The Lateral Organ Boundaries Domain(LBD)genes encode highly conserved plant-specific LOB domain proteins which regulate growth and development in various species.However,members of the LBD gene family have yet to be i...The Lateral Organ Boundaries Domain(LBD)genes encode highly conserved plant-specific LOB domain proteins which regulate growth and development in various species.However,members of the LBD gene family have yet to be identified in Brassica rapa var.rapa.In the present study,fifty-nine LBD genes were identified and distributed on 10 chromosomes.The BrrLBD proteins are predicted to encode hydrophobic polypeptides between 118 and 394 amino acids in length and with molecular weights ranging from 13.31 to 44.24 kDa;the theoretical pi for these proteins varies from 4.83 to 9.68.There were 17 paralogous gene pairs in the BrrLBD family,suggesting that the amplification of the BrrLBD gene family involved largescale gene duplication events.Members of the BrrLBD family were divided into 7 subclades(class I a to e,class II a and b).Analysis of gene structure and conserved domains revealed that most BrrLBD genes of the same subclade had similar gene structures and protein motifs.The expression profiles of 59 BrrLBD genes were determined through Quantitative Real-time fluorescent PCR(qRT-PCR).Most BrrLBD genes in the same subclade had similar gene expression profiles.However,the expression patterns of 7 genes differed from their duplicates,indicating that although the gene function of most BrrLBD genes has been conserved,some BrrLBD genes may have undergone evolutionary change.展开更多
本研究以新疆紫色芜菁与绿色芜菁(Brassica rapa var. rapa)为研究对象,结合超高效液相色谱(UPLC)和串联质(MS/MS)技术,对不同皮色芜菁果肉、果皮中花青苷进行分析鉴定。紫色芜菁与绿色芜菁中含有14种相同的花青苷,不同芜菁品种不同部位...本研究以新疆紫色芜菁与绿色芜菁(Brassica rapa var. rapa)为研究对象,结合超高效液相色谱(UPLC)和串联质(MS/MS)技术,对不同皮色芜菁果肉、果皮中花青苷进行分析鉴定。紫色芜菁与绿色芜菁中含有14种相同的花青苷,不同芜菁品种不同部位,花青苷含量不一致,表皮中含有的花青苷种类较多,果肉中含有的花青苷种类较少。不同皮色的芜菁品种在果肉中均含松香花青素O-己糖苷与松香苷3-O-葡萄糖苷。不同品种芜菁表皮中含有差异显著花青苷代谢物数目为8个,果肉中含有差异显著花青苷代谢物数目为5个;紫色芜菁果肉与绿色芜菁表皮花青素代谢物的相关系数为0.989 7;紫色芜菁表皮与绿色芜菁表皮花青素代谢物的相关系数为0.927 3。本研究完成花青苷代谢物的鉴定分析,明确紫色芜菁块根中花青素的分布,揭示新疆芜菁中花青苷类物质的组成差异与积累特性,这为揭示新疆芜菁中花青苷的合成与积累的分子机制和培育富含花青苷的芜菁新品种提供一定的理论基础。展开更多
In higher plants,sugars(mainly sucrose)are produced by photosynthetically assimilated carbon in mesophyll cells of leaves and translocated to heterotrophic organs to ensure plant growth and devel-opment.Sucrose transp...In higher plants,sugars(mainly sucrose)are produced by photosynthetically assimilated carbon in mesophyll cells of leaves and translocated to heterotrophic organs to ensure plant growth and devel-opment.Sucrose transporters,or sucrose carriers(SUCs),play an important role in the long-distance transportation of sucrose from source organs to sink organs,thereby affecting crop yield and quality.The identification,characterization,and molecular function analysis of sucrose transporter genes have been reported for monocot and dicot plants.However,no relevant study has been reported on sucrose transporter genes in Brassica rapa vat.rapa,a cruciferous root crop used mainly as vegetables and fodder.We identified and cloned 12 sucrose transporter genes from turnips,named BrrSUC1.1 to BrrSUCB.2 according to the SUC gene sequences of B.rapa pekinensis.We constructed a phylogenetic tree and analyzed conserved motifs for all 12 sucrose transporter genes identified.Real-time quantitative poly-merase chain reaction was conducted to understand the expression levels of SUC genes in different tissues and developmental phases of the turnip.These findings add to our understanding of the genetics and physiology of sugar transport during taproot formation in turnips.展开更多
基金This study was supported by the Major Program of National Natural Science Foundation of China,China(31590820 and 31590823)the National Natural Science Foundation of China,China(41771123 and 31400244)the Natural Science Foundation of Yunnan Province(2017FB050).
文摘The Lateral Organ Boundaries Domain(LBD)genes encode highly conserved plant-specific LOB domain proteins which regulate growth and development in various species.However,members of the LBD gene family have yet to be identified in Brassica rapa var.rapa.In the present study,fifty-nine LBD genes were identified and distributed on 10 chromosomes.The BrrLBD proteins are predicted to encode hydrophobic polypeptides between 118 and 394 amino acids in length and with molecular weights ranging from 13.31 to 44.24 kDa;the theoretical pi for these proteins varies from 4.83 to 9.68.There were 17 paralogous gene pairs in the BrrLBD family,suggesting that the amplification of the BrrLBD gene family involved largescale gene duplication events.Members of the BrrLBD family were divided into 7 subclades(class I a to e,class II a and b).Analysis of gene structure and conserved domains revealed that most BrrLBD genes of the same subclade had similar gene structures and protein motifs.The expression profiles of 59 BrrLBD genes were determined through Quantitative Real-time fluorescent PCR(qRT-PCR).Most BrrLBD genes in the same subclade had similar gene expression profiles.However,the expression patterns of 7 genes differed from their duplicates,indicating that although the gene function of most BrrLBD genes has been conserved,some BrrLBD genes may have undergone evolutionary change.
基金supported by Major Program of National Natural Science Foundation of China (31590820,31590823)
文摘In higher plants,sugars(mainly sucrose)are produced by photosynthetically assimilated carbon in mesophyll cells of leaves and translocated to heterotrophic organs to ensure plant growth and devel-opment.Sucrose transporters,or sucrose carriers(SUCs),play an important role in the long-distance transportation of sucrose from source organs to sink organs,thereby affecting crop yield and quality.The identification,characterization,and molecular function analysis of sucrose transporter genes have been reported for monocot and dicot plants.However,no relevant study has been reported on sucrose transporter genes in Brassica rapa vat.rapa,a cruciferous root crop used mainly as vegetables and fodder.We identified and cloned 12 sucrose transporter genes from turnips,named BrrSUC1.1 to BrrSUCB.2 according to the SUC gene sequences of B.rapa pekinensis.We constructed a phylogenetic tree and analyzed conserved motifs for all 12 sucrose transporter genes identified.Real-time quantitative poly-merase chain reaction was conducted to understand the expression levels of SUC genes in different tissues and developmental phases of the turnip.These findings add to our understanding of the genetics and physiology of sugar transport during taproot formation in turnips.
