A triple helix composed of three dodecanucleotides,5'-d(TC)6,5-(AG)6 and 3'-d(TC)6 with each containing a methylcytosme or a protonated-cylosine,was examined in terms of various interactions Computational malv...A triple helix composed of three dodecanucleotides,5'-d(TC)6,5-(AG)6 and 3'-d(TC)6 with each containing a methylcytosme or a protonated-cylosine,was examined in terms of various interactions Computational malvsis of the stability of triplex formation was consistent with the experimental observations.The Hoogsleen hase among interaction was increased through substitution of 5-methylcytosme for cytosme and/or protonation The inter-woon between water molecules and DNA triplex was visualized in terms,of the end-to-end distance change of each trand The chain length was reduced by about 7 5%-16 2% due to the screening effect In addition,as the screening fleer nerenises,Hoogsteen base pairs appear to be stable in triplex formation.Our computational results also show this the effect of protonated- and methylated cytosme on the stability of triplex formation was due to the change of the beerostanis mteractions rather than the surface stacking interaction展开更多
基金Work supported by the Chinese Academy of Sciences
文摘A triple helix composed of three dodecanucleotides,5'-d(TC)6,5-(AG)6 and 3'-d(TC)6 with each containing a methylcytosme or a protonated-cylosine,was examined in terms of various interactions Computational malvsis of the stability of triplex formation was consistent with the experimental observations.The Hoogsleen hase among interaction was increased through substitution of 5-methylcytosme for cytosme and/or protonation The inter-woon between water molecules and DNA triplex was visualized in terms,of the end-to-end distance change of each trand The chain length was reduced by about 7 5%-16 2% due to the screening effect In addition,as the screening fleer nerenises,Hoogsteen base pairs appear to be stable in triplex formation.Our computational results also show this the effect of protonated- and methylated cytosme on the stability of triplex formation was due to the change of the beerostanis mteractions rather than the surface stacking interaction
