Circularly polarized luminescence(CPL)and two-photon absorption(TPA)materials have garnered considerable attentions due to their minimal energy loss and superior optical penetration[1,2].However,the current challenge ...Circularly polarized luminescence(CPL)and two-photon absorption(TPA)materials have garnered considerable attentions due to their minimal energy loss and superior optical penetration[1,2].However,the current challenge lies in the absence of well-developed strategies for designing materials that combine these two exceptional optical properties.展开更多
The title compound, trans 4 [p (N ethyl N (hydroxyethyl)amino) styryl] N methylpyridinium tetraphenylborate(abbreviated as ESMT, C 46 H 49 N 4OB) crystallizes in a space group P 1 with ...The title compound, trans 4 [p (N ethyl N (hydroxyethyl)amino) styryl] N methylpyridinium tetraphenylborate(abbreviated as ESMT, C 46 H 49 N 4OB) crystallizes in a space group P 1 with a =0 983 23(15) nm, b =1 410 73(16) nm, c =1 54 21(12) nm, α=95 252(13)°, β= 91 959(17)°, γ= 107 421(12)°, Z=2, F(000)=712, μ =0 1 mm -1 Mo Kα radiation, R =0 093 8. The result shows that the cation of the molecule almost lies in one plane, the hydroxyethyl group is perpendicular to the plane, the anion takes a slight distorted tetrahedral geometry. Also the thermal stability, two photon fluorescence, two photon pumped lasing property for the title compound were studied.展开更多
The present work concerns the study of solvent effects on the geometrical structures, as well as one- and two-photon absorption （TPA） processes, for two series of alkyne and alkene π-bridging molecules, within the ...The present work concerns the study of solvent effects on the geometrical structures, as well as one- and two-photon absorption （TPA） processes, for two series of alkyne and alkene π-bridging molecules, within the framework of the polarization continuum model. Particular emphasis was put on the characterization of solvent effects on the molecular geometrical structures and geometric distortion, which were measured by the bond-length-alternation parameter. The π centres in the compounds are seen to play a decisive role in increasing the TPA cross section and nonlinear optical properties. All studied molecules have relatively strong TPA characteristics, while the alkyne π-bridging ones yield larger TPA cross sections.展开更多
Near-infrared(NIR) nanoparticles(NPs) based on fluorescence resonance energy transfer(FRET) were prepared by coencapsulation of a red aggregation-induced emission(AIE) molecule, 2-(4-bromophenyl)-3-(4-(4-(diphenylamin...Near-infrared(NIR) nanoparticles(NPs) based on fluorescence resonance energy transfer(FRET) were prepared by coencapsulation of a red aggregation-induced emission(AIE) molecule, 2-(4-bromophenyl)-3-(4-(4-(diphenylamino)styryl)phenyl)fumaronitrile(TB), and a commercial NIR fluorescence dye, silicon 2,3-naphthalocyanine bis(trihexylsilyloxide)(NIR775) with an amphiphilic polymer poly(styrene-co-maleic anhydride)(PSMA). The surface of the NPs, PSMA@TB/NIR775, was modified with poly(ethylene glycol)(PEG) to increase the in vivo biocompatibility of the NPs. The PSMA@TB/NIR775 NPs showed a strong NIR(780 nm) narrow emission and excellent two-photon absorption property. Moreover, the NPs exhibited good monodispersity, stability, and low cytotoxicity.Under the excitation of a 1040 nm femtosecond(fs) laser, the emission peaks at 680 nm of TB and 780 nm of NIR775 excited by FRET were obtained. We utilized PSMA@TB/NIR775 NPs as fluorescent contrast agents for two-photon excited NIR microscopic imaging, and good NIR imaging effect of mouse brain vasculature was obtained with the imaging depth of about 150 μm. The FRET strategy by coencapsulating AIE molecule and NIR dye will be helpful in preparing more narrow emission NIR probes for deep-tissue biological imaging.展开更多
PAHPN. a naphthalimide-based mitotracker with reasonable two-photon excitation emission activity and polarity-sensitive fluorescence properties has been efficiently synthesized and studied in twophoton, co-localizatio...PAHPN. a naphthalimide-based mitotracker with reasonable two-photon excitation emission activity and polarity-sensitive fluorescence properties has been efficiently synthesized and studied in twophoton, co-localization, and FLIM imaging.展开更多
In this paper,the energy spectrum of the two-photon Jaynes-Cummings model（TPJCM） is calculated exactly in the non-rotating wave approximation（non-RWA）,and we study the level-crossing problem by means of fidelity.A...In this paper,the energy spectrum of the two-photon Jaynes-Cummings model（TPJCM） is calculated exactly in the non-rotating wave approximation（non-RWA）,and we study the level-crossing problem by means of fidelity.A narrow peak of the fidelity is observed at the level-crossing point,which does not appear at the avoided-crossing point.Therefore fidelity is perfectly suited for detecting the level-crossing point in the energy spectrum.展开更多
Two new bithiophene derivatives named as 5, 5-bis(p-N,N-dimethylaminostyryl)-2, 2 -bithiophene (BMSBT), and 5, 5-bis(p-N,N-diethylaminostyryl)-2, 2-bithiophene (BESBT) have been synthesized. Both compounds can emit s...Two new bithiophene derivatives named as 5, 5-bis(p-N,N-dimethylaminostyryl)-2, 2 -bithiophene (BMSBT), and 5, 5-bis(p-N,N-diethylaminostyryl)-2, 2-bithiophene (BESBT) have been synthesized. Both compounds can emit strong single-photon excited fluorescence (SPEF) and two-photon excited fluorescence (TPEF) with the emission peaks around ~560 nm and with the lifetime of ~1ns.展开更多
Mitochondria and lysosomes are essential cellular organelles in most eukaryotic cells by playing the physiological roles to support the normal functions of cells, as well as the life of the whole body. To date,small-m...Mitochondria and lysosomes are essential cellular organelles in most eukaryotic cells by playing the physiological roles to support the normal functions of cells, as well as the life of the whole body. To date,small-molecule fluorescent probes have been considered as one of the vital tools for monitoring and visualizing multiple biological analytes. This review summarized the recent advances in small-molecule two-photon fluorescent probes for metal ions, reactive oxygen species（ROS） and reactive sulfur species（RSS）, and changes inside micro-environment（e.g., p H, viscosity and polarity） in mitochondria and lysosomes, or served as mitotracker and lysotracker with the assistance of two-photon microscopy.展开更多
Arbitrary micro-scale three-dimensional(3D)structures fabrication is a dream to achieve many exciting goals that have been pursued for a long time.Among all these applications,the direct 3D printing to fabricate human...Arbitrary micro-scale three-dimensional(3D)structures fabrication is a dream to achieve many exciting goals that have been pursued for a long time.Among all these applications,the direct 3D printing to fabricate human organs and integrated photonic circuits are extraordinary attractive as they can promote the current technology to a new level.Among all the 3D printing methods available,two-photon polymerization(2PP)is very competitive as it is the unique method to achieve sub-micron resolution to make any desired tiny structures.For the conventional 2PP,the building block is the photoresist.However,the requirement for the building block is different for different purposes.It is very necessary to investigate and improve the photoresist properties according to different requirements.In this paper,we presented one hybrid method to modify the mechanical strength and light trapping efficiency of the photoresist,which transfers the photoresist into the micro-concretes.The micro-concrete structure can achieve±22%strength modification via a silica nano-particles doping.The structures doped with gold nano-particles show tunable plasmonic absorption.Dye doped hybrid structure shows great potential to fabricate 3D micro-chip laser.展开更多
Efficient Ti-catalyzed reductive coupling methodology was first employed to synthesize the symmetrical bis-donor stilbene, trans-4, 4'-bis[diphenyl amino] stilbene (BDPAS). X-ray diffraction analyses reveal that t...Efficient Ti-catalyzed reductive coupling methodology was first employed to synthesize the symmetrical bis-donor stilbene, trans-4, 4'-bis[diphenyl amino] stilbene (BDPAS). X-ray diffraction analyses reveal that this new crystal belongs to the triclinic crystal system of centro-symmetric P-1 space group. The DBPAS solution, with the linear transmission at wavelength of greater than or equal to 450 nm, possesses large two-photon absorption cross section as high as 39.4x10(-48) cm(4).s/photon resulting in strong two-photon induced blue fluorescence of 460 nm, pumped by 740 nm laser irradiation.展开更多
Enzymes are macromolecular biological catalysts which can accelerate chemical reactions in living organisms. Almost all the physiological metabolism activities in the cell need enzymes to sustain life via rapid cataly...Enzymes are macromolecular biological catalysts which can accelerate chemical reactions in living organisms. Almost all the physiological metabolism activities in the cell need enzymes to sustain life via rapid catalysis. Currently, medical research has proved that abnormal enzyme activity is associated with numerous diseases, such as Parkinson’s disease(PD), Alzheimer’s disease(AD) and cancers. On the other hand, early diagnosis of those diseases is of great significance to improve the survival rate and cure rate.In the current diagnostic tools, two-photon fluorescent probes(TPFPs) are developing rapidly due to their unique advantages, such as higher spatial resolution, deeper imaging depth, and lower biotoxicity.Therefore, the design and synthesis of two-photon(TP) small molecule enzymatic probes have broad prospects for early diagnosis and treatment of diseases. As of now, scientists have developed many TP small molecule enzymatic probes. This review aims to summarize the TP small molecule enzymatic probes and expound the reaction mechanism.展开更多
An electron donor-π-bridge-electron acceptor(D-π-A) optical functional organic compound comprising a triphenylamine moiety as the electron donor and pyridine moiety as the electron acceptor was synthesized. The stru...An electron donor-π-bridge-electron acceptor(D-π-A) optical functional organic compound comprising a triphenylamine moiety as the electron donor and pyridine moiety as the electron acceptor was synthesized. The structure of the compound was solved by single-crystal X-ray diffraction analysis. It crystallizes in monoclinic, space group P21, with a = 9.753(5), b = 8.815(5), c = 25.554(5) ?, β = 96.315(5)°, V = 2184(2) ?~3, Z = 2, D_c = 1.136 g/m^3, F(000) = 792, Μr = 746.92, μ = 0.069 mm^(-1), the final R = 0.0658 and wR = 0.1730 for 6790 observed reflections with I > 2(I). Study of nonlinear optical properties shows that the compound exhibits excellent two-photon excited fluorescence with the two-photon absorption cross-section value of 116 GM. The structure-property relationship was researched in detail through X-ray crystallography and quantum chemical calculation. Result of living cell imaging experiment shows its potential in fluorescence microscopy bioimaging.展开更多
A novel donor-acceptor(D-A) type of two-photon(TP) fluorescent probe,i.e.Lyso-OSC,based on the lysosome-targeting morpholine group was developed.The polarity sensing coumarin group was functionalized as the acceptor a...A novel donor-acceptor(D-A) type of two-photon(TP) fluorescent probe,i.e.Lyso-OSC,based on the lysosome-targeting morpholine group was developed.The polarity sensing coumarin group was functionalized as the acceptor and the 1-vinyl-4-methoxybenzene group was engineered as the donor.The fluorescence intensity and emission maximum wavelength of Lyso-OSC are highly sensitive to the polarity changes of solvent.The two-photon absorption cross-section and tissue penetration depth are up to 254 GM and 150 μm,respectively.The strong fluorescence,high sensitivity to polarity,low cytotoxicity,and accurate lysosome-targeting ability entail Lyso-OSC the excellent performance in detecting the polarity changes ofcellular environment.To this end,a bright,real-time imaging autophagy of living cells has been achieved.展开更多
Considering two atomic qubits initially in Bell states, we send one qubit into a vacuum cavity with two-photon resonance and leave the other one outside. Using quantum information entropy squeezing theory, the time ev...Considering two atomic qubits initially in Bell states, we send one qubit into a vacuum cavity with two-photon resonance and leave the other one outside. Using quantum information entropy squeezing theory, the time evolutions of the entropy squeezing factor of the atomic qubit inside the cavity are discussed for two cases, i.e., before and after rotation and measurement of the atomic qubit outside the cavity. It is shown that the atomic qubit inside the cavity has no entropy squeezing phenomenon and is always in a decoherent state before the operating atomic qubit outside the cavity. However,the periodical entropy squeezing phenomenon emerges and the optimal entropy squeezing state can be prepared for the atomic qubit inside the cavity by adjusting the rotation angle, choosing the interaction time between the atomic qubit and the cavity, controlling the probability amplitudes of subsystem states. Its physical essence is cutting the entanglement between the atomic qubit and its environment, causing the atomic qubit inside the cavity to change from the initial decoherent state into maximum coherent superposition state, which is a possible way of recovering the coherence of a single atomic qubit in the noise environment.展开更多
We theoretically study the system of a superconducting transmission line resonator coupled to two interacting super- conducting flux qubits. It is shown that under certain conditions the resonator mode can be tuned to...We theoretically study the system of a superconducting transmission line resonator coupled to two interacting super- conducting flux qubits. It is shown that under certain conditions the resonator mode can be tuned to two-photon resonance between the ground state and the highest excited state while the middle excited states are far-off resonance. Furthermore, we study the steady-state properties of the flux qubits and resonator, such as the photon statistics, the spectrum and squeezing of the resonator, and demonstrate that two-photon laser can be implemented with current experimental technology.展开更多
A phase-stabilized femtosecond frequency comb is used to measure high-resolution spectra of two-photon transition 62S1/2-62P1/2,3/2-82S1/2 in a cesium vapor. The broadband laser output from a femtosecond frequency com...A phase-stabilized femtosecond frequency comb is used to measure high-resolution spectra of two-photon transition 62S1/2-62P1/2,3/2-82S1/2 in a cesium vapor. The broadband laser output from a femtosecond frequency comb is split into counter-propagating parts, shaped in an original way, and focused into a room-temperature cesium vapor. We obtain high-resolution two-photon spectroscopy by scanning the repetition rate of femtosecond frequency comb, and through absolute frequency measurements.展开更多
A two-photon fluorescent probe TPZn was developed for specific ratiometric imaging Zn2＋ in living cells and tissues. Significant ratiometric fluorescence change was based on photoinduced electron transfer and intramo...A two-photon fluorescent probe TPZn was developed for specific ratiometric imaging Zn2＋ in living cells and tissues. Significant ratiometric fluorescence change was based on photoinduced electron transfer and intramolecular charge transfer. The synthetic method of TPZn was simple. It was successfully used to selectively image Zn2＋ based on the higher binding affinity for Zn2＋ than for Cd2＋. TPZn was easily loaded into the living cell and tissues with high membrane permeability in a complex biological environment. TPZn could clearly visualize endogenous Zn2＋ by TP ratiometric imaging in hippocampal slices at a depth of 120 μm. Thus, TPZn is a useful tool to image of Zn2＋ in living cells and tissues without interference from Cd2＋.展开更多
A new two-photon fluorescent probe, ADNO, for nitric oxide （NO） based on intramolecular photoinduced electron transfer （PET） mechanism d/splays a rapid response to NO with a remarkable fluorescent enhancement in P...A new two-photon fluorescent probe, ADNO, for nitric oxide （NO） based on intramolecular photoinduced electron transfer （PET） mechanism d/splays a rapid response to NO with a remarkable fluorescent enhancement in PBS buffer. The excellent chemoselectivity of ADNO for NO over other ROS/RNS （reactive oxygen species or nitrogen species） and common metal ions was observed. Moreover, ADNO has been successfully applied in fluorescence imaging of NO of living cells using both one-photon microscopy （OPM） and two-~hoton microscopy （TPM）,展开更多
The local density of photonic states （LDPS） of an infinite two-dimensional （2D） photonic crystal （PC） composed of rotated square-pillars in a 2D square lattice is calculated in terms of the plane-wave expansion ...The local density of photonic states （LDPS） of an infinite two-dimensional （2D） photonic crystal （PC） composed of rotated square-pillars in a 2D square lattice is calculated in terms of the plane-wave expansion method in a combination with the point group theory. The calculation results show that the LDPS strongly depends on the spatial positions. The variations of the LDPS as functions of the radial coordinate and frequency exhibit “mountain chain” structures with sharp peaks. The LDPS with large value spans a finite area and falls abruptly down to small value at the position corresponding to the interfaces between two different refractive index materials. The larger/lower LDPS occurs inward the lower/larger dielectric-constant medium. This feature can be well interpreted by the continuity of electricdisplacement vector at the interface. In the frequency range of the pseudo-PBG （photonic band gap）, the LDPS keeps very low value over the whole Wiger-Seitz cell. It indicates that the spontaneous emission in 2D PCs cannot be prohibited completely, but it can be inhibited intensively when the resonate frequency falls into the pseudo-PBG.展开更多
Two novel V-shaped symmetric chromophores： E-2,8-bis（4-vinyl-4-carbazol-9-yl）diben- zothiophene （abbreviated as SK-G1） and E-2,8-bis（4-vinyl-4-triphenylamino） dibenzothiophene （abbreviated as ST-G1） have been...Two novel V-shaped symmetric chromophores： E-2,8-bis（4-vinyl-4-carbazol-9-yl）diben- zothiophene （abbreviated as SK-G1） and E-2,8-bis（4-vinyl-4-triphenylamino） dibenzothiophene （abbreviated as ST-G1） have been synthesized and characterized. Their two photon absorption properties were measured by the open-aperture femtosecond Z-scan technique and the nanosecond nonlinear optical transmission （NLT）, respectively, when pumped by Ti： sapphire laser at 750 nm and 800 nm.展开更多
基金supported by NSFC(22271282)the Self-deployment Project Research Program of Haixi Institutes,Chinese Academy of Sciences with the grant number of CXZX-2022-JQ04.
文摘Circularly polarized luminescence(CPL)and two-photon absorption(TPA)materials have garnered considerable attentions due to their minimal energy loss and superior optical penetration[1,2].However,the current challenge lies in the absence of well-developed strategies for designing materials that combine these two exceptional optical properties.
文摘The title compound, trans 4 [p (N ethyl N (hydroxyethyl)amino) styryl] N methylpyridinium tetraphenylborate(abbreviated as ESMT, C 46 H 49 N 4OB) crystallizes in a space group P 1 with a =0 983 23(15) nm, b =1 410 73(16) nm, c =1 54 21(12) nm, α=95 252(13)°, β= 91 959(17)°, γ= 107 421(12)°, Z=2, F(000)=712, μ =0 1 mm -1 Mo Kα radiation, R =0 093 8. The result shows that the cation of the molecule almost lies in one plane, the hydroxyethyl group is perpendicular to the plane, the anion takes a slight distorted tetrahedral geometry. Also the thermal stability, two photon fluorescence, two photon pumped lasing property for the title compound were studied.
文摘The present work concerns the study of solvent effects on the geometrical structures, as well as one- and two-photon absorption （TPA） processes, for two series of alkyne and alkene π-bridging molecules, within the framework of the polarization continuum model. Particular emphasis was put on the characterization of solvent effects on the molecular geometrical structures and geometric distortion, which were measured by the bond-length-alternation parameter. The π centres in the compounds are seen to play a decisive role in increasing the TPA cross section and nonlinear optical properties. All studied molecules have relatively strong TPA characteristics, while the alkyne π-bridging ones yield larger TPA cross sections.
基金financially supported by the National Natural Science Foundation of China (Nos. 21835001, 51773080, 21674041, 51573068, and 21221063)Program for Changbaishan Scholars of Jilin Province, Jilin Province (No. 20160101305JC)the "Talents Cultivation Program" of Jilin University
文摘Near-infrared(NIR) nanoparticles(NPs) based on fluorescence resonance energy transfer(FRET) were prepared by coencapsulation of a red aggregation-induced emission(AIE) molecule, 2-(4-bromophenyl)-3-(4-(4-(diphenylamino)styryl)phenyl)fumaronitrile(TB), and a commercial NIR fluorescence dye, silicon 2,3-naphthalocyanine bis(trihexylsilyloxide)(NIR775) with an amphiphilic polymer poly(styrene-co-maleic anhydride)(PSMA). The surface of the NPs, PSMA@TB/NIR775, was modified with poly(ethylene glycol)(PEG) to increase the in vivo biocompatibility of the NPs. The PSMA@TB/NIR775 NPs showed a strong NIR(780 nm) narrow emission and excellent two-photon absorption property. Moreover, the NPs exhibited good monodispersity, stability, and low cytotoxicity.Under the excitation of a 1040 nm femtosecond(fs) laser, the emission peaks at 680 nm of TB and 780 nm of NIR775 excited by FRET were obtained. We utilized PSMA@TB/NIR775 NPs as fluorescent contrast agents for two-photon excited NIR microscopic imaging, and good NIR imaging effect of mouse brain vasculature was obtained with the imaging depth of about 150 μm. The FRET strategy by coencapsulating AIE molecule and NIR dye will be helpful in preparing more narrow emission NIR probes for deep-tissue biological imaging.
基金National Natural Science Foundation of China(Nos.21174022,21376038)National Basic Research Program of China(No.2013CB733702)+1 种基金Key Project of the Education Department of Sichuan Province(No.12ZA087)Specialized Research Fund for the Doctoral Program of Higher Education(No.20110041110009)
文摘PAHPN. a naphthalimide-based mitotracker with reasonable two-photon excitation emission activity and polarity-sensitive fluorescence properties has been efficiently synthesized and studied in twophoton, co-localization, and FLIM imaging.
基金Project supported by the National Natural Science Foundation of China (Grant No. 1097602/A06)
文摘In this paper,the energy spectrum of the two-photon Jaynes-Cummings model（TPJCM） is calculated exactly in the non-rotating wave approximation（non-RWA）,and we study the level-crossing problem by means of fidelity.A narrow peak of the fidelity is observed at the level-crossing point,which does not appear at the avoided-crossing point.Therefore fidelity is perfectly suited for detecting the level-crossing point in the energy spectrum.
基金This work is supported by the National Natural Science Foundation of China(No.20172034)grant for State Key Program of China.
文摘Two new bithiophene derivatives named as 5, 5-bis(p-N,N-dimethylaminostyryl)-2, 2 -bithiophene (BMSBT), and 5, 5-bis(p-N,N-diethylaminostyryl)-2, 2-bithiophene (BESBT) have been synthesized. Both compounds can emit strong single-photon excited fluorescence (SPEF) and two-photon excited fluorescence (TPEF) with the emission peaks around ~560 nm and with the lifetime of ~1ns.
基金supported by the National Natural Science Foundation of China (Nos. 21778001, 21372005)the Anhui Provincial Natural Science Foundation (No. 1608085MB39)+1 种基金the Natural Science Foundation of Education Department of Anhui Province (No. KJ2015A047)the 211 Project of Anhui University
文摘Mitochondria and lysosomes are essential cellular organelles in most eukaryotic cells by playing the physiological roles to support the normal functions of cells, as well as the life of the whole body. To date,small-molecule fluorescent probes have been considered as one of the vital tools for monitoring and visualizing multiple biological analytes. This review summarized the recent advances in small-molecule two-photon fluorescent probes for metal ions, reactive oxygen species（ROS） and reactive sulfur species（RSS）, and changes inside micro-environment（e.g., p H, viscosity and polarity） in mitochondria and lysosomes, or served as mitotracker and lysotracker with the assistance of two-photon microscopy.
基金financially supported by A*STAR, SERC 2014 Public Sector Research Funding (PSF) (Grant: SERC Project, 1421200080)973 Program of China (2013CBA01700)Chinese Nature Science Grant (61675207, U1609209)
文摘Arbitrary micro-scale three-dimensional(3D)structures fabrication is a dream to achieve many exciting goals that have been pursued for a long time.Among all these applications,the direct 3D printing to fabricate human organs and integrated photonic circuits are extraordinary attractive as they can promote the current technology to a new level.Among all the 3D printing methods available,two-photon polymerization(2PP)is very competitive as it is the unique method to achieve sub-micron resolution to make any desired tiny structures.For the conventional 2PP,the building block is the photoresist.However,the requirement for the building block is different for different purposes.It is very necessary to investigate and improve the photoresist properties according to different requirements.In this paper,we presented one hybrid method to modify the mechanical strength and light trapping efficiency of the photoresist,which transfers the photoresist into the micro-concretes.The micro-concrete structure can achieve±22%strength modification via a silica nano-particles doping.The structures doped with gold nano-particles show tunable plasmonic absorption.Dye doped hybrid structure shows great potential to fabricate 3D micro-chip laser.
文摘Efficient Ti-catalyzed reductive coupling methodology was first employed to synthesize the symmetrical bis-donor stilbene, trans-4, 4'-bis[diphenyl amino] stilbene (BDPAS). X-ray diffraction analyses reveal that this new crystal belongs to the triclinic crystal system of centro-symmetric P-1 space group. The DBPAS solution, with the linear transmission at wavelength of greater than or equal to 450 nm, possesses large two-photon absorption cross section as high as 39.4x10(-48) cm(4).s/photon resulting in strong two-photon induced blue fluorescence of 460 nm, pumped by 740 nm laser irradiation.
基金financially supported by the National Natural Science Foundation of China (No. 81672508)Jiangsu Provincial Foundation for Distinguished Young Scholars (No. BK20170041)+2 种基金Natural Science Foundation of Shaanxi Province (No. 2019JM-016)China-Sweden Joint Mobility Project (No. 51811530018)Fundamental Research Funds for the Central Universities
文摘Enzymes are macromolecular biological catalysts which can accelerate chemical reactions in living organisms. Almost all the physiological metabolism activities in the cell need enzymes to sustain life via rapid catalysis. Currently, medical research has proved that abnormal enzyme activity is associated with numerous diseases, such as Parkinson’s disease(PD), Alzheimer’s disease(AD) and cancers. On the other hand, early diagnosis of those diseases is of great significance to improve the survival rate and cure rate.In the current diagnostic tools, two-photon fluorescent probes(TPFPs) are developing rapidly due to their unique advantages, such as higher spatial resolution, deeper imaging depth, and lower biotoxicity.Therefore, the design and synthesis of two-photon(TP) small molecule enzymatic probes have broad prospects for early diagnosis and treatment of diseases. As of now, scientists have developed many TP small molecule enzymatic probes. This review aims to summarize the TP small molecule enzymatic probes and expound the reaction mechanism.
基金supported by the Major Project of Natural Science Research in Universities of Anhui Province(KJ2018ZD037,KJ2018A0333)Key Project of Youth Talents in Universities of Anhui Province(gxyqZD2017067)+5 种基金National Natural Science Foundation of China(21401024)Natural Science Foundation of Anhui Province(1508085MB21)National Students Research Training Program(201810371028)Research Innovation Team of Fuyang Normal University(kytd201710)Horizontal Cooperation Project of Fuyang Municipal Government and Fuyang Normal University(XDHX2016011,XDHX2016004)Anhui University Research Innovation Platform Team Project(201549)
文摘An electron donor-π-bridge-electron acceptor(D-π-A) optical functional organic compound comprising a triphenylamine moiety as the electron donor and pyridine moiety as the electron acceptor was synthesized. The structure of the compound was solved by single-crystal X-ray diffraction analysis. It crystallizes in monoclinic, space group P21, with a = 9.753(5), b = 8.815(5), c = 25.554(5) ?, β = 96.315(5)°, V = 2184(2) ?~3, Z = 2, D_c = 1.136 g/m^3, F(000) = 792, Μr = 746.92, μ = 0.069 mm^(-1), the final R = 0.0658 and wR = 0.1730 for 6790 observed reflections with I > 2(I). Study of nonlinear optical properties shows that the compound exhibits excellent two-photon excited fluorescence with the two-photon absorption cross-section value of 116 GM. The structure-property relationship was researched in detail through X-ray crystallography and quantum chemical calculation. Result of living cell imaging experiment shows its potential in fluorescence microscopy bioimaging.
基金supported by the National Natural Science Foundation of China(Nos.21778001,21672001)Open Fund for Discipline Construction in Institute of Physical Science and Information Technology of Anhui University。
文摘A novel donor-acceptor(D-A) type of two-photon(TP) fluorescent probe,i.e.Lyso-OSC,based on the lysosome-targeting morpholine group was developed.The polarity sensing coumarin group was functionalized as the acceptor and the 1-vinyl-4-methoxybenzene group was engineered as the donor.The fluorescence intensity and emission maximum wavelength of Lyso-OSC are highly sensitive to the polarity changes of solvent.The two-photon absorption cross-section and tissue penetration depth are up to 254 GM and 150 μm,respectively.The strong fluorescence,high sensitivity to polarity,low cytotoxicity,and accurate lysosome-targeting ability entail Lyso-OSC the excellent performance in detecting the polarity changes ofcellular environment.To this end,a bright,real-time imaging autophagy of living cells has been achieved.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11374096 and 11405052)
文摘Considering two atomic qubits initially in Bell states, we send one qubit into a vacuum cavity with two-photon resonance and leave the other one outside. Using quantum information entropy squeezing theory, the time evolutions of the entropy squeezing factor of the atomic qubit inside the cavity are discussed for two cases, i.e., before and after rotation and measurement of the atomic qubit outside the cavity. It is shown that the atomic qubit inside the cavity has no entropy squeezing phenomenon and is always in a decoherent state before the operating atomic qubit outside the cavity. However,the periodical entropy squeezing phenomenon emerges and the optimal entropy squeezing state can be prepared for the atomic qubit inside the cavity by adjusting the rotation angle, choosing the interaction time between the atomic qubit and the cavity, controlling the probability amplitudes of subsystem states. Its physical essence is cutting the entanglement between the atomic qubit and its environment, causing the atomic qubit inside the cavity to change from the initial decoherent state into maximum coherent superposition state, which is a possible way of recovering the coherence of a single atomic qubit in the noise environment.
基金Project supported by the National Fundamental Research Program of China(Grant No.2011cba00200)the National Natural Science Foundation of China(Grant No.11274295)the Doctor Foundation of Education Ministry of China(Grant No.20113402110059)
文摘We theoretically study the system of a superconducting transmission line resonator coupled to two interacting super- conducting flux qubits. It is shown that under certain conditions the resonator mode can be tuned to two-photon resonance between the ground state and the highest excited state while the middle excited states are far-off resonance. Furthermore, we study the steady-state properties of the flux qubits and resonator, such as the photon statistics, the spectrum and squeezing of the resonator, and demonstrate that two-photon laser can be implemented with current experimental technology.
基金Project supported by the National Basic Research Program of China (Grant No. 2012CB921603)the International Science & Technology Cooperation Program of China (Grant No. 2011DFA12490)+3 种基金the International Science & Technology Cooperation Program of Shanxi Province,China (Grant No. 2011081030)the National Natural Science Foundation of China (Grant Nos.61008012,10934004,60978001,11174187,and 11074154)the NSFC Project for Excellent Research Team of China (Grant No.61121064)the Specialized Research Fund for the Doctoral Program of Higher Education,China (Grant No. 20101401120004)
文摘A phase-stabilized femtosecond frequency comb is used to measure high-resolution spectra of two-photon transition 62S1/2-62P1/2,3/2-82S1/2 in a cesium vapor. The broadband laser output from a femtosecond frequency comb is split into counter-propagating parts, shaped in an original way, and focused into a room-temperature cesium vapor. We obtain high-resolution two-photon spectroscopy by scanning the repetition rate of femtosecond frequency comb, and through absolute frequency measurements.
基金supported by the Introduction Research Item of Northwest University for Nationalities(No.xbmuyjrc201110)the Fundamental Research Funds for the Central Universities(Nos.zyz2012062 and 31920130024)the Young and Middle-Aged Scientists Research Fund of Northwest University for Nationalities(No.12XB34)
文摘A two-photon fluorescent probe TPZn was developed for specific ratiometric imaging Zn2＋ in living cells and tissues. Significant ratiometric fluorescence change was based on photoinduced electron transfer and intramolecular charge transfer. The synthetic method of TPZn was simple. It was successfully used to selectively image Zn2＋ based on the higher binding affinity for Zn2＋ than for Cd2＋. TPZn was easily loaded into the living cell and tissues with high membrane permeability in a complex biological environment. TPZn could clearly visualize endogenous Zn2＋ by TP ratiometric imaging in hippocampal slices at a depth of 120 μm. Thus, TPZn is a useful tool to image of Zn2＋ in living cells and tissues without interference from Cd2＋.
基金the National Natural Science Foundation of China(Nos.21102148 and 21125205)National Basic Research Program of China(No.2011CB935800)the State Key Laboratory of Fine Chemicals,Department of Chemical Engineering,Dalian University of Technology for financial supports
文摘A new two-photon fluorescent probe, ADNO, for nitric oxide （NO） based on intramolecular photoinduced electron transfer （PET） mechanism d/splays a rapid response to NO with a remarkable fluorescent enhancement in PBS buffer. The excellent chemoselectivity of ADNO for NO over other ROS/RNS （reactive oxygen species or nitrogen species） and common metal ions was observed. Moreover, ADNO has been successfully applied in fluorescence imaging of NO of living cells using both one-photon microscopy （OPM） and two-~hoton microscopy （TPM）,
基金Project supported by National Key Basic Research Special Fund of China and by Natural Science Foundation of Beijing, China.
文摘The local density of photonic states （LDPS） of an infinite two-dimensional （2D） photonic crystal （PC） composed of rotated square-pillars in a 2D square lattice is calculated in terms of the plane-wave expansion method in a combination with the point group theory. The calculation results show that the LDPS strongly depends on the spatial positions. The variations of the LDPS as functions of the radial coordinate and frequency exhibit “mountain chain” structures with sharp peaks. The LDPS with large value spans a finite area and falls abruptly down to small value at the position corresponding to the interfaces between two different refractive index materials. The larger/lower LDPS occurs inward the lower/larger dielectric-constant medium. This feature can be well interpreted by the continuity of electricdisplacement vector at the interface. In the frequency range of the pseudo-PBG （photonic band gap）, the LDPS keeps very low value over the whole Wiger-Seitz cell. It indicates that the spontaneous emission in 2D PCs cannot be prohibited completely, but it can be inhibited intensively when the resonate frequency falls into the pseudo-PBG.
基金the National Natural Science Foundation of China(No.50273024)the Foundation for the Author of National Excellent Doctoral Dissertation of PR China(FANEDD,No 200333)+1 种基金Natural Foundation of Jiangsu Province(No.BK2003031)the Foundation of Jiangsu Province Education Committee(No.03KJB 150115)for financial support.
文摘Two novel V-shaped symmetric chromophores： E-2,8-bis（4-vinyl-4-carbazol-9-yl）diben- zothiophene （abbreviated as SK-G1） and E-2,8-bis（4-vinyl-4-triphenylamino） dibenzothiophene （abbreviated as ST-G1） have been synthesized and characterized. Their two photon absorption properties were measured by the open-aperture femtosecond Z-scan technique and the nanosecond nonlinear optical transmission （NLT）, respectively, when pumped by Ti： sapphire laser at 750 nm and 800 nm.
