Interleukin-8 homolog (vIL-8) is a chemokine encoded by the genome of Marek's disease virus. Chicken IL-8 (cIL8) is an important chemokine of chickens which plays a role in antiviral activity. To explore the relat...Interleukin-8 homolog (vIL-8) is a chemokine encoded by the genome of Marek's disease virus. Chicken IL-8 (cIL8) is an important chemokine of chickens which plays a role in antiviral activity. To explore the relationship between vIL-8 and cIL8 can help to discern the function of vIL-8. In this study, the amino acid sequences of vIL-8 and cIL8 were aligned. The cIL8 gene was expressed in E. coli and the cIL8 fusion protein was obtained, after induction with IPTG. The protein was separated by SDS-PAGE and the band of interest was excised and minced for mouse immunization. An immunofluorescence test was used to detect vIL-8 expression in insect cells. The results showed that both vIL-8 and cIL8 were typical CXC chemokines in structure, and they had similar key amino acids, known to be important for receptor binding. The result of the immunofluorescence test showed that the mouse anti-cIL8 serum could react with vIL-8 expressed by insect cells. Therefore, vIL-8 shares common antigenic determinants with cIL8, and they may have a common receptor. This feature of vIL-8 suggests that it may participate in the immune evasion of the virus.展开更多
研究旨在通过对辽宁省冰雹高发地区的一次典型强雹暴过程进行中尺度特征分析,寻找致雹物理参量特征及雷达临近预警指标,以期为冰雹短临预报预警提供参考。利用常规气象观测资料、葵花卫星、多普勒天气雷达、NCEP/FNL等资料,对发生在辽...研究旨在通过对辽宁省冰雹高发地区的一次典型强雹暴过程进行中尺度特征分析,寻找致雹物理参量特征及雷达临近预警指标,以期为冰雹短临预报预警提供参考。利用常规气象观测资料、葵花卫星、多普勒天气雷达、NCEP/FNL等资料,对发生在辽宁建平地区一次强冰雹天气的天气形势及中尺度特征进行了详细分析。结果表明:此次冰雹过程发生在较强的风切变环境场中,850 h Pa与500 h Pa风向切变>90°,850 h Pa与500 h Pa之间的温度差>26℃,大气层结不稳定;冰雹发生在强对流云团发展至成熟阶段初期,在可见光云图上可以看出明显的纹理和暗影,TBB较长时间维持在-50℃以下,冰雹发生后,TBB梯度值迅速减小;强降雹超级单体呈现弓状,除了具有入流边界、回波悬垂等雷达特征外,还具有高悬的反射率因子核心,即>55 d BZ高度伸展到-20℃以上,以及VIL>60 kg/m^2,VIL密度>4 g/m^3,深厚持久的中气旋维持超级单体内部涡旋等特征。该研究提供了辽宁省冰雹发生临近时刻的环境参量特征及雷达指标阈值,为确定冰雹短临预报预警技术规范奠定了基础。展开更多
基金funded by Program for Changjiang Scholars and Innovative Research Team in University(IRT0978,PCSIRT)the National Natural Science Foundation of China(30270982)
文摘Interleukin-8 homolog (vIL-8) is a chemokine encoded by the genome of Marek's disease virus. Chicken IL-8 (cIL8) is an important chemokine of chickens which plays a role in antiviral activity. To explore the relationship between vIL-8 and cIL8 can help to discern the function of vIL-8. In this study, the amino acid sequences of vIL-8 and cIL8 were aligned. The cIL8 gene was expressed in E. coli and the cIL8 fusion protein was obtained, after induction with IPTG. The protein was separated by SDS-PAGE and the band of interest was excised and minced for mouse immunization. An immunofluorescence test was used to detect vIL-8 expression in insect cells. The results showed that both vIL-8 and cIL8 were typical CXC chemokines in structure, and they had similar key amino acids, known to be important for receptor binding. The result of the immunofluorescence test showed that the mouse anti-cIL8 serum could react with vIL-8 expressed by insect cells. Therefore, vIL-8 shares common antigenic determinants with cIL8, and they may have a common receptor. This feature of vIL-8 suggests that it may participate in the immune evasion of the virus.
文摘研究旨在通过对辽宁省冰雹高发地区的一次典型强雹暴过程进行中尺度特征分析,寻找致雹物理参量特征及雷达临近预警指标,以期为冰雹短临预报预警提供参考。利用常规气象观测资料、葵花卫星、多普勒天气雷达、NCEP/FNL等资料,对发生在辽宁建平地区一次强冰雹天气的天气形势及中尺度特征进行了详细分析。结果表明:此次冰雹过程发生在较强的风切变环境场中,850 h Pa与500 h Pa风向切变>90°,850 h Pa与500 h Pa之间的温度差>26℃,大气层结不稳定;冰雹发生在强对流云团发展至成熟阶段初期,在可见光云图上可以看出明显的纹理和暗影,TBB较长时间维持在-50℃以下,冰雹发生后,TBB梯度值迅速减小;强降雹超级单体呈现弓状,除了具有入流边界、回波悬垂等雷达特征外,还具有高悬的反射率因子核心,即>55 d BZ高度伸展到-20℃以上,以及VIL>60 kg/m^2,VIL密度>4 g/m^3,深厚持久的中气旋维持超级单体内部涡旋等特征。该研究提供了辽宁省冰雹发生临近时刻的环境参量特征及雷达指标阈值,为确定冰雹短临预报预警技术规范奠定了基础。
