The insecure problems of seed production have seriously hampered the healthy and sustainable development of two-line hybrid rice.The safety issues on seed production of two-line hybrid rice and current research situat...The insecure problems of seed production have seriously hampered the healthy and sustainable development of two-line hybrid rice.The safety issues on seed production of two-line hybrid rice and current research situation were pointed out in this paper.The three main reasons for unsafety in seed production of twoline hybrid rice were unsuitable site selection,high critical sterility-inducing temperature and the drift of critical temperature.In this paper,strategies and measures were put forward based on many years of practice.It could minimize the risk in seed production of two-line hybrid rice by selecting dual-purpose genic male sterile line with lower critical sterility-inducing temperature and long lower temperature resistant time.Based on the climate data and climatic demands of the ＂three safe-periods＂ in seed production,a new idea for determining appropriate bases and periods for seed production was proposed by using computer technology,which solved the aimless selection of sites and periods for the seed production of two-line hybrid rice.Besides,we established a system of single plant selection and identification method and original seed propagation with cyclic cold water,which could reduce the generation number of original seed propagation in seed production and avoid the drift of critical sterility-inducing temperature.This paper improved the seed production safety in the three aspects of seed nature,seed source and seed production site.展开更多
Seeds of two-line sterile rice cultivars Zhu 1S and Lu 18S were carried into space by ＂Shijian 8＂ breeding recoverable satellite, then planted in ground. Mutagenic effects from space induction were compared with tho...Seeds of two-line sterile rice cultivars Zhu 1S and Lu 18S were carried into space by ＂Shijian 8＂ breeding recoverable satellite, then planted in ground. Mutagenic effects from space induction were compared with those from y-irradiation and complex processing of space induction and y-irradiation. The results showed that agronomic effect was stimulated in Mo progenies of the two-line sterile rice varieties treated by space flight, and their radiosensitivities to the irradiation of space flight performed non-sensitive. The order of mutation frequency was determined to be SP ＋ γ 〉 γ 〉 SP in M2 generation. And a series of mutated elites（individuals） were screened. Physiological indices of mutants screened like the activity of protective enzymes were measured to explore the physiological and biochemical basis of biological effect in space environment to two-line sterile rice. The results of this study show that space mutation breeding is an effective novel mean for breeding.展开更多
[Objective] The aim was to research effects of N quantity on grain-filling characters of two-line hybrid rice cultivars with large ears. [Method] Peiza 67 and 88, two-line hybridized rice with large ears, were made us...[Objective] The aim was to research effects of N quantity on grain-filling characters of two-line hybrid rice cultivars with large ears. [Method] Peiza 67 and 88, two-line hybridized rice with large ears, were made use of to study on effects of N fertilizer in different quantities （LN： 90 kg/hm2;MN： 180 kg/hm2;HN： 270 kg/hm2） on plumpness and grain-filling characters. [Result] When N fertilizers were excessive, for inferior grains, grain-filling rate decreased and grain-filling time extended, resulting in plumpness decline after degradation of leaves＇ function. When N fertilizers were inadequate, maximal and average grain-filling rates decreased and the differences between superior and inferior grains in grain-filling rate increased, leading to decline of grain＇s weight and plumpness degree. On the other hand, quantity of N fertilizers had little effect on superior grains in plumpness. [Conclusion] The research provided references for reasonable use of N fertilizer and improvement of rice yield and N use.展开更多
The breeding processes, characteristics and comprehensive characters of two-line hybrid rice Yanliangyou 2208 and its parents （Yan 220S and Yanhui 888） taking part in the national rice regional test of southern rice...The breeding processes, characteristics and comprehensive characters of two-line hybrid rice Yanliangyou 2208 and its parents （Yan 220S and Yanhui 888） taking part in the national rice regional test of southern rice regions were introduced. Yanliangyou 2208 was approved by the National Crop Variety Appraisal Committee in 2013. The achievement has been transformed successfully and has been applied in production. It has good development and promotion prospects.展开更多
[Objective]The research aimed at constructing an accumulated temperature effect model for fertility alteration of two-line hybrid rice P88S.[Method]Analyze the experiment datas of fertility alteration of P88S in Haina...[Objective]The research aimed at constructing an accumulated temperature effect model for fertility alteration of two-line hybrid rice P88S.[Method]Analyze the experiment datas of fertility alteration of P88S in Hainan from 2005 to 2006.[Result]The fertilities of P88S with temperatures ranging from 26 to 28 ℃ were determined by the absolute values and the accumulation effects of the temperature.An accumulation effect model of temperatures based on the bistable state was obtained.[Conclusion]The temperature asymmetry of fertility alteration for two-line sterility line in spring and autumn can be elucidated effectively using the accumulated temperature effect model,namely,alternating from fertile to sterile requires higher temperatures,and alternating from sterile to fertile requires lower temperatures.展开更多
The main problems about research and application of two-line hybrid rice were reviewed, including the confusing nomenclature and male sterile lines classification, the unclear characteristics of photoperiod and temper...The main problems about research and application of two-line hybrid rice were reviewed, including the confusing nomenclature and male sterile lines classification, the unclear characteristics of photoperiod and temperature responses and the unsuitable site selection for male sterile line and hybrid dce seed production. In order to efficiently and accurately use dual-purpose genic male sterile lines, four types, including PTGMS （photo-thermo-sensitive genic male sterile rice）, TGMS （thermo-sensitive genic male sterile rice）, reverse PTGMS and reverse TGMS, were proposed. A new idea for explaining the mechanism of sterility in dual-purpose hybrid rice was proposed. The transition from sterile to fertile was involved in the cooperative regulation of major-effect sterile genes and photoperiod and/or temperature sensitive ones. The minor-effect genes with accumulative effect on sterility were important factors that affected the critical temperature of sterility transfer. In order to make better use of dual-purpose lines, the characterization of responses to photoperiod and temperature of PTGMS should be made and the identification method for the characterization of photoperiod and temperature responses of PTGMS should also be put forward. The optimal ecological site for seed production could be determined according to the historical climate data and the requirements for the meteorological conditions during the different periods of seed production.展开更多
By inducing frequency, intensity and duration of lower temperature in the middle and last ten-day periods in August in the rice-growing areas of southern China, increasing temperature for safe seed production was defi...By inducing frequency, intensity and duration of lower temperature in the middle and last ten-day periods in August in the rice-growing areas of southern China, increasing temperature for safe seed production was defined as 2℃. During the sensitive period of fertility, characters of panicle height and canopy structure of TGMS rice, Pei＇ai64S, were measured. Results showed that temperature changes caused by irrigation in fields were below 40 cm of rice plant, and heating effect was significant at 20 cm when the temperature was increased by 3.1 ℃. For the present study, the following irrigation techniques were put forth： the water depth of 15-20 cm, current water used, irrigating after 17：00 and bailing at 10：00 in sunny or cloudy weather, irrigating on whole day, in shady or rainy weather, increasing inflows and outflows in large fields. In the present experiment, pollen fertility and self-fertilized seed setting rate accepted that the techniques were feasible and effective for against lower temperature and safeguarding seed production of two-line hybrid rice.展开更多
This study was designed to elucidate the grain filling characteristics and the causes of poor grain plumpness of some two-line hybrid rice combinations and their hormonal mechanism. Six varieties, including three two-...This study was designed to elucidate the grain filling characteristics and the causes of poor grain plumpness of some two-line hybrid rice combinations and their hormonal mechanism. Six varieties, including three two-line hybrid rice combinations, that show differences in seed-setting and grain filling, were used. And the contents of starch, sucrose, zeatin （Z） ＋ zeatin riboside （ZR）, indole-3-acetic acid （IAA）, and abscisic acid （ABA）, the ethylene evolution rate, activities of sucrose synthase （SuSase） and starch synthase （StSase） in grains, the seed-setting and grain filling rate were investigated. The correlations amongst these were analyzed. The results showed that the poor grain filling of two-line hybrids was mainly attributed to the higher unfilled grain rate and the lower filling degree of inferior grains. During the early and mid grain filling periods, the sucrose content in inferior grains was greater than that in superior grains for the combinations with poor grain filling, indicating that the substrate concentration was not the principal factor for their slow grain filling and poor grain plumpness of the inferior grains of two-line hybrids. Z ＋ ZR, IAA, and ABA in superior grains were obviously greater than those in inferior grains at early grain filling stage. The maximum and mean contents of Z ＋ ZR, IAA, and ABA were positively very significantly correlated with the maximum and mean grain-filling rate, filling degree, and grain weight. The evolution rate of ethylene was greater in inferior grains than in superior grains and greater for the combinations with poor grain plumpness than those with good grain plumpness at the early or mid filling stages. The evolution rate of ethylene was negatively and significantly correlated with the grain filling rate, the grain filling degree, and the grain weight. Spraying ethephon （ethylene-releasing agent） at the early grain filling stage increased the evolution rate of ethylene, reduced the ABA content and activities of SuSase and StSase, and decreased the grain filling degree and the grain weight. The results were reversed when cobatous nitrate （an inhibitor of ethylene synthesis） was applied. The results suggested that the hormones and their balance play a role in the regulation of grain filling and enzymatic activities, and the poor grain filling is attributed to the low contents of Z ＋ ZR, IAA, and ABA, and the high evolution rate of ethylene in the inferior grains of some two-line hybrid rice combinations. The results suggested that hormones play important roles in the grain filling of some two-line hybrid rice combinations, and their filling degree can be improved by regulating the hormonal contents.展开更多
[Objective] The aim of this study was to explore the inheritance pattern of the resistance of two-line hybrid rice to false smut. [Method] A moderate susceptible TGMS line 33S as the female parent, was crossed with 30...[Objective] The aim of this study was to explore the inheritance pattern of the resistance of two-line hybrid rice to false smut. [Method] A moderate susceptible TGMS line 33S as the female parent, was crossed with 30 susceptible restorer lines and 23 resistant restorer lines. Then false smut resistance of the hybrid F1 and parents were identified. [Result] All F1 of 33S crossed with 30 susceptible re- storer lines were susceptible to rice false smut. The F1 of 33S crossed with 23 re- sistant restorer lines showed dominant, incompletely dominant or recessive inheri- tance. Dominant and incompletely dominant inheritance accounted for 87.0%. The diseased panicle rate, disease index, average diseased grains per panicle, and the highest diseased grains per panicle of F1 shared highly significant positive correla- tions with corresponding indicators of restorer lines. [Conclusion] This study provides references for genetic improvement of two-line hybrid rice.展开更多
The genetic diversity of 50 parents or improved lines of two-line medium Indica hybrid rice were analyzed based on 48 pairs of SSR markers. The results showed that the clustering results were highly accordant with the...The genetic diversity of 50 parents or improved lines of two-line medium Indica hybrid rice were analyzed based on 48 pairs of SSR markers. The results showed that the clustering results were highly accordant with the parental pedigree information of the tested materials. The male sterile lines and the restoring lines were clustered into 2 distinct groups （Ⅰ and Ⅱ）, respectively. There were no significant difference in intra-group average genetic distances （GD） between the 2 groups. Most of the male sterile lines from Hunan Longping High-tech Seed Science Research Institute Co., Ltd. were grouped into a single subgroup （Ⅰ-4） under the male sterile group with the largest average GD compared with the rest male sterile subgroups. Huazhan derived lines and Guangzhou inbred lines were founded to be independent of 9311 and its derivatives or closely-related lines. Meanwhile, they have obviously been differentiated into 2 subgroups （Ⅱ-2 and Ⅱ-3）. The lines derived from Huazhan （or genetic closely related lines） showed a genetic homogenization phenomenon. Therefore, the Huazhan-derived lines should be further improved by the utilization of germplasms with good quality, high resistance and wide adaptability from Southern China to widen the GD between Huazhan derived lines and the male sterile subgroup developed by Hunan Longping High-tech Seed Science Research Institute Co., Ltd., so as to further promote the heterosis level between the groups.展开更多
In the present paper, to build model of two-line queuing system with losses GI/G/2/0, the approach introduced by V.S. Korolyuk and A.F. Turbin, is used. It is based on application of the theory of semi-Markov processe...In the present paper, to build model of two-line queuing system with losses GI/G/2/0, the approach introduced by V.S. Korolyuk and A.F. Turbin, is used. It is based on application of the theory of semi-Markov processes with arbitrary phase space of states. This approach allows us to omit some restrictions. The stationary characteristics of the system have been defined, assuming that the incoming flow of requests and their service times have distributions of general form. The particular cases of the system were considered. The used approach can be useful for modeling systems of various purposes.展开更多
The miscibility of flue gas and different types of light oils is investigated through slender-tube miscible displacement experiment at high temperature and high pressure.Under the conditions of high temperature and hi...The miscibility of flue gas and different types of light oils is investigated through slender-tube miscible displacement experiment at high temperature and high pressure.Under the conditions of high temperature and high pressure,the miscible displacement of flue gas and light oil is possible.At the same temperature,there is a linear relationship between oil displacement efficiency and pressure.At the same pressure,the oil displacement efficiency increases gently and then rapidly to more than 90% to achieve miscible displacement with the increase of temperature.The rapid increase of oil displacement efficiency is closely related to the process that the light components of oil transit in phase state due to distillation with the rise of temperature.Moreover,at the same pressure,the lighter the oil,the lower the minimum miscibility temperature between flue gas and oil,which allows easier miscibility and ultimately better performance of thermal miscible flooding by air injection.The miscibility between flue gas and light oil at high temperature and high pressure is more typically characterized by phase transition at high temperature in supercritical state,and it is different from the contact extraction miscibility of CO_(2) under conventional high pressure conditions.展开更多
Drought is one of the important factors limiting rice production,and the application of new water-saving rice varieties is urgently needed in rice production.Wanliangyou 1008 bred by Institute of Rice Research,Anhui A...Drought is one of the important factors limiting rice production,and the application of new water-saving rice varieties is urgently needed in rice production.Wanliangyou 1008 bred by Institute of Rice Research,Anhui Academy of Agricultural Sciences is a new two line hybrid rice variety with some good traits,including high drought resistance,high yield and good disease resistance.Wanlingyou 1008 was obtained by crossing drought-resistant high-quality restorer line DJ1008 and disease-resistant two-line sterile line Wan 25S.In this paper,the breeding process,characteristics and seed production technology of the variety were described in detail.展开更多
Xinliangyou 998 is a new indica two-line hybrid rice variety using Xin＇er Sx H0998 as parent. The variety was introduced and demonstrated in Yingshan County in 2016, and showed intermediate growth duration, strong ti...Xinliangyou 998 is a new indica two-line hybrid rice variety using Xin＇er Sx H0998 as parent. The variety was introduced and demonstrated in Yingshan County in 2016, and showed intermediate growth duration, strong tillering ability, firm culm, tolerance to high fertility and resistance to lodging, tolerance to high tempera- ture and wide range of adaptation, etc. The high-yield cultural techniques were con- cluded.展开更多
There are various issues for CO_(2)flooding and storage in Shengli Oilfield,which are characterized by low light hydrocarbon content of oil and high miscible pressure,strong reservoir heterogeneity and low sweep effic...There are various issues for CO_(2)flooding and storage in Shengli Oilfield,which are characterized by low light hydrocarbon content of oil and high miscible pressure,strong reservoir heterogeneity and low sweep efficiency,gas channeling and difficult whole-process control.Through laboratory experiments,technical research and field practice,the theory and technology of CO_(2)high pressure miscible flooding and storage are established.By increasing the formation pressure to 1.2 times the minimum miscible pressure,the miscibility of the medium-heavy components can be improved,the production percentage of oil in small pores can be increased,the displacing front developed evenly,and the swept volume expanded.Rapid high-pressure miscibility is realized through advanced pressure flooding and energy replenishment,and technologies of cascade water-alternating-gas(WAG),injection and production coupling and multistage chemical plugging are used for dynamic control of flow resistance,so as to obtain the optimum of oil recovery and CO_(2)storage factor.The research results have been applied to the Gao89-Fan142 in carbon capture,utilization and storage(CCUS)demonstration site,where the daily oil production of the block has increased from 254.6 t to 358.2 t,and the recovery degree is expected to increase by 11.6 percentage points in 15 years,providing theoretical and technical support for the large-scale development of CCUS.展开更多
The high temperature and high pressure visualization pressure-volume-temperature(PVT)experiments of different gas media-crude oil were carried using the interface disappearance method.There are two miscible temperatur...The high temperature and high pressure visualization pressure-volume-temperature(PVT)experiments of different gas media-crude oil were carried using the interface disappearance method.There are two miscible temperature domains in the miscibility of CO_(2)-crude oil during heating process under constant pressure.Under the experiment pressure of 15 MPa,when the temperature is less than 140℃,the miscible zone shows liquid phase characteristics,and increasing the temperature inhibits the miscible process;when the temperature is greater than 230℃,the miscible zone tends to show gas phase characteristics,and increasing the temperature is conducive to the miscibility formation.Under a certain pressure,with the increase of temperature,the miscibility of flue gas,nitrogen and crude oil is realized.When the temperature is low,the effect of CO_(2) on promoting miscibility is obvious,and the order of miscible temperature of gas medium and crude oil is N_(2)>flue gas>CO_(2);however,when the temperature is high,the effect of CO_(2) on promoting miscibility gradually decreases,and the miscible temperature of N_(2) and crude oil is close to that of flue gas.The miscibility is dominated by the distillation and volatilization of light components of crude oil.There are many light hydrocarbon components in the gas phase at phase equilibrium,and the miscible zone is characterized by gas phase.展开更多
基金Supported by the Project for the Commercialization of Agricultural Research Findings in China(2011GB2D200007)the National High Technology Research and Development Program of China (863 Program) Planning for the Development of High-Technology Research in China(2010AA101304)the National Natural Science Foundation of China(31371714)~~
文摘The insecure problems of seed production have seriously hampered the healthy and sustainable development of two-line hybrid rice.The safety issues on seed production of two-line hybrid rice and current research situation were pointed out in this paper.The three main reasons for unsafety in seed production of twoline hybrid rice were unsuitable site selection,high critical sterility-inducing temperature and the drift of critical temperature.In this paper,strategies and measures were put forward based on many years of practice.It could minimize the risk in seed production of two-line hybrid rice by selecting dual-purpose genic male sterile line with lower critical sterility-inducing temperature and long lower temperature resistant time.Based on the climate data and climatic demands of the ＂three safe-periods＂ in seed production,a new idea for determining appropriate bases and periods for seed production was proposed by using computer technology,which solved the aimless selection of sites and periods for the seed production of two-line hybrid rice.Besides,we established a system of single plant selection and identification method and original seed propagation with cyclic cold water,which could reduce the generation number of original seed propagation in seed production and avoid the drift of critical sterility-inducing temperature.This paper improved the seed production safety in the three aspects of seed nature,seed source and seed production site.
基金Supported by The Ministry of National Agricultural Special PublicSector Research "Nuclear Technology Application in Agriculture"(No.200803034)Science and Technology Innovation Projects ofHunan Academy of Agricultural Sciences(2009hnnkycx13)the National Science&Technology Pillar Program in the Eleventh Five-year Plan period(2008BAD7B02)~~
文摘Seeds of two-line sterile rice cultivars Zhu 1S and Lu 18S were carried into space by ＂Shijian 8＂ breeding recoverable satellite, then planted in ground. Mutagenic effects from space induction were compared with those from y-irradiation and complex processing of space induction and y-irradiation. The results showed that agronomic effect was stimulated in Mo progenies of the two-line sterile rice varieties treated by space flight, and their radiosensitivities to the irradiation of space flight performed non-sensitive. The order of mutation frequency was determined to be SP ＋ γ 〉 γ 〉 SP in M2 generation. And a series of mutated elites（individuals） were screened. Physiological indices of mutants screened like the activity of protective enzymes were measured to explore the physiological and biochemical basis of biological effect in space environment to two-line sterile rice. The results of this study show that space mutation breeding is an effective novel mean for breeding.
基金Supported by Special Scientific Research Fund of Agricultural Public Welfare Profession(200903008-09)~~
文摘[Objective] The aim was to research effects of N quantity on grain-filling characters of two-line hybrid rice cultivars with large ears. [Method] Peiza 67 and 88, two-line hybridized rice with large ears, were made use of to study on effects of N fertilizer in different quantities （LN： 90 kg/hm2;MN： 180 kg/hm2;HN： 270 kg/hm2） on plumpness and grain-filling characters. [Result] When N fertilizers were excessive, for inferior grains, grain-filling rate decreased and grain-filling time extended, resulting in plumpness decline after degradation of leaves＇ function. When N fertilizers were inadequate, maximal and average grain-filling rates decreased and the differences between superior and inferior grains in grain-filling rate increased, leading to decline of grain＇s weight and plumpness degree. On the other hand, quantity of N fertilizers had little effect on superior grains in plumpness. [Conclusion] The research provided references for reasonable use of N fertilizer and improvement of rice yield and N use.
基金Supported by the Project of "Six Talent Peaks"of Jiangsu Province(No.[2014]346 NY-040)"333 Talent Project"of Jiangsu Province(BRA2014142)~~
文摘The breeding processes, characteristics and comprehensive characters of two-line hybrid rice Yanliangyou 2208 and its parents （Yan 220S and Yanhui 888） taking part in the national rice regional test of southern rice regions were introduced. Yanliangyou 2208 was approved by the National Crop Variety Appraisal Committee in 2013. The achievement has been transformed successfully and has been applied in production. It has good development and promotion prospects.
基金Supported by Tianjin University of Science & Technology LaboratoryOpen Fund(1014A109)National 863 Science Plan(2010AA101301)National Natural Science Foundation(20903071)~~
文摘[Objective]The research aimed at constructing an accumulated temperature effect model for fertility alteration of two-line hybrid rice P88S.[Method]Analyze the experiment datas of fertility alteration of P88S in Hainan from 2005 to 2006.[Result]The fertilities of P88S with temperatures ranging from 26 to 28 ℃ were determined by the absolute values and the accumulation effects of the temperature.An accumulation effect model of temperatures based on the bistable state was obtained.[Conclusion]The temperature asymmetry of fertility alteration for two-line sterility line in spring and autumn can be elucidated effectively using the accumulated temperature effect model,namely,alternating from fertile to sterile requires higher temperatures,and alternating from sterile to fertile requires lower temperatures.
基金supported by the National High Technology Research and Development Program of (Grant No.2010AA101304)the Transformation Fund for Agricultural Science and Technology Achievements (Grant No.2007GB2D200226)the Natural Science Foundation of Hunan Province,China(Grant No. 10JJ4012)
文摘The main problems about research and application of two-line hybrid rice were reviewed, including the confusing nomenclature and male sterile lines classification, the unclear characteristics of photoperiod and temperature responses and the unsuitable site selection for male sterile line and hybrid dce seed production. In order to efficiently and accurately use dual-purpose genic male sterile lines, four types, including PTGMS （photo-thermo-sensitive genic male sterile rice）, TGMS （thermo-sensitive genic male sterile rice）, reverse PTGMS and reverse TGMS, were proposed. A new idea for explaining the mechanism of sterility in dual-purpose hybrid rice was proposed. The transition from sterile to fertile was involved in the cooperative regulation of major-effect sterile genes and photoperiod and/or temperature sensitive ones. The minor-effect genes with accumulative effect on sterility were important factors that affected the critical temperature of sterility transfer. In order to make better use of dual-purpose lines, the characterization of responses to photoperiod and temperature of PTGMS should be made and the identification method for the characterization of photoperiod and temperature responses of PTGMS should also be put forward. The optimal ecological site for seed production could be determined according to the historical climate data and the requirements for the meteorological conditions during the different periods of seed production.
基金The research was supported by the National Natural Science Foundation of China （30370830）.
文摘By inducing frequency, intensity and duration of lower temperature in the middle and last ten-day periods in August in the rice-growing areas of southern China, increasing temperature for safe seed production was defined as 2℃. During the sensitive period of fertility, characters of panicle height and canopy structure of TGMS rice, Pei＇ai64S, were measured. Results showed that temperature changes caused by irrigation in fields were below 40 cm of rice plant, and heating effect was significant at 20 cm when the temperature was increased by 3.1 ℃. For the present study, the following irrigation techniques were put forth： the water depth of 15-20 cm, current water used, irrigating after 17：00 and bailing at 10：00 in sunny or cloudy weather, irrigating on whole day, in shady or rainy weather, increasing inflows and outflows in large fields. In the present experiment, pollen fertility and self-fertilized seed setting rate accepted that the techniques were feasible and effective for against lower temperature and safeguarding seed production of two-line hybrid rice.
基金This study was supported by the National Natural Science Foundation of China （30671225）the Natural Science Foundation of Jiangsu Province, China （BK2006069, BK2007071）the National Key Technologies R＆D Program, China （2006BAD02A13-3-2）.
文摘This study was designed to elucidate the grain filling characteristics and the causes of poor grain plumpness of some two-line hybrid rice combinations and their hormonal mechanism. Six varieties, including three two-line hybrid rice combinations, that show differences in seed-setting and grain filling, were used. And the contents of starch, sucrose, zeatin （Z） ＋ zeatin riboside （ZR）, indole-3-acetic acid （IAA）, and abscisic acid （ABA）, the ethylene evolution rate, activities of sucrose synthase （SuSase） and starch synthase （StSase） in grains, the seed-setting and grain filling rate were investigated. The correlations amongst these were analyzed. The results showed that the poor grain filling of two-line hybrids was mainly attributed to the higher unfilled grain rate and the lower filling degree of inferior grains. During the early and mid grain filling periods, the sucrose content in inferior grains was greater than that in superior grains for the combinations with poor grain filling, indicating that the substrate concentration was not the principal factor for their slow grain filling and poor grain plumpness of the inferior grains of two-line hybrids. Z ＋ ZR, IAA, and ABA in superior grains were obviously greater than those in inferior grains at early grain filling stage. The maximum and mean contents of Z ＋ ZR, IAA, and ABA were positively very significantly correlated with the maximum and mean grain-filling rate, filling degree, and grain weight. The evolution rate of ethylene was greater in inferior grains than in superior grains and greater for the combinations with poor grain plumpness than those with good grain plumpness at the early or mid filling stages. The evolution rate of ethylene was negatively and significantly correlated with the grain filling rate, the grain filling degree, and the grain weight. Spraying ethephon （ethylene-releasing agent） at the early grain filling stage increased the evolution rate of ethylene, reduced the ABA content and activities of SuSase and StSase, and decreased the grain filling degree and the grain weight. The results were reversed when cobatous nitrate （an inhibitor of ethylene synthesis） was applied. The results suggested that the hormones and their balance play a role in the regulation of grain filling and enzymatic activities, and the poor grain filling is attributed to the low contents of Z ＋ ZR, IAA, and ABA, and the high evolution rate of ethylene in the inferior grains of some two-line hybrid rice combinations. The results suggested that hormones play important roles in the grain filling of some two-line hybrid rice combinations, and their filling degree can be improved by regulating the hormonal contents.
基金Supported by Major Science and Technology Project of Hunan Province(2013FJ1008)~~
文摘[Objective] The aim of this study was to explore the inheritance pattern of the resistance of two-line hybrid rice to false smut. [Method] A moderate susceptible TGMS line 33S as the female parent, was crossed with 30 susceptible restorer lines and 23 resistant restorer lines. Then false smut resistance of the hybrid F1 and parents were identified. [Result] All F1 of 33S crossed with 30 susceptible re- storer lines were susceptible to rice false smut. The F1 of 33S crossed with 23 re- sistant restorer lines showed dominant, incompletely dominant or recessive inheri- tance. Dominant and incompletely dominant inheritance accounted for 87.0%. The diseased panicle rate, disease index, average diseased grains per panicle, and the highest diseased grains per panicle of F1 shared highly significant positive correla- tions with corresponding indicators of restorer lines. [Conclusion] This study provides references for genetic improvement of two-line hybrid rice.
基金Supported by Open Project of State Key Laboratory of Hybrid Rice(2015KF05)China Postdoctoral Science Foundation(2015M572261)National Key Research and Development Program(2016YFD0101801)~~
文摘The genetic diversity of 50 parents or improved lines of two-line medium Indica hybrid rice were analyzed based on 48 pairs of SSR markers. The results showed that the clustering results were highly accordant with the parental pedigree information of the tested materials. The male sterile lines and the restoring lines were clustered into 2 distinct groups （Ⅰ and Ⅱ）, respectively. There were no significant difference in intra-group average genetic distances （GD） between the 2 groups. Most of the male sterile lines from Hunan Longping High-tech Seed Science Research Institute Co., Ltd. were grouped into a single subgroup （Ⅰ-4） under the male sterile group with the largest average GD compared with the rest male sterile subgroups. Huazhan derived lines and Guangzhou inbred lines were founded to be independent of 9311 and its derivatives or closely-related lines. Meanwhile, they have obviously been differentiated into 2 subgroups （Ⅱ-2 and Ⅱ-3）. The lines derived from Huazhan （or genetic closely related lines） showed a genetic homogenization phenomenon. Therefore, the Huazhan-derived lines should be further improved by the utilization of germplasms with good quality, high resistance and wide adaptability from Southern China to widen the GD between Huazhan derived lines and the male sterile subgroup developed by Hunan Longping High-tech Seed Science Research Institute Co., Ltd., so as to further promote the heterosis level between the groups.
文摘In the present paper, to build model of two-line queuing system with losses GI/G/2/0, the approach introduced by V.S. Korolyuk and A.F. Turbin, is used. It is based on application of the theory of semi-Markov processes with arbitrary phase space of states. This approach allows us to omit some restrictions. The stationary characteristics of the system have been defined, assuming that the incoming flow of requests and their service times have distributions of general form. The particular cases of the system were considered. The used approach can be useful for modeling systems of various purposes.
基金Supported by the PetroChina Science and Technology Project(2023ZG18).
文摘The miscibility of flue gas and different types of light oils is investigated through slender-tube miscible displacement experiment at high temperature and high pressure.Under the conditions of high temperature and high pressure,the miscible displacement of flue gas and light oil is possible.At the same temperature,there is a linear relationship between oil displacement efficiency and pressure.At the same pressure,the oil displacement efficiency increases gently and then rapidly to more than 90% to achieve miscible displacement with the increase of temperature.The rapid increase of oil displacement efficiency is closely related to the process that the light components of oil transit in phase state due to distillation with the rise of temperature.Moreover,at the same pressure,the lighter the oil,the lower the minimum miscibility temperature between flue gas and oil,which allows easier miscibility and ultimately better performance of thermal miscible flooding by air injection.The miscibility between flue gas and light oil at high temperature and high pressure is more typically characterized by phase transition at high temperature in supercritical state,and it is different from the contact extraction miscibility of CO_(2) under conventional high pressure conditions.
基金Supported by Anhui Provincial Science and Technology Major Project(202003a06020005,2021d06050002)Joint Research on Crop Varieties in Anhui Province(Rice)+1 种基金Innovation Team of Anhui Academy of Agricultural Sciences(2021YL004)Young Talents of Anhui Academy of Agricultural Sciences.
文摘Drought is one of the important factors limiting rice production,and the application of new water-saving rice varieties is urgently needed in rice production.Wanliangyou 1008 bred by Institute of Rice Research,Anhui Academy of Agricultural Sciences is a new two line hybrid rice variety with some good traits,including high drought resistance,high yield and good disease resistance.Wanlingyou 1008 was obtained by crossing drought-resistant high-quality restorer line DJ1008 and disease-resistant two-line sterile line Wan 25S.In this paper,the breeding process,characteristics and seed production technology of the variety were described in detail.
文摘Xinliangyou 998 is a new indica two-line hybrid rice variety using Xin＇er Sx H0998 as parent. The variety was introduced and demonstrated in Yingshan County in 2016, and showed intermediate growth duration, strong tillering ability, firm culm, tolerance to high fertility and resistance to lodging, tolerance to high tempera- ture and wide range of adaptation, etc. The high-yield cultural techniques were con- cluded.
基金Supported by the Sinopec"Ten Dragon"Major ProjectKey Research Projects of Sinopec(P22180)。
文摘There are various issues for CO_(2)flooding and storage in Shengli Oilfield,which are characterized by low light hydrocarbon content of oil and high miscible pressure,strong reservoir heterogeneity and low sweep efficiency,gas channeling and difficult whole-process control.Through laboratory experiments,technical research and field practice,the theory and technology of CO_(2)high pressure miscible flooding and storage are established.By increasing the formation pressure to 1.2 times the minimum miscible pressure,the miscibility of the medium-heavy components can be improved,the production percentage of oil in small pores can be increased,the displacing front developed evenly,and the swept volume expanded.Rapid high-pressure miscibility is realized through advanced pressure flooding and energy replenishment,and technologies of cascade water-alternating-gas(WAG),injection and production coupling and multistage chemical plugging are used for dynamic control of flow resistance,so as to obtain the optimum of oil recovery and CO_(2)storage factor.The research results have been applied to the Gao89-Fan142 in carbon capture,utilization and storage(CCUS)demonstration site,where the daily oil production of the block has increased from 254.6 t to 358.2 t,and the recovery degree is expected to increase by 11.6 percentage points in 15 years,providing theoretical and technical support for the large-scale development of CCUS.
基金Supported by the Petro China Science and Technology Project(2023ZG18)。
文摘The high temperature and high pressure visualization pressure-volume-temperature(PVT)experiments of different gas media-crude oil were carried using the interface disappearance method.There are two miscible temperature domains in the miscibility of CO_(2)-crude oil during heating process under constant pressure.Under the experiment pressure of 15 MPa,when the temperature is less than 140℃,the miscible zone shows liquid phase characteristics,and increasing the temperature inhibits the miscible process;when the temperature is greater than 230℃,the miscible zone tends to show gas phase characteristics,and increasing the temperature is conducive to the miscibility formation.Under a certain pressure,with the increase of temperature,the miscibility of flue gas,nitrogen and crude oil is realized.When the temperature is low,the effect of CO_(2) on promoting miscibility is obvious,and the order of miscible temperature of gas medium and crude oil is N_(2)>flue gas>CO_(2);however,when the temperature is high,the effect of CO_(2) on promoting miscibility gradually decreases,and the miscible temperature of N_(2) and crude oil is close to that of flue gas.The miscibility is dominated by the distillation and volatilization of light components of crude oil.There are many light hydrocarbon components in the gas phase at phase equilibrium,and the miscible zone is characterized by gas phase.
