We report a case of hemophagocytic syndrome(HPS)secondary to brucellosis,in which typhoidal cells were found in bone marrow,suggesting typhoidal cells present not only in Salmonella typhi infections but also in other ...We report a case of hemophagocytic syndrome(HPS)secondary to brucellosis,in which typhoidal cells were found in bone marrow,suggesting typhoidal cells present not only in Salmonella typhi infections but also in other bacterial infections.Typhoidal cells in bone marrow can be used to quickly identify the presence of bacterial infection pending the results of bone marrow and/or blood cultures.展开更多
Typhoidal (Salmonella enterica serover Typhi and Para-typhi A and B) (TS) and non-typhiodal Salmonella (NTS) gastroenteritis are less reported in Bangladesh. There is also a lack of report on socio-demographic and cli...Typhoidal (Salmonella enterica serover Typhi and Para-typhi A and B) (TS) and non-typhiodal Salmonella (NTS) gastroenteritis are less reported in Bangladesh. There is also a lack of report on socio-demographic and clinical characteristics of Bangladeshi people with typhoid fever and those with non-typhoidal Salmonella (NTS) gastroenteritis. Thus, the present study aimed to compare the socio-demographic, host and clinical characteristics, and seasonal variations between TS and NTS infections among patients attending at a large diarrheal disease hospital in urban Bangladesh. Information were extracted from the database of icddr,b in two different age stratums (0-14 years, and 15 years and above) as 54 with TS and 199 with NTS;and 65 with TS and 239 with NTS respectively after excluding all other pathogens. Randomly selected individuals with diarrhea but without any pathogen in stool constituted the control group (n = 253 and n = 304 respectively). Among 0-14 years, fever [aOR-4.35 (95% CI-1.45-13.06)] and drink unboiled water [aOR-0.22 (95% CI-0.06-0.83)] significantly differed between TS and NTS. Significant associations were observed with lower socio-economic context [aOR-10.02 (95% CI-3.79-26.45)], unbolied drinking water [aOR-2.16 (95% CI-1.05-4.43)], fever [aOR-4.54 (95% CI-1.71-12.03)], pneumonia [aOR-21.57 (95% CI-1.90-245.01)], wasting [aOR-2.60 (95% CI-1.21-5.57)], presence of RBC [aOR-0.09 (95% CI-0.02-0.33], leucocytes [aOR-3.97 (95% CI-1.76-8.99)] and macrophage [aOR-10.71 (95% CI-2.80-41.06)] in stool and alkaline pH [aOR-2.07 (95% CI-1.08-3.97)] when compared with control group. Among ≥15 years, TS was more frequently isolated from individuals with poor socio-economic background [aOR-2.09 (95% CI-1.0-4.33)] and use non-tap drinking water [aOR-0.29 (95% CI-0.13-0.66] compared to their NTS counterparts. Reported lack of formal schooling [aOR-0.65 (95% CI-0.44-0.96)], fever [aOR-2.10 (95% CI-1.03-4.31)], hospital stay (>24 hours) [aOR-1.66 (95% CI-1.05-2.62)], use of intravenous saline [aOR-0.50 (95% CI-0.34-0.76)] and RBC [aOR-2.34 (95% CI-1.23-4.45)] were associated with TS and NTS compared to control group. Socio-demographic, host and clinical characteristics between TS and NTS gastroenteritis were identical;however, findings significantly differed when compared with individuals presented with diarrhea but without any common enteric pathogen in stool.展开更多
BACKGROUND ChatGPT was developed in November 2022 with studies showing its impressive performance in academic examinations,serving as a promising tool to answer questions even on controversial topics.Artificial intell...BACKGROUND ChatGPT was developed in November 2022 with studies showing its impressive performance in academic examinations,serving as a promising tool to answer questions even on controversial topics.Artificial intelligence(AI)achieving surface-level performance does not necessarily equate to a deep understanding of human cognition.The development of artificial wisdom,therefore,necessitates a shift from simply mimicking intelligent behavior to modeling the underlying mechanisms of human wisdom,including emotional understanding,ethical considerations,and contextual awareness.Several theories exist behind the death of Alexander the Great,but no definitive conclusion has been made.AIM To evaluate whether a hybrid approach,combining generative AI(ChatGPT)with human clinical judgment,can meaningfully reassess the cause of death of Alexander the Great.METHODS This is a cross-sectional study using ChatGPT(version 4 Pro).A search was performed with search terms describing the symptoms experienced by Alexander the Great and possible causes of his death:West Nile virus(WNV)encephalitis,poisoning,acute pancreatitis due to excessive alcohol consumption,typhoid fever,and malaria.The historical data and symptomatology were analyzed,weighing evidence and context in a manner akin to human wisdom.RESULTS The most likely cause of death of Alexander the Great,as generated by ChatGPT,was typhoid fever complicated by Guillain-Barrésyndrome(GBS).The hypothesis was based on the alignment between Alexander’s reported symptoms,such as prolonged high fever,severe abdominal pain,neurological decline,and the known clinical presentation of typhoid fever.However,after carefully reviewing the sources mentioned by ChatGPT,many did not back up the idea that typhoid caused GBS and instead pointed to Campylobacter jejuni as the more likely trigger.Other possible causes of death suggested by ChatGPT including acute pancreatitis from excessive alcohol consumption,infectious causes(WNV encephalitis,malaria),and poisoning were less likely.CONCLUSION While ChatGPT initially concluded typhoid fever with GBS as the most plausible cause of death,expert reappraisal of the sources and pathophysiology suggested that C.jejuni-associated GBS was more likely.This study exemplifies how incorporating AI’s pattern recognition with human scrutiny can yield responsible interpretations of historical records.展开更多
Typhoid fever remains a major global health challenge,particularly in regions with limited access to clean water and sanitation.The emergence of multidrug-resistant and extensively drug-resistant strains of Salmonella...Typhoid fever remains a major global health challenge,particularly in regions with limited access to clean water and sanitation.The emergence of multidrug-resistant and extensively drug-resistant strains of Salmonella enterica serovar Typhi highlights the urgent need for alternative treatment strategies.Medicinal plants represent a promising avenue for addressing this crisis,as their bioactive compounds exhibit potent antibacterial properties.According to the World Health Organization,over 20,000 medicinal plant species have been identified as valuable resources for drug discovery,with more than 1,340 demonstrating antimicrobial activity and over 30,000 antimicrobial compounds isolated.Through an extensive review,key phytochemicals with anti-typhoid properties were identified,along with their mechanisms of action and supporting evidence from in vitro and in vivo studies.This review compiles data on plants with demonstrated anti-typhoid activity,highlights challenges such as standardization,regulatory frameworks,and integration into modern healthcare,and explores the role of omics technologies in advancing the discovery of novel plant-based antimicrobial agents.By discussing potential medicinal plant targets and intervention strategies,this work lays the groundwork for future research into plant-derived anti-infective therapies and contributes to the global effort to combat the growing threat of typhoid fever,particularly in resource-limited settings.展开更多
Pullorum disease and fowl typhoid are caused by the Salmonella serovars Gallinarum biovars Pullorum and Gal-linarum,respectively.The prevalence of these diseases varies across regions and is affected by different risk...Pullorum disease and fowl typhoid are caused by the Salmonella serovars Gallinarum biovars Pullorum and Gal-linarum,respectively.The prevalence of these diseases varies across regions and is affected by different risk fac-tors that remain insufficiently documented.To fill this knowledge gap,we have compiled a global dataset for its prevalence,drawing upon a collection of literature from the last seven decades obtained from bilingual databases.However,a more interactive and dynamic platform is still needed for both academics and policymakers to improve biosecurity measures,limit disease transmission,and prevent future outbreaks at the global and local levels.Here,we developed an advanced visualization platform to depict the prevalence of Salmonella Pullorum and Gallinarum,espe-cially in China,which is categorized by geographical region and temporal span.The platform offers a user-friendly,efficient,and visually engaging tool to explore the prevalence of pullorum disease and fowl typhoid between 1945 and 2021 in different regions.Additionally,this platform allows users to understand the influence of various fac-tors,such as breed,farm mode,economic usage and even the sex of the primary host,chickens,on the prevalence of this disease.We further provided a detailed overview of individual province within China.In particular,by selecting two different provinces on the interface,users can quickly visualize and grasp the disparities in disease prevalence between the chosen regions.This interactive toolkit enables a dynamic exploration of the patterns and factors con-tributing to the prevalence of Salmonella Pullorum and Gallinarum.This interactive platform is freely available open source at http://139.9.85.208/.展开更多
Non-typhoid Salmonella is a common foodborne infection.[1]In the setting of immunosuppression,the classical symptom of diarrhea,that is an immune defense mechanism,may be absent,[2,3]allowing the bacteria to hematogen...Non-typhoid Salmonella is a common foodborne infection.[1]In the setting of immunosuppression,the classical symptom of diarrhea,that is an immune defense mechanism,may be absent,[2,3]allowing the bacteria to hematogenous spread and settle in other organs.[4,5]As a result,in the setting of acute pericarditis in immunosuppressed patients,a bacterial etiology must always be considered,which requires pericardiocentesis to complete drainage and pathogen identification.展开更多
Objective: To investigate the in vivo anti-salmonella activity and the safety of aqueous extract of Euphorbia prostratra(E. prostratra), a plant commonly used in Cameroon by traditional healers.Methods: A Salmonella t...Objective: To investigate the in vivo anti-salmonella activity and the safety of aqueous extract of Euphorbia prostratra(E. prostratra), a plant commonly used in Cameroon by traditional healers.Methods: A Salmonella typhimurium-infected rat model was used for the study. The physiological, biochemical and histopathological markers of possible side effects of this extract were studied using standard methods.Results: The extract had a significant effect on the number of viable Salmonella typhimurium recovered from faeces, and could stop salmonellosis after 8 and 10 days of treatment for male and female rats, respectively, with non-toxic doses. However, the biochemical and histopathological analyses revealed that at relatively high doses(≥ 73.48 mg/kg for female and ≥ 122.71 mg/kg for male) the extract could induce liver damage, as illustrated by a rise of serum transaminases' levels and significant inflammation of the parenchyma and portal vein.Side effects were also observed on the kidneys, as shown by both serum and urinary creatinine,and urinary proteins.Conclusions: The overall results indicate that the aqueous extract of E. prostrata has the potential to provide an effective treatment for salmonellosis, including typhoid fever. However,it is necessary to extrapolate these results in large animals, in further studies.展开更多
In this paper,a reliable stochastic numerical analysis for typhoid fever incorporating with protection against infection has been considered.We have compared the solutions of stochastic and deterministic typhoid fever...In this paper,a reliable stochastic numerical analysis for typhoid fever incorporating with protection against infection has been considered.We have compared the solutions of stochastic and deterministic typhoid fever model.It has been shown that the stochastic typhoid fever model is more realistic as compared to the deterministic typhoid fever model.The effect of threshold number T*hold in stochastic typhoid fever model.The proposed framework of the stochastic non-standard finite difference scheme(SNSFD)preserves all dynamical properties like positivity,bounded-ness and dynamical consistency defined by Mickens,R.E.The stochastic numerical simulation of the model showed that increase in protection leads to low disease prevalence in a population.展开更多
We report a case of fulminant hepatic failure associated with Salmonella paratyphi A infection, in a 29-yearold patient who was admitted to the intensive care unit （ICU） with fever of two days, headache and vomiting...We report a case of fulminant hepatic failure associated with Salmonella paratyphi A infection, in a 29-yearold patient who was admitted to the intensive care unit （ICU） with fever of two days, headache and vomiting followed by behavioural changes and disorientation. On examination, the patient appeared acutely ill, agitated, confused, and deeply jaundiced. Temperature 38.5℃, pulse 92/min, blood pressure 130/89 mmHg. Both samples of blood grew S. paratyphi A, which was sensitive to ceftriaxone and ciprofloxacin. Ceftriaxon was administered with high-dose dexamethasone. Two weeks after treatment with ceftriaxon, the patient was discharged in satisfactory condition.展开更多
Objective: Infectious diseases such as typhoid fever lead to the formation of free radicals which can damage the body. Many medicinal plants have antioxidant molecules that neutralize free radicals. The present work e...Objective: Infectious diseases such as typhoid fever lead to the formation of free radicals which can damage the body. Many medicinal plants have antioxidant molecules that neutralize free radicals. The present work evaluated the antioxidant activity and histopathological effects of the dichloromethane fraction of Dichrocephala integrifolia in Salmonella typhi-infected rats.Methods: The S. typhi-infected rats concurrently received daily doses of D. integrifolia extract at doses of 25, 50 and 100 mg/kg body weight or ciprofloxacin(5 mg/kg body weight) for 15 days. Body temperature was measured daily during infection and treatment periods. At the end of treatment period, the animals were sacrificed and biological responses including hematological parameters, superoxide dismutase and catalase activities, and glutathione, malondialdehyde and nitric oxide concentrations were evaluated.Results: The elevated body temperature induced by infection was significantly decreased in animals treated with 25, 50 or 100 mg/kg of the extract. Platelet levels decreased slightly in infected rats, while treatment with the dichloromethane fraction of D. integrifolia significantly increased platelet levels;this response was greater than that elicited by ciprofloxacin. The doses of 50 and 100 mg/kg of the dichloromethane fraction of D. integrifolia notably decreased monocyte and neutrophil values. Activity of superoxide dismutase and catalase and levels of glutathione in the tissues of treated animals were increased significantly(P < 0.01), while malondialdehyde and nitric oxide levels were significantly decreased(P < 0.01), following treatment with the dichloromethane fraction of D. integrifolia.Conclusion: The results of this study show that the dichloromethane fraction of D. integrifolia has protective effects against a series of pathological conditions initiated by oxidation and tissue damage in the course of a S. typhi infection.展开更多
Splenic abscesses are increasingly being identified,possibly due to widespread use of imaging modalities in clinical practice.The commonest clinical features are high grade fever and exclusively localised left upper q...Splenic abscesses are increasingly being identified,possibly due to widespread use of imaging modalities in clinical practice.The commonest clinical features are high grade fever and exclusively localised left upper quadrant abdominal pain.These symptoms are similar to most infectious diseases prevalent in the tropics,making imaging by ultrasonography or computer tomography a necessity in the diagnosis.There are reports from different geographic areas on splenic abscesses associated with typhoid fever.We reported ruptured splenic abscess presenting with peritonitis as a rare and grave complication of typhoid fever.展开更多
To identify bioactive compound in pigeon pea leaves (Cajanus cajan) that inhibits Salmonella thypi (S. thypi).MethodsThe leaf sample was powdered and macerated with methanol and fractioned by liquid-liquid extrac...To identify bioactive compound in pigeon pea leaves (Cajanus cajan) that inhibits Salmonella thypi (S. thypi).MethodsThe leaf sample was powdered and macerated with methanol and fractioned by liquid-liquid extraction using ethyl acetate. The fraction was chromatographed and the isolates were identified for major component with liquid chromatography-mass spectrometry and the antibacterial activity was tested against S. thypi by Kirby-Bauer method.ResultsSubfraction 1 from the ethyl acetate fraction formed a yellowish solid with m/z 272, identified as naringenin. The naringenin-rich fraction shows fairly well inhibitory toward S. thypi in comparison with chloramphenicol.ConclusionsNaringenin shows antibacterial activity and can be developed to treat typhoid.展开更多
AIM:To represent our clinical experience in the treatment of intestinal perforation arising from typhoid fever.METHODS:The records of 22 surgically-treated patients with typhoid intestinal perforation were evaluated r...AIM:To represent our clinical experience in the treatment of intestinal perforation arising from typhoid fever.METHODS:The records of 22 surgically-treated patients with typhoid intestinal perforation were evaluated retrospectively.RESULTS:There were 18 males and 4 females,mean age 37 years(range,8-64 years).Presenting symptoms were fever,abdominal pain,diarrhea or constipation.Sixteen cases were subjected to segmental resection and end-to-end anastomosis,while 3 cases received 2-layered primary repair following debridement,one case with multiple perforations received 2-layered primary repair and end ileostomy,one case received segmental resection and end-to-end anastomosis followed by an end ileostomy,and one case received segmental resection and end ileostomy with mucous fistula operation.Postoperative morbidity was seen in 5 cases and mortality was found in one case.CONCLUSION:Intestinal perforation resulting from Salmonella typhi is an important health problem in Eastern and Southeastern Turkey.In management of this illness,early and appropriate surgical intervention is vital.展开更多
Typhoid fever is a public health challenge mostly concentrated in impoverished, overcrowded areas of the developing world, with lack of safe drinking and sanitation. The most serious complication is typhoid intestinal...Typhoid fever is a public health challenge mostly concentrated in impoverished, overcrowded areas of the developing world, with lack of safe drinking and sanitation. The most serious complication is typhoid intestinal perforation(TIP), observed in 0.8% to 39%, with a striking rate difference between high-income and low-middle-income countries. Although the mortality rate consequent to TIP in resource-poor countries is improved in the last decades, it is still fluctuating from 5% to 80%, due to surgical-and not surgical-related constraints. Huge economic costs and long timelines are required to provide a short-to middle-term solution to the lack of safe water and sanitation. Inherent limitations of the currently available diagnostic tools may lead to under-evaluation as well as over-evaluation of the disease, with consequent delayed treatment or inappropriate, excessive antibiotic use, hence increasing the likelihood of bacterial resistance. There is a need for immunization programs in populations at greatest risk, especially in sub-Saharan Africa. Uniform surgical strategies and guidelines, on the basis of sound or prospective surgical studies and adapted to the local realities, are still lacking. Major drawbacks of the surgical treatment are the frequent delays to surgery, either for late diagnosis or for difficult transports, and the unavailable appropriate intensive care units in most peripheral facilities. As a consequence, poor patient's conditions at presentation, severe peritoneal contamination and unsuitable postoperative care are the foremost determinant of surgical morbidity and mortality.展开更多
We present this rare occurrence of a 17 yr old boy,a known case of congenital hypoparathyroidism, who presented with fever and jaundice for 8 days and 2 episodes of generalised tonic-clonic seizures.Premorbidly patien...We present this rare occurrence of a 17 yr old boy,a known case of congenital hypoparathyroidism, who presented with fever and jaundice for 8 days and 2 episodes of generalised tonic-clonic seizures.Premorbidly patient was on regular oral calcium supplementations with normal serum calcium levels.Investigations revealed severe hypocalcaemia(3.2 mg/dL),low 25 hydroxyvitamin D levels and hypomagnesacmia.The marked elevation of serum bilirubin was accompanied by derangement of liver enzymes.Microbiological investigations were confirmatory for both hepatitis A and typhoid fever.In spite of the aggressive management with intravenous calcium gluconate infusion,refractory hypocalcaemia persisted with recovery only after gradual decline in the bilirubin levels.We inferred that the cholestatic process produced by both acute viral hepatitis A and typhoid fever precipitated this state of refractory hypocalcaemia in the previously well preserved patient.展开更多
Splenic abscess is an uncommon clinical presentation in surgical practice,associated with high morbidity and mortality.Mortality may be 100%if left untreated.Splenic abscess is also rarely encountered as a complicatio...Splenic abscess is an uncommon clinical presentation in surgical practice,associated with high morbidity and mortality.Mortality may be 100%if left untreated.Splenic abscess is also rarely encountered as a complication of typhoid fever.We present here a case of multiple splenic abscesses with neuropsychiatric complications due to typhoid fever,which was managed successfully with splenectomy and other supportive therapies.Another case of single splenic abscess due to enteric fever was treated successfully with CT-guided aspiration and appropriate antibiotics.Being a rare entity in clinical practice,splenic abscess has been poorly studied.Haemalogenous seeding of the spleen due to typhoid is a common cause of splenic abscess in the tropical countries.In multiple or multiloeulated abscesses aspiration usually does not succeed,which happened in our case.Splenectomy remains the definitive choice of treatment.However,Ultra sonography(USG) or CT-guided aspiration may be tried in selective cases.展开更多
Objective:To isolate the Salmonella enterica serovar Typhi(S.typhi) from asymptomatic typhoid carriers in the local population.To assess the antibiotic sensitivity and resistant pattern of S.typhi isolates against via...Objective:To isolate the Salmonella enterica serovar Typhi(S.typhi) from asymptomatic typhoid carriers in the local population.To assess the antibiotic sensitivity and resistant pattern of S.typhi isolates against viable antibiotics and phylogenetic analysis of S.typhi isolates on the basis of 16S rDNA gene.Methods:S.typhi was isolated and identified based on the cultural characteristics on BSA(Bismuth Sulphite Agar),MacConkey agar,agglutination test with specific antiserum and phylogenetic analysis.5.typhi isolates were tested for sensitivity and resistant pattern with a number of viable antibiotics by disc diffusion method.Result:A total of 15 bile samples were collected from the food handlers to screen the typhoid asymptomatic carriers.Positive result was yielded for 3 out of 15 samples.S.typhi isolates showed resistant to amphicillin(100%), tetracyclin(100%),rifampicin(66.5%),ofloxacin(33.5%),cloxacillin(33.5%) and susceptibility to gentamycin(100%),amikacin(100%),chloramphenicol(100%),streptomycin(100%),kanamycin (100%),cprofloxacin(100%),amoxycillin(66.5%) and ofloxacin(66.5%).Conclusions:This study demonstrates the outbreak of typhoid fever occurs through asymptomatic carrier.In addition,this study also reveals the occurrence of considerable drug resistant among the S.typhi isolates.展开更多
Objective:To discuss the prevalence,clinical and laboratory presentations of relapse typhoid fever.Methods:All relapse cases were reviewed to identify the clinical and laboratory presentation of the relapse typhoid fe...Objective:To discuss the prevalence,clinical and laboratory presentations of relapse typhoid fever.Methods:All relapse cases were reviewed to identify the clinical and laboratory presentation of the relapse typhoid fever.Results:Two hundred and forty six patients were admitted to a teaching tertiary hospital in North-eastern state of Malaysia and fourteen(5.69%) relapse cases were identified.The duration of relapse after the patient was discharged was(25.0±9.9) d.The patients presented with fever,diarrhoea,headache,abdominal pain and constipation. The duration of fever before admission in the initial episode[(8.6±4.2) d]was significantly longer than the relapse episode[(5.0±2.5) d](P=0.019).Four patients have hepatomegaly in initial episode and ten in relapse episode(P=0.852).The defervescence days of initial episodes was (3.2±2.2) d,comparing to relapse episode[(2.0±1.8) d]which was statistically not significant (P=0.124).Conclusion:Assumption of the relapse typhoid fever is milder comparing to original episodes based on observation and is not supported by statistical analysis.展开更多
Enteric fever remains a tropical disease of public health significance in Africa, due to its high endemicity and transmission rates, more in sub-Saharan Africa with 7.2 million cases of typhoid fever annually and inci...Enteric fever remains a tropical disease of public health significance in Africa, due to its high endemicity and transmission rates, more in sub-Saharan Africa with 7.2 million cases of typhoid fever annually and incidence rate of 762 per 100000 person-years when compared with Northern Africa with a reported incidence rate of 557 per 100000 person-years and lower. Recent studies show that almost all regions of sub-Saharan Africa are tending towards high incidence rates, especially Central and Western Africa. Though clinically indistinguishable from paratyphoid fever, typhoid fever causes more morbidity and mortality than paratyphoid fever, with a greater threat to children. Risk factors include consumption of contaminated water, patronizing food vendors and a history of contact with a case or a chronic carrier, amongst others. Environmental factors such as the rainy season, open sewers, contaminated water bodies and areas of low elevation have been implicated. Diagnosis in Africa is challenging due to resource constraints, as many centres still depend on clinical diagnosis and serodiagnosis using Widal test, in an era where more sensitive and specific tests exist. The polymerase chain reaction is one of the most sensitive diagnostic methods, while culture (particularly bone marrow) is considered to be one of the most specific. Quinolones (ciprofloxacin) and third-generation cephalosporins, amongst others, remain potent in the management of enteric fever, with resistance to quinolones gradually on the rise. Poor diagnostics, poor antibiotic stewardship and lack of drug (antibiotic) regulation are contributors to the problem of antibiotic resistance in Africa. Prevention of typhoid fever through;vaccination, especially in children is still under investigation, with steady progress being documented. Overall, long term prevention strategies for typhoid fever should be based on improved sourcesof drinking water, good sanitation and hygiene, food safety and poverty alleviation.展开更多
This study analyzes the transmission of typhoid fever caused by Salmonella typhi using a mathematical model thathighlights the significance of delay in its effectiveness.Time delays can affect the nature of patterns a...This study analyzes the transmission of typhoid fever caused by Salmonella typhi using a mathematical model thathighlights the significance of delay in its effectiveness.Time delays can affect the nature of patterns and slow downthe emergence of patterns in infected population density.The analyzed model is expanded with the equilibriumanalysis,reproduction number,and stability analysis.This study aims to establish and explore the non-standardfinite difference(NSFD)scheme for the typhoid fever virus transmission model with a time delay.In addition,the forward Euler method and Runge-Kutta method of order 4(RK-4)are also applied in the present research.Some significant properties,such as convergence,positivity,boundedness,and consistency,are explored,and theproposed scheme preserves all the mentioned properties.The theoretical validation is conducted on how NSFDoutperforms other methods in emulating key aspects of the continuous model,such as positive solution,stability,and equilibrium about delay.Hence,the above analysis also shows some of the limitations of the conventional finitedifference methods,such as forward Euler and RK-4 in simulating such critical behaviors.This becomes moreapparent when using larger steps.This indicated that NSFD is beneficial in identifying the essential characteristicsof the continuous model with higher accuracy than the traditional approaches.展开更多
文摘We report a case of hemophagocytic syndrome(HPS)secondary to brucellosis,in which typhoidal cells were found in bone marrow,suggesting typhoidal cells present not only in Salmonella typhi infections but also in other bacterial infections.Typhoidal cells in bone marrow can be used to quickly identify the presence of bacterial infection pending the results of bone marrow and/or blood cultures.
文摘Typhoidal (Salmonella enterica serover Typhi and Para-typhi A and B) (TS) and non-typhiodal Salmonella (NTS) gastroenteritis are less reported in Bangladesh. There is also a lack of report on socio-demographic and clinical characteristics of Bangladeshi people with typhoid fever and those with non-typhoidal Salmonella (NTS) gastroenteritis. Thus, the present study aimed to compare the socio-demographic, host and clinical characteristics, and seasonal variations between TS and NTS infections among patients attending at a large diarrheal disease hospital in urban Bangladesh. Information were extracted from the database of icddr,b in two different age stratums (0-14 years, and 15 years and above) as 54 with TS and 199 with NTS;and 65 with TS and 239 with NTS respectively after excluding all other pathogens. Randomly selected individuals with diarrhea but without any pathogen in stool constituted the control group (n = 253 and n = 304 respectively). Among 0-14 years, fever [aOR-4.35 (95% CI-1.45-13.06)] and drink unboiled water [aOR-0.22 (95% CI-0.06-0.83)] significantly differed between TS and NTS. Significant associations were observed with lower socio-economic context [aOR-10.02 (95% CI-3.79-26.45)], unbolied drinking water [aOR-2.16 (95% CI-1.05-4.43)], fever [aOR-4.54 (95% CI-1.71-12.03)], pneumonia [aOR-21.57 (95% CI-1.90-245.01)], wasting [aOR-2.60 (95% CI-1.21-5.57)], presence of RBC [aOR-0.09 (95% CI-0.02-0.33], leucocytes [aOR-3.97 (95% CI-1.76-8.99)] and macrophage [aOR-10.71 (95% CI-2.80-41.06)] in stool and alkaline pH [aOR-2.07 (95% CI-1.08-3.97)] when compared with control group. Among ≥15 years, TS was more frequently isolated from individuals with poor socio-economic background [aOR-2.09 (95% CI-1.0-4.33)] and use non-tap drinking water [aOR-0.29 (95% CI-0.13-0.66] compared to their NTS counterparts. Reported lack of formal schooling [aOR-0.65 (95% CI-0.44-0.96)], fever [aOR-2.10 (95% CI-1.03-4.31)], hospital stay (>24 hours) [aOR-1.66 (95% CI-1.05-2.62)], use of intravenous saline [aOR-0.50 (95% CI-0.34-0.76)] and RBC [aOR-2.34 (95% CI-1.23-4.45)] were associated with TS and NTS compared to control group. Socio-demographic, host and clinical characteristics between TS and NTS gastroenteritis were identical;however, findings significantly differed when compared with individuals presented with diarrhea but without any common enteric pathogen in stool.
文摘BACKGROUND ChatGPT was developed in November 2022 with studies showing its impressive performance in academic examinations,serving as a promising tool to answer questions even on controversial topics.Artificial intelligence(AI)achieving surface-level performance does not necessarily equate to a deep understanding of human cognition.The development of artificial wisdom,therefore,necessitates a shift from simply mimicking intelligent behavior to modeling the underlying mechanisms of human wisdom,including emotional understanding,ethical considerations,and contextual awareness.Several theories exist behind the death of Alexander the Great,but no definitive conclusion has been made.AIM To evaluate whether a hybrid approach,combining generative AI(ChatGPT)with human clinical judgment,can meaningfully reassess the cause of death of Alexander the Great.METHODS This is a cross-sectional study using ChatGPT(version 4 Pro).A search was performed with search terms describing the symptoms experienced by Alexander the Great and possible causes of his death:West Nile virus(WNV)encephalitis,poisoning,acute pancreatitis due to excessive alcohol consumption,typhoid fever,and malaria.The historical data and symptomatology were analyzed,weighing evidence and context in a manner akin to human wisdom.RESULTS The most likely cause of death of Alexander the Great,as generated by ChatGPT,was typhoid fever complicated by Guillain-Barrésyndrome(GBS).The hypothesis was based on the alignment between Alexander’s reported symptoms,such as prolonged high fever,severe abdominal pain,neurological decline,and the known clinical presentation of typhoid fever.However,after carefully reviewing the sources mentioned by ChatGPT,many did not back up the idea that typhoid caused GBS and instead pointed to Campylobacter jejuni as the more likely trigger.Other possible causes of death suggested by ChatGPT including acute pancreatitis from excessive alcohol consumption,infectious causes(WNV encephalitis,malaria),and poisoning were less likely.CONCLUSION While ChatGPT initially concluded typhoid fever with GBS as the most plausible cause of death,expert reappraisal of the sources and pathophysiology suggested that C.jejuni-associated GBS was more likely.This study exemplifies how incorporating AI’s pattern recognition with human scrutiny can yield responsible interpretations of historical records.
文摘Typhoid fever remains a major global health challenge,particularly in regions with limited access to clean water and sanitation.The emergence of multidrug-resistant and extensively drug-resistant strains of Salmonella enterica serovar Typhi highlights the urgent need for alternative treatment strategies.Medicinal plants represent a promising avenue for addressing this crisis,as their bioactive compounds exhibit potent antibacterial properties.According to the World Health Organization,over 20,000 medicinal plant species have been identified as valuable resources for drug discovery,with more than 1,340 demonstrating antimicrobial activity and over 30,000 antimicrobial compounds isolated.Through an extensive review,key phytochemicals with anti-typhoid properties were identified,along with their mechanisms of action and supporting evidence from in vitro and in vivo studies.This review compiles data on plants with demonstrated anti-typhoid activity,highlights challenges such as standardization,regulatory frameworks,and integration into modern healthcare,and explores the role of omics technologies in advancing the discovery of novel plant-based antimicrobial agents.By discussing potential medicinal plant targets and intervention strategies,this work lays the groundwork for future research into plant-derived anti-infective therapies and contributes to the global effort to combat the growing threat of typhoid fever,particularly in resource-limited settings.
基金supported by the National Program on the Key Research Project of China(2022YFC2604201)the Zhejiang Provincial Natural Science Founda-tion of China(LZ24C180002+2 种基金LR19C180001)the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(2021JJLH0083)the Open Project Program of the Jiangsu Key Laboratory of Zoonosis(R1902).
文摘Pullorum disease and fowl typhoid are caused by the Salmonella serovars Gallinarum biovars Pullorum and Gal-linarum,respectively.The prevalence of these diseases varies across regions and is affected by different risk fac-tors that remain insufficiently documented.To fill this knowledge gap,we have compiled a global dataset for its prevalence,drawing upon a collection of literature from the last seven decades obtained from bilingual databases.However,a more interactive and dynamic platform is still needed for both academics and policymakers to improve biosecurity measures,limit disease transmission,and prevent future outbreaks at the global and local levels.Here,we developed an advanced visualization platform to depict the prevalence of Salmonella Pullorum and Gallinarum,espe-cially in China,which is categorized by geographical region and temporal span.The platform offers a user-friendly,efficient,and visually engaging tool to explore the prevalence of pullorum disease and fowl typhoid between 1945 and 2021 in different regions.Additionally,this platform allows users to understand the influence of various fac-tors,such as breed,farm mode,economic usage and even the sex of the primary host,chickens,on the prevalence of this disease.We further provided a detailed overview of individual province within China.In particular,by selecting two different provinces on the interface,users can quickly visualize and grasp the disparities in disease prevalence between the chosen regions.This interactive toolkit enables a dynamic exploration of the patterns and factors con-tributing to the prevalence of Salmonella Pullorum and Gallinarum.This interactive platform is freely available open source at http://139.9.85.208/.
文摘Non-typhoid Salmonella is a common foodborne infection.[1]In the setting of immunosuppression,the classical symptom of diarrhea,that is an immune defense mechanism,may be absent,[2,3]allowing the bacteria to hematogenous spread and settle in other organs.[4,5]As a result,in the setting of acute pericarditis in immunosuppressed patients,a bacterial etiology must always be considered,which requires pericardiocentesis to complete drainage and pathogen identification.
文摘Objective: To investigate the in vivo anti-salmonella activity and the safety of aqueous extract of Euphorbia prostratra(E. prostratra), a plant commonly used in Cameroon by traditional healers.Methods: A Salmonella typhimurium-infected rat model was used for the study. The physiological, biochemical and histopathological markers of possible side effects of this extract were studied using standard methods.Results: The extract had a significant effect on the number of viable Salmonella typhimurium recovered from faeces, and could stop salmonellosis after 8 and 10 days of treatment for male and female rats, respectively, with non-toxic doses. However, the biochemical and histopathological analyses revealed that at relatively high doses(≥ 73.48 mg/kg for female and ≥ 122.71 mg/kg for male) the extract could induce liver damage, as illustrated by a rise of serum transaminases' levels and significant inflammation of the parenchyma and portal vein.Side effects were also observed on the kidneys, as shown by both serum and urinary creatinine,and urinary proteins.Conclusions: The overall results indicate that the aqueous extract of E. prostrata has the potential to provide an effective treatment for salmonellosis, including typhoid fever. However,it is necessary to extrapolate these results in large animals, in further studies.
文摘In this paper,a reliable stochastic numerical analysis for typhoid fever incorporating with protection against infection has been considered.We have compared the solutions of stochastic and deterministic typhoid fever model.It has been shown that the stochastic typhoid fever model is more realistic as compared to the deterministic typhoid fever model.The effect of threshold number T*hold in stochastic typhoid fever model.The proposed framework of the stochastic non-standard finite difference scheme(SNSFD)preserves all dynamical properties like positivity,bounded-ness and dynamical consistency defined by Mickens,R.E.The stochastic numerical simulation of the model showed that increase in protection leads to low disease prevalence in a population.
文摘We report a case of fulminant hepatic failure associated with Salmonella paratyphi A infection, in a 29-yearold patient who was admitted to the intensive care unit （ICU） with fever of two days, headache and vomiting followed by behavioural changes and disorientation. On examination, the patient appeared acutely ill, agitated, confused, and deeply jaundiced. Temperature 38.5℃, pulse 92/min, blood pressure 130/89 mmHg. Both samples of blood grew S. paratyphi A, which was sensitive to ceftriaxone and ciprofloxacin. Ceftriaxon was administered with high-dose dexamethasone. Two weeks after treatment with ceftriaxon, the patient was discharged in satisfactory condition.
文摘Objective: Infectious diseases such as typhoid fever lead to the formation of free radicals which can damage the body. Many medicinal plants have antioxidant molecules that neutralize free radicals. The present work evaluated the antioxidant activity and histopathological effects of the dichloromethane fraction of Dichrocephala integrifolia in Salmonella typhi-infected rats.Methods: The S. typhi-infected rats concurrently received daily doses of D. integrifolia extract at doses of 25, 50 and 100 mg/kg body weight or ciprofloxacin(5 mg/kg body weight) for 15 days. Body temperature was measured daily during infection and treatment periods. At the end of treatment period, the animals were sacrificed and biological responses including hematological parameters, superoxide dismutase and catalase activities, and glutathione, malondialdehyde and nitric oxide concentrations were evaluated.Results: The elevated body temperature induced by infection was significantly decreased in animals treated with 25, 50 or 100 mg/kg of the extract. Platelet levels decreased slightly in infected rats, while treatment with the dichloromethane fraction of D. integrifolia significantly increased platelet levels;this response was greater than that elicited by ciprofloxacin. The doses of 50 and 100 mg/kg of the dichloromethane fraction of D. integrifolia notably decreased monocyte and neutrophil values. Activity of superoxide dismutase and catalase and levels of glutathione in the tissues of treated animals were increased significantly(P < 0.01), while malondialdehyde and nitric oxide levels were significantly decreased(P < 0.01), following treatment with the dichloromethane fraction of D. integrifolia.Conclusion: The results of this study show that the dichloromethane fraction of D. integrifolia has protective effects against a series of pathological conditions initiated by oxidation and tissue damage in the course of a S. typhi infection.
文摘Splenic abscesses are increasingly being identified,possibly due to widespread use of imaging modalities in clinical practice.The commonest clinical features are high grade fever and exclusively localised left upper quadrant abdominal pain.These symptoms are similar to most infectious diseases prevalent in the tropics,making imaging by ultrasonography or computer tomography a necessity in the diagnosis.There are reports from different geographic areas on splenic abscesses associated with typhoid fever.We reported ruptured splenic abscess presenting with peritonitis as a rare and grave complication of typhoid fever.
文摘To identify bioactive compound in pigeon pea leaves (Cajanus cajan) that inhibits Salmonella thypi (S. thypi).MethodsThe leaf sample was powdered and macerated with methanol and fractioned by liquid-liquid extraction using ethyl acetate. The fraction was chromatographed and the isolates were identified for major component with liquid chromatography-mass spectrometry and the antibacterial activity was tested against S. thypi by Kirby-Bauer method.ResultsSubfraction 1 from the ethyl acetate fraction formed a yellowish solid with m/z 272, identified as naringenin. The naringenin-rich fraction shows fairly well inhibitory toward S. thypi in comparison with chloramphenicol.ConclusionsNaringenin shows antibacterial activity and can be developed to treat typhoid.
文摘AIM:To represent our clinical experience in the treatment of intestinal perforation arising from typhoid fever.METHODS:The records of 22 surgically-treated patients with typhoid intestinal perforation were evaluated retrospectively.RESULTS:There were 18 males and 4 females,mean age 37 years(range,8-64 years).Presenting symptoms were fever,abdominal pain,diarrhea or constipation.Sixteen cases were subjected to segmental resection and end-to-end anastomosis,while 3 cases received 2-layered primary repair following debridement,one case with multiple perforations received 2-layered primary repair and end ileostomy,one case received segmental resection and end-to-end anastomosis followed by an end ileostomy,and one case received segmental resection and end ileostomy with mucous fistula operation.Postoperative morbidity was seen in 5 cases and mortality was found in one case.CONCLUSION:Intestinal perforation resulting from Salmonella typhi is an important health problem in Eastern and Southeastern Turkey.In management of this illness,early and appropriate surgical intervention is vital.
文摘Typhoid fever is a public health challenge mostly concentrated in impoverished, overcrowded areas of the developing world, with lack of safe drinking and sanitation. The most serious complication is typhoid intestinal perforation(TIP), observed in 0.8% to 39%, with a striking rate difference between high-income and low-middle-income countries. Although the mortality rate consequent to TIP in resource-poor countries is improved in the last decades, it is still fluctuating from 5% to 80%, due to surgical-and not surgical-related constraints. Huge economic costs and long timelines are required to provide a short-to middle-term solution to the lack of safe water and sanitation. Inherent limitations of the currently available diagnostic tools may lead to under-evaluation as well as over-evaluation of the disease, with consequent delayed treatment or inappropriate, excessive antibiotic use, hence increasing the likelihood of bacterial resistance. There is a need for immunization programs in populations at greatest risk, especially in sub-Saharan Africa. Uniform surgical strategies and guidelines, on the basis of sound or prospective surgical studies and adapted to the local realities, are still lacking. Major drawbacks of the surgical treatment are the frequent delays to surgery, either for late diagnosis or for difficult transports, and the unavailable appropriate intensive care units in most peripheral facilities. As a consequence, poor patient's conditions at presentation, severe peritoneal contamination and unsuitable postoperative care are the foremost determinant of surgical morbidity and mortality.
文摘We present this rare occurrence of a 17 yr old boy,a known case of congenital hypoparathyroidism, who presented with fever and jaundice for 8 days and 2 episodes of generalised tonic-clonic seizures.Premorbidly patient was on regular oral calcium supplementations with normal serum calcium levels.Investigations revealed severe hypocalcaemia(3.2 mg/dL),low 25 hydroxyvitamin D levels and hypomagnesacmia.The marked elevation of serum bilirubin was accompanied by derangement of liver enzymes.Microbiological investigations were confirmatory for both hepatitis A and typhoid fever.In spite of the aggressive management with intravenous calcium gluconate infusion,refractory hypocalcaemia persisted with recovery only after gradual decline in the bilirubin levels.We inferred that the cholestatic process produced by both acute viral hepatitis A and typhoid fever precipitated this state of refractory hypocalcaemia in the previously well preserved patient.
文摘Splenic abscess is an uncommon clinical presentation in surgical practice,associated with high morbidity and mortality.Mortality may be 100%if left untreated.Splenic abscess is also rarely encountered as a complication of typhoid fever.We present here a case of multiple splenic abscesses with neuropsychiatric complications due to typhoid fever,which was managed successfully with splenectomy and other supportive therapies.Another case of single splenic abscess due to enteric fever was treated successfully with CT-guided aspiration and appropriate antibiotics.Being a rare entity in clinical practice,splenic abscess has been poorly studied.Haemalogenous seeding of the spleen due to typhoid is a common cause of splenic abscess in the tropical countries.In multiple or multiloeulated abscesses aspiration usually does not succeed,which happened in our case.Splenectomy remains the definitive choice of treatment.However,Ultra sonography(USG) or CT-guided aspiration may be tried in selective cases.
文摘Objective:To isolate the Salmonella enterica serovar Typhi(S.typhi) from asymptomatic typhoid carriers in the local population.To assess the antibiotic sensitivity and resistant pattern of S.typhi isolates against viable antibiotics and phylogenetic analysis of S.typhi isolates on the basis of 16S rDNA gene.Methods:S.typhi was isolated and identified based on the cultural characteristics on BSA(Bismuth Sulphite Agar),MacConkey agar,agglutination test with specific antiserum and phylogenetic analysis.5.typhi isolates were tested for sensitivity and resistant pattern with a number of viable antibiotics by disc diffusion method.Result:A total of 15 bile samples were collected from the food handlers to screen the typhoid asymptomatic carriers.Positive result was yielded for 3 out of 15 samples.S.typhi isolates showed resistant to amphicillin(100%), tetracyclin(100%),rifampicin(66.5%),ofloxacin(33.5%),cloxacillin(33.5%) and susceptibility to gentamycin(100%),amikacin(100%),chloramphenicol(100%),streptomycin(100%),kanamycin (100%),cprofloxacin(100%),amoxycillin(66.5%) and ofloxacin(66.5%).Conclusions:This study demonstrates the outbreak of typhoid fever occurs through asymptomatic carrier.In addition,this study also reveals the occurrence of considerable drug resistant among the S.typhi isolates.
文摘Objective:To discuss the prevalence,clinical and laboratory presentations of relapse typhoid fever.Methods:All relapse cases were reviewed to identify the clinical and laboratory presentation of the relapse typhoid fever.Results:Two hundred and forty six patients were admitted to a teaching tertiary hospital in North-eastern state of Malaysia and fourteen(5.69%) relapse cases were identified.The duration of relapse after the patient was discharged was(25.0±9.9) d.The patients presented with fever,diarrhoea,headache,abdominal pain and constipation. The duration of fever before admission in the initial episode[(8.6±4.2) d]was significantly longer than the relapse episode[(5.0±2.5) d](P=0.019).Four patients have hepatomegaly in initial episode and ten in relapse episode(P=0.852).The defervescence days of initial episodes was (3.2±2.2) d,comparing to relapse episode[(2.0±1.8) d]which was statistically not significant (P=0.124).Conclusion:Assumption of the relapse typhoid fever is milder comparing to original episodes based on observation and is not supported by statistical analysis.
文摘Enteric fever remains a tropical disease of public health significance in Africa, due to its high endemicity and transmission rates, more in sub-Saharan Africa with 7.2 million cases of typhoid fever annually and incidence rate of 762 per 100000 person-years when compared with Northern Africa with a reported incidence rate of 557 per 100000 person-years and lower. Recent studies show that almost all regions of sub-Saharan Africa are tending towards high incidence rates, especially Central and Western Africa. Though clinically indistinguishable from paratyphoid fever, typhoid fever causes more morbidity and mortality than paratyphoid fever, with a greater threat to children. Risk factors include consumption of contaminated water, patronizing food vendors and a history of contact with a case or a chronic carrier, amongst others. Environmental factors such as the rainy season, open sewers, contaminated water bodies and areas of low elevation have been implicated. Diagnosis in Africa is challenging due to resource constraints, as many centres still depend on clinical diagnosis and serodiagnosis using Widal test, in an era where more sensitive and specific tests exist. The polymerase chain reaction is one of the most sensitive diagnostic methods, while culture (particularly bone marrow) is considered to be one of the most specific. Quinolones (ciprofloxacin) and third-generation cephalosporins, amongst others, remain potent in the management of enteric fever, with resistance to quinolones gradually on the rise. Poor diagnostics, poor antibiotic stewardship and lack of drug (antibiotic) regulation are contributors to the problem of antibiotic resistance in Africa. Prevention of typhoid fever through;vaccination, especially in children is still under investigation, with steady progress being documented. Overall, long term prevention strategies for typhoid fever should be based on improved sourcesof drinking water, good sanitation and hygiene, food safety and poverty alleviation.
基金supported by Prince Sultan University through TAS research lab。
文摘This study analyzes the transmission of typhoid fever caused by Salmonella typhi using a mathematical model thathighlights the significance of delay in its effectiveness.Time delays can affect the nature of patterns and slow downthe emergence of patterns in infected population density.The analyzed model is expanded with the equilibriumanalysis,reproduction number,and stability analysis.This study aims to establish and explore the non-standardfinite difference(NSFD)scheme for the typhoid fever virus transmission model with a time delay.In addition,the forward Euler method and Runge-Kutta method of order 4(RK-4)are also applied in the present research.Some significant properties,such as convergence,positivity,boundedness,and consistency,are explored,and theproposed scheme preserves all the mentioned properties.The theoretical validation is conducted on how NSFDoutperforms other methods in emulating key aspects of the continuous model,such as positive solution,stability,and equilibrium about delay.Hence,the above analysis also shows some of the limitations of the conventional finitedifference methods,such as forward Euler and RK-4 in simulating such critical behaviors.This becomes moreapparent when using larger steps.This indicated that NSFD is beneficial in identifying the essential characteristicsof the continuous model with higher accuracy than the traditional approaches.
