近年来,随着新一代测序技术的发展,大量具有独特功能的非编码RNA及其生物学功能逐渐被证实,包括microRNA(miRNA)[1]、piwi相互作用RNA(piRNA)[2]、长链非编码RNA(lncRNA)[3]和环状RNA(circRNA)[4]等。其中,转运RNA衍生的小RNA(transfer ...近年来,随着新一代测序技术的发展,大量具有独特功能的非编码RNA及其生物学功能逐渐被证实,包括microRNA(miRNA)[1]、piwi相互作用RNA(piRNA)[2]、长链非编码RNA(lncRNA)[3]和环状RNA(circRNA)[4]等。其中,转运RNA衍生的小RNA(transfer RNA-derived small RNA,tsRNAs),一种由tRNA前体(pre-tRNA)或成熟tRNAs被特异性核酸酶切割而成的新型非编码小RNA,被证实广泛存在于原核和真核生物转录组中。展开更多
转运RNA衍生的小RNA(tRNA-derived small RNA,tsRNA)是由前体或成熟tRNA经特异性核酸内切酶切割产生的非编码小RNA,具有多种生物学功能。在妇科疾病领域,tsRNA被证实在卵巢癌、子宫内膜癌、子宫内膜异位症等疾病中差异表达,并与疾病的...转运RNA衍生的小RNA(tRNA-derived small RNA,tsRNA)是由前体或成熟tRNA经特异性核酸内切酶切割产生的非编码小RNA,具有多种生物学功能。在妇科疾病领域,tsRNA被证实在卵巢癌、子宫内膜癌、子宫内膜异位症等疾病中差异表达,并与疾病的发生、发展及预后密切相关。文章通过总结tsRNA在妇科疾病中的研究进展,旨在为其机制研究、早期诊断和精准治疗提供新思路。展开更多
转运RNA(transfer RNA,tRNA)衍生的小RNA(tRNA-derived small RNA,tsRNA)是一类新近发现的重要的非编码RNA分子。最近的研究表明,tsRNA在胃癌、结直肠癌等胃肠道肿瘤中表达水平发生显著变化。这种疾病相关的表达特征,使tsRNA有望成为这...转运RNA(transfer RNA,tRNA)衍生的小RNA(tRNA-derived small RNA,tsRNA)是一类新近发现的重要的非编码RNA分子。最近的研究表明,tsRNA在胃癌、结直肠癌等胃肠道肿瘤中表达水平发生显著变化。这种疾病相关的表达特征,使tsRNA有望成为这些疾病诊断和治疗的新型生物标志物。本文综述了tsRNA的来源、分类及其生物学功能,同时探讨tsRNA在胃癌、结直肠癌中的表达变化特点,为这些疾病的诊断和治疗提供了新思路。展开更多
Increasing evidences have highlighted the significant association between tsRNAs and diseases.Predicting potential tsRNA-disease associations based on computational methods can effectively reduce human and resource co...Increasing evidences have highlighted the significant association between tsRNAs and diseases.Predicting potential tsRNA-disease associations based on computational methods can effectively reduce human and resource consumption.However,there is a scarcity of computational methods for predicting tsRNA-disease associations.Therefore,we propose Contrastive Learning-based prediction of tsRNA-Disease Associations(CLTDA).It reconstructs known associations between tsRNAs and diseases based on adaptive Singular Value Decomposition(SVD).Then,we employ Graph Convolutional Networks(GCNs)for feature extraction from both the original and reconstructed tsRNA-disease associations,and optimize the GCNs by using contrastive learning loss and Bayesian Personalized Ranking(BPR)loss.In addition,the Bayesian negative sampling method is used to select high-quality negative samples for learning the features of tsRNA and disease.Finally,a Multi-Layer Perceptron(MLP)is utilized to calculates the score of potential association.We conduct five-fold cross-validation and denovo experiments on a manually collected tsRNA-disease association dataset,and the experimental results show that CLTDA outperforms the other six state-of-the-art methods.We also perform a case study on lung cancer and experimental results show that CLTDA is an effective tool for predicting potential associations between tsRNAs and diseases.展开更多
Transfer RNA-derived small RNAs(tsRNAs),a class of non-coding RNAs derived from precursor or mature tRNAs,are now recognized as critical regulators in response to cellular stress.tsRNAs exhibit differential expression...Transfer RNA-derived small RNAs(tsRNAs),a class of non-coding RNAs derived from precursor or mature tRNAs,are now recognized as critical regulators in response to cellular stress.tsRNAs exhibit differential expression during brain aging and in age-related neurodegenerative disorders such as Alzheimer’s disease(AD),suggesting their involvement in the molecular processes underlying neuronal aging and degeneration.This article summarizes recent advances in our understanding of tsRNA biogenesis,classification,and function,emphasizing their regulatory role in brain aging and AD pathology.We also highlight the diagnostic and therapeutic implications of tsRNAs and discuss future directions for exploring their mechanistic and clinical relevance.展开更多
越来越多的证据表明,上一代在环境压力下产生的某些获得性性状可以"记忆"在配子中,并以一种不依赖DNA序列的方式传递给下一代,这种现象被称为获得性遗传。然而,关于精子中介导获得性遗传现象的分子机制尚不清楚。近年来,随着...越来越多的证据表明,上一代在环境压力下产生的某些获得性性状可以"记忆"在配子中,并以一种不依赖DNA序列的方式传递给下一代,这种现象被称为获得性遗传。然而,关于精子中介导获得性遗传现象的分子机制尚不清楚。近年来,随着精子RNA领域的发展,我们实验室率先发现精子中一类来源于成熟tRNA的小RNA (tRNA-derived small RNAs, tsRNAs)及其RNA修饰可以作为一种新型的表观遗传信息载体,介导父代获得性代谢紊乱性状向子代传递。此外,我们进一步发现了调控精子RNA介导获得性遗传的关键分子——tRNA甲基转移酶DNMT2,从RNA修饰及修饰酶的角度为获得性遗传的机制研究打开了新思路。鉴于该领域的迅速发展,该文拟从精子tsRNAs、RNA修饰以及DNMT2的角度综述近年来精子RNA及RNA修饰介导的获得性遗传机制的研究进展。展开更多
为了解拟南芥中Dicer-like蛋白对tRNA衍生的小RNA(tRNA-derived small RNAs,tsRNAs)的产生有何影响,对拟南芥野生型和不同Dicer-like(DCL)基因突变体进行tRNA-seq测序,并分析tsRNA和tRNA的表达量.结果显示,DCL4基因突变后tsRNA的表达量...为了解拟南芥中Dicer-like蛋白对tRNA衍生的小RNA(tRNA-derived small RNAs,tsRNAs)的产生有何影响,对拟南芥野生型和不同Dicer-like(DCL)基因突变体进行tRNA-seq测序,并分析tsRNA和tRNA的表达量.结果显示,DCL4基因突变后tsRNA的表达量明显降低,说明DCL4可能参与tsRNA的产生.拟南芥tRC1位点(Chr1:21268000-21310000)具有大量串联分布的tRNA序列,通过对RNA介导的甲基化(RdDM)途径相关基因CLSY1突变体中tRC1位点的24 nt siRNA和tsRNA进行分析,推断tRC1位点的tsRNA受RdDM途径负调控.综上,本研究鉴定到DCL4在tsRNA生成中的潜在作用,部分tsRNA的生成与RdDM途径有关.展开更多
文摘近年来,随着新一代测序技术的发展,大量具有独特功能的非编码RNA及其生物学功能逐渐被证实,包括microRNA(miRNA)[1]、piwi相互作用RNA(piRNA)[2]、长链非编码RNA(lncRNA)[3]和环状RNA(circRNA)[4]等。其中,转运RNA衍生的小RNA(transfer RNA-derived small RNA,tsRNAs),一种由tRNA前体(pre-tRNA)或成熟tRNAs被特异性核酸酶切割而成的新型非编码小RNA,被证实广泛存在于原核和真核生物转录组中。
文摘转运RNA衍生的小RNA(tRNA-derived small RNA,tsRNA)是由前体或成熟tRNA经特异性核酸内切酶切割产生的非编码小RNA,具有多种生物学功能。在妇科疾病领域,tsRNA被证实在卵巢癌、子宫内膜癌、子宫内膜异位症等疾病中差异表达,并与疾病的发生、发展及预后密切相关。文章通过总结tsRNA在妇科疾病中的研究进展,旨在为其机制研究、早期诊断和精准治疗提供新思路。
文摘转运RNA(transfer RNA,tRNA)衍生的小RNA(tRNA-derived small RNA,tsRNA)是一类新近发现的重要的非编码RNA分子。最近的研究表明,tsRNA在胃癌、结直肠癌等胃肠道肿瘤中表达水平发生显著变化。这种疾病相关的表达特征,使tsRNA有望成为这些疾病诊断和治疗的新型生物标志物。本文综述了tsRNA的来源、分类及其生物学功能,同时探讨tsRNA在胃癌、结直肠癌中的表达变化特点,为这些疾病的诊断和治疗提供了新思路。
基金supported by the National Natural Science Foundation of China(Nos.U24A20256,62472108,and 62472202)the Natural Science Foundation of Guangxi(No.2024GXNSFFA010006)+2 种基金the Hunan Intelligent Rehabilitation Robot and Auxiliary Equipment Engineering Technology Research Center(No.2025AQ104)the Guangxi Bagui Youth Talent Program,the Project of Guangxi Key Laboratory of Eye Health(No.GXYJK-202407)the Project of Guangxi Health Commission Eye and Related Diseases Artificial Intelligence Screen Technology Key Laboratory(No.GXYAI-202402).
文摘Increasing evidences have highlighted the significant association between tsRNAs and diseases.Predicting potential tsRNA-disease associations based on computational methods can effectively reduce human and resource consumption.However,there is a scarcity of computational methods for predicting tsRNA-disease associations.Therefore,we propose Contrastive Learning-based prediction of tsRNA-Disease Associations(CLTDA).It reconstructs known associations between tsRNAs and diseases based on adaptive Singular Value Decomposition(SVD).Then,we employ Graph Convolutional Networks(GCNs)for feature extraction from both the original and reconstructed tsRNA-disease associations,and optimize the GCNs by using contrastive learning loss and Bayesian Personalized Ranking(BPR)loss.In addition,the Bayesian negative sampling method is used to select high-quality negative samples for learning the features of tsRNA and disease.Finally,a Multi-Layer Perceptron(MLP)is utilized to calculates the score of potential association.We conduct five-fold cross-validation and denovo experiments on a manually collected tsRNA-disease association dataset,and the experimental results show that CLTDA outperforms the other six state-of-the-art methods.We also perform a case study on lung cancer and experimental results show that CLTDA is an effective tool for predicting potential associations between tsRNAs and diseases.
基金supported by National Natural Science Foundation of China(32121002,82330045,82125009)Shenzhen Medical Research Fund(B2502008)+6 种基金National Key R&D Program of China(2021YFA0804900)Major Natural Science Research Project of Anhui Province(2025AHGXZK20237)Changping Laboratory(2025B-07-13)Plans for Major Provincial Science&Technology Projects(202303a07020004)Major Frontier Research Project of the University of Science and Technology of China(LS9100000002)Hefei National Research Center for Physical Sciences at the Microscale(KF2025006)Hefei Comprehensive National Science Center Hefei Brain Project,Wannan Medical College Doctoral Research Initiation Fund(X600100192).
文摘Transfer RNA-derived small RNAs(tsRNAs),a class of non-coding RNAs derived from precursor or mature tRNAs,are now recognized as critical regulators in response to cellular stress.tsRNAs exhibit differential expression during brain aging and in age-related neurodegenerative disorders such as Alzheimer’s disease(AD),suggesting their involvement in the molecular processes underlying neuronal aging and degeneration.This article summarizes recent advances in our understanding of tsRNA biogenesis,classification,and function,emphasizing their regulatory role in brain aging and AD pathology.We also highlight the diagnostic and therapeutic implications of tsRNAs and discuss future directions for exploring their mechanistic and clinical relevance.
文摘越来越多的证据表明,上一代在环境压力下产生的某些获得性性状可以"记忆"在配子中,并以一种不依赖DNA序列的方式传递给下一代,这种现象被称为获得性遗传。然而,关于精子中介导获得性遗传现象的分子机制尚不清楚。近年来,随着精子RNA领域的发展,我们实验室率先发现精子中一类来源于成熟tRNA的小RNA (tRNA-derived small RNAs, tsRNAs)及其RNA修饰可以作为一种新型的表观遗传信息载体,介导父代获得性代谢紊乱性状向子代传递。此外,我们进一步发现了调控精子RNA介导获得性遗传的关键分子——tRNA甲基转移酶DNMT2,从RNA修饰及修饰酶的角度为获得性遗传的机制研究打开了新思路。鉴于该领域的迅速发展,该文拟从精子tsRNAs、RNA修饰以及DNMT2的角度综述近年来精子RNA及RNA修饰介导的获得性遗传机制的研究进展。
文摘为了解拟南芥中Dicer-like蛋白对tRNA衍生的小RNA(tRNA-derived small RNAs,tsRNAs)的产生有何影响,对拟南芥野生型和不同Dicer-like(DCL)基因突变体进行tRNA-seq测序,并分析tsRNA和tRNA的表达量.结果显示,DCL4基因突变后tsRNA的表达量明显降低,说明DCL4可能参与tsRNA的产生.拟南芥tRC1位点(Chr1:21268000-21310000)具有大量串联分布的tRNA序列,通过对RNA介导的甲基化(RdDM)途径相关基因CLSY1突变体中tRC1位点的24 nt siRNA和tsRNA进行分析,推断tRC1位点的tsRNA受RdDM途径负调控.综上,本研究鉴定到DCL4在tsRNA生成中的潜在作用,部分tsRNA的生成与RdDM途径有关.
