The purpose of this study is to characterize the re-epithelialization of wound healing in canine prostatic urethra and to evaluate the effect of this re-epithelialization way after two-micron laser resection of the pr...The purpose of this study is to characterize the re-epithelialization of wound healing in canine prostatic urethra and to evaluate the effect of this re-epithelialization way after two-micron laser resection of the prostate （TmLRP）. TmLRP and partial bladder neck mucosa were performed in 15 healthy adult male crossbred canines. Wound specimens were harvested at 3 days, and 1, 2, 3, and 4 weeks after operation, respectively. The histopathologic characteristics were observed by hematoxylin and eosin staining. The expression of cytokeratin 14 （CK14）, CK5, CK18, synaptophysin （Syn）, chromogranin A （CgA）, uroplakin, transforming growth factor-β1 （TGF-β1）, and TGF-β type Ⅱ receptor in prostatic urethra wound were examined by immunohistochemistry and real-time polymerase chain reaction, respectively. Van Gieson staining was performed to determine the expression of collagen fibers in prostatic urethra and bladder neck would. The results showed that the re-epithelialization of the prostatic urethra resulted from the mobilization of proliferating epithelial cells from residual prostate tissue under the wound. The proliferating cells expressed CK14, CK5, but not CK18, Syn, and CgA and re-epithelialize expressed uroplakin since 3 weeks. There were enhanced TGF-β1 and TGF-β type Ⅱ receptor expression in proliferating cells and regenerated cells, which correlated with specific phases of re-epithelialization. Compared with the re-epithelialization of the bladder neck, re-epithelialization of canine prostatic urethra was faster, and the expression of collagen fibers was relatively low. In conclusion, re-epithelialization in canine prostatic urethra resulted from prostate basal cells after TmLRP and this re-epithelialization way may represent the ideal healing method from anatomic repair to functional recovery after injury.展开更多
目的探讨2μm激光治疗早期输尿管癌的安全性和可行性。方法应用2μm激光治疗25例早期输尿管癌患者,对手术时间、安全性及术后疗效做出评估。结果所有例手术均成功完成且无并发症发生。术后组织学病理均为尿路上皮癌,20例为非浸润性尿路...目的探讨2μm激光治疗早期输尿管癌的安全性和可行性。方法应用2μm激光治疗25例早期输尿管癌患者,对手术时间、安全性及术后疗效做出评估。结果所有例手术均成功完成且无并发症发生。术后组织学病理均为尿路上皮癌,20例为非浸润性尿路上皮癌（低级别）,3例为非浸润性尿路上皮癌（高级别）,1例浸润性尿路上皮癌（高级别）。手术时间28～45 min,平均38 min。术中出血量5～10 m L,平均7 m L。术后平均随访12个月（8～18个月）,仅1例术后6个月后膀胱内复发,患者接受了2微米激光膀胱肿瘤切除术。结论2μm激光治疗早期输尿管癌安全、有效、可行,远期疗效仍需进一步观察。展开更多
文摘The purpose of this study is to characterize the re-epithelialization of wound healing in canine prostatic urethra and to evaluate the effect of this re-epithelialization way after two-micron laser resection of the prostate （TmLRP）. TmLRP and partial bladder neck mucosa were performed in 15 healthy adult male crossbred canines. Wound specimens were harvested at 3 days, and 1, 2, 3, and 4 weeks after operation, respectively. The histopathologic characteristics were observed by hematoxylin and eosin staining. The expression of cytokeratin 14 （CK14）, CK5, CK18, synaptophysin （Syn）, chromogranin A （CgA）, uroplakin, transforming growth factor-β1 （TGF-β1）, and TGF-β type Ⅱ receptor in prostatic urethra wound were examined by immunohistochemistry and real-time polymerase chain reaction, respectively. Van Gieson staining was performed to determine the expression of collagen fibers in prostatic urethra and bladder neck would. The results showed that the re-epithelialization of the prostatic urethra resulted from the mobilization of proliferating epithelial cells from residual prostate tissue under the wound. The proliferating cells expressed CK14, CK5, but not CK18, Syn, and CgA and re-epithelialize expressed uroplakin since 3 weeks. There were enhanced TGF-β1 and TGF-β type Ⅱ receptor expression in proliferating cells and regenerated cells, which correlated with specific phases of re-epithelialization. Compared with the re-epithelialization of the bladder neck, re-epithelialization of canine prostatic urethra was faster, and the expression of collagen fibers was relatively low. In conclusion, re-epithelialization in canine prostatic urethra resulted from prostate basal cells after TmLRP and this re-epithelialization way may represent the ideal healing method from anatomic repair to functional recovery after injury.
文摘目的探讨2μm激光治疗早期输尿管癌的安全性和可行性。方法应用2μm激光治疗25例早期输尿管癌患者,对手术时间、安全性及术后疗效做出评估。结果所有例手术均成功完成且无并发症发生。术后组织学病理均为尿路上皮癌,20例为非浸润性尿路上皮癌（低级别）,3例为非浸润性尿路上皮癌（高级别）,1例浸润性尿路上皮癌（高级别）。手术时间28～45 min,平均38 min。术中出血量5～10 m L,平均7 m L。术后平均随访12个月（8～18个月）,仅1例术后6个月后膀胱内复发,患者接受了2微米激光膀胱肿瘤切除术。结论2μm激光治疗早期输尿管癌安全、有效、可行,远期疗效仍需进一步观察。
