Hepatic fibrosis is regulated by the synergistic actions of various cells and cytokines,with the activation and proliferation of hepatic stellate cells(HSCs) being considered the central event in this process.To achie...Hepatic fibrosis is regulated by the synergistic actions of various cells and cytokines,with the activation and proliferation of hepatic stellate cells(HSCs) being considered the central event in this process.To achieve specific targeting of activated hepatic stellate cells(a HSCs) and precise treatment of hepatic fibrosis,this study developed a dual-functional drug delivery system(SIL/c RGD-PEG-PPS PMs) with both targeting and responsive release capabilities.It aims to target the αvβ 3 receptor specifically expressed on the surface of a HSCs using the cyclic peptide c(RGDyk),and to exploit the high reactive oxygen species(ROS) level in the cellular microenvironment to achieve concentrated burst release of drugs at the pathological sites of hepatic fibrosis.Based on multiple assessments,SIL/c RGD-PEG-PPS PMs specifically enhanced the targeted delivery of silybin(SIL) to a HSCs,inhibited the proliferation and migration of a HSCs,and exhibited good biosafety.Additionally,it demonstrated excellent anti-fibrotic activity in fibrotic mice.In summary,this study shows great potential in targeted treatment of hepatic fibrosis and provides a multifunctional tool for advancing the research and therapeutic strategies of hepatic fibrosis.展开更多
While degenerative diseases of the central nervous system are commonly linked to age-related macular degeneration and glaucoma,they have also been infrequently associated with retinitis pigmentosa,a condition defined ...While degenerative diseases of the central nervous system are commonly linked to age-related macular degeneration and glaucoma,they have also been infrequently associated with retinitis pigmentosa,a condition defined by retinal degeneration that can be caused by an isoform of receptor expression enhancing protein 6(REEP6)expressed in rod photoreceptors.In this study,we used REEP6 knockout mice(REEP6^(-/-))and wild-type mice(REEP6^(+/+))to examine neurodegenerative pathology within the visual pathways and neural activity in the primary visual cortex(V1)at three specific time points(1,6,and 10 months)during retinitis pigmentosa progression.Microglial activation was observed in both the retina and the primary visual cortex starting at 1 month of age,but no such activation was detected in the lateral geniculate nucleus at any time point.Not only was increased microglial activation observed at 6 and 10 months within the primary visual cortex of REEP6^(-/-)mice,but also coinciding with elevated levels of phosphorylated Tau expression.At 6 and 10 months of age,primary visual cortex neurons in REEP6^(-/-)mice exhibited reduced responses to grating stimuli and increased spontaneous activity compared with neurons in the primary visual cortex of mice in the control group.Our findings show that retinitis pigmentosa induces neurodegenerative pathology within the visual pathway of mice,particularly in the primary visual cortex,suggesting that ocular disease contributes substantially to central nervous system degeneration.It may provide new clues for the selection of treatment opportunities and the development of therapeutic measures for the subsequent treatment of retinitis pigmentosa or even other retinal degenerative diseases.展开更多
针对含光伏(photovoltaic,PV)、电动汽车(electric vehicle,EV)及家庭电器负荷的智能社区,以车入户(vehicle to home,V2H)的形式将EV纳入家庭需求响应框架,利用EV的双向输能特性并考虑EV充/放电带来的电池容量退化成本,协同PV、电网的...针对含光伏(photovoltaic,PV)、电动汽车(electric vehicle,EV)及家庭电器负荷的智能社区,以车入户(vehicle to home,V2H)的形式将EV纳入家庭需求响应框架,利用EV的双向输能特性并考虑EV充/放电带来的电池容量退化成本,协同PV、电网的实时电价和用户需求的可容忍时延,基于Lyapunov优化理论提出随机环境下V2H用户的EV充/放电调度策略和每户家庭的负荷响应策略,最小化家庭用户的长期平均购电成本。并提出一种智能社区在线能量交易方案,旨在最小化智能社区总的购电成本、最大限度提高社区能源利用率。理论分析和仿真结果表明,所提算法无需实时电价、PV出力、用户负荷需求的先验概率信息,仅基于当前系统状态就可使优化目标趋于最优值,实现家庭用户的能量调度和家庭用户之间的能量共享,减少家庭购电成本,提高用户之间能量交易的灵活性。展开更多
Accurate and real-time traffic-sign detection is a cornerstone of Advanced Driver-Assistance Systems(ADAS)and autonomous vehicles.However,existing one-stage detectors miss distant signs,and two-stage pipelines are imp...Accurate and real-time traffic-sign detection is a cornerstone of Advanced Driver-Assistance Systems(ADAS)and autonomous vehicles.However,existing one-stage detectors miss distant signs,and two-stage pipelines are impractical for embedded deployment.To address this issue,we present YOLO-SMM,a lightweight two-stage framework.This framework is designed to augment the YOLOv8 baseline with three targeted modules.(1)SlimNeck replaces PAN/FPN with a CSP-OSA/GSConv fusion block,reducing parameters and FLOPs without compromising multi-scale detail.(2)The MCA model introduces row-and column-aware weights to selectively amplify small sign regions in cluttered scenes.(3)MPDIoU augments CIoU loss with a corner-distance term,supplying stable gradients for sub-20-pixel boxes and tightening localization.An evaluation of YOLO-SMMon the German Traffic Sign Recognition Benchmark(GTSRB)revealed that it attained 96.3% mAP50 and 93.1% mAP50-90 at a rate of 90.6 frames per second(FPS).This represents an improvement of+1.0% over previous performance benchmarks.Them APat 64×64 resolution was found to be 50% of the maximum attainable value,with an FPS of+8.3 when compared to YOLOv8.This result indicates superior performance in terms of accuracy and speed compared to YOLOv7,YOLOv5,RetinaNet,EfficientDet,and Faster R-CNN,all of which were operated under equivalent conditions.展开更多
In real-world autonomous driving tests,unexpected events such as pedestrians or wild animals suddenly entering the driving path can occur.Conducting actual test drives under various weather conditions may also lead to...In real-world autonomous driving tests,unexpected events such as pedestrians or wild animals suddenly entering the driving path can occur.Conducting actual test drives under various weather conditions may also lead to dangerous situations.Furthermore,autonomous vehicles may operate abnormally in bad weather due to limitations of their sensors and GPS.Driving simulators,which replicate driving conditions nearly identical to those in the real world,can drastically reduce the time and cost required for market entry validation;consequently,they have become widely used.In this paper,we design a virtual driving test environment capable of collecting and verifying SiLS data under adverse weather conditions using multi-source images.The proposed method generates a virtual testing environment that incorporates various events,including weather,time of day,and moving objects,that cannot be easily verified in real-world autonomous driving tests.By setting up scenario-based virtual environment events,multi-source image analysis and verification using real-world DCUs(Data Concentrator Units)with V2X-Car edge cloud can effectively address risk factors that may arise in real-world situations.We tested and validated the proposed method with scenarios employing V2X communication and multi-source image analysis.展开更多
Background: This study focuses on the fabrication and optimization of Ti6Al4V alloy latticestructures produced by the Selective Laser Melting (SLM) process. Such structures areincreasingly used in biomedical implants ...Background: This study focuses on the fabrication and optimization of Ti6Al4V alloy latticestructures produced by the Selective Laser Melting (SLM) process. Such structures areincreasingly used in biomedical implants due to their potential to match the mechanicalproperties of human bone. Key features influencing their performance include porosity ratio,surface roughness, elastic modulus, and yield strength. Achieving a balance between theseparameters is essential for ensuring both mechanical integrity and biological compatibility.Methods: The Taguchi method integrated with Grey Relational Analysis (GRA) wasemployed to optimize the SLM process parameters—laser power (160-240 J), scanningspeed (1000-1500 mm/min), and hatch spacing (0.06-0.12 mm). The optimization aimed toproduce lattice structures with properties closely resembling human bone. Experimentaltrials were conducted to evaluate the effects of these parameters on porosity, surfaceroughness, elastic modulus, and yield strength, followed by statistical and relational analysisto determine the optimal configuration. Results: The results revealed that higher scanningspeed, wider hatch spacing, and lower laser power increased the porosity ratio compared toCAD models. A strong inverse relationship was observed between porosity and both yieldstrength and elastic modulus. Increasing laser power substantially reduced surfaceroughness. Through Taguchi-GRA optimization, the optimal parameter combination wasdetermined as laser power of 240 J, scanning speed of 1250 mm/min, and hatch spacing of0.06 mm. Under these conditions, the obtained values were: modulus of elasticity (0°) = 20GPa, modulus of elasticity (90°) = 18.874 GPa, yield strength (0°) = 265 MPa, yieldstrength (90°) = 260 MPa, porosity = 48.565%, and surface roughness = 6.223 μm.Conclusion: The optimized SLM parameters successfully produced Ti6Al4V latticestructures with mechanical and morphological characteristics compatible with human bone.The study highlights the critical balance between process parameters and structuralfeatures, providing a systematic approach for tailoring lattice structures for biomedicalapplications through Taguchi and GRA-based optimization.展开更多
Friction stir processing(FSP) has emerged as a transformative solid-state technique for enhancing the mechanical performance and microstructural integrity of metallic materials,particularly in the context of additive ...Friction stir processing(FSP) has emerged as a transformative solid-state technique for enhancing the mechanical performance and microstructural integrity of metallic materials,particularly in the context of additive manufacturing(AM).This study demonstrates the effectiveness of FSP as a post-processing strategy for two distinct AM systems:wire arc additive manufacturing(WAAM) of low-carbon steel and selective laser melting(SLM) of Ti6Al4V alloy.In the case of WAAM fabricated steel,FSP significantly refined the coarse dendritic microstructure into ultrafine equiaxed grains,resulting in a 21 %-24 % increase in hardness and enhanced tensile properties at the overlapping regions.Similarly,for SLM fabricated Ti6Al4V,FSP eliminated the columnar prior-β grains and residual porosity,yielding a homogenous α+β structure with improved strengthductility balance and reduced anisotropy.These improvements were attributed to the dynamic recrystallization,conversion of low-angle to high-angle grain boundaries,and homogenization of phase constituents induced by FSP.Despite challenges such as tool wear and fixturing complexity,the study confirms that FSP can reliably bridge the performance gap in AM components by healing solidification defects,mitigating anisotropy,and tailoring the local microstructure.The findings position FSP as a versatile and scalable post-processing technique,crucial for advancing high-performance,application-ready components in aerospace,biomedical,and structural applications.展开更多
Background:—Synovial sarcoma is a rare soft tissue sarcoma.Treatment of synovial sarcoma includes surgery,radiation,pazopanib,and chemotherapy.Targeted therapies,such as B-Raf proto-oncogene,serine/threonine kinase(B...Background:—Synovial sarcoma is a rare soft tissue sarcoma.Treatment of synovial sarcoma includes surgery,radiation,pazopanib,and chemotherapy.Targeted therapies,such as B-Raf proto-oncogene,serine/threonine kinase(BRAF)inhibitors,are emerging as a potential treatment option.We describe the sixth case of a BRAF^(V600E)synovial sarcoma,the first extra-thoracic case.This case is the first to show a complete pathological response to BRAF&mitogen-activated protein kinase kinase(MEK)inhibitors.Case description:—We treated a 22-year-old male with a left groin BRAF^(V600E)synovial sarcoma with doxorubicin,Ifosphamide&Sodium 2-Mercaptoethanesulfonate.When we identified BRAF^(V600E)in the tumor,the BRAF^(V600E)and MEK inhibitors(dabrafenib&trametinib)were initiated,followed by surgery,with a complete pathological response.Nine months after the surgery,a local recurrence prompted the resumption of dabrafenib&trametinib followed by radiotherapy,resulting in complete radiological response and the development of hemophagocytic lymphohistiocytosis treated with corticosteroids with resolution of symptoms.Conclusion:—Panel sequencing of synovial sarcoma can identify targetable mutations.Treatment of BRAF^(V600E)synovial sarcoma with dabrafenib&trametinib can lead to complete pathological response and prolonged radiological response,as well as the rare adverse event of hemophagocytic lymphohistiocytosis.Prospective clinical trials are needed to evaluate the efficacy and safety of BRAF^(V600E)&MEK inhibitors as a therapeutic approach in BRAF^(V600E)synovial sarcoma.展开更多
基金supported by the financial assistance from Natural Science Fund Project of Science and Technology Department of Jilin Province (Nos.YDZJ202301ZYTS141,YDZJ202501ZYTS793)。
文摘Hepatic fibrosis is regulated by the synergistic actions of various cells and cytokines,with the activation and proliferation of hepatic stellate cells(HSCs) being considered the central event in this process.To achieve specific targeting of activated hepatic stellate cells(a HSCs) and precise treatment of hepatic fibrosis,this study developed a dual-functional drug delivery system(SIL/c RGD-PEG-PPS PMs) with both targeting and responsive release capabilities.It aims to target the αvβ 3 receptor specifically expressed on the surface of a HSCs using the cyclic peptide c(RGDyk),and to exploit the high reactive oxygen species(ROS) level in the cellular microenvironment to achieve concentrated burst release of drugs at the pathological sites of hepatic fibrosis.Based on multiple assessments,SIL/c RGD-PEG-PPS PMs specifically enhanced the targeted delivery of silybin(SIL) to a HSCs,inhibited the proliferation and migration of a HSCs,and exhibited good biosafety.Additionally,it demonstrated excellent anti-fibrotic activity in fibrotic mice.In summary,this study shows great potential in targeted treatment of hepatic fibrosis and provides a multifunctional tool for advancing the research and therapeutic strategies of hepatic fibrosis.
基金supported by STI 2030-Major Projects,No.2022ZD0208503(to DY)the Fund of Chinese Academy of Science(“Xi Bu Zhi Guang”Project)(to YY)the Sichuan Science and Technology Program,No.2023YFS0312(to YY).
文摘While degenerative diseases of the central nervous system are commonly linked to age-related macular degeneration and glaucoma,they have also been infrequently associated with retinitis pigmentosa,a condition defined by retinal degeneration that can be caused by an isoform of receptor expression enhancing protein 6(REEP6)expressed in rod photoreceptors.In this study,we used REEP6 knockout mice(REEP6^(-/-))and wild-type mice(REEP6^(+/+))to examine neurodegenerative pathology within the visual pathways and neural activity in the primary visual cortex(V1)at three specific time points(1,6,and 10 months)during retinitis pigmentosa progression.Microglial activation was observed in both the retina and the primary visual cortex starting at 1 month of age,but no such activation was detected in the lateral geniculate nucleus at any time point.Not only was increased microglial activation observed at 6 and 10 months within the primary visual cortex of REEP6^(-/-)mice,but also coinciding with elevated levels of phosphorylated Tau expression.At 6 and 10 months of age,primary visual cortex neurons in REEP6^(-/-)mice exhibited reduced responses to grating stimuli and increased spontaneous activity compared with neurons in the primary visual cortex of mice in the control group.Our findings show that retinitis pigmentosa induces neurodegenerative pathology within the visual pathway of mice,particularly in the primary visual cortex,suggesting that ocular disease contributes substantially to central nervous system degeneration.It may provide new clues for the selection of treatment opportunities and the development of therapeutic measures for the subsequent treatment of retinitis pigmentosa or even other retinal degenerative diseases.
文摘针对含光伏(photovoltaic,PV)、电动汽车(electric vehicle,EV)及家庭电器负荷的智能社区,以车入户(vehicle to home,V2H)的形式将EV纳入家庭需求响应框架,利用EV的双向输能特性并考虑EV充/放电带来的电池容量退化成本,协同PV、电网的实时电价和用户需求的可容忍时延,基于Lyapunov优化理论提出随机环境下V2H用户的EV充/放电调度策略和每户家庭的负荷响应策略,最小化家庭用户的长期平均购电成本。并提出一种智能社区在线能量交易方案,旨在最小化智能社区总的购电成本、最大限度提高社区能源利用率。理论分析和仿真结果表明,所提算法无需实时电价、PV出力、用户负荷需求的先验概率信息,仅基于当前系统状态就可使优化目标趋于最优值,实现家庭用户的能量调度和家庭用户之间的能量共享,减少家庭购电成本,提高用户之间能量交易的灵活性。
基金supported by University of Malaya and Ministry of High Education-Malaysia via Fundamental Research Grant Scheme No.FRGS/1/2023/TK10/UM/02/3.
文摘Accurate and real-time traffic-sign detection is a cornerstone of Advanced Driver-Assistance Systems(ADAS)and autonomous vehicles.However,existing one-stage detectors miss distant signs,and two-stage pipelines are impractical for embedded deployment.To address this issue,we present YOLO-SMM,a lightweight two-stage framework.This framework is designed to augment the YOLOv8 baseline with three targeted modules.(1)SlimNeck replaces PAN/FPN with a CSP-OSA/GSConv fusion block,reducing parameters and FLOPs without compromising multi-scale detail.(2)The MCA model introduces row-and column-aware weights to selectively amplify small sign regions in cluttered scenes.(3)MPDIoU augments CIoU loss with a corner-distance term,supplying stable gradients for sub-20-pixel boxes and tightening localization.An evaluation of YOLO-SMMon the German Traffic Sign Recognition Benchmark(GTSRB)revealed that it attained 96.3% mAP50 and 93.1% mAP50-90 at a rate of 90.6 frames per second(FPS).This represents an improvement of+1.0% over previous performance benchmarks.Them APat 64×64 resolution was found to be 50% of the maximum attainable value,with an FPS of+8.3 when compared to YOLOv8.This result indicates superior performance in terms of accuracy and speed compared to YOLOv7,YOLOv5,RetinaNet,EfficientDet,and Faster R-CNN,all of which were operated under equivalent conditions.
基金supported by Institute of Information and Communications Technology Planning and Evaluation(IITP)grant funded by the Korean government(MSIT)(No.2019-0-01842,Artificial Intelligence Graduate School Program(GIST))supported by Korea Planning&Evaluation Institute of Industrial Technology(KEIT)grant funded by the Ministry of Trade,Industry&Energy(MOTIE,Republic of Korea)(RS-2025-25448249+1 种基金Automotive Industry Technology Development(R&D)Program)supported by the Regional Innovation System&Education(RISE)programthrough the(Gwangju RISE Center),funded by the Ministry of Education(MOE)and the Gwangju Metropolitan City,Republic of Korea(2025-RISE-05-001).
文摘In real-world autonomous driving tests,unexpected events such as pedestrians or wild animals suddenly entering the driving path can occur.Conducting actual test drives under various weather conditions may also lead to dangerous situations.Furthermore,autonomous vehicles may operate abnormally in bad weather due to limitations of their sensors and GPS.Driving simulators,which replicate driving conditions nearly identical to those in the real world,can drastically reduce the time and cost required for market entry validation;consequently,they have become widely used.In this paper,we design a virtual driving test environment capable of collecting and verifying SiLS data under adverse weather conditions using multi-source images.The proposed method generates a virtual testing environment that incorporates various events,including weather,time of day,and moving objects,that cannot be easily verified in real-world autonomous driving tests.By setting up scenario-based virtual environment events,multi-source image analysis and verification using real-world DCUs(Data Concentrator Units)with V2X-Car edge cloud can effectively address risk factors that may arise in real-world situations.We tested and validated the proposed method with scenarios employing V2X communication and multi-source image analysis.
文摘Background: This study focuses on the fabrication and optimization of Ti6Al4V alloy latticestructures produced by the Selective Laser Melting (SLM) process. Such structures areincreasingly used in biomedical implants due to their potential to match the mechanicalproperties of human bone. Key features influencing their performance include porosity ratio,surface roughness, elastic modulus, and yield strength. Achieving a balance between theseparameters is essential for ensuring both mechanical integrity and biological compatibility.Methods: The Taguchi method integrated with Grey Relational Analysis (GRA) wasemployed to optimize the SLM process parameters—laser power (160-240 J), scanningspeed (1000-1500 mm/min), and hatch spacing (0.06-0.12 mm). The optimization aimed toproduce lattice structures with properties closely resembling human bone. Experimentaltrials were conducted to evaluate the effects of these parameters on porosity, surfaceroughness, elastic modulus, and yield strength, followed by statistical and relational analysisto determine the optimal configuration. Results: The results revealed that higher scanningspeed, wider hatch spacing, and lower laser power increased the porosity ratio compared toCAD models. A strong inverse relationship was observed between porosity and both yieldstrength and elastic modulus. Increasing laser power substantially reduced surfaceroughness. Through Taguchi-GRA optimization, the optimal parameter combination wasdetermined as laser power of 240 J, scanning speed of 1250 mm/min, and hatch spacing of0.06 mm. Under these conditions, the obtained values were: modulus of elasticity (0°) = 20GPa, modulus of elasticity (90°) = 18.874 GPa, yield strength (0°) = 265 MPa, yieldstrength (90°) = 260 MPa, porosity = 48.565%, and surface roughness = 6.223 μm.Conclusion: The optimized SLM parameters successfully produced Ti6Al4V latticestructures with mechanical and morphological characteristics compatible with human bone.The study highlights the critical balance between process parameters and structuralfeatures, providing a systematic approach for tailoring lattice structures for biomedicalapplications through Taguchi and GRA-based optimization.
基金funded by the National Natural Science Foundation of China(Grant No.52322508)the R&D Program of Beijing Municipal Education Commission(Grant No.KZ20231000519).
文摘Friction stir processing(FSP) has emerged as a transformative solid-state technique for enhancing the mechanical performance and microstructural integrity of metallic materials,particularly in the context of additive manufacturing(AM).This study demonstrates the effectiveness of FSP as a post-processing strategy for two distinct AM systems:wire arc additive manufacturing(WAAM) of low-carbon steel and selective laser melting(SLM) of Ti6Al4V alloy.In the case of WAAM fabricated steel,FSP significantly refined the coarse dendritic microstructure into ultrafine equiaxed grains,resulting in a 21 %-24 % increase in hardness and enhanced tensile properties at the overlapping regions.Similarly,for SLM fabricated Ti6Al4V,FSP eliminated the columnar prior-β grains and residual porosity,yielding a homogenous α+β structure with improved strengthductility balance and reduced anisotropy.These improvements were attributed to the dynamic recrystallization,conversion of low-angle to high-angle grain boundaries,and homogenization of phase constituents induced by FSP.Despite challenges such as tool wear and fixturing complexity,the study confirms that FSP can reliably bridge the performance gap in AM components by healing solidification defects,mitigating anisotropy,and tailoring the local microstructure.The findings position FSP as a versatile and scalable post-processing technique,crucial for advancing high-performance,application-ready components in aerospace,biomedical,and structural applications.
基金Aviad Zick is funded by ISF,grants number 2279/22&3099/22&TRANSCAN3 EpiNanSarc.
文摘Background:—Synovial sarcoma is a rare soft tissue sarcoma.Treatment of synovial sarcoma includes surgery,radiation,pazopanib,and chemotherapy.Targeted therapies,such as B-Raf proto-oncogene,serine/threonine kinase(BRAF)inhibitors,are emerging as a potential treatment option.We describe the sixth case of a BRAF^(V600E)synovial sarcoma,the first extra-thoracic case.This case is the first to show a complete pathological response to BRAF&mitogen-activated protein kinase kinase(MEK)inhibitors.Case description:—We treated a 22-year-old male with a left groin BRAF^(V600E)synovial sarcoma with doxorubicin,Ifosphamide&Sodium 2-Mercaptoethanesulfonate.When we identified BRAF^(V600E)in the tumor,the BRAF^(V600E)and MEK inhibitors(dabrafenib&trametinib)were initiated,followed by surgery,with a complete pathological response.Nine months after the surgery,a local recurrence prompted the resumption of dabrafenib&trametinib followed by radiotherapy,resulting in complete radiological response and the development of hemophagocytic lymphohistiocytosis treated with corticosteroids with resolution of symptoms.Conclusion:—Panel sequencing of synovial sarcoma can identify targetable mutations.Treatment of BRAF^(V600E)synovial sarcoma with dabrafenib&trametinib can lead to complete pathological response and prolonged radiological response,as well as the rare adverse event of hemophagocytic lymphohistiocytosis.Prospective clinical trials are needed to evaluate the efficacy and safety of BRAF^(V600E)&MEK inhibitors as a therapeutic approach in BRAF^(V600E)synovial sarcoma.
