Mitochondrial dysfunction and oxidative stress are widely regarded as primary drivers of aging and are associated with several neurodegenerative diseases.The degeneration of motor neurons during aging is a critical pa...Mitochondrial dysfunction and oxidative stress are widely regarded as primary drivers of aging and are associated with several neurodegenerative diseases.The degeneration of motor neurons during aging is a critical pathological factor contributing to the progression of sarcopenia.However,the morphological and functional changes in mitochondria and their interplay in the degeneration of the neuromuscular junction during aging remain poorly understood.A defined systematic search of the Pub Med,Web of Science and Embase databases(last accessed on October 30,2024)was conducted with search terms including'mitochondria','aging'and'NMJ'.Clinical and preclinical studies of mitochondrial dysfunction and neuromuscular junction degeneration during aging.Twentyseven studies were included in this systematic review.This systematic review provides a summary of morphological,functional and biological changes in neuromuscular junction,mitochondrial morphology,biosynthesis,respiratory chain function,and mitophagy during aging.We focus on the interactions and mechanisms underlying the relationship between mitochondria and neuromuscular junctions during aging.Aging is characterized by significant reductions in mitochondrial fusion/fission cycles,biosynthesis,and mitochondrial quality control,which may lead to neuromuscular junction dysfunction,denervation and poor physical performance.Motor nerve terminals that exhibit redox sensitivity are among the first to exhibit abnormalities,ultimately leading to an early decline in muscle strength through impaired neuromuscular junction transmission function.Parg coactivator 1 alpha is a crucial molecule that regulates mitochondrial biogenesis and modulates various pathways,including the mitochondrial respiratory chain,energy deficiency,oxidative stress,and inflammation.Mitochondrial dysfunction is correlated with neuromuscular junction denervation and acetylcholine receptor fragmentation,resulting in muscle atrophy and a decrease in strength during aging.Physical therapy,pharmacotherapy,and gene therapy can alleviate the structural degeneration and functional deterioration of neuromuscular junction by restoring mitochondrial function.Therefore,mitochondria are considered potential targets for preserving neuromuscular junction morphology and function during aging to treat sarcopenia.展开更多
Gastric cancer(GC)is the fifth most prevalent malignancy worldwide and remains a leading cause of cancer-related mortality.Major risk factors for GC include Helicobacter pylori infection,increasing age,high dietary sa...Gastric cancer(GC)is the fifth most prevalent malignancy worldwide and remains a leading cause of cancer-related mortality.Major risk factors for GC include Helicobacter pylori infection,increasing age,high dietary salt intake,and diets deficient in vegetables and fruits.Due to the often subtle and nonspecific early symptoms,coupled with the lack of routine screening programs,a significant proportion of GC cases are diagnosed at advanced stages.The etiology of GC is multifactorial,and diagnosis is confirmed histologically through endoscopic biopsy,followed by staging via computed tomography,positron emission tomography,staging laparoscopy,and endoscopic ultrasound.Treatment strategies typically involve a multidisciplinary approach including chemotherapy,surgical resection,radiotherapy,and emerging immunotherapeutic options.Despite advances in diagnostic and therapeutic modalities,the prognosis of advanced GC remains poor,with high rates of recurrence and metastasis.In recent years,increasing attention has been given to the role of tight junction(TJ)proteins in the pathogenesis and progression of GC.TJ proteins,critical components of epithelial barrier function,have been implicated in various stages of gastric carcinogenesis,from intestinal metaplasia to invasion and metastasis.Infection and inflammation,particularly due to Helicobacter pylori,disrupt TJ integrity,compromising the gastric mucosal barrier and facilitating neoplastic transformation.This review synthesizes current evidence from PubMed,EMBASE,Google Scholar,ScienceDirect,SpringerLink,and other reputable databases to provide a comprehensive overview of the involvement of TJ proteins in GC.By elucidating the molecular interplay between TJ dysregulation and gastric tumorigenesis,this work aims to highlight the potential of TJ proteins as novel diagnostic biomarkers and therapeutic targets in GC management.展开更多
Photocatalysis uses solar energy to convert nitrogen and water directly into ammonia,helping reduce dependence on fossil fuels and offering a way to integrate the nitrogen cycle into a clean energy network.Ohmic junct...Photocatalysis uses solar energy to convert nitrogen and water directly into ammonia,helping reduce dependence on fossil fuels and offering a way to integrate the nitrogen cycle into a clean energy network.Ohmic junctions between metals and semiconductors have demonstrated significant advantages in enhancing stability and reducing carrier recombination,but their application in photocatalytic nitrogen fixation is limited due to the difficulty of work function matching and the complexity of fabrication processes.In this study,density functional theory(DFT) calculations were used to confirm the work function matching between Bi and Bi_(2)Ti_(2)O_(7)(BTO),ensuring the formation of an Ohmic junction.A Bi-Bi_(2)Ti_(2)O_(7)(B-BTO) composite was successfully synthesized via a one-step hydrothermal method,using bismuth nitrate and titanium sulfate as precursors.Compared to pure BTO,the B-BTO heterojunction,driven by dual electron injection from both metal Bi and BTO,significantly increased the ammonia synthesis rate to 686.95 μmol g^(-1)h^(-1),making it the most active nitrogen fixation material among similar pyrochlorebased catalysts to date.The differential charge density calculations,photocurrent(i-t) measurements,and photoluminescence(PL) tests further validate the role of Ohmic contacts in enhancing charge transfer and prolonging carrier lifetimes.This research provides valuable insight into the application of Ohmic junctions in photocatalytic nitrogen fixation and contributes to advancements in this field.展开更多
Applications are limited at present because the currently available ultrasonic motors (USMs) do not provide suffi-ciently high torque and power. The conventional travelling-wave USM needs the bearing to support, which...Applications are limited at present because the currently available ultrasonic motors (USMs) do not provide suffi-ciently high torque and power. The conventional travelling-wave USM needs the bearing to support, which required lubricant. To solve the above problem, a bearingless travelling-wave USM is designed. First, a novel structure of the two-sided USM consisting of a two-sided teeth stator and two disk-type rotors is designed. And the excitation principle of the two-sided travelling-wave USM is analyzed. Then, using ANSYS software, we set up the model of the stator to predict the excitation frequency and modal response of the stator. The shape of the vibration mode was obtained. Last, the load characteristics of the USM are measured using ex-perimental method. The maximum stall torque and the no-load speed were obtained. The results showed that the characteristics of the two-sided USM are better than those of the conventional one-sided USM.展开更多
In this study, forced nonlinear vibration of a circular micro-plate under two-sided electrostatic, two-sided Casimir and external harmonic forces is investigated analytically. For this purpose, at first, von Karman pl...In this study, forced nonlinear vibration of a circular micro-plate under two-sided electrostatic, two-sided Casimir and external harmonic forces is investigated analytically. For this purpose, at first, von Karman plate theory including geometrical nonlinearity is used to obtain the deflection of the micro-plate. Galerkin decomposition method is then employed, and nonlinear ordinary differential equations (ODEs) of motion are determined. A harmonic balance method (HBM) is applied to equations and analytical relation for nonlineaT frequency response (F-R) curves are derived for two categories (including and neglecting Casimir force) separately. The analytical results for three cases:(1) semi-linear vibration;(2) weakly nonlinear vibration;(3) highly non linear vibration, are validated by comparing with the numerical solutio ns. After validation, the effects of the voltage and Casimir force on the natural frequency of two-sided capacitor system are investigated. It is shown that by assuming Casimir force in small gap distances, reduction of the natural frequency is considerable. The influences of the applied voltage, damping, micro-plate thickness and Casimir force on the frequency response curves have been presented too. The results of this study can be useful for modeling circular parallel-plates in nano /microelectromechanical transducers such as microphones and pressure sensors.展开更多
Due to the NP-hardness of the two-sided assembly line balancing （TALB） problem, multiple constraints existing in real applications are less studied, especially when one task is involved with several constraints. In ...Due to the NP-hardness of the two-sided assembly line balancing （TALB） problem, multiple constraints existing in real applications are less studied, especially when one task is involved with several constraints. In this paper, an effective hybrid algorithm is proposed to address the TALB problem with multiple constraints （TALB-MC）. Considering the discrete attribute of TALB-MC and the continuous attribute of the standard teaching-learning-based optimization （TLBO） algorithm, the random-keys method is hired in task permutation representation, for the purpose of bridging the gap between them. Subsequently, a special mechanism for handling multiple constraints is developed. In the mechanism, the directions constraint of each task is ensured by the direction check and adjustment. The zoning constraints and the synchronism constraints are satisfied by teasing out the hidden correlations among constraints. The positional constraint is allowed to be violated to some extent in decoding and punished in cost fimction. Finally, with the TLBO seeking for the global optimum, the variable neighborhood search （VNS） is further hybridized to extend the local search space. The experimental results show that the proposed hybrid algorithm outperforms the late acceptance hill-climbing algorithm （LAHC） for TALB-MC in most cases, especially for large-size problems with multiple constraints, and demonstrates well balance between the exploration and the exploitation. This research proposes an effective and efficient algorithm for solving TALB-MC problem by hybridizing the TLBO and VNS.展开更多
A two-sided assembly line is typically found in plants producing large-sized products. Its advantages over a one-sided line and the difficulties faced in two-sided line balancing problems were discussed. A mathematica...A two-sided assembly line is typically found in plants producing large-sized products. Its advantages over a one-sided line and the difficulties faced in two-sided line balancing problems were discussed. A mathematical model for two-ALB problem was suggested. A modification of the “ranked positional weight” method, namely two-ALB RPW for two-ALB problems was developed. Experiments were carried out to verify the performance of the proposed method and the results show that it is effective in solving two-sided assembly line balancing problems.展开更多
The size and shape of the effective test area are crucial to consider when short-crested waves are created by segmented wavemakers. The range of the effective test area of short-crested waves simulated by two-sided se...The size and shape of the effective test area are crucial to consider when short-crested waves are created by segmented wavemakers. The range of the effective test area of short-crested waves simulated by two-sided segmented wavemakers is analyzed in this paper. The experimental investigation on the wave field distribution of short-crested waves generated by two-sided segmented wavemakers is conducted by using an array of wave gauges. Wave spectra and directional spreading function are analyzed and the results show that when the main direction is at a certain angle with the normal line of wave generators, the wave field of 3D short-crested waves generated by two-sided segmented wavemakers has good spatial uniformity within the model test area. The effective test area can provide good wave environments for seakeeping model tests of various ocean engineering structures in the deep ocean engineering basin.展开更多
In this paper, we consider the two-sided first exit problem for jump diffusion processes having jumps with rational Laplace transforms. We investigate the probabilistic property of conditional memorylessness, and driv...In this paper, we consider the two-sided first exit problem for jump diffusion processes having jumps with rational Laplace transforms. We investigate the probabilistic property of conditional memorylessness, and drive the joint distribution of the first exit time from an interval and the overshoot over the boundary at the exit time.展开更多
In previous research on two-sided matching(TSM)decision,agents’preferences were often given in the form of exact values of ordinal numbers and linguistic phrase term sets.Nowdays,the matching agent cannot perform the...In previous research on two-sided matching(TSM)decision,agents’preferences were often given in the form of exact values of ordinal numbers and linguistic phrase term sets.Nowdays,the matching agent cannot perform the exact evaluation in the TSM situations due to the great fuzziness of human thought and the complexity of reality.Probability hesitant fuzzy sets,however,have grown in popularity due to their advantages in communicating complex information.Therefore,this paper develops a TSM decision-making approach with multi-attribute probability hesitant fuzzy sets and unknown attribute weight information.The agent attribute weight vector should be obtained by using the maximum deviation method and Hamming distance.The probabilistic hesitancy fuzzy information matrix of each agent is then arranged to determine the comprehensive evaluation of two matching agent sets.The agent satisfaction degree is calculated using the technique for order preference by similarity to ideal solution(TOPSIS).Additionally,the multi-object programming technique is used to establish a TSM method with the objective of maximizing the agent satisfaction of two-sided agents,and the matching schemes are then established by solving the built model.The study concludes by providing a real-world supply-demand scenario to illustrate the effectiveness of the proposed method.The proposed method is more flexible than prior research since it expresses evaluation information using probability hesitating fuzzy sets and can be used in scenarios when attribute weight information is unclear.展开更多
In this paper, existence and uniqueness of solution to two-point boundary value for two-sided fractional differential equations involving Caputo fractional derivative is discussed, by means of the Min-Max Theorem.
In this paper,a stable two-sided matching(TSM)method considering the matching intention of agents under a hesitant fuzzy environment is proposed.The method uses a hesitant fuzzy element(HFE)as its basis.First,the HFE ...In this paper,a stable two-sided matching(TSM)method considering the matching intention of agents under a hesitant fuzzy environment is proposed.The method uses a hesitant fuzzy element(HFE)as its basis.First,the HFE preference matrix is transformed into the normalized HFE preference matrix.On this basis,the distance and the projection of the normalized HFEs on positive and negative ideal solutions are calculated.Then,the normalized HFEs are transformed into agent satisfactions.Considering the stable matching constraints,a multiobjective programming model with the objective of maximizing the satisfactions of two-sided agents is constructed.Based on the agent satisfaction matrix,the matching intention matrix of two-sided agents is built.According to the agent satisfaction matrix and matching intention matrix,the comprehensive satisfaction matrix is set up.Furthermore,the multiobjective programming model based on satisfactions is transformed into a multiobjective programming model based on comprehensive satisfactions.Using the G-S algorithm,the multiobjective programming model based on comprehensive satisfactions is solved,and then the best TSM scheme is obtained.Finally,a terminal distribution example is used to verify the feasibility and effectiveness of the proposed method.展开更多
In this paper, we study the logarithmic Sobolev inequalities for two-sided birth-death processes. An estimate of the logarithmic Sobolev constant α for a two-sided birth-death process is obtained by the Hardy-type in...In this paper, we study the logarithmic Sobolev inequalities for two-sided birth-death processes. An estimate of the logarithmic Sobolev constant α for a two-sided birth-death process is obtained by the Hardy-type inequality and a criteria for a is also presented.展开更多
The composition and production technology of the type of hot-rolled steel plate used in two-sided enameling were briefly described. The microstructure and mechanical properties before and after enameling were contrast...The composition and production technology of the type of hot-rolled steel plate used in two-sided enameling were briefly described. The microstructure and mechanical properties before and after enameling were contrastively investigated,and the precipitates in the samples were analyzed using transmission electron microscope and energy dispersive spectrometer. The results show the ferrite grain size of the steel plate after high-temperature enamel firing to be fine,with a large number of TiC and Ti;C;S;precipitates dispersed throughout the ferrite matrix. After two rounds of enamel firing at a temperature range of 800-890 ℃,its yield strength can still reach342 MPa. The results of a hydrogen permeation test show that the hydrogen storage properties of the steel plate are much better than those of ordinary structural carbon steel. A better bubble structure in the enamel layer can be obtained by this steel plate,with no fish-scale defects on the enameled steel-plate surface.展开更多
This article describes some dynamic aspects on dynastic utility incorporating two-sided altruism with an OLG setting. The special case is analyzed where the weights of two-sided altruism are dynamically inconsistent. ...This article describes some dynamic aspects on dynastic utility incorporating two-sided altruism with an OLG setting. The special case is analyzed where the weights of two-sided altruism are dynamically inconsistent. The Bellman equation for two-sided altruism proves to be reduced to one-sided dynamic problem, but the effective discount factor is different only in the current generation. It is shown that a contraction mapping result of value function cannot be achieved in general, and that there can locally exist an infinite number of self-consistent policy functions of the class C＂ with distinct steady states （indeterminacy of self-consistent, differentiable policy functions）.展开更多
Tricellulin,a key tricellular tight junction(TJ)protein,is essential for maintaining the barrier integrity of acinar epithelia against macromolecular passage in salivary glands.This study aims to explore the role and ...Tricellulin,a key tricellular tight junction(TJ)protein,is essential for maintaining the barrier integrity of acinar epithelia against macromolecular passage in salivary glands.This study aims to explore the role and regulatory mechanism of tricellulin in the development of salivary gland hypofunction in Sjögren’s syndrome(SS).Employing a multifaceted approach involving patient biopsies,non-obese diabetic(NOD)mice as a SS model,salivary gland acinar cell-specific tricellulin conditional knockout(TricCKO)mice,and IFN-γ-stimulated salivary gland epithelial cells,we investigated the role of tricellulin in SS-related hyposalivation.Our data revealed diminished levels of tricellulin in salivary glands of SS patients.Similarly,NOD mice displayed a reduction in tricellulin expression from the onset of the disease,concomitant with hyposecretion and an increase in salivary albumin content.Consistent with these findings,TricCKO mice exhibited both hyposecretion and leakage of macromolecular tracers when compared to control animals.Mechanistically,the JAK/STAT1/miR-145 axis was identified as mediating the IFN-γ-induced downregulation of tricellulin.Treatment with AT1001,a TJ sealer,ameliorated epithelial barrier dysfunction,restored tricellulin expression,and consequently alleviated hyposalivation in NOD mice.Importantly,treatment with miR-145 antagomir to specifically recover the expression of tricellulin in NOD mice significantly alleviated hyposalivation and macromolecular leakage.Collectively,we identified that tricellulin deficiency in salivary glands contributed to hyposalivation in SS.Our findings highlight tricellulin as a potential therapeutic target for hyposecretion,particularly in the context of reinforcing epithelial barrier function through preventing leakage of macromolecules in salivary glands.展开更多
Multiferroic tunnel junctions(MFTJs),which combine tunneling magnetoresistance(TMR)and electroresistance(TER)efects,have emerged as key candidates for data storage.Two-dimensional van der Waals(vdW)MFTJs,in particular...Multiferroic tunnel junctions(MFTJs),which combine tunneling magnetoresistance(TMR)and electroresistance(TER)efects,have emerged as key candidates for data storage.Two-dimensional van der Waals(vdW)MFTJs,in particular,are promising spintronic devices for the post-Moore era.However,these vdW MFTJs are typically based on multiferroics composed of ferromagnetic and ferroelectric materials or multilayer magnetic materials with sliding ferroelectricity,which increases device fabrication complexity.In this work,we design a vdW MFTJ using bilayer MoPtGe_(2)S_(6),a material with homologous multiferroicity in each monolayer,combined with symmetric PtTe_(2)electrodes.Using frst-principles calculations based on density functional theory and nonequilibrium Green's functions,we theoretically explore the spin-polarized electronic transport properties of this MFTJ.By controlling the ferroelectric and ferromagnetic polarization directions of bilayer MoPtGe_(2)S_(6),the MFTJ can exhibit six distinct non-volatile resistance states,with maximum TMR(137%)and TER(1943%)ratios.Under biaxial strain,TMR and TER can increase to 265%and 4210%,respectively.The TER ratio also increases to 2186%under a 0.1 V bias voltage.Remarkably,the MFTJ exhibits a pronounced spin-fltering and a signifcant negative diferential resistance efect.These fndings not only highlight the potential of monolayer multiferroic MoPtGe_(2)S_(6)for MFTJs but also ofer valuable theoretical insights for future experimental investigations.展开更多
BACKGROUND The spectrum of gastric submucosal tumors(SMTs)in the upper gastrointestinal system ranges from non-neoplastic to malignant lesions,with gastrointestinal stromal tumors exhibiting inherent malignant potenti...BACKGROUND The spectrum of gastric submucosal tumors(SMTs)in the upper gastrointestinal system ranges from non-neoplastic to malignant lesions,with gastrointestinal stromal tumors exhibiting inherent malignant potential.However,the diagnosis of SMTs remains challenging,and treatment methods,especially for tumors located at the cardia or esophagogastric junction(EGJ),are not well established.Minimally invasive techniques-such as endoscopic submucosal dissection(ESD),submucosal tunneling endoscopic resection(STER),and laparoscopic wedge resection(LWR)-have been developed for these lesions.However,comparative data on their feasibility,safety,and clinical outcomes in these locations remain limited.AIM To compare ESD,STER,and LWR for SMTs at the EGJ or cardia,focusing on procedural feasibility.METHODS This single-center retrospective study included patients with SMTs less than 45 mm from the muscularis propria,growing intraluminally at the EGJ or cardia,and treated with ESD,STER,or LWR between July 2014 and September 2022.The primary outcome was relapse-free survival during follow-up.RESULTS The median age(interquartile range)was 53.0(40.0-57.5),43.0(39.0-57.0),and 56.0(43.0-64.0)years for ESD,STER,and LWR,respectively.The median follow-up time(interquartile range)was 60.0(26.5-66.5),24.0(13.0-38.0),and 35.0(21.0-60.0)months.LWR had the largest tumors(30.0 mm)and the highest rate of high-risk gastrointestinal stromal tumors(68.0%,P<0.001).Tumor recurrence occurred in one LWR patient(4.0%,P=0.600).En bloc and macroscopic resection rates were 100%(P=1.000),but microscopic resection rates differed(P=0.021).Significant minor complications occurred in 5 patients(10.0%),all grade IIIa.Tumor location(cardia/fundus,P=0.006)and prolonged procedure time(P<0.001)were significantly associated with complications.CONCLUSION ESD,STER,and LWR are effective for SMTs at the EGJ and cardia,with minor complications associated with tumor location and procedure time,and comparable recurrence rates.展开更多
Objective:Cytotoxic T lymphocytes(CTLs)play a crucial role in the therapeutic approach to hepatocellular carcinoma(HCC).Recent research has indicated that junctional adhesion molecule-like protein(JAML)enhances the an...Objective:Cytotoxic T lymphocytes(CTLs)play a crucial role in the therapeutic approach to hepatocellular carcinoma(HCC).Recent research has indicated that junctional adhesion molecule-like protein(JAML)enhances the antitumor activity of CD8+T cells.Our study investigates the role of JAML+CD8+T cells in HCC.Methods:We utilized time-of-flight mass cytometry and an orthotopic mouse model of HCC to examine histone modifications in tumor-infiltrating immune cells undergoing immunotherapy.Flow cytometry was used to assess CD4+T cells differentiation and JAML expression in CD8+T cells infiltrating HCC.Correlation analysis revealed a strong positive correlation between lactate dehydrogenase A+(LDHA+)CD4+T cells and JAML+CD8+T cells.Subsequently,we evaluated the therapeutic effects of an agonistic anti-JAML antibody,both alone and combined with immunotherapy.Finally,RNA sequencing was conducted to identify potential regulatory mechanisms.Results:Immunotherapy significantly increased the percentage of CD8+T cells infiltrating HCC and induced histone modifications,such as H3K18 lactylation(H3K18la)in CD4+T cells.Flow cytometry analysis revealed that lactate promotes the differentiation of CD4+T cells into Th1 cells.LDHA,an enzyme that converts pyruvate to lactate,plays a key role in this process.Correlation analysis revealed a strong positive relationship between LDHA+CD4+T cells and JAML+CD8+T cells in patients who responded to immunotherapy.Moreover,high JAML expression in CD8+T cells was associated with a more favorable prognosis.In vivo experiments demonstrated that agonistic anti-JAML antibody therapy reduced tumor volume and significantly prolonged the survival of tumor-bearing mice,independent of the effects of anti-programmed cell death protein ligand-1 antibody(αPD-L1)-mediated immunotherapy.Pathway enrichment analysis further revealed that JAML enhances CTL responses through the oxidative phosphorylation pathway.Conclusions:Activation of JAML enhances CTL responses in HCC treatment,independent ofαPD-L1-mediated immunotherapy,providing a promising strategy for advanced HCC.展开更多
Methane(CH4),the predominant component of natural gas and shale gas,is regarded as a promising carbon feedstock for chemical synthesis[1].However,considering the extreme stability of CH4 molecules,it's quite chall...Methane(CH4),the predominant component of natural gas and shale gas,is regarded as a promising carbon feedstock for chemical synthesis[1].However,considering the extreme stability of CH4 molecules,it's quite challenging in simultaneously achieving high activity and selectivity for target products under mild conditions,especially when synthesizing high-value C2t chemicals such as ethanol[2].The conversion of methane to ethanol by photocatalysis is promising for achieving transformation under ambient temperature and pressure conditions.Currently,the apparent quantum efficiency(AQE)of solar-driven methane-to-ethanol conversion is generally below 0.5%[3,4].Furthermore,the stability of photocatalysts remains inadequate,offering substantial potential for further improvement.展开更多
基金supported by grants from Collaborative Research Fund(Ref:C4032-21GF)General Research Grant(Ref:14114822)+1 种基金Group Research Scheme(Ref:3110146)Area of Excellence(Ref:Ao E/M-402/20)。
文摘Mitochondrial dysfunction and oxidative stress are widely regarded as primary drivers of aging and are associated with several neurodegenerative diseases.The degeneration of motor neurons during aging is a critical pathological factor contributing to the progression of sarcopenia.However,the morphological and functional changes in mitochondria and their interplay in the degeneration of the neuromuscular junction during aging remain poorly understood.A defined systematic search of the Pub Med,Web of Science and Embase databases(last accessed on October 30,2024)was conducted with search terms including'mitochondria','aging'and'NMJ'.Clinical and preclinical studies of mitochondrial dysfunction and neuromuscular junction degeneration during aging.Twentyseven studies were included in this systematic review.This systematic review provides a summary of morphological,functional and biological changes in neuromuscular junction,mitochondrial morphology,biosynthesis,respiratory chain function,and mitophagy during aging.We focus on the interactions and mechanisms underlying the relationship between mitochondria and neuromuscular junctions during aging.Aging is characterized by significant reductions in mitochondrial fusion/fission cycles,biosynthesis,and mitochondrial quality control,which may lead to neuromuscular junction dysfunction,denervation and poor physical performance.Motor nerve terminals that exhibit redox sensitivity are among the first to exhibit abnormalities,ultimately leading to an early decline in muscle strength through impaired neuromuscular junction transmission function.Parg coactivator 1 alpha is a crucial molecule that regulates mitochondrial biogenesis and modulates various pathways,including the mitochondrial respiratory chain,energy deficiency,oxidative stress,and inflammation.Mitochondrial dysfunction is correlated with neuromuscular junction denervation and acetylcholine receptor fragmentation,resulting in muscle atrophy and a decrease in strength during aging.Physical therapy,pharmacotherapy,and gene therapy can alleviate the structural degeneration and functional deterioration of neuromuscular junction by restoring mitochondrial function.Therefore,mitochondria are considered potential targets for preserving neuromuscular junction morphology and function during aging to treat sarcopenia.
文摘Gastric cancer(GC)is the fifth most prevalent malignancy worldwide and remains a leading cause of cancer-related mortality.Major risk factors for GC include Helicobacter pylori infection,increasing age,high dietary salt intake,and diets deficient in vegetables and fruits.Due to the often subtle and nonspecific early symptoms,coupled with the lack of routine screening programs,a significant proportion of GC cases are diagnosed at advanced stages.The etiology of GC is multifactorial,and diagnosis is confirmed histologically through endoscopic biopsy,followed by staging via computed tomography,positron emission tomography,staging laparoscopy,and endoscopic ultrasound.Treatment strategies typically involve a multidisciplinary approach including chemotherapy,surgical resection,radiotherapy,and emerging immunotherapeutic options.Despite advances in diagnostic and therapeutic modalities,the prognosis of advanced GC remains poor,with high rates of recurrence and metastasis.In recent years,increasing attention has been given to the role of tight junction(TJ)proteins in the pathogenesis and progression of GC.TJ proteins,critical components of epithelial barrier function,have been implicated in various stages of gastric carcinogenesis,from intestinal metaplasia to invasion and metastasis.Infection and inflammation,particularly due to Helicobacter pylori,disrupt TJ integrity,compromising the gastric mucosal barrier and facilitating neoplastic transformation.This review synthesizes current evidence from PubMed,EMBASE,Google Scholar,ScienceDirect,SpringerLink,and other reputable databases to provide a comprehensive overview of the involvement of TJ proteins in GC.By elucidating the molecular interplay between TJ dysregulation and gastric tumorigenesis,this work aims to highlight the potential of TJ proteins as novel diagnostic biomarkers and therapeutic targets in GC management.
基金supported by the Natural Science Foundation of China (NSFC,No.52372212)。
文摘Photocatalysis uses solar energy to convert nitrogen and water directly into ammonia,helping reduce dependence on fossil fuels and offering a way to integrate the nitrogen cycle into a clean energy network.Ohmic junctions between metals and semiconductors have demonstrated significant advantages in enhancing stability and reducing carrier recombination,but their application in photocatalytic nitrogen fixation is limited due to the difficulty of work function matching and the complexity of fabrication processes.In this study,density functional theory(DFT) calculations were used to confirm the work function matching between Bi and Bi_(2)Ti_(2)O_(7)(BTO),ensuring the formation of an Ohmic junction.A Bi-Bi_(2)Ti_(2)O_(7)(B-BTO) composite was successfully synthesized via a one-step hydrothermal method,using bismuth nitrate and titanium sulfate as precursors.Compared to pure BTO,the B-BTO heterojunction,driven by dual electron injection from both metal Bi and BTO,significantly increased the ammonia synthesis rate to 686.95 μmol g^(-1)h^(-1),making it the most active nitrogen fixation material among similar pyrochlorebased catalysts to date.The differential charge density calculations,photocurrent(i-t) measurements,and photoluminescence(PL) tests further validate the role of Ohmic contacts in enhancing charge transfer and prolonging carrier lifetimes.This research provides valuable insight into the application of Ohmic junctions in photocatalytic nitrogen fixation and contributes to advancements in this field.
基金Project (No. 50175018) supported by the National Natural ScienceFoundation of China
文摘Applications are limited at present because the currently available ultrasonic motors (USMs) do not provide suffi-ciently high torque and power. The conventional travelling-wave USM needs the bearing to support, which required lubricant. To solve the above problem, a bearingless travelling-wave USM is designed. First, a novel structure of the two-sided USM consisting of a two-sided teeth stator and two disk-type rotors is designed. And the excitation principle of the two-sided travelling-wave USM is analyzed. Then, using ANSYS software, we set up the model of the stator to predict the excitation frequency and modal response of the stator. The shape of the vibration mode was obtained. Last, the load characteristics of the USM are measured using ex-perimental method. The maximum stall torque and the no-load speed were obtained. The results showed that the characteristics of the two-sided USM are better than those of the conventional one-sided USM.
文摘In this study, forced nonlinear vibration of a circular micro-plate under two-sided electrostatic, two-sided Casimir and external harmonic forces is investigated analytically. For this purpose, at first, von Karman plate theory including geometrical nonlinearity is used to obtain the deflection of the micro-plate. Galerkin decomposition method is then employed, and nonlinear ordinary differential equations (ODEs) of motion are determined. A harmonic balance method (HBM) is applied to equations and analytical relation for nonlineaT frequency response (F-R) curves are derived for two categories (including and neglecting Casimir force) separately. The analytical results for three cases:(1) semi-linear vibration;(2) weakly nonlinear vibration;(3) highly non linear vibration, are validated by comparing with the numerical solutio ns. After validation, the effects of the voltage and Casimir force on the natural frequency of two-sided capacitor system are investigated. It is shown that by assuming Casimir force in small gap distances, reduction of the natural frequency is considerable. The influences of the applied voltage, damping, micro-plate thickness and Casimir force on the frequency response curves have been presented too. The results of this study can be useful for modeling circular parallel-plates in nano /microelectromechanical transducers such as microphones and pressure sensors.
基金Supported by National Natural Science Foundation of China(Grant Nos.51275366,50875190,51305311)Specialized Research Fund for the Doctoral Program of Higher Education of China(Grant No.20134219110002)
文摘Due to the NP-hardness of the two-sided assembly line balancing （TALB） problem, multiple constraints existing in real applications are less studied, especially when one task is involved with several constraints. In this paper, an effective hybrid algorithm is proposed to address the TALB problem with multiple constraints （TALB-MC）. Considering the discrete attribute of TALB-MC and the continuous attribute of the standard teaching-learning-based optimization （TLBO） algorithm, the random-keys method is hired in task permutation representation, for the purpose of bridging the gap between them. Subsequently, a special mechanism for handling multiple constraints is developed. In the mechanism, the directions constraint of each task is ensured by the direction check and adjustment. The zoning constraints and the synchronism constraints are satisfied by teasing out the hidden correlations among constraints. The positional constraint is allowed to be violated to some extent in decoding and punished in cost fimction. Finally, with the TLBO seeking for the global optimum, the variable neighborhood search （VNS） is further hybridized to extend the local search space. The experimental results show that the proposed hybrid algorithm outperforms the late acceptance hill-climbing algorithm （LAHC） for TALB-MC in most cases, especially for large-size problems with multiple constraints, and demonstrates well balance between the exploration and the exploitation. This research proposes an effective and efficient algorithm for solving TALB-MC problem by hybridizing the TLBO and VNS.
基金Key Projectof Scientific and TechnologicalCommittee of Shanghai(No.0 3 11110 0 5 )
文摘A two-sided assembly line is typically found in plants producing large-sized products. Its advantages over a one-sided line and the difficulties faced in two-sided line balancing problems were discussed. A mathematical model for two-ALB problem was suggested. A modification of the “ranked positional weight” method, namely two-ALB RPW for two-ALB problems was developed. Experiments were carried out to verify the performance of the proposed method and the results show that it is effective in solving two-sided assembly line balancing problems.
基金financially supported by the National Natural Science Foundation of China(Grant No.51239007)
文摘The size and shape of the effective test area are crucial to consider when short-crested waves are created by segmented wavemakers. The range of the effective test area of short-crested waves simulated by two-sided segmented wavemakers is analyzed in this paper. The experimental investigation on the wave field distribution of short-crested waves generated by two-sided segmented wavemakers is conducted by using an array of wave gauges. Wave spectra and directional spreading function are analyzed and the results show that when the main direction is at a certain angle with the normal line of wave generators, the wave field of 3D short-crested waves generated by two-sided segmented wavemakers has good spatial uniformity within the model test area. The effective test area can provide good wave environments for seakeeping model tests of various ocean engineering structures in the deep ocean engineering basin.
文摘In this paper, we consider the two-sided first exit problem for jump diffusion processes having jumps with rational Laplace transforms. We investigate the probabilistic property of conditional memorylessness, and drive the joint distribution of the first exit time from an interval and the overshoot over the boundary at the exit time.
基金supported by the National Natural Science Foundation in China(Yue Qi,Project No.71861015).
文摘In previous research on two-sided matching(TSM)decision,agents’preferences were often given in the form of exact values of ordinal numbers and linguistic phrase term sets.Nowdays,the matching agent cannot perform the exact evaluation in the TSM situations due to the great fuzziness of human thought and the complexity of reality.Probability hesitant fuzzy sets,however,have grown in popularity due to their advantages in communicating complex information.Therefore,this paper develops a TSM decision-making approach with multi-attribute probability hesitant fuzzy sets and unknown attribute weight information.The agent attribute weight vector should be obtained by using the maximum deviation method and Hamming distance.The probabilistic hesitancy fuzzy information matrix of each agent is then arranged to determine the comprehensive evaluation of two matching agent sets.The agent satisfaction degree is calculated using the technique for order preference by similarity to ideal solution(TOPSIS).Additionally,the multi-object programming technique is used to establish a TSM method with the objective of maximizing the agent satisfaction of two-sided agents,and the matching schemes are then established by solving the built model.The study concludes by providing a real-world supply-demand scenario to illustrate the effectiveness of the proposed method.The proposed method is more flexible than prior research since it expresses evaluation information using probability hesitating fuzzy sets and can be used in scenarios when attribute weight information is unclear.
文摘In this paper, existence and uniqueness of solution to two-point boundary value for two-sided fractional differential equations involving Caputo fractional derivative is discussed, by means of the Min-Max Theorem.
基金supported by the National Natural Science Foundation of China (Grant No.71861015)the Humanities and Social Science Foundation of the Ministry of Education of China (Grant No.18YJA630047)the Distinguished Young Scholar Talent of Jiangxi Province (Grant No.20192BCBL23008).
文摘In this paper,a stable two-sided matching(TSM)method considering the matching intention of agents under a hesitant fuzzy environment is proposed.The method uses a hesitant fuzzy element(HFE)as its basis.First,the HFE preference matrix is transformed into the normalized HFE preference matrix.On this basis,the distance and the projection of the normalized HFEs on positive and negative ideal solutions are calculated.Then,the normalized HFEs are transformed into agent satisfactions.Considering the stable matching constraints,a multiobjective programming model with the objective of maximizing the satisfactions of two-sided agents is constructed.Based on the agent satisfaction matrix,the matching intention matrix of two-sided agents is built.According to the agent satisfaction matrix and matching intention matrix,the comprehensive satisfaction matrix is set up.Furthermore,the multiobjective programming model based on satisfactions is transformed into a multiobjective programming model based on comprehensive satisfactions.Using the G-S algorithm,the multiobjective programming model based on comprehensive satisfactions is solved,and then the best TSM scheme is obtained.Finally,a terminal distribution example is used to verify the feasibility and effectiveness of the proposed method.
基金the National Natural Science Foundation of China(10271091)
文摘In this paper, we study the logarithmic Sobolev inequalities for two-sided birth-death processes. An estimate of the logarithmic Sobolev constant α for a two-sided birth-death process is obtained by the Hardy-type inequality and a criteria for a is also presented.
文摘The composition and production technology of the type of hot-rolled steel plate used in two-sided enameling were briefly described. The microstructure and mechanical properties before and after enameling were contrastively investigated,and the precipitates in the samples were analyzed using transmission electron microscope and energy dispersive spectrometer. The results show the ferrite grain size of the steel plate after high-temperature enamel firing to be fine,with a large number of TiC and Ti;C;S;precipitates dispersed throughout the ferrite matrix. After two rounds of enamel firing at a temperature range of 800-890 ℃,its yield strength can still reach342 MPa. The results of a hydrogen permeation test show that the hydrogen storage properties of the steel plate are much better than those of ordinary structural carbon steel. A better bubble structure in the enamel layer can be obtained by this steel plate,with no fish-scale defects on the enameled steel-plate surface.
文摘This article describes some dynamic aspects on dynastic utility incorporating two-sided altruism with an OLG setting. The special case is analyzed where the weights of two-sided altruism are dynamically inconsistent. The Bellman equation for two-sided altruism proves to be reduced to one-sided dynamic problem, but the effective discount factor is different only in the current generation. It is shown that a contraction mapping result of value function cannot be achieved in general, and that there can locally exist an infinite number of self-consistent policy functions of the class C＂ with distinct steady states （indeterminacy of self-consistent, differentiable policy functions）.
基金supported by the National Natural Science Foundation of China(grants 31972908,81991500,81991502,and 32030010)Beijing Natural Science Foundation(grant 7202082).
文摘Tricellulin,a key tricellular tight junction(TJ)protein,is essential for maintaining the barrier integrity of acinar epithelia against macromolecular passage in salivary glands.This study aims to explore the role and regulatory mechanism of tricellulin in the development of salivary gland hypofunction in Sjögren’s syndrome(SS).Employing a multifaceted approach involving patient biopsies,non-obese diabetic(NOD)mice as a SS model,salivary gland acinar cell-specific tricellulin conditional knockout(TricCKO)mice,and IFN-γ-stimulated salivary gland epithelial cells,we investigated the role of tricellulin in SS-related hyposalivation.Our data revealed diminished levels of tricellulin in salivary glands of SS patients.Similarly,NOD mice displayed a reduction in tricellulin expression from the onset of the disease,concomitant with hyposecretion and an increase in salivary albumin content.Consistent with these findings,TricCKO mice exhibited both hyposecretion and leakage of macromolecular tracers when compared to control animals.Mechanistically,the JAK/STAT1/miR-145 axis was identified as mediating the IFN-γ-induced downregulation of tricellulin.Treatment with AT1001,a TJ sealer,ameliorated epithelial barrier dysfunction,restored tricellulin expression,and consequently alleviated hyposalivation in NOD mice.Importantly,treatment with miR-145 antagomir to specifically recover the expression of tricellulin in NOD mice significantly alleviated hyposalivation and macromolecular leakage.Collectively,we identified that tricellulin deficiency in salivary glands contributed to hyposalivation in SS.Our findings highlight tricellulin as a potential therapeutic target for hyposecretion,particularly in the context of reinforcing epithelial barrier function through preventing leakage of macromolecules in salivary glands.
基金supported by the National Key R&D Program of China(Grant No.2022YFB3505301)the National Key R&D Program of Shanxi Province(Grant No.202302050201014)+1 种基金the National Natural Science Foundation of China(Grant No.12304148)the Natural Science Basic Research Program of Shanxi Province(Grant No.202203021222219)。
文摘Multiferroic tunnel junctions(MFTJs),which combine tunneling magnetoresistance(TMR)and electroresistance(TER)efects,have emerged as key candidates for data storage.Two-dimensional van der Waals(vdW)MFTJs,in particular,are promising spintronic devices for the post-Moore era.However,these vdW MFTJs are typically based on multiferroics composed of ferromagnetic and ferroelectric materials or multilayer magnetic materials with sliding ferroelectricity,which increases device fabrication complexity.In this work,we design a vdW MFTJ using bilayer MoPtGe_(2)S_(6),a material with homologous multiferroicity in each monolayer,combined with symmetric PtTe_(2)electrodes.Using frst-principles calculations based on density functional theory and nonequilibrium Green's functions,we theoretically explore the spin-polarized electronic transport properties of this MFTJ.By controlling the ferroelectric and ferromagnetic polarization directions of bilayer MoPtGe_(2)S_(6),the MFTJ can exhibit six distinct non-volatile resistance states,with maximum TMR(137%)and TER(1943%)ratios.Under biaxial strain,TMR and TER can increase to 265%and 4210%,respectively.The TER ratio also increases to 2186%under a 0.1 V bias voltage.Remarkably,the MFTJ exhibits a pronounced spin-fltering and a signifcant negative diferential resistance efect.These fndings not only highlight the potential of monolayer multiferroic MoPtGe_(2)S_(6)for MFTJs but also ofer valuable theoretical insights for future experimental investigations.
文摘BACKGROUND The spectrum of gastric submucosal tumors(SMTs)in the upper gastrointestinal system ranges from non-neoplastic to malignant lesions,with gastrointestinal stromal tumors exhibiting inherent malignant potential.However,the diagnosis of SMTs remains challenging,and treatment methods,especially for tumors located at the cardia or esophagogastric junction(EGJ),are not well established.Minimally invasive techniques-such as endoscopic submucosal dissection(ESD),submucosal tunneling endoscopic resection(STER),and laparoscopic wedge resection(LWR)-have been developed for these lesions.However,comparative data on their feasibility,safety,and clinical outcomes in these locations remain limited.AIM To compare ESD,STER,and LWR for SMTs at the EGJ or cardia,focusing on procedural feasibility.METHODS This single-center retrospective study included patients with SMTs less than 45 mm from the muscularis propria,growing intraluminally at the EGJ or cardia,and treated with ESD,STER,or LWR between July 2014 and September 2022.The primary outcome was relapse-free survival during follow-up.RESULTS The median age(interquartile range)was 53.0(40.0-57.5),43.0(39.0-57.0),and 56.0(43.0-64.0)years for ESD,STER,and LWR,respectively.The median follow-up time(interquartile range)was 60.0(26.5-66.5),24.0(13.0-38.0),and 35.0(21.0-60.0)months.LWR had the largest tumors(30.0 mm)and the highest rate of high-risk gastrointestinal stromal tumors(68.0%,P<0.001).Tumor recurrence occurred in one LWR patient(4.0%,P=0.600).En bloc and macroscopic resection rates were 100%(P=1.000),but microscopic resection rates differed(P=0.021).Significant minor complications occurred in 5 patients(10.0%),all grade IIIa.Tumor location(cardia/fundus,P=0.006)and prolonged procedure time(P<0.001)were significantly associated with complications.CONCLUSION ESD,STER,and LWR are effective for SMTs at the EGJ and cardia,with minor complications associated with tumor location and procedure time,and comparable recurrence rates.
基金funded by the Major Research Plan of the National Natural Science Foundation of China(No.92159202)the National Key Research and Development Program of China(No.2021YFA1100500)+1 种基金the Leading Innovation Team Project of Hangzhou Medical College(No.CXLJ202401)the Key Research and Development Plan of Zhejiang Provincial Department of Science and Technology(No.2024C03051)。
文摘Objective:Cytotoxic T lymphocytes(CTLs)play a crucial role in the therapeutic approach to hepatocellular carcinoma(HCC).Recent research has indicated that junctional adhesion molecule-like protein(JAML)enhances the antitumor activity of CD8+T cells.Our study investigates the role of JAML+CD8+T cells in HCC.Methods:We utilized time-of-flight mass cytometry and an orthotopic mouse model of HCC to examine histone modifications in tumor-infiltrating immune cells undergoing immunotherapy.Flow cytometry was used to assess CD4+T cells differentiation and JAML expression in CD8+T cells infiltrating HCC.Correlation analysis revealed a strong positive correlation between lactate dehydrogenase A+(LDHA+)CD4+T cells and JAML+CD8+T cells.Subsequently,we evaluated the therapeutic effects of an agonistic anti-JAML antibody,both alone and combined with immunotherapy.Finally,RNA sequencing was conducted to identify potential regulatory mechanisms.Results:Immunotherapy significantly increased the percentage of CD8+T cells infiltrating HCC and induced histone modifications,such as H3K18 lactylation(H3K18la)in CD4+T cells.Flow cytometry analysis revealed that lactate promotes the differentiation of CD4+T cells into Th1 cells.LDHA,an enzyme that converts pyruvate to lactate,plays a key role in this process.Correlation analysis revealed a strong positive relationship between LDHA+CD4+T cells and JAML+CD8+T cells in patients who responded to immunotherapy.Moreover,high JAML expression in CD8+T cells was associated with a more favorable prognosis.In vivo experiments demonstrated that agonistic anti-JAML antibody therapy reduced tumor volume and significantly prolonged the survival of tumor-bearing mice,independent of the effects of anti-programmed cell death protein ligand-1 antibody(αPD-L1)-mediated immunotherapy.Pathway enrichment analysis further revealed that JAML enhances CTL responses through the oxidative phosphorylation pathway.Conclusions:Activation of JAML enhances CTL responses in HCC treatment,independent ofαPD-L1-mediated immunotherapy,providing a promising strategy for advanced HCC.
基金the support from the National Natural Science Foundation of China(52202306)Program from Guangdong Introducing Innovative and Entrepreneurial Teams(2019ZT08L101 and RCTDPT-2020-001)+1 种基金Shenzhen Key Laboratory of Eco-materials and Renewable Energy(ZDSYS20200922160400001)the Provincial Talent Plan of Guangdong(2023TB0012).
文摘Methane(CH4),the predominant component of natural gas and shale gas,is regarded as a promising carbon feedstock for chemical synthesis[1].However,considering the extreme stability of CH4 molecules,it's quite challenging in simultaneously achieving high activity and selectivity for target products under mild conditions,especially when synthesizing high-value C2t chemicals such as ethanol[2].The conversion of methane to ethanol by photocatalysis is promising for achieving transformation under ambient temperature and pressure conditions.Currently,the apparent quantum efficiency(AQE)of solar-driven methane-to-ethanol conversion is generally below 0.5%[3,4].Furthermore,the stability of photocatalysts remains inadequate,offering substantial potential for further improvement.
