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Division of high resolution sequence stratigraphy units with wavelet transform of logs in Dagang Oilfield
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作者 Ying ZHANG Baozhi PAN +1 位作者 Buzhou HUANG Linfu XUE 《Global Geology》 2007年第1期69-73,共5页
Division of high resolution sequence stratigraphy units based on wavelet transform of logging data is found to be good at identifying subtle cycles of geological process in Kongnan area of Dagang Oilfield. The anal- y... Division of high resolution sequence stratigraphy units based on wavelet transform of logging data is found to be good at identifying subtle cycles of geological process in Kongnan area of Dagang Oilfield. The anal- ysis of multi-scales gyre of formation with 1-D continuous Dmey wavelet transform of log curve (GR) and I-D discrete Daubechies wavelet transform of log curve (Rt) all make the division of sequence interfaces more objec- tive and precise, which avoids the artificial influence with core analysis and the uncertainty with seismic data and core analysis. 展开更多
关键词 high resolution sequence stratigraphy units logging data wavelet transform
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Analysis on the Problems in Start-up and Debugging of Two 600 MW Generating Units in Yangzhou No.2 Thermal Power Plant
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作者 蒯狄正 《Electricity》 2001年第2期11-15,共5页
The problems including excessive flow of attemperating water for boiler, failure of butterfly valve at the outlet of circulating water pump, burnt-out of thyristor for excitation regulator, load variation rate of CCS ... The problems including excessive flow of attemperating water for boiler, failure of butterfly valve at the outlet of circulating water pump, burnt-out of thyristor for excitation regulator, load variation rate of CCS not complying with the contract target, etc. occurred during start-up and debugging of two 600 MW generating units in Yangzhou No.2 Thermal Power Plant. Through analysis on these problems. the remedial measures were put forward, to which can be referred for similar units. 展开更多
关键词 start-up and debugging problems analysis remedial measures 600 MW generating unit
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Architecture of volcanic sequence and its structural control of Yingcheng Formation in Songliao Basin 被引量:5
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作者 程日辉 王腾飞 +1 位作者 沈艳杰 任延广 《Journal of Central South University》 SCIE EI CAS 2014年第5期2026-2040,共15页
Yingcheng Formation is a set of volcanic strata composed of lava rocks,volcanic clastic rocks and sedimentary rocks,filled in some fault depressions in Songliao Basin,early Cretaceous.The study about litho-facies succ... Yingcheng Formation is a set of volcanic strata composed of lava rocks,volcanic clastic rocks and sedimentary rocks,filled in some fault depressions in Songliao Basin,early Cretaceous.The study about litho-facies succession of Yingcheng Formation in the outcropped area of the southeast margin and in Xujiaweizi fault depression and its distribution based on analysis of seismic data,shows that the sequence of volcanic strata is quite different from the clastic sedimentary sequence.To study the architecture of volcanic sequence and its structural control of Yingcheng Formation in Songliao Basin,in this work,dividing of the volcanic sequence and dating of the sequence boundaries were finished firstly,then displacement and displacement rate of faults were calculated.The results show that,sample ages of top of the first member,the seconde member,and the third member are 127 Ma,115 Ma,110.7 Ma,respectively and sample age of the bottom of the third member is 114.7 Ma.The maximum displacement and displacement rate of the fault 1 are 3 km and 300 m/Ma,respectively,and those of the fault 2 are 3 km and 1000 m/Ma.Studies suggest that,the cooling unit of lava rock or pyroclastic rock is a basic genetic stratigraphic unit in volcanic sequence stratigraphy.Cooling units can construct a parasequence reflecting a volcanic eruption stage.A sequence was superimposed by some parasequences,responding to a volcanic active cycle.There are three types of volcanic sequences in Yingcheng Formation: type of explosion,type of effusion and type of mixed explosion-effusion.The surface of the volcanic sequence,an unconformity surface widely spread and traced in seismic profiles,is a base for analysis of volcanic sequence.The development of volcanic sequence was controlled by faulting,and the curves of fault displacement(rate)can reflect this control.The preservation of volcanic sequence was controlled by the type of volcanic structure and the regional subsidence,also different from that of the sedimentary.The type of volcanic structure of Xujiaweizi was a volcanic depression during the forming of Yingcheng Formation,and the breakdowns of volcanoes and structural subsidence were key factors in the volcanic sequences preservation. 展开更多
关键词 volcanic sequence stratigraphy Yingcheng Formation cooling unit structural subsidence
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On the analysis of the high-resolution sequence stratigraphy and coal accumulating law of jurassic in Ordos Basin 被引量:8
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作者 李增学 韩美莲 +3 位作者 李江涛 余继峰 吕大炜 柳汉丰 《Journal of Coal Science & Engineering(China)》 2008年第1期85-91,共7页
The strata of Jurassic was divided into three tectonic sequences and eight se- quences of third rank,according to the developing feature of the tectonic inconformity and the transforming feature of the depositional sy... The strata of Jurassic was divided into three tectonic sequences and eight se- quences of third rank,according to the developing feature of the tectonic inconformity and the transforming feature of the depositional system tracts.Also the identification and the division of the base-level cycle of different period were carried through.Therefore three cycles of super period,eight cycles of long period,twenty-four cycles of middle period and some cycles of short period were identified.From the overall character of the coal-accu- mulation in the Mesozoic,we can see that the Yan'an formation is of the best nature of coal bearing.When the coal bearing systems of Jurassic were depositing,the Ordos area is the coal accumulating basin of terrene of large scale and located in the same tectonic unit.But the local structure of different part and the paleolandform are different in the basin which resulted in the difference of the depositional environment.So the layer number and the distribution of the thickness of the coal beds are different in the different part of the ba- sin.The coal-accumulating action migrated regularly along with the development,evolve- ment and migration of the depositional systems.The layer numbers of the coal beds, which can be mined,are more in the north and west fringe of the basin,whose distributing area is extensive,and they are more steady in the landscape orientation,also the total thickness is great.Therefore the nature of coal bearing and the coal-accumulating action of different part changed obviously in the space in Ordos area. 展开更多
关键词 high resolution sequence stratigraphy base-level cycle coal accumulating law the migration of rich-coal units
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Precise Pentamers with Diverse Monomer Sequences and Their Thermal Properties 被引量:1
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作者 Rui-Zhe Liu Lei Zhang +1 位作者 Kun-Kun Guo Jiang-Tao Xu 《Chinese Journal of Polymer Science》 SCIE EI CAS CSCD 2022年第5期447-455,共9页
Sequenced-defined oligomer has been emerged as one of the hot topics in polymer chemistry due to its capability of precisely controlling both chain length and monomer sequence.Recent efforts have focused on developmen... Sequenced-defined oligomer has been emerged as one of the hot topics in polymer chemistry due to its capability of precisely controlling both chain length and monomer sequence.Recent efforts have focused on development of synthetic methodologies using state-ofthe-art chemistry tools.However,investigating the impact of minor changes in monomer sequence on physical properties of these materials is still underdeveloped.Herein,four sequenced pentamers are synthesized by a reversible addition-fragmentation chain transfer(RAFT)single unit monomer insertion technique,in which a base pentamer possesses a relatively rigid backbone comprising of five cyclic monomer units.One of the cyclic units in this base pentamer is replaced by an acyclic monomer at different locations(the 1st,3rd and 5th unit)to produce three modified pentamers,which leads to a significant decrease of glass transition temperature(Tg)compared to the base pentamer.Meanwhile,the modified pentamers with identical primary structures but distinct monomer sequences also present different Tg values depending on the position of the acyclic monomer unit.The middle(3rd)position of the acyclic unit causes profound decrease of Tg due to its increased molecular flexibility.These synthetic pentamers have been demonstrated to be excellent oligomeric plasticizers to modulate thermal transitions of bulk polymer materials. 展开更多
关键词 PENTAMER sequence control Single unit monomer insertion Glass transition temperature sequence-defined oligomer
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Survey of Annual and Seasonal Fungal Communities in Japanese <i>Prunus mume</i>Orchard Soil by Next-Generation Sequencing
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作者 Yoshinao Aoki Keiko Fujita +1 位作者 Hiroyuki Shima Shunji Suzuki 《Advances in Microbiology》 2015年第13期817-824,共8页
Fungi play a vital role in the management of soil environment. Although various fungal communities are found in soil, it is difficult to determine the fungal community structure in soil. In this study, we conducted a ... Fungi play a vital role in the management of soil environment. Although various fungal communities are found in soil, it is difficult to determine the fungal community structure in soil. In this study, we conducted a comprehensive survey of fungal communities in Japanese Prunus mume orchard soil from 2010 to 2012 growing seasons using next-generation sequencing technology. Fungal DNA was directly extracted from the soil samples and the internal transcribed spacer 1 region was amplified by PCR and sequenced. We identified 34,826 fungal clone sequences from the soil samples. The fungal clones were sorted into 2132 operational taxonomic units and a majority of the discriminated clone sequences were classified as Ascomycota and Basidiomycota. The number of fungal species belonging to Ascomycota showed increases in June in the three growing seasons. That belonging to Glomeromycota showed increases in August in the three growing seasons. As Ascomycota fungi are wood decomposers and saprotrophs, the results suggested that the number of plant pathogenic fungi increased in Japanese P. mume orchard soil in June. These findings show for the first time the annual and seasonal fungal community structures in Japanese P. mume orchard soil, and are expected to provide valuable clues for improvement when planting new P. mume trees in Japanese orchards. 展开更多
关键词 Fungal Community Structure JAPANESE PRUNUS mume ORCHARD Next-Generation sequencing Operational TAXONOMIC unit Soil Microorganism
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Use of Environmental Sequence Stratigraphy (ESS) as an Environmental Forensic Tool to Identify Chlorinated Solvent Sources at a Complex Site in Silicon Valley, California
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作者 Rick Cramer Jun Lu +2 位作者 Mike Shultz Colin Plank Herb Levine 《Journal of Environmental Protection》 2018年第5期554-566,共13页
This paper presents a case study for a complex contaminated groundwater site impacted by a historical release of chlorinated solvents in Silicon Valley, California. The original conceptual site model (CSM) inferred a ... This paper presents a case study for a complex contaminated groundwater site impacted by a historical release of chlorinated solvents in Silicon Valley, California. The original conceptual site model (CSM) inferred a contaminant migration pathway based on the groundwater gradient interpreted from groundwater elevation data, which is based on the underlying assumption that the subsurface conditions are homogeneous. However, the buried channel deposits render the underlying geology highly heterogeneous, and this heterogeneity plays a significant role in the subsurface migration of contaminants. Chemical fingerprinting evidence suggested that contamination at the downgradient property boundary was related to an off-site contaminant source. But, this alone was not a compelling argument. However, Environmental Sequence Stratigraphy (ESS), a geology-based environmental forensic technique, was applied to define the permeability architecture or the “plumbing” that controls subsurface fluid flow and contaminant migration. First, the geologic and depositional setting was synthesized based on regional geologic data, and representative facies models were identified for the site. Second, the existing CSM and site lithology data were reviewed and existing lithology data were graphically presented to display vertical grain-size patterns. This analysis focused on the nexus between the depositional environment and the site-specific subsurface data resulting in correlations/interpretations between and beyond data points that are based on established stratigraphic principles. The depositional environment results in buried river channels as the primary control on subsurface fluid flow, which defines hydrostratigraphic units (or HSUs). Finally, a hydrostratigraphic CSM that includes maps and cross sections was constructed to depict the HSUs present as a framework to integrate hydro-geology and chemistry data. This study demonstrates that: 1) Highly per-meable buried river channel deposits control subsurface fluid flow and contaminant transport, and have distinct chemical constituents and concentrations (i.e., they represent distinct HSUs), 2) Mapping of such HSUs is feasible with existing boring log data, 3) In settings such as the Santa Clara Valley where groundwater flow is governed by subsurface channel deposits, a hydrostratigraphic mapping approach is superior to a depth-based aquifer zonation approach, and 4) For heterogeneous subsurface, a detailed geology-based definition of the subsurface is an integral component of an environmental forensic analyses to determine contaminant source(s) and pathways. 展开更多
关键词 ENVIRONMENTAL sequence Stratigraphy Hydrostratigraphic units HSU Contaminant SOURCES Source Identification Migration Pathways DEPOSITIONAL Environment Chlorinated Solvents ENVIRONMENTAL Forensic TOOL
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钢桥板单元分布式柔性作业车间成组调度
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作者 朱斌 马骁 +1 位作者 李稷丰 雷景媛 《郑州大学学报(工学版)》 北大核心 2026年第1期41-48,共8页
针对钢桥板单元生产速度过慢会直接制约桥梁工程建设周期的问题,在考虑钢桥板单元的加工工艺路线和生产特点的同时,以最小化最大完工时间为目标,建立了考虑顺序相关作业切换时间和运输时间,面向钢桥板单元加工的分布式柔性作业车间成组... 针对钢桥板单元生产速度过慢会直接制约桥梁工程建设周期的问题,在考虑钢桥板单元的加工工艺路线和生产特点的同时,以最小化最大完工时间为目标,建立了考虑顺序相关作业切换时间和运输时间,面向钢桥板单元加工的分布式柔性作业车间成组调度(DFJGSPST)模型,并提出了基于三层编码的记忆混合遗传禁忌搜索算法(MGATS)。为验证数学模型和智能算法的可行性,以某钢桥板单元生产为例,建立了包括4种板单元组和15台机器的DFJGSPST模型,通过相应的测试算例进行实验验证,并与其他智能算法进行比较分析。实验结果表明:所提的MGATS的相对百分比差异(RPD)的均值为2.74%,低于遗传算法(GA)的3.99%和混合遗传禁忌搜索算法(GATS)的3.13%。MGATS的成功率(SR)为0.15,高于GATS和GA,验证了MGATS在求解DFJGSPST模型中的稳定性和鲁棒性。 展开更多
关键词 钢桥板单元 柔性作业车间 成组调度 顺序相关 运输时间 混合遗传禁忌搜索算法
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基于Transformer和门控循环单元的磷酸化肽从头测序算法
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作者 姚理进 张迪 +2 位作者 周丕宇 曲志坚 王海鹏 《计算机应用》 北大核心 2026年第1期297-304,共8页
利用串联质谱对蛋白质酶切产生的肽段进行测序(称为肽鉴定)是蛋白质组学研究的支撑技术。现有肽段从头测序算法在鉴定具有重要生物学意义的磷酸化修饰肽段时准确度受限,主要原因是磷酸化修饰导致碎裂模式更复杂,易产生中性丢失峰,且磷... 利用串联质谱对蛋白质酶切产生的肽段进行测序(称为肽鉴定)是蛋白质组学研究的支撑技术。现有肽段从头测序算法在鉴定具有重要生物学意义的磷酸化修饰肽段时准确度受限,主要原因是磷酸化修饰导致碎裂模式更复杂,易产生中性丢失峰,且磷酸化肽的质谱图在常规质谱数据中丰度较低。因此,提出基于Transformer和门控循环单元(GRU)的从头测序算法TGNovo。TGNovo引入谱峰连接图,显式建模谱峰间的质量差关系,指导Transformer编码器捕捉谱图特征。Transformer模块与GRU模块分别建模谱图与氨基酸序列的关联以及谱峰间与氨基酸间的依赖关系,二者协同工作以实现肽段重建。相较于完全基于Transformer的从头测序算法Casanovo,TGNovo通过谱峰连接图和GRU模块充分利用谱图先验信息,增强了模型对谱图的建模能力。在跨物种磷酸化肽段评测中,TGNovo在肽水平和氨基酸水平的召回率上比Casanovo分别平均提升了16.5和37.1个百分点;此外,在免疫肽数据集上的实验结果表明,TGNovo鉴定的高可信抗原肽覆盖了数据库搜索结果的86%。 展开更多
关键词 从头测序 TRANSFORMER 门控循环单元 谱峰连接图 磷酸化肽
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考虑生产工序时序耦合的短流程钢铁企业参与电力系统多日尺度下的机组组合优化
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作者 程俊超 包铭磊 +4 位作者 惠恒宇 陆春光 王佳颖 沈然 丁一 《电网技术》 北大核心 2026年第2期641-650,I0077-I0081,共15页
风电和光伏等新能源出力在周时间尺度上的显著变化,会导致电力系统在多日尺度上存在电力电量不平衡问题,这对可调资源提出了更大的需求。工业负荷具有响应容量大、自动化水平高等优点,可为电力系统应对多日电力电量平衡问题提供重要的... 风电和光伏等新能源出力在周时间尺度上的显著变化,会导致电力系统在多日尺度上存在电力电量不平衡问题,这对可调资源提出了更大的需求。工业负荷具有响应容量大、自动化水平高等优点,可为电力系统应对多日电力电量平衡问题提供重要的可调潜力。然而,工业负荷具有离散-连续生产工序混杂、生产多时序耦合、多能耦合等特点,且生产必须满足连续性、安全性等约束,难以直接纳入电力系统调控中。以调节潜力较大的以电炉炼钢为基础的短流程钢铁企业为例,提出考虑生产工序时序耦合的钢铁企业参与电力系统多日机组组合优化方法,为挖掘工业负荷调控潜力并推广至其余负荷提供探索。首先,介绍了短流程钢铁企业的生产流程、用能特性及调节特征,并基于钢铁生产工序的复杂特点建立钢铁生产调节模型;然后考虑电力系统的实际需求,建立钢铁负荷参与电力系统多日机组组合的优化模型,确定钢铁企业的生产计划和机组出力安排。最后基于典型算例,验证了所提方法可在保障钢铁连续性生产的同时为电力系统提供更多调节能力,并促进新能源的消纳。 展开更多
关键词 多日机组组合 生产时序耦合 钢铁企业 离散-连续生产工序 多能耦合
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Whole genome analysis of a Non-O1,Non-O139 Vibrio cholerae isolate from a bacteremia case in Ho Chi Minh City
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作者 Tuan-Loc Le Ngoc-Lan Nguyen +1 位作者 Duc-Khai Luong Hieu Vu-Quang 《Asian Pacific Journal of Tropical Medicine》 2026年第2期94-96,I0062-I0065,共7页
Non-O1/non-O139 Vibrio(V.)cholerae(NOVC)has emerged as a potential pathogen in patients with compromised health conditions[1].We report the whole genome sequencing(WGS)of a rare NOVC sepsis isolate(GenBank Accession:G... Non-O1/non-O139 Vibrio(V.)cholerae(NOVC)has emerged as a potential pathogen in patients with compromised health conditions[1].We report the whole genome sequencing(WGS)of a rare NOVC sepsis isolate(GenBank Accession:GCF_051906115.1)from an 89-year-old male admitted to the Intensive Care Unit(ICU)with septic shock(lactate 6.61 mmol/L)digestive illness. 展开更多
关键词 septic shock intensive care BACTEREMIA intensive care unit whole genome sequencing wgs Whole genome analysis non O non O Vibrio cholerae sepsis
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330 MW火电机组汽轮机阀门流量特性分析与优化
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作者 赵尚羽 杜之正 +1 位作者 邱树良 宋瑞福 《东北电力技术》 2026年第2期44-48,共5页
针对某330 MW火电机组汽轮机开展阀门流量特性分析优化研究,基于锅炉定压运行数据和特定参数,绘制阀门实际流量特性曲线与理想流量特性曲线,通过计算和对比发现二者存在较大非线性度。运用手动修正的方法优化阀门流量函数,使实际流量特... 针对某330 MW火电机组汽轮机开展阀门流量特性分析优化研究,基于锅炉定压运行数据和特定参数,绘制阀门实际流量特性曲线与理想流量特性曲线,通过计算和对比发现二者存在较大非线性度。运用手动修正的方法优化阀门流量函数,使实际流量特性曲线趋于理想状态。试验结果表明,机组修正后的阀门流量函数下的实际流量曲线与理想流量曲线更加吻合,其非线性度小于2%。因此,机组一次调频和AGC的质量得到有效提升,在安全运行的基础上取得了更高的经济效益。 展开更多
关键词 火电机组 汽轮机 阀门流量特性 顺阀控制 等效流量
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Low-power and Low-cost Design of Survival Memory Unit for 1000Base-T Gigabit Ethernet Transceiver 被引量:1
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作者 诸悦 戎蒙恬 《Journal of Shanghai Jiaotong university(Science)》 EI 2006年第4期490-497,共8页
Types of hybrid architectures survivor memory unit (SMU) is presented,which are applicable to IEEE 802.3 ab 1000 Base-T Gigabit Ethernet (GbE) transceiver. Area, power and decoder latency were taken into account and m... Types of hybrid architectures survivor memory unit (SMU) is presented,which are applicable to IEEE 802.3 ab 1000 Base-T Gigabit Ethernet (GbE) transceiver. Area, power and decoder latency were taken into account and most efficient architectures were compared to optimize area/power tradeoff in different kinds of applications. Suitable SMU architectures are given out respectively in area-restrict, power-restrict and latency-restrict designs. A power-efficient architecture was selected in our GbE project. It provides 48% improvement in area and 71% amelioration in power, compared to classical register exchange architecture (REA) SMU. 展开更多
关键词 Gigabit Ethernet decision feedback sequence estimate SURVIVAL MEMORY unit register EXCHANGER ARCHITECTURE trace back ARCHITECTURE
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Bacterial communities associated with natural and commercially grown rooibos (Aspalathus linearis)
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作者 Casparus J.BRINK Anneke POSTMA +3 位作者 Etienne SLABBERT Ferdinand POSTMA A.Muthama MUASYA Karin JACOBS 《Pedosphere》 SCIE CAS CSCD 2020年第6期778-790,共13页
Aspalathus linearis is a commercially important plant species endemic to the Cape Floristic Region of South Africa and is used to produce a herbal tea known as rooibos tea.Symbiotic interactions between A.linearis and... Aspalathus linearis is a commercially important plant species endemic to the Cape Floristic Region of South Africa and is used to produce a herbal tea known as rooibos tea.Symbiotic interactions between A.linearis and soil bacteria play an important role in the survival of Aspalathus plants in the highly nutrient-poor,acidic fynbos soil.The aim of this study was to characterize and compare rhizosphere and bulk soil bacterial communities associated with natural and commercially grown A.linearis,as well as the effect of seasonal changes on these communities.Bacterial communities were characterized using high throughput amplicon sequencing,and their correlations with soil chemical properties were investigated.The N-fixing bacterial community was characterized using terminal restriction fragment length polymorphism and real time quantitative polymerase chain reaction.Actinobacteria,Proteobacteria,and Acidobacteria were the most dominant bacterial phyla detected in this study.Highly similar bacterial communities were associated with natural and commercially grown plants.Significant differences in the bacterial community were observed between rhizosphere and bulk soils collected in the dry season,while no significant differences were detected in the wet season.This study provides insights into bacterial community structure and potential factors shaping bacterial community structure with commercially important A.linearis. 展开更多
关键词 bulk soil FYNBOS N-fixing bacterial community operational taxonomic unit rhizosphere soil 16S rRNA sequencing
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基于微生物DNA测序的高含水井组井间连通性评价
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作者 陆红军 薛纯琦 +5 位作者 常笃 冯飞 苏良银 刘建升 王灼 王硕亮 《钻采工艺》 北大核心 2025年第5期128-135,共8页
鄂尔多斯盆地超低渗油藏储层非均质性强,长期水驱开发导致注采矛盾加剧,井间连通性精准评价成为剩余油挖潜的关键技术瓶颈。针对传统示踪剂监测存在的成本高、时效性差及潜在环境污染等问题,文章开展了基于微生物DNA测序的井间连通性评... 鄂尔多斯盆地超低渗油藏储层非均质性强,长期水驱开发导致注采矛盾加剧,井间连通性精准评价成为剩余油挖潜的关键技术瓶颈。针对传统示踪剂监测存在的成本高、时效性差及潜在环境污染等问题,文章开展了基于微生物DNA测序的井间连通性评价。以长庆油田G271典型高含水井组为研究对象,系统采集注水井(1口)与采油井(4口)的产液样本,通过16S rRNA基因扩增子高通量测序技术,构建以扩增子序列变异(Amplicon Sequence Variants,ASV)为分类单元的微生物群落特征数据库。采用Upset集成图解析注采井间微生物群落重叠特征,结合网络图模型定量表征优势菌群的井间连通关系,并同步开展化学示踪剂对比验证实验。研究结果表明:微生物DNA测序技术可实现注采通道的精准识别,与示踪剂监测结果吻合度高,且操作周期缩短40%以上;油藏原位微生物群落具有显著的“井间指纹”特征,优势菌属(如Pseudomonadales、Burkholderiales)的丰度梯度变化可表征优势渗流通道。该项技术结合了环境友好型和成本效益优势,适应于油藏开发全周期的动态监测,为超低渗油藏高含水期剩余油靶向挖潜提供了创新性技术手段,对实现油田绿色高效开发具有重要应用价值。 展开更多
关键词 高含水井组 井间连通性评价 微生物DNA测序 ASV分类单元 示踪剂监测
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基于宏基因组测序技术的ICU环境耐药菌传播特征
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作者 金丁萍 陆群 +4 位作者 周宏伟 周哲敏 杨晓煊 吴飞宇 胡燕燕 《中华医院感染学杂志》 北大核心 2025年第24期3796-3801,共6页
目的探讨宏基因组二代测序(mNGS)在三个重症监护室(ICU)环境耐药菌监测中的应用价值,揭示耐药菌传播特征,为优化感染控制提供依据。方法选取某三级甲等综合性医院外科重症监护室(SICU)、急诊重症监护室(EICU)和脑科重症监护室(NICU)的... 目的探讨宏基因组二代测序(mNGS)在三个重症监护室(ICU)环境耐药菌监测中的应用价值,揭示耐药菌传播特征,为优化感染控制提供依据。方法选取某三级甲等综合性医院外科重症监护室(SICU)、急诊重症监护室(EICU)和脑科重症监护室(NICU)的物表及空气样本,结合常规细菌培养与mNGS技术,分析微生物群落、耐药基因和毒力因子分布,并通过系统发育树和冗余分析(RDA)探究传播路径。结果培养结果显示,3个ICU物表均检出耐药菌,主要为携带OXA-23型碳青霉烯酶的不动杆菌、携带KPC型碳青霉烯酶的肠杆菌目细菌和假单胞菌。空气样本仅NICU检出耐药菌,主要为利奈唑胺耐药葡萄球菌。mNGS揭示环境中存在复杂细菌群落,其中NICU物表样本多样性最高,并检出与全球流行一致的IC2型鲍曼不动杆菌。RDA显示,不同ICU的物表与空气样本群落差异显著。耐药基因和毒力基因分析显示,SICU物表样本携带多种β-内酰胺酶基因及铁摄取相关毒力基因,NICU样本则富集鲍曼不动杆菌菌毛合成相关毒力基因;空气样本中仅NICU检出携带blaOXA、blaKPC和blaSHV的耐药菌。结论mNGS可精准识别ICU环境耐药菌的分布特征及潜在传播关联,与常规培养形成互补。其应用有助于建立ICU环境耐药菌的主动监测体系,提高院感防控的精准性和时效性。 展开更多
关键词 重症监护室 耐药菌 宏基因组二代测序 细菌培养 院感防控
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基于模块分组的三相MMC简化空间矢量调制方法
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作者 胡存刚 徐婉伦 +1 位作者 杨伟业 刘碧 《电机与控制学报》 北大核心 2025年第3期155-165,共11页
针对模块化多电平变换器(MMC),空间矢量脉宽调制(SVPWM)算法的矢量选择与时序设计较为复杂。为此,提出一种基于子模块分组的MMC简化SVPWM方法。首先,将MMC系统按分组规则划分为若干个虚拟子单元,从而将系统级调制问题分解为单元级控制... 针对模块化多电平变换器(MMC),空间矢量脉宽调制(SVPWM)算法的矢量选择与时序设计较为复杂。为此,提出一种基于子模块分组的MMC简化SVPWM方法。首先,将MMC系统按分组规则划分为若干个虚拟子单元,从而将系统级调制问题分解为单元级控制。其次,针对每个虚拟子单元,根据相参考电压矢量的运行区间,选择合适的电压矢量,并优化作用时间分配,通过载波移相技术协调各子单元间的调制信号相位,实现子模块电容电压、桥臂电压及相间电压的动态均衡。同时,为使系统子模块之间、桥臂之间、各相之间维持稳定,对所选矢量开关状态的作用时序进行设计。最后,对所提算法的可行性与有效性进行验证。仿真与实验结果表明,所提SVPWM在保障MMC良好输出性能的同时,简化了矢量选择和时间计算过程。 展开更多
关键词 模块化多电平变换器 空间矢量调制 子模块分组 矢量时序设计 算法简化 虚拟子单元
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重症监护病房耐碳青霉烯类肺炎克雷伯菌定植与传播研究 被引量:6
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作者 陈翔 高晓东 +5 位作者 周春妹 黄声雷 潘文彦 刘霄 梅静骅 史庆丰 《中国感染控制杂志》 北大核心 2025年第1期77-84,共8页
目的探讨和分析重症监护病房耐碳青霉烯类肺炎克雷伯菌(CRKP)菌株特征与传播途径。方法选取2023年1—10月某院肝外科监护病房CRKP临床感染株17株(临床感染组)、主动筛查株5株(主动筛查组)、环境分离株7株(环境组)进行全基因组测序分析,... 目的探讨和分析重症监护病房耐碳青霉烯类肺炎克雷伯菌(CRKP)菌株特征与传播途径。方法选取2023年1—10月某院肝外科监护病房CRKP临床感染株17株(临床感染组)、主动筛查株5株(主动筛查组)、环境分离株7株(环境组)进行全基因组测序分析,并比较其耐药基因、毒力基因、ST分型差异,根据系统发育树分析其传播途径。结果29株CRKP分别携带4~18种耐药基因,52~98个毒力基因,3组CRKP的耐药基因、毒力基因的数量及基因型别分布比较,差异均无统计学意义(均P>0.05)。ST分型显示,29株CRKP主要包含ST11和ST15两大类,基于核心基因组构建的系统发育树显示,共有7组不同的CRKP高度同源,其中4组有较为明确的流行病学关联。结论重症监护病房的CRKP携带较多的耐药基因、毒力基因,部分菌株ST分型和系统发育树高度同源,存在交叉传播的可能,今后应加强防控措施以减少CRKP的传播。 展开更多
关键词 重症监护病房 耐碳青霉烯类肺炎克雷伯菌 全基因组测序 传播
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南极海域浮游植物群落宏条形码分析中最小分类单元划分方法的比较
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作者 罗培铷 刘璐 +2 位作者 陈作志 张吉昌 王新良 《渔业科学进展》 北大核心 2025年第6期129-142,共14页
近年来,环境DNA宏条形码技术已成为评估海洋浮游植物多样性的重要方法之一,高通量测序结果的分析在一定程度上影响着群落结构信息。本研究对南极海域环境DNA样本进行18S rDNA V4区高通量测序,选取目前应用最广泛的两种最小分类单元划分... 近年来,环境DNA宏条形码技术已成为评估海洋浮游植物多样性的重要方法之一,高通量测序结果的分析在一定程度上影响着群落结构信息。本研究对南极海域环境DNA样本进行18S rDNA V4区高通量测序,选取目前应用最广泛的两种最小分类单元划分方法:97%操作分类单元(OTU)和扩增子序列变体(ASV),对浮游植物群落分析结果进行比较研究。结果显示,两种方法获得的浮游植物群落均主要由甲藻门(Dinoflagellata)、硅藻门(Bacillariophyta)、定鞭藻门(Haptophyta)组成,Simpson和Shannon多样性指数无显著差异,均能较好地反映调查海域浮游植物群落整体特征。两种方法均识别到南极棕囊藻(Phaeocystis antarctica)、无刺环毛藻(Corethron inerme)、双叉角毛藻(Chaetoceros dichaeta)、裸甲藻(Gymnodinium sp.)和原甲藻(Prorocentrum sp.)等南极海域常见优势类群。ASV方法进一步筛选出圆柱拟脆杆藻(Fragilariopsis cylindrus)、克格伦拟脆杆藻(Fragilariopsis kerguelensis)等引起群落差异的重要物种,能够获得更精细的物种信息。相比之下,OTU方法则可能存在注释结果不精准或某些物种相对丰度被低估的现象。因此,当聚焦具体物种时,ASV方法更具应用前景。另外,虽然β多样性在两种方法中没有显著差异,但通过对比研究发现,使用加权的距离指数(Bray-Curtis和加权Unifrac距离)能够增加分析结果的可信度。通过探索最小分类单元划分方法在浮游植物群落结构和多样性分析中表现的差异,可为后续浮游植物群落的多样性研究提供方法选择依据。优化算法和测序区域的选择对浮游植物群落宏条形码多样性研究结果的影响仍需进一步探索。 展开更多
关键词 南极海域 浮游植物群落 18S rDNA 操作分类单元(OTU) 扩增子序列变体(ASV)
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38例重症监护室分离耐碳青霉烯肺炎克雷伯菌分子流行病学特征分析
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作者 孟祥兆 谢新雅 +4 位作者 陈勇 马莉 徐婷 李敏 于洪远 《标记免疫分析与临床》 2025年第9期1975-1980,共6页
随着医疗水平的不断进步以及不同科室医疗功能的不断细化,医院内众多科室开始单独设置重症监护病房,收治危重症患者,由于此类患者抵抗力低,并且会进行多种创伤性治疗,导致患者极易引发感染。碳青霉烯耐药肺炎克雷伯菌(carbapenem-resist... 随着医疗水平的不断进步以及不同科室医疗功能的不断细化,医院内众多科室开始单独设置重症监护病房,收治危重症患者,由于此类患者抵抗力低,并且会进行多种创伤性治疗,导致患者极易引发感染。碳青霉烯耐药肺炎克雷伯菌(carbapenem-resistant Klebsiella pneumoniae,CRKP)作为目前临床报道的ICU检出细菌中最为常见的细菌之一,对其分子流行病学特征的监测对于控制患者的感染有着重要意义。本研究使用全基因组测序及多为点序列分型技术对本院分离的38例CRKP进行回顾性分析,列举其携带的耐药基因及分子分型,希望能为临床与检验工作提供参考帮助。 展开更多
关键词 重症监护病房 碳青霉烯耐药肺炎克雷伯菌 全基因组测序 多位点序列分型 耐药基因
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