Several sequences were applied to resolve phylogenetic relationships within Dendranthema and clarify the origin of garden chrysanthemums including sequences of nrDNA ITS, trnT-trnL and trnL-trnF intergenic spacer regi...Several sequences were applied to resolve phylogenetic relationships within Dendranthema and clarify the origin of garden chrysanthemums including sequences of nrDNA ITS, trnT-trnL and trnL-trnF intergenic spacer regions in cpDNA. The relationships among the species are so close that the three sequences could only provide limited information. From the evidence presented, we suggest that: ① D.rhmobifolium be the chloroplast donor of D.vestitum (HN) with the resembling morphology and the same trnT/L IGS sequence. ② D.vestitum, a putative ancestor, may be not the chloroplast donor of garden chrysanthemums. D.lavand-ulifolium might be the chloroplast donor of the type population of D.indicum (HN) or the direct chloroplast donor of the ancient garden chrysanthemum cultivar. ③ D.zawaskii might be not the ancestor of garden chrysanthemums.展开更多
目的:分析比较石蒜属和近缘属植物中国水仙trnL基因的部分序列和trnL-trnF间隔区的完整序列,为石蒜属物种鉴别和种间亲缘关系分析提供分子佐证。方法:采用PCR产物直接双向测序法,用Clustal X 1.81,MEGA 2.0软件进行序列分析,采用最大简...目的:分析比较石蒜属和近缘属植物中国水仙trnL基因的部分序列和trnL-trnF间隔区的完整序列,为石蒜属物种鉴别和种间亲缘关系分析提供分子佐证。方法:采用PCR产物直接双向测序法,用Clustal X 1.81,MEGA 2.0软件进行序列分析,采用最大简约法(maximum parsimony,MP)构建系统发育树。结果:所测物种的序列全长在905～1036 bp,经排序后,两端切平,得到886 bp的整齐序列,所测物种的核苷酸变异位点14个,信息位点10个,主要在trnL内含子区,这些位点可用于区分石蒜属物种;4个碱基"ATAT"缺失/插入是区别石蒜属与其近缘植物水仙的显著特征。重建的系统发育树表明:供试的石蒜属物种聚成3类,除稻草石蒜、忽地笑、香石蒜的系统位置有所不同之外,与经典分类结果基本相符。水仙属2个种形成1个单系类群,表明属间trnL-F序列差异明显。结论:分子系统树支持安徽石蒜是长筒石蒜的变种或杂交种观点,换锦花可能是玫瑰石蒜、红蓝石蒜的杂交母本。石蒜属种间trnL-F序列存在一定变异,是物种鉴别的有效分子标记。展开更多
文摘Several sequences were applied to resolve phylogenetic relationships within Dendranthema and clarify the origin of garden chrysanthemums including sequences of nrDNA ITS, trnT-trnL and trnL-trnF intergenic spacer regions in cpDNA. The relationships among the species are so close that the three sequences could only provide limited information. From the evidence presented, we suggest that: ① D.rhmobifolium be the chloroplast donor of D.vestitum (HN) with the resembling morphology and the same trnT/L IGS sequence. ② D.vestitum, a putative ancestor, may be not the chloroplast donor of garden chrysanthemums. D.lavand-ulifolium might be the chloroplast donor of the type population of D.indicum (HN) or the direct chloroplast donor of the ancient garden chrysanthemum cultivar. ③ D.zawaskii might be not the ancestor of garden chrysanthemums.
