆境胁迫响应元件;转录组测序分析和RT-qPCR分析显示,StGRAM基因受盐胁迫的诱导表达,可能参与了马铃薯对盐胁迫的响应过程,StGRAM25基因可能对中性盐和碱性盐有不同的响应模式。【结论】StGRAM基因家族在马铃薯盐胁迫响应和信号转导过程中发挥着重要作用。
文摘随着能源行业的快速发展和技术革新,大量的专业术语和表达方式不断更新,新词不断涌现。然而,传统的新词发现方法通常依赖于词典或规则,且难以高效率地处理和更新大量的专业术语,特别是在快速变化的能源领域。因此,结合能源领域文本数据特性,提出了一种融合N-Gram和多重注意力机制的能源领域新词发现方法(new word discovery method in the energy field combining N-Gram and multiple attention mechanism, ENFM)。该方法首先利用N-Gram模型对能源领域的文本数据进行初步处理,通过统计和分析词频来生成新词候选列表。随后,引入融合多重注意力机制的ERNIE-BiLSTM-CRF模型,以进一步提升新词发现的准确性和效率。与传统的新词发现技术相比,在新词的准确识别和整体效率上均有显著提升,将其于能源领域政策文本数据集,准确率、召回率和F1分别为95.71%、95.56%、95.63%。实验结果表明,该方法能够准确地在能源领域的大量文本数据中识别新词,有效识别出能源领域特有的词汇和表达方式,显著提高了中文分词任务中对能源领域专业术语的识别能力。
基金financially supported in part by the General Program of the National Natural Science Foundation of China (Grant No.12272221)the State Key Laboratory of Ocean Engineering (Shanghai Jiao Tong University)(Grant No. GKZD010087)。
文摘The integrated systems of unmanned surface vehicles(USVs) and remotely operated vehicles(ROVs) have been extensively applied in marine exploration and seabed coverage. However, the simultaneous navigation of USV-ROV systems is frequently limited by strong disturbances induced by waves or currents. This paper develops a novel rigidflexible coupling multibody dynamic model that incorporates disturbances of variable-length marine cables with geometrically nonlinear motion. A hybrid Lagrangian-Eulerian absolute nodal coordinate formulation(ANCF) element is developed to accurately model subsea cables which undergo significant overall motion, substantial deformation,and mass flow during the deployment of underwater equipment. Furthermore, the governing equations of the coupled USV-umbilical-ROV system are derived, considering wave-induced forces and current disturbances. A numerical solver based on the Newmark-beta method is proposed, along with an adaptive meshing technique near the release point. After validating three experimental cases, the cable disturbances at both the USV and ROV ends—caused by ocean currents, heave motion, and simultaneous navigation—are comprehensively compared and evaluated. Finally,it is demonstrated that a PD controller with disturbance compensation can enhance the simultaneous navigation performance of USV-ROV systems.
文摘Introduction: Bacterial skin and soft tissue infections (SSTIs) are a cause of frequent inpatient and outpatient care visits whose causative agents are associated with a high antimicrobial resistance burden. For insights on antimicrobial susceptibilities in a rural setting, we examined specimens from suspected SSTIs from two public health facilities in Kenya. We additionally assessed antibiotic use, appropriateness of empiric therapy and risk factors for SSTI. Methodology: Between 2021 and 2023, 265 patients at Kisii and Nyamira County Referral hospitals were enrolled. Wound swabs/aspirates were collected and processed following standard microbiological procedures. Identification and antimicrobial susceptibility were performed using the VITEK 2 Compact platform. Demographic, clinical, and microbiological data were analyzed with R Statistical software. Results: S. aureus was isolated in 16.2% (43/265) of patients with a methicillin resistance (MRSA) proportion of 14% (6/43). While 13/15 drugs elicited susceptibilities ranging from 84% - 100%, penicillin (16%) and trimethoprim-sulfamethoxazole [TMP-SXT] (23%) yielded the lowest susceptibilities. Escherichia coli (n = 33), Klebsiella pneumoniae (n = 8), Pseudomonas aeruginosa (n = 8), and Citrobacter species (n = 4) were the most commonly isolated gram-negative species. Gram-negative strains showed high susceptibilities to most of the tested drugs (71% - 100%) with the exception of ampicillin (18%), TMP-SXT (33%), and first and second generation cephalosporins. Conclusions: The low MRSA prevalence and generally high antibiotic susceptibilities for S. aureus and gram-negative bacteria present opportunities for antibiotic stewardship in the study setting. Diminished susceptibilities against penicillin/ampicillin and TMP-SXT accord with prevailing local data and add a layer of evidence for their cautious empiric use.
文摘目的了解耐碳青霉烯类肺炎克雷伯菌的分布情况及其耐药性,为预防、控制和治疗相关感染提供科学依据。方法使用微生物统计软件Whonet 5.6分析2015年1月—2022年12月某院微生物实验室分离出的耐碳青霉烯类肺炎克雷伯菌患者信息、科室分布、标本种类和药敏试验,使用SPSS 22.0软件进行统计学分析。结果共检出耐碳青霉烯类肺炎克雷伯菌380株,检出率呈逐年波动上升;男性患者(75.26%,286/380)多于女性(24.74%,94/380),多数患者年龄大于45岁(86.58%,329/380)。科室分布,重症监护病房(intensive care unit,ICU)耐碳青霉烯类肺炎克雷伯菌检出最多(76.84%,292/380),不同年份该菌的检出率整体呈上升趋势(χ_(趋势)^(2)=44.151,P<0.001);标本分布,呼吸道标本检出耐碳青霉烯类肺炎克雷伯菌最多(56.58%,215/380);耐碳青霉烯类肺炎克雷伯菌对常见抗菌药物呈现出广泛耐药性,仅多黏菌素和替加环素耐药率较低。结论耐碳青霉烯类肺炎克雷伯菌在ICU患者中检出率高,细菌耐药率高,应制定合理防控措施,避免耐药菌株的产生和传播。
