Objective The protein interacting with C kinase 1(PICK1)plays a critical role in vesicle trafficking,and its deficiency in sperm cells results in abnormal vesicle trafficking from Golgi to acrosome,which eventually di...Objective The protein interacting with C kinase 1(PICK1)plays a critical role in vesicle trafficking,and its deficiency in sperm cells results in abnormal vesicle trafficking from Golgi to acrosome,which eventually disrupts acrosome formation and leads to male infertility.Methods An azoospermia sample was filtered,and the laboratory detection and clinical phenotype indicated typical azoospermia in the patient.We sequenced all of the exons in the PICK1 gene and found that there was a novel homozygous variant in the PICK1 gene,c.364delA(p.Lys122SerfsX8),and this protein structure truncating variant seriously affected the biological function.Then we constructed a PICK1 knockout mouse model using clustered regularly interspaced short palindromic repeat cutting technology(CRISPRc).Results The sperm from PICK1 knockout mice showed acrosome and nucleus abnormalities,as well as dysfunctional mitochondrial sheath formation.Both the total sperm and motility sperm counts were decreased in the PICK1 knockout mice compared to wild-type mice.Moreover,the mitochondrial dysfunction was verified in the mice.These defects in the male PICK1 knockout mice may have eventually led to complete infertility.Conclusion The c.364delA novel variant in the PICK1 gene associated with clinical infertility,and pathogenic variants in the PICK1 may cause azoospermia or asthenospermia by impairing mitochondrial function in both mice and humans.展开更多
Gene therapy has shown promise for treating sensorineural hearing loss, supported by numerous successful preclinical studies. From the perspective of translation to humans, researchers have focused more on the genetic...Gene therapy has shown promise for treating sensorineural hearing loss, supported by numerous successful preclinical studies. From the perspective of translation to humans, researchers have focused more on the genetic causes of profound sensorineural hearing loss, where the sensory epithelium remains viable and intact for a considerable time after birth in humans. A key human deafness gene that best fits such a context is OTOF (GenBank AF183185.1), of which protein products, otoferlin, is essential for synaptic exocytosis and vesicle replenishment at the inner hair cell level in the cochlea.展开更多
The count of one column for high-dimensional datasets, i.e., the number of records containing this column, has been widely used in nuinerous applications such as analyzing popular spots based on check-in location info...The count of one column for high-dimensional datasets, i.e., the number of records containing this column, has been widely used in nuinerous applications such as analyzing popular spots based on check-in location information and mining valuable items from shopping records. However, this poses a privacy threat when directly publishing this information. Differential privacy (DP), as a notable paradigm for strong privacy guarantees, is thereby adopted to publish all column counts. Prior studies have verified that truncating records or grouping columns can effectively improve the accuracy of published results. To leverage the advantages of the two techniques, we combine these studies to further boost the accuracy of published results. However, the traditional penalty function, which measures the error imported by a given pair of parameters including truncating length and group size, is so sensitive that the derived parameters deviate from the optimal parameters significantly. To output preferable parameters, we first design a smart penalty function that is less sensitive than the traditional function. Moreover, a two-phase selection method is proposed to compute these parameters efficiently, together with the improvement in accuracy. Extensive experiments on a broad spectrum of real-world datasets validate the effectiveness of our proposals.展开更多
Mutations in the cyclin-dependent kinase-like 5 gene(CDKL5)cause a severe neurodevelopmental disorder,yet the impact of truncating mutations remains unclear.Here,we introduce the Cdkl5^(492stop) mouse model,mimicking ...Mutations in the cyclin-dependent kinase-like 5 gene(CDKL5)cause a severe neurodevelopmental disorder,yet the impact of truncating mutations remains unclear.Here,we introduce the Cdkl5^(492stop) mouse model,mimicking C-terminal truncating mutations in patients.492stop/Y mice exhibit altered dendritic spine morphology and spontaneous seizure-like behaviors,alongside other behavioral deficits.After creating cell lines with various Cdkl5 truncating mutations,we found that these mutations are regulated by the nonsense-mediated RNA decay pathway.Most truncating mutations result in CDKL5 protein loss,leading to multiple disease phenotypes,and offering new insights into the pathogenesis of CDKL5 disorder.展开更多
We consider the multiplicity of solutions to a p(x)-Laplacian problem involving supercritical Sobolev growth via Ricceri’s principle.By means of truncation combined with De Giorgi iteration,we can extend the results ...We consider the multiplicity of solutions to a p(x)-Laplacian problem involving supercritical Sobolev growth via Ricceri’s principle.By means of truncation combined with De Giorgi iteration,we can extend the results of subcritical and critical growth to supercritical growth and obtain at least three solutions to the p(x)-Laplacian problem.展开更多
In this paper,we introduce the real pairwise completely positive(RPCP)matrices with one of them is necessarily positive semidefinite while the other one is necessarily entrywise nonnegative,which has a real pairwise c...In this paper,we introduce the real pairwise completely positive(RPCP)matrices with one of them is necessarily positive semidefinite while the other one is necessarily entrywise nonnegative,which has a real pairwise completely positive(RPCP)decomposition.We study the properties of RPCP matrices and give some necessary and sufficient conditions for a matrix pair to be RPCP.First,we give an equivalent decomposition for the RPCP matrices,which is different from the RPCP-decomposition and show that the matrix pair(X,X)is RPCP if and only if X is completely positive.Besides,we also prove that the RPCP matrices checking problem is equivalent to the separable completion problem.A semidefinite algorithm is also proposed for detecting whether or not a matrix pair is RPCP.The asymptotic and finite convergence of the algorithm are also discussed.If it is RPCP,we can further give a RPCP-decomposition for it;if it is not,we can obtain a certificate for this.展开更多
A total of 393 potholes(368 fluvial and 25 marine potholes)were studied at seven different sites in both the fluvial and marine environments.Diverse bedrock properties and large-scale delivery of tools and grinders re...A total of 393 potholes(368 fluvial and 25 marine potholes)were studied at seven different sites in both the fluvial and marine environments.Diverse bedrock properties and large-scale delivery of tools and grinders regulate the dynamic growth,truncation,and amalgamation of potholes.Therefore,the principal objectives of the study are(i)to examine the relationship between the growth of potholes and substrate lithological with structural characteristics(applying geospatial and Schmidt hammer for rock strength analysis)and(ii)to measure the morphology,and size of tools and grinders,processes of truncation and amalgamation in hydro-geomorphic environment using various indices and field techniques.The result showed that large potholes are stretched in the direction of lineament axes and roughly parallel to the river flow direction.Here,the steady growth of pothole depth-diameter is controlled by active bedrock structures,tools,or grinders,and monsoonal high-velocity bank full discharge.Consequently,the deepening and widening of potholes are relatively slow at Bindu,Deuli,and marine beach Neil Island due to fewer structures and little supply of tools or grinders.In small stretches,(Damodar,Subarnarekha,and Rarhu)canyons and gorge-like features(bedrock incision)are formed at Rajrappa,Bhakuyadi,and Guridih sites due to cyclic truncation and amalgamation.Truncation and amalgamation processes restrict the vertical depth threshold value of potholes within 3m,particularly at Rajrappa,Bhakuyadi,and Guridih sites.Scientific study of the pothole's dynamic growth is greatly necessary for the different environmental engineering and river hydraulic projects like excavation,dredging,and dam or barrage construction.Successively,it is essential to compute the cost of rock excavation or dredging,primarily for the mechanical strength of the bedrock river channel and its stability.展开更多
While variable regions of immunoglobulins are extensively diversified by V(D)J recombination and somatic hypermutation in vertebrates,the constant regions of immunoglobulin heavy chains also utilize certain mechanisms...While variable regions of immunoglobulins are extensively diversified by V(D)J recombination and somatic hypermutation in vertebrates,the constant regions of immunoglobulin heavy chains also utilize certain mechanisms to produce diversity,including class switch recombination(CSR),subclass differentiation,and alternative expression of the same gene.Many species of birds,reptiles,and amphibians express a truncated isoform of immunoglobulin Y(IgY),termed IgY(ΔFc),which lacks theυCH3 andυCH4 domains.In Anseriformes,IgY(ΔFc)arises from alternative transcriptional termination sites within the sameυgene,whereas in some turtles,intact IgY and IgY(ΔFc)are encoded by distinct genes.Different from the previously reported IgY(ΔFc)variants,this study identified a truncated IgY in the snake Elaphe taeniura,characterized by the loss of only a portion of the CH4 domain.Western blotting and liquid chromatographytandem mass spectrometry confirmed that this truncated IgY is generated by post-translational cleavage at N338 within the IgY heavy chain constant(CH)region.Furthermore,both human and snake asparaginyl endopeptidase were shown to cleave snake IgY in vitro.These findings reveal a novel mechanism for the production of shortened IgY forms,demonstrating that the immunoglobulin CH region undergoes diversification through distinct strategies across vertebrates.展开更多
In industrial applications,plate-like structures such as steel strips in continuous hot-dip galvanizing and papers under fan action are ubiquitous.The vibration issues that arise when these structures are in axial mot...In industrial applications,plate-like structures such as steel strips in continuous hot-dip galvanizing and papers under fan action are ubiquitous.The vibration issues that arise when these structures are in axial motion,and are influenced by fluids and thermal fields,have attracted significant attention from the academic community.This study focuses on the nonlinear dynamic behavior of axially transporting immersed viscoelastic plates with particular emphasis on internal resonance and speed-dependent tension.The governing equation and the related boundary conditions for the axially transporting viscoelastic immersed plate are derived with Hamilton's principle,prioritizing the impact of time-varying tension induced by speed perturbations.Based on the second-order Galerkin truncation,the governing equation is discretized into a system of second-order ordinary differential equations.The multi-scale method is used to analyze the stable steady-state response of the immersed viscoelastic plate.The conditions for achieving a 3:1 frequency ratio between the first two orders of the system are analytically deduced.Notably,when the viscoelastic coefficient diminishes,the stability boundaries exhibit increased complexity,manifesting as the irregular W-shaped contours in the parameter space.Numerical examples comprehensively investigate the effects of viscoelasticity on both the stability region and the steady-state response under internal resonance conditions.Finally,the accuracy of the obtained results is validated through numerical computation.展开更多
The selection of the truncation level （TL） and the control of boundary effect （BE） are critical in regional geomagnetic field models that are based on data fitting. We combine Taylor and Legendre polynomials to mo...The selection of the truncation level （TL） and the control of boundary effect （BE） are critical in regional geomagnetic field models that are based on data fitting. We combine Taylor and Legendre polynomials to model geomagnetic data over China's Mainland for years 1960, 1970, 1990, and 2000. To tackle the TL and BE problems, we first determine the range of TL by calculating the root-mean-square error （RMSE） of the models. Next, we determine the optimum TL using the Akaike information criterion （AIC） and the normalized root- mean-square error （NRMSE）. We use the regional anomaly addition （RAA） and the uniform addition （UA） method to add supplementary point outside the national boundary, and find that the intensities of extreme points gradually decrease and stabilize. The UA method better controls BEs over China, whereas the RAA method does a better job at smaller scales. In summary, we rely on a three-step method to determine the optimum TL and propose criteria to determine the optimum number of supplementary points.展开更多
This paper presents an explicit difference scheme with accuracy and branching stability for solving onedimensional parabolic type equation by the method of undetermined parameters and its truncation error is O(△t4+△...This paper presents an explicit difference scheme with accuracy and branching stability for solving onedimensional parabolic type equation by the method of undetermined parameters and its truncation error is O(△t4+△x4). The stability condition is r=a△t/△x2<1/2.展开更多
The situation of occurrence and hazards by Tetranychus truncatus and T. urticae on corns in test garden of Agricultural College of Yanbian University were narrated in detail. The characteristics of distribution and da...The situation of occurrence and hazards by Tetranychus truncatus and T. urticae on corns in test garden of Agricultural College of Yanbian University were narrated in detail. The characteristics of distribution and damage by T. truncates and T. urticae, morphological characteristics, the rule of currencies and its relation- ship with environment were summarized, and the methods of comprehensive treat- ment were put out, which could effectively control the hazard degree of T. truncates and T. urticae on corns.展开更多
The research, based on pol ens of Zygocactus truncates during ful-bloom period, selected suitable culture medium and temperature for pol en germination and explored the effects of storage temperatures and pol en col e...The research, based on pol ens of Zygocactus truncates during ful-bloom period, selected suitable culture medium and temperature for pol en germination and explored the effects of storage temperatures and pol en col ection methods on pol en vitality of Zygocactus truncates and the results showed that the culture medium containing 200 g/L saccharose, 20 mg/L boric acid, and 20 mg/L calcium chloride is suitable for pol en germination of Zygocactus truncates; when the temperature kept below 20 ℃, pol en germination period last short, and germination rate kept higher;pol en germination performed better with filaments, and pol en tube grew wel .展开更多
In this paper we propose an absolute error loss EB estimator for parameter of one-side truncation distribution families. Under some conditions we have proved that the convergence rates of its Bayes risk is o, where 0&...In this paper we propose an absolute error loss EB estimator for parameter of one-side truncation distribution families. Under some conditions we have proved that the convergence rates of its Bayes risk is o, where 0<λ,r≤1,Mn≤lnln n (for large n),Mn→∞ as n→∞.展开更多
为了解决室内动态环境下移动机器人的准确定位问题,提出了一种融合运动检测算法的半直接法RGB-D视觉SLAM(同时定位与地图创建)算法,它由运动检测、相机位姿估计、基于TSDF (truncated signed distance function)模型的稠密地图构建3个...为了解决室内动态环境下移动机器人的准确定位问题,提出了一种融合运动检测算法的半直接法RGB-D视觉SLAM(同时定位与地图创建)算法,它由运动检测、相机位姿估计、基于TSDF (truncated signed distance function)模型的稠密地图构建3个步骤组成.首先,通过最小化图像光度误差,利用稀疏图像对齐算法实现对相机位姿的初步估计.然后,使用视觉里程计的位姿估计对图像进行运动补偿,建立基于图像块实时更新的高斯模型,依据方差变化分割出图像中的运动物体,进而剔除投影在图像运动区域的局部地图点,通过最小化重投影误差对相机位姿进行进一步优化,提升相机位姿估计精度.最后,使用相机位姿和RGB-D相机图像信息构建TSDF稠密地图,利用图像运动检测结果和地图体素块的颜色变化,完成地图在动态环境下的实时更新.实验结果表明,在室内动态环境下,本文算法能够有效提高相机位姿估计精度,实现稠密地图的实时更新,在提升系统鲁棒性的同时也提升了环境重构的准确性.展开更多
In this paper, first we calculate finite-difference coefficients of implicit finite- difference methods （IFDM） for the first and second-order derivatives on normal grids and first- order derivatives on staggered gri...In this paper, first we calculate finite-difference coefficients of implicit finite- difference methods （IFDM） for the first and second-order derivatives on normal grids and first- order derivatives on staggered grids and find that small coefficients of high-order IFDMs exist. Dispersion analysis demonstrates that omitting these small coefficients can retain approximately the same order accuracy but greatly reduce computational costs. Then, we introduce a mirrorimage symmetric boundary condition to improve IFDMs accuracy and stability and adopt the hybrid absorbing boundary condition （ABC） to reduce unwanted reflections from the model boundary. Last, we give elastic wave modeling examples for homogeneous and heterogeneous models to demonstrate the advantages of the proposed scheme.展开更多
In order to decrease the probability of missing some data points or noises being added in the inverse truncated mixing matrix （ITMM） algorithm, a two-stage frequency- domain method is proposed for blind source separ...In order to decrease the probability of missing some data points or noises being added in the inverse truncated mixing matrix （ITMM） algorithm, a two-stage frequency- domain method is proposed for blind source separation of underdetermined instantaneous mixtures. The separation process is decomposed into two steps of ITMM and matrix completion in the view that there are many soft-sparse （not very sparse） sources. First, the mixing matrix is estimated and the sources are recovered by the traditional ITMM algorithm in the frequency domain. Then, in order to retrieve the missing data and remove noises, the matrix completion technique is applied to each preliminary estimated source by the traditional ITMM algorithm in the frequency domain. Simulations show that, compared with the traditional ITMM algorithms, the proposed two-stage algorithm has better separation performances. In addition, the time consumption problem is considered. The proposed algorithm outperforms the traditional ITMM algorithm at a cost of no more than one- fourth extra time consumption.展开更多
基金supported by grants from Zhejiang Provincial Natural Science Foundation of China(No.LQ21H200007)National Natural Science Foundation of China(No.82202605,No.81772664 and No.82172363)+1 种基金Zhejiang Provincial People’s Hospital Excellent Scientific Research Start-up Fundation of China(No.ZRY2019C008)Hangzhou Medical College Fundamental Scientific Research Project of China(No.KYQN202116).
文摘Objective The protein interacting with C kinase 1(PICK1)plays a critical role in vesicle trafficking,and its deficiency in sperm cells results in abnormal vesicle trafficking from Golgi to acrosome,which eventually disrupts acrosome formation and leads to male infertility.Methods An azoospermia sample was filtered,and the laboratory detection and clinical phenotype indicated typical azoospermia in the patient.We sequenced all of the exons in the PICK1 gene and found that there was a novel homozygous variant in the PICK1 gene,c.364delA(p.Lys122SerfsX8),and this protein structure truncating variant seriously affected the biological function.Then we constructed a PICK1 knockout mouse model using clustered regularly interspaced short palindromic repeat cutting technology(CRISPRc).Results The sperm from PICK1 knockout mice showed acrosome and nucleus abnormalities,as well as dysfunctional mitochondrial sheath formation.Both the total sperm and motility sperm counts were decreased in the PICK1 knockout mice compared to wild-type mice.Moreover,the mitochondrial dysfunction was verified in the mice.These defects in the male PICK1 knockout mice may have eventually led to complete infertility.Conclusion The c.364delA novel variant in the PICK1 gene associated with clinical infertility,and pathogenic variants in the PICK1 may cause azoospermia or asthenospermia by impairing mitochondrial function in both mice and humans.
基金supported by the Basic Science Research Program through the National Research Foundation of Korea,funded by the Ministry of Education,South Korea(No.2021R1A2C2092038 to B.Y.C.,2022R1I1A3072036 to E.Y.)Bio Core Facility center program through the NRF(No.2022M3A9G1014007 to B.Y.C.)+2 种基金also by the Basic Research Laboratory program through the NRF,funded by the Ministry of Science&ICT(MSIT)(No.RS-2023-0021971031482092640001 to B.Y.C.)the Technology Innovation Program(No.K_G012002572001 to B.Y.C.)funded by the Korean Ministry of TradeIndustry and Energy.This study is also funded by the Seoul National University Bundang Hospital intramural research fund(No.16-2020-0009,16-2022-0005,13-2023-0002,16-2023-0002,18-2023-0004,and 13-2024-0004 to B.Y.C.).
文摘Gene therapy has shown promise for treating sensorineural hearing loss, supported by numerous successful preclinical studies. From the perspective of translation to humans, researchers have focused more on the genetic causes of profound sensorineural hearing loss, where the sensory epithelium remains viable and intact for a considerable time after birth in humans. A key human deafness gene that best fits such a context is OTOF (GenBank AF183185.1), of which protein products, otoferlin, is essential for synaptic exocytosis and vesicle replenishment at the inner hair cell level in the cochlea.
基金the National Natural Science Foundation of China (Grant Nos. 61433008, 61472071 and U143520006)the Fundamental Research Funds for the Central Universities of China (161604005 and 171605001)the Natural Science Foundation of Liaoning Province (2015020018).
文摘The count of one column for high-dimensional datasets, i.e., the number of records containing this column, has been widely used in nuinerous applications such as analyzing popular spots based on check-in location information and mining valuable items from shopping records. However, this poses a privacy threat when directly publishing this information. Differential privacy (DP), as a notable paradigm for strong privacy guarantees, is thereby adopted to publish all column counts. Prior studies have verified that truncating records or grouping columns can effectively improve the accuracy of published results. To leverage the advantages of the two techniques, we combine these studies to further boost the accuracy of published results. However, the traditional penalty function, which measures the error imported by a given pair of parameters including truncating length and group size, is so sensitive that the derived parameters deviate from the optimal parameters significantly. To output preferable parameters, we first design a smart penalty function that is less sensitive than the traditional function. Moreover, a two-phase selection method is proposed to compute these parameters efficiently, together with the improvement in accuracy. Extensive experiments on a broad spectrum of real-world datasets validate the effectiveness of our proposals.
基金supported by the Innovation of Science and Technology 2030-Major Project"Platform of Nonhuman Primate Models"(2021ZD0200900)the Ministry of Science and Technology(2018YFA0801404)+1 种基金the National Natural Science Foundation of China(82021001)the Shanghai Municipal Science and Technology Major Project.
文摘Mutations in the cyclin-dependent kinase-like 5 gene(CDKL5)cause a severe neurodevelopmental disorder,yet the impact of truncating mutations remains unclear.Here,we introduce the Cdkl5^(492stop) mouse model,mimicking C-terminal truncating mutations in patients.492stop/Y mice exhibit altered dendritic spine morphology and spontaneous seizure-like behaviors,alongside other behavioral deficits.After creating cell lines with various Cdkl5 truncating mutations,we found that these mutations are regulated by the nonsense-mediated RNA decay pathway.Most truncating mutations result in CDKL5 protein loss,leading to multiple disease phenotypes,and offering new insights into the pathogenesis of CDKL5 disorder.
基金supported by the Fundamental Research Funds for the Central Universities(2024KYJD2006).
文摘We consider the multiplicity of solutions to a p(x)-Laplacian problem involving supercritical Sobolev growth via Ricceri’s principle.By means of truncation combined with De Giorgi iteration,we can extend the results of subcritical and critical growth to supercritical growth and obtain at least three solutions to the p(x)-Laplacian problem.
文摘In this paper,we introduce the real pairwise completely positive(RPCP)matrices with one of them is necessarily positive semidefinite while the other one is necessarily entrywise nonnegative,which has a real pairwise completely positive(RPCP)decomposition.We study the properties of RPCP matrices and give some necessary and sufficient conditions for a matrix pair to be RPCP.First,we give an equivalent decomposition for the RPCP matrices,which is different from the RPCP-decomposition and show that the matrix pair(X,X)is RPCP if and only if X is completely positive.Besides,we also prove that the RPCP matrices checking problem is equivalent to the separable completion problem.A semidefinite algorithm is also proposed for detecting whether or not a matrix pair is RPCP.The asymptotic and finite convergence of the algorithm are also discussed.If it is RPCP,we can further give a RPCP-decomposition for it;if it is not,we can obtain a certificate for this.
文摘A total of 393 potholes(368 fluvial and 25 marine potholes)were studied at seven different sites in both the fluvial and marine environments.Diverse bedrock properties and large-scale delivery of tools and grinders regulate the dynamic growth,truncation,and amalgamation of potholes.Therefore,the principal objectives of the study are(i)to examine the relationship between the growth of potholes and substrate lithological with structural characteristics(applying geospatial and Schmidt hammer for rock strength analysis)and(ii)to measure the morphology,and size of tools and grinders,processes of truncation and amalgamation in hydro-geomorphic environment using various indices and field techniques.The result showed that large potholes are stretched in the direction of lineament axes and roughly parallel to the river flow direction.Here,the steady growth of pothole depth-diameter is controlled by active bedrock structures,tools,or grinders,and monsoonal high-velocity bank full discharge.Consequently,the deepening and widening of potholes are relatively slow at Bindu,Deuli,and marine beach Neil Island due to fewer structures and little supply of tools or grinders.In small stretches,(Damodar,Subarnarekha,and Rarhu)canyons and gorge-like features(bedrock incision)are formed at Rajrappa,Bhakuyadi,and Guridih sites due to cyclic truncation and amalgamation.Truncation and amalgamation processes restrict the vertical depth threshold value of potholes within 3m,particularly at Rajrappa,Bhakuyadi,and Guridih sites.Scientific study of the pothole's dynamic growth is greatly necessary for the different environmental engineering and river hydraulic projects like excavation,dredging,and dam or barrage construction.Successively,it is essential to compute the cost of rock excavation or dredging,primarily for the mechanical strength of the bedrock river channel and its stability.
基金supported by the Fundamental Research Funds for the Central Universities,Southwest Minzu University(ZYN2023097)Scientific and Technological Innovation Team for Qinghai-Xizang Plateau Research in Southwest Minzu University(2024CXTD13)。
文摘While variable regions of immunoglobulins are extensively diversified by V(D)J recombination and somatic hypermutation in vertebrates,the constant regions of immunoglobulin heavy chains also utilize certain mechanisms to produce diversity,including class switch recombination(CSR),subclass differentiation,and alternative expression of the same gene.Many species of birds,reptiles,and amphibians express a truncated isoform of immunoglobulin Y(IgY),termed IgY(ΔFc),which lacks theυCH3 andυCH4 domains.In Anseriformes,IgY(ΔFc)arises from alternative transcriptional termination sites within the sameυgene,whereas in some turtles,intact IgY and IgY(ΔFc)are encoded by distinct genes.Different from the previously reported IgY(ΔFc)variants,this study identified a truncated IgY in the snake Elaphe taeniura,characterized by the loss of only a portion of the CH4 domain.Western blotting and liquid chromatographytandem mass spectrometry confirmed that this truncated IgY is generated by post-translational cleavage at N338 within the IgY heavy chain constant(CH)region.Furthermore,both human and snake asparaginyl endopeptidase were shown to cleave snake IgY in vitro.These findings reveal a novel mechanism for the production of shortened IgY forms,demonstrating that the immunoglobulin CH region undergoes diversification through distinct strategies across vertebrates.
基金Project supported by the Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China(No.2023KJ215)the National Natural Science Foundation of China(Nos.12002142 and 52405274)the National Natural Science Foundation of Shanghai of China(No.ZR2023QE100)。
文摘In industrial applications,plate-like structures such as steel strips in continuous hot-dip galvanizing and papers under fan action are ubiquitous.The vibration issues that arise when these structures are in axial motion,and are influenced by fluids and thermal fields,have attracted significant attention from the academic community.This study focuses on the nonlinear dynamic behavior of axially transporting immersed viscoelastic plates with particular emphasis on internal resonance and speed-dependent tension.The governing equation and the related boundary conditions for the axially transporting viscoelastic immersed plate are derived with Hamilton's principle,prioritizing the impact of time-varying tension induced by speed perturbations.Based on the second-order Galerkin truncation,the governing equation is discretized into a system of second-order ordinary differential equations.The multi-scale method is used to analyze the stable steady-state response of the immersed viscoelastic plate.The conditions for achieving a 3:1 frequency ratio between the first two orders of the system are analytically deduced.Notably,when the viscoelastic coefficient diminishes,the stability boundaries exhibit increased complexity,manifesting as the irregular W-shaped contours in the parameter space.Numerical examples comprehensively investigate the effects of viscoelasticity on both the stability region and the steady-state response under internal resonance conditions.Finally,the accuracy of the obtained results is validated through numerical computation.
基金supported by the National Natural Science Foundation of China(Grant Nos.41404053 and 41174165)Special Project for Meteo-Scientific Research in the Public Interest(Grant No.GYHY201306073)Natural Science Foundation of Higher Education Institutions of Jiangsu Province,China(Grant No.14KJB170012)
文摘The selection of the truncation level （TL） and the control of boundary effect （BE） are critical in regional geomagnetic field models that are based on data fitting. We combine Taylor and Legendre polynomials to model geomagnetic data over China's Mainland for years 1960, 1970, 1990, and 2000. To tackle the TL and BE problems, we first determine the range of TL by calculating the root-mean-square error （RMSE） of the models. Next, we determine the optimum TL using the Akaike information criterion （AIC） and the normalized root- mean-square error （NRMSE）. We use the regional anomaly addition （RAA） and the uniform addition （UA） method to add supplementary point outside the national boundary, and find that the intensities of extreme points gradually decrease and stabilize. The UA method better controls BEs over China, whereas the RAA method does a better job at smaller scales. In summary, we rely on a three-step method to determine the optimum TL and propose criteria to determine the optimum number of supplementary points.
文摘This paper presents an explicit difference scheme with accuracy and branching stability for solving onedimensional parabolic type equation by the method of undetermined parameters and its truncation error is O(△t4+△x4). The stability condition is r=a△t/△x2<1/2.
文摘The situation of occurrence and hazards by Tetranychus truncatus and T. urticae on corns in test garden of Agricultural College of Yanbian University were narrated in detail. The characteristics of distribution and damage by T. truncates and T. urticae, morphological characteristics, the rule of currencies and its relation- ship with environment were summarized, and the methods of comprehensive treat- ment were put out, which could effectively control the hazard degree of T. truncates and T. urticae on corns.
文摘The research, based on pol ens of Zygocactus truncates during ful-bloom period, selected suitable culture medium and temperature for pol en germination and explored the effects of storage temperatures and pol en col ection methods on pol en vitality of Zygocactus truncates and the results showed that the culture medium containing 200 g/L saccharose, 20 mg/L boric acid, and 20 mg/L calcium chloride is suitable for pol en germination of Zygocactus truncates; when the temperature kept below 20 ℃, pol en germination period last short, and germination rate kept higher;pol en germination performed better with filaments, and pol en tube grew wel .
文摘In this paper we propose an absolute error loss EB estimator for parameter of one-side truncation distribution families. Under some conditions we have proved that the convergence rates of its Bayes risk is o, where 0<λ,r≤1,Mn≤lnln n (for large n),Mn→∞ as n→∞.
文摘为了解决室内动态环境下移动机器人的准确定位问题,提出了一种融合运动检测算法的半直接法RGB-D视觉SLAM(同时定位与地图创建)算法,它由运动检测、相机位姿估计、基于TSDF (truncated signed distance function)模型的稠密地图构建3个步骤组成.首先,通过最小化图像光度误差,利用稀疏图像对齐算法实现对相机位姿的初步估计.然后,使用视觉里程计的位姿估计对图像进行运动补偿,建立基于图像块实时更新的高斯模型,依据方差变化分割出图像中的运动物体,进而剔除投影在图像运动区域的局部地图点,通过最小化重投影误差对相机位姿进行进一步优化,提升相机位姿估计精度.最后,使用相机位姿和RGB-D相机图像信息构建TSDF稠密地图,利用图像运动检测结果和地图体素块的颜色变化,完成地图在动态环境下的实时更新.实验结果表明,在室内动态环境下,本文算法能够有效提高相机位姿估计精度,实现稠密地图的实时更新,在提升系统鲁棒性的同时也提升了环境重构的准确性.
基金supported by the National Natural Science Foundation of China(NSFC)(Grant No. 41074100)the Program for New Century Excellent Talents in University of Ministry of Education of China(Grant No. NCET-10-0812)
文摘In this paper, first we calculate finite-difference coefficients of implicit finite- difference methods （IFDM） for the first and second-order derivatives on normal grids and first- order derivatives on staggered grids and find that small coefficients of high-order IFDMs exist. Dispersion analysis demonstrates that omitting these small coefficients can retain approximately the same order accuracy but greatly reduce computational costs. Then, we introduce a mirrorimage symmetric boundary condition to improve IFDMs accuracy and stability and adopt the hybrid absorbing boundary condition （ABC） to reduce unwanted reflections from the model boundary. Last, we give elastic wave modeling examples for homogeneous and heterogeneous models to demonstrate the advantages of the proposed scheme.
基金The National Natural Science Foundation of China(No.60872074)
文摘In order to decrease the probability of missing some data points or noises being added in the inverse truncated mixing matrix （ITMM） algorithm, a two-stage frequency- domain method is proposed for blind source separation of underdetermined instantaneous mixtures. The separation process is decomposed into two steps of ITMM and matrix completion in the view that there are many soft-sparse （not very sparse） sources. First, the mixing matrix is estimated and the sources are recovered by the traditional ITMM algorithm in the frequency domain. Then, in order to retrieve the missing data and remove noises, the matrix completion technique is applied to each preliminary estimated source by the traditional ITMM algorithm in the frequency domain. Simulations show that, compared with the traditional ITMM algorithms, the proposed two-stage algorithm has better separation performances. In addition, the time consumption problem is considered. The proposed algorithm outperforms the traditional ITMM algorithm at a cost of no more than one- fourth extra time consumption.
