Rice blast is a devastating disease worldwide,threatening rice production and food security.The blast fun-gus Magnaporthe oryzae invades the host via the appressorium,a specialized pressure-generating struc-ture that ...Rice blast is a devastating disease worldwide,threatening rice production and food security.The blast fun-gus Magnaporthe oryzae invades the host via the appressorium,a specialized pressure-generating struc-ture that generates enormous turgor pressure to penetrate the host cuticle.However,owing to ongoing evolution of fungicide resistance,it is vitally important to identify new targets and fungicides.Here,we show that Trs85,a subunit of the transport protein particle III complex,is essential for appressorium-mediated infection in M.oryzae.We explain how Trs85 regulates autophagy through Ypt1(a small guano-sine triphosphatase protein)in M.oryzae.We then identify a key conserved amphipathic a helix within Trs85 that is associated with pathogenicity of M.oryzae.Through computer-aided screening,we identify a lead compound,SP-141,that affects autophagy and the Trs85–Ypt1 interaction.SP-141 demonstrates a substantial capacity to effectively inhibit infection caused by the rice blast fungus while also exhibiting wide-ranging potential as an antifungal agent with broad-spectrum activity.Taken together,our data show that Trs85 is a potential new target and that SP-141 has potential for the control of rice blast.Ourfindings thus provide a novel strategy that may help in thefight against rice blast.展开更多
基金supported by the Key Research and Development Project of Zhejiang Province,China (2021C02010)the National Natural Science Foundation of China (32270201,31972216,and 31970140)+1 种基金the Special Project for the Selection and Breeding of New Agricultural Varieties in Zhejiang Province,China (2021C02064)supported by a grant,Organism Interaction,from Zhejiang Xianghu Laboratory (to F.-C.L.).
文摘Rice blast is a devastating disease worldwide,threatening rice production and food security.The blast fun-gus Magnaporthe oryzae invades the host via the appressorium,a specialized pressure-generating struc-ture that generates enormous turgor pressure to penetrate the host cuticle.However,owing to ongoing evolution of fungicide resistance,it is vitally important to identify new targets and fungicides.Here,we show that Trs85,a subunit of the transport protein particle III complex,is essential for appressorium-mediated infection in M.oryzae.We explain how Trs85 regulates autophagy through Ypt1(a small guano-sine triphosphatase protein)in M.oryzae.We then identify a key conserved amphipathic a helix within Trs85 that is associated with pathogenicity of M.oryzae.Through computer-aided screening,we identify a lead compound,SP-141,that affects autophagy and the Trs85–Ypt1 interaction.SP-141 demonstrates a substantial capacity to effectively inhibit infection caused by the rice blast fungus while also exhibiting wide-ranging potential as an antifungal agent with broad-spectrum activity.Taken together,our data show that Trs85 is a potential new target and that SP-141 has potential for the control of rice blast.Ourfindings thus provide a novel strategy that may help in thefight against rice blast.
