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Estimation of Generalized Pareto under an Adaptive Type-II Progressive Censoring 被引量:2
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作者 Mohamed A. W. Mahmoud Ahmed A. Soliman +1 位作者 Ahmed H. Abd Ellah Rashad M. El-Sagheer 《Intelligent Information Management》 2013年第3期73-83,共11页
In this paper, based on a new type of censoring scheme called an adaptive type-II progressive censoring scheme introduce by Ng et al. [1], Naval Research Logistics is considered. Based on this type of censoring the ma... In this paper, based on a new type of censoring scheme called an adaptive type-II progressive censoring scheme introduce by Ng et al. [1], Naval Research Logistics is considered. Based on this type of censoring the maximum likelihood estimation (MLE), Bayes estimation, and parametric bootstrap method are used for estimating the unknown parameters. Also, we propose to apply Markov chain Monte Carlo (MCMC) technique to carry out a Bayesian estimation procedure and in turn calculate the credible intervals. Point estimation and confidence intervals based on maximum likelihood and bootstrap method are also proposed. The approximate Bayes estimators obtained under the assumptions of non-informative priors, are compared with the maximum likelihood estimators. Numerical examples using real data set are presented to illustrate the methods of inference developed here. Finally, the maximum likelihood, bootstrap and the different Bayes estimates are compared via a Monte Carlo simulation study. 展开更多
关键词 Generalized PARETO (GP) Distribution An ADAPTIVE type-ii Progressive CENSORING Scheme BAYESIAN and Non-Bayesian Estimations Gibbs and Metropolis SAMPLER Bootstrap
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Multiple Type-II截尾样本的统计方法 被引量:2
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作者 费鹤良 《上海师范大学学报(自然科学版)》 2001年第3期6-13,共8页
Multiple Type-II截尾样本或称为带有缺失的定数截尾样本在实际中常会遇到 ,最近十多年来有关它的统计分析方法有了较多的研究结果 ,作者作了综述 。
关键词 缺失定数截尾样本 指数分布 威布尔分布 极大拟然估计 区间估计 MULTIPLE Type-Ⅱ截尾样本
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Growth Optimization, Strain Compensation and Structure Design of InAs/GaSb Type-II Superlattices for Mid-Infrared Imaging 被引量:2
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作者 Yuxin Song Shumin Wang +5 位作者 Carl Asplund Rickard Marcks von Würtemberg Hedda Malm Amir Karim Xiang Lu Jun Shao 《Crystal Structure Theory and Applications》 2013年第2期46-56,共11页
InAs/GaSb type-II superlattce (T2SL) photodetector structures at the MWIR regime were grown by molecular beam epitaxy. The growth temperature and group-V soaking times were optimized with respect to interface and tran... InAs/GaSb type-II superlattce (T2SL) photodetector structures at the MWIR regime were grown by molecular beam epitaxy. The growth temperature and group-V soaking times were optimized with respect to interface and transport quality. Novel strain compensation schemes with insertion of InSb layers were proposed and tested to be efficient to tune the overall strain between tensile and compressive without degradation of interface and optical quality. The effect of the proposed methods is modeled by analytic functions.? Band structure calculations were also carried out for the proposed T2SL structures to assist optimizing sample designs. Single pixel photodiodes with a low dark current were demonstrated. 展开更多
关键词 INAS/GASB type-ii Superlattce Molecular Beam EPITAXY Strain COMPENSATION
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A New Rayleigh Distribution:Properties and Estimation Based on Progressive Type-II Censored Data with an Application 被引量:1
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作者 Ali Algarni Abdullah M.Almarashi 《Computer Modeling in Engineering & Sciences》 SCIE EI 2022年第1期379-396,共18页
In this paper,we propose a new extension of the traditional Rayleigh distribution called the modified Kies Rayleigh distribution.The new distribution contains one scale and one shape parameter and its hazard rate func... In this paper,we propose a new extension of the traditional Rayleigh distribution called the modified Kies Rayleigh distribution.The new distribution contains one scale and one shape parameter and its hazard rate function can be increasing and bathtub-shaped.Some mathematical properties of the new distribution are derived including quantiles and moments.The parameters of modified Kies Rayleigh distribution are estimated based on progressively Type-II censored data.For this purpose,we consider two estimation methods,namely maximum likelihood and maximum product of spacing estimation methods.To compare the efficiency of the proposed estimators,a simulation study is carried out.To show the applicability of the new model as well as the estimation methods,one real data for failure times of software is analyzed.Based on the empirical parts,we can conclude that the proposed model can be considered as a good model in the field of life testing and reliability analysis compared with other competing models. 展开更多
关键词 Rayleigh distribution modified kies family progressive type-ii censored maximum likelihood estimation maximum product of spacing
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EFFECT OF MAGNETIC NANOPARTICLES ON THE MECHANICAL PROPERTIES OF TYPE-II SUPERCONDUCTORS
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作者 Chenguang Huang Huadong Yong Youhe Zhou 《Acta Mechanica Solida Sinica》 SCIE EI CSCD 2014年第1期65-72,共8页
In this paper, the effect of magnetic nanoparticles on the mechanical properties of a type-II superconductor is investigated both theoretically and numerically. Magnetic part of the pinning force associated with the i... In this paper, the effect of magnetic nanoparticles on the mechanical properties of a type-II superconductor is investigated both theoretically and numerically. Magnetic part of the pinning force associated with the interaction between a finite-size spheroidal magnetic inclusion and an Abrikosov vortex is calculated in the London approximation. It is found that the size and shape of magnetic nanoparticles result in different enhancements of vortex pinning in large-k type-II superconductors. Meanwhile, the screening current induced by a magnetic spheroid suffer the action of Lorentz force, which will lead to prestress in the superconductor, so further numerical calculations are needed to explore the interaction between the spheroidal magnetic particle and superconductor. The distribution of displacement, stress and strain in the superconductor are finally obtained. It is shown that different sizes and shapes of nanoparticles also can change the distributions of these quantities. 展开更多
关键词 magnetic nanoparticles spheroidal inclusion pinning force stress distribution type-ii superconductor
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Electronic band structure of a type-II 'W' quantum well calculated by an eight-band k·p model
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作者 迂修 谷永先 +2 位作者 王青 韦欣 陈良惠 《Chinese Physics B》 SCIE EI CAS CSCD 2011年第3期95-100,共6页
In this paper, we present an investigation of type-II 'W' quantum wells for the InAs/Ga1-xInxSb/A1Sb family, where 'W' denotes the conduction profile of the material. We focus our attention on using the eight-band... In this paper, we present an investigation of type-II 'W' quantum wells for the InAs/Ga1-xInxSb/A1Sb family, where 'W' denotes the conduction profile of the material. We focus our attention on using the eight-band k. p model to calculate the band structures within the framework of finite element method. For the sake of clarity, the simulation in this paper is simplified and based on only one period--A1Sb/InAs/Ga1-xInxSb/InAs/A1Sb. The obtained numerical results include the energy levels and wavefunctions of carriers. We discuss the variations of the electronic properties by changing several important parameters, such as the thickness of either InAs or Cal_xInxSb layer and the alloy composition in Ga1-xInxSb separately. In the last part, in order to compare the eight-band k·p model, we recalculate the conduction bands of the 'W' structure using the one-band k·p model and then discuss the difference between the two results, showing that conduction bands are strongly coupled with valence bands in the narrow band gap structure. The in-plane energy dispersions, which illustrate the suppression of the Auger recombination process, are also obtained. 展开更多
关键词 type-ii 'W' quantum well Burt-Foreman Hamiltonian finite element methods
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Long-lived Single Excitons, Trions, and Biexcitons in CdSe/CdTe Type-II Colloidal Quantum Wells
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作者 王俊慧 梁桂杰 吴凯丰 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2017年第6期649-656,I0001,共9页
Light-harvesters with long-lived excited states are desired for efficient solar energy conversion systems. Many solar-to-fuel conversion reactions, such as H2 evolution and CO2 reduction, require multiple sequential e... Light-harvesters with long-lived excited states are desired for efficient solar energy conversion systems. Many solar-to-fuel conversion reactions, such as H2 evolution and CO2 reduction, require multiple sequential electron transfer processes, which leads to a complicated situation that excited states involves not only excitons (electron-hole pairs) but also multi-excitons and charged excitons. While long-lived excitons can be obtained in various systems (e.g., semiconductor nanocrystals), multi-excitons and charged excitons are typically shorted-lived due to nonradiative Auger recombination pathways whereby the recombination energy of an exciton is quickly transferred to the third carrier on a few to hundreds of picoseconds timescale. In this work, we report a study of excitons, trions (an exciton plus an additional charge), and biexcitons in CdSe/CdTe colloidal quantum wells or nanoplatelets. The type- II band alignment effectively separates electrons and holes in space, leading to a single exciton lifetime of 340 ns which is -2 order of magnitudes longer than that in plane CdSe nanoplatelets. More importantly, the electron-hole separation also dramatically slows down Auger decay, giving rise to a trion lifetime of 70 ns and a biexciton lifetime of 11 ns, among the longest values ever reported for colloidal nanocrystals. The long-lived exciton, trion, and biexciton states, combined with the intrinsically strong light-absorption capability of two-dimensional systems, enable the CdSe/CdTe type-II nanoplatelets as promising light harvesters for efficient solar-to-fuel conversion reactions. 展开更多
关键词 Solar energy Excited state lifetime Exciton TRION BIEXCITON type-ii quan-tum wells NANOPLATELETS Auger recombination
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Statistical Inference of Chen Distribution Based on Two Progressive Type-II Censoring Schemes
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作者 Hassan M.Aljohani 《Computers, Materials & Continua》 SCIE EI 2021年第3期2797-2814,共18页
An inverse problemin practical scientific investigations is the process of computing unknown parameters from a set of observations where the observations are only recorded indirectly,such as monitoring and controlling... An inverse problemin practical scientific investigations is the process of computing unknown parameters from a set of observations where the observations are only recorded indirectly,such as monitoring and controlling quality in industrial process control.Linear regression can be thought of as linear inverse problems.In other words,the procedure of unknown estimation parameters can be expressed as an inverse problem.However,maximum likelihood provides an unstable solution,and the problembecomes more complicated if unknown parameters are estimated from different samples.Hence,researchers search for better estimates.We study two joint censoring schemes for lifetime products in industrial process monitoring.In practice,this type of data can be collected in fields such as the medical industry and industrial engineering.In this study,statistical inference for the Chen lifetime products is considered and analyzed to estimate underlying parameters.Maximum likelihood and Bayes’rule are both studied for model parameters.The asymptotic distribution of maximumlikelihood estimators and the empirical distributions obtained withMarkov chainMonte Carlo algorithms are utilized to build the interval estimators.Theoretical results using tables and figures are adopted through simulation studies and verified in an analysis of the lifetime data.We briefly describe the performance of developed methods. 展开更多
关键词 Chen distributions progressive type-ii censoring maximum likelihood mean posterior Bayesian estimation MCMC
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Reliability Analysis of HEE Parameters via Progressive Type-II Censoring with Applications
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作者 Heba S.Mohammed Mazen Nassar +1 位作者 Refah Alotaibi Ahmed Elshahhat 《Computer Modeling in Engineering & Sciences》 SCIE EI 2023年第12期2761-2793,共33页
A new extended exponential lifetime model called Harris extended-exponential(HEE)distribution for data modelling with increasing and decreasing hazard rate shapes has been considered.In the reliability context,researc... A new extended exponential lifetime model called Harris extended-exponential(HEE)distribution for data modelling with increasing and decreasing hazard rate shapes has been considered.In the reliability context,researchers prefer to use censoring plans to collect data in order to achieve a compromise between total test time and/or test sample size.So,this study considers both maximum likelihood and Bayesian estimates of the Harris extended-exponential distribution parameters and some of its reliability indices using a progressive Type-II censoring strategy.Under the premise of independent gamma priors,the Bayesian estimation is created using the squared-error and general entropy loss functions.Due to the challenging form of the joint posterior distribution,to evaluate the Bayes estimates,samples from the full conditional distributions are generated using Markov Chain Monte Carlo techniques.For each unknown parameter,the highest posterior density credible intervals and asymptotic confidence intervals are also determined.Through a simulated study,the usefulness of the various suggested strategies is assessed.The optimal progressive censoring plans are also shown,and various optimality criteria are investigated.Two actual data sets,taken from engineering and veterinary medicine areas,are analyzed to show how the offered point and interval estimators can be used in practice and to verify that the proposed model furnishes a good fit than other lifetimemodels:alpha power exponential,generalized-exponential,Nadarajah-Haghighi,Weibull,Lomax,gamma and exponential distributions.Numerical evaluations revealed that in the presence of progressively Type-II censored data,the Bayes estimation method against the squared-error(symmetric)loss is advised for getting the point and interval estimates of the HEE distribution. 展开更多
关键词 Harris extended-exponential model progressive type-ii censoring RELIABILITY maximum likelihood MCMC techniques Monte Carlo experiments
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Inference on Constant-Partially Accelerated Life Tests for Mixture of Pareto Distributions under Progressive Type-II Censoring
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作者 Tahani A. Abushal Areej M. AL-Zaydi 《Open Journal of Statistics》 2017年第2期323-346,共24页
The main purpose of this paper is to obtain the inference of parameters of heterogeneous population represented by finite mixture of two Pareto (MTP) distributions of the second kind. The constant-partially accelerate... The main purpose of this paper is to obtain the inference of parameters of heterogeneous population represented by finite mixture of two Pareto (MTP) distributions of the second kind. The constant-partially accelerated life tests are applied based on progressively type-II censored samples. The maximum likelihood estimates (MLEs) for the considered parameters are obtained by solving the likelihood equations of the model parameters numerically. The Bayes estimators are obtained by using Markov chain Monte Carlo algorithm under the balanced squared error loss function. Based on Monte Carlo simulation, Bayes estimators are compared with their corresponding maximum likelihood estimators. The two-sample prediction technique is considered to derive Bayesian prediction bounds for future order statistics based on progressively type-II censored informative samples obtained from constant-partially accelerated life testing models. The informative and future samples are assumed to be obtained from the same population. The coverage probabilities and the average interval lengths of the confidence intervals are computed via a Monte Carlo simulation to investigate the procedure of the prediction intervals. Analysis of a simulated data set has also been presented for illustrative purposes. Finally, comparisons are made between Bayesian and maximum likelihood estimators via a Monte Carlo simulation study. 展开更多
关键词 Pareto Distribution Finite Mixtures Constant—Partially ALT Progressive type-ii CENSORING BAYESIAN ESTIMATION Maximum LIKELIHOOD ESTIMATION BAYESIAN PREDICTION the Two-Sample PREDICTION MCMC
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Reanalysis of energy band structure in the type-II quantum wells
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作者 李欣欣 邓震 +4 位作者 江洋 杜春花 贾海强 王文新 陈弘 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第6期75-78,共4页
Band structure analysis holds significant importance for understanding the optoelectronic characteristics of semiconductor structures and exploring their potential applications in practice. For quantum well structures... Band structure analysis holds significant importance for understanding the optoelectronic characteristics of semiconductor structures and exploring their potential applications in practice. For quantum well structures, the energy of carriers in the well splits into discrete energy levels due to the confinement of barriers in the growth direction. However, the discrete energy levels obtained at a fixed wave vector cannot accurately reflect the actual energy band structure. In this work, the band structure of the type-II quantum wells is reanalyzed. When the wave vectors of the entire Brillouin region(corresponding to the growth direction) are taken into account, the quantized energy levels of the carriers in the well are replaced by subbands with certain energy distributions. This new understanding of the energy bands of low-dimensional structures not only helps us to have a deeper cognition of the structure, but also may overturn many viewpoints in traditional band theories and serve as supplementary to the band theory of low-dimensional systems. 展开更多
关键词 energy band structure type-ii quantum wells low-dimensional semiconductors
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AN INTERPOLATION METHOD BY BIVARIATE CUBIC SPLINES WITH C^2 -JOIN ON TYPE-II TRIANGULARS AT A RECTANGULAR DOMAIN
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作者 Tianxiang Zhou Peicai Xuan 《Analysis in Theory and Applications》 2009年第2期125-141,共17页
In this paper, an interpolating method for bivariate cubic splines with C2-join on type-II triangular at a rectangular domain is given, and the approximation degree, inter- polating existence and uniqueness of the cub... In this paper, an interpolating method for bivariate cubic splines with C2-join on type-II triangular at a rectangular domain is given, and the approximation degree, inter- polating existence and uniqueness of the cubic splines are studied. 展开更多
关键词 type-ii triangulation bivariate cubic spline
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Bianchi Type-II, VIII &IX Perfect Fluid Cosmological Models in Brans Dicke Theory of Gravitation
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作者 Velagapudi Uma Maheswara Rao Mandangi Vijaya Santhi 《Journal of Modern Physics》 2011年第10期1222-1228,共7页
Field equations in the presence of perfect fluid distribution are obtained in a scalar tensor theory of gravitation proposed by Brans and Dicke[1] with the aid of Bianchi type-II, VIII & IX metrics. Exact prefect ... Field equations in the presence of perfect fluid distribution are obtained in a scalar tensor theory of gravitation proposed by Brans and Dicke[1] with the aid of Bianchi type-II, VIII & IX metrics. Exact prefect fluid Bianchi type- IX cosmological model is presented since other models doesn’t exist in Brans-Dicke scalar tensor theory of gravitation. Some physical properties of the model are also discussed. 展开更多
关键词 Bianchi type-ii VIII & IX Brans Dicke SCALAR Tensor THEORY Perfect Fluid Distribution
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Long-Period InAs/GaSb Type-II Superlattices for Terahertz Application
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作者 Longlong Li Jun Ni Wen Xu 《Journal of Modern Physics》 2014年第17期1880-1888,共9页
We present a theoretical study on the terahertz (THz) optoelectronic properties of long-period InAs/GaSb type-II super lattices (SLs). The eight-band k·p model is used to calculate the electronic structures of su... We present a theoretical study on the terahertz (THz) optoelectronic properties of long-period InAs/GaSb type-II super lattices (SLs). The eight-band k·p model is used to calculate the electronic structures of such SLs and on the basis of band structures, the Boltzmann equation approach is employed to calculate the optical absorption coefficients for the corresponding SL systems. It is found that long-period InAs/GaSb type-II SLs have a considerable absorption in the THz bandwidth. By examining the dependence of THz absorption coefficient on the InAs/GaSb layer widths, we demonstrate that with a proper choice of InAs/GaSb layer widths, an optimized THz absorption can be achieved. This study is pertinent to the potential application of InAs/GaSb type-II SLs as THz photo detectors. 展开更多
关键词 Long-Period INAS/GASB type-ii SLS THZ OPTOELECTRONIC Properties THZ Band-Gap and Absorption
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ZnSeTe/CdZnSe-based type-II core-shell quantum dots with tunable blue emission
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作者 Muhammad Ramzan Chenhui Wang +4 位作者 Mingrui Liu Peng Huang Jing Li Yin Song Haizheng Zhong 《Nano Research》 2025年第9期39-45,共7页
Blue emissive quantum dots are key materials in emerging light-emitting technologies for display applications.Herein,we report the synthesis of ZnSeTe/CdZnSe-based type-II core–shell quantum dots with a low Cd conten... Blue emissive quantum dots are key materials in emerging light-emitting technologies for display applications.Herein,we report the synthesis of ZnSeTe/CdZnSe-based type-II core–shell quantum dots with a low Cd content of less than 2.5%.By modifying the Cd content of the CdZnSe shell,photoluminescence emission can be tuned from 430 to 510 nm with a full width at half maximum of less than 26 nm.Transient absorption spectra illustrate the charge transfer between the conduction band of ZnSeTe and the conduction band of CdZnSe,as well as the recombination between the valence band of ZnSeTe and the conduction band of CdZnSe.By subsequent growth of ZnSe and ZnS shells,the resulting quantum dots achieved a photoluminescence quantum yield of 95%.We further demonstrate a blue quantum dot light-emitting diode with an emission peak at 467 nm,showing a maximum external quantum efficiency of 5%,a maximum luminance of 10,376 cd·m^(-2),and an extrapolated T95 lifetime of 4.7 h. 展开更多
关键词 quantum dot blue emission type-ii light-emitting diode DISPLAY
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Edge solitons in non-Hermitian photonic lattices with type-II Dirac cones
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作者 Shuang Shen Milivoj R.BeliC +4 位作者 Yiqi Zhang Yongdong Li Tao Wang Zhen-Nan Tian Qi-Dai Chen 《Frontiers of physics》 2025年第4期221-228,共8页
An edge soliton is a localized bound state that arises from the balance between diffraction broadening and nonlinearity-induced self-focusing.It typically resides either at the edge or at the domain wall of a lattice ... An edge soliton is a localized bound state that arises from the balance between diffraction broadening and nonlinearity-induced self-focusing.It typically resides either at the edge or at the domain wall of a lattice system.To the best of our knowledge,most reported edge solitons have been observed in conservative Hermitian systems;whether stable edge solitons can exist in non-Hermitian systems remains an open question.In this work,we utilize a photonic lattice that naturally exhibits type-II Dirac cones and introduce a domain wall by carefully configuring gains and losses at the three sites within each unit cell.Surprisingly,edge states localized at the domain wall can exhibit entirely real propagation constants.Building on these edge states,we demonstrate the existence of edge solitons that can propagate stably over distances significantly exceeding those in the experimental settings adopted in this study.Although these solitons eventually couple with the bulk states and ultimately collapse,they exhibit remarkable resilience.Our findings establish that a domain wall supporting loss-resistant edge solitons,which can also evade the skin effect,is achievable in non-Hermitian systems.This discovery holds promising potential for the development of compact functional optical devices. 展开更多
关键词 type-ii Dirac cones non-Hermitian optics edge solitons
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胰高血糖素样肽1受体激动剂替西帕肽治疗阿尔茨海默病的潜在靶点
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作者 张晓敏 杜朋洋 +1 位作者 张秀萍 薛国芳 《中国组织工程研究》 北大核心 2026年第23期6122-6133,共12页
背景:胰高血糖素样肽1受体激动剂作为神经退行性疾病治疗的新型候选药物,已在阿尔茨海默病临床研究中取得突破性进展,其中索马鲁肽等药物已推进至Ⅲ期临床试验阶段。然而,目前对于该类药物神经保护效应的分子作用机制仍存在显著的知识... 背景:胰高血糖素样肽1受体激动剂作为神经退行性疾病治疗的新型候选药物,已在阿尔茨海默病临床研究中取得突破性进展,其中索马鲁肽等药物已推进至Ⅲ期临床试验阶段。然而,目前对于该类药物神经保护效应的分子作用机制仍存在显著的知识缺口。目的:创新性地整合多组学分析技术与网络药理学方法,系统解析阿尔茨海默病病理相关基因谱系与替西帕肽潜在作用靶点的交集网络,鉴定关键调控基因,并通过体内外实验验证其分子机制。方法:采用多维度研究策略:①基于DisGeNET数据库(整合了多种疾病相关的基因组学数据库)构建阿尔茨海默病差异表达基因谱。②通过PubChem数据库(小分子生物活性数据库)获取替西帕肽结构并筛选潜在靶点。③应用DAVID数据库开展GO功能注释及KEGG通路富集分析。④结合STRING数据库与Cytoscape 3.9.1构建蛋白质互作网络,经拓扑网络分析筛选关键基因。⑤细胞水平验证:将HT22细胞分为对照组、模型组(β-淀粉样蛋白1-42寡聚体处理36 h建立HT22细胞阿尔茨海默病体外模型)、给药组(先以β-淀粉样蛋白1-42寡聚体预处理24 h,再加入替西帕肽共处理12 h),通过Western blot分析血管紧张素Ⅱ2型受体蛋白表达,ELISA检测突触蛋白1、突触后致密物质95等突触功能标志物表达水平。⑥动物实验验证:实验分为3组,对照组为WT型C57BL/6小鼠,腹腔注射生理盐水;模型组为3xTg小鼠(拟阿尔茨海默症小鼠),腹腔注射生理盐水;给药组为3xTg小鼠,腹腔注射20 nmol/L替西帕肽;均为隔日1次,共给药15次。使用水迷宫技术分析阿尔茨海默病模型小鼠的认知行为学改善;使用Western blot定量分析β-淀粉样蛋白(6E10)、磷酸化的Tau蛋白(P-tau-181)的表达情况。结果与结论:①从DisGeNET数据库筛选出阿尔茨海默病相关联的基因,共得到3397个关联基因;根据蛋白关联度筛选出了10个连接度最高的关键基因:AGTR2、NTSR1、NTSR2、GHSR、C5AR1、C3AR1、OPRM1、SSTR2、OPRD1、STAT3;GO富集分析和KEGG通路分析,提示替西帕肽可能通过改善神经受体-配体功能来改善阿尔茨海默病。②细胞实验提示,替西帕肽可能通过改善阿尔茨海默病的突触功能来发挥治疗作用,血管紧张素Ⅱ2型受体可能是替西帕肽治疗阿尔茨海默病的潜在靶点。③动物实验提示,替西帕肽能够改善3xTg小鼠的认知能力,改善3xTg小鼠模型脑内的异常β-淀粉样蛋白沉积和Tau蛋白磷酸化。④结论:揭示血管紧张素Ⅱ2型受体是替西帕肽作用于阿尔茨海默病病理进程的关键分子靶点,替西帕肽可能通过调控血管紧张素Ⅱ2型受体介导的突触功能改善来治疗阿尔茨海默病。 展开更多
关键词 替西帕肽 阿尔茨海默病 网络药理学 血管紧张素Ⅱ2型受体 蛋白互作网络 突触功能
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瑞马唑仑抑制软骨细胞衰老缓解骨关节炎的作用及机制
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作者 柯燕琴 原杰 +1 位作者 贺光辉 张晓玲 《中国组织工程研究》 北大核心 2026年第12期3005-3013,共9页
背景:研发能够阻止甚至逆转关节软骨退化的药物,一直是研究领域的重要科学问题,近年来细胞衰老及靶向调控细胞衰老的策略受到科研人员的关注。目的:探讨瑞马唑仑对小鼠软骨细胞衰老的调控机制,评估瑞马唑仑在骨关节炎中的治疗效果及作... 背景:研发能够阻止甚至逆转关节软骨退化的药物,一直是研究领域的重要科学问题,近年来细胞衰老及靶向调控细胞衰老的策略受到科研人员的关注。目的:探讨瑞马唑仑对小鼠软骨细胞衰老的调控机制,评估瑞马唑仑在骨关节炎中的治疗效果及作用机制。方法:(1)体外细胞实验:培养原代小鼠软骨细胞,使用10 ng/mL白细胞介素1β来诱导骨关节炎软骨细胞模型。通过CCK-8实验确定瑞马唑仑对小鼠软骨细胞的最佳干预浓度。实验设置正常对照组、白细胞介素1β组、瑞马唑仑组,干预24 h后采用Western blot、免疫荧光染色、β-半乳糖苷酶实验检测各组软骨细胞的合成与分解代谢蛋白指标、衰老蛋白指标以及核因子κB信号通路相关指标。(2)动物体内实验:选取8周龄C57/BL6小鼠,随机分为假手术组、内侧半月板失稳组、瑞马唑仑组。在瑞马唑仑治疗4周后,分析小鼠步态,采用番红O-固绿染色观察关节软骨的病理学变化,免疫荧光染色检测Ⅱ型胶原和基质金属蛋白酶13表达水平。结果与结论:(1)体外细胞实验:相对于白细胞介素1β组,使用60μmol/L瑞马唑仑处理后,软骨细胞衰老指标P16、P21表达水平降低,合成代谢指标Ⅱ型胶原和聚集蛋白聚糖表达增加,分解代谢指标基质金属蛋白酶3和基质金属蛋白酶13表达降低以及核因子κB信号通路被抑制。(2)动物体内实验:瑞马唑仑治疗4周后,与内侧半月板失稳组相比,瑞马唑仑组小鼠下肢功能恢复,组织病理学评分改善,Ⅱ型胶原表达增加,基质金属蛋白酶13表达降低。结果表明:瑞马唑仑通过抑制核因子κB信号通路减轻软骨细胞衰老,改善软骨结构,为骨关节炎治疗提供了新的策略。 展开更多
关键词 骨关节炎 瑞马唑仑 软骨细胞 细胞衰老 核因子ΚB信号通路 Ⅱ型胶原 基质金属蛋白酶
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彝药斯赤列二氯甲烷提取物干预骨关节炎模型大鼠的作用及机制
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作者 陆玉春 朱紫陌 +5 位作者 李朝梦 刘桔 江自鲜 李秀芳 王涛 王文静 《中国组织工程研究》 北大核心 2026年第12期3014-3028,共15页
背景:课题组前期研究结果显示彝药斯赤列鲜品甲醇及二氯甲烷提取物具有抗炎、抗氧化、抗血管新生等作用,抗骨关节炎活性明显。目的:探究彝药斯赤列(鲜品)二氯甲烷提取物(fSAW-DCE)对骨关节炎的保护作用及机制。方法:(1)前交叉韧带切断... 背景:课题组前期研究结果显示彝药斯赤列鲜品甲醇及二氯甲烷提取物具有抗炎、抗氧化、抗血管新生等作用,抗骨关节炎活性明显。目的:探究彝药斯赤列(鲜品)二氯甲烷提取物(fSAW-DCE)对骨关节炎的保护作用及机制。方法:(1)前交叉韧带切断法复制大鼠骨关节炎模型,术后连续灌胃斯赤列二氯甲烷提取物干预6周,ELISA检测关节腔液中肿瘤坏死因子α水平,苏木精-伊红染色观察关节软骨病理变化,计算关节软骨矿化程度,Micro CT影像学检查骨赘增生及软骨下骨骨量变化。(2)以小鼠胚胎瘤细胞ATDC5为研究对象,用胰岛素-转铁蛋白-亚硒酸钠和维生素C将其诱导分化为软骨细胞,用白细胞介素1β刺激复制软骨细胞炎症模型,然后给予斯赤列二氯甲烷提取物干预,ELISA试剂盒检测细胞上清液中肿瘤坏死因子α水平,RT-qPCR检测蛋白聚糖及Ⅱ型胶原mRNA表达。(3)转录组测序方法检测斯赤列二氯甲烷提取物干预软骨细胞炎症模型后的差异基因,探究作用机制。结果与结论:(1)斯赤列二氯甲烷提取物可抑制关节炎症,降低关节腔液中肿瘤坏死因子α水平(P<0.05),延缓关节软骨矿质化(P<0.05),减轻关节软骨损伤,维持软骨下骨骨量(P<0.05);(2)斯赤列二氯甲烷提取物可减轻白细胞介素1β诱导的软骨细胞炎症(P<0.05),维持软骨细胞外基质中Ⅱ型胶原mRNA表达水平(P<0.05);(3)转录组学测序及验证结果显示斯赤列二氯甲烷提取物抗骨关节炎的作用与抑制肿瘤坏死因子/核因子κB信号通路的激活有关。 展开更多
关键词 斯赤列 二氯甲烷提取物 骨关节炎 关节软骨 炎症 核因子ΚB 肿瘤坏死因子 转录组学 Ⅱ型胶原
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