It has been reported that endosperm undergoes programmed cell death (PCD) during maize kernel development.Both bz1 (bronze ) and bz2 are anthocyanin biosynthetic genes,and related to development of aleuronic la...It has been reported that endosperm undergoes programmed cell death (PCD) during maize kernel development.Both bz1 (bronze ) and bz2 are anthocyanin biosynthetic genes,and related to development of aleuronic layer of maize seeds.Tyramide signal amplification fluorescence in situ hybridization (TSA FISH) is a novel and high sensitive FISH technique,which is suitable for routine application in plant cytogenetic research.Using this technique,we physically mapped the bz1 gene onto the short arm of chromosome 9 and the long arm of chromosome 1;the percentage distances from centromere to hybridization site were 40.2,75.4 respectively,and the bz2 onto the long arm of chromosome 1 and the short arm of chromosome 5;the percentage distances from centromere to hybridization site were 21.6,15.3 separately.The TSA FISH techniques of small low copy DNA sequences for plants are discussed.展开更多
Fungal infections are hazardous to human health that has drawn wide attention.In this work,a specific and sensitive method combing the recognition of aptamer to(1,3)-β-D-glucan and tyramide signal amplification techn...Fungal infections are hazardous to human health that has drawn wide attention.In this work,a specific and sensitive method combing the recognition of aptamer to(1,3)-β-D-glucan and tyramide signal amplification technology was proposed for the in situ fluorescence imaging of fungi.Fungi could be distinctly observed by fluorescence microscope rapidly.This method provides morphology and diagnostic information for identifying fungi.The combination of aptamer and tyramide signal amplification technology is a promising tool for the detection of fungi,bacteria and even eukaryotic cell with the virtue of biomarkers.展开更多
Exosomal micro RNA(mi RNA) is an ideal candidate of noninvasive biomarker for the early diagnosis of cancer. Sensitive and accurate sensing of abnormal exosomal mi RNA plays essential role for clinical promotion due t...Exosomal micro RNA(mi RNA) is an ideal candidate of noninvasive biomarker for the early diagnosis of cancer. Sensitive and accurate sensing of abnormal exosomal mi RNA plays essential role for clinical promotion due to its close correlation with tumor proliferation and progression. Herein, a microfluidic surface-enhanced Raman scattering(SERS) sensor was proposed for an on-line detection of exosomal mi RNA based on rolling circle amplification(RCA) and tyramine signal amplification(TSA) strategy. The microfluidic chip consists of a magnetic enrichment chamber, a serpentine fluidic mixer and a plasmonic SERS substrate functionalized with capture probes. The released mi RNA activates the capture probe, triggers RCA reaction, and generates a large number of single-stranded DNA products to drive the catalysis of nanotags deposition via TSA, producing numerous “hot spots” to enhance the SERS signals. In merit of the microfluidics chip and nucleic acid-tyramine cascade amplification, the developed SERS sensor significantly improves the sensitivity for the exosomal mi RNA assay, resulting in a limit of detection(LOD) as low as 1 pmol/L and can be successfully applied in the analysis of exosomes secreted from breast tumor cells, which demonstrates the potential utility in practical applications.展开更多
Background： Previous studies demonstrate that eccrine sweat glands are innervated by both cholinergic and adrenergic nerves. However, it is still unknown whether the secretory coils and ducts ofeccrine sweat glands a...Background： Previous studies demonstrate that eccrine sweat glands are innervated by both cholinergic and adrenergic nerves. However, it is still unknown whether the secretory coils and ducts ofeccrine sweat glands are equally innervated by the sympathetic nerve fibers. To welt understand the mechanisms on sweat secretion and reabsorption, the differential innervation of secretory coils and ducts in human eccrine sweat glands was investigated in the study. Methods： From June 2016 to June 2017, six human skins were fixed, paraffin-embedded, and cut into 5 μm-thick sections, followed by costaining for nerve fiber markers protein gene product 9.5 （PGP 9.5）, tyrosine hydroxylase （TH） and vasoactive intestinal peptide （VIP）, and eccrine sweat gland markers K7, S100P, and K14 by combining standard immunofluorescence with tyramide signal amplification （IF-TSA）. Stained sections were observed under the microscope, photographed, and analyzed. Results： The fluorescent signals of PGP 9.5, TH, and VIP were easily visualized, by 1F-TSA, as circular patterns surrounding eccrine sweat glands, but only PGP 9.5 could be observed by standard IF. The IF-TSA method is more sensitivity than standard IF in detecting antigens expressed at low levels. PGP 9.5, TH, and VIP appeared primarily surrounding the secretory coils and sparsely surrounding the sweat ducts. Conclusion： Sweat secretion is mainly controlled by autonomic nerves whereas sweat reabsorption is less affected by nerve activity.展开更多
文摘It has been reported that endosperm undergoes programmed cell death (PCD) during maize kernel development.Both bz1 (bronze ) and bz2 are anthocyanin biosynthetic genes,and related to development of aleuronic layer of maize seeds.Tyramide signal amplification fluorescence in situ hybridization (TSA FISH) is a novel and high sensitive FISH technique,which is suitable for routine application in plant cytogenetic research.Using this technique,we physically mapped the bz1 gene onto the short arm of chromosome 9 and the long arm of chromosome 1;the percentage distances from centromere to hybridization site were 40.2,75.4 respectively,and the bz2 onto the long arm of chromosome 1 and the short arm of chromosome 5;the percentage distances from centromere to hybridization site were 21.6,15.3 separately.The TSA FISH techniques of small low copy DNA sequences for plants are discussed.
基金supported by the National Natural Science Foundation of China(Nos.22174102,21974101,22174101)the Sino-German rapid response funding call for Covid-19 related research(No.C-0008)。
文摘Fungal infections are hazardous to human health that has drawn wide attention.In this work,a specific and sensitive method combing the recognition of aptamer to(1,3)-β-D-glucan and tyramide signal amplification technology was proposed for the in situ fluorescence imaging of fungi.Fungi could be distinctly observed by fluorescence microscope rapidly.This method provides morphology and diagnostic information for identifying fungi.The combination of aptamer and tyramide signal amplification technology is a promising tool for the detection of fungi,bacteria and even eukaryotic cell with the virtue of biomarkers.
基金supported by the National Natural Science Foundation of China (Nos. 31671013, 22004096, 21874105and 21705124)the China Postdoctoral Science Foundation (Nos.2019M663658 and 2020T130096ZX)+2 种基金the Natural Science Basic Research Program of Shaanxi (Nos. 2020JQ-020, 2020JQ-021 and2018JC-001)the Fundamental Research Funds for the Central Universities (No. xzy012020034)“Young Talent Support Plan” of Xi’an Jiaotong University。
文摘Exosomal micro RNA(mi RNA) is an ideal candidate of noninvasive biomarker for the early diagnosis of cancer. Sensitive and accurate sensing of abnormal exosomal mi RNA plays essential role for clinical promotion due to its close correlation with tumor proliferation and progression. Herein, a microfluidic surface-enhanced Raman scattering(SERS) sensor was proposed for an on-line detection of exosomal mi RNA based on rolling circle amplification(RCA) and tyramine signal amplification(TSA) strategy. The microfluidic chip consists of a magnetic enrichment chamber, a serpentine fluidic mixer and a plasmonic SERS substrate functionalized with capture probes. The released mi RNA activates the capture probe, triggers RCA reaction, and generates a large number of single-stranded DNA products to drive the catalysis of nanotags deposition via TSA, producing numerous “hot spots” to enhance the SERS signals. In merit of the microfluidics chip and nucleic acid-tyramine cascade amplification, the developed SERS sensor significantly improves the sensitivity for the exosomal mi RNA assay, resulting in a limit of detection(LOD) as low as 1 pmol/L and can be successfully applied in the analysis of exosomes secreted from breast tumor cells, which demonstrates the potential utility in practical applications.
基金This study was supported by grants from National Natural Science Foundation of China （No. 81772102, and No. 81471882）.
文摘Background： Previous studies demonstrate that eccrine sweat glands are innervated by both cholinergic and adrenergic nerves. However, it is still unknown whether the secretory coils and ducts ofeccrine sweat glands are equally innervated by the sympathetic nerve fibers. To welt understand the mechanisms on sweat secretion and reabsorption, the differential innervation of secretory coils and ducts in human eccrine sweat glands was investigated in the study. Methods： From June 2016 to June 2017, six human skins were fixed, paraffin-embedded, and cut into 5 μm-thick sections, followed by costaining for nerve fiber markers protein gene product 9.5 （PGP 9.5）, tyrosine hydroxylase （TH） and vasoactive intestinal peptide （VIP）, and eccrine sweat gland markers K7, S100P, and K14 by combining standard immunofluorescence with tyramide signal amplification （IF-TSA）. Stained sections were observed under the microscope, photographed, and analyzed. Results： The fluorescent signals of PGP 9.5, TH, and VIP were easily visualized, by 1F-TSA, as circular patterns surrounding eccrine sweat glands, but only PGP 9.5 could be observed by standard IF. The IF-TSA method is more sensitivity than standard IF in detecting antigens expressed at low levels. PGP 9.5, TH, and VIP appeared primarily surrounding the secretory coils and sparsely surrounding the sweat ducts. Conclusion： Sweat secretion is mainly controlled by autonomic nerves whereas sweat reabsorption is less affected by nerve activity.
