This paper presents a universal platform "uSensing" to support smartphones to communicate with sensor nodes in Wireless Sensor Networks (WSNs).Since phones have different CPU processers and operating systems...This paper presents a universal platform "uSensing" to support smartphones to communicate with sensor nodes in Wireless Sensor Networks (WSNs).Since phones have different CPU processers and operating systems,it is a challenge to merge these heterogeneities and develop such a universal platform.In this paper,we design both hardware and software to support the "universal" feature of uSensing:1) "uSD" card:an IEEE 802.15.4 physical communication card with SD interface;2) "uSinkWare":a WSNs middleware running on smartphones.Integrated with uSD card and uSinkWare,phones become mobile data sinks to access into WSNs and parse messages from sensor nodes.We demonstrate the proposed uSensing platform in a commercial smartphone to connect with our WSNs testbed,and validate that the smartphone has the same WSNs functions as commercial fixed sink.Additionally,we evaluate the performance of uSensing platform through measuring phone's CPU load and power consumption,and analyze the performance of these metrics theoretically.The results suggest that the phone-based mobile sink has enough capability to serve as a mobile sink of WSNs and can work up to twenty hours due to low power consumption.展开更多
Cervical cancer is a prevalent gynecological malignancy,with approximately 90%of cases attributed to human papillomavirus(HPV)infection.Rapid and accurate nucleic acid detection is one of the leading methods to improv...Cervical cancer is a prevalent gynecological malignancy,with approximately 90%of cases attributed to human papillomavirus(HPV)infection.Rapid and accurate nucleic acid detection is one of the leading methods to improving screening coverage for early cervical cancer diagnosis.However,most existing techniques are usually complex and require expensive instrumentation.Clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated systems have great advantages in nucleic acid detection.We herein combined the CRISPR-Cas12a with a universal dual-mode fluorescent nanoparticles(FNPs)platform to construct a highly sensitive signal-offassay for HPV high-risk subtypes detection.The signal readout module uses a single-stranded DNA linker,which forms a sandwich structure with DNA-functionalized magnetic beads and DNA-functionalized FNPs to generate signals.If trans-cleavage activity was activated by the targets,the linker was consumed and therefore could not form the sandwich structure to produce signals.HPV16 and HPV18 as model targets,the limits of detection as low as 5 pmol/L were successfully achieved without amplification.We validated the feasibility of the real-sample detection using HPV16 and HPV18 pseudo viruses.The proposed method can be easily adapted for other virus or bacterial assays by modifying the CRISPR-derived RNA(crRNA),which shows great potential for clinical diagnosis.展开更多
Type of Company： Government institution. New Preparartions and Late-Model Drug Delivery System Technical Plaform of Peking University School of Pharmaceutical Sciences is a national technical plaform for developing n...Type of Company： Government institution. New Preparartions and Late-Model Drug Delivery System Technical Plaform of Peking University School of Pharmaceutical Sciences is a national technical plaform for developing new and important drugs. The person in charge for the platform is a pharmaceutical science professor of Peking University School of Pharmaceutical Sciences. The plaform selects the following as the main research areas： late-model carrier drug delivery system, biotechnological drug delivery system,展开更多
Lipid rafts(LRs)are relatively well-ordered functional microdomains in cell membranes and play an irreplaceable role in physiological processes as a transduction platform for multiple signaling pathways.Due to their s...Lipid rafts(LRs)are relatively well-ordered functional microdomains in cell membranes and play an irreplaceable role in physiological processes as a transduction platform for multiple signaling pathways.Due to their small size and high spatiotemporal dynamics,it is difficult to perform lipid raftlocalized biomolecule imaging on the surface of living cells.Here,we report a DNA nanotechnology-based platform for reversible manipulation and localized analysis of lipid rafts,which consists of two modules:“patching and coding probe pair”and“fishing probe”.The probe pair is generated by modifying two different sets of connectable DNA structures on a lipid raft-specific protein.After recognizing lipid rafts,the two probes in close proximity are linked by a DNA ligase reaction to form a lipid raft identity(LRID)code.The LR-ID strand patches and stabilizes the lipid raft structure.Interestingly,the raft patches formed can be depatched by restriction endonucleases,providing the first reversible manipulation of the lipid raft structure in living cells.We also designed a“fishing probe”with a DNA hairpin structure using an aptamer that can specifically bind to the target.The probe can cascade the reaction to two input signals“LR-ID”and“target protein”to generate an“off-on”fluorescence switch,allowing imaging and dynamic monitoring of target proteins localized in lipid rafts.By encoding arbitrary targets(in the case of glycans)in lipid rafts,we have created a universal lipid raft-localized imaging platform.This work provides an integrated analytical and manipulative platform to reveal lipid rafts and associated signaling pathways at the molecular level.展开更多
基金supported by the National Natural Science Foundation of China under Grant No.60932005China and Europe Government Cooperation Projects of the Ministry of Science and Technology under Grant No.2010DFA11680the Tsinghua Sci-Tech Project under Grant No.2011THZ0
文摘This paper presents a universal platform "uSensing" to support smartphones to communicate with sensor nodes in Wireless Sensor Networks (WSNs).Since phones have different CPU processers and operating systems,it is a challenge to merge these heterogeneities and develop such a universal platform.In this paper,we design both hardware and software to support the "universal" feature of uSensing:1) "uSD" card:an IEEE 802.15.4 physical communication card with SD interface;2) "uSinkWare":a WSNs middleware running on smartphones.Integrated with uSD card and uSinkWare,phones become mobile data sinks to access into WSNs and parse messages from sensor nodes.We demonstrate the proposed uSensing platform in a commercial smartphone to connect with our WSNs testbed,and validate that the smartphone has the same WSNs functions as commercial fixed sink.Additionally,we evaluate the performance of uSensing platform through measuring phone's CPU load and power consumption,and analyze the performance of these metrics theoretically.The results suggest that the phone-based mobile sink has enough capability to serve as a mobile sink of WSNs and can work up to twenty hours due to low power consumption.
基金financially supported by the National Natural Science Foundation of China(22004005)Special Project on Biomedical Innovation(223777118D)。
文摘Cervical cancer is a prevalent gynecological malignancy,with approximately 90%of cases attributed to human papillomavirus(HPV)infection.Rapid and accurate nucleic acid detection is one of the leading methods to improving screening coverage for early cervical cancer diagnosis.However,most existing techniques are usually complex and require expensive instrumentation.Clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated systems have great advantages in nucleic acid detection.We herein combined the CRISPR-Cas12a with a universal dual-mode fluorescent nanoparticles(FNPs)platform to construct a highly sensitive signal-offassay for HPV high-risk subtypes detection.The signal readout module uses a single-stranded DNA linker,which forms a sandwich structure with DNA-functionalized magnetic beads and DNA-functionalized FNPs to generate signals.If trans-cleavage activity was activated by the targets,the linker was consumed and therefore could not form the sandwich structure to produce signals.HPV16 and HPV18 as model targets,the limits of detection as low as 5 pmol/L were successfully achieved without amplification.We validated the feasibility of the real-sample detection using HPV16 and HPV18 pseudo viruses.The proposed method can be easily adapted for other virus or bacterial assays by modifying the CRISPR-derived RNA(crRNA),which shows great potential for clinical diagnosis.
文摘Type of Company： Government institution. New Preparartions and Late-Model Drug Delivery System Technical Plaform of Peking University School of Pharmaceutical Sciences is a national technical plaform for developing new and important drugs. The person in charge for the platform is a pharmaceutical science professor of Peking University School of Pharmaceutical Sciences. The plaform selects the following as the main research areas： late-model carrier drug delivery system, biotechnological drug delivery system,
基金support from the National Natural Science Foundation of China(21974067,22274073)the Fundamental Research Funds for the Central Universities(020514380309,021414380502,2022300324)the State Key Laboratory of Analytical Chemistry for Life Science(5431ZZXM2305,5431ZZXM2204).
文摘Lipid rafts(LRs)are relatively well-ordered functional microdomains in cell membranes and play an irreplaceable role in physiological processes as a transduction platform for multiple signaling pathways.Due to their small size and high spatiotemporal dynamics,it is difficult to perform lipid raftlocalized biomolecule imaging on the surface of living cells.Here,we report a DNA nanotechnology-based platform for reversible manipulation and localized analysis of lipid rafts,which consists of two modules:“patching and coding probe pair”and“fishing probe”.The probe pair is generated by modifying two different sets of connectable DNA structures on a lipid raft-specific protein.After recognizing lipid rafts,the two probes in close proximity are linked by a DNA ligase reaction to form a lipid raft identity(LRID)code.The LR-ID strand patches and stabilizes the lipid raft structure.Interestingly,the raft patches formed can be depatched by restriction endonucleases,providing the first reversible manipulation of the lipid raft structure in living cells.We also designed a“fishing probe”with a DNA hairpin structure using an aptamer that can specifically bind to the target.The probe can cascade the reaction to two input signals“LR-ID”and“target protein”to generate an“off-on”fluorescence switch,allowing imaging and dynamic monitoring of target proteins localized in lipid rafts.By encoding arbitrary targets(in the case of glycans)in lipid rafts,we have created a universal lipid raft-localized imaging platform.This work provides an integrated analytical and manipulative platform to reveal lipid rafts and associated signaling pathways at the molecular level.
