Heptoses are important structural components of Gram-negative bacterium cell wall and participate in bacterial colonization,infection,and immune recognition.Current knowledge of NDP-heptose originating from D-sedohept...Heptoses are important structural components of Gram-negative bacterium cell wall and participate in bacterial colonization,infection,and immune recognition.Current knowledge of NDP-heptose originating from D-sedoheptulose 7-phosphate in Grampositive bacterium remains limited.Here,in silico analysis suggested that the special tridomain NDP-heptose synthetases with isomerase,kinase,and nucleotidyltransferase activities are conservatively distributed in Actinobacteria class of Gram-positive bacterium.Enzymatical characterization of the tridomain proteins from different strains showed that they are involved in ADPD-glycero-β-D-manno-heptose biosynthesis despite the unexpected discovery of kinase activities deficient in some proteins.The presence of three types of NDP-heptose synthetases in Gram-positive bacterium suggests that it is also a rich source of heptoses and the heptose moieties may play important roles in vivo.Our work updates the understanding of NDP-heptose biosynthesis in Gram-positive bacterium and lays a solid foundation for further physiological function explorations.展开更多
目的 三结构域蛋白44(TRIM44)在喉鳞状细胞癌(LSCC)中的表达水平及其与预后的相关研究。方法 选取2018年10月至2019年10月自贡市第一人民医院收治的98例LSCC患者,术中分别取其LSCC组织和癌旁组织,采用q RT-PCR法检测TRIM44 m RNA在LSCC...目的 三结构域蛋白44(TRIM44)在喉鳞状细胞癌(LSCC)中的表达水平及其与预后的相关研究。方法 选取2018年10月至2019年10月自贡市第一人民医院收治的98例LSCC患者,术中分别取其LSCC组织和癌旁组织,采用q RT-PCR法检测TRIM44 m RNA在LSCC组织、癌旁组织中的表达水平;根据LSCC组织TRIM44 m RNA表达量的平均值分为TRIM44低表达组和TRIM44高表达组;分析TRIM44 m RNA表达水平与LSCC患者生存状况的关系;分析影响LSCC患者预后不良的危险因素。结果 与癌旁组织相比,LSCC组织TRIM44阳性表达率显著升高(P<0.05)。与癌旁组织相比,LSCC组织TRIM44m RNA的表达水平显著升高(P<0.05);TRIM44 m RNA表达水平与淋巴结转移、分化程度、远处转移有关(P<0.05)。分析显示,TRIM44高表达组患者3年生存率低于TRIM44低表达组(P<0.05);生存患者与死亡患者在肿瘤分化程度(低分化)、淋巴结转移及远处转移比例方面存在显著差异。(P<0.05)。淋巴结转移(HR=3.332)、TRIM44 m RNA高表达(HR=5.365)是影响LSCC患者预后不良的危险因素(P<0.05)。结论 TRIM44 m RNA在LSCC组织中呈高表达,与LSCC患者临床特征及生存情况有关,有望成为潜在的LSCC预后生物标志物。展开更多
基金supported in part by the National Key R&D Program of China(2020YFA0907700)the National Natural Science Foundation of China(32025002)the Center for Ocean Mega-Science,CAS(KEXUE2019GZ05)。
文摘Heptoses are important structural components of Gram-negative bacterium cell wall and participate in bacterial colonization,infection,and immune recognition.Current knowledge of NDP-heptose originating from D-sedoheptulose 7-phosphate in Grampositive bacterium remains limited.Here,in silico analysis suggested that the special tridomain NDP-heptose synthetases with isomerase,kinase,and nucleotidyltransferase activities are conservatively distributed in Actinobacteria class of Gram-positive bacterium.Enzymatical characterization of the tridomain proteins from different strains showed that they are involved in ADPD-glycero-β-D-manno-heptose biosynthesis despite the unexpected discovery of kinase activities deficient in some proteins.The presence of three types of NDP-heptose synthetases in Gram-positive bacterium suggests that it is also a rich source of heptoses and the heptose moieties may play important roles in vivo.Our work updates the understanding of NDP-heptose biosynthesis in Gram-positive bacterium and lays a solid foundation for further physiological function explorations.
文摘目的 三结构域蛋白44(TRIM44)在喉鳞状细胞癌(LSCC)中的表达水平及其与预后的相关研究。方法 选取2018年10月至2019年10月自贡市第一人民医院收治的98例LSCC患者,术中分别取其LSCC组织和癌旁组织,采用q RT-PCR法检测TRIM44 m RNA在LSCC组织、癌旁组织中的表达水平;根据LSCC组织TRIM44 m RNA表达量的平均值分为TRIM44低表达组和TRIM44高表达组;分析TRIM44 m RNA表达水平与LSCC患者生存状况的关系;分析影响LSCC患者预后不良的危险因素。结果 与癌旁组织相比,LSCC组织TRIM44阳性表达率显著升高(P<0.05)。与癌旁组织相比,LSCC组织TRIM44m RNA的表达水平显著升高(P<0.05);TRIM44 m RNA表达水平与淋巴结转移、分化程度、远处转移有关(P<0.05)。分析显示,TRIM44高表达组患者3年生存率低于TRIM44低表达组(P<0.05);生存患者与死亡患者在肿瘤分化程度(低分化)、淋巴结转移及远处转移比例方面存在显著差异。(P<0.05)。淋巴结转移(HR=3.332)、TRIM44 m RNA高表达(HR=5.365)是影响LSCC患者预后不良的危险因素(P<0.05)。结论 TRIM44 m RNA在LSCC组织中呈高表达,与LSCC患者临床特征及生存情况有关,有望成为潜在的LSCC预后生物标志物。
