A hydroponic experiment with six treatments, i.e., 0% seawater （control）, 10% seawater, 25% seawater, 0% seawater ＋ N （7.5 mmol L-1 NaNO3）, 10% seawater ＋ N （7.5 mmol L-1 NaO3）, and 25% seawater ＋ N （7.5 mmo...A hydroponic experiment with six treatments, i.e., 0% seawater （control）, 10% seawater, 25% seawater, 0% seawater ＋ N （7.5 mmol L-1 NaNO3）, 10% seawater ＋ N （7.5 mmol L-1 NaO3）, and 25% seawater ＋ N （7.5 mmol L-1 NaNO3）, was carried out to study the effect of nitrogen addition on the growth and physiological and biochemical characteristics of Jerusalem artichoke （Helianthus tuberosus） seedlings under seawater stress. The 10% seawater stress treatment had the least effect on plant growth while at 25% seawater growth was significantly inhibited. The malondialdehyde content and electrolyte leakage in leaves under 10% seawater were similar to those of the control, but significantly higher under the 25% seawater stress. The activities of superoxide dismutase, peroxidase and catalase in the leaves increased concomitantly with increasing seawater concentration and time. Proline and soluble-sugars in the leaves and Na^＋, K^＋, and Cl- contents in shoots and roots increased significantly with the concentration of seawater increasing. Nitrogen addition resulted in increasing fresh and dry weights of shoots and roots compared with seawater treatment without N. Nitrogen supplementation also significantly enhanced the activities of antioxidant enzymes in leaves. Addition of N to seawater enhanced the contents of proline and soluble-sugars in the leaves and K^＋ and total-N in the aerial parts and roots of H. tuberosus, but it resulted in declined concentrations of Na^＋ and Cl- in the aerial parts and roots. Nitrogen addition ameliorated the toxicity of seawater by improving the antioxidative enzymes, accumulating of proiine and soluble-sugars, and altering the distribution of inorganic ions in H. tuberosus.展开更多
An experiment with six treatments: CK1 (rainfed), CK2 (irrigated with freshwater), and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1:1, 1:2, 1:3, and 1:4 (v/v) ...An experiment with six treatments: CK1 (rainfed), CK2 (irrigated with freshwater), and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1:1, 1:2, 1:3, and 1:4 (v/v) was carried out during 2004 to assess the effect of saline aquaculture effluent on plant growth and soil properties in the Laizhou region, Shandong Province, China and to determine an optimal salinity threshold for aquaculture effluent. Cumulative evapotranspiration for the saline aquaculture effluent irrigation and non-irrigation treatments was lower than that for the freshwater irrigation treatment. Soil electrical conductivity was higher with respect to saline aquaculture effluent irrigation treatment compared to that with respect to non-irrigation or freshwater irrigation treatment. For Jerusalem artichoke (Helianthus tuberosus L.), in comparison to the freshwater treatment, plant height and aboveground biomass for the 1:3 and 1:4 treatments were constrained, whereas stem width and root biomass were enhanced. Concomitantly, higher tuber yield was obtained for the 1:3 and 1:4 treatments compared to that for CK1 and 1:1 treatments. Nitrogen and phosphorus were higher in tubers of the 1:4 treatment. This study demonstrated that saline aquaculture effluent could be used successfully to irrigate Jerusalem artichoke with higher tuber yield and nutrient removal.展开更多
Objective:To analyze the effects of feeding Helianthus tuberosus(HT) tubers on glucose tolerance and lipid profile in rats fed a high-fat diet(HFD). Methods:A normal HFD or HFD including 10 w/w% HT tubers(HFD + HT) wa...Objective:To analyze the effects of feeding Helianthus tuberosus(HT) tubers on glucose tolerance and lipid profile in rats fed a high-fat diet(HFD). Methods:A normal HFD or HFD including 10 w/w% HT tubers(HFD + HT) was fed to F334/Jcl rats. After 10 weeks,organ weights,glucose tolerance,and lipid profile were analyzed. Results:The body weight,liver weight,and epidermal fat content in the HFD group were higher than those of the normal group,and similar to those of the HFD + HT group. The oral glucose tolerance test at 10 weeks revealed that the blood glucose level 30 minutes after beginning the test in the HFD + HT group was significantly lower than that in the HFD group. Liver triglyceride and total cholesterol levels in the HFD + HT group were significantly lower than those in the HFD group. Fecal triglyceride and total cholesterol levels in the HFD + HT group were higher than those in the HFD group. Histological analyses revealed that fat and glycogen accumulation increased in the HFD group,but decreased in the HFD + HT group. Conclusions:These results indicate that HT tubers have anti-fatty liver effects based on improvements in glucose tolerance and the hepatic lipid profile.展开更多
With Qingyu 1 and Qingyu 2 as test materials, variation of soluble carbohydrate content in different parts were studied under two types of artificially simulated drought stress （polyethylene glycd PEG-6000 stress and...With Qingyu 1 and Qingyu 2 as test materials, variation of soluble carbohydrate content in different parts were studied under two types of artificially simulated drought stress （polyethylene glycd PEG-6000 stress and water-controlling stress）. The results showed that under the stress of PEG, soluble carbohydrate content in leaves of Qingyu 1 and Qingyu 2 increased with the prolonging time of stress; soluble carbohydrate content in stems of Qingyu 1 did not show regular changes with the prolonging time of stress, while 30% PEG stress received the best effect on Qingyu 2 after 24 h; soluble carbohydrate content in roots of Qingyu 1 and Qingyu 2 increased with the prolonging time of stress. Under different intensities of water-controUing stress, soluble carbohydrate content in leaves of Qingyu 1 first increased then decreased, while that in leaves of Qingyu 2 increased; soluble carbohydrate content in stems of Qingyu 1 and Qingyu 2 increased with the pro- longing time of water-controlling stress; soluble carbohydrate content in roots of Qingyu 1 decreased with the prolonging time of water-contmlling stress, while that in roots of Qingyu 2 differed slightly.展开更多
Objective:This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus(U.tuberosus) using in vitro models.Methods:Lyophilized pulp and acetone extracts of U.tuberosus were...Objective:This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus(U.tuberosus) using in vitro models.Methods:Lyophilized pulp and acetone extracts of U.tuberosus were produced using ultrasound extraction.The capacity for collagenase activation was evaluated using fluorescence detection of the enzymatic activity.Then,the influence of U.tuberosus extracts on cell proliferation,cell migration and synthesis of the extracellular matrix(ECM)proteins,metalloproteinase(MMP-1) and pro-collagen was analyzed using human dermal fibroblasts in culture.Results:An increase in collagenase activity of 12% supports the utility of U.tuberosus as an agent for scar treatment.In addition,the extracts showed an increase in the proliferation and migration of human dermal fibroblasts and the production of pro-collagen and MMP-1 after treatment with U.tuberosus extracts.The increase in proliferation,migration and pro-collagen levels positively influenced the regeneration of scarless tissue during the proliferation phase,whereas the increase in MMP-1 may have favored the wound healing process during the remodeling and cellular differentiation phases.Conclusion:The results of this study show for first time that U.tuberosus is a promising candidate to support scarless tissue regeneration.展开更多
By growth rate method, the inhibitory effects of five solvent extracts from Helianthus tuberosus leaves against Phytophthora capsici were studied in the test. The results showed that different solvent extracts all had...By growth rate method, the inhibitory effects of five solvent extracts from Helianthus tuberosus leaves against Phytophthora capsici were studied in the test. The results showed that different solvent extracts all had inhibitory effect against P. capsici, while 12.5 mg/mL of extracts from H. tuberosus leaves with petroleum ether and ethyl acetate as solvents had the highest inhibitory effect against P. capsici, reaching 100%. In case of various solvent extracts with different concentration gradients, ethyl acetate extract had the most significant inhibitory effect; when the concentration was 5 mg/mL, the inhibitory effect of ethyl acetate extract had reached 100% ; when the concentration reduced to 2.5 mg/mL, the inhibitory effect was still （27.91 ±2. 076） %, significantly higher than that of other solvent extracts at the same concentration. The 50 times dilution of ethyl acetate extract from H. tuberosus leaves was selected for pot test against pepper blight. , and the results showed that its control effect against pepper blight reached 100.00%, superior than that of chemical agent 25% metalaxyl WP 400 times dilution.展开更多
[Objective] The research aimed to study the adsorption characteristics of the Helianthus tuberosus stems to methylene blue in water. [Method] The optimum condition, adsorption thermodynamic and kinetic characteristics...[Objective] The research aimed to study the adsorption characteristics of the Helianthus tuberosus stems to methylene blue in water. [Method] The optimum condition, adsorption thermodynamic and kinetic characteristics were studied on the methylene blue adsorbed by Helianthus tuberosus stems. [ Result] The equilibrium process was described well by the Langmuir isotherm model. The thermodynamics parameters were enthalpy changes （△H） of -12.147 kJ/mol, Gibb＇S free energy changes （△G） of -25.75 k J/reel, and entropy changes （△S） of 47.21 J/（mol · K）, respectively, at 288 K, indicating that the adsorption thermodynamic of methylene blue adsorbed by helianthus tuberoses stems was a spontaneous and exothermic process. The kinetics of the interactions showed better agreement with the Lagergren second order kinetics. The apparent activation energy （Ea） of adsorption process was 271.7 kJ/mol. [ Conclusion] This study provided the theoretical basis for the development and utilization of low-cost agricultural wastes to remove the hazardous substances in industrial wastewater.展开更多
Pachyrhizus tuberosus is a native plant of short life cycle found in South America riverside, which provides easy starch extraction from its tuberous roots. The aim of this study was to determine the physicochemical, ...Pachyrhizus tuberosus is a native plant of short life cycle found in South America riverside, which provides easy starch extraction from its tuberous roots. The aim of this study was to determine the physicochemical, rheological and functionality of the starch granules extracted from the roots of five phenotypes identified as V2, V3, V4, V6 and V7. Protein and ash content of all phenotypes were considerable high when compared to other root sources such as cassava varying from 4.35% to 7.43% and 1.58% to 2.49%, respectively, whereas lipid content was lower, between 0.29 and 0.49%. The starch granules were mostly circular and polygonal with varied sizes. The starch granules structural conformation showed cristallinity A type, normally for cereals. The maximum pasting viscosity at 95℃ ranged from 1644 cP (V7) to 2232 cP (V2). The initial temperature of pasting formation occurred at 69.4℃ for V2, 71.5℃ for V3, 87.9℃ for V4, 69.5℃ for V6 and 71.5℃ for V7. These values showed high variability within the phenotypes and generally high for roots and tubers starches. The maximum viscosity at 95℃ for V2, V3, V4, V6 and V7 were 2232, 2150, 1995, 2214 and 1644 cP, respectively. The viscosity curves showed low tendency to retrogradation. The thermal properties showed that the enthalpy of gelatinization varied from 8.91 J/g (V3) to 11.78 J/g (V2). The initial gelatinization temperature varied from 63.19℃ (V6) to 65.14℃ (V4). The swelling power at 90℃ ranged from 14.7% to 20.1% p/p and solubility from 10.3% (V2) to 27.2% (V7). It is concluded that Pachyrhizus tuberosus starch showed low retrogradation (1320 - 1560 cP) comparable to non-common native waxy starches, a feature which indicates the capability of using this natural and easy extraction starch source as gelling agent in certain manufactured food of undesirable retrogradation.展开更多
Atomic absorption spectrophotometry,sulfuric acid and potassium dichromate oxidation method and ultraviolet spectrophotometry,were used to determine mineral elements,fiber,the content of chlorogenic acid and flavones ...Atomic absorption spectrophotometry,sulfuric acid and potassium dichromate oxidation method and ultraviolet spectrophotometry,were used to determine mineral elements,fiber,the content of chlorogenic acid and flavones in leaves of 24 Helianthus tuberosus Linn.resources,and the characteristics of the leaf introduced from abroad were evaluated.The results showed that the highest water content of 22 species was F12(27.58%),and the lowest was F16(19.02%).The difference in mean water content between the species from Denmark and France was small,but it was lower than that of Qingyu 3 and Qingyu 4.There were 3 orbicular leaves,1 long oval-shaped leaf and 18 oval leaves.The highest K content in the leaves was F19(30.62 mg/g),which was 2.5 times than Qingyu 3.The highest Mg content was D8(14.17 mg/g).The Fe content hadlittledifference,ranging from 0.09 mg/g to 0.19 mg/g.The highest Ca content was D8(26.87 mg/g).The highest level of chlorogenic acid and flavones was F7,2.55%and 1.24 g/100 g respectively.The highest fiber content was F9(16.7%),and the lowest was F19(7.36%).Through the analysis of the main component and the clustering analysis,when the genetic distance was 0.65,the 24 resources can be divided into three major categories.The first category of leaves were mainly oval and orbicular;the second category of leaves were long oval-shaped;the third category of leaves were oval.There was a difference between the various indexes in the leaf of different kinds of H.tuberosus Linn.resources.Finally,F19,D8,F9,F7,D14 and D11,6 specific species(F19,D8,F9,F7,D14 and D11)were screened out for further studies in the future.展开更多
Herein, we report the cloning and molecular characterization of a full cDNA encoding a putative plastidic ATP/ADP transporter, designated HtAATP, for Helianthus tuberosus L. The ATP/ADP translocator protein was isolat...Herein, we report the cloning and molecular characterization of a full cDNA encoding a putative plastidic ATP/ADP transporter, designated HtAATP, for Helianthus tuberosus L. The ATP/ADP translocator protein was isolated from the tuber-cDNA library of H. tuberosus for the first time. The predicted HtAATP protein was judged as a plastidic ATP/ADP translocator protein from its high homology at the amino acid sequence level to the two Arabidopsis thaliana plastidic ATP/ADP translocator proteins AATP1 and AATP2 （84.8% and 79.9% identity, respectively）. Amino acid sequence analysis of the primary structure of HtAATP revealed that it belonged to the plastidic ATP/ADP transporter family. Hydropathy prediction indicated that HtAATP gene product is a highly hydrophobic membrane protein that contains 10 transmembrane domains to form a spanning topology. Southern blotting analysis showed that the HtAATP gene is a single-copy gene in the H. tuberosus genome. Tissue distribution analysis showed that the HtAATP gene is prominently expressed in sink tissues. A stable expression pattern in tubers at different developmental stages implies an active involvement of HtAATP during carbohydrate formation.展开更多
In order to find new insect resistance genes, four homologous cDNAs, hta-a, hta-b, hta-c and hta-d with lengths of 775, 718, 784 and 752 bp, respectively （GenBank accession numbers AF477031-AF477034）, were iso...In order to find new insect resistance genes, four homologous cDNAs, hta-a, hta-b, hta-c and hta-d with lengths of 775, 718, 784 and 752 bp, respectively （GenBank accession numbers AF477031-AF477034）, were isolated from a tuber cDNA expression library of Helianthus tuberosus L. Sequence analysis revealed that all four cDNAs contain an open reading frame of 444 bp, coding a polypeptide of 147 amino acid residues, and that the sequences of the cDNAs are very similar to those of the mannose-binding agglutinin genes of the jacalin-related family. In hemagglutination reactions and hapten inhibition assays, affinity-purified HTA （Helianthus tuberosus agglutinin） from induced Escherichia coli BL21（DE3） expressing GST-HTA shows hemagglutination ability and a higher carbohydrate-binding ability for mannose than other tested sugars. Trypsin inhibitory activity was detected in the crude extracts of induced E. coli BL21（DE3） expressing HTA, and was further verified by trypsin inhibitory activity staining on native polyacrylamide gel. The mechanism of interaction between HTA and trypsin was studied by molecular modeling. We found that plenty of hydrogen bonds and electrostatic interactions can be formed between the supposed binding sites of HTA-b and the active site of trypsin, and that a stable HTA/trypsin complex can be formed. The results above imply that HTA might be a bifunctional protein with carbohydrate-binding activity and trypsin inhibitory activity. Moreover, Northern blotting analysis demonstrated that hta is predominantly expressed in tubers of H. tuberosus, very weakly expressed in stems, but not expressed at all in other tissues. Southern blotting analysis indicated that hta is encoded by a multi-gene family. The insect resistance traits have been described in another paper.展开更多
Three treatments consisting of 0%,15%,and 30%seawater were investigated to analyse the ecotypic variabilities among five populations of Jerusalem artichoke(Helianthus tuberosus)regarding their responses to seawater st...Three treatments consisting of 0%,15%,and 30%seawater were investigated to analyse the ecotypic variabilities among five populations of Jerusalem artichoke(Helianthus tuberosus)regarding their responses to seawater stress under a hydroponic culture system.Analyses were done 2,4,and 6 days after treatments.The 15%and 30%seawater treatments reduced the growth rates of roots and shoots of H.tuberosus populations.The activities of superoxide dismutase, peroxidase,and catalase majored in the leaves were stimulated under the seawater stress.The electrolyte leakage and malondialdehyde contents of the leaves were also stimulated owing to seawater stress.The contents of proline and soluble- sugars in the leaves increased significantly with increasing seawater concentrations.The concentrations of Na+,K+,and Cl-in the aerial parts and roots increased with an increase in the seawater concentration throughout the experimental period.There were ecotypic differences among the five populations of H.tuberosus as evidenced by the analyses of the above items in both aerial parts and roots under seawater treatment.The magnitude of the ecotypic variance components indicated that a substantial proportion of the total variation for these physiological and biochemical responses were owing to ecotype,indicating the possibility of improvement through hybridization and selection.展开更多
基金Project supported by the National Science and Technology Ministry (Nos.2006BAD09A08-03-01 and 2006BAD09A04-05)the National High Technology Research and Development Program (863 Program) of China (No.2007AA091702)the Opening Program of Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresource and Environmental Protection (JLCBE) (No.JLCBE07001).
文摘A hydroponic experiment with six treatments, i.e., 0% seawater （control）, 10% seawater, 25% seawater, 0% seawater ＋ N （7.5 mmol L-1 NaNO3）, 10% seawater ＋ N （7.5 mmol L-1 NaO3）, and 25% seawater ＋ N （7.5 mmol L-1 NaNO3）, was carried out to study the effect of nitrogen addition on the growth and physiological and biochemical characteristics of Jerusalem artichoke （Helianthus tuberosus） seedlings under seawater stress. The 10% seawater stress treatment had the least effect on plant growth while at 25% seawater growth was significantly inhibited. The malondialdehyde content and electrolyte leakage in leaves under 10% seawater were similar to those of the control, but significantly higher under the 25% seawater stress. The activities of superoxide dismutase, peroxidase and catalase in the leaves increased concomitantly with increasing seawater concentration and time. Proline and soluble-sugars in the leaves and Na^＋, K^＋, and Cl- contents in shoots and roots increased significantly with the concentration of seawater increasing. Nitrogen addition resulted in increasing fresh and dry weights of shoots and roots compared with seawater treatment without N. Nitrogen supplementation also significantly enhanced the activities of antioxidant enzymes in leaves. Addition of N to seawater enhanced the contents of proline and soluble-sugars in the leaves and K^＋ and total-N in the aerial parts and roots of H. tuberosus, but it resulted in declined concentrations of Na^＋ and Cl- in the aerial parts and roots. Nitrogen addition ameliorated the toxicity of seawater by improving the antioxidative enzymes, accumulating of proiine and soluble-sugars, and altering the distribution of inorganic ions in H. tuberosus.
基金Project supported by the National Natural Science Foundation of China (No. 30470331)
文摘An experiment with six treatments: CK1 (rainfed), CK2 (irrigated with freshwater), and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1:1, 1:2, 1:3, and 1:4 (v/v) was carried out during 2004 to assess the effect of saline aquaculture effluent on plant growth and soil properties in the Laizhou region, Shandong Province, China and to determine an optimal salinity threshold for aquaculture effluent. Cumulative evapotranspiration for the saline aquaculture effluent irrigation and non-irrigation treatments was lower than that for the freshwater irrigation treatment. Soil electrical conductivity was higher with respect to saline aquaculture effluent irrigation treatment compared to that with respect to non-irrigation or freshwater irrigation treatment. For Jerusalem artichoke (Helianthus tuberosus L.), in comparison to the freshwater treatment, plant height and aboveground biomass for the 1:3 and 1:4 treatments were constrained, whereas stem width and root biomass were enhanced. Concomitantly, higher tuber yield was obtained for the 1:3 and 1:4 treatments compared to that for CK1 and 1:1 treatments. Nitrogen and phosphorus were higher in tubers of the 1:4 treatment. This study demonstrated that saline aquaculture effluent could be used successfully to irrigate Jerusalem artichoke with higher tuber yield and nutrient removal.
基金supported by the Tokushima prefectural government
文摘Objective:To analyze the effects of feeding Helianthus tuberosus(HT) tubers on glucose tolerance and lipid profile in rats fed a high-fat diet(HFD). Methods:A normal HFD or HFD including 10 w/w% HT tubers(HFD + HT) was fed to F334/Jcl rats. After 10 weeks,organ weights,glucose tolerance,and lipid profile were analyzed. Results:The body weight,liver weight,and epidermal fat content in the HFD group were higher than those of the normal group,and similar to those of the HFD + HT group. The oral glucose tolerance test at 10 weeks revealed that the blood glucose level 30 minutes after beginning the test in the HFD + HT group was significantly lower than that in the HFD group. Liver triglyceride and total cholesterol levels in the HFD + HT group were significantly lower than those in the HFD group. Fecal triglyceride and total cholesterol levels in the HFD + HT group were higher than those in the HFD group. Histological analyses revealed that fat and glycogen accumulation increased in the HFD group,but decreased in the HFD + HT group. Conclusions:These results indicate that HT tubers have anti-fatty liver effects based on improvements in glucose tolerance and the hepatic lipid profile.
基金Supported by Special Promotion Plan of Innovation Team of Qinghai University(2014-NKY-209)&Special Project for Kunlun Scholars of Qinghai University&Special Project of Qinghai Provincial Key Laboratory(2015-Z-Y13)
文摘With Qingyu 1 and Qingyu 2 as test materials, variation of soluble carbohydrate content in different parts were studied under two types of artificially simulated drought stress （polyethylene glycd PEG-6000 stress and water-controlling stress）. The results showed that under the stress of PEG, soluble carbohydrate content in leaves of Qingyu 1 and Qingyu 2 increased with the prolonging time of stress; soluble carbohydrate content in stems of Qingyu 1 did not show regular changes with the prolonging time of stress, while 30% PEG stress received the best effect on Qingyu 2 after 24 h; soluble carbohydrate content in roots of Qingyu 1 and Qingyu 2 increased with the prolonging time of stress. Under different intensities of water-controUing stress, soluble carbohydrate content in leaves of Qingyu 1 first increased then decreased, while that in leaves of Qingyu 2 increased; soluble carbohydrate content in stems of Qingyu 1 and Qingyu 2 increased with the pro- longing time of water-controlling stress; soluble carbohydrate content in roots of Qingyu 1 decreased with the prolonging time of water-contmlling stress, while that in roots of Qingyu 2 differed slightly.
基金supported by the Universidad de Antioquia CODI-Estrategia de Sostenibilidad 2016-2017 GISB
文摘Objective:This study was designed to investigate the wound healing activity of aqueous extracts of Ullucus tuberosus(U.tuberosus) using in vitro models.Methods:Lyophilized pulp and acetone extracts of U.tuberosus were produced using ultrasound extraction.The capacity for collagenase activation was evaluated using fluorescence detection of the enzymatic activity.Then,the influence of U.tuberosus extracts on cell proliferation,cell migration and synthesis of the extracellular matrix(ECM)proteins,metalloproteinase(MMP-1) and pro-collagen was analyzed using human dermal fibroblasts in culture.Results:An increase in collagenase activity of 12% supports the utility of U.tuberosus as an agent for scar treatment.In addition,the extracts showed an increase in the proliferation and migration of human dermal fibroblasts and the production of pro-collagen and MMP-1 after treatment with U.tuberosus extracts.The increase in proliferation,migration and pro-collagen levels positively influenced the regeneration of scarless tissue during the proliferation phase,whereas the increase in MMP-1 may have favored the wound healing process during the remodeling and cellular differentiation phases.Conclusion:The results of this study show for first time that U.tuberosus is a promising candidate to support scarless tissue regeneration.
基金Supported by Applied Basic Research Project of Qinghai Provincial Science and Technology Department(2014-ZJ-735)
文摘By growth rate method, the inhibitory effects of five solvent extracts from Helianthus tuberosus leaves against Phytophthora capsici were studied in the test. The results showed that different solvent extracts all had inhibitory effect against P. capsici, while 12.5 mg/mL of extracts from H. tuberosus leaves with petroleum ether and ethyl acetate as solvents had the highest inhibitory effect against P. capsici, reaching 100%. In case of various solvent extracts with different concentration gradients, ethyl acetate extract had the most significant inhibitory effect; when the concentration was 5 mg/mL, the inhibitory effect of ethyl acetate extract had reached 100% ; when the concentration reduced to 2.5 mg/mL, the inhibitory effect was still （27.91 ±2. 076） %, significantly higher than that of other solvent extracts at the same concentration. The 50 times dilution of ethyl acetate extract from H. tuberosus leaves was selected for pot test against pepper blight. , and the results showed that its control effect against pepper blight reached 100.00%, superior than that of chemical agent 25% metalaxyl WP 400 times dilution.
基金Supported by the National Training Programs of Innovation and Entrepreneurship for Undergraduates(201210719011)the Key Program of Natural Science Foundation of Yan'an University(YDZ2012-09)
文摘[Objective] The research aimed to study the adsorption characteristics of the Helianthus tuberosus stems to methylene blue in water. [Method] The optimum condition, adsorption thermodynamic and kinetic characteristics were studied on the methylene blue adsorbed by Helianthus tuberosus stems. [ Result] The equilibrium process was described well by the Langmuir isotherm model. The thermodynamics parameters were enthalpy changes （△H） of -12.147 kJ/mol, Gibb＇S free energy changes （△G） of -25.75 k J/reel, and entropy changes （△S） of 47.21 J/（mol · K）, respectively, at 288 K, indicating that the adsorption thermodynamic of methylene blue adsorbed by helianthus tuberoses stems was a spontaneous and exothermic process. The kinetics of the interactions showed better agreement with the Lagergren second order kinetics. The apparent activation energy （Ea） of adsorption process was 271.7 kJ/mol. [ Conclusion] This study provided the theoretical basis for the development and utilization of low-cost agricultural wastes to remove the hazardous substances in industrial wastewater.
文摘Pachyrhizus tuberosus is a native plant of short life cycle found in South America riverside, which provides easy starch extraction from its tuberous roots. The aim of this study was to determine the physicochemical, rheological and functionality of the starch granules extracted from the roots of five phenotypes identified as V2, V3, V4, V6 and V7. Protein and ash content of all phenotypes were considerable high when compared to other root sources such as cassava varying from 4.35% to 7.43% and 1.58% to 2.49%, respectively, whereas lipid content was lower, between 0.29 and 0.49%. The starch granules were mostly circular and polygonal with varied sizes. The starch granules structural conformation showed cristallinity A type, normally for cereals. The maximum pasting viscosity at 95℃ ranged from 1644 cP (V7) to 2232 cP (V2). The initial temperature of pasting formation occurred at 69.4℃ for V2, 71.5℃ for V3, 87.9℃ for V4, 69.5℃ for V6 and 71.5℃ for V7. These values showed high variability within the phenotypes and generally high for roots and tubers starches. The maximum viscosity at 95℃ for V2, V3, V4, V6 and V7 were 2232, 2150, 1995, 2214 and 1644 cP, respectively. The viscosity curves showed low tendency to retrogradation. The thermal properties showed that the enthalpy of gelatinization varied from 8.91 J/g (V3) to 11.78 J/g (V2). The initial gelatinization temperature varied from 63.19℃ (V6) to 65.14℃ (V4). The swelling power at 90℃ ranged from 14.7% to 20.1% p/p and solubility from 10.3% (V2) to 27.2% (V7). It is concluded that Pachyrhizus tuberosus starch showed low retrogradation (1320 - 1560 cP) comparable to non-common native waxy starches, a feature which indicates the capability of using this natural and easy extraction starch source as gelling agent in certain manufactured food of undesirable retrogradation.
文摘Atomic absorption spectrophotometry,sulfuric acid and potassium dichromate oxidation method and ultraviolet spectrophotometry,were used to determine mineral elements,fiber,the content of chlorogenic acid and flavones in leaves of 24 Helianthus tuberosus Linn.resources,and the characteristics of the leaf introduced from abroad were evaluated.The results showed that the highest water content of 22 species was F12(27.58%),and the lowest was F16(19.02%).The difference in mean water content between the species from Denmark and France was small,but it was lower than that of Qingyu 3 and Qingyu 4.There were 3 orbicular leaves,1 long oval-shaped leaf and 18 oval leaves.The highest K content in the leaves was F19(30.62 mg/g),which was 2.5 times than Qingyu 3.The highest Mg content was D8(14.17 mg/g).The Fe content hadlittledifference,ranging from 0.09 mg/g to 0.19 mg/g.The highest Ca content was D8(26.87 mg/g).The highest level of chlorogenic acid and flavones was F7,2.55%and 1.24 g/100 g respectively.The highest fiber content was F9(16.7%),and the lowest was F19(7.36%).Through the analysis of the main component and the clustering analysis,when the genetic distance was 0.65,the 24 resources can be divided into three major categories.The first category of leaves were mainly oval and orbicular;the second category of leaves were long oval-shaped;the third category of leaves were oval.There was a difference between the various indexes in the leaf of different kinds of H.tuberosus Linn.resources.Finally,F19,D8,F9,F7,D14 and D11,6 specific species(F19,D8,F9,F7,D14 and D11)were screened out for further studies in the future.
文摘Herein, we report the cloning and molecular characterization of a full cDNA encoding a putative plastidic ATP/ADP transporter, designated HtAATP, for Helianthus tuberosus L. The ATP/ADP translocator protein was isolated from the tuber-cDNA library of H. tuberosus for the first time. The predicted HtAATP protein was judged as a plastidic ATP/ADP translocator protein from its high homology at the amino acid sequence level to the two Arabidopsis thaliana plastidic ATP/ADP translocator proteins AATP1 and AATP2 （84.8% and 79.9% identity, respectively）. Amino acid sequence analysis of the primary structure of HtAATP revealed that it belonged to the plastidic ATP/ADP transporter family. Hydropathy prediction indicated that HtAATP gene product is a highly hydrophobic membrane protein that contains 10 transmembrane domains to form a spanning topology. Southern blotting analysis showed that the HtAATP gene is a single-copy gene in the H. tuberosus genome. Tissue distribution analysis showed that the HtAATP gene is prominently expressed in sink tissues. A stable expression pattern in tubers at different developmental stages implies an active involvement of HtAATP during carbohydrate formation.
基金Supported by the State Key Basic Research and Development Plan of China(G2000016205)the Hi-Tech Research and Development(863) Program of China(2001AA212041)+2 种基金the National Special Program for Research and Industrialization of Transgenic Plants(J99-B-004)the Rockefeller Foundation,the International Program on Rice Biotechnology(RF 97042 #14)the Special Supporting Program of the Chinese Academy of Sciences(STZ98-3-08)
文摘In order to find new insect resistance genes, four homologous cDNAs, hta-a, hta-b, hta-c and hta-d with lengths of 775, 718, 784 and 752 bp, respectively （GenBank accession numbers AF477031-AF477034）, were isolated from a tuber cDNA expression library of Helianthus tuberosus L. Sequence analysis revealed that all four cDNAs contain an open reading frame of 444 bp, coding a polypeptide of 147 amino acid residues, and that the sequences of the cDNAs are very similar to those of the mannose-binding agglutinin genes of the jacalin-related family. In hemagglutination reactions and hapten inhibition assays, affinity-purified HTA （Helianthus tuberosus agglutinin） from induced Escherichia coli BL21（DE3） expressing GST-HTA shows hemagglutination ability and a higher carbohydrate-binding ability for mannose than other tested sugars. Trypsin inhibitory activity was detected in the crude extracts of induced E. coli BL21（DE3） expressing HTA, and was further verified by trypsin inhibitory activity staining on native polyacrylamide gel. The mechanism of interaction between HTA and trypsin was studied by molecular modeling. We found that plenty of hydrogen bonds and electrostatic interactions can be formed between the supposed binding sites of HTA-b and the active site of trypsin, and that a stable HTA/trypsin complex can be formed. The results above imply that HTA might be a bifunctional protein with carbohydrate-binding activity and trypsin inhibitory activity. Moreover, Northern blotting analysis demonstrated that hta is predominantly expressed in tubers of H. tuberosus, very weakly expressed in stems, but not expressed at all in other tissues. Southern blotting analysis indicated that hta is encoded by a multi-gene family. The insect resistance traits have been described in another paper.
基金Project Supported by the National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science&Technology of China(Nos.2006BAD09A08-03-01 and 2006BAD09A04-05)the National High Technology Research and Development Program(863 Program)of China(No.2007AA091702)the Opening Program of Jiangsu Provincial Key Laboratory of Coastal Wetland Bio-resource and Environmental Protection(JLCBE)(No.JLCBE07001)
文摘Three treatments consisting of 0%,15%,and 30%seawater were investigated to analyse the ecotypic variabilities among five populations of Jerusalem artichoke(Helianthus tuberosus)regarding their responses to seawater stress under a hydroponic culture system.Analyses were done 2,4,and 6 days after treatments.The 15%and 30%seawater treatments reduced the growth rates of roots and shoots of H.tuberosus populations.The activities of superoxide dismutase, peroxidase,and catalase majored in the leaves were stimulated under the seawater stress.The electrolyte leakage and malondialdehyde contents of the leaves were also stimulated owing to seawater stress.The contents of proline and soluble- sugars in the leaves increased significantly with increasing seawater concentrations.The concentrations of Na+,K+,and Cl-in the aerial parts and roots increased with an increase in the seawater concentration throughout the experimental period.There were ecotypic differences among the five populations of H.tuberosus as evidenced by the analyses of the above items in both aerial parts and roots under seawater treatment.The magnitude of the ecotypic variance components indicated that a substantial proportion of the total variation for these physiological and biochemical responses were owing to ecotype,indicating the possibility of improvement through hybridization and selection.
